Sgr024166.1 (mRNA) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr024166.1
Type	mRNA
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	very-long-chain enoyl-CoA reductase-like
Location	tig00001047: 3770149 .. 3773095 (-)
Sequence length	987
RNA-Seq Expression	Sgr024166.1
Synteny	Sgr024166.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGTCTCGGTAGTTTCTCGCAGTGGAAGAGAGGTTGTTAAGGGAGGCATTCAGCTAAGCGATTCTGTAAGTTTTCAGATTTTTATTAAATTACTGTTTTTTACTCGCTTCCCTTTTCGATTCTTGGAATCACGAAAGGATGAAATCAGTGCAACTTGTTTTGATGGATGTGTTCAGTTAATAGACCTTTCAATTTGGCTTCTTACAAATGAGTAGAAGATTGGACTTTCAGTTTCATGGGAATTGACGTTTTCTTTTTTGCAGGCTTCGGTGGCCGATCTGCAGGAAGCAATTTATAAGCGAAGTGAGTGCAAGATAGTAGAATGTTTCCTTTAATTGTTGCATACATGCTTTATTAGCTTAGCTTGTTGTGATTGTTTAGCTTTGATTCGACATTTTTTAGGAGTGAGTGATAGAACTGGAGTTTGCAGATGTTATGCTACGATATCTACTTGTTGTTTGTCCGATTTCTGGTAGTTGCTAAAAGGATGAATCTAAAAGATTAAATTTAGCCTAGCCTAATAGTTTAAGCTTTTGGGTATGAAATTGCTGAGAAATAAAAAATTTGTTAACTAGTACATTGAGAGTAAAGTACTTCAAAAAGTCCATTGTTTTTTCCTATCTCATGCTTCCAGAAAAATAGTCTTTATTACTTCTAGATGTAACAAAGGATGAGAATTGATCTTTCGCTTGGGTATGCTGATTTTTTCTGCTTTTTTCCGACTATATGTTTATCATTTTTGATTATGTTGCGTAGAGTTAGCATTTCTCTTGTCTTACCGTAGGCACAGAATGAAATGATTTCATCATAGCTGTTTAGTTTTTTTTCCTTTGATGGGTGGGGAGGGGGTGTAAAATAAAATTTTGGGGTTCAATATGATTGTTAATGCACCTCCATAGCTTGAAAATAAATATTTATATCATTGTGGGTGTGGTGTGAAAACTAATGAAAAGTACCTTTCCTCTCAATTTTCTAACAAATGCATACTTGGCATTCTAGATGCTACATAGGCAATCCCTCCCTGTCCCTAGCCAGGTTCTTTCTGTCAGATATTTTAGAGGAAAAACCTCATTGTTAATTTGTCAAAAAATTGAATGATGAGGTGTCTTTTTTAGTTTTTGAAATTACCATGTATATTTTAAACTACAAGGAGTGCATTAGAATAGCTGCTAAATCCTTATGGTTATTCCATGCTTGTATACTTTGTTTTCTTCCTTTTTTTTTTAAATAAATATTTCTTCATTTGCATTATGCCTTCAGGATTTTGGGGTGCATTGTAGCTTGACTGGAGAATTTTCTTATTATAAATGAAATAAAAAAAAAACATGCTTTGCAGGCTAATGCCGTTCCTTTTGCATTATCTACTTGATATTTCCTCTGAACTTCATCATGCTTGCGAAGTAAACTAGGAAACATGGCACTCTTGTTGTTGTCAGCTAAGATAAAACATAAAGTACCTGACCAGAATGTTTAGTTTGGACTTTTGTCATGATATTTTCATATTTGAAGTTCTTTAAGCACACCTAATTAGGATATTATCTATTTCCTTAGTTTTTTCTTCTCCTTTCCCCTGACTTACTAGATTTGGAAGGATGAGGTAACAGAACCTAGTTTTGAACGACAGAGTGATATGATTGAGTGTCTCCAATCTGCTTGACTTATTCAAGGAATAGAATGAGGGAGCGGAGTGGGGGTAGGGTAAGTCTCGATAGTTGGAGTGATTGTTGGTAATGGTGGGGACCACTTTTGGTTATTTTTTCATGAACAGAATAGAAAGCAATGTAAATAGTACACTACAAGGAATATATGTTTGAGATGTATACAAGGGATGATGTGAGCTAACTGTGGATATAGGTCAGAGGGGAAAGAGCAGACCTTTTGAATGGTCTTTACCACATTTTATACCTTCCCACCTTAACTGTTAATAGAATTCCTTAACTCCTCTATGAGTTCTATCAAGATGGAGAAACAAAAAGAAATCTAAAACGACTGGATAGTGTCTGTAGTTCATCTTATAACTGAAACGTTTGATGTTTTTGCCTGATATTGTTTCATGGTTCTTAAGCAGTGGCAAGTTGGCAGTTGTTTAGCATGCATACATGTAAGTCATGTCAGTTTTTATGTAAAGTGCAGCTACCTGATTTCCATGTTTCCATTGAAACAGCCAAAAAGTTCTACCCTGCTAGGCAACGGTTAACTCTTCCCCTTAAACCTGGTTCAAAGGAGAGGCCTACTGTTCTTAACTACAAGAAAAGCCTCAGAGAGTATTGTAGCGATAACTCGGACAACATAACTGTCATCTTCAAGGACTTGGGTCTGCAAGTTTCTTATCGTACGCTCTTTTTTTGGGAATACTTGGGTCCCTTGATCCTCTACCCCATTTTTTATTACCTCCCTGTGTATCAGTTCTTTGGCTACAAGGCAGAACGCGTCATCCATCCAGTTCAGACGTATGCATTGTACTACTGGTGTTTTCACTATTTCAAACGAATCATGGAAACATTTTTCGTGCATAGGTTCAGCCATGCAACTTCACCACTTTCAAACGTATTCCGGAACTGTGCTTACTACTGGACCTTTGGCTCGTATATTGCATATTATGTTAACCACCCACTTTATACCCCTGTTAGTGACCTTCAAATGAAGATTGGCTTTGCATTTGGTGTGGTGTGTCAATTGTCAAACTTCTACTGTCATATCTTGCTGAGAAAATTGCGCAGCCCTGATGGCAATGGGGGATATCAAATCCCAAAGGGGTTTCTGTTCAACATTGTGACATGTGCAAACTACACAACAGAAATATATCAGTGGATAGGTTTCAATATAGCTACACAGACTGTTGCTGGTTGCATCTTTCTTGTGGTTGCTACTCTTATCATGACGAACTGGGCCCTTGCGAAGCATCGTCGCCTCAAGAAGGTATCATCTGACTTATTTCTCTAG

