Sgr028190.1 (mRNA) Monk fruit (Qingpiguo) v1

BLAST of Sgr028190.1 vs. NCBI nr
Match: XP_022139568.1 (probable ubiquitin-conjugating enzyme E2 26 isoform X2 [Momordica charantia])

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 548/712 (76.97%), Postives = 608/712 (85.39%), Query Frame = 0

Query: 107 IISLDVIDVDKDEACDNAIFIHDNFDTNDKGKGIYHPSDHQLKETLASRSFSPDVEILES 166
           I S++VIDVDKDE  +N I+IHD  + NDKGKGIYH SDH+L+E +AS S+SPDVEILE 
Sbjct: 24  IQSVEVIDVDKDEDFNNVIYIHDGVENNDKGKGIYHNSDHELQEMMASPSYSPDVEILEG 83

Query: 167 VNEVKPSFPSASHNLIDLDNHVSDLSNEDDEWVPFDEFMDVDEYSALQAHFDHMDIPPDI 226
            NEV+ +FPSA HN+IDLDNH SD SN  DEW+PFDE MDVDEYSALQAHFDHMDIPPDI
Sbjct: 84  GNEVESAFPSAQHNVIDLDNHYSDSSN-GDEWIPFDEIMDVDEYSALQAHFDHMDIPPDI 143

Query: 227 EAPIPWLPPHGENRYKSYFEISSSFAEPQIEPGSVNQQGKSPAQFKLKATLANSLNMQAE 286
           EAPIPWL PHG+NR+KS F  SSSFAE QI PGSVNQ G SPA  KLKATLANSLN+QAE
Sbjct: 144 EAPIPWL-PHGQNRHKSIFGTSSSFAESQINPGSVNQLGNSPAHAKLKATLANSLNLQAE 203

Query: 287 IDGVNHPPEGIEP---LPSQDVLPTKKSAASRYRDKSVSPHDVSYINSLLSVEAAKSRWF 346
           ID VNHPPEG EP     SQDVLP KKS ASRYRDKS  PH+V  INSLL  EAAK+RWF
Sbjct: 204 IDIVNHPPEGTEPFTSFTSQDVLPNKKSVASRYRDKSGLPHEV--INSLLGAEAAKTRWF 263

Query: 347 LDPFKRKKKLYGSNNISINQTDAMKLH-NGGETSFDTSDSVKKQVGSHIISHSNFPAYMD 406
            DPFKRKKKL+GSNNISINQ+D+ KLH NGGETS DTS++VKKQ  S+ +S  NFPAYMD
Sbjct: 264 SDPFKRKKKLHGSNNISINQSDSRKLHNNGGETSSDTSETVKKQFSSNNMSSFNFPAYMD 323

Query: 407 LFNYPPEAGPSIPHWPDYAKWKTKEPFSTPHNAPDFYGPF------NPLHPPTLGEVVDM 466
              YPP AG S+PHWPDYAK KTKE    P +  D+YGPF      +PLHPPTLGEVVDM
Sbjct: 324 NIYYPPGAGASLPHWPDYAKMKTKELVLNPPSVSDYYGPFDHLIPPHPLHPPTLGEVVDM 383

Query: 467 PWIQGSTQNLANLAPGNSTHEIITCKDMDELLRNFERFKQFDTVEDHSDHRYTSSGFTMK 526
           PWIQGST++ ANLAP NST E I+  D+DE++R FE+FK+FDTV+DHSDH YTS GFTMK
Sbjct: 384 PWIQGSTRDPANLAPDNSTLETISIADLDEIMRKFEQFKRFDTVDDHSDHHYTSKGFTMK 443

Query: 527 QLPRKWTKRIQEEWKILEKDLPETIFVRTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFF 586
           QLP+KWTKRIQEEWKILEKDLP+TIFVR YESRMDL+RAVIIGAEGTPYHDGLFFFDIFF
Sbjct: 444 QLPKKWTKRIQEEWKILEKDLPDTIFVRAYESRMDLLRAVIIGAEGTPYHDGLFFFDIFF 503

Query: 587 PPSYPDVPPQVYYHSGGLRLNPNLYNCGKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSI 646
           P  YPDVPP VYYHSGGLRLNPNLYNCGKVCLSLLNTWSGSGNERW+PGMSTMLQVLVSI
Sbjct: 504 PMGYPDVPPLVYYHSGGLRLNPNLYNCGKVCLSLLNTWSGSGNERWIPGMSTMLQVLVSI 563

Query: 647 QGLILNTKPYFNEPGYAYQSGSVAGENKSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVR 706
           QGLILNTKPYFNEPG+A Q+GSVAGEN+SQ YNEE Y+LS+KTMVYNIRRPPKHFEDFV+
Sbjct: 564 QGLILNTKPYFNEPGFANQNGSVAGENRSQQYNEETYLLSMKTMVYNIRRPPKHFEDFVK 623

Query: 707 GHFCKRAHDILVACKAYMDGAQVGSLVKDGVQDLDLGDKSCSNHFKQSLYQHLRMLVETF 766
           GHF KRAHDILV+CKAYMDGAQVGSLVK GVQDLDL DK CSNHFKQSL Q+L MLV+TF
Sbjct: 624 GHFFKRAHDILVSCKAYMDGAQVGSLVKGGVQDLDLSDKCCSNHFKQSLSQYLSMLVKTF 683

Query: 767 TQLGVSDCEKFLPPLPSPSPLVPNARKRSGRHTKVQLESLEGDGA-EKLVLG 808
            ++GV DCEKFL  LP PS  V +A+KRSGRH+++++ESL GDGA EKLVLG
Sbjct: 684 QRMGVGDCEKFL--LPPPSLPVSSAKKRSGRHSELKIESLNGDGATEKLVLG 729

BLAST of Sgr028190.1 vs. NCBI nr
Match: XP_022139567.1 (probable ubiquitin-conjugating enzyme E2 26 isoform X1 [Momordica charantia])

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 568/808 (70.30%), Postives = 635/808 (78.59%), Query Frame = 0

Query: 13  DHQMEPP--PNESQNSKSSISFFFFPILLSFLCFFLNFLFLFLSLLLCFSNLSDCFSAMT 72
           DHQMEPP  PN SQNSK+ +                                    S   
Sbjct: 8   DHQMEPPPSPNSSQNSKTKL-----------------------------------LSGTG 67

Query: 73  SGKEKNLASVLDTLNTLKIDLNMDRVTESDISSAMSIISLDVIDVDKDEACDNAIFIHDN 132
           SG  K           L+    ++R  +  +     I S++VIDVDKDE  +N I+IHD 
Sbjct: 68  SGSMKP--------QVLRHSPLINRPPK--MLKNKGIQSVEVIDVDKDEDFNNVIYIHDG 127

Query: 133 FDTNDKGKGIYHPSDHQLKETLASRSFSPDVEILESVNEVKPSFPSASHNLIDLDNHVSD 192
            + NDKGKGIYH SDH+L+E +AS S+SPDVEILE  NEV+ +FPSA HN+IDLDNH SD
Sbjct: 128 VENNDKGKGIYHNSDHELQEMMASPSYSPDVEILEGGNEVESAFPSAQHNVIDLDNHYSD 187

Query: 193 LSNEDDEWVPFDEFMDVDEYSALQAHFDHMDIPPDIEAPIPWLPPHGENRYKSYFEISSS 252
            SN  DEW+PFDE MDVDEYSALQAHFDHMDIPPDIEAPIPWL PHG+NR+KS F  SSS
Sbjct: 188 SSN-GDEWIPFDEIMDVDEYSALQAHFDHMDIPPDIEAPIPWL-PHGQNRHKSIFGTSSS 247

Query: 253 FAEPQIEPGSVNQQGKSPAQFKLKATLANSLNMQAEIDGVNHPPEGIEP---LPSQDVLP 312
           FAE QI PGSVNQ G SPA  KLKATLANSLN+QAEID VNHPPEG EP     SQDVLP
Sbjct: 248 FAESQINPGSVNQLGNSPAHAKLKATLANSLNLQAEIDIVNHPPEGTEPFTSFTSQDVLP 307

Query: 313 TKKSAASRYRDKSVSPHDVSYINSLLSVEAAKSRWFLDPFKRKKKLYGSNNISINQTDAM 372
            KKS ASRYRDKS  PH+V  INSLL  EAAK+RWF DPFKRKKKL+GSNNISINQ+D+ 
Sbjct: 308 NKKSVASRYRDKSGLPHEV--INSLLGAEAAKTRWFSDPFKRKKKLHGSNNISINQSDSR 367

Query: 373 KLH-NGGETSFDTSDSVKKQVGSHIISHSNFPAYMDLFNYPPEAGPSIPHWPDYAKWKTK 432
           KLH NGGETS DTS++VKKQ  S+ +S  NFPAYMD   YPP AG S+PHWPDYAK KTK
Sbjct: 368 KLHNNGGETSSDTSETVKKQFSSNNMSSFNFPAYMDNIYYPPGAGASLPHWPDYAKMKTK 427

Query: 433 EPFSTPHNAPDFYGPF------NPLHPPTLGEVVDMPWIQGSTQNLANLAPGNSTHEIIT 492
           E    P +  D+YGPF      +PLHPPTLGEVVDMPWIQGST++ ANLAP NST E I+
Sbjct: 428 ELVLNPPSVSDYYGPFDHLIPPHPLHPPTLGEVVDMPWIQGSTRDPANLAPDNSTLETIS 487

