Sgr025864.1 (mRNA) Monk fruit (Qingpiguo) v1

Overview
NameSgr025864.1
TypemRNA
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionAluminum-activated malate transporter
Locationtig00152985: 1848168 .. 1853352 (+)
Sequence length1407
RNA-Seq ExpressionSgr025864.1
SyntenySgr025864.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCGTACGGAGAGAAGATTTTTGATGGCAACAGTTAATGATCTTCTCCTGAACTTTTCTATAAAAGCAGAAACGGACGCCATGTCTGAAGCCTCAAGATCAGAGCTTCTCTCTCTCTCTTTCTTTTTCTCTCGCTTCTGCGGTGGTTATGGCGTCTGAGGGGTACGAAGGAGTGTTGGCTTATGTTGGGTGTGGGTGGGTGAAGAGGATATATGGGAATGTAGAAGATGAAGTGGTGGAGTTGGTGAGGAAGACGAAGAAGGTGGGGGAAGATGATCCGAGAAGGATTGTTCACTCTGTGAAAGTTGGTTTGGCCATCACGTTGGCGTCGTTGTTCTATTACTTCAAGCCTCTTTACGATGGATTTGGAGCCTCTGCAATTTGGACTGTTGTTACAGTCGTCGTTGTCTTCGAGTTCTCTGTTGGTACGTATTAATATCCTCCTTTAATCTACTCTCACTAGGCTCATTTCACCCTTCATATTATATTTTTTTTGTTAAATTATAAATTTAGTGTCTAGAATTTGAATACGGTATCTATTTAATCATTAAATTTTAAAATTTTTTAATTTCTTCATTTAACCTTAGATAATAGAAACTATCAATGTTGTATGCCCATTATCGACCGTATTATGCCAATTCAGCAAAAACTCATGTAATGGATATGCTATCTTAGCGTTTCGTTAATGATAGTAACAAAAAATAATTAAATAAAAGTATAATTTAAAGTTCAGAGATGTAATAAAAAAATTCTAAAGCTCAAAGACTAAATAGGCATCAGACTTAAAGTTGGGACAAAATTTATAATTTAACTTATATTTTTATGTAATCACAATTTTTTATAGGTCAGTTTGATAATGTTATTGTTCTCTTGTTTTTAAAATTATGCTTATTTATTTTCCTTTTCTTTTATTTGCTAATCTACTTTTGAAATAGTTATTGAAATCATAGAAAAATAGTTTTCAAAAACCTGTTCGCGGCAAAGATTTCAAAAGTATTTTTGCAAGGTGAAAAATAAATTTAATAAACATAAGTATAAGTAGATATTTTAAGGTTAAATTAAAAAAAAACTAAAAATAAAATAACATAGGTCTAAATTTTCATAATCATAATTTCTTAGTTATGAGATTCGTTATGTATATCAACTATAGATACTATAAAACATTTGAATATCTTTTAGTAACAATTTATTCTTGTATTTCAATGTTATTAACCATGCGTATTCAAGTATTTTATTCATAGTTTTGTATAACTATGAACTAAATACCTAAATTTGAGTACTTCTCTAGATATTAGTGATGAAAATGATAATTTACTTATAAGTGTTGCAGGATATATCAAGTATATATTGCATAAATAGTCCAATTTGAGTGATTATTCGTGTATATATTAATGATGTAAGTTTTAATTTTTAATTCAAAATTTTAGTAATTAATACAATTTCCAAGAATATATAAAATTAATTTTTATATTTCATAGAATAATTAGTGATATTATGTTATTTTCTATAGTATCATTGAATAGTTACATTATCTAGTGATTAAATATTTTTTTATACGTGTTCTCTTTCCATTATAAATGATAATTATGACATCATATGGCTTTTAAGTCTTTTTACACATTCAACACACCTGTCCATCCGAATATAGTTCAGTGGATAAAGACACTCTATGACCTTTCTTGTGGTGGAAGGTTCAACCTCACCCCTGCATTTGTTGAATTCAAATATATATATAAAATAAAAAAAACTAATATTATATGTGCTAATTTGGTAATATTTCCCTTATTTTGTCACAGGTGCGACACTTGGAAAGGGTTTGAATAGAGTGATGGCAACTTTACTAGGAGGGGCCCTAGGATTTGGAACTCATTATTTGGCAAGCTTATGTGGAGATAAAGGCCAGCCTGTAATGCTTGGGGCCATTGTTTTTATAGTAGGTATAAATTCATAGCTTAACCTAAATAGTTATGAATATAGTATTTTTCACAAAGTACCCAAATTGGTAATTATGCGAGAGACCAAATTGATAATTTAATAAAAAAAAACCCCACCTTTTGGTAGGATATTGAAACCCATAATTGAGATGAACCCAAGTAATTAAACAAACATGAACATAAAATACCAACGAGAAACCCTAAGTTATACTAGATTTGCAATAAAATATTTCTCTCCTTAAATTATAAAAATTTGGTCCCTAAATTTATAGGGTCCAATAAATTCTTGTTCTTTCAAGATTGTGTTTTATTTTTTTTAGAAAATATCATATCACAAATGCAAAGGTGGAGATTCAAACTTACAAATTCTTATAAATAGGGACGTTGACCACTGAGTTATGCTCTATTGGCAAGATGTCCATTTAGTTCCTATAGTTTAGAAATATTTTAATAGGCCTTTGAACTTTTAATTTTGTATCTAATATGTCTCGAACGTTAACTCTGTCAGTTTAACGCTTATGACATCAAATGCTTGAACCTACCTAAAATTAGTTTACCATGAAATATTTAAATAATACAATCAATGTGGCCAATAAACATTAAACTGATGGAATTAATAGCAAAATTCTATGAAAGTTTTAAAACCAAACTTGTAATTCTTTTTATTTTATTTTTTTGTAAGTTTTAAGTGATTTATTTTTGTTGATCTCGACAAACAGCTGCAACAGCAACTTTCATGCGATTCTTCCCAAATATGAAGGCGAGATACGACTATGGGCTTCTAATATTCATCTTGACGTTTTCAATGGTGGCTGTGTCCAGTTACAGAGATGATGAGATTCTAAAACTTGCTTTTGATAGGTTTCTGACCATTGTCATTGGAAGCTTCATCACTGCCTTCATTTGCATTTTTGTGTGGCCAATTTGGATTGGTAATGATCTTCACAACCTGCTTGTCACCAACATTCAAAACCTTGCCACCTTCTTACAAGGTACAAAAACAATCTCTCTTTGCTTCTTTATGTGCTAAATTTTAAATTAGCTTTCTTATCATTGACAGTAAATCAATATGATAATAACACAATACAATGACTATAACGAAACAGATTACGTATAGGAACTAGAATATTAATTAATGAGATGTTTGGCTTACGATTTTAATAAGTTTGAAAATTTTTGAAAAGCTCAAATTTATATAATCTGTATTAAGATTATACAAAAAAAACAAGTTATAGAAACTTGAATTTTTGTAAACTGTATGTTTGATTTATAAATTTATAATTTGTAAAAATTATAATATGGTATAAAATGCTTTATTTTATATGCATGAGTTTATATAGCTCATGGAATGAGAAAATCCCATAACTTAGAATAAGTAAATCTACATGTTAAACATAGAATTTTGCTTTTTTTTAAGCTTTGAATTAAACAAACTTAATCATGATTCAAATACTAAAGGGAATAGAATTATAATTTGACCAAAATAATAAAACAAATGAGAATTTAAACAACATTATTTTGTAAAACTAAAGTCAAGCAAACAGGAGATTAATTAATCTTCTCATAATCTTTTGTTAGCATATTCCATTTCTCTCTTCTTCCTTTATTTGATTGCTAGGTCTAACTTAAAATGTGGTGAAACATGCAGAGTTTGGAGTTGGATATTTCAGATTATCCCGAGAAGGAGCTGAGTTTAACAAAGCCAGCATGCTAAAATATAGAAGTATTCTAACTTCAAAACAAAGTGAAGAATCCATGGTGAGTCAAAATTAATATGAACCCAGCTCAAAATGATCTTCAGCCATTTCCCTCCAACGCTCGACTAAATATACACATGCTTTATCCAGGCTAACCTGGCTAGATGGGAGCCTTGGCATGGCAAGTTCAGATGTTGCCATCCCTGGAAACAGTACTTGAAGATCAGTTGCTCGGCTCGGGAATGCGCCTATCGGCTTCAAGTTCTTAATGGCTACCTAAACATTGATGATCAAAGAAGTCAGGTAAGAATGAGTTTATGATAACTTTTGAGAGTATTATTTTTAAGTGAAATACTTTTTAAAAAAAAACACTTTAAAAAAAAGAACTAAGAGTGAGTTTATGACTTTTGAGAATAGTATTTTTAAGTTAAAACACTTTTTTATAAGTACTTTCGAACAAAAACACTAATTAAGTATTTTTTTAAAAGTGCTTTTAAATGTTTTTAGAACTCTTAACAGTACTTATTTGACTATGTAAGCAAACACTATAGAAGATTTGAAAAGTGACTTTAGTTAGCTAAAAATACTTTTCAACTCTGTCAAGAGTCACGCCGAACTCACTTTTTTTGGTATAGCTTTTGAAATGGTGAAAAGTGTTTTAAGCCTATTTAAAATATTTTTCAAAATGTTCATGGTGTTTAGTTTCCAAAATGATTTTAAAATGGGTATGTGAGTTGTTGTAGCTTATTTCATTTCTTTTTTTTATTATTTTATTATTTTTTATGTTATCACTTTTGTAAATTTTAAAATGGGCTATCATTTATCCAAAACATCTACTCTTCACTAAATCTTTTCTAAAAGGTAAAAAATTAATTTTCAAAATAGCCCACTCCTTAAATTCTTGCAAAAATAAAACAATTTTAAATGAAATAACATTGATTTGGATATCTAGATCATCATTATTTATATATCTGCTAGAAGAGTAGATTTGTCTACACATAATTTTCCTTTCTTCCCAGTTTGCTTTTCTTTAATTTATCAATCTGATCTTACAAGTTGGAAAAAGAAATGAATCATTTTCAGTTTCATATTCATTTAACCACACAAGTTTCTTCCCCTATTATTTTATAAACAGCTTAGAACATGAAAACCTAATATGCATCAAACTACTTTCTTTAAACAGACACCATTGCAAATTCAAGGCCAATTCCAAGACGAGTGTTTAAGAATGTGCAATGAATCAGCAAAGGCTCTGACCGAACTAGCCTTGGCAATCAAGAAAATGACTCCACCAGCCTCTGCAAATCTTCACATCGAAAGGGCGAAGGCCACAGCTGAGAAGCTCAGCTCTCTTCTGGCAAACGGCCATTTTGAGGGTTTGGAGATAGTCCCAGCAGCCACATTGGTCTCTTTGCTTATCGATTCCTTTTCTTGCATCGAGAAAGTCGCAGATTCGACTCGAGAATTGGCTTCCCTTGCAAAGTTTAAGACGGCGGAGCCACCGTCTGGTGCAGTGCGGCCGGCGGTCGATCAAGATTTGCTTGATGTATGA

mRNA sequence

ATGCCCAAACGGACGCCATGTCTGAAGCCTCAAGATCAGAGCTTCTCTCTCTCTCTTTCTTTTTCTCTCGCTTCTGCGGTGGTTATGGCGTCTGAGGGGTACGAAGGAGTGTTGGCTTATGTTGGGTGTGGGTGGGTGAAGAGGATATATGGGAATGTAGAAGATGAAGTGGTGGAGTTGGTGAGGAAGACGAAGAAGGTGGGGGAAGATGATCCGAGAAGGATTGTTCACTCTGTGAAAGTTGGTTTGGCCATCACGTTGGCGTCGTTGTTCTATTACTTCAAGCCTCTTTACGATGGATTTGGAGCCTCTGCAATTTGGACTGTTGTTACAGTCGTCGTTGTCTTCGAGTTCTCTGTTGGTGCGACACTTGGAAAGGGTTTGAATAGAGTGATGGCAACTTTACTAGGAGGGGCCCTAGGATTTGGAACTCATTATTTGGCAAGCTTATGTGGAGATAAAGGCCAGCCTGTAATGCTTGGGGCCATTGTTTTTATAGTAGCTGCAACAGCAACTTTCATGCGATTCTTCCCAAATATGAAGGCGAGATACGACTATGGGCTTCTAATATTCATCTTGACGTTTTCAATGGTGGCTGTGTCCAGTTACAGAGATGATGAGATTCTAAAACTTGCTTTTGATAGGTTTCTGACCATTGTCATTGGAAGCTTCATCACTGCCTTCATTTGCATTTTTGTGTGGCCAATTTGGATTGGTAATGATCTTCACAACCTGCTTGTCACCAACATTCAAAACCTTGCCACCTTCTTACAAGAGTTTGGAGTTGGATATTTCAGATTATCCCGAGAAGGAGCTGAGTTTAACAAAGCCAGCATGCTAAAATATAGAAGTATTCTAACTTCAAAACAAAGTGAAGAATCCATGGCTAACCTGGCTAGATGGGAGCCTTGGCATGGCAAGTTCAGATGTTGCCATCCCTGGAAACAGTACTTGAAGATCAGTTGCTCGGCTCGGGAATGCGCCTATCGGCTTCAAGTTCTTAATGGCTACCTAAACATTGATGATCAAAGAAGTCAGACACCATTGCAAATTCAAGGCCAATTCCAAGACGAGTGTTTAAGAATGTGCAATGAATCAGCAAAGGCTCTGACCGAACTAGCCTTGGCAATCAAGAAAATGACTCCACCAGCCTCTGCAAATCTTCACATCGAAAGGGCGAAGGCCACAGCTGAGAAGCTCAGCTCTCTTCTGGCAAACGGCCATTTTGAGGGTTTGGAGATAGTCCCAGCAGCCACATTGGTCTCTTTGCTTATCGATTCCTTTTCTTGCATCGAGAAAGTCGCAGATTCGACTCGAGAATTGGCTTCCCTTGCAAAGTTTAAGACGGCGGAGCCACCGTCTGGTGCAGTGCGGCCGGCGGTCGATCAAGATTTGCTTGATGTATGA

Coding sequence (CDS)

ATGCCCAAACGGACGCCATGTCTGAAGCCTCAAGATCAGAGCTTCTCTCTCTCTCTTTCTTTTTCTCTCGCTTCTGCGGTGGTTATGGCGTCTGAGGGGTACGAAGGAGTGTTGGCTTATGTTGGGTGTGGGTGGGTGAAGAGGATATATGGGAATGTAGAAGATGAAGTGGTGGAGTTGGTGAGGAAGACGAAGAAGGTGGGGGAAGATGATCCGAGAAGGATTGTTCACTCTGTGAAAGTTGGTTTGGCCATCACGTTGGCGTCGTTGTTCTATTACTTCAAGCCTCTTTACGATGGATTTGGAGCCTCTGCAATTTGGACTGTTGTTACAGTCGTCGTTGTCTTCGAGTTCTCTGTTGGTGCGACACTTGGAAAGGGTTTGAATAGAGTGATGGCAACTTTACTAGGAGGGGCCCTAGGATTTGGAACTCATTATTTGGCAAGCTTATGTGGAGATAAAGGCCAGCCTGTAATGCTTGGGGCCATTGTTTTTATAGTAGCTGCAACAGCAACTTTCATGCGATTCTTCCCAAATATGAAGGCGAGATACGACTATGGGCTTCTAATATTCATCTTGACGTTTTCAATGGTGGCTGTGTCCAGTTACAGAGATGATGAGATTCTAAAACTTGCTTTTGATAGGTTTCTGACCATTGTCATTGGAAGCTTCATCACTGCCTTCATTTGCATTTTTGTGTGGCCAATTTGGATTGGTAATGATCTTCACAACCTGCTTGTCACCAACATTCAAAACCTTGCCACCTTCTTACAAGAGTTTGGAGTTGGATATTTCAGATTATCCCGAGAAGGAGCTGAGTTTAACAAAGCCAGCATGCTAAAATATAGAAGTATTCTAACTTCAAAACAAAGTGAAGAATCCATGGCTAACCTGGCTAGATGGGAGCCTTGGCATGGCAAGTTCAGATGTTGCCATCCCTGGAAACAGTACTTGAAGATCAGTTGCTCGGCTCGGGAATGCGCCTATCGGCTTCAAGTTCTTAATGGCTACCTAAACATTGATGATCAAAGAAGTCAGACACCATTGCAAATTCAAGGCCAATTCCAAGACGAGTGTTTAAGAATGTGCAATGAATCAGCAAAGGCTCTGACCGAACTAGCCTTGGCAATCAAGAAAATGACTCCACCAGCCTCTGCAAATCTTCACATCGAAAGGGCGAAGGCCACAGCTGAGAAGCTCAGCTCTCTTCTGGCAAACGGCCATTTTGAGGGTTTGGAGATAGTCCCAGCAGCCACATTGGTCTCTTTGCTTATCGATTCCTTTTCTTGCATCGAGAAAGTCGCAGATTCGACTCGAGAATTGGCTTCCCTTGCAAAGTTTAAGACGGCGGAGCCACCGTCTGGTGCAGTGCGGCCGGCGGTCGATCAAGATTTGCTTGATGTATGA

Protein sequence

MPKRTPCLKPQDQSFSLSLSFSLASAVVMASEGYEGVLAYVGCGWVKRIYGNVEDEVVELVRKTKKVGEDDPRRIVHSVKVGLAITLASLFYYFKPLYDGFGASAIWTVVTVVVVFEFSVGATLGKGLNRVMATLLGGALGFGTHYLASLCGDKGQPVMLGAIVFIVAATATFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRDDEILKLAFDRFLTIVIGSFITAFICIFVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRLSREGAEFNKASMLKYRSILTSKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSARECAYRLQVLNGYLNIDDQRSQTPLQIQGQFQDECLRMCNESAKALTELALAIKKMTPPASANLHIERAKATAEKLSSLLANGHFEGLEIVPAATLVSLLIDSFSCIEKVADSTRELASLAKFKTAEPPSGAVRPAVDQDLLDV
Homology
BLAST of Sgr025864.1 vs. NCBI nr
Match: XP_022960604.1 (aluminum-activated malate transporter 2-like [Cucurbita moschata])

HSP 1 Score: 616.7 bits (1589), Expect = 1.7e-172
Identity = 321/441 (72.79%), Postives = 368/441 (83.45%), Query Frame = 0

Query: 29  MASEGYEGVLAYVGC-GWVKRIYGNVEDEVVELVRKTKKVGEDDPRRIVHSVKVGLAITL 88
           MA+EGYEGVL Y GC GW+KR+Y  VE EV+E+V+ TK VG  DPRRI+HS+KV LAITL
Sbjct: 1   MAAEGYEGVLVYFGCAGWLKRVYKKVEHEVIEMVKMTKSVGR-DPRRIIHSIKVALAITL 60

Query: 89  ASLFYYFKPLYDGFGASAIWTVVTVVVVFEFSVGATLGKGLNRVMATLLGGALGFGTHYL 148
           AS FYYFKPLYD FGASAIW ++TV+VVFEFSVGAT+GKGLNR++AT+LGGALGFGTHYL
Sbjct: 61  ASFFYYFKPLYDSFGASAIWAIITVIVVFEFSVGATIGKGLNRMIATILGGALGFGTHYL 120

Query: 149 ASLCGDKGQPVMLGAIVFIVAATATFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRDDE 208
           A+LCGDKGQP++LGA+VF+VAA A+FMRFFP+MKARYDYGLLIFILTFSMVAVSSYRDDE
Sbjct: 121 ANLCGDKGQPLILGALVFVVAAIASFMRFFPSMKARYDYGLLIFILTFSMVAVSSYRDDE 180

Query: 209 ILKLAFDRFLTIVIGSFITAFICIFVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRL 268
           +LKLA DRFLTIVIGSFI   ICIFV PIWIGNDLH+ LV N++NLATFLQEFG  YFR 
Sbjct: 181 VLKLASDRFLTIVIGSFIAVSICIFVCPIWIGNDLHDQLVNNVENLATFLQEFGAQYFRS 240

Query: 269 SREGAEFNKASMLKYRSILTSKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSAREC 328
           S+   + NKASM KY++ILTSKQSEESMANLARWEP   KF   HPWKQYL IS SAREC
Sbjct: 241 SQGVEQVNKASMHKYKTILTSKQSEESMANLARWEPGPRKFGFRHPWKQYLNISSSAREC 300

Query: 329 AYRLQVLNGYLNIDDQRSQTPLQIQGQFQDECLRMCNESAKALTELALAIKKMTPPASAN 388
           AYR++VL+ YL  +DQ     +QI+ QF++E LRMC ESAKAL E+A+AIKKM PPA A+
Sbjct: 301 AYRVRVLDVYLENNDQ-----IQIESQFKEEFLRMCKESAKALKEVAMAIKKMAPPALAS 360

Query: 389 LHIERAKATAEKLSSLLANGHFEGLEIVPAATLVSLLIDSFSCIEKVADSTRELASLAKF 448
            HIERAKAT E LSSLLAN  FEGLE++P ATLVSLLID   CIEKVAD TREL  +A  
Sbjct: 361 AHIERAKATTENLSSLLANHRFEGLEMIPTATLVSLLIDLLCCIEKVADCTRELGCVANL 420

Query: 449 KTAEPPSGAVRPAVDQDLLDV 469
           +  E   GAV PA DQDL++V
Sbjct: 421 RRGE-SLGAVVPAADQDLINV 434

BLAST of Sgr025864.1 vs. NCBI nr
Match: KAG7023745.1 (Aluminum-activated malate transporter 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 611.7 bits (1576), Expect = 5.3e-171
Identity = 319/441 (72.34%), Postives = 365/441 (82.77%), Query Frame = 0

Query: 29  MASEGYEGVLAYVGC-GWVKRIYGNVEDEVVELVRKTKKVGEDDPRRIVHSVKVGLAITL 88
           M +EGYEGVL Y GC GW+KR+Y  VE EV+E+V+ TK VG  DPRRI+HS+KV  AITL
Sbjct: 1   MVAEGYEGVLVYFGCAGWLKRVYKKVEHEVIEMVKMTKSVGR-DPRRIIHSIKVASAITL 60

Query: 89  ASLFYYFKPLYDGFGASAIWTVVTVVVVFEFSVGATLGKGLNRVMATLLGGALGFGTHYL 148
           AS FYYFKPLYD FGASAIW ++TV+VVFEFSVGAT+GKGLNR++AT+LGGALGFGTHYL
Sbjct: 61  ASFFYYFKPLYDSFGASAIWAIITVIVVFEFSVGATIGKGLNRMIATILGGALGFGTHYL 120

Query: 149 ASLCGDKGQPVMLGAIVFIVAATATFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRDDE 208
           A+LCGDKG P++LGA+VF+VAA ATFMRFFP+MKARYDYGLLIFILTFSMVAVSSYRDDE
Sbjct: 121 ANLCGDKGLPLILGALVFVVAAIATFMRFFPSMKARYDYGLLIFILTFSMVAVSSYRDDE 180

Query: 209 ILKLAFDRFLTIVIGSFITAFICIFVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRL 268
           +LKLA DRFLTIVIGSFI   ICIFV PIWIGNDLH+ LV N++NLATFLQEFG  YFR 
Sbjct: 181 VLKLASDRFLTIVIGSFIAVSICIFVCPIWIGNDLHDQLVNNVENLATFLQEFGAQYFRS 240

Query: 269 SREGAEFNKASMLKYRSILTSKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSAREC 328
           S+   + NKASM KY++ILTSKQSEESMANLARWEP   KF   HPWKQYL IS SAREC
Sbjct: 241 SQGVEQVNKASMHKYKTILTSKQSEESMANLARWEPGPRKFGFRHPWKQYLNISSSAREC 300

Query: 329 AYRLQVLNGYLNIDDQRSQTPLQIQGQFQDECLRMCNESAKALTELALAIKKMTPPASAN 388
           AYR++VL+ YL  +DQ     +QI+ QF++E LRMC ESAKAL E+A+AIKKM PPA A+
Sbjct: 301 AYRVRVLDVYLKNNDQ-----IQIESQFKEEFLRMCKESAKALKEVAMAIKKMAPPALAS 360

Query: 389 LHIERAKATAEKLSSLLANGHFEGLEIVPAATLVSLLIDSFSCIEKVADSTRELASLAKF 448
            HIERAKAT E LSSLLAN  FEGLE++P ATLVSLLID   CIEKVAD TREL  +A  
Sbjct: 361 AHIERAKATTENLSSLLANHRFEGLEMIPTATLVSLLIDLLCCIEKVADCTRELGCVANL 420

Query: 449 KTAEPPSGAVRPAVDQDLLDV 469
           +  E   GAV PA DQDL++V
Sbjct: 421 RRGE-SLGAVVPAADQDLINV 434

BLAST of Sgr025864.1 vs. NCBI nr
Match: XP_023516721.1 (aluminum-activated malate transporter 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 610.9 bits (1574), Expect = 9.1e-171
Identity = 319/441 (72.34%), Postives = 364/441 (82.54%), Query Frame = 0

Query: 29  MASEGYEGVLAYVGC-GWVKRIYGNVEDEVVELVRKTKKVGEDDPRRIVHSVKVGLAITL 88
           MA+EGYEGVL Y GC GW+KR+Y  VE E++E V+ TK VG D  RRI+HS+KV LAITL
Sbjct: 1   MAAEGYEGVLVYFGCAGWLKRVYNKVEHEMIETVKMTKSVGRDS-RRIIHSIKVALAITL 60

Query: 89  ASLFYYFKPLYDGFGASAIWTVVTVVVVFEFSVGATLGKGLNRVMATLLGGALGFGTHYL 148
           AS FYYFKPLYD FGASAIW ++TV+VVFEFSVGAT+GKGLNR++AT+LGGALGFGTHYL
Sbjct: 61  ASFFYYFKPLYDSFGASAIWAIITVIVVFEFSVGATIGKGLNRMIATILGGALGFGTHYL 120

Query: 149 ASLCGDKGQPVMLGAIVFIVAATATFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRDDE 208
           A+LCGDKGQP++LGA+VF+VAA ATFMRFFP+MKARYDYGLLIFILTFSMVAVSSYRDDE
Sbjct: 121 ANLCGDKGQPLILGALVFVVAAIATFMRFFPSMKARYDYGLLIFILTFSMVAVSSYRDDE 180

Query: 209 ILKLAFDRFLTIVIGSFITAFICIFVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRL 268
           +LKLA DRFLTIVIGSFI   ICIFV PIWIGNDLH+ LV N++NLATFLQEFG  YFR 
Sbjct: 181 VLKLASDRFLTIVIGSFIAVSICIFVCPIWIGNDLHDQLVNNVENLATFLQEFGAQYFRS 240

Query: 269 SREGAEFNKASMLKYRSILTSKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSAREC 328
           S+   + NKASM KY++ILTSKQSEESMANLARWEP   KF   HPWKQYL IS S REC
Sbjct: 241 SQGVEQVNKASMHKYKTILTSKQSEESMANLARWEPGPRKFGFRHPWKQYLNISSSTREC 300

Query: 329 AYRLQVLNGYLNIDDQRSQTPLQIQGQFQDECLRMCNESAKALTELALAIKKMTPPASAN 388
           AYR++VL+ YL  +DQ     +QI+ QF++E LRMCNESAKAL E+A+AIKKM PPA A+
Sbjct: 301 AYRVRVLDVYLENNDQ-----IQIESQFKEEFLRMCNESAKALKEVAMAIKKMAPPALAS 360

Query: 389 LHIERAKATAEKLSSLLANGHFEGLEIVPAATLVSLLIDSFSCIEKVADSTRELASLAKF 448
            HIERAKAT E LSSLLAN  F GLE++P ATLVSLLID   CIEKVAD TREL  +A  
Sbjct: 361 AHIERAKATTEHLSSLLANHRFVGLEMIPTATLVSLLIDLLCCIEKVADCTRELGCVANL 420

Query: 449 KTAEPPSGAVRPAVDQDLLDV 469
           +  E   GAV PA DQDL+ V
Sbjct: 421 RRGE-SLGAVVPAADQDLIHV 434

BLAST of Sgr025864.1 vs. NCBI nr
Match: KAG6590077.1 (Aluminum-activated malate transporter 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 610.5 bits (1573), Expect = 1.2e-170
Identity = 318/441 (72.11%), Postives = 365/441 (82.77%), Query Frame = 0

Query: 29  MASEGYEGVLAYVGC-GWVKRIYGNVEDEVVELVRKTKKVGEDDPRRIVHSVKVGLAITL 88
           M +EGYEGVL Y GC GW+KR+Y  VE E++E+V+ TK VG  DPRRI+HS+KV  AITL
Sbjct: 1   MVAEGYEGVLVYFGCAGWLKRVYKKVEHEMIEMVKMTKSVGR-DPRRIIHSIKVASAITL 60

Query: 89  ASLFYYFKPLYDGFGASAIWTVVTVVVVFEFSVGATLGKGLNRVMATLLGGALGFGTHYL 148
           AS FYYFKPLYD FGASAIW ++TV+VVFEFSVGAT+GKGLNR++AT+LGGALGFGTHYL
Sbjct: 61  ASFFYYFKPLYDSFGASAIWAIITVIVVFEFSVGATIGKGLNRMIATILGGALGFGTHYL 120

Query: 149 ASLCGDKGQPVMLGAIVFIVAATATFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRDDE 208
           A+LCGDKG P++LGA+VF+VAA ATFMRFFP+MKARYDYGLLIFILTFSMVAVSSYRDDE
Sbjct: 121 ANLCGDKGLPLILGALVFVVAAIATFMRFFPSMKARYDYGLLIFILTFSMVAVSSYRDDE 180

Query: 209 ILKLAFDRFLTIVIGSFITAFICIFVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRL 268
           +LKLA DRFLTIVIGSFI   ICIFV PIWIGNDLH+ LV N++NLATFLQEFG  YFR 
Sbjct: 181 VLKLASDRFLTIVIGSFIAVSICIFVCPIWIGNDLHDQLVNNVENLATFLQEFGAQYFRS 240

Query: 269 SREGAEFNKASMLKYRSILTSKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSAREC 328
           S+   + NKASM KY++ILTSKQSEESMANLARWEP   KF   HPWKQYL IS SAREC
Sbjct: 241 SQGVEQVNKASMHKYKTILTSKQSEESMANLARWEPGPRKFGFRHPWKQYLNISSSAREC 300

Query: 329 AYRLQVLNGYLNIDDQRSQTPLQIQGQFQDECLRMCNESAKALTELALAIKKMTPPASAN 388
           AYR++VL+ YL  +DQ     +QI+ QF++E LRMC ESAKAL E+A+AIKKM PPA A+
Sbjct: 301 AYRVRVLDVYLKNNDQ-----IQIESQFKEEFLRMCKESAKALKEVAMAIKKMAPPALAS 360

Query: 389 LHIERAKATAEKLSSLLANGHFEGLEIVPAATLVSLLIDSFSCIEKVADSTRELASLAKF 448
            HIERAKAT E LSSLLAN  FEGLE++P ATLVSLLID   CIEKVAD TREL  +A  
Sbjct: 361 AHIERAKATTENLSSLLANHRFEGLEMIPTATLVSLLIDLLCCIEKVADCTRELGCVANL 420

Query: 449 KTAEPPSGAVRPAVDQDLLDV 469
           +  E   GAV PA DQDL++V
Sbjct: 421 RRGE-SLGAVVPAADQDLINV 434

BLAST of Sgr025864.1 vs. NCBI nr
Match: XP_022988102.1 (aluminum-activated malate transporter 2-like [Cucurbita maxima])

HSP 1 Score: 497.7 bits (1280), Expect = 1.1e-136
Identity = 264/446 (59.19%), Postives = 320/446 (71.75%), Query Frame = 0

Query: 26  AVVMASEGYEGVLAYVGCGWVKRIYGNVEDEVVELVRKTKKVGEDDPRRIVHSVKVGLAI 85
           A   +S  YEG+L +    WVK I+  +    VEL   TKK+ +DD RR+VH++KVGLAI
Sbjct: 2   ATEKSSNEYEGLLPFC-LRWVKPIFTKLPAMAVELATMTKKLAKDDTRRVVHALKVGLAI 61

Query: 86  TLASLFYYFKPLYDGFGASAIWTVVTVVVVFEFSVGATLGKGLNRVMATLLGGALGFGTH 145
           TL SLFYYFKPLYDGFG S IW VVTVVVVFEFSVG TLG+GLNRVMATLL GALGFG H
Sbjct: 62  TLVSLFYYFKPLYDGFGTSTIWAVVTVVVVFEFSVGGTLGRGLNRVMATLLAGALGFGAH 121

Query: 146 YLASLCGDKGQPVMLGAIVFIVAATATFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRD 205
           YLASL GD G+P+MLG  VF++AA +TF+RFFP MKARYDYGLLIFILTF MV++S YRD
Sbjct: 122 YLASLGGDTGRPIMLGLFVFVLAAISTFIRFFPKMKARYDYGLLIFILTFCMVSLSGYRD 181

Query: 206 DEILKLAFDRFLTIVIGSFITAFICIFVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYF 265
           DEI KLA+ R LTI+IG   T F+C+FV P+W G DLH+L+ +NI +LATF   FG  YF
Sbjct: 182 DEIAKLAYSRMLTILIGCCFTVFVCVFVCPVWAGTDLHHLVASNIDSLATFFHGFGAEYF 241

Query: 266 RLSREGAEFNKASMLKYRSILTSKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSAR 325
            L +E  E  K  M KYR++L SK +EES+ NLARWEP HGKFR  HPWK YL+I    R
Sbjct: 242 GLLQEQGEVGKVDMQKYRTVLNSKSNEESLTNLARWEPRHGKFRYRHPWKVYLEIGSLNR 301

Query: 326 ECAYRLQVLNGYLNIDDQRSQTP-LQIQGQFQDECLRMCNESAKALTELALAIKKMTPPA 385
           ECA RL+VLNGYL    +R+Q P  QI G FQ+EC+++C+E +  L ELA+ ++KM PP 
Sbjct: 302 ECACRLEVLNGYL--QTKRTQMPSQQIHGPFQEECMKICSELSNGLRELAMGLRKMVPPL 361

Query: 386 SANLHIERAKATAEKLSSLLANGHFE-------GLEIVPAATLVSLLIDSFSCIEKVADS 445
           +A  HIE+AK  AE L S L    FE        LEIV  A+L  LL+D+  CI K+ADS
Sbjct: 362 TAKAHIEKAKIAAENLKSHLEEWRFEEMNNNKNALEIVHVASLSPLLLDTICCIVKIADS 421

Query: 446 TRELASLAKFKTAEPPSGAVRPAVDQ 464
            ++LAS+A FK  E PS +V P  DQ
Sbjct: 422 VQKLASIAGFKAVEVPS-SVAPEQDQ 443

BLAST of Sgr025864.1 vs. ExPASy Swiss-Prot
Match: Q9SJE8 (Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2 PE=2 SV=2)

HSP 1 Score: 396.0 bits (1016), Expect = 6.0e-109
Identity = 199/416 (47.84%), Postives = 292/416 (70.19%), Query Frame = 0

Query: 55  DEVVELVRKTKKVGEDDPRRIVHSVKVGLAITLASLFYYFKPLYDGFGASAIWTVVTVVV 114
           ++V E+VR+ ++VG++DPRR+VH+ KVGLA+ L S FYY++PLYD FG +A+W V+TVVV
Sbjct: 2   EKVREIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVV 61

Query: 115 VFEFSVGATLGKGLNRVMATLLGGALGFGTHYLASLCGDKGQPVMLGAIVFIVAATATFM 174
           VFEFSVGATLGKGLNR +ATL+ G LG G H+LASL G   +P++L   VF++AA +TF+
Sbjct: 62  VFEFSVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFV 121

Query: 175 RFFPNMKARYDYGLLIFILTFSMVAVSSYRDDEILKLAFDRFLTIVIGSFITAFICIFVW 234
           RFFP +KARYDYG+LIFILTF++++VS +R+DEIL LA  R  T+++G      I IFV 
Sbjct: 122 RFFPRVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVC 181

Query: 235 PIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRLSREG----AEFNKASMLKYRSILTSKQ 294
           P+W G DLH+LL +N   L+ FLQEFG  YF  + +G     E  + ++ +Y+S+L SK 
Sbjct: 182 PVWAGQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGDIKEVEKRRRNLERYKSVLNSKS 241

Query: 295 SEESMANLARWEPWHGKFRCCHPWKQYLKISCSARECAYRLQVLNGYLNIDDQRSQTPLQ 354
           +EE++AN A+WEP HG+FR  HPW+QYL +    R+ AYR+  LN  +N D    Q P+ 
Sbjct: 242 NEEALANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSD---MQIPMD 301

Query: 355 IQGQFQDECLRMCNESAKALTELALAIKKMTPPASANLHIERAKATAEKLSSLLANG--- 414
           I+ + ++   RM +ES K++ E+++++K MT  +S ++H+  +++  + LS+LL +G   
Sbjct: 302 IKKKIEEPLRRMSSESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKSGILN 361

Query: 415 HFEGLEIVPAATLVSLLIDSFSCIEKVADSTRELASLAKFKTAEPPSGAVRPAVDQ 464
             E L+++   T VSLLID  +  EK+++S  ELAS AKFK  + PS +   ++ Q
Sbjct: 362 DVEPLQMISLMTTVSLLIDIVNLTEKISESVHELASAAKFKNKKKPSKSNSGSIGQ 414

BLAST of Sgr025864.1 vs. ExPASy Swiss-Prot
Match: Q9XIN1 (Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7 PE=3 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 2.6e-104
Identity = 200/427 (46.84%), Postives = 282/427 (66.04%), Query Frame = 0

Query: 55  DEVVELVRKTKKVGEDDPRRIVHSVKVGLAITLASLFYYFKPLYDGFGASAIWTVVTVVV 114
           ++V E+VR+ ++V ++DPRR+VHS KVGL + L S FYY++PLYD FG +A+W V+TVVV
Sbjct: 2   EKVREIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVV 61

Query: 115 VFEFSVGATLGKGLNRVMATLLGGALGFGTHYLASLCGDKGQPVMLGAIVFIVAATATFM 174
           VFEFSVGATLGKGLNRV ATL  G LG G H+LAS+ G  G+P++L   VF+ AA +TF+
Sbjct: 62  VFEFSVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFV 121

Query: 175 RFFPNMKARYDYGLLIFILTFSMVAVSSYRDDEILKLAFDRFLTIVIGSFITAFICIFVW 234
           RFFP +KARYDY LLIFILTF++++VS +R+++++KL   R  T++IG      I IFV 
Sbjct: 122 RFFPRVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVC 181

Query: 235 PIWIGNDLHNLLVTNIQNLATF--------------------------LQEFGVGYFR-L 294
           P+W G DLH+L+ +N + L+ F                          L +FG  Y   +
Sbjct: 182 PVWAGQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVV 241

Query: 295 SREGA---EFNKASMLKYRSILTSKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSA 354
             +GA   +  K     Y+S+L SK +EES+AN A+WEP HG+FR  HPWKQYL +    
Sbjct: 242 ENDGAKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELI 301

Query: 355 RECAYRLQVLNGYLNIDDQRSQTPLQIQGQFQDECLRMCNESAKALTELALAIKKMTPPA 414
           R+CAYR+  LN YLN D+Q S   + I+ +  +   RM  ES KA+ E+++++KKMT P+
Sbjct: 302 RQCAYRIHALNSYLNADNQVS---VDIKKKLGEPLRRMSLESGKAMKEMSISLKKMTKPS 361

Query: 415 SANLHIERAKATAEKLSSLLANG---HFEGLEIVPAATLVSLLIDSFSCIEKVADSTREL 449
           S++LH++ AK+  + L++LL +G     E LE+V   T +SLLID  +  EK+ +S  EL
Sbjct: 362 SSDLHVQNAKSACKSLTNLLNSGILKEVEPLELVSLLTAISLLIDIINLTEKILESLHEL 421

BLAST of Sgr025864.1 vs. ExPASy Swiss-Prot
Match: Q9SJE9 (Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 3.9e-100
Identity = 191/410 (46.59%), Postives = 277/410 (67.56%), Query Frame = 0

Query: 55  DEVVELVRKTKKVGEDDPRRIVHSVKVGLAITLASLFYY---FKPLYDGFGASAIWTVVT 114
           ++V E+VR+  +VG +DPRRI+H+ KVGLA+ L S FYY   F P  D FG +A+W V+T
Sbjct: 2   EKVREIVREGIRVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMT 61

Query: 115 VVVVFEFSVGATLGKGLNRVMATLLGGALGFGTHYLASLCGDKGQPVMLGAIVFIVAATA 174
           VVVVFEFSVGATLGKGLNR +ATL+ G LG G H LA L G   +P++L  +VF+ AA +
Sbjct: 62  VVVVFEFSVGATLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALS 121

Query: 175 TFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRDDEILKLAFDRFLTIVIGSFITAFICI 234
           TF+RFFP +K ++DYG+LIFILTF+++++S +RD+EI+ LA  R  T+VIG      I I
Sbjct: 122 TFVRFFPWVKTKFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISI 181

Query: 235 FVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRLSREG----AEFNKASMLKYRSILT 294
           FV P+W G DLH+LL +N   L+ FLQ+FG  YF    +G     E  K ++ +Y+S+L 
Sbjct: 182 FVCPVWAGQDLHSLLASNFDTLSHFLQDFGDEYFEAREKGDYKVVEKRKKNLERYKSVLD 241

Query: 295 SKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSARECAYRLQVLNGYLNIDDQRSQT 354
           SK  EE++AN A WEP HG+FR  HPWKQY+ +    R+CAYR+  LN Y+N D    Q 
Sbjct: 242 SKSDEEALANYAEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSD---FQI 301

Query: 355 PLQIQGQFQDECLRMCNESAKALTELALAIKKMTPPASANLHIERAKATAEKLSSLLANG 414
           P+ I+ + +    RM +ES  ++ E+++++K+M   +S+++H+  ++A  + LS+LL +G
Sbjct: 302 PVDIKKKLETPLRRMSSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKSG 361

Query: 415 ---HFEGLEIVPAATLVSLLIDSFSCIEKVADSTRELASLAKFKTAEPPS 455
                E L+++   T VS+LID  +  EK+++S  ELAS A+FK    P+
Sbjct: 362 ILNDVEPLQMISLMTTVSMLIDIVNLTEKISESVHELASAARFKNKMRPT 408

BLAST of Sgr025864.1 vs. ExPASy Swiss-Prot
Match: Q9SRM9 (Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8 PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.1e-99
Identity = 189/401 (47.13%), Postives = 266/401 (66.33%), Query Frame = 0

Query: 53  VEDEVVELVRKTKKVGEDDPRRIVHSVKVGLAITLASLFYYFKPLYDGFGASAIWTVVTV 112
           ++D+V + V+  +K  +DDPRRI+HS+KVG+A+TL SL YY +PLY  FG + +W ++TV
Sbjct: 23  LKDDVTKRVKNVQKFAKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAILTV 82

Query: 113 VVVFEFSVGATLGKGLNRVMATLLGGALGFGTHYLASLCGDKGQPVMLGAIVFIVAATAT 172
           VVVFEF+VG TL KGLNR  ATL+ GALG G  +LA   G +G+P++LG +VF + A AT
Sbjct: 83  VVVFEFTVGGTLSKGLNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAAAT 142

Query: 173 FMRFFPNMKARYDYGLLIFILTFSMVAVSSYRDDEILKLAFDRFLTIVIGSFITAFICIF 232
           F RFFP +K RYDYG LIFILTFS VA+S YR DEIL +A+ R  TI+IG  I   + IF
Sbjct: 143 FSRFFPRIKQRYDYGALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVSIF 202

Query: 233 VWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRLSREGAEFNKASMLKYRSILTSKQSE 292
           + P+W G DLH ++  NI  LA +L+ F   YF+  +   E   + + +Y+SILTSK +E
Sbjct: 203 ICPVWAGEDLHKMIANNINKLAKYLEGFEGEYFQPEKISKE-TSSCVREYKSILTSKSTE 262

Query: 293 ESMANLARWEPWHGKFRCCHPWKQYLKISCSARECAYRLQVLNGYLNIDDQRSQTPLQIQ 352
           +S+ANLARWEP HG+FR  HPWK+YLKI+   R+CA  L++LNGY+  +D   + P + +
Sbjct: 263 DSLANLARWEPGHGRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSND---KAPQEFE 322

Query: 353 GQFQDECLRMCNESAKALTELALAIKKM-TPPASANLHIERAKATAEKLSSLLANGHFEG 412
            + Q+    M  E  +AL  +A +IK M    A  N HI+ +K   + L   L + + E 
Sbjct: 323 SKIQEPITTMSREVGEALKAIAKSIKTMRNDSACVNAHIDNSKKAIKNLKIALKSSYPET 382

Query: 413 ----LEIVPAATLVSLLIDSFSCIEKVADSTRELASLAKFK 449
               LEI+P  T+ S+LI+  +C+EK+ ++  E + LA FK
Sbjct: 383 YKDLLEIIPGVTMASILIEVVNCVEKIYEAVEEFSGLAHFK 419

BLAST of Sgr025864.1 vs. ExPASy Swiss-Prot
Match: Q76LB1 (Aluminum-activated malate transporter 1 OS=Triticum aestivum OX=4565 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 5.1e-84
Identity = 175/419 (41.77%), Postives = 251/419 (59.90%), Query Frame = 0

Query: 62  RKTKKVGEDDPRRIVHSVKVGLAITLASLFYYFKPLYDGFGASAIWTVVTVVVVFEFSVG 121
           RK      +DPRR+ HS+KVGLA+ L S+ Y+  PL++G G SAIW V+TVVVV E++VG
Sbjct: 40  RKVGGAAREDPRRVAHSLKVGLALALVSVVYFVTPLFNGLGVSAIWAVLTVVVVMEYTVG 99

Query: 122 ATLGKGLNRVMATLLGGALGFGTHYLASL---CGDKGQPVMLGAIVFIVAATATFMRFFP 181
           ATL KGLNR +ATL+ G +  G H LA L   CGD+G+P++L  +VF VA+ ATF+RF P
Sbjct: 100 ATLSKGLNRALATLVAGCIAVGAHQLAELAERCGDQGEPIVLTVLVFFVASAATFLRFIP 159

Query: 182 NMKARYDYGLLIFILTFSMVAVSSYRDDEILKLAFDRFLTIVIGSFITAFICIFVWPIWI 241
            +KA+YDYG+ IFILTF +VAVSSYR +E+++LA  RF TI +G FI     +F++P+W 
Sbjct: 160 EIKAKYDYGVTIFILTFGLVAVSSYRVEELIQLAHQRFYTIAVGVFICLCTTVFLFPVWA 219

Query: 242 GNDLHNLLVTNIQNLATFLQEFGVGYFRLSREGAEF-NKASMLKYRSILTSKQSEESMAN 301
           G D+H L   N+  LA F++      F  +     F  K S   ++S+L SK +E+S+  
Sbjct: 220 GEDVHKLASGNLDKLAQFIEGMEFNCFGENSVANNFGGKDSPQMHKSVLNSKATEDSLCT 279

Query: 302 LARWEPWHGKFRCCHPWKQYLKISCSARECAYRLQVLNGYLNIDDQRSQTPLQIQGQFQD 361
            A+WEP HG+FR  HPW QY K+    R+CA  ++ L  Y+ I   ++Q P     +   
Sbjct: 280 FAKWEPRHGQFRFRHPWSQYQKLGTLCRQCASSMEALASYV-ITTSKTQCPAAANPELSC 339

Query: 362 ECLRMCNE----SAKALTELALAIKKMTPPASANLHIERAKATAEKLSSLLANGHFEGLE 421
           +  + C E    S+K L +LA+A + MT P+  N+ +  A   AE L S LA  +   L+
Sbjct: 340 KVRKTCGEMSLHSSKVLRDLAMATRTMTVPSPVNITMATAVKAAESLRSELAE-NTALLQ 399

Query: 422 IVPAATLVSLLIDSFSCIEKVADSTRELASLAKFKTAEPPS----GAVRPAVDQDLLDV 469
           ++  A   +LL D    ++++A+    LA LA FK  E         V   +D+ L DV
Sbjct: 400 VMHVAVTATLLADLVDRVKEIAECVDVLARLAHFKNPEDTKNVVVSTVSRGIDEPLPDV 456

BLAST of Sgr025864.1 vs. ExPASy TrEMBL
Match: A0A6J1HBH2 (aluminum-activated malate transporter 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461339 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 8.0e-173
Identity = 321/441 (72.79%), Postives = 368/441 (83.45%), Query Frame = 0

Query: 29  MASEGYEGVLAYVGC-GWVKRIYGNVEDEVVELVRKTKKVGEDDPRRIVHSVKVGLAITL 88
           MA+EGYEGVL Y GC GW+KR+Y  VE EV+E+V+ TK VG  DPRRI+HS+KV LAITL
Sbjct: 1   MAAEGYEGVLVYFGCAGWLKRVYKKVEHEVIEMVKMTKSVGR-DPRRIIHSIKVALAITL 60

Query: 89  ASLFYYFKPLYDGFGASAIWTVVTVVVVFEFSVGATLGKGLNRVMATLLGGALGFGTHYL 148
           AS FYYFKPLYD FGASAIW ++TV+VVFEFSVGAT+GKGLNR++AT+LGGALGFGTHYL
Sbjct: 61  ASFFYYFKPLYDSFGASAIWAIITVIVVFEFSVGATIGKGLNRMIATILGGALGFGTHYL 120

Query: 149 ASLCGDKGQPVMLGAIVFIVAATATFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRDDE 208
           A+LCGDKGQP++LGA+VF+VAA A+FMRFFP+MKARYDYGLLIFILTFSMVAVSSYRDDE
Sbjct: 121 ANLCGDKGQPLILGALVFVVAAIASFMRFFPSMKARYDYGLLIFILTFSMVAVSSYRDDE 180

Query: 209 ILKLAFDRFLTIVIGSFITAFICIFVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRL 268
           +LKLA DRFLTIVIGSFI   ICIFV PIWIGNDLH+ LV N++NLATFLQEFG  YFR 
Sbjct: 181 VLKLASDRFLTIVIGSFIAVSICIFVCPIWIGNDLHDQLVNNVENLATFLQEFGAQYFRS 240

Query: 269 SREGAEFNKASMLKYRSILTSKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSAREC 328
           S+   + NKASM KY++ILTSKQSEESMANLARWEP   KF   HPWKQYL IS SAREC
Sbjct: 241 SQGVEQVNKASMHKYKTILTSKQSEESMANLARWEPGPRKFGFRHPWKQYLNISSSAREC 300

Query: 329 AYRLQVLNGYLNIDDQRSQTPLQIQGQFQDECLRMCNESAKALTELALAIKKMTPPASAN 388
           AYR++VL+ YL  +DQ     +QI+ QF++E LRMC ESAKAL E+A+AIKKM PPA A+
Sbjct: 301 AYRVRVLDVYLENNDQ-----IQIESQFKEEFLRMCKESAKALKEVAMAIKKMAPPALAS 360

Query: 389 LHIERAKATAEKLSSLLANGHFEGLEIVPAATLVSLLIDSFSCIEKVADSTRELASLAKF 448
            HIERAKAT E LSSLLAN  FEGLE++P ATLVSLLID   CIEKVAD TREL  +A  
Sbjct: 361 AHIERAKATTENLSSLLANHRFEGLEMIPTATLVSLLIDLLCCIEKVADCTRELGCVANL 420

Query: 449 KTAEPPSGAVRPAVDQDLLDV 469
           +  E   GAV PA DQDL++V
Sbjct: 421 RRGE-SLGAVVPAADQDLINV 434

BLAST of Sgr025864.1 vs. ExPASy TrEMBL
Match: A0A6J1JC53 (aluminum-activated malate transporter 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485449 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 5.4e-137
Identity = 264/446 (59.19%), Postives = 320/446 (71.75%), Query Frame = 0

Query: 26  AVVMASEGYEGVLAYVGCGWVKRIYGNVEDEVVELVRKTKKVGEDDPRRIVHSVKVGLAI 85
           A   +S  YEG+L +    WVK I+  +    VEL   TKK+ +DD RR+VH++KVGLAI
Sbjct: 2   ATEKSSNEYEGLLPFC-LRWVKPIFTKLPAMAVELATMTKKLAKDDTRRVVHALKVGLAI 61

Query: 86  TLASLFYYFKPLYDGFGASAIWTVVTVVVVFEFSVGATLGKGLNRVMATLLGGALGFGTH 145
           TL SLFYYFKPLYDGFG S IW VVTVVVVFEFSVG TLG+GLNRVMATLL GALGFG H
Sbjct: 62  TLVSLFYYFKPLYDGFGTSTIWAVVTVVVVFEFSVGGTLGRGLNRVMATLLAGALGFGAH 121

Query: 146 YLASLCGDKGQPVMLGAIVFIVAATATFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRD 205
           YLASL GD G+P+MLG  VF++AA +TF+RFFP MKARYDYGLLIFILTF MV++S YRD
Sbjct: 122 YLASLGGDTGRPIMLGLFVFVLAAISTFIRFFPKMKARYDYGLLIFILTFCMVSLSGYRD 181

Query: 206 DEILKLAFDRFLTIVIGSFITAFICIFVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYF 265
           DEI KLA+ R LTI+IG   T F+C+FV P+W G DLH+L+ +NI +LATF   FG  YF
Sbjct: 182 DEIAKLAYSRMLTILIGCCFTVFVCVFVCPVWAGTDLHHLVASNIDSLATFFHGFGAEYF 241

Query: 266 RLSREGAEFNKASMLKYRSILTSKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSAR 325
            L +E  E  K  M KYR++L SK +EES+ NLARWEP HGKFR  HPWK YL+I    R
Sbjct: 242 GLLQEQGEVGKVDMQKYRTVLNSKSNEESLTNLARWEPRHGKFRYRHPWKVYLEIGSLNR 301

Query: 326 ECAYRLQVLNGYLNIDDQRSQTP-LQIQGQFQDECLRMCNESAKALTELALAIKKMTPPA 385
           ECA RL+VLNGYL    +R+Q P  QI G FQ+EC+++C+E +  L ELA+ ++KM PP 
Sbjct: 302 ECACRLEVLNGYL--QTKRTQMPSQQIHGPFQEECMKICSELSNGLRELAMGLRKMVPPL 361

Query: 386 SANLHIERAKATAEKLSSLLANGHFE-------GLEIVPAATLVSLLIDSFSCIEKVADS 445
           +A  HIE+AK  AE L S L    FE        LEIV  A+L  LL+D+  CI K+ADS
Sbjct: 362 TAKAHIEKAKIAAENLKSHLEEWRFEEMNNNKNALEIVHVASLSPLLLDTICCIVKIADS 421

Query: 446 TRELASLAKFKTAEPPSGAVRPAVDQ 464
            ++LAS+A FK  E PS +V P  DQ
Sbjct: 422 VQKLASIAGFKAVEVPS-SVAPEQDQ 443

BLAST of Sgr025864.1 vs. ExPASy TrEMBL
Match: A0A6J1H7Y1 (aluminum-activated malate transporter 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461353 PE=3 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 1.0e-135
Identity = 261/446 (58.52%), Postives = 318/446 (71.30%), Query Frame = 0

Query: 26  AVVMASEGYEGVLAYVGCGWVKRIYGNVEDEVVELVRKTKKVGEDDPRRIVHSVKVGLAI 85
           A   +S  YEG+L +    WVK I+  +    VEL   T K+ +DD RR+VH++KVGLAI
Sbjct: 2   ATEKSSNEYEGLLPFC-LRWVKPIFAKLPAIAVELATMTTKLAKDDTRRVVHALKVGLAI 61

Query: 86  TLASLFYYFKPLYDGFGASAIWTVVTVVVVFEFSVGATLGKGLNRVMATLLGGALGFGTH 145
           TL SLFYYFKPLYDGFG S +W VVTV+VVFEFSVG TLG+GLNRVMATLL GALGFG H
Sbjct: 62  TLVSLFYYFKPLYDGFGTSTMWAVVTVIVVFEFSVGGTLGRGLNRVMATLLAGALGFGAH 121

Query: 146 YLASLCGDKGQPVMLGAIVFIVAATATFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRD 205
           YLASL GD G+P+MLG  VF++AA +TF+RFFP MKARYDYGLLIFILTF MV++S YRD
Sbjct: 122 YLASLGGDTGRPIMLGLFVFVLAAISTFIRFFPKMKARYDYGLLIFILTFCMVSLSGYRD 181

Query: 206 DEILKLAFDRFLTIVIGSFITAFICIFVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYF 265
           DEI KLA+ R LTI+IG   T F+C+FV P+W G DLH+L+  NI++LATF   FG  YF
Sbjct: 182 DEIAKLAYSRMLTILIGCCFTVFVCVFVCPVWAGTDLHHLVANNIESLATFFHGFGAEYF 241

Query: 266 RLSREGAEFNKASMLKYRSILTSKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSAR 325
            L +E  E  K  M KYR+IL SK +EES+ NLARWEP HGKFR  HPWK YL+I    R
Sbjct: 242 GLLQEQGEVGKVDMQKYRTILNSKSNEESLTNLARWEPRHGKFRYRHPWKVYLEIGSLNR 301

Query: 326 ECAYRLQVLNGYLNIDDQRSQTP-LQIQGQFQDECLRMCNESAKALTELALAIKKMTPPA 385
           ECA RL+VLNGYL    +R+Q P  QI G FQ+EC+++C+E +  L ELA+ ++KM PP 
Sbjct: 302 ECACRLEVLNGYL--QTKRTQMPSQQIHGPFQEECMKICSELSSGLRELAMGLRKMVPPL 361

Query: 386 SANLHIERAKATAEKLSSLLANGHFE-------GLEIVPAATLVSLLIDSFSCIEKVADS 445
           +A  HIE+AK  AE L S L    FE        LEIV  A+L  LL+D+  CI K+ DS
Sbjct: 362 TAKAHIEKAKIAAENLKSHLEEWRFEEMNNNKNALEIVHVASLSPLLLDTICCIVKIVDS 421

Query: 446 TRELASLAKFKTAEPPSGAVRPAVDQ 464
            ++LAS+A FK  E PS +V P  DQ
Sbjct: 422 VQKLASIAGFKAVEVPS-SVAPEQDQ 443

BLAST of Sgr025864.1 vs. ExPASy TrEMBL
Match: A0A6J1DGC6 (aluminum-activated malate transporter 2-like OS=Momordica charantia OX=3673 GN=LOC111020801 PE=3 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 5.6e-134
Identity = 260/437 (59.50%), Postives = 320/437 (73.23%), Query Frame = 0

Query: 31  SEGYEGVLAYVGCGWVKRIYGNVEDEVVELVRKTKKVGEDDPRRIVHSVKVGLAITLASL 90
           S G+EG+L      WVK + G +    VEL   TK++  DDPRR+VHS+KVGLAITL SL
Sbjct: 7   SNGFEGLLPCCFL-WVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAITLVSL 66

Query: 91  FYYFKPLYDGFGASAIWTVVTVVVVFEFSVGATLGKGLNRVMATLLGGALGFGTHYLASL 150
           FYYF+PLYDGFGAS +W +++V+VVFEFSVG TLG+GLNRVMATL  GALGFG HYLA+L
Sbjct: 67  FYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAHYLANL 126

Query: 151 CGDKGQPVMLGAIVFIVAATATFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRDDEILK 210
            G+KG+P++LG  VFI AA +TF+RFFP MKARYDYG+LIFILTF MV+VS YRDDEI+K
Sbjct: 127 VGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRDDEIVK 186

Query: 211 LAFDRFLTIVIGSFITAFICIFVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRLSRE 270
           LA DR LTI+IG   T F+CIFV P+W G+DLH+L+ TNI +LATFLQ FG  YF L  +
Sbjct: 187 LARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYFGLLGQ 246

Query: 271 GAEFNKASMLKYRSILTSKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSARECAYR 330
           G + +   +  YR IL SK +EES+ NLARWEP H KFR  HPW+QYL+I    R+CAYR
Sbjct: 247 G-QASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRDCAYR 306

Query: 331 LQVLNGYLNIDDQRSQTPL-QIQGQFQDECLRMCNESAKALTELALAIKKMTPPASANLH 390
           L+VLNGYL  +    Q PL QIQ QFQDECL++C+ES ++L EL+LA+KKM PP +   H
Sbjct: 307 LEVLNGYLKTN--LIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVKPH 366

Query: 391 IERAKATAEKLSSLLANGHFE---GLEIVPAATLVSLLIDSFSCIEKVADSTRELASLAK 450
           +ERAK  A+ L S L    FE    +E+V  A+LVSLLIDS  C+EK+ DS ++LAS   
Sbjct: 367 MERAKVAADNLKSHLEEWRFEDSNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLASAGG 426

Query: 451 FKTAEPPSGAVRPAVDQ 464
           FK  E  S  V    DQ
Sbjct: 427 FKAVEGQSCVVVAPEDQ 439

BLAST of Sgr025864.1 vs. ExPASy TrEMBL
Match: B9HQ62 (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_009G017900 PE=3 SV=2)

HSP 1 Score: 487.6 bits (1254), Expect = 5.6e-134
Identity = 246/433 (56.81%), Postives = 313/433 (72.29%), Query Frame = 0

Query: 42  GCGWVKRIYGNVEDEVVELVRKTKKVGEDDPRRIVHSVKVGLAITLASLFYYFKPLYDGF 101
           GCGW+K +    + ++VE  RK KK+G+DDPRR+ HSVKVGLAITL SLFYYF+PLYDGF
Sbjct: 17  GCGWIKSLPERSKAKIVECARKIKKLGQDDPRRVNHSVKVGLAITLVSLFYYFEPLYDGF 76

Query: 102 GASAIWTVVTVVVVFEFSVGATLGKGLNRVMATLLGGALGFGTHYLASLCGDKGQPVMLG 161
           G SA+W V+TVVVVFEFSVGATLG+GLNR +AT L GALGFG H LA+L G+KG+P++LG
Sbjct: 77  GDSAMWAVMTVVVVFEFSVGATLGRGLNRGLATFLAGALGFGAHRLATLSGEKGEPMLLG 136

Query: 162 AIVFIVAATATFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRDDEILKLAFDRFLTIVI 221
             VF++A T TF+RFFP MKARYDYGLLIFILTF +++VS YRDDE+L +A  R  TI+I
Sbjct: 137 LFVFLLATTVTFVRFFPRMKARYDYGLLIFILTFCLISVSGYRDDEMLDMAHKRVSTILI 196

Query: 222 GSFITAFICIFVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRLSREGAEFNKASMLK 281
           GS    F+CI + P+W G+DLHNL  TNI+ L  FL+ FGV +FR   EG   NKAS+  
Sbjct: 197 GSLTAVFVCICICPVWAGDDLHNLAATNIEKLGIFLEHFGVEFFRKPGEGESINKASLQG 256

Query: 282 YRSILTSKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSARECAYRLQVLNGYLNID 341
           Y+S+L SK  EES+ N ARWEP HG+F+  HPWK YLK     R+CAYR++ LNGYLN D
Sbjct: 257 YKSVLNSKNMEESLVNFARWEPGHGQFKFRHPWKHYLKFGSLTRQCAYRVEALNGYLNSD 316

Query: 342 DQRSQTPLQIQGQFQDECLRMCNESAKALTELALAIKKMTPPASANLHIERAKATAEKLS 401
               +TP +IQG  QD C +M +E  KAL ELALAIK+MTPP+SA+ H+ ++K  A+ L 
Sbjct: 317 ---IKTPPEIQGMIQDSCTKMSSELGKALKELALAIKRMTPPSSASSHLVKSKNAAKNLK 376

Query: 402 SLLANGHFEG---LEIVPAATLVSLLIDSFSCIEKVADSTRELASLAKFKTAE------P 461
            LL +    G   LE+VPA T+ SLL +  SC EK+A++  ELASLA+F+  E      P
Sbjct: 377 FLLYSDLCSGINLLEVVPAVTVTSLLFEVISCTEKIAEAIHELASLAQFENVEQEKPKLP 436

Query: 462 PSGAVRPAVDQDL 466
             G ++   + D+
Sbjct: 437 EQGEMQQGANMDV 446

BLAST of Sgr025864.1 vs. TAIR 10
Match: AT1G08440.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 396.0 bits (1016), Expect = 4.3e-110
Identity = 199/416 (47.84%), Postives = 292/416 (70.19%), Query Frame = 0

Query: 55  DEVVELVRKTKKVGEDDPRRIVHSVKVGLAITLASLFYYFKPLYDGFGASAIWTVVTVVV 114
           ++V E+VR+ ++VG++DPRR+VH+ KVGLA+ L S FYY++PLYD FG +A+W V+TVVV
Sbjct: 2   EKVREIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVV 61

Query: 115 VFEFSVGATLGKGLNRVMATLLGGALGFGTHYLASLCGDKGQPVMLGAIVFIVAATATFM 174
           VFEFSVGATLGKGLNR +ATL+ G LG G H+LASL G   +P++L   VF++AA +TF+
Sbjct: 62  VFEFSVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFV 121

Query: 175 RFFPNMKARYDYGLLIFILTFSMVAVSSYRDDEILKLAFDRFLTIVIGSFITAFICIFVW 234
           RFFP +KARYDYG+LIFILTF++++VS +R+DEIL LA  R  T+++G      I IFV 
Sbjct: 122 RFFPRVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVC 181

Query: 235 PIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRLSREG----AEFNKASMLKYRSILTSKQ 294
           P+W G DLH+LL +N   L+ FLQEFG  YF  + +G     E  + ++ +Y+S+L SK 
Sbjct: 182 PVWAGQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGDIKEVEKRRRNLERYKSVLNSKS 241

Query: 295 SEESMANLARWEPWHGKFRCCHPWKQYLKISCSARECAYRLQVLNGYLNIDDQRSQTPLQ 354
           +EE++AN A+WEP HG+FR  HPW+QYL +    R+ AYR+  LN  +N D    Q P+ 
Sbjct: 242 NEEALANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSD---MQIPMD 301

Query: 355 IQGQFQDECLRMCNESAKALTELALAIKKMTPPASANLHIERAKATAEKLSSLLANG--- 414
           I+ + ++   RM +ES K++ E+++++K MT  +S ++H+  +++  + LS+LL +G   
Sbjct: 302 IKKKIEEPLRRMSSESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKSGILN 361

Query: 415 HFEGLEIVPAATLVSLLIDSFSCIEKVADSTRELASLAKFKTAEPPSGAVRPAVDQ 464
             E L+++   T VSLLID  +  EK+++S  ELAS AKFK  + PS +   ++ Q
Sbjct: 362 DVEPLQMISLMTTVSLLIDIVNLTEKISESVHELASAAKFKNKKKPSKSNSGSIGQ 414

BLAST of Sgr025864.1 vs. TAIR 10
Match: AT2G27240.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 380.6 bits (976), Expect = 1.9e-105
Identity = 200/427 (46.84%), Postives = 282/427 (66.04%), Query Frame = 0

Query: 55  DEVVELVRKTKKVGEDDPRRIVHSVKVGLAITLASLFYYFKPLYDGFGASAIWTVVTVVV 114
           ++V E+VR+ ++V ++DPRR+VHS KVGL + L S FYY++PLYD FG +A+W V+TVVV
Sbjct: 2   EKVREIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVV 61

Query: 115 VFEFSVGATLGKGLNRVMATLLGGALGFGTHYLASLCGDKGQPVMLGAIVFIVAATATFM 174
           VFEFSVGATLGKGLNRV ATL  G LG G H+LAS+ G  G+P++L   VF+ AA +TF+
Sbjct: 62  VFEFSVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFV 121

Query: 175 RFFPNMKARYDYGLLIFILTFSMVAVSSYRDDEILKLAFDRFLTIVIGSFITAFICIFVW 234
           RFFP +KARYDY LLIFILTF++++VS +R+++++KL   R  T++IG      I IFV 
Sbjct: 122 RFFPRVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVC 181

Query: 235 PIWIGNDLHNLLVTNIQNLATF--------------------------LQEFGVGYFR-L 294
           P+W G DLH+L+ +N + L+ F                          L +FG  Y   +
Sbjct: 182 PVWAGQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVV 241

Query: 295 SREGA---EFNKASMLKYRSILTSKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSA 354
             +GA   +  K     Y+S+L SK +EES+AN A+WEP HG+FR  HPWKQYL +    
Sbjct: 242 ENDGAKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELI 301

Query: 355 RECAYRLQVLNGYLNIDDQRSQTPLQIQGQFQDECLRMCNESAKALTELALAIKKMTPPA 414
           R+CAYR+  LN YLN D+Q S   + I+ +  +   RM  ES KA+ E+++++KKMT P+
Sbjct: 302 RQCAYRIHALNSYLNADNQVS---VDIKKKLGEPLRRMSLESGKAMKEMSISLKKMTKPS 361

Query: 415 SANLHIERAKATAEKLSSLLANG---HFEGLEIVPAATLVSLLIDSFSCIEKVADSTREL 449
           S++LH++ AK+  + L++LL +G     E LE+V   T +SLLID  +  EK+ +S  EL
Sbjct: 362 SSDLHVQNAKSACKSLTNLLNSGILKEVEPLELVSLLTAISLLIDIINLTEKILESLHEL 421

BLAST of Sgr025864.1 vs. TAIR 10
Match: AT1G08430.1 (aluminum-activated malate transporter 1 )

HSP 1 Score: 366.7 bits (940), Expect = 2.8e-101
Identity = 191/410 (46.59%), Postives = 277/410 (67.56%), Query Frame = 0

Query: 55  DEVVELVRKTKKVGEDDPRRIVHSVKVGLAITLASLFYY---FKPLYDGFGASAIWTVVT 114
           ++V E+VR+  +VG +DPRRI+H+ KVGLA+ L S FYY   F P  D FG +A+W V+T
Sbjct: 2   EKVREIVREGIRVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMT 61

Query: 115 VVVVFEFSVGATLGKGLNRVMATLLGGALGFGTHYLASLCGDKGQPVMLGAIVFIVAATA 174
           VVVVFEFSVGATLGKGLNR +ATL+ G LG G H LA L G   +P++L  +VF+ AA +
Sbjct: 62  VVVVFEFSVGATLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALS 121

Query: 175 TFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRDDEILKLAFDRFLTIVIGSFITAFICI 234
           TF+RFFP +K ++DYG+LIFILTF+++++S +RD+EI+ LA  R  T+VIG      I I
Sbjct: 122 TFVRFFPWVKTKFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISI 181

Query: 235 FVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRLSREG----AEFNKASMLKYRSILT 294
           FV P+W G DLH+LL +N   L+ FLQ+FG  YF    +G     E  K ++ +Y+S+L 
Sbjct: 182 FVCPVWAGQDLHSLLASNFDTLSHFLQDFGDEYFEAREKGDYKVVEKRKKNLERYKSVLD 241

Query: 295 SKQSEESMANLARWEPWHGKFRCCHPWKQYLKISCSARECAYRLQVLNGYLNIDDQRSQT 354
           SK  EE++AN A WEP HG+FR  HPWKQY+ +    R+CAYR+  LN Y+N D    Q 
Sbjct: 242 SKSDEEALANYAEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSD---FQI 301

Query: 355 PLQIQGQFQDECLRMCNESAKALTELALAIKKMTPPASANLHIERAKATAEKLSSLLANG 414
           P+ I+ + +    RM +ES  ++ E+++++K+M   +S+++H+  ++A  + LS+LL +G
Sbjct: 302 PVDIKKKLETPLRRMSSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKSG 361

Query: 415 ---HFEGLEIVPAATLVSLLIDSFSCIEKVADSTRELASLAKFKTAEPPS 455
                E L+++   T VS+LID  +  EK+++S  ELAS A+FK    P+
Sbjct: 362 ILNDVEPLQMISLMTTVSMLIDIVNLTEKISESVHELASAARFKNKMRPT 408

BLAST of Sgr025864.1 vs. TAIR 10
Match: AT3G11680.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 365.2 bits (936), Expect = 8.1e-101
Identity = 189/401 (47.13%), Postives = 266/401 (66.33%), Query Frame = 0

Query: 53  VEDEVVELVRKTKKVGEDDPRRIVHSVKVGLAITLASLFYYFKPLYDGFGASAIWTVVTV 112
           ++D+V + V+  +K  +DDPRRI+HS+KVG+A+TL SL YY +PLY  FG + +W ++TV
Sbjct: 23  LKDDVTKRVKNVQKFAKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAILTV 82

Query: 113 VVVFEFSVGATLGKGLNRVMATLLGGALGFGTHYLASLCGDKGQPVMLGAIVFIVAATAT 172
           VVVFEF+VG TL KGLNR  ATL+ GALG G  +LA   G +G+P++LG +VF + A AT
Sbjct: 83  VVVFEFTVGGTLSKGLNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAAAT 142

Query: 173 FMRFFPNMKARYDYGLLIFILTFSMVAVSSYRDDEILKLAFDRFLTIVIGSFITAFICIF 232
           F RFFP +K RYDYG LIFILTFS VA+S YR DEIL +A+ R  TI+IG  I   + IF
Sbjct: 143 FSRFFPRIKQRYDYGALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVSIF 202

Query: 233 VWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYFRLSREGAEFNKASMLKYRSILTSKQSE 292
           + P+W G DLH ++  NI  LA +L+ F   YF+  +   E   + + +Y+SILTSK +E
Sbjct: 203 ICPVWAGEDLHKMIANNINKLAKYLEGFEGEYFQPEKISKE-TSSCVREYKSILTSKSTE 262

Query: 293 ESMANLARWEPWHGKFRCCHPWKQYLKISCSARECAYRLQVLNGYLNIDDQRSQTPLQIQ 352
           +S+ANLARWEP HG+FR  HPWK+YLKI+   R+CA  L++LNGY+  +D   + P + +
Sbjct: 263 DSLANLARWEPGHGRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSND---KAPQEFE 322

Query: 353 GQFQDECLRMCNESAKALTELALAIKKM-TPPASANLHIERAKATAEKLSSLLANGHFEG 412
            + Q+    M  E  +AL  +A +IK M    A  N HI+ +K   + L   L + + E 
Sbjct: 323 SKIQEPITTMSREVGEALKAIAKSIKTMRNDSACVNAHIDNSKKAIKNLKIALKSSYPET 382

Query: 413 ----LEIVPAATLVSLLIDSFSCIEKVADSTRELASLAKFK 449
               LEI+P  T+ S+LI+  +C+EK+ ++  E + LA FK
Sbjct: 383 YKDLLEIIPGVTMASILIEVVNCVEKIYEAVEEFSGLAHFK 419

BLAST of Sgr025864.1 vs. TAIR 10
Match: AT4G00910.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 289.3 bits (739), Expect = 5.6e-78
Identity = 170/447 (38.03%), Postives = 257/447 (57.49%), Query Frame = 0

Query: 45  WVKRIYGNV-EDEVVELVRKTKKVGEDDPRRIVHSVKVGLAITLASLFYYFKPLYDGFGA 104
           W+K +   V  + V + +RK  ++G DDP ++VH +KVGLA++L S+FYY +PLYDG G 
Sbjct: 36  WLKDLVMKVIMERVAKFMRKAWRIGADDPAKVVHCLKVGLALSLVSIFYYMRPLYDGVGG 95

Query: 105 SAIWTVVTVVVVFEFSVGATLGKGLNRVMATLLGGALGFGTHYLASLCGDKGQPVMLGAI 164
           +A+W ++TVVVVFE +VGAT  K +NRV+AT+L G+LG   H++A+  G K +  ++G  
Sbjct: 96  NAMWAIMTVVVVFESNVGATFCKCVNRVVATILAGSLGIAVHWVATQSG-KAEVFVIGCS 155

Query: 165 VFIVAATATFMRFFPNMKARYDYGLLIFILTFSMVAVSSYRDDEILKLAFDRFLTIVIGS 224
           VF+ A  AT+ RF P+ KAR+DYG +IFILTFS+V+V  YR D++++LA  R  TI IG+
Sbjct: 156 VFLFAFAATYSRFVPSFKARFDYGAMIFILTFSLVSVGGYRVDKLVELAQQRVSTIAIGT 215

Query: 225 FITAFICIFVWPIWIGNDLHNLLVTNIQNLATFLQEFGVGYFR-----LSREGAEFNKAS 284
            I   I +F  PIW G+ LH L+  N++ LA  L      YF+      +R   E     
Sbjct: 216 SICIIITVFFCPIWAGSQLHRLIERNLEKLADSLDGCVAEYFKENEVSTNRNEDENTNMK 275

Query: 285 MLKYRSILTSKQSEESM------------ANLARWEPWHGKFRCCHPWKQYLKISCSARE 344
           +  ++ +L SK +EE+M            ANLARWEP HG F   HPWK Y+KI  + R 
Sbjct: 276 LQGFKCVLNSKGTEEAMPLIRFSGFSFSQANLARWEPAHGSFNFRHPWKLYVKIGAAMRR 335

Query: 345 CAYRLQVLNGYLNIDDQRSQTPLQIQGQFQDECLRMCNESAKALTELALAIKKMTPPASA 404
           CAY L+ L+  +N +   ++ P Q++  F + C+++ + S+K L ELA  +K     +  
Sbjct: 336 CAYCLENLSICINYE---TEAPDQVKNHFGEACMKLSSASSKILRELADMMKNTRKSSKM 395

Query: 405 NLHIERAKATAEKLSSLLANGHFEG-------------------------LEIVPAATLV 449
           +  +    +  ++L   L N   E                           E++P ATLV
Sbjct: 396 DFLVFDMNSAVQELQETLKNVPIETNKPEEVPSEEENKVDSEERTTSMSLHEVLPVATLV 455

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022960604.11.7e-17272.79aluminum-activated malate transporter 2-like [Cucurbita moschata][more]
KAG7023745.15.3e-17172.34Aluminum-activated malate transporter 2, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023516721.19.1e-17172.34aluminum-activated malate transporter 2-like [Cucurbita pepo subsp. pepo][more]
KAG6590077.11.2e-17072.11Aluminum-activated malate transporter 2, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022988102.11.1e-13659.19aluminum-activated malate transporter 2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SJE86.0e-10947.84Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2... [more]
Q9XIN12.6e-10446.84Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7... [more]
Q9SJE93.9e-10046.59Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1... [more]
Q9SRM91.1e-9947.13Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8... [more]
Q76LB15.1e-8441.77Aluminum-activated malate transporter 1 OS=Triticum aestivum OX=4565 GN=ALMT1 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1HBH28.0e-17372.79aluminum-activated malate transporter 2-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JC535.4e-13759.19aluminum-activated malate transporter 2-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1H7Y11.0e-13558.52aluminum-activated malate transporter 2-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1DGC65.6e-13459.50aluminum-activated malate transporter 2-like OS=Momordica charantia OX=3673 GN=L... [more]
B9HQ625.6e-13456.81Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_009G017900 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT1G08440.14.3e-11047.84Aluminium activated malate transporter family protein [more]
AT2G27240.11.9e-10546.84Aluminium activated malate transporter family protein [more]
AT1G08430.12.8e-10146.59aluminum-activated malate transporter 1 [more]
AT3G11680.18.1e-10147.13Aluminium activated malate transporter family protein [more]
AT4G00910.15.6e-7838.03Aluminium activated malate transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020966Aluminum-activated malate transporterPFAMPF11744ALMTcoord: 66..406
e-value: 9.8E-135
score: 450.0
NoneNo IPR availablePANTHERPTHR31086:SF53ALUMINUM ACTIVATED MALATE TRANSPORTER FAMILY PROTEINcoord: 46..453
NoneNo IPR availablePANTHERPTHR31086ALUMINUM-ACTIVATED MALATE TRANSPORTER 10coord: 46..453

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Sgr025864Sgr025864gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sgr025864.1.exon1Sgr025864.1.exon1exon
Sgr025864.1.exon2Sgr025864.1.exon2exon
Sgr025864.1.exon3Sgr025864.1.exon3exon
Sgr025864.1.exon4Sgr025864.1.exon4exon
Sgr025864.1.exon5Sgr025864.1.exon5exon
Sgr025864.1.exon6Sgr025864.1.exon6exon
Sgr025864.1.exon7Sgr025864.1.exon7exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Sgr025864.1cds.Sgr025864.1CDS
cds.Sgr025864.1cds.Sgr025864.1_2CDS
cds.Sgr025864.1cds.Sgr025864.1_3CDS
cds.Sgr025864.1cds.Sgr025864.1_4CDS
cds.Sgr025864.1cds.Sgr025864.1_5CDS
cds.Sgr025864.1cds.Sgr025864.1_6CDS
cds.Sgr025864.1cds.Sgr025864.1_7CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Sgr025864.1Sgr025864.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015743 malate transport
biological_process GO:0034220 ion transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane