Sgr028431.1 (mRNA) Monk fruit (Qingpiguo) v1

Overview
NameSgr028431.1
TypemRNA
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionFSH1 domain-containing protein
Locationtig00153145: 2032239 .. 2037158 (-)
Sequence length708
RNA-Seq ExpressionSgr028431.1
SyntenySgr028431.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAGTGGCAAAAGAGAAAATAAAATGAAGATATTATGCCTTCATGGATTCAGAACCAGTGGGAGTTTCCTGAGAAAGCAACTCGGCAAATGGGATCCTTCCATCTTCGCTCATTTTGATATGGTACATACATTTTTGTTTTTAATTTTGTTAAATTACTAAATTAGTTTCGATATTTAAATGTGTTGTGTATCAGATTTGACGGTTTAGATCTGTTTTAAGAAAATGCTTCATTTTTCTCTGTCTTGTGACCCACTTTTCCGTTGGATCCATAGCAATTGATTGGGAAGGGATCATTGCTTGTTCGGGTCAAAGTTTTAAAAGCGTACCATTTTGATTGGAGAAAATTCATTTTTTAAATATGTGCTTACTTGAGTATGTCAACTTCAAACTTAAAATTCCAAATTCATAAAATATAAATTCAACTCCAAACCTAAATAAGTGGTGGAAAGTAATACCCTCAATTAGTTGATTTTCGTTTTTTATTAGATTTGTGATTCTTTTAGTGTGGCAGAGATTATTTTTTTGAATACTAGTTTTTCTATTTTTTTAGCTTGGTTTTAGCATATTGTCATTGAGTTTGAAAGTTTTTTTCTTACCTACTTCATCTAGTTGCAAGGTTTAAATATTTTAAGCTAAATATATCAAATGATCTCTAATATTTTGAGTAATTTTCAATTTGGTCCTTAATTTTTTAAAATTTTCAATTTTACTCCTAATGTATAAAAAGTTCTCAATTATGTCATTCCATAAAAAAATTATTAAAATTTGTTAATAAAAATTTGATATATCATTAAATTATTGTTTTATTAACATTCTTGGACATGTGAATATGCTACTTGAATGCCATTTAACACACAAAACCCTATATGCTCATCAACAAAATCCATTTTTTCTGAGTGGCATACTCTTGTATCTAAATATGTTAACAAAACAATAATTTAATGACACACAACTTTTAATTAACAAATTTTAACATATTTTTTATGGAAGTTGAGAATTTATAATATATTAAGGGTAAAATGGAAAATTTTAAAAGATTAAGGACCAAATTGAGAAAAATTTAAAATATTAGAAACTATATGATATATTTAGCCAATATTTTACATATTTTTAGGTTTGGGCATTTATGTGATATTCGTTAGTTAGTTCTTTAGCAATTTTTAAATTTAATTTTGAGAAATTTTTAATTTTACCACACATTCAAATTTTGAATTGAATTTGATAAAAATGAAAATTTGAATTAAAATTGACTCATCATCTAAGTGGGAAAAACTTAATTTTTACTTGCACTCTTTTAAACAAAGGAGTAAAAAGGTGTAAATCTTCTCACCTCTTTTTTTTTTAAAAAAAAACCTGTAATTCTTCACGTAAATATAATTTTTTTGGAGATTAAACTAAAGATAAAATAATAATCATTGAATTATCGATAATAAAAATCAACTAATATAATTTAAACTTTTAGGATTTTGTAATTTCTAATAGGTCTTCGAATTTTCTAAGCTGTATCTATCTAGTTCTTGTATCTTTTAATTTTGTGTCTTGTAAGTTCCTACTATTAATTCTGTCAGTTTACAATCACGTGACATGTCGAATATATGATTTAAGAATTATGTGACATGTTGATTTCGAAGAGATTAAGACTAGGGATGTCACTTTTTCTGATTTTAATTTCAGGGATGGGGATTGGGGTCTCTGTCCCCATCCTTGACCCCTATGTCGCCCTGTTCCCCGTTCTCCTCTTAAATATACACATTTTTTAAATTTTTTTATAATATATATATATAGACACACACACGTGTTATAAAACTTAAAACTTTTTTTTCACTTTTTAATGAGACATTGTTGTGGTGTTTAAGTTTTAAAATTTATTTGTATTGATAATTTGATATTTAAAACTTGTGAATTTTAGTTCATTTAGACAAGTTTTTTTTATAAAATATTTTGACTAAAATGTAATATTTATTAGATTATTAAAATTAAAATGACATTTATTTTTATTAAAAATTTTAAAACTTTAGCCATTTAAATGAGGATTTCCACAGAGAATTTAACTCAATGAAATGATTGTTCATTTTCCTCAAAAACGAAAAAAAAGTAAATGGAAAAAAAAATTTCGTGGGGAACCCGACCTTAGTGATTTTCCCGCCAAGAATCCCCTCCCTGGTCCCCACGAAATTTAACGAAAAATTTCGCAGGGACAGGGAATGCTATGCGAGGCGGGGATGAGGATGGAGAAGCATCCCCATCCCCACCGCGCTCCCTTGACACCCCTAGTTAAGACATTTAGTGACAGAAACGTAAAAATATAGAGTGAACACTTGAGACCTATTCGATGCAAAATTGAAAGTTCAACGACTAATTAAAATATTTTTTATAGTGTAAGGACTAAATAGATCCAAATCTAAAAATTTAAGGATCTATAAATTTTTTTTTAAAGTATGAAAACTAAATGACTCAATTTTAATAGTTCAGGAACTAAATTTATAATTTAAATTTTTATTTTTTTAAAAGGAAATTTTACAATTTGACTTCCTAAATCAATAATTAACTATTGTCACTAACATGAACTCAAAAAACAAAAAAAAAAAAAAACTATTGTCAATAACAGATTTAGTCGATATATGTATACAATCTACTATTTTTGTCACAATTTCTTTTTACCTATTTTTATAAAATTTACTTCTTGAATTACCAAATTTATTTAGCTGTTTTATGAATTTTCTTTTTTAGCTTCATATCCTTTTGATTTTCCTGGCATAACCAAATTAATTGATCCTTTTATCCATATTAATTTTTTTTTCTCTTTACTATTTTTAACAAAAGTATTTAATTATAGTTATCATTTGTCTATTTAAATAGGAATTTCCAGATGGCATATTTCCCGCAGGAGGAAAATCAGAGATTGAAGGATACTTTCCACCGCCTTATTTTGAATGGTTTCAATTTAACAAGGTACAAATAAATACAATATTTTGTAACTAAAAAAAATTAAATTAAATATTTTAAAGATTTAAAAAAGAATTAAAAAAAAGGGGGTGATATTTTCTTGGCAGGAGTTCACAGAGTACACAAACTTGGAACAATGCATAGATTACTTGTGCAACTATATCACAACCAAGGGCCCATTTGATGGATTGTTGGGCTTTTCTCAGGTTTTTTTCTTTTTTTTTACATTTAAATACTTTTTTGTCCCTAAAATTTATCTTCCTTTCTAATTTAGTTCCTAAAGTTATAAAGACTTAAAATTTTAATAAGTTTCAAACTCAATATTTCGTGTTAAAATCTGAAACAAGAATTAACAAATTTTTTTAATTGTCAATTGACATTCATGTATAATTAATAATTCATGACACGAGAAAAATTGTATAGATATATATTATACTTCACTCAATTAGAAATTGAGATGAGGATGATTGTATATTATACATCATTCAAATAGAAATCGAGAAAAGATAGGGATAAAGCATGCTTGATAGTGAAAAAAGAAGTGGGAAGATTAAAAGAAATCTGACTAGATTATTTTAAGCTTCATGATTGTAAATGTTTTTTTTTAGAATATTACATTACAACTAAGGAGGGTGAGGTTTCAAACAACCTACGACTTCTTAAAGAAAATAGAGATGTTTTAACTATTAAACTATGTTCACGTTGGCAAGATCACAGATGTTAGACAACAAGAAAACGAGTCATGTCACTTTTTTTTTTTTGTTAAAAGATTAATTTTTAACTAATTAAAATTTGAGGAACTAAAATAAAACTTTTTAAGCTTTAGGTATCAAATTAAAAATGAGGGTAAAATATGTTGCAACTTTTTTTTTCTTTTTAAAGAATTCTACATGTATTGTGAGACCTAAATTTTTATTTCAAATTTTGAATCTTTATTCAGGGTGCCACACTGTCAGCACTCTTGTTGGGTTACCAAGCACAGGTACACTTTTATCTTAAAAAGATCGTCCCGAATCTAGCATAATTGAGATAAAATATTACATAAGTAATAATAAAAAACGAAACATACGTCAATTAATGATTTTCATGATTGCAATTAATCAATGATTGCAATGAATAATATAGTTTAATCAATATATAAGTTAATCAATGGTTATGTAAATCAACACACATAAAATAAAAACATATTGATTATTATTTATCAATGATCAATAATTATTTTTTTTCCTAAATACTATATTTACAATTATTTTTAAAGAATATTATATTCAAAATTACTTTTTATCATACTACATATTTAAAAATATGTTAGAAAATGAAGCTATCCAACCGAATTTTTCTAGCTTAAATACCATAAAAAATAGACACAAAAAATATAACATAGTAAAGATTTCATATTATAAAAAAAAAAAAAAAACAAACAACTTAAAATAGTAGTTACAAATACGATATTTTTTTTTTTAAAAAAAAACTATTTTTGTGATAAATATATTACAGAAATTATTGAGAAAGTAGTTTGAAAATGGGAAATTACAGGGAAAGGTGTTGAAGGAGCACCCACCAATCAAAGTGTTTGTGTCAATATCAGGATCAAAATTCAGAGATGCAAGCATATGTGAGGTTGCCTACAAACACAAAATCAAGGCCAAATCTGTTCACTTGATAGGGGAAATGGATTGGTTGAAACTGCCCTCTGAAGAACTTGCTTCTGCCTTCCATGACCCTTTCATCATAAGGCATCCCCAAGGCCATACTGTCCCTAGATTAGGTCATTTTTTACACACATTATCTCTCTCCCTCTCTCTTTCTCTCTCTCTCACTTGATTGGTGGAAAAATGTTGTTGTGTTATAGATGAAGCAGCCACCAGGGAGCTCCGATGCTGGGTCGATGCGGTACTTTGTAGCGATGATCATGCTGGAGACGGAGAAGGAAAGGAAAATTCCATTGATGTAATGATTGAAAATTAA