mRNA sequence

ATGAAGGTCTCGGTAGTTTCTCGCAGTGGAAGAGAGGTTGTTAAGGGAGGCATTCAGCTAAGCGATTCTGCTTCGGTGGCCGATCTGCAGGAAGCAATTTATAAGCGAACAGTGGCAAGTTGGCAGTTGTTTAGCATGCATACATGTAAGTCATGTCAGTTTTTATGTAAAGTGCAGCTACCTGATTTCCATGTTTCCATTGAAACAGCCAAAAAGTTCTACCCTGCTAGGCAACGGTTAACTCTTCCCCTTAAACCTGGTTCAAAGGAGAGGCCTACTGTTCTTAACTACAAGAAAAGCCTCAGAGAGTATTGTAGCGATAACTCGGACAACATAACTGTCATCTTCAAGGACTTGGGTCTGCAAGTTTCTTATCGTACGCTCTTTTTTTGGGAATACTTGGGTCCCTTGATCCTCTACCCCATTTTTTATTACCTCCCTGTGTATCAGTTCTTTGGCTACAAGGCAGAACGCGTCATCCATCCAGTTCAGACGTATGCATTGTACTACTGGTGTTTTCACTATTTCAAACGAATCATGGAAACATTTTTCGTGCATAGGTTCAGCCATGCAACTTCACCACTTTCAAACGTATTCCGGAACTGTGCTTACTACTGGACCTTTGGCTCGTATATTGCATATTATGTTAACCACCCACTTTATACCCCTGTTAGTGACCTTCAAATGAAGATTGGCTTTGCATTTGGTGTGGTGTGTCAATTGTCAAACTTCTACTGTCATATCTTGCTGAGAAAATTGCGCAGCCCTGATGGCAATGGGGGATATCAAATCCCAAAGGGGTTTCTGTTCAACATTGTGACATGTGCAAACTACACAACAGAAATATATCAGTGGATAGGTTTCAATATAGCTACACAGACTGTTGCTGGTTGCATCTTTCTTGTGGTTGCTACTCTTATCATGACGAACTGGGCCCTTGCGAAGCATCGTCGCCTCAAGAAGGTATCATCTGACTTATTTCTCTAG

Coding sequence (CDS)

ATGAAGGTCTCGGTAGTTTCTCGCAGTGGAAGAGAGGTTGTTAAGGGAGGCATTCAGCTAAGCGATTCTGCTTCGGTGGCCGATCTGCAGGAAGCAATTTATAAGCGAACAGTGGCAAGTTGGCAGTTGTTTAGCATGCATACATGTAAGTCATGTCAGTTTTTATGTAAAGTGCAGCTACCTGATTTCCATGTTTCCATTGAAACAGCCAAAAAGTTCTACCCTGCTAGGCAACGGTTAACTCTTCCCCTTAAACCTGGTTCAAAGGAGAGGCCTACTGTTCTTAACTACAAGAAAAGCCTCAGAGAGTATTGTAGCGATAACTCGGACAACATAACTGTCATCTTCAAGGACTTGGGTCTGCAAGTTTCTTATCGTACGCTCTTTTTTTGGGAATACTTGGGTCCCTTGATCCTCTACCCCATTTTTTATTACCTCCCTGTGTATCAGTTCTTTGGCTACAAGGCAGAACGCGTCATCCATCCAGTTCAGACGTATGCATTGTACTACTGGTGTTTTCACTATTTCAAACGAATCATGGAAACATTTTTCGTGCATAGGTTCAGCCATGCAACTTCACCACTTTCAAACGTATTCCGGAACTGTGCTTACTACTGGACCTTTGGCTCGTATATTGCATATTATGTTAACCACCCACTTTATACCCCTGTTAGTGACCTTCAAATGAAGATTGGCTTTGCATTTGGTGTGGTGTGTCAATTGTCAAACTTCTACTGTCATATCTTGCTGAGAAAATTGCGCAGCCCTGATGGCAATGGGGGATATCAAATCCCAAAGGGGTTTCTGTTCAACATTGTGACATGTGCAAACTACACAACAGAAATATATCAGTGGATAGGTTTCAATATAGCTACACAGACTGTTGCTGGTTGCATCTTTCTTGTGGTTGCTACTCTTATCATGACGAACTGGGCCCTTGCGAAGCATCGTCGCCTCAAGAAGGTATCATCTGACTTATTTCTCTAG

Protein sequence

MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRTVASWQLFSMHTCKSCQFLCKVQLPDFHVSIETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLGLQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFAFGVVCQLSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTVAGCIFLVVATLIMTNWALAKHRRLKKVSSDLFL

Homology

[Close]

BLAST of Sgr024166.1 vs. NCBI nr
Match: XP_038890322.1 (very-long-chain enoyl-CoA reductase [Benincasa hispida])

HSP 1 Score: 550.8 bits (1418), Expect = 7.8e-153
Identity = 272/322 (84.47%), Postives = 283/322 (87.89%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRTVASWQLFSMHTCKSCQFLCKVQL 60
           MKVSVVSRSGREVVKGGIQLSDSASVADLQ+AIYKRT                       
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIYKRT----------------------- 60

Query: 61  PDFHVSIETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG 120
                     KKFYPARQRLTLP++PGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG
Sbjct: 61  ----------KKFYPARQRLTLPIQPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG 120

Query: 121 LQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIM 180
            QVSYRTLFFWEYLGPLILYPIFYY PVYQFFGYKAERVIHPVQTYA+YYWCFHYFKRIM
Sbjct: 121 PQVSYRTLFFWEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIM 180

Query: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFAFGVVCQ 240
           ETFFVHRFSHATSPLSNVFRNCAYYW+FGSYIAYYVNHPLYTPV DLQMKIGFAFG++CQ
Sbjct: 181 ETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQ 240

Query: 241 LSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTVAGCIF 300
           LSNFYCHILLRKLRSP+GNGGYQIPKGFLFNIVTCANYTTEIYQW+GFNIATQT AG +F
Sbjct: 241 LSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVF 289

Query: 301 LVVATLIMTNWALAKHRRLKKV 323
           LVVA LIMTNWALAKHRRLKK+
Sbjct: 301 LVVAALIMTNWALAKHRRLKKL 289

[Close]

BLAST of Sgr024166.1 vs. NCBI nr
Match: XP_022938888.1 (very-long-chain enoyl-CoA reductase-like [Cucurbita moschata] >KAG6578697.1 Very-long-chain enoyl-CoA reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016235.1 Very-long-chain enoyl-CoA reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 547.4 bits (1409), Expect = 8.7e-152
Identity = 269/322 (83.54%), Postives = 284/322 (88.20%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRTVASWQLFSMHTCKSCQFLCKVQL 60
           MKVSVVSRSGREVVKGGIQLSDSASVADLQ+AIYKRT                       
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIYKRT----------------------- 60

Query: 61  PDFHVSIETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG 120
                     KKFYPARQRLTLP++PGSKERPTVLNYKKSLREYCSDNSDNI+V+FKDLG
Sbjct: 61  ----------KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSDNSDNISVVFKDLG 120

Query: 121 LQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIM 180
            QVSYRTLFFWEYLGPLILYPIFYY PVYQ FGY AER+IHPVQTYA+YYWCFHYFKRIM
Sbjct: 121 PQVSYRTLFFWEYLGPLILYPIFYYFPVYQLFGYNAERIIHPVQTYAMYYWCFHYFKRIM 180

Query: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFAFGVVCQ 240
           ETFFVHRFSHATSPLSNVFRNCAYYW+FGSYIAYYVNHPLYTPVSDLQMKIGFAFG+VCQ
Sbjct: 181 ETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVSDLQMKIGFAFGLVCQ 240

Query: 241 LSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTVAGCIF 300
           +SNFYCHILLRKLRSP+G+GGYQIPKGFLFNIVTCANYTTEIYQW+GFNIATQTVAG +F
Sbjct: 241 VSNFYCHILLRKLRSPEGSGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVF 289

Query: 301 LVVATLIMTNWALAKHRRLKKV 323
           LVVATLIMTNWALAKHRRLKK+
Sbjct: 301 LVVATLIMTNWALAKHRRLKKL 289

[Close]

BLAST of Sgr024166.1 vs. NCBI nr
Match: XP_022134103.1 (very-long-chain enoyl-CoA reductase [Momordica charantia])

HSP 1 Score: 546.6 bits (1407), Expect = 1.5e-151
Identity = 270/322 (83.85%), Postives = 284/322 (88.20%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRTVASWQLFSMHTCKSCQFLCKVQL 60
           MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRT                       
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRT----------------------- 60

Query: 61  PDFHVSIETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG 120
                     KKFYPARQRLTLPL+PGSKERPTVLNYKKSLREYCSDNSDNITV+FKDLG
Sbjct: 61  ----------KKFYPARQRLTLPLQPGSKERPTVLNYKKSLREYCSDNSDNITVVFKDLG 120

Query: 121 LQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIM 180
            QVSYRTLFFWEYLGPLILYPIFYY PVYQFFGYKAERVIHPVQTYA+ YWCFHYFKRI+
Sbjct: 121 TQVSYRTLFFWEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMCYWCFHYFKRIL 180

Query: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFAFGVVCQ 240
           ETFFVHRFSHATSPLSNVFRNCAYYWTFG+YIAYYVNHPLYTPVS+LQMKIGFAFG++CQ
Sbjct: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGAYIAYYVNHPLYTPVSELQMKIGFAFGLLCQ 240

Query: 241 LSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTVAGCIF 300
           +SN YCHILLRKLR+P+G+GGYQIPKGFLFNIVTCANYTTEIYQW+GFNIATQTVAG IF
Sbjct: 241 VSNLYCHILLRKLRNPEGSGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIF 289

Query: 301 LVVATLIMTNWALAKHRRLKKV 323
           LVVATLIMTNWALAKHRRLKK+
Sbjct: 301 LVVATLIMTNWALAKHRRLKKL 289

[Close]