Query: 493 CKDMDELLRNFERFKQFDTVEDHSDHRYTSSGFTMKQLPRKWTKRIQEEWKILEKDLPET 552
             D+DE++R FE+FK+FDTV+DHSDH YTS GFTMKQLP+KWTKRIQEEWKILEKDLP+T
Sbjct: 488 IADLDEIMRKFEQFKRFDTVDDHSDHHYTSKGFTMKQLPKKWTKRIQEEWKILEKDLPDT 547

Query: 553 IFVRTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNL 612
           IFVR YESRMDL+RAVIIGAEGTPYHDGLFFFDIFFP  YPDVPP VYYHSGGLRLNPNL
Sbjct: 548 IFVRAYESRMDLLRAVIIGAEGTPYHDGLFFFDIFFPMGYPDVPPLVYYHSGGLRLNPNL 607

Query: 613 YNCGKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVA 672
           YNCGKVCLSLLNTWSGSGNERW+PGMSTMLQVLVSIQGLILNTKPYFNEPG+A Q+GSVA
Sbjct: 608 YNCGKVCLSLLNTWSGSGNERWIPGMSTMLQVLVSIQGLILNTKPYFNEPGFANQNGSVA 667

Query: 673 GENKSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVG 732
           GEN+SQ YNEE Y+LS+KTMVYNIRRPPKHFEDFV+GHF KRAHDILV+CKAYMDGAQVG
Sbjct: 668 GENRSQQYNEETYLLSMKTMVYNIRRPPKHFEDFVKGHFFKRAHDILVSCKAYMDGAQVG 727

Query: 733 SLVKDGVQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLGVSDCEKFLPPLPSPSPLVPN 792
           SLVK GVQDLDL DK CSNHFKQSL Q+L MLV+TF ++GV DCEKFL  LP PS  V +
Sbjct: 728 SLVKGGVQDLDLSDKCCSNHFKQSLSQYLSMLVKTFQRMGVGDCEKFL--LPPPSLPVSS 764

Query: 793 ARKRSGRHTKVQLESLEGDGA-EKLVLG 808
           A+KRSGRH+++++ESL GDGA EKLVLG
Sbjct: 788 AKKRSGRHSELKIESLNGDGATEKLVLG 764

BLAST of Sgr028190.1 vs. NCBI nr
Match: XP_022139569.1 (uncharacterized protein LOC111010440 isoform X3 [Momordica charantia])

HSP 1 Score: 1029.6 bits (2661), Expect = 1.4e-296
Identity = 538/805 (66.83%), Postives = 604/805 (75.03%), Query Frame = 0

Query: 13  DHQMEPP--PNESQNSKSSISFFFFPILLSFLCFFLNFLFLFLSLLLCFSNLSDCFSAMT 72
           DHQMEPP  PN SQNSK+ +                                    S   
Sbjct: 8   DHQMEPPPSPNSSQNSKTKL-----------------------------------LSGTG 67

Query: 73  SGKEKNLASVLDTLNTLKIDLNMDRVTESDISSAMSIISLDVIDVDKDEACDNAIFIHDN 132
           SG  K           L+    ++R  +  +     I S++VIDVDKDE  +N I+IHD 
Sbjct: 68  SGSMKP--------QVLRHSPLINRPPK--MLKNKGIQSVEVIDVDKDEDFNNVIYIHDG 127

Query: 133 FDTNDKGKGIYHPSDHQLKETLASRSFSPDVEILESVNEVKPSFPSASHNLIDLDNHVSD 192
            + NDKGKGIYH SDH+L+E +AS S+SPDVEILE  NEV+ +FPSA HN+IDLDNH SD
Sbjct: 128 VENNDKGKGIYHNSDHELQEMMASPSYSPDVEILEGGNEVESAFPSAQHNVIDLDNHYSD 187

Query: 193 LSNEDDEWVPFDEFMDVDEYSALQAHFDHMDIPPDIEAPIPWLPPHGENRYKSYFEISSS 252
            SN  DEW+PFDE MDVDEYSALQAHFDHMDIPPDIEAPIPWL PHG+NR+KS F  SSS
Sbjct: 188 SSN-GDEWIPFDEIMDVDEYSALQAHFDHMDIPPDIEAPIPWL-PHGQNRHKSIFGTSSS 247

Query: 253 FAEPQIEPGSVNQQGKSPAQFKLKATLANSLNMQAEIDGVNHPPEGIEPLPSQDVLPTKK 312
           FAE QI PGSVNQ G SPA  KLKATLANSLN+QAEID VNHPPE               
Sbjct: 248 FAESQINPGSVNQLGNSPAHAKLKATLANSLNLQAEIDIVNHPPE--------------- 307

Query: 313 SAASRYRDKSVSPHDVSYINSLLSVEAAKSRWFLDPFKRKKKLYGSNNISINQTDAMKLH 372
                                      AK+RWF DPFKRKKKL+GSNNISINQ+D+ KLH
Sbjct: 308 ---------------------------AKTRWFSDPFKRKKKLHGSNNISINQSDSRKLH 367

Query: 373 -NGGETSFDTSDSVKKQVGSHIISHSNFPAYMDLFNYPPEAGPSIPHWPDYAKWKTKEPF 432
            NGGETS DTS++VKKQ  S+ +S  NFPAYMD   YPP AG S+PHWPDYAK KTKE  
Sbjct: 368 NNGGETSSDTSETVKKQFSSNNMSSFNFPAYMDNIYYPPGAGASLPHWPDYAKMKTKELV 427

Query: 433 STPHNAPDFYGPF------NPLHPPTLGEVVDMPWIQGSTQNLANLAPGNSTHEIITCKD 492
             P +  D+YGPF      +PLHPPTLGEVVDMPWIQGST++ ANLAP NST E I+  D
Sbjct: 428 LNPPSVSDYYGPFDHLIPPHPLHPPTLGEVVDMPWIQGSTRDPANLAPDNSTLETISIAD 487

Query: 493 MDELLRNFERFKQFDTVEDHSDHRYTSSGFTMKQLPRKWTKRIQEEWKILEKDLPETIFV 552
           +DE++R FE+FK+FDTV+DHSDH YTS GFTMKQLP+KWTKRIQEEWKILEKDLP+TIFV
Sbjct: 488 LDEIMRKFEQFKRFDTVDDHSDHHYTSKGFTMKQLPKKWTKRIQEEWKILEKDLPDTIFV 547

Query: 553 RTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNLYNC 612
           R YESRMDL+RAVIIGAEGTPYHDGLFFFDIFFP  YPDVPP VYYHSGGLRLNPNLYNC
Sbjct: 548 RAYESRMDLLRAVIIGAEGTPYHDGLFFFDIFFPMGYPDVPPLVYYHSGGLRLNPNLYNC 607

Query: 613 GKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVAGEN 672
           GKVCLSLLNTWSGSGNERW+PGMSTMLQVLVSIQGLILNTKPYFNEPG+A Q+GSVAGEN
Sbjct: 608 GKVCLSLLNTWSGSGNERWIPGMSTMLQVLVSIQGLILNTKPYFNEPGFANQNGSVAGEN 667

Query: 673 KSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVGSLV 732
           +SQ YNEE Y+LS+KTMVYNIRRPPKHFEDFV+GHF KRAHDILV+CKAYMDGAQVGSLV
Sbjct: 668 RSQQYNEETYLLSMKTMVYNIRRPPKHFEDFVKGHFFKRAHDILVSCKAYMDGAQVGSLV 721

Query: 733 KDGVQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLGVSDCEKFLPPLPSPSPLVPNARK 792
           K GVQDLDL DK CSNHFKQSL Q+L MLV+TF ++GV DCEKFL  LP PS  V +A+K
Sbjct: 728 KGGVQDLDLSDKCCSNHFKQSLSQYLSMLVKTFQRMGVGDCEKFL--LPPPSLPVSSAKK 721

Query: 793 RSGRHTKVQLESLEGDGA-EKLVLG 808
           RSGRH+++++ESL GDGA EKLVLG
Sbjct: 788 RSGRHSELKIESLNGDGATEKLVLG 721

BLAST of Sgr028190.1 vs. NCBI nr
Match: XP_022139570.1 (probable ubiquitin-conjugating enzyme E2 25 isoform X4 [Momordica charantia])

HSP 1 Score: 1015.0 bits (2623), Expect = 3.6e-292
Identity = 533/805 (66.21%), Postives = 598/805 (74.29%), Query Frame = 0

Query: 13  DHQMEPP--PNESQNSKSSISFFFFPILLSFLCFFLNFLFLFLSLLLCFSNLSDCFSAMT 72
           DHQMEPP  PN SQNSK+ +                                    S   
Sbjct: 8   DHQMEPPPSPNSSQNSKTKL-----------------------------------LSGTG 67

Query: 73  SGKEKNLASVLDTLNTLKIDLNMDRVTESDISSAMSIISLDVIDVDKDEACDNAIFIHDN 132
           SG  K           L+    ++R  +  +     I S++VIDVDKDE  +N I+IHD 
Sbjct: 68  SGSMKP--------QVLRHSPLINRPPK--MLKNKGIQSVEVIDVDKDEDFNNVIYIHDG 127

Query: 133 FDTNDKGKGIYHPSDHQLKETLASRSFSPDVEILESVNEVKPSFPSASHNLIDLDNHVSD 192
            + NDKGKGIYH SDH+L+E +AS S+SPDVEILE  NEV+ +FPSA HN+IDLDNH SD
Sbjct: 128 VENNDKGKGIYHNSDHELQEMMASPSYSPDVEILEGGNEVESAFPSAQHNVIDLDNHYSD 187

Query: 193 LSNEDDEWVPFDEFMDVDEYSALQAHFDHMDIPPDIEAPIPWLPPHGENRYKSYFEISSS 252
            SN  DEW+PFDE MDVDEYSALQAHFDHMDIPPDIEAPIPWL PHG+NR+KS F  SSS
Sbjct: 188 SSN-GDEWIPFDEIMDVDEYSALQAHFDHMDIPPDIEAPIPWL-PHGQNRHKSIFGTSSS 247

Query: 253 FAEPQIEPGSVNQQGKSPAQFKLKATLANSLNMQAEIDGVNHPPEGIEPLPSQDVLPTKK 312
           FAE QI PGSVNQ G SPA  KLKATLANSLN+QAEID VNHPPE               
Sbjct: 248 FAESQINPGSVNQLGNSPAHAKLKATLANSLNLQAEIDIVNHPPE--------------- 307

Query: 313 SAASRYRDKSVSPHDVSYINSLLSVEAAKSRWFLDPFKRKKKLYGSNNISINQTDAMKLH 372
                                             DPFKRKKKL+GSNNISINQ+D+ KLH
Sbjct: 308 ----------------------------------DPFKRKKKLHGSNNISINQSDSRKLH 367

Query: 373 -NGGETSFDTSDSVKKQVGSHIISHSNFPAYMDLFNYPPEAGPSIPHWPDYAKWKTKEPF 432
            NGGETS DTS++VKKQ  S+ +S  NFPAYMD   YPP AG S+PHWPDYAK KTKE  
Sbjct: 368 NNGGETSSDTSETVKKQFSSNNMSSFNFPAYMDNIYYPPGAGASLPHWPDYAKMKTKELV 427

Query: 433 STPHNAPDFYGPF------NPLHPPTLGEVVDMPWIQGSTQNLANLAPGNSTHEIITCKD 492
             P +  D+YGPF      +PLHPPTLGEVVDMPWIQGST++ ANLAP NST E I+  D
Sbjct: 428 LNPPSVSDYYGPFDHLIPPHPLHPPTLGEVVDMPWIQGSTRDPANLAPDNSTLETISIAD 487

Query: 493 MDELLRNFERFKQFDTVEDHSDHRYTSSGFTMKQLPRKWTKRIQEEWKILEKDLPETIFV 552
           +DE++R FE+FK+FDTV+DHSDH YTS GFTMKQLP+KWTKRIQEEWKILEKDLP+TIFV
Sbjct: 488 LDEIMRKFEQFKRFDTVDDHSDHHYTSKGFTMKQLPKKWTKRIQEEWKILEKDLPDTIFV 547

Query: 553 RTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNLYNC 612
           R YESRMDL+RAVIIGAEGTPYHDGLFFFDIFFP  YPDVPP VYYHSGGLRLNPNLYNC
Sbjct: 548 RAYESRMDLLRAVIIGAEGTPYHDGLFFFDIFFPMGYPDVPPLVYYHSGGLRLNPNLYNC 607

Query: 613 GKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVAGEN 672
           GKVCLSLLNTWSGSGNERW+PGMSTMLQVLVSIQGLILNTKPYFNEPG+A Q+GSVAGEN
Sbjct: 608 GKVCLSLLNTWSGSGNERWIPGMSTMLQVLVSIQGLILNTKPYFNEPGFANQNGSVAGEN 667

Query: 673 KSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVGSLV 732
           +SQ YNEE Y+LS+KTMVYNIRRPPKHFEDFV+GHF KRAHDILV+CKAYMDGAQVGSLV
Sbjct: 668 RSQQYNEETYLLSMKTMVYNIRRPPKHFEDFVKGHFFKRAHDILVSCKAYMDGAQVGSLV 714

Query: 733 KDGVQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLGVSDCEKFLPPLPSPSPLVPNARK 792
           K GVQDLDL DK CSNHFKQSL Q+L MLV+TF ++GV DCEKFL  LP PS  V +A+K
Sbjct: 728 KGGVQDLDLSDKCCSNHFKQSLSQYLSMLVKTFQRMGVGDCEKFL--LPPPSLPVSSAKK 714

Query: 793 RSGRHTKVQLESLEGDGA-EKLVLG 808
           RSGRH+++++ESL GDGA EKLVLG
Sbjct: 788 RSGRHSELKIESLNGDGATEKLVLG 714

BLAST of Sgr028190.1 vs. NCBI nr
Match: KAG7024834.1 (putative ubiquitin-conjugating enzyme E2 26 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1010.0 bits (2610), Expect = 1.2e-290
Identity = 509/708 (71.89%), Postives = 574/708 (81.07%), Query Frame = 0

Query: 107 IISLDVIDVDKDEACDNAIFIHDNFDTNDKGKGIYHPSDHQLKETLASRSFSPDVEILES 166
           II LD +DVD+DEA DNA+F HD+FDTNDKGKGIY  SDHQLKET AS S SPDVEI ES
Sbjct: 14  IILLDRVDVDEDEASDNAVFNHDDFDTNDKGKGIYQHSDHQLKETFASHSLSPDVEIQES 73

Query: 167 VNEVKPSFPSASHNLIDLDNHVSDLSNEDDEWVPFDEFMDVDEYSALQAHFDHMDIPPDI 226
            NE + +FPSASHN +++DNH SD SN+ ++W  FDEFMDVD YSALQAHFDHMDIPPDI
Sbjct: 74  PNEDESNFPSASHNFVEIDNHFSDSSNDYEDWTAFDEFMDVDAYSALQAHFDHMDIPPDI 133

Query: 227 EAPIPWLPPHGENRYKSYFEIS---SSFAEPQIEPGSVNQQGKSPAQFKLKATLANSLNM 286
           EAPIPWL PHG+N      E S   SS AEPQ EPG VN  GKSPA  +LK  L+NSLN+
Sbjct: 134 EAPIPWL-PHGQNGNNIDTETSTSWSSLAEPQTEPGPVNHLGKSPAHVQLKEALSNSLNL 193

Query: 287 QAEIDGVNHPPEGIEP---LPSQDVLPTKKSAASRYRDKSVSPHDVSYINSLLSVEAAKS 346
           QA+I   NH PE IEP    PSQDV P +KSA SRYR K    + VS++NS L ++AAKS
Sbjct: 194 QADIGVQNHLPEDIEPFASFPSQDVQPNRKSATSRYRGK----YGVSHLNSALGIDAAKS 253

Query: 347 RWFLDPFKRKKKLYGSNNISINQTDAMKLHNGGETSFDTSDSVKKQVGSHIISHSNFPAY 406
           R FLDPFKRKKKL+  NNISIN +DA+KLHNGGETS DT+DSVKK  G H + HS FP Y
Sbjct: 254 RSFLDPFKRKKKLHELNNISINTSDAIKLHNGGETS-DTADSVKKLAGLHSMPHSKFPGY 313

Query: 407 MDLFNYPPEAGPSIPHWPDYAKWKTKEPFSTPHNAPDFYGPFNPLHPPTLGEVVDMPWIQ 466
           M+ F + PEAG  +PHWPDYAK K KEPF  PH A   YGPF+PL PPTLGEVVDMPW+Q
Sbjct: 314 MEFFKH-PEAGQPMPHWPDYAKMKAKEPFFGPHMASGLYGPFSPLVPPTLGEVVDMPWVQ 373

Query: 467 GSTQNLANLAPGNSTHEIITCKDMDELLRNFERFKQFDTVEDHSDHRYTSSGFTMKQLPR 526
            ST++L N+ P +S +E I   D+DE+L NFE+FKQFDTV+DHSDH YTS GF+MKQ  +
Sbjct: 374 SSTKSLPNIDPDDSINETIAPTDLDEILGNFEQFKQFDTVDDHSDHHYTSKGFSMKQASK 433

Query: 527 KWTKRIQEEWKILEKDLPETIFVRTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSY 586
           KWTK+IQ++WKIL+ DLPETIFVR YESRMDLMRAVIIGA+GTPYHDGLFFFDIFFP +Y
Sbjct: 434 KWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTAY 493

Query: 587 PDVPPQVYYHSGGLRLNPNLYNCGKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLI 646
           PD+PP VYYHS GLRLNPNLYNCGKVCLSLLNTW GSGNE WVPGMSTMLQVLVSIQGLI
Sbjct: 494 PDLPPHVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGSGNENWVPGMSTMLQVLVSIQGLI 553

Query: 647 LNTKPYFNEPGYAYQSGSVAGENKSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFC 706
           LNTKPYFNEPGYAYQSGSVAGEN+SQ YNEEAYILSIKTMV+NIRRPPKHFEDFVRGHF 
Sbjct: 554 LNTKPYFNEPGYAYQSGSVAGENRSQRYNEEAYILSIKTMVFNIRRPPKHFEDFVRGHFF 613

Query: 707 KRAHDILVACKAYMDGAQVGSLVKDGVQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLG 766
           KRAHDILVACKAY+ GAQVGSLVKD V+DL   +KSCS+HFKQ +   L  LVETF ++G
Sbjct: 614 KRAHDILVACKAYIGGAQVGSLVKDRVKDLKPSNKSCSDHFKQCVNHVLSRLVETFKRIG 673

Query: 767 VSDCEKFLPPLPSPSPLVPNARKR-SGRHTKVQLESLEGDGAEKLVLG 808
           VSDCEKFL PL   S  V NA+++ S R +K +LESLEGD  EKLVLG
Sbjct: 674 VSDCEKFLQPL---STSVSNAKEQSSARGSKAELESLEGDDVEKLVLG 711

BLAST of Sgr028190.1 vs. ExPASy Swiss-Prot
Match: Q8GY87 (Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=UBC26 PE=2 SV=2)

HSP 1 Score: 374.4 bits (960), Expect = 3.2e-102
Identity = 181/311 (58.20%), Postives = 228/311 (73.31%), Query Frame = 0

Query: 485 DELLRNFERFKQFDTVEDHSDHRYTSSGFTMKQLPRKWTKRIQEEWKILEKDLPETIFVR 544
           +E LR+   FK+FDTVED SDH Y S G + KQ  + W K++Q +WKILE DLPE I VR
Sbjct: 238 EEFLRD---FKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISVR 297

Query: 545 TYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNLYNCG 604
             ESRMDL+RAVIIGAEGTPYHDGLFFFDI FP +YP VPP V+YHSGGLR+NPNLYNCG
Sbjct: 298 ACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCG 357

Query: 605 KVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVAGENK 664
           KVCLSLL TW+GS  E+W+P  STMLQ+LVSIQ LILN KPYFNEPGY   +G+ +GE+K
Sbjct: 358 KVCLSLLGTWAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESK 417

Query: 665 SQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVGSLVK 724
           S+ Y+E  ++LS+KTMVY+IRRPP+HFE++V+ H+  R+HDI+ AC AY  GA +GS+VK
Sbjct: 418 SKVYSENVFLLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVK 477

Query: 725 DGVQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLGVSDCEKFLPPLPSPSPLVPNARKR 784
            GVQDL+   +S S  FK  +   ++ +V+ F +LGV    K L   P P     N   +
Sbjct: 478 GGVQDLEEARQSGSKKFKTDVASFMQTVVDEFVKLGV----KELAEKPEPPMSNANTENQ 537

Query: 785 SGRHTKVQLES 796
           S + T+ +  S
Sbjct: 538 SKKKTRKRSRS 541

BLAST of Sgr028190.1 vs. ExPASy Swiss-Prot
Match: Q9C918 (Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=UBC38 PE=3 SV=2)

HSP 1 Score: 370.2 bits (949), Expect = 6.1e-101
Identity = 177/311 (56.91%), Postives = 225/311 (72.35%), Query Frame = 0

Query: 485 DELLRNFERFKQFDTVEDHSDHRYTSSGFTMKQLPRKWTKRIQEEWKILEKDLPETIFVR 544
           +E LRN   FK+FD+VED SDH Y S G   KQ  + W K++Q+EWKIL ++LPETIFVR
Sbjct: 21  EEFLRN---FKRFDSVEDFSDHHYVSKGKASKQHSKNWVKKVQDEWKILNQNLPETIFVR 80

Query: 545 TYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNLYNCG 604
             ESRMDL+RAVIIGAEGTPYHDGLFFFDI FP +YP VPP VYYHSGGLR+NPNLYNCG
Sbjct: 81  ACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVYYHSGGLRINPNLYNCG 140

Query: 605 KVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVAGENK 664
           KVCLSLL TW G+  + W+P  STMLQVLVSIQ L+LN +PYFNEPGY    G+  GE+K
Sbjct: 141 KVCLSLLGTWHGNARQSWLPKESTMLQVLVSIQALVLNEQPYFNEPGYGLIKGTWLGESK 200

Query: 665 SQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVGSLVK 724
           S+ Y+E  ++LS+KTMVY++R+PP+HFE++V+ H+  R+HDI+ AC AY  GA +GS+VK
Sbjct: 201 SKVYSENVFLLSLKTMVYSMRKPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVK 260

Query: 725 DGVQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLGVSDCEKFLPPLPSPSPLVPNARKR 784
            GVQDL+   +S S  FK  +   ++ +V+ F +LGV +  +       P P V NA   
Sbjct: 261 GGVQDLEQARQSGSKKFKTDVASFMQTVVDEFVKLGVKELAE------KPKPPVNNANTE 320

Query: 785 SGRHTKVQLES 796
           +    K +  S
Sbjct: 321 NQSKKKTRKRS 322

BLAST of Sgr028190.1 vs. ExPASy Swiss-Prot
Match: Q9LUQ5 (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=UBC25 PE=2 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.7e-98
Identity = 177/286 (61.89%), Postives = 213/286 (74.48%), Query Frame = 0

Query: 482 KDMDELLRNFERFKQFDTVEDHSDHRYTSSGFTMKQLPRKWTKRIQEEWKILEKDLPETI 541
           ++M++ L  +  FK+FD VED SDH Y S G T KQ  + W KRIQ+EW+ILEKDLPE I
Sbjct: 293 RNMEDYLGLYLFFKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDEWRILEKDLPEMI 352

Query: 542 FVRTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNLY 601
           FVR YESRMDL+RAVIIGA+GTPYHDGLFFFDIFFP +YP  PP V+YHSGGLR+NPNLY
Sbjct: 353 FVRAYESRMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLY 412

Query: 602 NCGKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVAG 661
           NCGKVCLSLL TWSG+  E+W+P  STMLQVLVSIQGLILN KPYFNEPGY   +GS  G
Sbjct: 413 NCGKVCLSLLGTWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHG 472

Query: 662 ENKSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVGS 721
           E+ S+ Y+E  +ILS+KTMVY +RRPPK+FEDF  GHF   AHD+L AC AY +GA  G 
Sbjct: 473 ESTSKAYSENTFILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGY 532

Query: 722 LVKDGVQDLDLGDKSCSN-HFKQSLYQHLR-MLVETFTQLGVSDCE 766
           LVK G  D++      S+  F+  +   +  +L++ F  LGV   E
Sbjct: 533 LVK-GAPDVEENSAGMSSLKFRTDVATFVETVLLKEFILLGVLGLE 577

BLAST of Sgr028190.1 vs. ExPASy Swiss-Prot
Match: F4HPP7 (Putative ubiquitin-conjugating enzyme E2 39 OS=Arabidopsis thaliana OX=3702 GN=UBC39 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.8e-85
Identity = 161/294 (54.76%), Postives = 203/294 (69.05%), Query Frame = 0

Query: 477 EIITCKDMDELLRNFER-FKQFDTVEDHSDHRYTSSG---------FTMKQLPRKWTKRI 536
           EI    ++ +L   F R FK+FDTVED SDH Y + G            K  P+ W K I
Sbjct: 3   EIKDSSNVRKLKEEFLRDFKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDI 62

Query: 537 QEEWKILEKDLPETIFVRTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQ 596
           Q+EWKIL+K+LPETIFVR  ESR+DL+RAVIIGAEGTPYHDGLFFFDI FP +YP VPP+
Sbjct: 63  QKEWKILDKNLPETIFVRACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPK 122

Query: 597 VYYHSGGLRLNPNLYNCGKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPY 656
           V+YHSGGLR+NPNLY CGKVCLSL++TW+G   E+W+P  STMLQ+LVSIQ LILN KPY
Sbjct: 123 VHYHSGGLRINPNLYKCGKVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPY 182

Query: 657 FNEPGYAYQSGSVAGENKSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDI 716
           +NEPGY    G+  GE+ S+ Y+E  ++ S+KTM         HFE+FVR HF  R+HDI
Sbjct: 183 YNEPGYEKSMGTPLGESYSKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDI 242

Query: 717 LVACKAYMDGAQVGSLVKDGVQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLG 761
           + AC AY DGA VGS+ K GV+      +  S  F+ ++   ++ +V+ F  LG
Sbjct: 243 VKACNAYKDGAPVGSIDKGGVKKQT--RQRGSLKFRINVTSFMKTVVDEFVNLG 285

BLAST of Sgr028190.1 vs. ExPASy Swiss-Prot
Match: Q8VY10 (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=UBC24 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 7.5e-83
Identity = 151/284 (53.17%), Postives = 191/284 (67.25%), Query Frame = 0

Query: 489 RNFERFKQFDTVEDHSDHRYTSS--GFTMKQLPRKWTKRIQEEWKILEKDLPETIFVRTY 548
           RN  RF+QFD V D SDH + SS  G    Q+ + W K++Q+EW  LE +LP TI+VR  
Sbjct: 628 RNQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVC 687

Query: 549 ESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNLYNCGKV 608
           E RMDL+RA ++GA GTPYHDGLFFFDI  PP YP  PP V+YHSGG+RLNPNLY  G+V
Sbjct: 688 EERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRV 747

Query: 609 CLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVAGENKSQ 668
           CLSLLNTWSGSG E W  G S++LQ+L+S Q L+LN KPYFNE GY  Q G   GE  S 
Sbjct: 748 CLSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSV 807

Query: 669 HYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVGSLVKDG 728
            YNE A++++ K+M+  +R+PPKHFE  V+ HF  RA  +L ACKAYM+G  VGS     
Sbjct: 808 SYNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGSSA--- 867

Query: 729 VQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLGVSDCEKFLPP 771
             +L     + S  FK  L +    L+E F+++GV DC + + P
Sbjct: 868 --NLQGNSTTNSTGFKIMLSKLYPKLLEAFSEIGV-DCVQEIGP 905

BLAST of Sgr028190.1 vs. ExPASy TrEMBL
Match: A0A6J1CFX7 (probable ubiquitin-conjugating enzyme E2 26 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010440 PE=4 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 548/712 (76.97%), Postives = 608/712 (85.39%), Query Frame = 0

Query: 107 IISLDVIDVDKDEACDNAIFIHDNFDTNDKGKGIYHPSDHQLKETLASRSFSPDVEILES 166
           I S++VIDVDKDE  +N I+IHD  + NDKGKGIYH SDH+L+E +AS S+SPDVEILE 
Sbjct: 24  IQSVEVIDVDKDEDFNNVIYIHDGVENNDKGKGIYHNSDHELQEMMASPSYSPDVEILEG 83

Query: 167 VNEVKPSFPSASHNLIDLDNHVSDLSNEDDEWVPFDEFMDVDEYSALQAHFDHMDIPPDI 226
            NEV+ +FPSA HN+IDLDNH SD SN  DEW+PFDE MDVDEYSALQAHFDHMDIPPDI
Sbjct: 84  GNEVESAFPSAQHNVIDLDNHYSDSSN-GDEWIPFDEIMDVDEYSALQAHFDHMDIPPDI 143

Query: 227 EAPIPWLPPHGENRYKSYFEISSSFAEPQIEPGSVNQQGKSPAQFKLKATLANSLNMQAE 286
           EAPIPWL PHG+NR+KS F  SSSFAE QI PGSVNQ G SPA  KLKATLANSLN+QAE
Sbjct: 144 EAPIPWL-PHGQNRHKSIFGTSSSFAESQINPGSVNQLGNSPAHAKLKATLANSLNLQAE 203

Query: 287 IDGVNHPPEGIEP---LPSQDVLPTKKSAASRYRDKSVSPHDVSYINSLLSVEAAKSRWF 346
           ID VNHPPEG EP     SQDVLP KKS ASRYRDKS  PH+V  INSLL  EAAK+RWF
Sbjct: 204 IDIVNHPPEGTEPFTSFTSQDVLPNKKSVASRYRDKSGLPHEV--INSLLGAEAAKTRWF 263

Query: 347 LDPFKRKKKLYGSNNISINQTDAMKLH-NGGETSFDTSDSVKKQVGSHIISHSNFPAYMD 406
            DPFKRKKKL+GSNNISINQ+D+ KLH NGGETS DTS++VKKQ  S+ +S  NFPAYMD
Sbjct: 264 SDPFKRKKKLHGSNNISINQSDSRKLHNNGGETSSDTSETVKKQFSSNNMSSFNFPAYMD 323

Query: 407 LFNYPPEAGPSIPHWPDYAKWKTKEPFSTPHNAPDFYGPF------NPLHPPTLGEVVDM 466
              YPP AG S+PHWPDYAK KTKE    P +  D+YGPF      +PLHPPTLGEVVDM
Sbjct: 324 NIYYPPGAGASLPHWPDYAKMKTKELVLNPPSVSDYYGPFDHLIPPHPLHPPTLGEVVDM 383

Query: 467 PWIQGSTQNLANLAPGNSTHEIITCKDMDELLRNFERFKQFDTVEDHSDHRYTSSGFTMK 526
           PWIQGST++ ANLAP NST E I+  D+DE++R FE+FK+FDTV+DHSDH YTS GFTMK
Sbjct: 384 PWIQGSTRDPANLAPDNSTLETISIADLDEIMRKFEQFKRFDTVDDHSDHHYTSKGFTMK 443

Query: 527 QLPRKWTKRIQEEWKILEKDLPETIFVRTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFF 586
           QLP+KWTKRIQEEWKILEKDLP+TIFVR YESRMDL+RAVIIGAEGTPYHDGLFFFDIFF
Sbjct: 444 QLPKKWTKRIQEEWKILEKDLPDTIFVRAYESRMDLLRAVIIGAEGTPYHDGLFFFDIFF 503

Query: 587 PPSYPDVPPQVYYHSGGLRLNPNLYNCGKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSI 646
           P  YPDVPP VYYHSGGLRLNPNLYNCGKVCLSLLNTWSGSGNERW+PGMSTMLQVLVSI
Sbjct: 504 PMGYPDVPPLVYYHSGGLRLNPNLYNCGKVCLSLLNTWSGSGNERWIPGMSTMLQVLVSI 563

Query: 647 QGLILNTKPYFNEPGYAYQSGSVAGENKSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVR 706
           QGLILNTKPYFNEPG+A Q+GSVAGEN+SQ YNEE Y+LS+KTMVYNIRRPPKHFEDFV+
Sbjct: 564 QGLILNTKPYFNEPGFANQNGSVAGENRSQQYNEETYLLSMKTMVYNIRRPPKHFEDFVK 623

Query: 707 GHFCKRAHDILVACKAYMDGAQVGSLVKDGVQDLDLGDKSCSNHFKQSLYQHLRMLVETF 766
           GHF KRAHDILV+CKAYMDGAQVGSLVK GVQDLDL DK CSNHFKQSL Q+L MLV+TF
Sbjct: 624 GHFFKRAHDILVSCKAYMDGAQVGSLVKGGVQDLDLSDKCCSNHFKQSLSQYLSMLVKTF 683

Query: 767 TQLGVSDCEKFLPPLPSPSPLVPNARKRSGRHTKVQLESLEGDGA-EKLVLG 808
            ++GV DCEKFL  LP PS  V +A+KRSGRH+++++ESL GDGA EKLVLG
Sbjct: 684 QRMGVGDCEKFL--LPPPSLPVSSAKKRSGRHSELKIESLNGDGATEKLVLG 729

BLAST of Sgr028190.1 vs. ExPASy TrEMBL
Match: A0A6J1CEB1 (probable ubiquitin-conjugating enzyme E2 26 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010440 PE=4 SV=1)

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 568/808 (70.30%), Postives = 635/808 (78.59%), Query Frame = 0

Query: 13  DHQMEPP--PNESQNSKSSISFFFFPILLSFLCFFLNFLFLFLSLLLCFSNLSDCFSAMT 72
           DHQMEPP  PN SQNSK+ +                                    S   
Sbjct: 8   DHQMEPPPSPNSSQNSKTKL-----------------------------------LSGTG 67

Query: 73  SGKEKNLASVLDTLNTLKIDLNMDRVTESDISSAMSIISLDVIDVDKDEACDNAIFIHDN 132
           SG  K           L+    ++R  +  +     I S++VIDVDKDE  +N I+IHD 
Sbjct: 68  SGSMKP--------QVLRHSPLINRPPK--MLKNKGIQSVEVIDVDKDEDFNNVIYIHDG 127

Query: 133 FDTNDKGKGIYHPSDHQLKETLASRSFSPDVEILESVNEVKPSFPSASHNLIDLDNHVSD 192
            + NDKGKGIYH SDH+L+E +AS S+SPDVEILE  NEV+ +FPSA HN+IDLDNH SD
Sbjct: 128 VENNDKGKGIYHNSDHELQEMMASPSYSPDVEILEGGNEVESAFPSAQHNVIDLDNHYSD 187

Query: 193 LSNEDDEWVPFDEFMDVDEYSALQAHFDHMDIPPDIEAPIPWLPPHGENRYKSYFEISSS 252
            SN  DEW+PFDE MDVDEYSALQAHFDHMDIPPDIEAPIPWL PHG+NR+KS F  SSS
Sbjct: 188 SSN-GDEWIPFDEIMDVDEYSALQAHFDHMDIPPDIEAPIPWL-PHGQNRHKSIFGTSSS 247

Query: 253 FAEPQIEPGSVNQQGKSPAQFKLKATLANSLNMQAEIDGVNHPPEGIEP---LPSQDVLP 312
           FAE QI PGSVNQ G SPA  KLKATLANSLN+QAEID VNHPPEG EP     SQDVLP
Sbjct: 248 FAESQINPGSVNQLGNSPAHAKLKATLANSLNLQAEIDIVNHPPEGTEPFTSFTSQDVLP 307

Query: 313 TKKSAASRYRDKSVSPHDVSYINSLLSVEAAKSRWFLDPFKRKKKLYGSNNISINQTDAM 372
            KKS ASRYRDKS  PH+V  INSLL  EAAK+RWF DPFKRKKKL+GSNNISINQ+D+ 
Sbjct: 308 NKKSVASRYRDKSGLPHEV--INSLLGAEAAKTRWFSDPFKRKKKLHGSNNISINQSDSR 367

Query: 373 KLH-NGGETSFDTSDSVKKQVGSHIISHSNFPAYMDLFNYPPEAGPSIPHWPDYAKWKTK 432
           KLH NGGETS DTS++VKKQ  S+ +S  NFPAYMD   YPP AG S+PHWPDYAK KTK
Sbjct: 368 KLHNNGGETSSDTSETVKKQFSSNNMSSFNFPAYMDNIYYPPGAGASLPHWPDYAKMKTK 427

Query: 433 EPFSTPHNAPDFYGPF------NPLHPPTLGEVVDMPWIQGSTQNLANLAPGNSTHEIIT 492
           E    P +  D+YGPF      +PLHPPTLGEVVDMPWIQGST++ ANLAP NST E I+
Sbjct: 428 ELVLNPPSVSDYYGPFDHLIPPHPLHPPTLGEVVDMPWIQGSTRDPANLAPDNSTLETIS 487

Query: 493 CKDMDELLRNFERFKQFDTVEDHSDHRYTSSGFTMKQLPRKWTKRIQEEWKILEKDLPET 552
             D+DE++R FE+FK+FDTV+DHSDH YTS GFTMKQLP+KWTKRIQEEWKILEKDLP+T
Sbjct: 488 IADLDEIMRKFEQFKRFDTVDDHSDHHYTSKGFTMKQLPKKWTKRIQEEWKILEKDLPDT 547

Query: 553 IFVRTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNL 612
           IFVR YESRMDL+RAVIIGAEGTPYHDGLFFFDIFFP  YPDVPP VYYHSGGLRLNPNL
Sbjct: 548 IFVRAYESRMDLLRAVIIGAEGTPYHDGLFFFDIFFPMGYPDVPPLVYYHSGGLRLNPNL 607

Query: 613 YNCGKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVA 672
           YNCGKVCLSLLNTWSGSGNERW+PGMSTMLQVLVSIQGLILNTKPYFNEPG+A Q+GSVA
Sbjct: 608 YNCGKVCLSLLNTWSGSGNERWIPGMSTMLQVLVSIQGLILNTKPYFNEPGFANQNGSVA 667

Query: 673 GENKSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVG 732
           GEN+SQ YNEE Y+LS+KTMVYNIRRPPKHFEDFV+GHF KRAHDILV+CKAYMDGAQVG
Sbjct: 668 GENRSQQYNEETYLLSMKTMVYNIRRPPKHFEDFVKGHFFKRAHDILVSCKAYMDGAQVG 727

Query: 733 SLVKDGVQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLGVSDCEKFLPPLPSPSPLVPN 792
           SLVK GVQDLDL DK CSNHFKQSL Q+L MLV+TF ++GV DCEKFL  LP PS  V +
Sbjct: 728 SLVKGGVQDLDLSDKCCSNHFKQSLSQYLSMLVKTFQRMGVGDCEKFL--LPPPSLPVSS 764

Query: 793 ARKRSGRHTKVQLESLEGDGA-EKLVLG 808
           A+KRSGRH+++++ESL GDGA EKLVLG
Sbjct: 788 AKKRSGRHSELKIESLNGDGATEKLVLG 764

BLAST of Sgr028190.1 vs. ExPASy TrEMBL
Match: A0A6J1CDF3 (uncharacterized protein LOC111010440 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111010440 PE=4 SV=1)

HSP 1 Score: 1029.6 bits (2661), Expect = 6.9e-297
Identity = 538/805 (66.83%), Postives = 604/805 (75.03%), Query Frame = 0

Query: 13  DHQMEPP--PNESQNSKSSISFFFFPILLSFLCFFLNFLFLFLSLLLCFSNLSDCFSAMT 72
           DHQMEPP  PN SQNSK+ +                                    S   
Sbjct: 8   DHQMEPPPSPNSSQNSKTKL-----------------------------------LSGTG 67

Query: 73  SGKEKNLASVLDTLNTLKIDLNMDRVTESDISSAMSIISLDVIDVDKDEACDNAIFIHDN 132
           SG  K           L+    ++R  +  +     I S++VIDVDKDE  +N I+IHD 
Sbjct: 68  SGSMKP--------QVLRHSPLINRPPK--MLKNKGIQSVEVIDVDKDEDFNNVIYIHDG 127

Query: 133 FDTNDKGKGIYHPSDHQLKETLASRSFSPDVEILESVNEVKPSFPSASHNLIDLDNHVSD 192
            + NDKGKGIYH SDH+L+E +AS S+SPDVEILE  NEV+ +FPSA HN+IDLDNH SD
Sbjct: 128 VENNDKGKGIYHNSDHELQEMMASPSYSPDVEILEGGNEVESAFPSAQHNVIDLDNHYSD 187

Query: 193 LSNEDDEWVPFDEFMDVDEYSALQAHFDHMDIPPDIEAPIPWLPPHGENRYKSYFEISSS 252
            SN  DEW+PFDE MDVDEYSALQAHFDHMDIPPDIEAPIPWL PHG+NR+KS F  SSS
Sbjct: 188 SSN-GDEWIPFDEIMDVDEYSALQAHFDHMDIPPDIEAPIPWL-PHGQNRHKSIFGTSSS 247

Query: 253 FAEPQIEPGSVNQQGKSPAQFKLKATLANSLNMQAEIDGVNHPPEGIEPLPSQDVLPTKK 312
           FAE QI PGSVNQ G SPA  KLKATLANSLN+QAEID VNHPPE               
Sbjct: 248 FAESQINPGSVNQLGNSPAHAKLKATLANSLNLQAEIDIVNHPPE--------------- 307

Query: 313 SAASRYRDKSVSPHDVSYINSLLSVEAAKSRWFLDPFKRKKKLYGSNNISINQTDAMKLH 372
                                      AK+RWF DPFKRKKKL+GSNNISINQ+D+ KLH
Sbjct: 308 ---------------------------AKTRWFSDPFKRKKKLHGSNNISINQSDSRKLH 367

Query: 373 -NGGETSFDTSDSVKKQVGSHIISHSNFPAYMDLFNYPPEAGPSIPHWPDYAKWKTKEPF 432
            NGGETS DTS++VKKQ  S+ +S  NFPAYMD   YPP AG S+PHWPDYAK KTKE  
Sbjct: 368 NNGGETSSDTSETVKKQFSSNNMSSFNFPAYMDNIYYPPGAGASLPHWPDYAKMKTKELV 427

Query: 433 STPHNAPDFYGPF------NPLHPPTLGEVVDMPWIQGSTQNLANLAPGNSTHEIITCKD 492
             P +  D+YGPF      +PLHPPTLGEVVDMPWIQGST++ ANLAP NST E I+  D
Sbjct: 428 LNPPSVSDYYGPFDHLIPPHPLHPPTLGEVVDMPWIQGSTRDPANLAPDNSTLETISIAD 487

Query: 493 MDELLRNFERFKQFDTVEDHSDHRYTSSGFTMKQLPRKWTKRIQEEWKILEKDLPETIFV 552
           +DE++R FE+FK+FDTV+DHSDH YTS GFTMKQLP+KWTKRIQEEWKILEKDLP+TIFV
Sbjct: 488 LDEIMRKFEQFKRFDTVDDHSDHHYTSKGFTMKQLPKKWTKRIQEEWKILEKDLPDTIFV 547

Query: 553 RTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNLYNC 612
           R YESRMDL+RAVIIGAEGTPYHDGLFFFDIFFP  YPDVPP VYYHSGGLRLNPNLYNC
Sbjct: 548 RAYESRMDLLRAVIIGAEGTPYHDGLFFFDIFFPMGYPDVPPLVYYHSGGLRLNPNLYNC 607

Query: 613 GKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVAGEN 672
           GKVCLSLLNTWSGSGNERW+PGMSTMLQVLVSIQGLILNTKPYFNEPG+A Q+GSVAGEN
Sbjct: 608 GKVCLSLLNTWSGSGNERWIPGMSTMLQVLVSIQGLILNTKPYFNEPGFANQNGSVAGEN 667

Query: 673 KSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVGSLV 732
           +SQ YNEE Y+LS+KTMVYNIRRPPKHFEDFV+GHF KRAHDILV+CKAYMDGAQVGSLV
Sbjct: 668 RSQQYNEETYLLSMKTMVYNIRRPPKHFEDFVKGHFFKRAHDILVSCKAYMDGAQVGSLV 721

Query: 733 KDGVQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLGVSDCEKFLPPLPSPSPLVPNARK 792
           K GVQDLDL DK CSNHFKQSL Q+L MLV+TF ++GV DCEKFL  LP PS  V +A+K
Sbjct: 728 KGGVQDLDLSDKCCSNHFKQSLSQYLSMLVKTFQRMGVGDCEKFL--LPPPSLPVSSAKK 721

Query: 793 RSGRHTKVQLESLEGDGA-EKLVLG 808
           RSGRH+++++ESL GDGA EKLVLG
Sbjct: 788 RSGRHSELKIESLNGDGATEKLVLG 721

BLAST of Sgr028190.1 vs. ExPASy TrEMBL
Match: A0A6J1CCN6 (probable ubiquitin-conjugating enzyme E2 25 isoform X4 OS=Momordica charantia OX=3673 GN=LOC111010440 PE=4 SV=1)

HSP 1 Score: 1015.0 bits (2623), Expect = 1.7e-292
Identity = 533/805 (66.21%), Postives = 598/805 (74.29%), Query Frame = 0

Query: 13  DHQMEPP--PNESQNSKSSISFFFFPILLSFLCFFLNFLFLFLSLLLCFSNLSDCFSAMT 72
           DHQMEPP  PN SQNSK+ +                                    S   
Sbjct: 8   DHQMEPPPSPNSSQNSKTKL-----------------------------------LSGTG 67

Query: 73  SGKEKNLASVLDTLNTLKIDLNMDRVTESDISSAMSIISLDVIDVDKDEACDNAIFIHDN 132
           SG  K           L+    ++R  +  +     I S++VIDVDKDE  +N I+IHD 
Sbjct: 68  SGSMKP--------QVLRHSPLINRPPK--MLKNKGIQSVEVIDVDKDEDFNNVIYIHDG 127

Query: 133 FDTNDKGKGIYHPSDHQLKETLASRSFSPDVEILESVNEVKPSFPSASHNLIDLDNHVSD 192
            + NDKGKGIYH SDH+L+E +AS S+SPDVEILE  NEV+ +FPSA HN+IDLDNH SD
Sbjct: 128 VENNDKGKGIYHNSDHELQEMMASPSYSPDVEILEGGNEVESAFPSAQHNVIDLDNHYSD 187

Query: 193 LSNEDDEWVPFDEFMDVDEYSALQAHFDHMDIPPDIEAPIPWLPPHGENRYKSYFEISSS 252
            SN  DEW+PFDE MDVDEYSALQAHFDHMDIPPDIEAPIPWL PHG+NR+KS F  SSS
Sbjct: 188 SSN-GDEWIPFDEIMDVDEYSALQAHFDHMDIPPDIEAPIPWL-PHGQNRHKSIFGTSSS 247

Query: 253 FAEPQIEPGSVNQQGKSPAQFKLKATLANSLNMQAEIDGVNHPPEGIEPLPSQDVLPTKK 312
           FAE QI PGSVNQ G SPA  KLKATLANSLN+QAEID VNHPPE               
Sbjct: 248 FAESQINPGSVNQLGNSPAHAKLKATLANSLNLQAEIDIVNHPPE--------------- 307

Query: 313 SAASRYRDKSVSPHDVSYINSLLSVEAAKSRWFLDPFKRKKKLYGSNNISINQTDAMKLH 372
                                             DPFKRKKKL+GSNNISINQ+D+ KLH
Sbjct: 308 ----------------------------------DPFKRKKKLHGSNNISINQSDSRKLH 367

Query: 373 -NGGETSFDTSDSVKKQVGSHIISHSNFPAYMDLFNYPPEAGPSIPHWPDYAKWKTKEPF 432
            NGGETS DTS++VKKQ  S+ +S  NFPAYMD   YPP AG S+PHWPDYAK KTKE  
Sbjct: 368 NNGGETSSDTSETVKKQFSSNNMSSFNFPAYMDNIYYPPGAGASLPHWPDYAKMKTKELV 427

Query: 433 STPHNAPDFYGPF------NPLHPPTLGEVVDMPWIQGSTQNLANLAPGNSTHEIITCKD 492
             P +  D+YGPF      +PLHPPTLGEVVDMPWIQGST++ ANLAP NST E I+  D
Sbjct: 428 LNPPSVSDYYGPFDHLIPPHPLHPPTLGEVVDMPWIQGSTRDPANLAPDNSTLETISIAD 487

Query: 493 MDELLRNFERFKQFDTVEDHSDHRYTSSGFTMKQLPRKWTKRIQEEWKILEKDLPETIFV 552
           +DE++R FE+FK+FDTV+DHSDH YTS GFTMKQLP+KWTKRIQEEWKILEKDLP+TIFV
Sbjct: 488 LDEIMRKFEQFKRFDTVDDHSDHHYTSKGFTMKQLPKKWTKRIQEEWKILEKDLPDTIFV 547

Query: 553 RTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNLYNC 612
           R YESRMDL+RAVIIGAEGTPYHDGLFFFDIFFP  YPDVPP VYYHSGGLRLNPNLYNC
Sbjct: 548 RAYESRMDLLRAVIIGAEGTPYHDGLFFFDIFFPMGYPDVPPLVYYHSGGLRLNPNLYNC 607

Query: 613 GKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVAGEN 672
           GKVCLSLLNTWSGSGNERW+PGMSTMLQVLVSIQGLILNTKPYFNEPG+A Q+GSVAGEN
Sbjct: 608 GKVCLSLLNTWSGSGNERWIPGMSTMLQVLVSIQGLILNTKPYFNEPGFANQNGSVAGEN 667

Query: 673 KSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVGSLV 732
           +SQ YNEE Y+LS+KTMVYNIRRPPKHFEDFV+GHF KRAHDILV+CKAYMDGAQVGSLV
Sbjct: 668 RSQQYNEETYLLSMKTMVYNIRRPPKHFEDFVKGHFFKRAHDILVSCKAYMDGAQVGSLV 714

Query: 733 KDGVQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLGVSDCEKFLPPLPSPSPLVPNARK 792
           K GVQDLDL DK CSNHFKQSL Q+L MLV+TF ++GV DCEKFL  LP PS  V +A+K
Sbjct: 728 KGGVQDLDLSDKCCSNHFKQSLSQYLSMLVKTFQRMGVGDCEKFL--LPPPSLPVSSAKK 714

Query: 793 RSGRHTKVQLESLEGDGA-EKLVLG 808
           RSGRH+++++ESL GDGA EKLVLG
Sbjct: 788 RSGRHSELKIESLNGDGATEKLVLG 714

BLAST of Sgr028190.1 vs. ExPASy TrEMBL
Match: A0A6J1FEJ0 (uncharacterized protein LOC111443352 OS=Cucurbita moschata OX=3662 GN=LOC111443352 PE=4 SV=1)

HSP 1 Score: 1006.1 bits (2600), Expect = 8.1e-290
Identity = 506/705 (71.77%), Postives = 572/705 (81.13%), Query Frame = 0

Query: 110 LDVIDVDKDEACDNAIFIHDNFDTNDKGKGIYHPSDHQLKETLASRSFSPDVEILESVNE 169
           LD +DVD+DEA +NA+F HD+FDTNDKGKGIY  SDHQLKET AS S SPDVEI ES NE
Sbjct: 53  LDRVDVDEDEASNNAVFNHDDFDTNDKGKGIYQHSDHQLKETFASHSLSPDVEIQESPNE 112

Query: 170 VKPSFPSASHNLIDLDNHVSDLSNEDDEWVPFDEFMDVDEYSALQAHFDHMDIPPDIEAP 229
            + +FPSASHN +++DNH SD SN+ ++W  FDEFMDVD YSALQAHFDHMDIPPDIEAP
Sbjct: 113 DESNFPSASHNFVEIDNHFSDSSNDYEDWTAFDEFMDVDAYSALQAHFDHMDIPPDIEAP 172

Query: 230 IPWLPPHGENRYKSYFEIS---SSFAEPQIEPGSVNQQGKSPAQFKLKATLANSLNMQAE 289
           IPWL PHG+N      E S   SS AEPQ EPG VN  GKSPA  +LK  L+NSLN+QA+
Sbjct: 173 IPWL-PHGQNGNNIDTETSTSWSSLAEPQTEPGPVNHLGKSPAHVQLKEALSNSLNLQAD 232

Query: 290 IDGVNHPPEGIEP---LPSQDVLPTKKSAASRYRDKSVSPHDVSYINSLLSVEAAKSRWF 349
           I   NH PE IEP    PSQDV P +KSA SRYR K    + VS++NS L ++AAKSR F
Sbjct: 233 IGVQNHLPEDIEPFASFPSQDVQPNRKSATSRYRGK----YGVSHLNSALGIDAAKSRSF 292

Query: 350 LDPFKRKKKLYGSNNISINQTDAMKLHNGGETSFDTSDSVKKQVGSHIISHSNFPAYMDL 409
           LDPFKRKKKL+  NNISIN +DA+KLHNGGETS DT+DSVKK  G H + HS FP YM+ 
Sbjct: 293 LDPFKRKKKLHELNNISINTSDAIKLHNGGETS-DTADSVKKLAGLHSMPHSKFPGYMEF 352

Query: 410 FNYPPEAGPSIPHWPDYAKWKTKEPFSTPHNAPDFYGPFNPLHPPTLGEVVDMPWIQGST 469
           F + PEAG  +PHWPDYAK K KEPF  PH A   YGPF+PL PPTLGEVVDMPW+Q ST
Sbjct: 353 FKH-PEAGQPMPHWPDYAKMKAKEPFFGPHMASGLYGPFSPLVPPTLGEVVDMPWVQSST 412

Query: 470 QNLANLAPGNSTHEIITCKDMDELLRNFERFKQFDTVEDHSDHRYTSSGFTMKQLPRKWT 529
           ++L N+ P +S +E I   D+DE+L NFE+FKQFDTV+DHSDH YTS GF+MKQ  +KWT
Sbjct: 413 KSLPNIDPDDSINETIAPTDLDEILGNFEQFKQFDTVDDHSDHHYTSKGFSMKQASKKWT 472

Query: 530 KRIQEEWKILEKDLPETIFVRTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDV 589
           K+IQ++WKIL+ DLPETIFVR YESRMDLMRAVIIGA+GTPYHDGLFFFDIFFP +YPD+
Sbjct: 473 KKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTAYPDL 532

Query: 590 PPQVYYHSGGLRLNPNLYNCGKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNT 649
           PP VYYHS GLRLNPNLYNCGKVCLSLLNTW GSGNE WVPGMSTMLQVLVSIQGLILNT
Sbjct: 533 PPHVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGSGNENWVPGMSTMLQVLVSIQGLILNT 592

Query: 650 KPYFNEPGYAYQSGSVAGENKSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRA 709
           KPYFNEPGYAYQSGSVAGEN+SQ YNEEAYILSIKTMV+NIRRPPKHFEDFVRGHF KRA
Sbjct: 593 KPYFNEPGYAYQSGSVAGENRSQRYNEEAYILSIKTMVFNIRRPPKHFEDFVRGHFFKRA 652

Query: 710 HDILVACKAYMDGAQVGSLVKDGVQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLGVSD 769
           HDILVACKAY+ GAQVGSLVKD V+DL   +KSCS+HFKQ +   L  LVETF ++GVSD
Sbjct: 653 HDILVACKAYIGGAQVGSLVKDRVKDLKPSNKSCSDHFKQCVNHVLSRLVETFKRIGVSD 712

Query: 770 CEKFLPPLPSPSPLVPNARKR-SGRHTKVQLESLEGDGAEKLVLG 808
           CEKFL PL   S  V NA+++ S R +K +LESLEGD  EKLVLG
Sbjct: 713 CEKFLQPL---STSVSNAKEQSSARGSKAELESLEGDDVEKLVLG 747

BLAST of Sgr028190.1 vs. TAIR 10
Match: AT1G53025.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 374.4 bits (960), Expect = 2.3e-103
Identity = 181/311 (58.20%), Postives = 228/311 (73.31%), Query Frame = 0

Query: 485 DELLRNFERFKQFDTVEDHSDHRYTSSGFTMKQLPRKWTKRIQEEWKILEKDLPETIFVR 544
           +E LR+   FK+FDTVED SDH Y S G + KQ  + W K++Q +WKILE DLPE I VR
Sbjct: 238 EEFLRD---FKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISVR 297

Query: 545 TYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNLYNCG 604
             ESRMDL+RAVIIGAEGTPYHDGLFFFDI FP +YP VPP V+YHSGGLR+NPNLYNCG
Sbjct: 298 ACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCG 357

Query: 605 KVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVAGENK 664
           KVCLSLL TW+GS  E+W+P  STMLQ+LVSIQ LILN KPYFNEPGY   +G+ +GE+K
Sbjct: 358 KVCLSLLGTWAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESK 417

Query: 665 SQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVGSLVK 724
           S+ Y+E  ++LS+KTMVY+IRRPP+HFE++V+ H+  R+HDI+ AC AY  GA +GS+VK
Sbjct: 418 SKVYSENVFLLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVK 477

Query: 725 DGVQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLGVSDCEKFLPPLPSPSPLVPNARKR 784
            GVQDL+   +S S  FK  +   ++ +V+ F +LGV    K L   P P     N   +
Sbjct: 478 GGVQDLEEARQSGSKKFKTDVASFMQTVVDEFVKLGV----KELAEKPEPPMSNANTENQ 537

Query: 785 SGRHTKVQLES 796
           S + T+ +  S
Sbjct: 538 SKKKTRKRSRS 541

BLAST of Sgr028190.1 vs. TAIR 10
Match: AT3G15355.1 (ubiquitin-conjugating enzyme 25 )

HSP 1 Score: 362.1 bits (928), Expect = 1.2e-99
Identity = 177/286 (61.89%), Postives = 213/286 (74.48%), Query Frame = 0

Query: 482 KDMDELLRNFERFKQFDTVEDHSDHRYTSSGFTMKQLPRKWTKRIQEEWKILEKDLPETI 541
           ++M++ L  +  FK+FD VED SDH Y S G T KQ  + W KRIQ+EW+ILEKDLPE I
Sbjct: 293 RNMEDYLGLYLFFKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDEWRILEKDLPEMI 352

Query: 542 FVRTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNLY 601
           FVR YESRMDL+RAVIIGA+GTPYHDGLFFFDIFFP +YP  PP V+YHSGGLR+NPNLY
Sbjct: 353 FVRAYESRMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLY 412

Query: 602 NCGKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVAG 661
           NCGKVCLSLL TWSG+  E+W+P  STMLQVLVSIQGLILN KPYFNEPGY   +GS  G
Sbjct: 413 NCGKVCLSLLGTWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHG 472

Query: 662 ENKSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVGS 721
           E+ S+ Y+E  +ILS+KTMVY +RRPPK+FEDF  GHF   AHD+L AC AY +GA  G 
Sbjct: 473 ESTSKAYSENTFILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGY 532

Query: 722 LVKDGVQDLDLGDKSCSN-HFKQSLYQHLR-MLVETFTQLGVSDCE 766
           LVK G  D++      S+  F+  +   +  +L++ F  LGV   E
Sbjct: 533 LVK-GAPDVEENSAGMSSLKFRTDVATFVETVLLKEFILLGVLGLE 577

BLAST of Sgr028190.1 vs. TAIR 10
Match: AT1G53023.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 318.2 bits (814), Expect = 2.0e-86
Identity = 161/294 (54.76%), Postives = 203/294 (69.05%), Query Frame = 0

Query: 477 EIITCKDMDELLRNFER-FKQFDTVEDHSDHRYTSSG---------FTMKQLPRKWTKRI 536
           EI    ++ +L   F R FK+FDTVED SDH Y + G            K  P+ W K I
Sbjct: 3   EIKDSSNVRKLKEEFLRDFKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDI 62

Query: 537 QEEWKILEKDLPETIFVRTYESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQ 596
           Q+EWKIL+K+LPETIFVR  ESR+DL+RAVIIGAEGTPYHDGLFFFDI FP +YP VPP+
Sbjct: 63  QKEWKILDKNLPETIFVRACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPK 122

Query: 597 VYYHSGGLRLNPNLYNCGKVCLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPY 656
           V+YHSGGLR+NPNLY CGKVCLSL++TW+G   E+W+P  STMLQ+LVSIQ LILN KPY
Sbjct: 123 VHYHSGGLRINPNLYKCGKVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPY 182

Query: 657 FNEPGYAYQSGSVAGENKSQHYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDI 716
           +NEPGY    G+  GE+ S+ Y+E  ++ S+KTM         HFE+FVR HF  R+HDI
Sbjct: 183 YNEPGYEKSMGTPLGESYSKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDI 242

Query: 717 LVACKAYMDGAQVGSLVKDGVQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLG 761
           + AC AY DGA VGS+ K GV+      +  S  F+ ++   ++ +V+ F  LG
Sbjct: 243 VKACNAYKDGAPVGSIDKGGVKKQT--RQRGSLKFRINVTSFMKTVVDEFVNLG 285

BLAST of Sgr028190.1 vs. TAIR 10
Match: AT2G33770.1 (phosphate 2 )

HSP 1 Score: 310.1 bits (793), Expect = 5.3e-84
Identity = 151/284 (53.17%), Postives = 191/284 (67.25%), Query Frame = 0

Query: 489 RNFERFKQFDTVEDHSDHRYTSS--GFTMKQLPRKWTKRIQEEWKILEKDLPETIFVRTY 548
           RN  RF+QFD V D SDH + SS  G    Q+ + W K++Q+EW  LE +LP TI+VR  
Sbjct: 628 RNQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVC 687

Query: 549 ESRMDLMRAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNLYNCGKV 608
           E RMDL+RA ++GA GTPYHDGLFFFDI  PP YP  PP V+YHSGG+RLNPNLY  G+V
Sbjct: 688 EERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRV 747

Query: 609 CLSLLNTWSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVAGENKSQ 668
           CLSLLNTWSGSG E W  G S++LQ+L+S Q L+LN KPYFNE GY  Q G   GE  S 
Sbjct: 748 CLSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSV 807

Query: 669 HYNEEAYILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVGSLVKDG 728
            YNE A++++ K+M+  +R+PPKHFE  V+ HF  RA  +L ACKAYM+G  VGS     
Sbjct: 808 SYNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGSSA--- 867

Query: 729 VQDLDLGDKSCSNHFKQSLYQHLRMLVETFTQLGVSDCEKFLPP 771
             +L     + S  FK  L +    L+E F+++GV DC + + P
Sbjct: 868 --NLQGNSTTNSTGFKIMLSKLYPKLLEAFSEIGV-DCVQEIGP 905

BLAST of Sgr028190.1 vs. TAIR 10
Match: AT2G16920.1 (ubiquitin-conjugating enzyme 23 )

HSP 1 Score: 297.0 bits (759), Expect = 4.7e-80
Identity = 147/275 (53.45%), Postives = 185/275 (67.27%), Query Frame = 0

Query: 494  FKQFDTVEDHSDHRYTSSGFTMKQLPRKWTKRIQEEWKILEKDLPETIFVRTYESRMDLM 553
            F++FD  +D  DH +       K   R+W K++ ++WKIL+ +LP+ IFVR YE RMDL+
Sbjct: 824  FRRFDISQDPLDHHFLGVD-GQKTKERQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDLL 883

Query: 554  RAVIIGAEGTPYHDGLFFFDIFFPPSYPDVPPQVYYHSGGLRLNPNLYNCGKVCLSLLNT 613
            RAVI+GA GTPY DGLFFFD   P  YP VPP  YYHSGG RLNPNLY  GKVCLSLLNT
Sbjct: 884  RAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLYEEGKVCLSLLNT 943

Query: 614  WSGSGNERWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSVAGENKSQHYNEEAY 673
            W+G GNE W P  S++LQVLVS+QGL+LN+KPYFNE GY  Q G+  GE  S  YNE  +
Sbjct: 944  WTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLGYNENTF 1003

Query: 674  ILSIKTMVYNIRRPPKHFEDFVRGHFCKRAHDILVACKAYMDGAQVGSLVKD-GVQDLDL 733
            +L+ KTM+Y +R+PPK FE+ ++ HF KR + IL AC AYM G  +GSL KD  V D   
Sbjct: 1004 LLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDAYMKGYLIGSLTKDASVIDERS 1063

Query: 734  GDKSCSNHFKQSLYQHLRMLVETFTQLGVSDCEKF 768
               S S  FK  L +    L    +++G +DC +F
Sbjct: 1064 SANSTSVGFKLMLAKIAPKLFSALSEVG-ADCNEF 1096