mRNA sequence

ATGGAGAGTGGCAAAAGAGAAAATAAAATGAAGATATTATGCCTTCATGGATTCAGAACCAGTGGGAGTTTCCTGAGAAAGCAACTCGGCAAATGGGATCCTTCCATCTTCGCTCATTTTGATATGGAATTTCCAGATGGCATATTTCCCGCAGGAGGAAAATCAGAGATTGAAGGATACTTTCCACCGCCTTATTTTGAATGGTTTCAATTTAACAAGGAGTTCACAGAGTACACAAACTTGGAACAATGCATAGATTACTTGTGCAACTATATCACAACCAAGGGCCCATTTGATGGATTGTTGGGCTTTTCTCAGGGTGCCACACTGTCAGCACTCTTGTTGGGTTACCAAGCACAGGGAAAGGTGTTGAAGGAGCACCCACCAATCAAAGTGTTTGTGTCAATATCAGGATCAAAATTCAGAGATGCAAGCATATGTGAGGTTGCCTACAAACACAAAATCAAGGCCAAATCTGTTCACTTGATAGGGGAAATGGATTGGTTGAAACTGCCCTCTGAAGAACTTGCTTCTGCCTTCCATGACCCTTTCATCATAAGGCATCCCCAAGGCCATACTGTCCCTAGATTAGATGAAGCAGCCACCAGGGAGCTCCGATGCTGGGTCGATGCGGTACTTTGTAGCGATGATCATGCTGGAGACGGAGAAGGAAAGGAAAATTCCATTGATGTAATGATTGAAAATTAA

Coding sequence (CDS)

ATGGAGAGTGGCAAAAGAGAAAATAAAATGAAGATATTATGCCTTCATGGATTCAGAACCAGTGGGAGTTTCCTGAGAAAGCAACTCGGCAAATGGGATCCTTCCATCTTCGCTCATTTTGATATGGAATTTCCAGATGGCATATTTCCCGCAGGAGGAAAATCAGAGATTGAAGGATACTTTCCACCGCCTTATTTTGAATGGTTTCAATTTAACAAGGAGTTCACAGAGTACACAAACTTGGAACAATGCATAGATTACTTGTGCAACTATATCACAACCAAGGGCCCATTTGATGGATTGTTGGGCTTTTCTCAGGGTGCCACACTGTCAGCACTCTTGTTGGGTTACCAAGCACAGGGAAAGGTGTTGAAGGAGCACCCACCAATCAAAGTGTTTGTGTCAATATCAGGATCAAAATTCAGAGATGCAAGCATATGTGAGGTTGCCTACAAACACAAAATCAAGGCCAAATCTGTTCACTTGATAGGGGAAATGGATTGGTTGAAACTGCCCTCTGAAGAACTTGCTTCTGCCTTCCATGACCCTTTCATCATAAGGCATCCCCAAGGCCATACTGTCCCTAGATTAGATGAAGCAGCCACCAGGGAGCTCCGATGCTGGGTCGATGCGGTACTTTGTAGCGATGATCATGCTGGAGACGGAGAAGGAAAGGAAAATTCCATTGATGTAATGATTGAAAATTAA

Protein sequence

MESGKRENKMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEGYFPPPYFEWFQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQAQGKVLKEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWLKLPSEELASAFHDPFIIRHPQGHTVPRLDEAATRELRCWVDAVLCSDDHAGDGEGKENSIDVMIEN
Homology
BLAST of Sgr028431.1 vs. NCBI nr
Match: XP_022154224.1 (dihydrofolate reductase-like [Momordica charantia])

HSP 1 Score: 429.5 bits (1103), Expect = 1.9e-116
Identity = 205/240 (85.42%), Postives = 216/240 (90.00%), Query Frame = 0

Query: 1   MESGKRENKMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEGY 60
           MESGKRENKMKILCLHGFRTSGSFLRKQL KWDPSIFAHFD++FPDG+ PAGGKS+IEGY
Sbjct: 1   MESGKRENKMKILCLHGFRTSGSFLRKQLSKWDPSIFAHFDLDFPDGMCPAGGKSDIEGY 60

Query: 61  FPPPYFEWFQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQAQ 120
           FPPPYFEWFQFN+EFTEYTNLEQCI YLCNYITT GPFDGLLGFSQGATLSALLLGYQAQ
Sbjct: 61  FPPPYFEWFQFNQEFTEYTNLEQCIGYLCNYITTNGPFDGLLGFSQGATLSALLLGYQAQ 120

Query: 121 GKVLKEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWLKLPSEELASAF 180
           GKVLKEHPPIK+ VSISGSKFRD  ICEVAYKHKI+AKSVHLIGE DWLKLPS+ELASAF
Sbjct: 121 GKVLKEHPPIKLLVSISGSKFRDEKICEVAYKHKIEAKSVHLIGEKDWLKLPSQELASAF 180

Query: 181 HDPFIIRHPQGHTVPRLDEAATRELRCWVDAVLCSDDHA-GDGEGKEN----SIDVMIEN 236
           HDP IIRHPQGHTVPRLDEAAT+EL+CWVDAVL   DH   D E KEN     +D  IEN
Sbjct: 181 HDPLIIRHPQGHTVPRLDEAATKELQCWVDAVLSGHDHVIEDEEVKENYVIDKMDEKIEN 240

BLAST of Sgr028431.1 vs. NCBI nr
Match: KAG6596208.1 (Esterase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 419.1 bits (1076), Expect = 2.5e-113
Identity = 195/236 (82.63%), Postives = 212/236 (89.83%), Query Frame = 0

Query: 1   MES-GKRENKMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEG 60
           MES GKR  ++KILCLHGFRTSGSFLRKQL KWDPSIFAHFDMEFPD IFP+GGKSEIEG
Sbjct: 1   MESGGKRAERLKILCLHGFRTSGSFLRKQLTKWDPSIFAHFDMEFPDAIFPSGGKSEIEG 60

Query: 61  YFPPPYFEWFQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQA 120
           YFPPPYFEWFQFN++FTEYTNLEQCIDYL NYIT+ GPF GLLGFSQGA LSALLLGYQA
Sbjct: 61  YFPPPYFEWFQFNEDFTEYTNLEQCIDYLNNYITSNGPFHGLLGFSQGAVLSALLLGYQA 120

Query: 121 QGKVLKEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWLKLPSEELASA 180
           QGKVLKEHP +K+FVSISG+KF+D SICEVAYKHKIK KSVHLIGE DWLKLPSEELASA
Sbjct: 121 QGKVLKEHPAMKMFVSISGTKFKDESICEVAYKHKIKVKSVHLIGEKDWLKLPSEELASA 180

Query: 181 FHDPFIIRHPQGHTVPRLDEAATRELRCWVDAVLCSDDHAGDGEGKENSIDVMIEN 236
           FH+P IIRHPQGHTVPRLDE AT++LRCWVDA++ S DH GDGE KEN    + +N
Sbjct: 181 FHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAIVSSHDHVGDGEAKENITQKVADN 236

BLAST of Sgr028431.1 vs. NCBI nr
Match: XP_004136600.1 (esterase OVCA2 [Cucumis sativus] >KGN59361.1 hypothetical protein Csa_001052 [Cucumis sativus])

HSP 1 Score: 418.7 bits (1075), Expect = 3.3e-113
Identity = 192/234 (82.05%), Postives = 213/234 (91.03%), Query Frame = 0

Query: 3   SGKRENK--MKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEGY 62
           S KR++K  +KILCLHGFRT+GSFLRKQ+ KWDPSIFAHFDMEFPDGIFPAGGKSEIEGY
Sbjct: 4   SSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEIEGY 63

Query: 63  FPPPYFEWFQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQAQ 122
           FPPPYFEWFQFN++FTEY NLE+CIDY+CNYITT GPF GLLGFSQGA LSALLLGYQAQ
Sbjct: 64  FPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQAQ 123

Query: 123 GKVLKEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWLKLPSEELASAF 182
           GKVLKEHPP+K+FVSISG+KF+D SICEVAYKHKIK KSVH IGE DWLKLPS++LAS F
Sbjct: 124 GKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVF 183

Query: 183 HDPFIIRHPQGHTVPRLDEAATRELRCWVDAVLCSDDHAGDGEGKENSIDVMIE 235
           H+P IIRHPQGHTVPRLDE+AT+EL+CWVDA+L S DH GDGE KEN ID + E
Sbjct: 184 HEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDE 237

BLAST of Sgr028431.1 vs. NCBI nr
Match: XP_023539260.1 (family of serine hydrolases 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 415.2 bits (1066), Expect = 3.7e-112
Identity = 196/236 (83.05%), Postives = 209/236 (88.56%), Query Frame = 0

Query: 1   MES-GKRENKMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEG 60
           MES GKR  ++KILCLHGFRTSGSFLRKQL KWDPSIFAHFDMEFPD IFPAGGKSEIEG
Sbjct: 1   MESGGKRAKRLKILCLHGFRTSGSFLRKQLTKWDPSIFAHFDMEFPDAIFPAGGKSEIEG 60

Query: 61  YFPPPYFEWFQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQA 120
           YFPPPYFEWFQFN++FTEYTNLEQ IDYL NYIT  GPF GLLGFSQGA LSALLLGYQA
Sbjct: 61  YFPPPYFEWFQFNEDFTEYTNLEQSIDYLNNYITCNGPFHGLLGFSQGAVLSALLLGYQA 120

Query: 121 QGKVLKEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWLKLPSEELASA 180
           Q KVLKEHPP+K+FVSISG+KF+D SICEVAYKHKIK KSVHLIGE DWLKLPSEELASA
Sbjct: 121 QEKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHLIGEKDWLKLPSEELASA 180

Query: 181 FHDPFIIRHPQGHTVPRLDEAATRELRCWVDAVLCSDDHAGDGEGKENSIDVMIEN 236
           FH+P IIRHPQGHTVPRLDE AT ELRCWVDA++ S DH GDGE KEN    + +N
Sbjct: 181 FHEPLIIRHPQGHTVPRLDEVATEELRCWVDAIVSSHDHVGDGEVKENITQKVADN 236

BLAST of Sgr028431.1 vs. NCBI nr
Match: XP_008443142.1 (PREDICTED: esterase C25G4.2-like [Cucumis melo])

HSP 1 Score: 409.1 bits (1050), Expect = 2.6e-110
Identity = 190/235 (80.85%), Postives = 209/235 (88.94%), Query Frame = 0

Query: 1   MESGKR-ENKMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEG 60
           MESGKR E ++KILCLHGFRT+GSFLRKQL KWDPSIFA F MEFPDGIFPAGGKSEIEG
Sbjct: 1   MESGKRDEERLKILCLHGFRTNGSFLRKQLSKWDPSIFALFHMEFPDGIFPAGGKSEIEG 60

Query: 61  YFPPPYFEWFQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQA 120
           YFPPPYFEWFQFN++FTEY NLE+CIDY+CNYITT GPF GLLGFSQGA LSALLLGYQA
Sbjct: 61  YFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQA 120

Query: 121 QGKVLKEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWLKLPSEELASA 180
           QGKVL+EHPPIK+FVSISG+KF+D SICEVAYKHKIK KSVH IGE DWLKLPS++LAS 
Sbjct: 121 QGKVLREHPPIKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASV 180

Query: 181 FHDPFIIRHPQGHTVPRLDEAATRELRCWVDAVLCSDDHAGDGEGKENSIDVMIE 235
           FH P IIRHPQGHTVPRLDE+AT+EL+CWVD +L S  H GD E KE+ ID + E
Sbjct: 181 FHQPLIIRHPQGHTVPRLDESATKELQCWVDTILSSRGHDGDSEVKESVIDKVDE 235

BLAST of Sgr028431.1 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 3.6e-17
Identity = 56/204 (27.45%), Postives = 94/204 (46.08%), Query Frame = 0

Query: 10  MKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSE------------I 69
           +K+LCLHG+  SG    K++G     +  + ++ FP G   A  +++            +
Sbjct: 5   LKVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLAAL 64

Query: 70  EGYFPPPYFEWFQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGY 129
            G      F WF+       Y + ++ ++ +  Y+  KGPFDGL+GFSQGA + A+L   
Sbjct: 65  GGEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLAQM 124

Query: 130 QAQGK---VLKEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWL--KLP 189
              G+      +HPP K  V + G +       +  Y  K+   S+H+ G  D L     
Sbjct: 125 LQPGQPPNPYVQHPPFKFVVFVGGFRAEKPEF-DHFYNPKLTTPSLHIAGTSDTLVPLAR 184

Query: 190 SEELASAFHDPFIIRHPQGHTVPR 197
           S++L     +  ++ HP  H VP+
Sbjct: 185 SKQLVERCENAHVLLHPGQHIVPQ 207

BLAST of Sgr028431.1 vs. ExPASy Swiss-Prot
Match: Q7QBJ0 (Esterase AGAP003155 OS=Anopheles gambiae OX=7165 GN=AGAP003155 PE=3 SV=3)

HSP 1 Score: 84.7 bits (208), Expect = 1.5e-15
Identity = 61/204 (29.90%), Postives = 92/204 (45.10%), Query Frame = 0

Query: 3   SGKRENKMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEGYFP 62
           SG  E K+K+L LHG+R +    + +LG +   +  + +  F     PA     + G   
Sbjct: 5   SGAGEEKLKVLALHGYRQNADSFKSKLGSFRKMLNKYVEFVFVSAPHPAAPLEAVGGEPD 64

Query: 63  PPYFEWFQFNKEFTEYTNLEQ-----CIDYLCNYITTKGPFD---GLLGFSQGATLSALL 122
           P    W+ FNK+   +    Q       D     +      +   GLLGFSQGA    LL
Sbjct: 65  PNQRSWW-FNKDDRTFKGTNQGGPAYGFDESLRLVERTWQAEGCHGLLGFSQGACFVGLL 124

Query: 123 LGYQAQGKVLKEHPPIKVFVSISGSKFRDASICEV-AYKHKIKAKSVHLIGEMDWL--KL 182
               A+G        +K   ++  S FR  S+  +  Y++K++  S+H+ GE D +  K 
Sbjct: 125 CDLSARGMT-----TMKPQFAVVASGFRSGSLVHLNYYENKVQIPSLHIFGETDEIITKD 184

Query: 183 PSEELASAFHDPFIIRHPQGHTVP 196
            SE LA  F DP ++ HP GH  P
Sbjct: 185 MSEALAETFLDPEVVTHPGGHYFP 202

BLAST of Sgr028431.1 vs. ExPASy Swiss-Prot
Match: Q0C7C4 (Esterase AAEL000016 OS=Aedes aegypti OX=7159 GN=AAEL000016 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.3e-14
Identity = 66/202 (32.67%), Postives = 95/202 (47.03%), Query Frame = 0

Query: 7   ENKMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDG--IFPAGGKSEIEGYFPPP 66
           E+K+KIL LHG+R +G   + +LG +   I  H ++ F     I P    SE  G  P P
Sbjct: 22  ESKLKILALHGYRQNGDGFKSKLGSFRKFIGKHAELVFVTAPHIAPPLPDSE-AGTEPDP 81

Query: 67  YFEWFQFNKEFTEYTNLEQ---CIDY-----LCNYITTKGPFDGLLGFSQGATLSALLLG 126
               + FNK+   +    +    I +     L   +  +    GLLGFSQGA    LL  
Sbjct: 82  AQRSWWFNKDDGTFKGTNKNGPAIGFEDSLKLVEKVWKQEQCCGLLGFSQGACFVGLLCD 141

Query: 127 YQAQGKVLKEHPPIKVFVSISGSKFRDASICEV-AYKHKIKAKSVHLIGEMDWL--KLPS 186
             A+G        IK   ++  S FR  S+  +  Y+ K++  S+H+ GE D +  K  S
Sbjct: 142 LSARGMT-----SIKPEFAVLSSGFRSGSLVHLNCYETKVQIPSLHIYGEADEIIPKEMS 201

Query: 187 EELASAFHDPFIIRHPQGHTVP 196
             LA  F DP I+ HP GH +P
Sbjct: 202 MALADTFTDPQILTHPGGHFLP 217

BLAST of Sgr028431.1 vs. ExPASy Swiss-Prot
Match: O13897 (Uncharacterized hydrolase C22A12.06c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC22A12.06c PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 3.7e-14
Identity = 63/210 (30.00%), Postives = 92/210 (43.81%), Query Frame = 0

Query: 5   KRENKMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDG-IFPAGGKSEIEG---- 64
           K   K KILC+HG+  SG     +L      +    D  FP G I     K E+ G    
Sbjct: 2   KSATKSKILCIHGYAESGELFSVKLRALRERMADSVDFYFPTGPIELDKAKDELNGSGFD 61

Query: 65  -----YFPPPYFE---WFQFNKEFTEYTNLE--QCIDYLCNYITTKGPFDGLLGFSQGAT 124
                +   P      W++ N E+ +   LE  +  +YL +YI   GPFDG+LGFSQG  
Sbjct: 62  ALSTVFSSSPASHRRGWWRIN-EYADTKQLEPTKAFEYLASYIKEHGPFDGILGFSQGTN 121

Query: 125 LSALLLGYQAQGKVLK--EHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMD 184
           L+A L       K  +    PP +  +  SG  FR   +    +  K+   ++HL+G+ D
Sbjct: 122 LAANLAALVTIPKYQEYFSQPPFRFALFFSG-YFRPLLMDGAVHATKLDLPTLHLLGKYD 181

Query: 185 WL--KLPSEELASAFHDPFIIRHPQGHTVP 196
            +     S  L  A  D  ++ HP  H +P
Sbjct: 182 TVLSTETSTTLVRACKDAQVLFHPAAHQIP 209

BLAST of Sgr028431.1 vs. ExPASy Swiss-Prot
Match: Q18169 (Esterase C25G4.2 OS=Caenorhabditis elegans OX=6239 GN=C25G4.2 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 6.3e-14
Identity = 56/202 (27.72%), Postives = 95/202 (47.03%), Query Frame = 0

Query: 9   KMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEGYFPPPYFEW 68
           K++ILCLHG+R      R++ G     + +  + EF +G+        ++ +       W
Sbjct: 6   KLRILCLHGYRQCDQSFRQKTGSTRKLVKSLAEFEFVNGVHSVA----VDEHVDSSRAWW 65

Query: 69  FQFN-------KEFTEY-TNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQAQ 128
           F  N       +E TE     E+ +  +  +I   GPFDGLLGFSQGA++  LL+     
Sbjct: 66  FSNNEAMSFSSRESTEVAVGFEESVAAVVKFIEENGPFDGLLGFSQGASMVHLLIAKAQL 125

Query: 129 GKVLKEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWL--KLPSEELAS 188
           G++  + P I+  +  SG     +    +      +  S+H+ G+ D +  +  SE++A 
Sbjct: 126 GEI--KLPGIRFAIFFSGFLSLSSKHDSLTLLRIKEFPSMHVFGDADEIVARPKSEKMAD 185

Query: 189 AFH-DPFIIRHPQGHTVPRLDE 200
            F  +P  I H  GH VP + +
Sbjct: 186 MFDVEPLRIAHDGGHVVPSMSK 201

BLAST of Sgr028431.1 vs. ExPASy TrEMBL
Match: A0A6J1DLE8 (dihydrofolate reductase-like OS=Momordica charantia OX=3673 GN=LOC111021534 PE=4 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 9.1e-117
Identity = 205/240 (85.42%), Postives = 216/240 (90.00%), Query Frame = 0

Query: 1   MESGKRENKMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEGY 60
           MESGKRENKMKILCLHGFRTSGSFLRKQL KWDPSIFAHFD++FPDG+ PAGGKS+IEGY
Sbjct: 1   MESGKRENKMKILCLHGFRTSGSFLRKQLSKWDPSIFAHFDLDFPDGMCPAGGKSDIEGY 60

Query: 61  FPPPYFEWFQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQAQ 120
           FPPPYFEWFQFN+EFTEYTNLEQCI YLCNYITT GPFDGLLGFSQGATLSALLLGYQAQ
Sbjct: 61  FPPPYFEWFQFNQEFTEYTNLEQCIGYLCNYITTNGPFDGLLGFSQGATLSALLLGYQAQ 120

Query: 121 GKVLKEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWLKLPSEELASAF 180
           GKVLKEHPPIK+ VSISGSKFRD  ICEVAYKHKI+AKSVHLIGE DWLKLPS+ELASAF
Sbjct: 121 GKVLKEHPPIKLLVSISGSKFRDEKICEVAYKHKIEAKSVHLIGEKDWLKLPSQELASAF 180

Query: 181 HDPFIIRHPQGHTVPRLDEAATRELRCWVDAVLCSDDHA-GDGEGKEN----SIDVMIEN 236
           HDP IIRHPQGHTVPRLDEAAT+EL+CWVDAVL   DH   D E KEN     +D  IEN
Sbjct: 181 HDPLIIRHPQGHTVPRLDEAATKELQCWVDAVLSGHDHVIEDEEVKENYVIDKMDEKIEN 240

BLAST of Sgr028431.1 vs. ExPASy TrEMBL
Match: A0A0A0LH35 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G813300 PE=4 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.6e-113
Identity = 192/234 (82.05%), Postives = 213/234 (91.03%), Query Frame = 0

Query: 3   SGKRENK--MKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEGY 62
           S KR++K  +KILCLHGFRT+GSFLRKQ+ KWDPSIFAHFDMEFPDGIFPAGGKSEIEGY
Sbjct: 4   SSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEIEGY 63

Query: 63  FPPPYFEWFQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQAQ 122
           FPPPYFEWFQFN++FTEY NLE+CIDY+CNYITT GPF GLLGFSQGA LSALLLGYQAQ
Sbjct: 64  FPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQAQ 123

Query: 123 GKVLKEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWLKLPSEELASAF 182
           GKVLKEHPP+K+FVSISG+KF+D SICEVAYKHKIK KSVH IGE DWLKLPS++LAS F
Sbjct: 124 GKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVF 183

Query: 183 HDPFIIRHPQGHTVPRLDEAATRELRCWVDAVLCSDDHAGDGEGKENSIDVMIE 235
           H+P IIRHPQGHTVPRLDE+AT+EL+CWVDA+L S DH GDGE KEN ID + E
Sbjct: 184 HEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDE 237

BLAST of Sgr028431.1 vs. ExPASy TrEMBL
Match: A0A1S3B6W5 (esterase C25G4.2-like OS=Cucumis melo OX=3656 GN=LOC103486820 PE=4 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.3e-110
Identity = 190/235 (80.85%), Postives = 209/235 (88.94%), Query Frame = 0

Query: 1   MESGKR-ENKMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEG 60
           MESGKR E ++KILCLHGFRT+GSFLRKQL KWDPSIFA F MEFPDGIFPAGGKSEIEG
Sbjct: 1   MESGKRDEERLKILCLHGFRTNGSFLRKQLSKWDPSIFALFHMEFPDGIFPAGGKSEIEG 60

Query: 61  YFPPPYFEWFQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQA 120
           YFPPPYFEWFQFN++FTEY NLE+CIDY+CNYITT GPF GLLGFSQGA LSALLLGYQA
Sbjct: 61  YFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQA 120

Query: 121 QGKVLKEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWLKLPSEELASA 180
           QGKVL+EHPPIK+FVSISG+KF+D SICEVAYKHKIK KSVH IGE DWLKLPS++LAS 
Sbjct: 121 QGKVLREHPPIKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASV 180

Query: 181 FHDPFIIRHPQGHTVPRLDEAATRELRCWVDAVLCSDDHAGDGEGKENSIDVMIE 235
           FH P IIRHPQGHTVPRLDE+AT+EL+CWVD +L S  H GD E KE+ ID + E
Sbjct: 181 FHQPLIIRHPQGHTVPRLDESATKELQCWVDTILSSRGHDGDSEVKESVIDKVDE 235

BLAST of Sgr028431.1 vs. ExPASy TrEMBL
Match: A0A2P2J1W4 (Dihydrofolate reductase-like OS=Rhizophora mucronata OX=61149 PE=4 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.3e-102
Identity = 176/224 (78.57%), Postives = 196/224 (87.50%), Query Frame = 0

Query: 5   KRENKMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEGYFPPP 64
           + E KMKILCLHGFRTSGSFL+KQ+ KWDPSI AHFDM+FPDG FPAGGKS+IEG FPPP
Sbjct: 4   QEEKKMKILCLHGFRTSGSFLKKQISKWDPSILAHFDMDFPDGKFPAGGKSDIEGIFPPP 63

Query: 65  YFEWFQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQAQGKVL 124
           YFEWFQFNKEFTEYTNLE+CI YLC YIT KGPFDGLLGFSQGATLSALLLGYQAQGKVL
Sbjct: 64  YFEWFQFNKEFTEYTNLEECISYLCEYITNKGPFDGLLGFSQGATLSALLLGYQAQGKVL 123

Query: 125 KEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWLKLPSEELASAFHDPF 184
           KEHPPIK+FVSISGSKFRD +IC+VAYK+ I+ KSVH IGE DWLKLPSE+LA+AF +P 
Sbjct: 124 KEHPPIKIFVSISGSKFRDPTICDVAYKNTIRVKSVHFIGEKDWLKLPSEDLATAFDNPL 183

Query: 185 IIRHPQGHTVPRLDEAATRELRCWVDAVL-CSDDHAGDGEGKEN 228
           IIRHPQGHTVPRLDEA+T +LR W   +L C++    D +G EN
Sbjct: 184 IIRHPQGHTVPRLDEASTEQLRAWAAEILKCNNTIMNDKQGLEN 227

BLAST of Sgr028431.1 vs. ExPASy TrEMBL
Match: A0A6M2EP39 (FSH1 domain-containing protein OS=Populus davidiana OX=266767 PE=4 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.9e-99
Identity = 169/226 (74.78%), Postives = 191/226 (84.51%), Query Frame = 0

Query: 1   MESGKRENKMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEGY 60
           M + + E KMKILCLHGFRTSGSFL++Q+ KWDPSIF+ FD++FPDG+FPAGGKSEIEG 
Sbjct: 1   MATSQDEKKMKILCLHGFRTSGSFLQRQISKWDPSIFSQFDLDFPDGVFPAGGKSEIEGI 60

Query: 61  FPPPYFEWFQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQAQ 120
           FPPPYFEWFQF+K+FTEYTNLE+CI YLC YITT+GPFDG LGFSQGATLSALLLGYQAQ
Sbjct: 61  FPPPYFEWFQFDKDFTEYTNLEECISYLCEYITTRGPFDGFLGFSQGATLSALLLGYQAQ 120

Query: 121 GKVLKEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWLKLPSEELASAF 180
           GKVLK+HPP K+FVS+SGSKFRD SIC+VAYK  IK KSVH IG  DWLK PSEELA+AF
Sbjct: 121 GKVLKDHPPFKLFVSVSGSKFRDPSICDVAYKDTIKVKSVHFIGAKDWLKEPSEELATAF 180

Query: 181 HDPFIIRHPQGHTVPRLDEAATRELRCWVDAVLCSDDHAGDGEGKE 227
             P IIRHPQGHTVPRLDEAAT +LR W   +L  ++    GE  E
Sbjct: 181 DSPLIIRHPQGHTVPRLDEAATEQLRAWTTEILSHNNKILKGENHE 226

BLAST of Sgr028431.1 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 221.1 bits (562), Expect = 9.5e-58
Identity = 103/222 (46.40%), Postives = 146/222 (65.77%), Query Frame = 0

Query: 9   KMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEGYFPPPYFEW 68
           K + LCLHGFRTSG  ++ QL KW  S+    D+ F D  FP  GKS++EG F PPY+EW
Sbjct: 11  KPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVEGIFDPPYYEW 70

Query: 69  FQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQAQGKVLKEHP 128
           FQFNKEFTEYTN E+C++YL + +   GPFDGL+GFSQGA LS  L G QA+G   ++ P
Sbjct: 71  FQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQAKGIAFQKVP 130

Query: 129 PIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIGEMDWLKLPSEELASAFHDPFIIRH 188
            IK  + I G+K + A + E AY   ++  S+H +GE D+LK    +L  ++ +P ++ H
Sbjct: 131 KIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLGETDFLKPYGTQLIESYKNPVVVHH 190

Query: 189 PQGHTVPRLDEAATRELRCWVDAV--LCSDDHAGDGEGKENS 229
           P+GHTVPRLDE +  ++  ++D +  L  ++   D  G+EN+
Sbjct: 191 PKGHTVPRLDEKSLEKVTAFIDTLEHLVMEE---DKNGEENT 229

BLAST of Sgr028431.1 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 199.5 bits (506), Expect = 3.0e-51
Identity = 92/207 (44.44%), Postives = 129/207 (62.32%), Query Frame = 0

Query: 11  KILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEGYFPPPYFEWFQ 70
           +ILCLHGFRTSG  L+  +GKW  +I    D++F D  FPA GKS++E +F PPY+EW+Q
Sbjct: 31  RILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSDVERFFDPPYYEWYQ 90

Query: 71  FNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQAQGKVLKEHPPI 130
            NK F EY N E+C+ Y+ +Y+   GPFDGLLGFSQGA L+A + G Q QG  L + P +
Sbjct: 91  ANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQEQGSALTKVPKV 150

Query: 131 KVFVSISGSK-----FRDASICEVAYKHKIKAKSVHLIGEMDWLKLPSEELASAFHDPFI 190
           K  V ISG+K     F +      A+   ++  S+H IGE D+LK+  E L  +F +P +
Sbjct: 151 KFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFIGERDFLKIEGEVLVESFVEPVV 210

Query: 191 IRHPQGHTVPRLDEAATRELRCWVDAV 213
           I H  GH +P+LD  A   +  +  ++
Sbjct: 211 IHHSGGHIIPKLDTKAEETMLSFFQSI 237

BLAST of Sgr028431.1 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 175.6 bits (444), Expect = 4.6e-44
Identity = 80/156 (51.28%), Postives = 105/156 (67.31%), Query Frame = 0

Query: 9   KMKILCLHGFRTSGSFLRKQLGKWDPSIFAHFDMEFPDGIFPAGGKSEIEGYFPPPYFEW 68
           K + LCLHGFRTSG  ++ QL KW  S+    D+ F D  FP  GKS++EG F PPY+EW
Sbjct: 11  KPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVEGIFDPPYYEW 70

Query: 69  FQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLLGFSQGATLSALLLGYQAQGKVLKEHP 128
           FQFNKEFTEYTN E+C++YL + +   GPFDGL+GFSQGA LS  L G QA+G   ++ P
Sbjct: 71  FQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQAKGIAFQKVP 130

Query: 129 PIKVFVSISGSKFRDASICEVAYKHKIKAKSVHLIG 165
            IK  + I G+K + A + E AY   ++  S+H +G
Sbjct: 131 KIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLG 166

BLAST of Sgr028431.1 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 71.2 bits (173), Expect = 1.2e-12
Identity = 62/220 (28.18%), Postives = 92/220 (41.82%), Query Frame = 0

Query: 9   KMKILCLHGFRTSGSFLRKQLGKWDPS---------IFAHFDMEF-------PDGIFPAG 68
           K++ILCLHGFR + S  + + G              I A  +++F       P G+    
Sbjct: 353 KLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCNKK 412

Query: 69  GKSEIEGYFPPP----------YFEWFQFNKEFTEYTNLEQCIDYLCNYITTKGPFDGLL 128
               +   F  P           F+  Q+    T+    ++ + YL      KGPFDG+L
Sbjct: 413 FAWLVSSDFDKPSETGWTVAQCQFDPLQYQ---TQTEGFDKSLTYLKTAFEEKGPFDGIL 472

Query: 129 GFSQGATLSALLLGYQAQGKVLKEHPPIKVFVSISGSKFRDASICEVAYKHKIKAKSVHL 188
           GFSQGA ++A + G Q Q   L      +  V  SG  F    + E+  K  IK  S+H+
Sbjct: 473 GFSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHI 532

Query: 189 IG-----EMDWLKLPSEELASAFHD--PFIIRHPQGHTVP 196
            G     +   +   S +LA  F D    I+ H  GH +P
Sbjct: 533 FGSQPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154224.11.9e-11685.42dihydrofolate reductase-like [Momordica charantia][more]
KAG6596208.12.5e-11382.63Esterase-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_004136600.13.3e-11382.05esterase OVCA2 [Cucumis sativus] >KGN59361.1 hypothetical protein Csa_001052 [Cu... [more]
XP_023539260.13.7e-11283.05family of serine hydrolases 2-like [Cucurbita pepo subsp. pepo][more]
XP_008443142.12.6e-11080.85PREDICTED: esterase C25G4.2-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
P365913.6e-1727.45Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O... [more]
Q7QBJ01.5e-1529.90Esterase AGAP003155 OS=Anopheles gambiae OX=7165 GN=AGAP003155 PE=3 SV=3[more]
Q0C7C41.3e-1432.67Esterase AAEL000016 OS=Aedes aegypti OX=7159 GN=AAEL000016 PE=3 SV=1[more]
O138973.7e-1430.00Uncharacterized hydrolase C22A12.06c OS=Schizosaccharomyces pombe (strain 972 / ... [more]
Q181696.3e-1427.72Esterase C25G4.2 OS=Caenorhabditis elegans OX=6239 GN=C25G4.2 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DLE89.1e-11785.42dihydrofolate reductase-like OS=Momordica charantia OX=3673 GN=LOC111021534 PE=4... [more]
A0A0A0LH351.6e-11382.05FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G813300 PE=4 S... [more]
A0A1S3B6W51.3e-11080.85esterase C25G4.2-like OS=Cucumis melo OX=3656 GN=LOC103486820 PE=4 SV=1[more]
A0A2P2J1W41.3e-10278.57Dihydrofolate reductase-like OS=Rhizophora mucronata OX=61149 PE=4 SV=1[more]
A0A6M2EP393.9e-9974.78FSH1 domain-containing protein OS=Populus davidiana OX=266767 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G24380.19.5e-5846.40INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and deri... [more]
AT5G65400.13.0e-5144.44alpha/beta-Hydrolases superfamily protein [more]
AT4G24380.24.6e-4451.28CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidop... [more]
AT1G09280.11.2e-1228.18CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolas... [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005645Serine hydrolase FSHPFAMPF03959FSH1coord: 8..200
e-value: 5.1E-46
score: 157.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 6..213
e-value: 2.0E-47
score: 163.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 7..195
NoneNo IPR availablePANTHERPTHR48070:SF10OS07G0687100 PROTEINcoord: 5..219
NoneNo IPR availablePANTHERPTHR48070ESTERASE OVCA2coord: 5..219

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Sgr028431Sgr028431gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sgr028431.1.exon6Sgr028431.1.exon6exon
Sgr028431.1.exon5Sgr028431.1.exon5exon
Sgr028431.1.exon4Sgr028431.1.exon4exon
Sgr028431.1.exon3Sgr028431.1.exon3exon
Sgr028431.1.exon2Sgr028431.1.exon2exon
Sgr028431.1.exon1Sgr028431.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Sgr028431.1cds.Sgr028431.1_6CDS
cds.Sgr028431.1cds.Sgr028431.1_5CDS
cds.Sgr028431.1cds.Sgr028431.1_4CDS
cds.Sgr028431.1cds.Sgr028431.1_3CDS
cds.Sgr028431.1cds.Sgr028431.1_2CDS
cds.Sgr028431.1cds.Sgr028431.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Sgr028431.1Sgr028431.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0016787 hydrolase activity