BLAST of Sgr024166.1 vs. NCBI nr
Match: XP_023550073.1 (very-long-chain enoyl-CoA reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 544.7 bits (1402), Expect = 5.6e-151
Identity = 267/322 (82.92%), Postives = 283/322 (87.89%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRTVASWQLFSMHTCKSCQFLCKVQL 60
           MKVSVVSRSGRE+VKGGIQLSDSASVADLQ+AIYKRT                       
Sbjct: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRT----------------------- 60

Query: 61  PDFHVSIETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG 120
                     KKFYPARQRLTLP++PGSKERPTVLNYKKSLREYCSDNSDNI+V+FKDLG
Sbjct: 61  ----------KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSDNSDNISVVFKDLG 120

Query: 121 LQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIM 180
            QV YRTLFFWEYLGPLILYPIFYY PVYQ FGY AER+IHPVQTYA+YYWCFHYFKRIM
Sbjct: 121 PQVLYRTLFFWEYLGPLILYPIFYYFPVYQLFGYNAERIIHPVQTYAMYYWCFHYFKRIM 180

Query: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFAFGVVCQ 240
           ETFFVHRFSHATSPLSNVFRNCAYYW+FGSYIAYYVNHPLYTPVSDLQMKIGFAFG+VCQ
Sbjct: 181 ETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVSDLQMKIGFAFGLVCQ 240

Query: 241 LSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTVAGCIF 300
           +SNFYCHILLRKLRSP+G+GGYQIPKGFLFNIVTCANYTTEIYQW+GFNIATQTVAG +F
Sbjct: 241 VSNFYCHILLRKLRSPEGSGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVF 289

Query: 301 LVVATLIMTNWALAKHRRLKKV 323
           LVVATLIMTNWALAKHRRLKK+
Sbjct: 301 LVVATLIMTNWALAKHRRLKKL 289

[Close]

BLAST of Sgr024166.1 vs. NCBI nr
Match: XP_022992762.1 (very-long-chain enoyl-CoA reductase-like [Cucurbita maxima])

HSP 1 Score: 542.3 bits (1396), Expect = 2.8e-150
Identity = 266/322 (82.61%), Postives = 283/322 (87.89%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRTVASWQLFSMHTCKSCQFLCKVQL 60
           MKVSVVSRSGRE+VKGGIQLSDSASVADLQ+AIYKRT                       
Sbjct: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRT----------------------- 60

Query: 61  PDFHVSIETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG 120
                     KKFYPARQRLTLP++PGSKERPTVLNYKKSLREYCSDNSD I+V+FKDLG
Sbjct: 61  ----------KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSDNSDIISVVFKDLG 120

Query: 121 LQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIM 180
            QVSYRTLFFWEYLGPLILYPIFYY PVYQ FGY AER+IHPVQTYA+YYWCFHYFKRIM
Sbjct: 121 PQVSYRTLFFWEYLGPLILYPIFYYFPVYQLFGYNAERIIHPVQTYAMYYWCFHYFKRIM 180

Query: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFAFGVVCQ 240
           ETFFVHRFSHATSPLSNVFRNCAYYW+FGSYIAYYVNHPLYTPVSDLQMKIGFAFG++CQ
Sbjct: 181 ETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVSDLQMKIGFAFGLLCQ 240

Query: 241 LSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTVAGCIF 300
           +SNFYCHILLRKLRSP+G+GGYQIPKGFLFNIVTCANYTTEIYQW+GFNIATQTVAG +F
Sbjct: 241 VSNFYCHILLRKLRSPEGSGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVF 289

Query: 301 LVVATLIMTNWALAKHRRLKKV 323
           LVVATLIMTNWALAKHRRLKK+
Sbjct: 301 LVVATLIMTNWALAKHRRLKKL 289

[Close]

BLAST of Sgr024166.1 vs. ExPASy Swiss-Prot
Match: Q9M2U2 (Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 4.2e-133
Identity = 226/322 (70.19%), Postives = 262/322 (81.37%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRTVASWQLFSMHTCKSCQFLCKVQL 60
           MKV+VVSRSGREV+K  + L DSA+VADLQEA +KR                        
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKR------------------------ 60

Query: 61  PDFHVSIETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG 120
                    AKKFYP+RQRLTLP+ PGSK++P VLN KKSL+EYC  N++++TV+FKDLG
Sbjct: 61  ---------AKKFYPSRQRLTLPVTPGSKDKPVVLNSKKSLKEYCDGNNNSLTVVFKDLG 120

Query: 121 LQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIM 180
            QVSYRTLFF+EYLGPL++YP+FYY PVY+F GY  + VIHPVQTYA+YYWCFHYFKRI+
Sbjct: 121 AQVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLGYGEDCVIHPVQTYAMYYWCFHYFKRIL 180

Query: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFAFGVVCQ 240
           ETFFVHRFSHATSP+ NVFRNCAYYW+FG+YIAYYVNHPLYTPVSDLQMKIGF FG+VCQ
Sbjct: 181 ETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFGFGLVCQ 240

Query: 241 LSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTVAGCIF 300
           ++NFYCHILL+ LR P G GGYQIP+GFLFNIVTCANYTTEIYQW+GFNIATQT+AG +F
Sbjct: 241 VANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVF 289

Query: 301 LVVATLIMTNWALAKHRRLKKV 323
           L VA LIMTNWAL KH RL+K+
Sbjct: 301 LAVAALIMTNWALGKHSRLRKI 289

[Close]

BLAST of Sgr024166.1 vs. ExPASy Swiss-Prot
Match: Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.7e-47
Identity = 97/215 (45.12%), Postives = 134/215 (62.33%), Query Frame = 0


Query: 113 TVIFKDLGLQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWC 172
           T+ FKDLG Q+S+  +F  EY GPL +YPIFY+L     +G  + +     Q  AL  + 
Sbjct: 78  TLYFKDLGPQISWSLVFICEYAGPLFVYPIFYFLS-NLIYGTDSPKSF--AQKVALVCYS 137

Query: 173 FHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIG 232
            HY KRI ET FVHRFSH T P+ N+F+NC+YYW   + ++Y+VNHPLYT     ++ +G
Sbjct: 138 LHYIKRIYETIFVHRFSHGTMPIFNLFKNCSYYWGCTAMVSYFVNHPLYTEAPIERVYLG 197

Query: 233 FAFGVVCQLSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIAT 292
               ++ ++ N+ CHI LR LR P G+   +IP+G LF  V+C NYT EI  WIGF+I T
Sbjct: 198 LGLWIIGEVFNYICHIQLRNLR-PAGSTERKIPRGLLFEFVSCPNYTVEILSWIGFSILT 257

Query: 293 QTVAGCIFLVVATLIMTNWALAKHRRLKKVSSDLF 328
           QT+   IF ++    M  WA+ KHRR +K   D +
Sbjct: 258 QTLTSWIFALMGAAQMWIWAVGKHRRYRKEFGDKY 288

[Close]

BLAST of Sgr024166.1 vs. ExPASy Swiss-Prot
Match: Q9NZ01 (Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.3e-46
Identity = 118/293 (40.27%), Postives = 157/293 (53.58%), Query Frame = 0


Query: 50  KSCQFLCKVQLPDFHVSI--------ETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSL 109
           K+ + LC +   + H +I        +T  ++YPARQ L L  K  S +   VL      
Sbjct: 12  KTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV- 71

Query: 110 REYCSDNSDNITVIFKDLGLQVSYRTLFFWEYLGPLILYPIFYY-LPVYQFFGYKAERVI 169
                      T+ F+DLG Q+S+ T+F  EY GPL +Y +FY+ +P      Y      
Sbjct: 72  -------GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGHKYDFTSSR 131

Query: 170 HPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPL 229
           H V   A     FHY KR++ET FVHRFSH T PL N+F+NC YYW F +++AYY+NHPL
Sbjct: 132 HTVVHLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPL 191

Query: 230 YTP--VSDLQMKIGFAFGVVCQLSNFYCHILLRKLRSPDGNGGYQIPK------GFLFNI 289
           YTP      Q+K+  A  V+CQL NF  H+ LR LR P G+   +IP        +LF +
Sbjct: 192 YTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLR-PAGSKTRKIPYPTKNPFTWLFLL 251

Query: 290 VTCANYTTEIYQWIGFNIATQTVAGCIFLVVATLIMTNWALAKHRRLKKVSSD 326
           V+C NYT E+  WIGF I TQ +   +F +V    MT WA  KHR   K   D
Sbjct: 252 VSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEFRD 295

[Close]

BLAST of Sgr024166.1 vs. ExPASy Swiss-Prot
Match: Q64232 (Very-long-chain enoyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Tecr PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.3e-46
Identity = 114/267 (42.70%), Postives = 147/267 (55.06%), Query Frame = 0


Query: 68  ETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLGLQVSYRT 127
           +T  ++YPARQ L L  K  S +   VL                 T+ F+DLG Q+S+ T
Sbjct: 38  KTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV--------GTTATLYFRDLGAQISWVT 97

Query: 128 LFFWEYLGPLILYPIFYY-LPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIMETFFVH 187
           +F  EY GPL +Y +FY+ +P      Y      H V   A     FHY KR++ET FVH
Sbjct: 98  VFLTEYAGPLFIYLLFYFRVPFIYGRKYDFTSSRHTVVHLACMCHSFHYIKRLLETLFVH 157

Query: 188 RFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTP--VSDLQMKIGFAFGVVCQLSNF 247
           RFSH T PL N+F+NC YYW F +++AYY+NHPLYTP      Q+K+  A  V+CQL NF
Sbjct: 158 RFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGVQQVKLALAIFVICQLGNF 217

Query: 248 YCHILLRKLRSPDGNGGYQIPK------GFLFNIVTCANYTTEIYQWIGFNIATQTVAGC 307
             H+ LR LR P G+   +IP        +LF +V+C NYT E+  WIGF I TQ V   
Sbjct: 218 SIHMALRDLR-PAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCVPVA 277

Query: 308 IFLVVATLIMTNWALAKHRRLKKVSSD 326
           +F +V    MT WA  KHR   K   D
Sbjct: 278 LFSLVGFTQMTIWAKGKHRSYLKEFRD 295

[Close]

BLAST of Sgr024166.1 vs. ExPASy Swiss-Prot
Match: Q9CY27 (Very-long-chain enoyl-CoA reductase OS=Mus musculus OX=10090 GN=Tecr PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 3.0e-46
Identity = 114/267 (42.70%), Postives = 147/267 (55.06%), Query Frame = 0


Query: 68  ETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLGLQVSYRT 127
           +T  ++YPARQ L L  K  S +   VL                 T+ F+DLG Q+S+ T
Sbjct: 38  KTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV--------GTTATLYFRDLGAQISWVT 97

Query: 128 LFFWEYLGPLILYPIFYY-LPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIMETFFVH 187
           +F  EY GPL +Y +FY+ +P      Y      H V   A     FHY KR++ET FVH
Sbjct: 98  VFLTEYAGPLFIYLLFYFRVPFIYGRKYDFTSSRHTVVHLACMCHSFHYIKRLLETLFVH 157

Query: 188 RFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTP--VSDLQMKIGFAFGVVCQLSNF 247
           RFSH T PL N+F+NC YYW F +++AYY+NHPLYTP      Q+K+  A  V+CQL NF
Sbjct: 158 RFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGVQQVKLALAVFVICQLGNF 217

Query: 248 YCHILLRKLRSPDGNGGYQIPK------GFLFNIVTCANYTTEIYQWIGFNIATQTVAGC 307
             H+ LR LR P G+   +IP        +LF +V+C NYT E+  WIGF I TQ V   
Sbjct: 218 SIHMALRDLR-PAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAILTQCVPVA 277

Query: 308 IFLVVATLIMTNWALAKHRRLKKVSSD 326
           +F +V    MT WA  KHR   K   D
Sbjct: 278 LFSLVGFTQMTIWAKGKHRSYLKEFRD 295

[Close]

BLAST of Sgr024166.1 vs. ExPASy TrEMBL
Match: A0A6J1FK64 (very-long-chain enoyl-CoA reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111444958 PE=3 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 4.2e-152
Identity = 269/322 (83.54%), Postives = 284/322 (88.20%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRTVASWQLFSMHTCKSCQFLCKVQL 60
           MKVSVVSRSGREVVKGGIQLSDSASVADLQ+AIYKRT                       
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIYKRT----------------------- 60

Query: 61  PDFHVSIETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG 120
                     KKFYPARQRLTLP++PGSKERPTVLNYKKSLREYCSDNSDNI+V+FKDLG
Sbjct: 61  ----------KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSDNSDNISVVFKDLG 120

Query: 121 LQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIM 180
            QVSYRTLFFWEYLGPLILYPIFYY PVYQ FGY AER+IHPVQTYA+YYWCFHYFKRIM
Sbjct: 121 PQVSYRTLFFWEYLGPLILYPIFYYFPVYQLFGYNAERIIHPVQTYAMYYWCFHYFKRIM 180

Query: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFAFGVVCQ 240
           ETFFVHRFSHATSPLSNVFRNCAYYW+FGSYIAYYVNHPLYTPVSDLQMKIGFAFG+VCQ
Sbjct: 181 ETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVSDLQMKIGFAFGLVCQ 240

Query: 241 LSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTVAGCIF 300
           +SNFYCHILLRKLRSP+G+GGYQIPKGFLFNIVTCANYTTEIYQW+GFNIATQTVAG +F
Sbjct: 241 VSNFYCHILLRKLRSPEGSGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVF 289

Query: 301 LVVATLIMTNWALAKHRRLKKV 323
           LVVATLIMTNWALAKHRRLKK+
Sbjct: 301 LVVATLIMTNWALAKHRRLKKL 289

[Close]

BLAST of Sgr024166.1 vs. ExPASy TrEMBL
Match: A0A6J1C130 (very-long-chain enoyl-CoA reductase OS=Momordica charantia OX=3673 GN=LOC111006458 PE=3 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 7.2e-152
Identity = 270/322 (83.85%), Postives = 284/322 (88.20%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRTVASWQLFSMHTCKSCQFLCKVQL 60
           MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRT                       
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRT----------------------- 60

Query: 61  PDFHVSIETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG 120
                     KKFYPARQRLTLPL+PGSKERPTVLNYKKSLREYCSDNSDNITV+FKDLG
Sbjct: 61  ----------KKFYPARQRLTLPLQPGSKERPTVLNYKKSLREYCSDNSDNITVVFKDLG 120

Query: 121 LQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIM 180
            QVSYRTLFFWEYLGPLILYPIFYY PVYQFFGYKAERVIHPVQTYA+ YWCFHYFKRI+
Sbjct: 121 TQVSYRTLFFWEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMCYWCFHYFKRIL 180

Query: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFAFGVVCQ 240
           ETFFVHRFSHATSPLSNVFRNCAYYWTFG+YIAYYVNHPLYTPVS+LQMKIGFAFG++CQ
Sbjct: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGAYIAYYVNHPLYTPVSELQMKIGFAFGLLCQ 240

Query: 241 LSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTVAGCIF 300
           +SN YCHILLRKLR+P+G+GGYQIPKGFLFNIVTCANYTTEIYQW+GFNIATQTVAG IF
Sbjct: 241 VSNLYCHILLRKLRNPEGSGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIF 289

Query: 301 LVVATLIMTNWALAKHRRLKKV 323
           LVVATLIMTNWALAKHRRLKK+
Sbjct: 301 LVVATLIMTNWALAKHRRLKKL 289

[Close]

BLAST of Sgr024166.1 vs. ExPASy TrEMBL
Match: A0A6J1JQU0 (very-long-chain enoyl-CoA reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111489004 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.3e-150
Identity = 266/322 (82.61%), Postives = 283/322 (87.89%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRTVASWQLFSMHTCKSCQFLCKVQL 60
           MKVSVVSRSGRE+VKGGIQLSDSASVADLQ+AIYKRT                       
Sbjct: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRT----------------------- 60

Query: 61  PDFHVSIETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG 120
                     KKFYPARQRLTLP++PGSKERPTVLNYKKSLREYCSDNSD I+V+FKDLG
Sbjct: 61  ----------KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSDNSDIISVVFKDLG 120

Query: 121 LQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIM 180
            QVSYRTLFFWEYLGPLILYPIFYY PVYQ FGY AER+IHPVQTYA+YYWCFHYFKRIM
Sbjct: 121 PQVSYRTLFFWEYLGPLILYPIFYYFPVYQLFGYNAERIIHPVQTYAMYYWCFHYFKRIM 180

Query: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFAFGVVCQ 240
           ETFFVHRFSHATSPLSNVFRNCAYYW+FGSYIAYYVNHPLYTPVSDLQMKIGFAFG++CQ
Sbjct: 181 ETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVSDLQMKIGFAFGLLCQ 240

Query: 241 LSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTVAGCIF 300
           +SNFYCHILLRKLRSP+G+GGYQIPKGFLFNIVTCANYTTEIYQW+GFNIATQTVAG +F
Sbjct: 241 VSNFYCHILLRKLRSPEGSGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVF 289

Query: 301 LVVATLIMTNWALAKHRRLKKV 323
           LVVATLIMTNWALAKHRRLKK+
Sbjct: 301 LVVATLIMTNWALAKHRRLKKL 289

[Close]

BLAST of Sgr024166.1 vs. ExPASy TrEMBL
Match: A0A6J1E1Q3 (very-long-chain enoyl-CoA reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111429991 PE=3 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.5e-149
Identity = 266/322 (82.61%), Postives = 282/322 (87.58%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRTVASWQLFSMHTCKSCQFLCKVQL 60
           MKVSVVSRSGREVV GGIQLSDSASVADLQ+AIYKRT                       
Sbjct: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYKRT----------------------- 60

Query: 61  PDFHVSIETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG 120
                     KKFYPARQRLTLP++PGSKERPTVLNYKKSL+EYCS+NSDNITV+FKDLG
Sbjct: 61  ----------KKFYPARQRLTLPVQPGSKERPTVLNYKKSLKEYCSNNSDNITVVFKDLG 120

Query: 121 LQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIM 180
            QVSYRTLFF+EYLGPLILYPIFYY PVYQFFGYKAERVIHPVQTYA+YYWCFHYFKRIM
Sbjct: 121 PQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIM 180

Query: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFAFGVVCQ 240
           ETFFVHRFSHATSPLSNVFRNCAYYW+FGSYIAYYVNHPLY+PV D QMKIGFAFG++CQ
Sbjct: 181 ETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYSPVGDFQMKIGFAFGLLCQ 240

Query: 241 LSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTVAGCIF 300
           + NFYCHILLRKLRSP+GNGGYQIPKGFLFNIVTCANYTTEIYQW+GFNIATQTVAG IF
Sbjct: 241 VCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIF 289

Query: 301 LVVATLIMTNWALAKHRRLKKV 323
           LVVATLIMTNWALAKHRRLKK+
Sbjct: 301 LVVATLIMTNWALAKHRRLKKL 289

[Close]

BLAST of Sgr024166.1 vs. ExPASy TrEMBL
Match: A0A6J1JNI3 (very-long-chain enoyl-CoA reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111487465 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.3e-149
Identity = 266/322 (82.61%), Postives = 281/322 (87.27%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRTVASWQLFSMHTCKSCQFLCKVQL 60
           MKVSVVSRSGREVV GGIQLSDSASVADLQ+ IYKRT                       
Sbjct: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDVIYKRT----------------------- 60

Query: 61  PDFHVSIETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG 120
                     KKFYPARQRLTLP++PG KERPTVLNYKKSLREYCS+NSDNITV+FKDLG
Sbjct: 61  ----------KKFYPARQRLTLPVQPGLKERPTVLNYKKSLREYCSNNSDNITVVFKDLG 120

Query: 121 LQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIM 180
            QVSYRTLFF+EYLGPLILYPIFYY PVYQFFGYKAERVIHPVQTYA+YYWCFHYFKRIM
Sbjct: 121 PQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIM 180

Query: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFAFGVVCQ 240
           ETFFVHRFSHATSPLSNVFRNCAYYW+FGSYIAYYVNHPLY+PV DLQMKIGFAFG++CQ
Sbjct: 181 ETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYSPVGDLQMKIGFAFGLLCQ 240

Query: 241 LSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTVAGCIF 300
           + NFYCHILLRKLRSP+GNGGYQIPKGFLFNIVTCANYTTEIYQW+GFNIATQTVAG IF
Sbjct: 241 VCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIF 289

Query: 301 LVVATLIMTNWALAKHRRLKKV 323
           LVVATLIMTNWALAKHRRLKK+
Sbjct: 301 LVVATLIMTNWALAKHRRLKKL 289

[Close]

BLAST of Sgr024166.1 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 475.7 bits (1223), Expect = 3.0e-134
Identity = 226/322 (70.19%), Postives = 262/322 (81.37%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQEAIYKRTVASWQLFSMHTCKSCQFLCKVQL 60
           MKV+VVSRSGREV+K  + L DSA+VADLQEA +KR                        
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKR------------------------ 60

Query: 61  PDFHVSIETAKKFYPARQRLTLPLKPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLG 120
                    AKKFYP+RQRLTLP+ PGSK++P VLN KKSL+EYC  N++++TV+FKDLG
Sbjct: 61  ---------AKKFYPSRQRLTLPVTPGSKDKPVVLNSKKSLKEYCDGNNNSLTVVFKDLG 120

Query: 121 LQVSYRTLFFWEYLGPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIM 180
            QVSYRTLFF+EYLGPL++YP+FYY PVY+F GY  + VIHPVQTYA+YYWCFHYFKRI+
Sbjct: 121 AQVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLGYGEDCVIHPVQTYAMYYWCFHYFKRIL 180

