Spg002049.1 (mRNA) Sponge gourd (cylindrica) v1

Overview
NameSpg002049.1
TypemRNA
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionGpi-anchor transamidase, putative
Locationscaffold2: 25347020 .. 25351955 (-)
Sequence length636
RNA-Seq ExpressionSpg002049.1
SyntenySpg002049.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAATTTGAGAAAATAAAAAATGAAAAATGAAAAAAAAAAAAAAGAACCCGATGTTGTCGCGTCTCGATTCATTTCTCTGATATTTTTCAACTTCACCCCCCTTTTTTTTTTTTGTAATTTTTTGTAATTTTCTTTTTACTTCTCCCTAAAAAACCCCTATCCAGCCATCTTCTCCTCCTTTTTGTTTCAGCCAGCAGCACGTCTCCCTCTCTCCGACGTCGTCTTCCTCCCCTCCGGCGAGCGGCTCCACGGCGGTCACAGCGGCGGCGGTTCAGCGTTGGAGCAGTCCTTCACGCGCAGCAGTGGGTTTTTTTTTTCTTTTCCTCGCGATTTTCGTCTCGGTTTTTCTCTCTCAATCTTCCCCTCTCAATTTTCGGCTAGGTTTCAAGTTCGATTTGGTGGTTTTCCGGCGAGTTTTCAGGCGGGCAGTGGCGGTTTCGTGTTGTTCCGGCAGGTGAACAGTATAGGCGCGTGAGCAGCAGCGTTTTCGTTTCCAGCAGTGGGTAAGAGTTATTAGGTTGATTTTCGGTTGATTATGATGTGTAATCAAATACCCATTGATTGATTCTTGTTATTGGATTCAAGGTACCCCTAGCATTTTGGAGTTTAATTGTCGAATTAAGGTTAGATTGGAAGCTTGGACAACAAGCTTTGTCTCTGTTTTGGGTAAGATTTGGGGTTAGTTCTTGTCCCCAAATTTATTGGCTCGTGGTTGAATGGTTGGATTAAACCCCCATTGATTTGAGATGATCTTTCTCCTTTTTGTGTAGTTGACTAAGGGCTTTGGAGCTAAGGAGTAAATATAGTTTTTATCAAGCAATTATTGGCTTAGGTAAGTAGCCATGTGGATACAATTTCCATGCTTTAGACCCATTGCATGATTGTTGGAATTGATAAGATTAAGTTCTATGCTTTAGACTAATTTTCTTTGAGAATTTAGTATGCTAGCATGAATTAGATTTAAGCCATGTGTTTTGCTTTTGGATTCAAATGAGCATGACGAGTTTCATTGTGAAGCATGATGACAGTGTGAGATATTTATAGTTAATTGTAGCATGTTGAGACTGCCTTCAGTATGCTTTTAGTTCCTTTTAATCGGGTCGTTACAGTTGAGTTTTTTACAAGGGCAATAAAATCTTGACCCAACTGAAGTTTTCTGGTGCAGCTTTAGAAGATGTTCAAGTTTTGCAGTTCTACAATGGTTTTACCTTTGATTCTGATTCTTGGTCTTGGTTATTTGGGCAACTGTATGGCTTATGGTTCTCCTTCCGAGACAACTATGCATACCAATAACTGGGCAGTTCTAGTCTGTACCTCTCGCTACTGGTTAGTCCAAATCGATTCCAACCTTTTGGTATTTGCGTTTCTTGTGAATTGATAATGGTTCGACTTGTTGGTAGTCGAGATTGTCTAATTTTTATTGTCAAAGAATGTAATGTTCGATTACTTGGATGGATGCTTGATGATGGGTGTTCACTTGAGTGAGTTAGCTTCATCAGTTCTATCTTTCCTGCAATAGATTTTAGGATGAGATTAATCCAACTCGGTGGATCAAATCAGTAAATTAGCGGTCTTAATTACATTTTAGATTTGTTTCAGATCCAAAGTTATATTACTGTTTGGAGAATAGATTATCATGTGTGATAAGTTGCGCCAAGTTCTTGTCTATGGTGTATATTTTAATGGATTATTTCAGTAAGTTGTTGACCTTGAAAGGTGATTGTAATCTATTACAAATTTTCAACAGCTTCAAGATATAATAGTTCATTCCAGTTACCATCTTAAATAAGTTGCGGTTGTGGTCTTTCAGGTTCAATTATCGGCACATGGCTAATACACTATCCTTGTACAGGTTAGACAATAGCTTTGGCGATCTATTTTCCTTGTAAGACCTAGCTGGGTGATTGTCAAATTGGTATCTTCTTTGCATTTGTTTTAGGACTGTGAAACGGCTAGGAATACCTGATGAGAGGATTATACTTATGTTGGCGGATGACATAGCCTGCAATCCTCGAAACAAATACCCTGCAAAAGTGTTCAACAATGAAAATCACAAAATCAATTTGTATGGAGATAATGTTGAGGTTGGTTTCTCTGCTCAATGTTTTTCTATCTAAAAGCAAACAATAGCTAGCTGTGTTACATTTTGGTAATGGATCTTATTTGCACCTGACCAACAACTTGATGATGTTGGTTTGCTAATTGTTCTAATTTTTAGCCACTTTCTTGAATATCATTATCAAATATTAGCCGATGCCATCACCATGGGATTCTGGGGGATTGTTAGTTAAAAATAATACCATGACCTTCTGGATACCTGATAAAAGATCAATGGAACTATGCTGTTTTCTGCCACCAGTTGTATTTTCTATGGCCTTCCTCCATTTGGGTTCTAGGCTTTTATTGCTCTGATACTGATTATTATTACATTGATTTTGATTGTGGTTATTTATTTATTTATTTATTTTTTAATTTTTTAATTTGAAATATAAAATCAATCTTCTATTTTTTTTTATAGGAAACATAAACCATTTCATTGATGTATGAAATAGATACAAAAGGAAGACCTATCAATGATAGTTACAAAAGACATCTCCAATGAGATAATAGGTAGATAAACTGTAATTACAAAAGGGTGGAGAGTGACTACTCCAATAAAGAGCTAAGTAGGATGTGGATTCAATGAAAGCATAAAGATTTTGAGACTTTTCTGTGAAAATCCTAGCATTTCGTTCCAGCCACAAATTCCAAAAAAAAGCATAAATAAAGTTGCGCCATAGGAAATTATTCTCTTTTTTAAAAGGCTGTCCAATGAGAGAAAGCTTGAGAAGCGAGAGGATATGTCCCGGTCTAACACATTGCCATCCAAATGCTTGTTGCAAGAAGTCCCATACGACTAAAGCAAAAGGGCAGTTGCTGAAAAGGTGGATTTGAGTTTCTTGATCCCTATAACAGGAGGAGCAGCAGCTAGGGGAAAGAGCAATCCCAAGGGATTTTCGTTGAAGCACATCCGCCGTGTTGATGCATGCGTGGCTAAGTTCCCACATAAAAAATTTGACCTTTTTTTGGCATAGGTCCCATCCAAAGCTGACTGTAGAGATCAAAGTTAATGCGATTGCCAGTTGAAGCCAGCTTTTTAGTAAGTGAGCCCATAGAGAAAATACTAGCTTTGTCAAGATTCCAAATCCATTCCTCCTCTTTTAGGGAGAAACTAGATAGCTGGTGGGAAAGTATGACCCATTCTAGGGTCTCCTCTTCATTTAGGATTCTTCTCAAATGTAGGTTCCATGCTTCTATGGAAGGATTCCAGAATTCAGCTATGTTGGCCTCTTTTTCTTGTGATAGGTGGAATAAAGAGGGGAATTTCGATTGCAGACTTGTGGAACTGAGCCATACATCTCCCCAAAATTTTGTCTTGTCCCCCTTTCCAACTTTAACATCAATATTATTATAAATCAACTGTTGCATCTTTTGAATAGATTTCCAGGGACCCTTTGATGTGCCAAGCGTTTTCCTGCCCGGTTTGCAGCCTTGTTTGTTTCTTATCAAAAAAAAAAAAAAAAGATATTCTTGCTGTGTATATCATAGAGACCCAAACCCCCCACCTCCATAGGGGTCTTTATGTGAGACCATTTTAATAGATGACTACCTCTTTTTCCAAACCCCCTTTCCAAAGAAAGGTTCTATACAACTTTTCTATAGAGAGAGAGTAAAAATCAATCTTCTATGAACAGCAGGGCTGTACTTGTGGTTATAAAAAAATCAATTTGCTATAGAAAAGGTAAAATGAACAAAATGATACGTTACATCACATATCAATCTTCTATAAATGATGTCGTTTGCTTTTGCTTATTAATATTTTAATTCTTTAAGAACTTTCCACGTTTTTTCTACACCATATATCGTAGTGATTTTATTTTATTATTTTTTATATGGTGATTCTCGTTAATCATAGTTCAAGACATTCCGTGAGTCATGATTTCTTGAAACAATTTATTGTCTTGGCTGCACTTAGGTTGATTATCGAGGTTATGAAGTCACAGTTGAAAATTTTTTAAGGGTATTGACTGGACGCCATGAGGCTGCTGTTCCAAGATCAAAGCGGTTGTTAAGTGACGAAGGAAGCCACATTCTTCTATACATGACAGGACATGGAGGAGATGAATTTTTGAAGTTCCAGGACTCTGAAGAGCTCCAAAGCCATGATTTAGCTGATGCCGTGAAACAAATGAAAGAAAAACATAGGTGAGAATGTTTTTTGTATGGGCTTACAGCACCAATGTTAATTGGATTGGTTTATAGTAAAAAAAAACGTTAGCAAGGTTTACAATTAATTGAAGTTTTGCTGTTTTAGTCATCCAGATGGCTTGTCTAATGATGAATAAATAAACTATAAGCTTACATGTCTTTGAAGTTAGATTTTAGTTTTCACTGTGAATTACTTAGGATGTTAAATGTCCTTTAAGTTTCTCAAATATCAAATTTAATGGGTTTAGATGGCCGTGTCTTGTGATTAGTCCAAGTTCATGAAAATTGGATCAAAGCCAACAATTCTGAATAAAATGAATCAGGGTTTGGAACTGTATAACCATCGAAAGCCATTTGTTCTGCAGATTTATTGGAAACTATTTTTCTTTCTTCTTTACTATCAATTTTTATTTTCTTACTTTAAATACACTTTGCTGTCATTAATGTTGGTGGAGATATTATACTGATAGATTTAAGGAGCTGCTAATAATGGTTGATACTTGCCAAGCTGCTACTCTCTTCAATCAGGTTTGCAATAACTCAGTCTTTCAATTCTGTTAGTGGGTTTGGCTGACAAGATAGATGTAGATTTATTGAAACTAAGATTAGAGAGTTGAACCAACATTTGGTTTGTAAGTTTGTTTATCCTGGACTGTGTCTTTCTTCTAATTTTGTAGGCTATATAG

mRNA sequence

ATGTTCAAGTTTTGCAGTTCTACAATGGTTTTACCTTTGATTCTGATTCTTGGTCTTGGTTATTTGGGCAACTGTATGGCTTATGGTTCTCCTTCCGAGACAACTATGCATACCAATAACTGGGCAGTTCTAGTCTGTACCTCTCGCTACTGGTTCAATTATCGGCACATGGCTAATACACTATCCTTGTACAGGACTGTGAAACGGCTAGGAATACCTGATGAGAGGATTATACTTATGTTGGCGGATGACATAGCCTGCAATCCTCGAAACAAATACCCTGCAAAAGTGTTCAACAATGAAAATCACAAAATCAATTTGTATGGAGATAATGTTGAGGTTGATTATCGAGGTTATGAAGTCACAGTTGAAAATTTTTTAAGGGTATTGACTGGACGCCATGAGGCTGCTGTTCCAAGATCAAAGCGGTTGTTAAGTGACGAAGGAAGCCACATTCTTCTATACATGACAGGACATGGAGGAGATGAATTTTTGAAGTTCCAGGACTCTGAAGAGCTCCAAAGCCATGATTTAGCTGATGCCGTGAAACAAATGAAAGAAAAACATAGATTTAAGGAGCTGCTAATAATGGTTGATACTTGCCAAGCTGCTACTCTCTTCAATCAGGCTATATAG

Coding sequence (CDS)

ATGTTCAAGTTTTGCAGTTCTACAATGGTTTTACCTTTGATTCTGATTCTTGGTCTTGGTTATTTGGGCAACTGTATGGCTTATGGTTCTCCTTCCGAGACAACTATGCATACCAATAACTGGGCAGTTCTAGTCTGTACCTCTCGCTACTGGTTCAATTATCGGCACATGGCTAATACACTATCCTTGTACAGGACTGTGAAACGGCTAGGAATACCTGATGAGAGGATTATACTTATGTTGGCGGATGACATAGCCTGCAATCCTCGAAACAAATACCCTGCAAAAGTGTTCAACAATGAAAATCACAAAATCAATTTGTATGGAGATAATGTTGAGGTTGATTATCGAGGTTATGAAGTCACAGTTGAAAATTTTTTAAGGGTATTGACTGGACGCCATGAGGCTGCTGTTCCAAGATCAAAGCGGTTGTTAAGTGACGAAGGAAGCCACATTCTTCTATACATGACAGGACATGGAGGAGATGAATTTTTGAAGTTCCAGGACTCTGAAGAGCTCCAAAGCCATGATTTAGCTGATGCCGTGAAACAAATGAAAGAAAAACATAGATTTAAGGAGCTGCTAATAATGGTTGATACTTGCCAAGCTGCTACTCTCTTCAATCAGGCTATATAG

Protein sequence

MFKFCSSTMVLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANTLSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYEVTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLADAVKQMKEKHRFKELLIMVDTCQAATLFNQAI
Homology
BLAST of Spg002049.1 vs. NCBI nr
Match: XP_022158481.1 (putative GPI-anchor transamidase isoform X1 [Momordica charantia])

HSP 1 Score: 416.4 bits (1069), Expect = 1.5e-112
Identity = 201/209 (96.17%), Postives = 204/209 (97.61%), Query Frame = 0

Query: 1   MFKFCSSTMVLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANT 60
           M+ FCSSTM LPLILILGLGY+GNCMAYGSPS+TTMHTNNWAVLVCTSRYWFNYRHMANT
Sbjct: 23  MYNFCSSTMALPLILILGLGYMGNCMAYGSPSQTTMHTNNWAVLVCTSRYWFNYRHMANT 82

Query: 61  LSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYE 120
           LSLYRTVKRLGIPDERIILMLADDIACN RNKYPAKVFNNEN KINLYGDNVEVDYRGYE
Sbjct: 83  LSLYRTVKRLGIPDERIILMLADDIACNARNKYPAKVFNNENQKINLYGDNVEVDYRGYE 142

Query: 121 VTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 180
           VTVENFLRVLTGRHE AVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD
Sbjct: 143 VTVENFLRVLTGRHEVAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 202

Query: 181 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 210
           AVKQMKEKHRFKELLIMVDTCQAATLFNQ
Sbjct: 203 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 231

BLAST of Spg002049.1 vs. NCBI nr
Match: XP_022158482.1 (putative GPI-anchor transamidase isoform X2 [Momordica charantia])

HSP 1 Score: 416.4 bits (1069), Expect = 1.5e-112
Identity = 201/209 (96.17%), Postives = 204/209 (97.61%), Query Frame = 0

Query: 1   MFKFCSSTMVLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANT 60
           M+ FCSSTM LPLILILGLGY+GNCMAYGSPS+TTMHTNNWAVLVCTSRYWFNYRHMANT
Sbjct: 23  MYNFCSSTMALPLILILGLGYMGNCMAYGSPSQTTMHTNNWAVLVCTSRYWFNYRHMANT 82

Query: 61  LSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYE 120
           LSLYRTVKRLGIPDERIILMLADDIACN RNKYPAKVFNNEN KINLYGDNVEVDYRGYE
Sbjct: 83  LSLYRTVKRLGIPDERIILMLADDIACNARNKYPAKVFNNENQKINLYGDNVEVDYRGYE 142

Query: 121 VTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 180
           VTVENFLRVLTGRHE AVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD
Sbjct: 143 VTVENFLRVLTGRHEVAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 202

Query: 181 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 210
           AVKQMKEKHRFKELLIMVDTCQAATLFNQ
Sbjct: 203 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 231

BLAST of Spg002049.1 vs. NCBI nr
Match: XP_004142526.1 (GPI-anchor transamidase [Cucumis sativus] >KGN66792.1 hypothetical protein Csa_007570 [Cucumis sativus])

HSP 1 Score: 410.6 bits (1054), Expect = 8.1e-111
Identity = 199/209 (95.22%), Postives = 204/209 (97.61%), Query Frame = 0

Query: 1   MFKFCSSTMVLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANT 60
           M++F +S MV PLILILGLG LGNCMAY SPSETT+HTNNWAVLVCTSRYWFNYRHMANT
Sbjct: 1   MYQFYTSKMVFPLILILGLGVLGNCMAYASPSETTLHTNNWAVLVCTSRYWFNYRHMANT 60

Query: 61  LSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYE 120
           LSLYRTVKRLGIPDERIILMLADDIACNPRNKYPA+VFNNENHKINLYGDNVEVDYRGYE
Sbjct: 61  LSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAEVFNNENHKINLYGDNVEVDYRGYE 120

Query: 121 VTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 180
           VTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD
Sbjct: 121 VTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 180

Query: 181 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 210
           AVKQMKEKHRFKELLIMVDTCQAATLFNQ
Sbjct: 181 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 209

BLAST of Spg002049.1 vs. NCBI nr
Match: XP_038878815.1 (putative GPI-anchor transamidase [Benincasa hispida])

HSP 1 Score: 410.2 bits (1053), Expect = 1.1e-110
Identity = 198/209 (94.74%), Postives = 203/209 (97.13%), Query Frame = 0

Query: 1   MFKFCSSTMVLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANT 60
           M++F +S MVLPLILI+GL Y GNCMAY SPSETTMHTNNWAVLVCTSRYWFNYRHMANT
Sbjct: 1   MYQFYTSKMVLPLILIMGLAYFGNCMAYASPSETTMHTNNWAVLVCTSRYWFNYRHMANT 60

Query: 61  LSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYE 120
           LSLYRTVKRLGIPDERIILMLADDIACNPRNKYPA+VFNNENHKINLYGDNVEVDYRGYE
Sbjct: 61  LSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAEVFNNENHKINLYGDNVEVDYRGYE 120

Query: 121 VTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 180
           VTVENFLRVLTGRHE AVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD
Sbjct: 121 VTVENFLRVLTGRHEVAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 180

Query: 181 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 210
           AVKQMKEKHRFKELLIMVDTCQAATLFNQ
Sbjct: 181 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 209

BLAST of Spg002049.1 vs. NCBI nr
Match: KAG7033435.1 (GPI8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 404.8 bits (1039), Expect = 4.5e-109
Identity = 201/211 (95.26%), Postives = 204/211 (96.68%), Query Frame = 0

Query: 1   MFKFCSSTM--VLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMA 60
           M +F SS+M  +L LILILGLGYLGN MAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMA
Sbjct: 1   MSQFYSSSMALLLILILILGLGYLGNSMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMA 60

Query: 61  NTLSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRG 120
           NTLSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRG
Sbjct: 61  NTLSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRG 120

Query: 121 YEVTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDL 180
           YEVTVENFLRVLTGRHE AVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDL
Sbjct: 121 YEVTVENFLRVLTGRHEVAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDL 180

Query: 181 ADAVKQMKEKHRFKELLIMVDTCQAATLFNQ 210
           ADAVKQMKEKHRFKELLIMVDTCQAATLFNQ
Sbjct: 181 ADAVKQMKEKHRFKELLIMVDTCQAATLFNQ 211

BLAST of Spg002049.1 vs. ExPASy Swiss-Prot
Match: Q5R6L8 (GPI-anchor transamidase OS=Pongo abelii OX=9601 GN=PIGK PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 2.8e-69
Identity = 126/204 (61.76%), Postives = 161/204 (78.92%), Query Frame = 0

Query: 6   SSTMVLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANTLSLYR 65
           +ST+   L+L  G     +          + HTNNWAVLVCTSR+WFNYRH+ANTLS+YR
Sbjct: 11  ASTLAAVLLLSFGSVAASHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYR 70

Query: 66  TVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYEVTVEN 125
           +VKRLGIPD  I+LMLADD+ACNPRN  PA VF+++N ++N+YGD+VEVDYR YEVTVEN
Sbjct: 71  SVKRLGIPDSHIVLMLADDMACNPRNPKPATVFSHKNMELNVYGDDVEVDYRSYEVTVEN 130

Query: 126 FLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLADAVKQM 185
           FLRVLTGR   + PRSKRLLSD+ S+IL+YMTGHGG+ FLKFQDSEE+ + +LADA +QM
Sbjct: 131 FLRVLTGRIPPSTPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQM 190

Query: 186 KEKHRFKELLIMVDTCQAATLFNQ 210
            +K R+ ELL ++DTCQ A+++ +
Sbjct: 191 WQKRRYNELLFIIDTCQGASMYER 214

BLAST of Spg002049.1 vs. ExPASy Swiss-Prot
Match: Q92643 (GPI-anchor transamidase OS=Homo sapiens OX=9606 GN=PIGK PE=1 SV=2)

HSP 1 Score: 262.7 bits (670), Expect = 3.6e-69
Identity = 121/173 (69.94%), Postives = 151/173 (87.28%), Query Frame = 0

Query: 37  HTNNWAVLVCTSRYWFNYRHMANTLSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAK 96
           HTNNWAVLVCTSR+WFNYRH+ANTLS+YR+VKRLGIPD  I+LMLADD+ACNPRN  PA 
Sbjct: 42  HTNNWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNPRNPKPAT 101

Query: 97  VFNNENHKINLYGDNVEVDYRGYEVTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYM 156
           VF+++N ++N+YGD+VEVDYR YEVTVENFLRVLTGR   + PRSKRLLSD+ S+IL+YM
Sbjct: 102 VFSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPPSTPRSKRLLSDDRSNILIYM 161

Query: 157 TGHGGDEFLKFQDSEELQSHDLADAVKQMKEKHRFKELLIMVDTCQAATLFNQ 210
           TGHGG+ FLKFQDSEE+ + +LADA +QM +K R+ ELL ++DTCQ A+++ +
Sbjct: 162 TGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYER 214

BLAST of Spg002049.1 vs. ExPASy Swiss-Prot
Match: Q3MHZ7 (GPI-anchor transamidase OS=Bos taurus OX=9913 GN=PIGK PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 4.7e-69
Identity = 120/173 (69.36%), Postives = 152/173 (87.86%), Query Frame = 0

Query: 37  HTNNWAVLVCTSRYWFNYRHMANTLSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAK 96
           HTNNWAVLVCTSR+WFNYRH+ANTLS+YR+VKRLGIPD  I+LMLADD+ACNPRN  PA 
Sbjct: 42  HTNNWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNPRNPKPAT 101

Query: 97  VFNNENHKINLYGDNVEVDYRGYEVTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYM 156
           V++++N ++N+YGD+VEVDYR YEVTVENFLRVLTGR  ++ PRSKRLLSD+ S+IL+YM
Sbjct: 102 VYSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPSSTPRSKRLLSDDRSNILIYM 161

Query: 157 TGHGGDEFLKFQDSEELQSHDLADAVKQMKEKHRFKELLIMVDTCQAATLFNQ 210
           TGHGG+ FLKFQDSEE+ + +LADA +QM +K R+ ELL ++DTCQ A+++ +
Sbjct: 162 TGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYER 214

BLAST of Spg002049.1 vs. ExPASy Swiss-Prot
Match: Q9CXY9 (GPI-anchor transamidase OS=Mus musculus OX=10090 GN=Pigk PE=1 SV=2)

HSP 1 Score: 260.8 bits (665), Expect = 1.4e-68
Identity = 125/203 (61.58%), Postives = 160/203 (78.82%), Query Frame = 0

Query: 7   STMVLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANTLSLYRT 66
           +T+    +L LG    G+          + HTNNWAVLVCTSR+WFNYRH+ANTLS+YR+
Sbjct: 12  ATLAALALLSLGSSAAGHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYRS 71

Query: 67  VKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYEVTVENF 126
           VKRLGIPD  I+LMLADD+ACN RN  PA VF+++N ++N+YGD+VEVDYR YEVTVENF
Sbjct: 72  VKRLGIPDSHIVLMLADDMACNARNPKPATVFSHKNMELNVYGDDVEVDYRSYEVTVENF 131

Query: 127 LRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLADAVKQMK 186
           LRVLTGR   + PRSKRLLSD+ S+IL+YMTGHGG+ FLKFQDSEE+ + +LADA +QM 
Sbjct: 132 LRVLTGRVPPSTPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMW 191

Query: 187 EKHRFKELLIMVDTCQAATLFNQ 210
           +K R+ ELL ++DTCQ A+++ +
Sbjct: 192 QKRRYNELLFIIDTCQGASMYER 214

BLAST of Spg002049.1 vs. ExPASy Swiss-Prot
Match: P49018 (GPI-anchor transamidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GPI8 PE=1 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 1.4e-68
Identity = 129/205 (62.93%), Postives = 160/205 (78.05%), Query Frame = 0

Query: 9   MVLPLIL----ILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANTLSLY 68
           M LPL+L    +L L    N  A      T  +TNNWAVLV TSR+WFNYRHMAN LS+Y
Sbjct: 5   MHLPLLLLYIFLLPLSGANNTDAAHEVIAT--NTNNWAVLVSTSRFWFNYRHMANVLSMY 64

Query: 69  RTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYEVTVE 128
           RTVKRLGIPD +IILML+DD+ACN RN +P  VFNN++H I+LYGD+VEVDYRGYEVTVE
Sbjct: 65  RTVKRLGIPDSQIILMLSDDVACNSRNLFPGSVFNNKDHAIDLYGDSVEVDYRGYEVTVE 124

Query: 129 NFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLADAVKQ 188
           NF+R+LT R     P+SKRLL+DE S+I +YMTGHGGD+FLKFQD+EE+ S D+ADA +Q
Sbjct: 125 NFIRLLTDRWTEDHPKSKRLLTDENSNIFIYMTGHGGDDFLKFQDAEEIASEDIADAFQQ 184

Query: 189 MKEKHRFKELLIMVDTCQAATLFNQ 210
           M EK R+ E+  M+DTCQA T++++
Sbjct: 185 MYEKKRYNEIFFMIDTCQANTMYSK 207

BLAST of Spg002049.1 vs. ExPASy TrEMBL
Match: A0A6J1E111 (putative GPI-anchor transamidase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111024963 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 7.2e-113
Identity = 201/209 (96.17%), Postives = 204/209 (97.61%), Query Frame = 0

Query: 1   MFKFCSSTMVLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANT 60
           M+ FCSSTM LPLILILGLGY+GNCMAYGSPS+TTMHTNNWAVLVCTSRYWFNYRHMANT
Sbjct: 23  MYNFCSSTMALPLILILGLGYMGNCMAYGSPSQTTMHTNNWAVLVCTSRYWFNYRHMANT 82

Query: 61  LSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYE 120
           LSLYRTVKRLGIPDERIILMLADDIACN RNKYPAKVFNNEN KINLYGDNVEVDYRGYE
Sbjct: 83  LSLYRTVKRLGIPDERIILMLADDIACNARNKYPAKVFNNENQKINLYGDNVEVDYRGYE 142

Query: 121 VTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 180
           VTVENFLRVLTGRHE AVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD
Sbjct: 143 VTVENFLRVLTGRHEVAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 202

Query: 181 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 210
           AVKQMKEKHRFKELLIMVDTCQAATLFNQ
Sbjct: 203 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 231

BLAST of Spg002049.1 vs. ExPASy TrEMBL
Match: A0A6J1DXC4 (putative GPI-anchor transamidase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111024963 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 7.2e-113
Identity = 201/209 (96.17%), Postives = 204/209 (97.61%), Query Frame = 0

Query: 1   MFKFCSSTMVLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANT 60
           M+ FCSSTM LPLILILGLGY+GNCMAYGSPS+TTMHTNNWAVLVCTSRYWFNYRHMANT
Sbjct: 23  MYNFCSSTMALPLILILGLGYMGNCMAYGSPSQTTMHTNNWAVLVCTSRYWFNYRHMANT 82

Query: 61  LSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYE 120
           LSLYRTVKRLGIPDERIILMLADDIACN RNKYPAKVFNNEN KINLYGDNVEVDYRGYE
Sbjct: 83  LSLYRTVKRLGIPDERIILMLADDIACNARNKYPAKVFNNENQKINLYGDNVEVDYRGYE 142

Query: 121 VTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 180
           VTVENFLRVLTGRHE AVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD
Sbjct: 143 VTVENFLRVLTGRHEVAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 202

Query: 181 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 210
           AVKQMKEKHRFKELLIMVDTCQAATLFNQ
Sbjct: 203 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 231

BLAST of Spg002049.1 vs. ExPASy TrEMBL
Match: A0A0A0M0Z0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690290 PE=3 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 3.9e-111
Identity = 199/209 (95.22%), Postives = 204/209 (97.61%), Query Frame = 0

Query: 1   MFKFCSSTMVLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANT 60
           M++F +S MV PLILILGLG LGNCMAY SPSETT+HTNNWAVLVCTSRYWFNYRHMANT
Sbjct: 1   MYQFYTSKMVFPLILILGLGVLGNCMAYASPSETTLHTNNWAVLVCTSRYWFNYRHMANT 60

Query: 61  LSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYE 120
           LSLYRTVKRLGIPDERIILMLADDIACNPRNKYPA+VFNNENHKINLYGDNVEVDYRGYE
Sbjct: 61  LSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAEVFNNENHKINLYGDNVEVDYRGYE 120

Query: 121 VTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 180
           VTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD
Sbjct: 121 VTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLAD 180

Query: 181 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 210
           AVKQMKEKHRFKELLIMVDTCQAATLFNQ
Sbjct: 181 AVKQMKEKHRFKELLIMVDTCQAATLFNQ 209

BLAST of Spg002049.1 vs. ExPASy TrEMBL
Match: A0A6J1H9N5 (putative GPI-anchor transamidase OS=Cucurbita moschata OX=3662 GN=LOC111461778 PE=3 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.2e-109
Identity = 201/211 (95.26%), Postives = 204/211 (96.68%), Query Frame = 0

Query: 1   MFKFCSSTM--VLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMA 60
           M +F SS+M  +L LILILGLGYLGN MAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMA
Sbjct: 1   MSQFYSSSMALLLILILILGLGYLGNSMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMA 60

Query: 61  NTLSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRG 120
           NTLSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRG
Sbjct: 61  NTLSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRG 120

Query: 121 YEVTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDL 180
           YEVTVENFLRVLTGRHE AVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDL
Sbjct: 121 YEVTVENFLRVLTGRHEVAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDL 180

Query: 181 ADAVKQMKEKHRFKELLIMVDTCQAATLFNQ 210
           ADAVKQMKEKHRFKELLIMVDTCQAATLFNQ
Sbjct: 181 ADAVKQMKEKHRFKELLIMVDTCQAATLFNQ 211

BLAST of Spg002049.1 vs. ExPASy TrEMBL
Match: A0A6J1JC91 (putative GPI-anchor transamidase OS=Cucurbita maxima OX=3661 GN=LOC111485473 PE=3 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 9.1e-108
Identity = 199/211 (94.31%), Postives = 202/211 (95.73%), Query Frame = 0

Query: 1   MFKFCSSTM--VLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMA 60
           M +F SS+M  +L LILIL LGYLGN MAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMA
Sbjct: 1   MSQFYSSSMALLLILILILCLGYLGNSMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMA 60

Query: 61  NTLSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRG 120
           NTLSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRG
Sbjct: 61  NTLSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRG 120

Query: 121 YEVTVENFLRVLTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDL 180
           YEVTVENFLRVL GRHE AVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDL
Sbjct: 121 YEVTVENFLRVLAGRHEVAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDL 180

Query: 181 ADAVKQMKEKHRFKELLIMVDTCQAATLFNQ 210
           ADAVKQMKEKHRFKELLIMVDTCQAATLFNQ
Sbjct: 181 ADAVKQMKEKHRFKELLIMVDTCQAATLFNQ 211

BLAST of Spg002049.1 vs. TAIR 10
Match: AT1G08750.1 (Peptidase C13 family )

HSP 1 Score: 349.0 bits (894), Expect = 2.7e-96
Identity = 169/200 (84.50%), Postives = 184/200 (92.00%), Query Frame = 0

Query: 10  VLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANTLSLYRTVKR 69
           +L L+++L   ++       S  +TT+HTNNWAVLVCTSR+WFNYRHMANTLSLYRTVKR
Sbjct: 3   ILTLVMLLCYSFV------SSTGDTTIHTNNWAVLVCTSRFWFNYRHMANTLSLYRTVKR 62

Query: 70  LGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYEVTVENFLRV 129
           LGIPDERIILMLADD+ACN RN+YPA+VFNNENHK+NLYGDNVEVDYRGYEVTVENFLRV
Sbjct: 63  LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV 122

Query: 130 LTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLADAVKQMKEKH 189
           LTGRHE AVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQD+EELQSHDLADAVKQMKEK 
Sbjct: 123 LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 182

Query: 190 RFKELLIMVDTCQAATLFNQ 210
           RFKEL+IMVDTCQAATLFNQ
Sbjct: 183 RFKELMIMVDTCQAATLFNQ 196

BLAST of Spg002049.1 vs. TAIR 10
Match: AT1G08750.2 (Peptidase C13 family )

HSP 1 Score: 349.0 bits (894), Expect = 2.7e-96
Identity = 169/200 (84.50%), Postives = 184/200 (92.00%), Query Frame = 0

Query: 10  VLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANTLSLYRTVKR 69
           +L L+++L   ++       S  +TT+HTNNWAVLVCTSR+WFNYRHMANTLSLYRTVKR
Sbjct: 3   ILTLVMLLCYSFV------SSTGDTTIHTNNWAVLVCTSRFWFNYRHMANTLSLYRTVKR 62

Query: 70  LGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYEVTVENFLRV 129
           LGIPDERIILMLADD+ACN RN+YPA+VFNNENHK+NLYGDNVEVDYRGYEVTVENFLRV
Sbjct: 63  LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV 122

Query: 130 LTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLADAVKQMKEKH 189
           LTGRHE AVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQD+EELQSHDLADAVKQMKEK 
Sbjct: 123 LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 182

Query: 190 RFKELLIMVDTCQAATLFNQ 210
           RFKEL+IMVDTCQAATLFNQ
Sbjct: 183 RFKELMIMVDTCQAATLFNQ 196

BLAST of Spg002049.1 vs. TAIR 10
Match: AT1G08750.3 (Peptidase C13 family )

HSP 1 Score: 349.0 bits (894), Expect = 2.7e-96
Identity = 169/200 (84.50%), Postives = 184/200 (92.00%), Query Frame = 0

Query: 10  VLPLILILGLGYLGNCMAYGSPSETTMHTNNWAVLVCTSRYWFNYRHMANTLSLYRTVKR 69
           +L L+++L   ++       S  +TT+HTNNWAVLVCTSR+WFNYRHMANTLSLYRTVKR
Sbjct: 3   ILTLVMLLCYSFV------SSTGDTTIHTNNWAVLVCTSRFWFNYRHMANTLSLYRTVKR 62

Query: 70  LGIPDERIILMLADDIACNPRNKYPAKVFNNENHKINLYGDNVEVDYRGYEVTVENFLRV 129
           LGIPDERIILMLADD+ACN RN+YPA+VFNNENHK+NLYGDNVEVDYRGYEVTVENFLRV
Sbjct: 63  LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV 122

Query: 130 LTGRHEAAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDSEELQSHDLADAVKQMKEKH 189
           LTGRHE AVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQD+EELQSHDLADAVKQMKEK 
Sbjct: 123 LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 182

Query: 190 RFKELLIMVDTCQAATLFNQ 210
           RFKEL+IMVDTCQAATLFNQ
Sbjct: 183 RFKELMIMVDTCQAATLFNQ 196

BLAST of Spg002049.1 vs. TAIR 10
Match: AT2G25940.1 (alpha-vacuolar processing enzyme )

HSP 1 Score: 106.7 bits (265), Expect = 2.3e-23
Identity = 59/178 (33.15%), Postives = 97/178 (54.49%), Query Frame = 0

Query: 31  PSETTMHTNNWAVLVCTSRYWFNYRHMANTLSLYRTVKRLGIPDERIILMLADDIACNPR 90
           P+E    +  WAVLV  S  ++NYRH A+    Y+ +K+ G+ +E I++ + DDIA N  
Sbjct: 35  PTENDDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEE 94

Query: 91  NKYPAKVFNNENHKINLYGDNVEVDYRGYEVTVENFLRVLTGRHEAAVPRSKRLL-SDEG 150
           N  P  + N+ N + ++Y + V  DY G EV V+N L V+ G   A    S +++ S   
Sbjct: 95  NPRPGVIINSPNGE-DVY-NGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPN 154

Query: 151 SHILLYMTGHGGDEFLKFQDSEELQSHDLADAVKQMKEKHRFKELLIMVDTCQAATLF 208
            HI +Y + HGG   L    S  L ++DL D +K+      +K L+  ++ C++ ++F
Sbjct: 155 DHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIF 210

BLAST of Spg002049.1 vs. TAIR 10
Match: AT1G62710.1 (beta vacuolar processing enzyme )

HSP 1 Score: 95.9 bits (237), Expect = 4.1e-20
Identity = 54/168 (32.14%), Postives = 90/168 (53.57%), Query Frame = 0

Query: 41  WAVLVCTSRYWFNYRHMANTLSLYRTVKRLGIPDERIILMLADDIACNPRNKYPAKVFNN 100
           WAVLV  S  + NYRH A+    Y+ +++ G+ +E I++++ DDIA +P N  P  + N+
Sbjct: 52  WAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNPRPGTLINH 111

Query: 101 ENHKINLYGDNVEVDYRGYEVTVENFLRVLTGRHEAAVPRS-KRLLSDEGSHILLYMTGH 160
            +   ++Y   V  DY G  VT  NF  VL G  +A    S K + S    HI +Y   H
Sbjct: 112 PDGD-DVYA-GVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPNDHIFVYYADH 171

Query: 161 GGDEFLKFQDSEELQSHDLADAVKQMKEKHRFKELLIMVDTCQAATLF 208
           GG   L   ++  + + D  + +K+      +KE++I V+ C++ ++F
Sbjct: 172 GGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGSIF 217

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158481.11.5e-11296.17putative GPI-anchor transamidase isoform X1 [Momordica charantia][more]
XP_022158482.11.5e-11296.17putative GPI-anchor transamidase isoform X2 [Momordica charantia][more]
XP_004142526.18.1e-11195.22GPI-anchor transamidase [Cucumis sativus] >KGN66792.1 hypothetical protein Csa_0... [more]
XP_038878815.11.1e-11094.74putative GPI-anchor transamidase [Benincasa hispida][more]
KAG7033435.14.5e-10995.26GPI8 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q5R6L82.8e-6961.76GPI-anchor transamidase OS=Pongo abelii OX=9601 GN=PIGK PE=2 SV=1[more]
Q926433.6e-6969.94GPI-anchor transamidase OS=Homo sapiens OX=9606 GN=PIGK PE=1 SV=2[more]
Q3MHZ74.7e-6969.36GPI-anchor transamidase OS=Bos taurus OX=9913 GN=PIGK PE=2 SV=1[more]
Q9CXY91.4e-6861.58GPI-anchor transamidase OS=Mus musculus OX=10090 GN=Pigk PE=1 SV=2[more]
P490181.4e-6862.93GPI-anchor transamidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c)... [more]
Match NameE-valueIdentityDescription
A0A6J1E1117.2e-11396.17putative GPI-anchor transamidase isoform X1 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1DXC47.2e-11396.17putative GPI-anchor transamidase isoform X2 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A0A0M0Z03.9e-11195.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690290 PE=3 SV=1[more]
A0A6J1H9N52.2e-10995.26putative GPI-anchor transamidase OS=Cucurbita moschata OX=3662 GN=LOC111461778 P... [more]
A0A6J1JC919.1e-10894.31putative GPI-anchor transamidase OS=Cucurbita maxima OX=3661 GN=LOC111485473 PE=... [more]
Match NameE-valueIdentityDescription
AT1G08750.12.7e-9684.50Peptidase C13 family [more]
AT1G08750.22.7e-9684.50Peptidase C13 family [more]
AT1G08750.32.7e-9684.50Peptidase C13 family [more]
AT2G25940.12.3e-2333.15alpha-vacuolar processing enzyme [more]
AT1G62710.14.1e-2032.14beta vacuolar processing enzyme [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001096Peptidase C13, legumainPRINTSPR00776HEMOGLOBNASEcoord: 142..161
score: 45.43
coord: 191..207
score: 31.2
coord: 115..130
score: 62.23
coord: 41..65
score: 51.48
coord: 66..95
score: 45.36
IPR001096Peptidase C13, legumainPFAMPF01650Peptidase_C13coord: 40..209
e-value: 1.3E-41
score: 142.9
NoneNo IPR availableGENE3D3.40.50.1460coord: 38..211
e-value: 2.9E-82
score: 278.3
NoneNo IPR availablePIRSRPIRSR019663-1PIRSR019663-1coord: 39..209
e-value: 1.7E-38
score: 131.0
IPR028361GPI-anchor transamidasePANTHERPTHR48067GPI-ANCHOR TRANSAMIDASEcoord: 8..209

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spg002049Spg002049gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg002049.1-cdsSpg002049.1-cds-scaffold2:25347020..25347028CDS
Spg002049.1-cdsSpg002049.1-cds-scaffold2:25347178..25347235CDS
Spg002049.1-cdsSpg002049.1-cds-scaffold2:25347709..25347938CDS
Spg002049.1-cdsSpg002049.1-cds-scaffold2:25349870..25350014CDS
Spg002049.1-cdsSpg002049.1-cds-scaffold2:25350102..25350143CDS
Spg002049.1-cdsSpg002049.1-cds-scaffold2:25350628..25350779CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg002049.1-five_prime_utrSpg002049.1-five_prime_utr-scaffold2:25350780..25350782five_prime_UTR
Spg002049.1-five_prime_utrSpg002049.1-five_prime_utr-scaffold2:25351288..25351330five_prime_UTR
Spg002049.1-five_prime_utrSpg002049.1-five_prime_utr-scaffold2:25351451..25351532five_prime_UTR
Spg002049.1-five_prime_utrSpg002049.1-five_prime_utr-scaffold2:25351652..25351881five_prime_UTR
Spg002049.1-five_prime_utrSpg002049.1-five_prime_utr-scaffold2:25351902..25351955five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spg002049.1Spg002049.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016255 attachment of GPI anchor to protein
biological_process GO:0006508 proteolysis
cellular_component GO:0042765 GPI-anchor transamidase complex
molecular_function GO:0003923 GPI-anchor transamidase activity
molecular_function GO:0008233 peptidase activity