Sgr028056.1 (mRNA) Monk fruit (Qingpiguo) v1

BLAST of Sgr028056.1 vs. NCBI nr
Match: KAG5558650.1 (hypothetical protein RHGRI_008560 [Rhododendron griersonianum])

HSP 1 Score: 266.9 bits (681), Expect = 3.0e-67
Identity = 152/397 (38.29%), Postives = 215/397 (54.16%), Query Frame = 0

Query: 48  ITSAALKSSNWEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVA-----QNQKHLRLD 107
           + S +  S +W +LP  +LD I+ KL  L D+ RF  VCK W   A     Q Q+H  L 
Sbjct: 1   MASVSADSPDWASLPGHLLDSIVEKLIDLPDFIRFAVVCKPWFCAATMNNQQKQQHRGLL 60

Query: 108 PYDKQLPLLFIPNRFPGRKN-------KPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWL 167
            + KQ+P+L +P+  P   N       K  LY I    +YD +L   Y +R CGS  GWL
Sbjct: 61  AH-KQIPMLMVPSSDPKDNNSDGEGQVKRGLYSITRGELYDFQLSLPYKKRFCGSSHGWL 120

Query: 168 ATVDMHLALSFTHPFSGRTVKFPSITELDPE--DVGESYPYEYKFRKAIMSKDPNLFPND 227
           A V+ + A++  +PFS    +   IT   P   ++G++  YEY   K +++ DP L P+ 
Sbjct: 121 AAVEDNYAITLINPFS----RVSPITLPPPRKVELGKNREYEYYVDKVVLTADPYLSPDS 180

Query: 228 YLVIAIHYNYLRFSCIKPGGKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISE 287
           Y   AI+  +   + IK G KDW  I   +L   +D+I Y+   Y L     +  +    
Sbjct: 181 YQAAAIYSGFSLVAFIKSGDKDWTYISKDQLTLAADIIYYESHFYVLDYRHRLIKIKSFS 240

Query: 288 DFDDSIRSKMQVIIPNKMEFAQLSYLVESSQGELFNVRRFIKLG--KFKYKTWKFDIFKL 347
             +  I  +  +  PN+   AQ SYLVESS GEL  V+RF+K    + +Y TW F ++KL
Sbjct: 241 GTEQRIELEAIIKKPNRRRPAQKSYLVESSAGELLTVQRFLKENDHELQYVTWYFKVYKL 300

Query: 348 VKDDEEEGKMKWFEVKTLGEDALFLGDNYSMSILASNFPNCKSNCIYYTDNYNDDGYEGH 407
                 +    W E++ +G DALFLGDNYS S++A  FP C  N IYYTDNYN+      
Sbjct: 301 -NCSSSQSHPVWVEIENIGGDALFLGDNYSFSLVAFQFPGCLPNSIYYTDNYNEILGSKP 360

Query: 408 TGSFDMGIYNVDDKSFGTHYFMPPSHKDLPPPIWILP 429
            G  DMGI+ +++KSF  HY + PSH D+PPPIWI+P
Sbjct: 361 LGPRDMGIFLLEEKSFRMHYTLDPSHCDMPPPIWIVP 391

BLAST of Sgr028056.1 vs. NCBI nr
Match: TQD98408.1 (hypothetical protein C1H46_016009 [Malus baccata])

HSP 1 Score: 258.8 bits (660), Expect = 8.0e-65
Identity = 144/388 (37.11%), Postives = 225/388 (57.99%), Query Frame = 0

Query: 56  SNWEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRLDPYDKQLPLLFIPN 115
           S W  LP+D+L LI+ K+   SDY RFG VCKHW  VA  QKH RL  + +QLP+L IP+
Sbjct: 11  SGWAWLPSDLLHLIVEKVIPTSDYIRFGVVCKHWQSVALLQKHRRLKSHQQQLPMLIIPS 70

Query: 116 RFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWLATVDMHLALSFTHPFSGRTV 175
           +     ++  LY +        EL   YN RCCGS  GWLA V+ +  ++  +PF+ RT+
Sbjct: 71  K-DNINDRRGLYSVMHGKNCGLELYVPYNNRCCGSSHGWLACVNDNFGVNLFNPFTKRTI 130

Query: 176 KFPSITELDPEDVGE-SYPYEYKFRKAIMSKDPNLFPNDYLVIAI-HYNYLRFSCIKPGG 235
             P +T++ P+D+ + +Y  ++  +K ++S DP+L PNDY V+A+   N  R + IK G 
Sbjct: 131 LLPPVTKV-PQDIRKHAYRRDHYIKKVVLSADPSLCPNDYEVVALFDCNGTRIAHIKSGD 190

Query: 236 KDWIEIED--------KRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDSIRSKMQV 295
             W  I+         +++    DV+ ++G   A S  GG+ S++IS   D + +  + +
Sbjct: 191 NAWTHIDQTIRYFYGLQQIIGFDDVLYHRGQFLAASREGGVFSINISN--DSTHKPHVSI 250

Query: 296 IIPNKMEFAQLSYLVESSQGELFNVRRFIKLGKFK--YKTWKFDIFKLVKDDEEEGKMKW 355
           ++P        +YLVESSQG+L  VR+F  L   +   + + F +FKL          +W
Sbjct: 251 VLPIVPSIDDKAYLVESSQGDLLLVRKFQSLNYSECFIENFSFKVFKLFSASGGRTGPEW 310

Query: 356 F-EVKTLGEDALFLGDNYSMSILASNFPNCKSNCIYY-TDNYNDDGYEGHTGSFDMGIYN 415
             E++++G+DA F+GDN S+ + AS+FP C+ N IY+  D+     YE   GS DM ++N
Sbjct: 311 IEEIESIGDDAFFMGDNQSICVSASDFPGCQPNSIYFCIDDRVGVAYEEAQGSLDMAVFN 370

Query: 416 VDDKSFGTHYF-MPPSHKDLPPPIWILP 429
           ++++ FGT Y+ +  S K++PP IWILP
Sbjct: 371 LENRRFGTRYYCLSRSQKNMPPSIWILP 394

BLAST of Sgr028056.1 vs. NCBI nr
Match: XP_028965113.1 (F-box protein At2g26160-like [Malus domestica])

HSP 1 Score: 256.5 bits (654), Expect = 4.0e-64
Identity = 148/407 (36.36%), Postives = 219/407 (53.81%), Query Frame = 0

Query: 51  AALKSSNWEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRL-DPYDKQLP 110
           A+   + W  LP DIL LIL KL  +SDY RF +VCKHW  VA + K  RL      QLP
Sbjct: 4   ASTSVAGWAWLPDDILGLILEKLIPISDYIRFSAVCKHWKSVALHHKQHRLVKSRHNQLP 63

Query: 111 LLFIPNRFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWLATVDMHLALSFTHP 170
           +L +P +    K +  LY +     Y  EL   Y++RCCGS  GWLA VD +L ++  +P
Sbjct: 64  MLMVPTKDSSNKRR-SLYSVTRGKTYGFELHVPYSKRCCGSSHGWLACVDDNLVVTLLNP 123

Query: 171 FSGRTVKFPSITELDPEDVGESYPYEYKFRKAIMSKDPNLFPNDYLVIAIHYNY-LRFSC 230
           F+GRT+  P +  +       ++  +Y   K ++S +P+LFPNDY  + I+  Y  R + 
Sbjct: 124 FTGRTISLPPV-PVSASRARAAFRCDYYINKVVLSANPSLFPNDYEAVVIYDGYGKRIAH 183

Query: 231 IKPGGKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDSIRSKMQVIIP 290
           IK G + W  I+  ++    DVI YKG   A+S  G + S+++S   D + +  + V++P
Sbjct: 184 IKSGDEAWTCID--QVIGFDDVIYYKGQFLAVSLDGSVFSINVSS--DQTAKPHVSVVVP 243

Query: 291 NKMEFAQLSYLVESSQGELFNVR---------RFIKLGKFKY------------------ 350
                   + L ESS+G+L  +R         RF+K   FK+                  
Sbjct: 244 MSPGTDNKTCLAESSRGDLLLIRKFRRLNYCKRFMKSLSFKFFKFEKLEFEQVNGYDRFT 303

Query: 351 KTWKFDIFKLVKDDEEEGKMKWFEVKTLGEDALFLGDNYSMSILASNFPNCKSNCIYYTD 410
           +T  F +FKL    E+  + +W EV+++G DALFLG N SM + AS+F  C+ N IY+TD
Sbjct: 304 ETLSFKVFKLFSSYED--RPQWVEVESIGNDALFLGTNQSMCVSASDFQGCRPNSIYFTD 363

Query: 411 NYNDDGYEGHTGSFDMGIYNVDDKSFGTHYFMPPSHKDLPPPIWILP 429
           +  D       G  DMG++++++KS G HY +  S K +PP IWILP
Sbjct: 364 DCVDVACHKPKGPHDMGVFDIENKSMGRHYCLDRSQKHMPPAIWILP 402

BLAST of Sgr028056.1 vs. NCBI nr
Match: KAB2601684.1 (F-box protein [Pyrus ussuriensis x Pyrus communis])

HSP 1 Score: 256.1 bits (653), Expect = 5.2e-64
Identity = 147/410 (35.85%), Postives = 219/410 (53.41%), Query Frame = 0

Query: 51  AALKSSNWEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRL-DPYDKQLP 110
           A+   + W  LPAD+L LIL KL  +SDY RF +VCKHW   A + K  RL      QLP
Sbjct: 4   ASTSVAGWAWLPADVLGLILEKLIPISDYIRFTAVCKHWKSAALHHKQHRLVKSRPNQLP 63

Query: 111 LLFIPNRFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWLATVDMHLALSFTHP 170
           +L +P +    K +  LY +     Y  EL   Y++RCCGS  GWLA VD +L ++  +P
Sbjct: 64  MLMVPTKDSSNKGR-SLYSVTRGKTYGFELHVPYSKRCCGSSHGWLACVDENLVVTLLNP 123

Query: 171 FSGRTVKFPSITELDPEDVGESYPY---EYKFRKAIMSKDPNLFPNDYLVIAIHYNY-LR 230
           F+GRT+  P +    P     +  +   +Y   K ++S +P+LFPNDY  + I+  Y  R
Sbjct: 124 FTGRTISLPPV----PASASRARAFFRCDYYINKVVLSANPSLFPNDYEAVVIYDGYGKR 183

Query: 231 FSCIKPGGKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDSIRSKMQV 290
            + IK G + W  I+  ++    DVI YKG   A+S  G + S+++S   D + +  + V
Sbjct: 184 IAHIKSGDEAWTCID--QVIGFDDVIYYKGQFLAVSLDGSVFSINVSS--DQTAKPHVSV 243

Query: 291 IIPNKMEFAQLSYLVESSQGELFNVR---------RFIKLGKFKY--------------- 350
           ++P        +YL +SS+G+L  +R         RF+K   FK+               
Sbjct: 244 VVPMSPGTDNKTYLAQSSRGDLLLIRKFQRLTYCKRFVKSLSFKFFKFEKLEFEQVNGYD 303

Query: 351 ---KTWKFDIFKLVKDDEEEGKMKWFEVKTLGEDALFLGDNYSMSILASNFPNCKSNCIY 410
              +T  F +FKL    E+  + +W EV+++G DALFLG N SM + AS+F  C+ N IY
Sbjct: 304 RFTETLSFKVFKLFSSYED--RPQWVEVESIGNDALFLGTNQSMCVSASHFQGCRPNSIY 363

Query: 411 YTDNYNDDGYEGHTGSFDMGIYNVDDKSFGTHYFMPPSHKDLPPPIWILP 429
           + D+  D       G  DMG++++++KS G HY +  S K +PP IWILP
Sbjct: 364 FADDCVDVACHKPKGPHDMGVFDIENKSMGRHYCLDRSQKHMPPAIWILP 402

BLAST of Sgr028056.1 vs. NCBI nr
Match: XP_009354047.1 (PREDICTED: F-box protein At2g26160-like [Pyrus x bretschneideri])

HSP 1 Score: 255.8 bits (652), Expect = 6.8e-64
Identity = 148/415 (35.66%), Postives = 220/415 (53.01%), Query Frame = 0

Query: 46  SNITSAALKSSNWEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRL-DPY 105
           S +  A+   + W  LPAD+L LIL KL  +SDY RF +VCKHW   A + K  RL    
Sbjct: 11  STMEEASTSVAGWAWLPADVLGLILEKLIPISDYIRFTAVCKHWKSAALHHKQHRLVKSR 70

Query: 106 DKQLPLLFIPNRFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWLATVDMHLAL 165
             QLP+L +P +    K +  LY +     Y  EL   Y++RCCGS  GWLA VD +L +
Sbjct: 71  PNQLPMLMVPTKDSSNKRR-SLYSVTRGKTYGFELHVPYSKRCCGSSHGWLACVDDNLVV 130

Query: 166 SFTHPFSGRTVKFPSITELDPEDVGESYPY---EYKFRKAIMSKDPNLFPNDYLVIAIHY 225
           +  +PF+GRT+  P +    P     +  +   +Y   K ++S +P+LFPNDY  + I+ 
Sbjct: 131 TLLNPFTGRTISLPPV----PASASRARAFFRCDYYINKVVLSANPSLFPNDYEAVVIYD 190

Query: 226 NY-LRFSCIKPGGKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDSIR 285
            Y  R + IK G + W  I+  ++    DVI YKG   A+S  G + S+++S   D + +
Sbjct: 191 GYGKRIAHIKSGDEAWTCID--QVIGFDDVIYYKGQFLAVSLDGSVYSINVSS--DQTAK 250

Query: 286 SKMQVIIPNKMEFAQLSYLVESSQGELFNVR---------RFIKLGKFKY---------- 345
             + V++P        +YL +SS+G+L  +R         RF+K   FK+          
Sbjct: 251 PHVSVVVPMSPGTDNKTYLAQSSRGDLLLIRKFQRLTYCKRFVKSLSFKFFNFEKLEFEQ 310

Query: 346 --------KTWKFDIFKLVKDDEEEGKMKWFEVKTLGEDALFLGDNYSMSILASNFPNCK 405
                   +T  F +FKL    E+  + +W EV+++G DALFLG N SM + AS+F  C+
Sbjct: 311 VNGYDRFTETLSFKVFKLFSSYED--RPQWVEVESIGNDALFLGTNQSMCVSASHFQGCR 370

Query: 406 SNCIYYTDNYNDDGYEGHTGSFDMGIYNVDDKSFGTHYFMPPSHKDLPPPIWILP 429
            N IY+ D+  D       G  DMG++++++KS G HY    S K +PP IWILP
Sbjct: 371 PNSIYFADDCVDVACHKPKGPHDMGVFDIENKSMGRHYCWDRSQKHMPPAIWILP 414

BLAST of Sgr028056.1 vs. ExPASy Swiss-Prot
Match: B3H6C3 (F-box protein At4g35733 OS=Arabidopsis thaliana OX=3702 GN=At4g35733 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 3.1e-19
Identity = 100/380 (26.32%), Postives = 163/380 (42.89%), Query Frame = 0

Query: 58  WEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRLDPYDKQLPLLFIPNRF 117
           W  LP ++LD I N LF   +  RF S+CK +     + K+  LD   +    L  P   
Sbjct: 7   WSDLPGELLDHIANGLFSKVELLRFRSICKTFRSAVDSDKNF-LDHLKRNRRRLLSPY-- 66

Query: 118 PGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWLATV-DMHLALS---------FT 177
                K C   +  AA Y   L   Y  +      GWL  + D +++           F+
Sbjct: 67  --STGKTC--SLSPAAFYRVVLS-SYPDK------GWLIKLQDAYVSSQKQLLSPLSRFS 126

Query: 178 HPFSGRTVKFPSITELDPEDVGESYPYEYKF---RKAIMSKDPNLFPNDYLVIAIHYNYL 237
              SG+T+    + E    ++ +SY  EY +    +A  +    +   D++ I  +Y  +
Sbjct: 127 IKSSGKTL---DLLEFTVSEIHQSYDVEYLYYNSTRASFNFARVVLAEDFVFIVDNYKKI 186

Query: 238 RFSCIKPGGKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISE----DFDDSIR 297
                      W+ I D+ ++  SD++ +KG +YAL   G +  + +SE     F  S  
Sbjct: 187 WLCNSNESDSHWVRIMDEEVKLFSDIVFHKGYMYALDLTGAVWWISLSEFGIFQFGPSST 246

Query: 298 SKMQVIIPNKMEFAQLSYLVESSQGELFNVRRFIKLGKFKYKTWKFDI---FKLVKDDEE 357
                 I    +   + Y      G+L  V RF +  KF+ K    D+   FK+ K DEE
Sbjct: 247 PMDYCDIDECKDKRFVEYC-----GDLCIVHRFSR--KFRIKRVDIDMTVGFKVYKMDEE 306

Query: 358 EGKMKWFEVKTLGEDALFLGDNYSMSILASNFPNCKSNCIYYTDNYNDDGYEGHTGSFDM 416
              +++ EVK+LG+ A  +  +   S+LA  +  C  N IY+T+  N   ++   GS   
Sbjct: 307 --LVEYVEVKSLGDKAFVMATDSCFSVLAREYYGCLENSIYFTEQNNVKVFKLGDGS--- 356

BLAST of Sgr028056.1 vs. ExPASy Swiss-Prot
Match: Q9C6X9 (Probable F-box protein At1g44080 OS=Arabidopsis thaliana OX=3702 GN=At1g44080 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 7.2e-16
Identity = 95/364 (26.10%), Postives = 143/364 (39.29%), Query Frame = 0

Query: 58  WEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRLDPYDKQLPLLFIPNRF 117
           W  L  D++DL+ N L    +  RF S+CK W      +K L               N F
Sbjct: 6   WSDLHEDLIDLLANNLSSNINLLRFRSICKPWRSTVATKKRLH--------------NHF 65

Query: 118 PGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWL---ATVDMHLALSFTHPFSGRT 177
              +N P     KT     T  +      C     GWL     V      +   P SG+T
Sbjct: 66  --ERNLPTFKKKKTVVSPSTFFRVTLPSPCRNK--GWLIKNRQVSESSKNNLLSPLSGKT 125

Query: 178 VKFPSITELDPEDVGESYPYEYKFRKAIMSKDPNLFPNDYLVIAIHYNYLRFSCIKPG-- 237
           +  PS   LD   V E +  +    +     D  +F ++   +    N     C K G  
Sbjct: 126 IT-PSDKTLDLLKV-ECF-RDSSILQLFADSDRVVFLDNVFFVVDFKN--EIWCCKSGEE 185

Query: 238 GKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDSIRSKMQVIIPNKME 297
            + W  I ++  +   D+I++KG +YAL   G I  + +SE       S  Q      ++
Sbjct: 186 TRHWTRINNEEAKGFLDIILHKGKIYALDLTGAIWWISLSE------LSIYQYGPSTPVD 245

Query: 298 FAQLSYLVESSQ----GELFNVRRFIKLGKFKYK---TWKFDIFKLVKDDEEEGKMKWFE 357
           F ++    E       GEL  V RF K  KF  K   T +   FK+ K D  +  ++W E
Sbjct: 246 FYEIDNCKEKRLVEYCGELCVVHRFYK--KFCVKRVLTERTVCFKVYKMD--KNLVEWVE 305

Query: 358 VKTLGEDALFLGDNYSMSILASNFPNCKSNCIYYTDNYNDDGYEGHTGSFDMGIYNVDDK 410
           V +LG+ AL +  +    +LAS +  C  N IY+ D              D+ ++ +DD 
Sbjct: 306 VSSLGDKALIVATDNCFLVLASEYYGCLENAIYFNDRE------------DVSVFKLDDC 324

BLAST of Sgr028056.1 vs. ExPASy Swiss-Prot
Match: Q9LS04 (Putative F-box protein At3g25750 OS=Arabidopsis thaliana OX=3702 GN=At3g25750 PE=4 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 2.3e-14
Identity = 77/346 (22.25%), Postives = 142/346 (41.04%), Query Frame = 0

Query: 53  LKSSNWEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRLDPYDKQLPLLF 112
           ++ + W  LP ++LDLI N+     D  R  S CK W       K      +++ LP   
Sbjct: 1   MEKTEWSDLPEELLDLIANRYSSNIDVLRIRSTCKSWRSAVAMSKERLQFRFERYLP--- 60

Query: 113 IPNRFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSC--FGWLATV----DMHLALSF 172
                    NK     +     +   L          SC   GWL        M+  ++ 
Sbjct: 61  -------TSNKKIKAHLSPTTFFRITLP--------SSCPNKGWLVRTRQASKMYRKITL 120

Query: 173 THPFSG----RTVKFPSITELDPEDVGESYPYE----YKFRKAIMSKD--PNLFPNDYLV 232
             P SG    R+ +   + ++   ++ +SY  +     K  K  +  +   N   N   +
Sbjct: 121 LCPLSGERITRSHQTLDLLKVGVSEIRQSYEIQIFDGLKDEKIPLDSEIFSNYIKNSDKI 180

Query: 233 IAIHYNYLRFSCIK--PGGKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISED 292
            +++Y+  +  C K   G + W +I++ ++   SD+I++ G +YA+   G I  + +S+ 
Sbjct: 181 PSVNYHDNKIWCCKTREGSRSWTKIKN-QVEDFSDIILHMGRIYAVDLKGAIWWISLSQ- 240

Query: 293 FDDSIRSKMQVIIPNKMEFAQLSYLVESSQGELFNVRRFIKLGKFKYKTWKFDIFKLVKD 352
                ++    +   K +  Q + LVE   G+L  V           +T  F ++K+   
Sbjct: 241 LTIVQQTSSTPLDYYKYDSCQDTRLVEYC-GDLCIVHELSITRNHIQRTVGFKVYKM--- 300

Query: 353 DEEEGKMKWFEVKTLGEDALFLGDNYSMSILASNFPNCKSNCIYYT 381
             +E   KW EV  LG++ L +  N   +++AS +  C  N IY++
Sbjct: 301 --DEDLAKWVEVSCLGDNTLIVACNSCFTVVASEYHGCLKNSIYFS 320

BLAST of Sgr028056.1 vs. ExPASy Swiss-Prot
Match: Q9SHX9 (Putative F-box protein At1g65770 OS=Arabidopsis thaliana OX=3702 GN=At1g65770 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 8.8e-14
Identity = 92/353 (26.06%), Postives = 152/353 (43.06%), Query Frame = 0

Query: 56  SNWEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRLDPYDKQLPLLFIPN 115
           ++W  LP D+L++I  +LF   +  RF S+C+ W          + +P+  +  +L  PN
Sbjct: 2   ADWSTLPVDLLNMIAGRLFSNIELKRFRSICRSWRSSVPGAG--KKNPFRTRPLILLNPN 61

Query: 116 RFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWLATVDMHLALSFTH---PFS- 175
                 NKP L D +    + +   +           GWL   D+ ++    H   P S 
Sbjct: 62  -----PNKP-LTDHRRRGEFLSRSAFFRVTLSSSPSQGWLIKSDVDVSSGKLHLLDPLSR 121

Query: 176 ---GRTVKFPSITELDPEDVGESYP-YEYK--------FRKAIMSKDPNLFPNDYLVIAI 235
                + K   ++E    ++ E+Y  ++++        F++  + KD      D  V+ I
Sbjct: 122 LPMEHSRKRVDLSEFTITEIREAYQVHDWRTRKETRPIFKRVALVKDKE---GDNQVLGI 181

Query: 236 H-YNYLRFSCIKPGGKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDS 295
                + +  I    K W   E+      SD+I++KG  YAL S G +            
Sbjct: 182 RSTGKMMYWDI----KTWKAKEEG--YEFSDIIVHKGQTYALDSIGIV----------YW 241

Query: 296 IRSKMQVII--PNKMEFAQLSYLVESSQGELFNVRRFIKLGKFK-------YKTWKFDIF 355
           IRS ++ I   P   ++     LVE   GE + V R +    +K       Y+  K   F
Sbjct: 242 IRSDLKFIRFGPLVGDWTGDRRLVECC-GEFYIVERLVGESTWKRKADDTGYEYAKTVGF 301

Query: 356 KLVKDDEEEGKMKWFEVKTLGEDALFLGDNYSMSILASNFPNCKSNCIYYTDN 383
           K+ K D+E+GKM   EVK+LG+ A  +  +   S+LA  F  C  N IY+TD+
Sbjct: 302 KVYKFDDEQGKM--MEVKSLGDKAFVIATDTCFSVLAHEFYGCLENAIYFTDD 324

BLAST of Sgr028056.1 vs. ExPASy Swiss-Prot
Match: Q9LVG8 (Putative F-box protein At5g60060 OS=Arabidopsis thaliana OX=3702 GN=At5g60060 PE=4 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.9e-08
Identity = 96/408 (23.53%), Postives = 163/408 (39.95%), Query Frame = 0

Query: 50  SAALKSSNWEALPADILDLILNKLFRLSD----YYRFGSVCKHW-LYVAQNQKHLRLDPY 109
           S++L  S W  LP DIL+LI ++L   S          SVC  W L +  + K+ RL  +
Sbjct: 3   SSSLLPSQWSDLPLDILELISDRLDHDSSDTIHLLCLRSVCATWRLSLPLSNKNNRLSKF 62

Query: 110 DKQLPLLFIPNRFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCC-----GSCFGWLATVD 169
            K LP       +    +    + +K + +Y  +L+   N R C      +  G +  +D
Sbjct: 63  PKYLPF------WSSSSSSSGFFTLKQSNVY--KLEAPLNPRTCLVKLQETTPGIMRVLD 122

Query: 170 MHLALSFTHPFSGRTV-----KFPS---ITELDPEDVGESYPYEY--------------- 229
           +         FS   +      FPS   + E     V  +Y  EY               
Sbjct: 123 L---------FSNDRICFLPENFPSKIDLQEFHVRLVRRTYRMEYANNGGGAVPCFWSLH 182

Query: 230 KFRKAIMSKDPNLFPNDYLVIAIHYNYLRFSCIKPGGKDWIEIEDKRLRPL-SDVIIYKG 289
             +  I+S        D  +IAIH    +   +K G  +  +I D     +  D+++YK 
Sbjct: 183 SDKVVILSSG-----EDSAIIAIHSGG-KLGFLKSGNDEKWKILDNSWNVIYEDIMLYKD 242

Query: 290 LVYALSSYGGIGSLDISEDFDDSIRSKMQVIIPNKMEFAQLSYLVESSQGELFNVRRFIK 349
               +    G     +  D D  +    + ++          +LVE S GE+F V +++K
Sbjct: 243 NRCIVVDDKG---KTVIYDVDFKVSDLAEGLVGGG---GHKKHLVECSGGEVFLVDKYVK 302

Query: 350 L----GKFKYKTWKFDIFKLVKDDEEEGKMKWFEVKTLGEDALFLGDNYSMSILASNFPN 409
                        +F ++ L ++++     +W EV+ LG+ ALF+GD+ S S+  +   +
Sbjct: 303 TVWCKSDISKSVVEFRVYNLKREEK-----RWEEVRDLGDVALFIGDDCSFSV-QNPAGD 362

Query: 410 CKSNCIYYTDNYNDDGYEGHTGSFDMGIYNVDDK-----SFGTHYFMP 415
                I+Y+D  N     G     D G++NVD +     S   +YF P
Sbjct: 363 LAGGFIFYSDYRNGGRSRGICSDGD-GVFNVDMQGDFVFSIKPNYFGP 374

BLAST of Sgr028056.1 vs. ExPASy TrEMBL
Match: A0A540MI13 (DUF295 domain-containing protein OS=Malus baccata OX=106549 GN=C1H46_016009 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 3.9e-65
Identity = 144/388 (37.11%), Postives = 225/388 (57.99%), Query Frame = 0

Query: 56  SNWEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRLDPYDKQLPLLFIPN 115
           S W  LP+D+L LI+ K+   SDY RFG VCKHW  VA  QKH RL  + +QLP+L IP+
Sbjct: 11  SGWAWLPSDLLHLIVEKVIPTSDYIRFGVVCKHWQSVALLQKHRRLKSHQQQLPMLIIPS 70

Query: 116 RFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWLATVDMHLALSFTHPFSGRTV 175
           +     ++  LY +        EL   YN RCCGS  GWLA V+ +  ++  +PF+ RT+
Sbjct: 71  K-DNINDRRGLYSVMHGKNCGLELYVPYNNRCCGSSHGWLACVNDNFGVNLFNPFTKRTI 130

Query: 176 KFPSITELDPEDVGE-SYPYEYKFRKAIMSKDPNLFPNDYLVIAI-HYNYLRFSCIKPGG 235
             P +T++ P+D+ + +Y  ++  +K ++S DP+L PNDY V+A+   N  R + IK G 
Sbjct: 131 LLPPVTKV-PQDIRKHAYRRDHYIKKVVLSADPSLCPNDYEVVALFDCNGTRIAHIKSGD 190

Query: 236 KDWIEIED--------KRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDSIRSKMQV 295
             W  I+         +++    DV+ ++G   A S  GG+ S++IS   D + +  + +
Sbjct: 191 NAWTHIDQTIRYFYGLQQIIGFDDVLYHRGQFLAASREGGVFSINISN--DSTHKPHVSI 250

Query: 296 IIPNKMEFAQLSYLVESSQGELFNVRRFIKLGKFK--YKTWKFDIFKLVKDDEEEGKMKW 355
           ++P        +YLVESSQG+L  VR+F  L   +   + + F +FKL          +W
Sbjct: 251 VLPIVPSIDDKAYLVESSQGDLLLVRKFQSLNYSECFIENFSFKVFKLFSASGGRTGPEW 310

Query: 356 F-EVKTLGEDALFLGDNYSMSILASNFPNCKSNCIYY-TDNYNDDGYEGHTGSFDMGIYN 415
             E++++G+DA F+GDN S+ + AS+FP C+ N IY+  D+     YE   GS DM ++N
Sbjct: 311 IEEIESIGDDAFFMGDNQSICVSASDFPGCQPNSIYFCIDDRVGVAYEEAQGSLDMAVFN 370

Query: 416 VDDKSFGTHYF-MPPSHKDLPPPIWILP 429
           ++++ FGT Y+ +  S K++PP IWILP
Sbjct: 371 LENRRFGTRYYCLSRSQKNMPPSIWILP 394

BLAST of Sgr028056.1 vs. ExPASy TrEMBL
Match: A0A5N5FTS4 (F-box protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_002689 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 2.5e-64
Identity = 147/410 (35.85%), Postives = 219/410 (53.41%), Query Frame = 0

Query: 51  AALKSSNWEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRL-DPYDKQLP 110
           A+   + W  LPAD+L LIL KL  +SDY RF +VCKHW   A + K  RL      QLP
Sbjct: 4   ASTSVAGWAWLPADVLGLILEKLIPISDYIRFTAVCKHWKSAALHHKQHRLVKSRPNQLP 63

Query: 111 LLFIPNRFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWLATVDMHLALSFTHP 170
           +L +P +    K +  LY +     Y  EL   Y++RCCGS  GWLA VD +L ++  +P
Sbjct: 64  MLMVPTKDSSNKGR-SLYSVTRGKTYGFELHVPYSKRCCGSSHGWLACVDENLVVTLLNP 123

Query: 171 FSGRTVKFPSITELDPEDVGESYPY---EYKFRKAIMSKDPNLFPNDYLVIAIHYNY-LR 230
           F+GRT+  P +    P     +  +   +Y   K ++S +P+LFPNDY  + I+  Y  R
Sbjct: 124 FTGRTISLPPV----PASASRARAFFRCDYYINKVVLSANPSLFPNDYEAVVIYDGYGKR 183

Query: 231 FSCIKPGGKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDSIRSKMQV 290
            + IK G + W  I+  ++    DVI YKG   A+S  G + S+++S   D + +  + V
Sbjct: 184 IAHIKSGDEAWTCID--QVIGFDDVIYYKGQFLAVSLDGSVFSINVSS--DQTAKPHVSV 243

Query: 291 IIPNKMEFAQLSYLVESSQGELFNVR---------RFIKLGKFKY--------------- 350
           ++P        +YL +SS+G+L  +R         RF+K   FK+               
Sbjct: 244 VVPMSPGTDNKTYLAQSSRGDLLLIRKFQRLTYCKRFVKSLSFKFFKFEKLEFEQVNGYD 303

Query: 351 ---KTWKFDIFKLVKDDEEEGKMKWFEVKTLGEDALFLGDNYSMSILASNFPNCKSNCIY 410
              +T  F +FKL    E+  + +W EV+++G DALFLG N SM + AS+F  C+ N IY
Sbjct: 304 RFTETLSFKVFKLFSSYED--RPQWVEVESIGNDALFLGTNQSMCVSASHFQGCRPNSIY 363

Query: 411 YTDNYNDDGYEGHTGSFDMGIYNVDDKSFGTHYFMPPSHKDLPPPIWILP 429
           + D+  D       G  DMG++++++KS G HY +  S K +PP IWILP
Sbjct: 364 FADDCVDVACHKPKGPHDMGVFDIENKSMGRHYCLDRSQKHMPPAIWILP 402

BLAST of Sgr028056.1 vs. ExPASy TrEMBL
Match: A0A2N9GB60 (DUF295 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS24694 PE=4 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 3.3e-64
Identity = 137/379 (36.15%), Postives = 217/379 (57.26%), Query Frame = 0

Query: 56  SNWEALPADILDLILNKLFRLS-DYYRFGSVCKHW--LYVAQNQKHLRLDPYDKQLPLLF 115
           SNW  LP ++L+ IL+K+     D  RFG VCK W  + +   Q+  R+     Q+P+L 
Sbjct: 8   SNWAWLPRNLLNSILDKVASSPFDLVRFGVVCKPWHTVEIEYIQEKRRMKGQGNQVPMLL 67

Query: 116 IPNRFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWLATVDMHLALSFTHPFSG 175
           IP ++  ++ +  LY I    IY  +L+  YN+RCCGS FGWL+ V    +++  +P   
Sbjct: 68  IPTKYKSKRAR-SLYSITEQKIYHCKLRMPYNKRCCGSSFGWLSFVTDTFSITLFNPLKN 127

Query: 176 RTVKFPSITELDPEDVGESYPYEYKFRKAIMSKDPNLFPNDYLVIAIHYNYLRFSCIKPG 235
             +  P +T++  E +     Y+Y   K  +S DP   P + LV+AI+  Y + + +K G
Sbjct: 128 MKIHLPQLTKI-KEPIYLILNYQYSVYKLTLSSDPAKTPTNCLVVAIYGLYNKLAFMKLG 187

Query: 236 GKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDSIRSKMQVIIPNKME 295
            + W  ++++     SD++ +KG V AL  + G+ S+D++        +++ +++PN +E
Sbjct: 188 AESWTYVDEELNILFSDILFFKGQVLALGHHSGLVSIDVNS-------NEINMLVPNDLE 247

Query: 296 FAQLSYLVESSQGELFNVRRFI----KLGKFKYKTWKFDIFKLVKDDEEEGKMKWFEVKT 355
           +A  +YLV++  G+L  VRRF     +    +  T  F ++K V DDE    +   EVKT
Sbjct: 248 YAMQTYLVDTCSGDLLLVRRFFDPNNRYSSSQSLTGSFKVYKFVLDDESGLVLDRVEVKT 307

Query: 356 LGEDALFLGDNYSMSILASNFPNCKSNCIYYTDNYNDDGYEGHTGSFDMGIYNVDDKSFG 415
           +G++ALFLGDNYS+S+LA+ FP C+ N IYYTD+Y D       G+ DMGI+N+ D S  
Sbjct: 308 IGDNALFLGDNYSISVLATEFPGCQPNSIYYTDDYIDTKPYYPDGAVDMGIFNLMDGSIR 367

Query: 416 THYFMPPSHKDLPPPIWIL 428
            HY    S K +PPPIWI+
Sbjct: 368 QHYKPKSSIKRMPPPIWIM 377

BLAST of Sgr028056.1 vs. ExPASy TrEMBL
Match: A0A498JXM0 (Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_011038 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 4.3e-64
Identity = 144/388 (37.11%), Postives = 222/388 (57.22%), Query Frame = 0

Query: 56  SNWEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRLDPYDKQLPLLFIPN 115
           S W  LP+D+L LI+ KL   SDY RFG+VCKHW  VA  QK  RL  + +QLP+L IP+
Sbjct: 11  SGWAWLPSDLLHLIVEKLIPTSDYIRFGAVCKHWQSVALLQKQRRLKSHQQQLPMLIIPS 70

Query: 116 RFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWLATVDMHLALSFTHPFSGRTV 175
           +     ++  LY +        EL   YN RCCGS  GWLA V+ +  ++  +PF  RT+
Sbjct: 71  K-DNINDRRGLYSVMHGKNCGFELYVPYNNRCCGSSHGWLACVNDNFGVNLFNPFIKRTI 130

Query: 176 KFPSITELDPEDVGE-SYPYEYKFRKAIMSKDPNLFPNDYLVIAI-HYNYLRFSCIKPGG 235
             P +T++ P+D+ + +Y  ++  +K ++S DP+L PNDY V+A+   N  R + IK G 
Sbjct: 131 LLPPVTKV-PQDIRKHAYRRDHYIKKVVLSADPSLCPNDYEVVALFDCNGTRIAHIKSGD 190

Query: 236 KDWIEIED--------KRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDSIRSKMQV 295
             W  I+          ++    DV+ Y+G   A S  GG+ S++IS   D + +  + +
Sbjct: 191 NAWTHIDQTIRYFYGLHQIIGFDDVLYYRGQFLAASREGGVFSINISN--DSTHKPHVSI 250

Query: 296 IIPNKMEFAQLSYLVESSQGELFNVRRFIKLGKFK--YKTWKFDIFKLVKDDEEEGKMKW 355
           ++P        +YLVESSQG+L  VR+F  L   +   + + F +FKL          +W
Sbjct: 251 VLPIVPSIDDKAYLVESSQGDLLLVRKFQSLNYSECFIENFSFKVFKLFSASGGRTGPEW 310

Query: 356 F-EVKTLGEDALFLGDNYSMSILASNFPNCKSNCIYY-TDNYNDDGYEGHTGSFDMGIYN 415
             E++++G+DA F+GDN S+ + AS+FP C+ N IY+  D+     YE   GS DM ++N
Sbjct: 311 IEEIESIGDDAFFMGDNQSICVSASDFPGCQPNSIYFCIDDRVGVAYEEAQGSLDMAVFN 370

Query: 416 VDDKSFGTHYF-MPPSHKDLPPPIWILP 429
           ++++ FG  Y+ +  S K++PP IWILP
Sbjct: 371 LENRRFGRRYYCLSRSQKNMPPSIWILP 394

BLAST of Sgr028056.1 vs. ExPASy TrEMBL
Match: A0A2R6QVA4 (F-box protein OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc13164 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.3e-63
Identity = 153/394 (38.83%), Postives = 227/394 (57.61%), Query Frame = 0

Query: 55  SSNWEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLR--LDPYDKQLPLLF 114
           SS+W  LP ++LDLIL +L ++SDY RF +VCK WL VA  QKHLR   DP+ + +P+L 
Sbjct: 16  SSDWAKLPEELLDLILCRLIQVSDYLRFSAVCKPWLSVAAQQKHLRRLRDPHKQVVPMLL 75

Query: 115 IP--NRFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWLATVDMHLALSFTHPF 174
           +P        + +  LY          E+Q  Y RRC GS  GWLATV+    ++  +PF
Sbjct: 76  MPEVEEPDDNRGRRKLYSATQNKFLKLEIQVPYRRRCSGSSHGWLATVEEDFGITLFNPF 135

Query: 175 SGRTVKFPSITELDPEDVGES----YPYEYKFRKAIMSKDPNLFPNDYLVIAIHYNYLRF 234
           SG++++FP ++   P  V  +    + YEY+  K ++S DP+L P+ ++V AI+  + + 
Sbjct: 136 SGKSLRFPQVSP-QPNYVNPTKRHLHKYEYEVLKVVLSCDPSLEPDHFVVFAIYGVFRKL 195

Query: 235 SCIKPGGKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDSIRSKMQVI 294
           + IKPG K W  I+       +D I   G  Y +  Y G+ S+D+S +    +    +V 
Sbjct: 196 AYIKPGHKSWTYIQGGIY--FADAIYRLGKFYVMDCYSGVVSVDVSNNGTPQV----EVA 255

Query: 295 IPNKMEFAQLSYLVESSQ-GELFNVRRFIKLGKFKYK--TWKFDIFKLV-KDDEEEGKMK 354
             +       +Y+VESS  G+L  V+RF+       +  T  F IFKL+       G ++
Sbjct: 256 PLSAPTETSKAYIVESSSGGDLLLVQRFLAENDHSQQLVTSFFKIFKLLGLGGGGGGSVQ 315

Query: 355 WF----EVKTLGEDALFLGDNYSMSILASNFPNCKSNCIYYTDNYNDD--GYEGHTGSFD 414
            F    E+ +LG DALFLGDNYS+++ ASNFP C+ NCIYYTD+Y++D   YE + G  D
Sbjct: 316 QFAERVEIDSLGGDALFLGDNYSLAVSASNFPGCRPNCIYYTDDYDNDYMPYEPY-GPRD 375

Query: 415 MGIYNVDDKSFGTHYFMPPSHK--DLPPPIWILP 429
           MGIY+++DKSF  +Y +    K   +PPPIWI+P
Sbjct: 376 MGIYSLEDKSFTPYYQIDTKQKRMPMPPPIWIVP 401

BLAST of Sgr028056.1 vs. TAIR 10
Match: AT4G35733.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 97.8 bits (242), Expect = 2.2e-20
Identity = 100/380 (26.32%), Postives = 163/380 (42.89%), Query Frame = 0

Query: 58  WEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRLDPYDKQLPLLFIPNRF 117
           W  LP ++LD I N LF   +  RF S+CK +     + K+  LD   +    L  P   
Sbjct: 7   WSDLPGELLDHIANGLFSKVELLRFRSICKTFRSAVDSDKNF-LDHLKRNRRRLLSPY-- 66

Query: 118 PGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWLATV-DMHLALS---------FT 177
                K C   +  AA Y   L   Y  +      GWL  + D +++           F+
Sbjct: 67  --STGKTC--SLSPAAFYRVVLS-SYPDK------GWLIKLQDAYVSSQKQLLSPLSRFS 126

Query: 178 HPFSGRTVKFPSITELDPEDVGESYPYEYKF---RKAIMSKDPNLFPNDYLVIAIHYNYL 237
              SG+T+    + E    ++ +SY  EY +    +A  +    +   D++ I  +Y  +
Sbjct: 127 IKSSGKTL---DLLEFTVSEIHQSYDVEYLYYNSTRASFNFARVVLAEDFVFIVDNYKKI 186

Query: 238 RFSCIKPGGKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISE----DFDDSIR 297
                      W+ I D+ ++  SD++ +KG +YAL   G +  + +SE     F  S  
Sbjct: 187 WLCNSNESDSHWVRIMDEEVKLFSDIVFHKGYMYALDLTGAVWWISLSEFGIFQFGPSST 246

Query: 298 SKMQVIIPNKMEFAQLSYLVESSQGELFNVRRFIKLGKFKYKTWKFDI---FKLVKDDEE 357
                 I    +   + Y      G+L  V RF +  KF+ K    D+   FK+ K DEE
Sbjct: 247 PMDYCDIDECKDKRFVEYC-----GDLCIVHRFSR--KFRIKRVDIDMTVGFKVYKMDEE 306

Query: 358 EGKMKWFEVKTLGEDALFLGDNYSMSILASNFPNCKSNCIYYTDNYNDDGYEGHTGSFDM 416
              +++ EVK+LG+ A  +  +   S+LA  +  C  N IY+T+  N   ++   GS   
Sbjct: 307 --LVEYVEVKSLGDKAFVMATDSCFSVLAREYYGCLENSIYFTEQNNVKVFKLGDGS--- 356

BLAST of Sgr028056.1 vs. TAIR 10
Match: AT1G44080.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 86.7 bits (213), Expect = 5.1e-17
Identity = 95/364 (26.10%), Postives = 143/364 (39.29%), Query Frame = 0

Query: 58  WEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRLDPYDKQLPLLFIPNRF 117
           W  L  D++DL+ N L    +  RF S+CK W      +K L               N F
Sbjct: 6   WSDLHEDLIDLLANNLSSNINLLRFRSICKPWRSTVATKKRLH--------------NHF 65

Query: 118 PGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWL---ATVDMHLALSFTHPFSGRT 177
              +N P     KT     T  +      C     GWL     V      +   P SG+T
Sbjct: 66  --ERNLPTFKKKKTVVSPSTFFRVTLPSPCRNK--GWLIKNRQVSESSKNNLLSPLSGKT 125

Query: 178 VKFPSITELDPEDVGESYPYEYKFRKAIMSKDPNLFPNDYLVIAIHYNYLRFSCIKPG-- 237
           +  PS   LD   V E +  +    +     D  +F ++   +    N     C K G  
Sbjct: 126 IT-PSDKTLDLLKV-ECF-RDSSILQLFADSDRVVFLDNVFFVVDFKN--EIWCCKSGEE 185

Query: 238 GKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDSIRSKMQVIIPNKME 297
            + W  I ++  +   D+I++KG +YAL   G I  + +SE       S  Q      ++
Sbjct: 186 TRHWTRINNEEAKGFLDIILHKGKIYALDLTGAIWWISLSE------LSIYQYGPSTPVD 245

Query: 298 FAQLSYLVESSQ----GELFNVRRFIKLGKFKYK---TWKFDIFKLVKDDEEEGKMKWFE 357
           F ++    E       GEL  V RF K  KF  K   T +   FK+ K D  +  ++W E
Sbjct: 246 FYEIDNCKEKRLVEYCGELCVVHRFYK--KFCVKRVLTERTVCFKVYKMD--KNLVEWVE 305

Query: 358 VKTLGEDALFLGDNYSMSILASNFPNCKSNCIYYTDNYNDDGYEGHTGSFDMGIYNVDDK 410
           V +LG+ AL +  +    +LAS +  C  N IY+ D              D+ ++ +DD 
Sbjct: 306 VSSLGDKALIVATDNCFLVLASEYYGCLENAIYFNDRE------------DVSVFKLDDC 324

BLAST of Sgr028056.1 vs. TAIR 10
Match: AT1G65770.1 (ascorbic acid mannose pathway regulator 1 )

HSP 1 Score: 79.7 bits (195), Expect = 6.2e-15
Identity = 92/353 (26.06%), Postives = 152/353 (43.06%), Query Frame = 0

Query: 56  SNWEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRLDPYDKQLPLLFIPN 115
           ++W  LP D+L++I  +LF   +  RF S+C+ W          + +P+  +  +L  PN
Sbjct: 2   ADWSTLPVDLLNMIAGRLFSNIELKRFRSICRSWRSSVPGAG--KKNPFRTRPLILLNPN 61

Query: 116 RFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSCFGWLATVDMHLALSFTH---PFS- 175
                 NKP L D +    + +   +           GWL   D+ ++    H   P S 
Sbjct: 62  -----PNKP-LTDHRRRGEFLSRSAFFRVTLSSSPSQGWLIKSDVDVSSGKLHLLDPLSR 121

Query: 176 ---GRTVKFPSITELDPEDVGESYP-YEYK--------FRKAIMSKDPNLFPNDYLVIAI 235
                + K   ++E    ++ E+Y  ++++        F++  + KD      D  V+ I
Sbjct: 122 LPMEHSRKRVDLSEFTITEIREAYQVHDWRTRKETRPIFKRVALVKDKE---GDNQVLGI 181

Query: 236 H-YNYLRFSCIKPGGKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDS 295
                + +  I    K W   E+      SD+I++KG  YAL S G +            
Sbjct: 182 RSTGKMMYWDI----KTWKAKEEG--YEFSDIIVHKGQTYALDSIGIV----------YW 241

Query: 296 IRSKMQVII--PNKMEFAQLSYLVESSQGELFNVRRFIKLGKFK-------YKTWKFDIF 355
           IRS ++ I   P   ++     LVE   GE + V R +    +K       Y+  K   F
Sbjct: 242 IRSDLKFIRFGPLVGDWTGDRRLVECC-GEFYIVERLVGESTWKRKADDTGYEYAKTVGF 301

Query: 356 KLVKDDEEEGKMKWFEVKTLGEDALFLGDNYSMSILASNFPNCKSNCIYYTDN 383
           K+ K D+E+GKM   EVK+LG+ A  +  +   S+LA  F  C  N IY+TD+
Sbjct: 302 KVYKFDDEQGKM--MEVKSLGDKAFVIATDTCFSVLAHEFYGCLENAIYFTDD 324

BLAST of Sgr028056.1 vs. TAIR 10
Match: AT3G25750.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 79.0 bits (193), Expect = 1.1e-14
Identity = 83/339 (24.48%), Postives = 139/339 (41.00%), Query Frame = 0

Query: 53  LKSSNWEALPADILDLILNKLFRLSDYYRFGSVCKHWLYVAQNQKHLRLDPYDKQLPLLF 112
           ++ + W  LP ++LDLI N+     D  R  S CK W       K      +++ LP   
Sbjct: 1   MEKTEWSDLPEELLDLIANRYSSNIDVLRIRSTCKSWRSAVAMSKERLQFRFERYLP--- 60

Query: 113 IPNRFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCCGSC--FGWLATV----DMHLALSF 172
                    NK     +     +   L          SC   GWL        M+  ++ 
Sbjct: 61  -------TSNKKIKAHLSPTTFFRITLP--------SSCPNKGWLVRTRQASKMYRKITL 120

Query: 173 THPFSGRTVKFPSITELDPEDVGES---YPYEYKFRKAIMSKDPNLFPNDYLVIAIHYNY 232
             P SG  +   S   LD   VG S     YE +    +  KD  + P D  + +   NY
Sbjct: 121 LCPLSGERIT-RSHQTLDLLKVGVSEIRQSYEIQIFDGL--KDEKI-PLDSEIFS---NY 180

Query: 233 LRFSCIKP--GGKDWIEIEDKRLRPLSDVIIYKGLVYALSSYGGIGSLDISEDFDDSIRS 292
           ++ S   P  G + W +I++ ++   SD+I++ G +YA+   G I  + +S+      ++
Sbjct: 181 IKNSDKIPSEGSRSWTKIKN-QVEDFSDIILHMGRIYAVDLKGAIWWISLSQ-LTIVQQT 240

Query: 293 KMQVIIPNKMEFAQLSYLVESSQGELFNVRRFIKLGKFKYKTWKFDIFKLVKDDEEEGKM 352
               +   K +  Q + LVE   G+L  V           +T  F ++K+     +E   
Sbjct: 241 SSTPLDYYKYDSCQDTRLVEYC-GDLCIVHELSITRNHIQRTVGFKVYKM-----DEDLA 300

Query: 353 KWFEVKTLGEDALFLGDNYSMSILASNFPNCKSNCIYYT 381
           KW EV  LG++ L +  N   +++AS +  C  N IY++
Sbjct: 301 KWVEVSCLGDNTLIVACNSCFTVVASEYHGCLKNSIYFS 306

BLAST of Sgr028056.1 vs. TAIR 10
Match: AT5G60060.1 (Protein of unknown function (DUF295) )

HSP 1 Score: 62.0 bits (149), Expect = 1.3e-09
Identity = 96/408 (23.53%), Postives = 163/408 (39.95%), Query Frame = 0

Query: 50  SAALKSSNWEALPADILDLILNKLFRLSD----YYRFGSVCKHW-LYVAQNQKHLRLDPY 109
           S++L  S W  LP DIL+LI ++L   S          SVC  W L +  + K+ RL  +
Sbjct: 3   SSSLLPSQWSDLPLDILELISDRLDHDSSDTIHLLCLRSVCATWRLSLPLSNKNNRLSKF 62

Query: 110 DKQLPLLFIPNRFPGRKNKPCLYDIKTAAIYDTELQWDYNRRCC-----GSCFGWLATVD 169
            K LP       +    +    + +K + +Y  +L+   N R C      +  G +  +D
Sbjct: 63  PKYLPF------WSSSSSSSGFFTLKQSNVY--KLEAPLNPRTCLVKLQETTPGIMRVLD 122

Query: 170 MHLALSFTHPFSGRTV-----KFPS---ITELDPEDVGESYPYEY--------------- 229
           +         FS   +      FPS   + E     V  +Y  EY               
Sbjct: 123 L---------FSNDRICFLPENFPSKIDLQEFHVRLVRRTYRMEYANNGGGAVPCFWSLH 182

Query: 230 KFRKAIMSKDPNLFPNDYLVIAIHYNYLRFSCIKPGGKDWIEIEDKRLRPL-SDVIIYKG 289
             +  I+S        D  +IAIH    +   +K G  +  +I D     +  D+++YK 
Sbjct: 183 SDKVVILSSG-----EDSAIIAIHSGG-KLGFLKSGNDEKWKILDNSWNVIYEDIMLYKD 242

Query: 290 LVYALSSYGGIGSLDISEDFDDSIRSKMQVIIPNKMEFAQLSYLVESSQGELFNVRRFIK 349
               +    G     +  D D  +    + ++          +LVE S GE+F V +++K
Sbjct: 243 NRCIVVDDKG---KTVIYDVDFKVSDLAEGLVGGG---GHKKHLVECSGGEVFLVDKYVK 302

Query: 350 L----GKFKYKTWKFDIFKLVKDDEEEGKMKWFEVKTLGEDALFLGDNYSMSILASNFPN 409
                        +F ++ L ++++     +W EV+ LG+ ALF+GD+ S S+  +   +
Sbjct: 303 TVWCKSDISKSVVEFRVYNLKREEK-----RWEEVRDLGDVALFIGDDCSFSV-QNPAGD 362

Query: 410 CKSNCIYYTDNYNDDGYEGHTGSFDMGIYNVDDK-----SFGTHYFMP 415
                I+Y+D  N     G     D G++NVD +     S   +YF P
Sbjct: 363 LAGGFIFYSDYRNGGRSRGICSDGD-GVFNVDMQGDFVFSIKPNYFGP 374