Query: 181 ETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKIGFAFGVVCQ 240
           ETFFVHRFSHATSP+ NVFRNCAYYW+FG+YIAYYVNHPLYTPVSDLQMKIGF FG+VCQ
Sbjct: 181 ETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFGFGLVCQ 240

Query: 241 LSNFYCHILLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTVAGCIF 300
           ++NFYCHILL+ LR P G GGYQIP+GFLFNIVTCANYTTEIYQW+GFNIATQT+AG +F
Sbjct: 241 VANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVF 289

Query: 301 LVVATLIMTNWALAKHRRLKKV 323
           L VA LIMTNWAL KH RL+K+
Sbjct: 301 LAVAALIMTNWALGKHSRLRKI 289

[Close]

BLAST of Sgr024166.1 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 72.8 bits (177), Expect = 5.8e-13
Identity = 54/189 (28.57%), Postives = 89/189 (47.09%), Query Frame = 0


Query: 135 GPLILYPIFYYLPVYQFFGYKAERVIHPVQTYALYYWCFHYFKRIMETFFVHRFSHATSP 194
           G L+LY   +      FF   ++ +   +   AL     H+FKR+ E  F+H++S   + 
Sbjct: 68  GMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSAL---ALHFFKRVFEVLFIHKYSGGMA- 127

Query: 195 LSNVFRNCAYYWTFGSYIAYYVNHPLYTPVSDLQMKI-GFAFGVVCQLSNFYCHILLRKL 254
           + +     + Y++  + + Y  N  L        MK+ G    VV  + N Y H+LL KL
Sbjct: 128 IDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLLAKL 187

Query: 255 RSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGFNIATQTV-------AGCIFLVVATL 314
           R  DG   Y+IPKG LF+I+ C +Y  EI  +  F + +QT+          ++L+  + 
Sbjct: 188 RKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGRSY 247

Query: 315 IMTNWALAK 316
               W L+K
Sbjct: 248 ATRTWYLSK 252

[Close]

BLAST of Sgr024166.1 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.2e-05
Identity = 28/89 (31.46%), Postives = 46/89 (51.69%), Query Frame = 0


Query: 233 FAFGVVCQLSNFYCHI----LLRKLRSPDGNGGYQIPKGFLFNIVTCANYTTEIYQWIGF 292
           F  G+V  ++  Y +I     L +L+  +  GGY IP+G  F +V+C NY  E  +W+G+
Sbjct: 153 FVIGMVVFITGMYINITSDRTLVRLKK-ENRGGYVIPRGGWFELVSCPNYFGEAIEWLGW 212

Query: 293 NIATQTVAGCIFLVVATLIMTNWALAKHR 318
            + T + AG  F +     +   A A H+
Sbjct: 213 AVMTWSWAGIGFFLYTCSNLFPRARASHK 240

The following BLAST results are available for this feature:

BLAST of Sgr024166.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038890322.1	7.8e-153	84.47	very-long-chain enoyl-CoA reductase [Benincasa hispida]	[more]
XP_022938888.1	8.7e-152	83.54	very-long-chain enoyl-CoA reductase-like [Cucurbita moschata] >KAG6578697.1 Very...	[more]
XP_022134103.1	1.5e-151	83.85	very-long-chain enoyl-CoA reductase [Momordica charantia]	[more]
XP_023550073.1	5.6e-151	82.92	very-long-chain enoyl-CoA reductase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022992762.1	2.8e-150	82.61	very-long-chain enoyl-CoA reductase-like [Cucurbita maxima]	[more]

BLAST of Sgr024166.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2U2	4.2e-133	70.19	Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 ...	[more]
Q55C17	2.7e-47	45.12	Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn...	[more]
Q9NZ01	1.3e-46	40.27	Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1	[more]
Q64232	2.3e-46	42.70	Very-long-chain enoyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Tecr PE=1 S...	[more]
Q9CY27	3.0e-46	42.70	Very-long-chain enoyl-CoA reductase OS=Mus musculus OX=10090 GN=Tecr PE=1 SV=1	[more]

BLAST of Sgr024166.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FK64	4.2e-152	83.54	very-long-chain enoyl-CoA reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1C130	7.2e-152	83.85	very-long-chain enoyl-CoA reductase OS=Momordica charantia OX=3673 GN=LOC1110064...	[more]
A0A6J1JQU0	1.3e-150	82.61	very-long-chain enoyl-CoA reductase-like OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
A0A6J1E1Q3	1.5e-149	82.61	very-long-chain enoyl-CoA reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1JNI3	3.3e-149	82.61	very-long-chain enoyl-CoA reductase-like OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]

BLAST of Sgr024166.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55360.1	3.0e-134	70.19	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT5G16010.1	5.8e-13	28.57	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	1.2e-05	31.46	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 192..321 e-value: 1.8E-26 score: 92.9
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 232..313 score: 21.622391
None	No IPR available	PANTHER	PTHR10556:SF28	SC2	coord: 59..321
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 59..321

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sgr024166	Sgr024166	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sgr024166.1.exon3	Sgr024166.1.exon3	exon
Sgr024166.1.exon2	Sgr024166.1.exon2	exon
Sgr024166.1.exon1	Sgr024166.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Sgr024166.1	cds.Sgr024166.1_3	CDS
cds.Sgr024166.1	cds.Sgr024166.1_2	CDS
cds.Sgr024166.1	cds.Sgr024166.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sgr024166.1	Sgr024166.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0042761	very long-chain fatty acid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors
molecular_function	GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity