Sgr023718.1 (mRNA) Monk fruit (Qingpiguo) v1

BLAST of Sgr023718.1 vs. NCBI nr
Match: XP_022140625.1 (uncharacterized protein LOC111011235 [Momordica charantia])

HSP 1 Score: 216.1 bits (549), Expect = 4.5e-52
Identity = 167/331 (50.45%), Postives = 206/331 (62.24%), Query Frame = 0

Query: 1   MKFFVSIFAVSITVFFFFSLSAAYSWIFYLSATIHKNYMFLFCNSLLVFICLNSRSNNGV 60
           MK F+SIFAVSITVFFF SLSAA+SW  YLSATIHK+YMFL CNSLLVFICLNS S   V
Sbjct: 1   MKIFISIFAVSITVFFFLSLSAAFSWTLYLSATIHKSYMFLLCNSLLVFICLNSPST--V 60

Query: 61  SSEKDYGQEVAEKKQAAGYYESRPLNRCRTVGEVGDEKETGSLHVEHVREDQVLIAEDDD 120
           +S                      L+  RTVGEVGDEKETGSLHV  V EDQ+LIAED+D
Sbjct: 61  NS----------------------LDGSRTVGEVGDEKETGSLHVVRVFEDQILIAEDND 120

Query: 121 EKAGETLYIAVEEIYDDDCDGEVAGDDDEKEIGSLRAVQVREDGVQIEEDDDEKAGETLY 180
           EKAGETL+  VEE  DD+                   ++VRED +            TL+
Sbjct: 121 EKAGETLF--VEETRDDE-------------------LEVREDDM------------TLF 180

Query: 181 VVNEEIYDYDRNGEVAGEDDEKETRSLHVVQVREDWVLIVEDNDEKA----VETLYVVDE 240
           V+ EEI+D         E + K+ R+L VV+       I +DN+++     V+ +  + E
Sbjct: 181 VI-EEIHD--------DELEAKDDRTLSVVEE------IHDDNEQEQGGGDVDMILSIVE 240

Query: 241 EIYDDDRDGEVAEDDKTLPVNVEESQEDELEVVEDDQTLSVIEEICNDD-EQEQGGGIDT 300
           E  DD+   EV EDD TL  +VEE+Q+DELE   DD+TLSV++E  +D+ EQEQGGG D 
Sbjct: 241 ETRDDEL--EVVEDDMTL-FDVEETQDDELEAA-DDKTLSVVKETHDDEQEQEQGGGNDM 255

Query: 301 ISLEELNKKFEEFIRTTKEQIRKADQTIQCH 327
           ISLEELNKKF+EFIRTT+ +IR    TIQC+
Sbjct: 301 ISLEELNKKFDEFIRTTRMKIRAELLTIQCY 255

BLAST of Sgr023718.1 vs. NCBI nr
Match: XP_038902155.1 (uncharacterized protein LOC120088789 [Benincasa hispida])

HSP 1 Score: 193.7 bits (491), Expect = 2.4e-45
Identity = 153/318 (48.11%), Postives = 188/318 (59.12%), Query Frame = 0

Query: 1   MKFFVSIFAVSITVFFFFSLS-AAYSWIFYLSATIHKNYMFLFCNSLLVFICLNSRSNNG 60
           MK F+SIF+VSI++FF FSLS AAYSWIFYLS TIHK+YMFLFCNSLLVFICLNSR +  
Sbjct: 1   MKNFLSIFSVSISLFFLFSLSIAAYSWIFYLSTTIHKDYMFLFCNSLLVFICLNSRLS-- 60

Query: 61  VSSEKDYGQEVAEKKQAAGYYESRPLNRCRTVGEVGDEKETGSLHVEHVREDQVLIAEDD 120
           VS EKD  QE+AEK+QAAG  ESR L+R RTVG+V                         
Sbjct: 61  VSLEKDRRQELAEKRQAAGCCESRLLDRRRTVGDV------------------------- 120

Query: 121 DEKAGETLYIAVEEIYDDDCDGEVAGDDDEKEIGSLRAVQVREDGVQIEEDDDEKAGETL 180
               GETL + VEE Y DD  G++A  DDE        + V+ +    +E D  +   TL
Sbjct: 121 ----GETLSVVVEETYGDDYYGDLAAGDDE-------TLLVKVEVTPTDELDAVEDDRTL 180

Query: 181 YVVNEEIYDYDRNGEVAGEDDEKETRSLHVVQVREDWVLIVEDNDEKAVETLYVVDEEIY 240
            VV EE            + DE ET              +VEDN     +TL V++E   
Sbjct: 181 SVVIEET-----------KGDELET--------------VVEDN-----KTLSVIEE--- 240

Query: 241 DDDRDGEVAEDDKTLPVNVEESQEDELEVVEDDQTLSVIEEICNDDEQEQGGGIDTISLE 300
             D D EV ED KTL + +EE ++ EL +VE D+TLS+ EE  ND+EQEQGG +D+ISLE
Sbjct: 241 TQDEDFEVVEDAKTLRI-IEEFRDHELGIVEGDKTLSIFEETRNDEEQEQGGSVDSISLE 246

Query: 301 ELNKKFEEFIRTTKEQIR 318
           ELN+K EEFIR  K+ IR
Sbjct: 301 ELNRKCEEFIRRMKKDIR 246

BLAST of Sgr023718.1 vs. NCBI nr
Match: XP_023512249.1 (uncharacterized protein LOC111777037 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 83.6 bits (205), Expect = 3.5e-12
Identity = 73/166 (43.98%), Postives = 92/166 (55.42%), Query Frame = 0

Query: 5   VSIFAVSITVFFF-FSLSAAYSWIFYLSATIHKNYMFLFCNSLLVFICLNSRSNNGVSSE 64
           + IFAVSI +FFF FS S AYSWIFY SATIHK+YMF FCN+LL+F+ LNSRS+      
Sbjct: 1   MKIFAVSIPLFFFIFSFSVAYSWIFYFSATIHKDYMFFFCNTLLLFLSLNSRSS------ 60

Query: 65  KDYGQEVAEKKQAAGYYESRPLNRCRTVGEVGDEKETGSLHVEHVREDQVLIAEDDDEKA 124
                 VA+K+QAAG  + R          V  EKE G L V+ V    VL    D E  
Sbjct: 61  ------VADKEQAAG--DGR--------RSVDGEKEMGLLRVQQVSRRLVLQETRDHEL- 120

Query: 125 GETLYIAVEEIYDDDCDGEVAGDDDEKEIGSLRAVQVREDGVQIEE 170
            E    A+EE ++D+ +G     DDE E    +    R D + +EE
Sbjct: 121 -EMCSFALEEAHNDELEGM---KDDECEHEQEQEQGHRVDTIGLEE 139

BLAST of Sgr023718.1 vs. NCBI nr
Match: KGN46604.1 (hypothetical protein Csa_005627 [Cucumis sativus])

HSP 1 Score: 74.7 bits (182), Expect = 1.6e-09
Identity = 55/112 (49.11%), Postives = 73/112 (65.18%), Query Frame = 0

Query: 215 DWVLIVEDNDEKAVE---TLYVVDEEIYDDDRDGEVAEDDKTLPVNVEESQEDELEVVED 274
           D V + + N   A E   TL V+ E   D   + E+ +DD+TL V +EE+Q+ + +VVED
Sbjct: 20  DNVEVTQANQLDAPEEDMTLSVIKETKGD---ELELVKDDRTLSV-IEETQDYDSDVVED 79

Query: 275 ------DQTLSVIEEICNDDEQEQGGGIDTISLEELNKKFEEFIRTTKEQIR 318
                 D+TLSV+EE CNDDEQ+QGG +D ISLEELN+K EEFIR  K  I+
Sbjct: 80  AKTVKGDKTLSVVEETCNDDEQKQGGSVDAISLEELNRKCEEFIRRMKNDIQ 127

BLAST of Sgr023718.1 vs. NCBI nr
Match: MBQ3811526.1 (hypothetical protein [Kiritimatiellae bacterium])

HSP 1 Score: 62.0 bits (149), Expect = 1.1e-05
Identity = 67/230 (29.13%), Postives = 117/230 (50.87%), Query Frame = 0

Query: 93  EVGDEKETGSLHVEHVREDQVLIAEDDDEKAGETLYIAVEEIYDDDCDGEV-AGDDDEKE 152
           EV D+ E      E V +D   + +D++E   +    AVEE  ++D +G +   ++D +E
Sbjct: 85  EVEDDSEEVEDDEEEVEDDAEEVEDDEEEVEDDAEDEAVEEDEEEDDEGYIFPEEEDTEE 144

Query: 153 IGSLRAVQVREDGVQIEEDDDEKAGETLYVVNEEIYDYDRNGEVAGEDDEKETRSLHVVQ 212
                  +V +D  ++EED++E   +T  V  +E    D   EV  +++E E  +  V +
Sbjct: 145 EVEEDEEEVEDDTEEVEEDEEEVEDDTEEVEEDEEEVEDDTEEVEEDEEEVEDDTEEVEE 204

Query: 213 VREDWVLIVEDNDEKAVETLYVVDEEIYDDDRDGEVAEDDKTLPVNVEESQEDELEVVED 272
             E+    VED+ E+  E   VV+++  + + D EV EDD       EE  ED+ E VE+
Sbjct: 205 DEEE----VEDDTEEVEEDEEVVEDDTEEVEEDEEVVEDDTEEVEEDEEEVEDDTEEVEE 264

Query: 273 DQTLSVIEEICND-DEQEQGGGIDTISLEELNKKFEEFIRTTKEQIRKAD 321
           D+     EEI ND DE E+   +D +  ++   + E+ +   +++I +AD
Sbjct: 265 DE-----EEIENDEDEIEEADPLDGLDEDDEIDEVEDDVEDDEDEIEEAD 305

BLAST of Sgr023718.1 vs. ExPASy TrEMBL
Match: A0A6J1CFL5 (uncharacterized protein LOC111011235 OS=Momordica charantia OX=3673 GN=LOC111011235 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 2.2e-52
Identity = 167/331 (50.45%), Postives = 206/331 (62.24%), Query Frame = 0

Query: 1   MKFFVSIFAVSITVFFFFSLSAAYSWIFYLSATIHKNYMFLFCNSLLVFICLNSRSNNGV 60
           MK F+SIFAVSITVFFF SLSAA+SW  YLSATIHK+YMFL CNSLLVFICLNS S   V
Sbjct: 1   MKIFISIFAVSITVFFFLSLSAAFSWTLYLSATIHKSYMFLLCNSLLVFICLNSPST--V 60

Query: 61  SSEKDYGQEVAEKKQAAGYYESRPLNRCRTVGEVGDEKETGSLHVEHVREDQVLIAEDDD 120
           +S                      L+  RTVGEVGDEKETGSLHV  V EDQ+LIAED+D
Sbjct: 61  NS----------------------LDGSRTVGEVGDEKETGSLHVVRVFEDQILIAEDND 120

Query: 121 EKAGETLYIAVEEIYDDDCDGEVAGDDDEKEIGSLRAVQVREDGVQIEEDDDEKAGETLY 180
           EKAGETL+  VEE  DD+                   ++VRED +            TL+
Sbjct: 121 EKAGETLF--VEETRDDE-------------------LEVREDDM------------TLF 180

Query: 181 VVNEEIYDYDRNGEVAGEDDEKETRSLHVVQVREDWVLIVEDNDEKA----VETLYVVDE 240
           V+ EEI+D         E + K+ R+L VV+       I +DN+++     V+ +  + E
Sbjct: 181 VI-EEIHD--------DELEAKDDRTLSVVEE------IHDDNEQEQGGGDVDMILSIVE 240

Query: 241 EIYDDDRDGEVAEDDKTLPVNVEESQEDELEVVEDDQTLSVIEEICNDD-EQEQGGGIDT 300
           E  DD+   EV EDD TL  +VEE+Q+DELE   DD+TLSV++E  +D+ EQEQGGG D 
Sbjct: 241 ETRDDEL--EVVEDDMTL-FDVEETQDDELEAA-DDKTLSVVKETHDDEQEQEQGGGNDM 255

Query: 301 ISLEELNKKFEEFIRTTKEQIRKADQTIQCH 327
           ISLEELNKKF+EFIRTT+ +IR    TIQC+
Sbjct: 301 ISLEELNKKFDEFIRTTRMKIRAELLTIQCY 255

BLAST of Sgr023718.1 vs. ExPASy TrEMBL
Match: A0A0A0KCP4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G111940 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 7.9e-10
Identity = 55/112 (49.11%), Postives = 73/112 (65.18%), Query Frame = 0

Query: 215 DWVLIVEDNDEKAVE---TLYVVDEEIYDDDRDGEVAEDDKTLPVNVEESQEDELEVVED 274
           D V + + N   A E   TL V+ E   D   + E+ +DD+TL V +EE+Q+ + +VVED
Sbjct: 20  DNVEVTQANQLDAPEEDMTLSVIKETKGD---ELELVKDDRTLSV-IEETQDYDSDVVED 79

Query: 275 ------DQTLSVIEEICNDDEQEQGGGIDTISLEELNKKFEEFIRTTKEQIR 318
                 D+TLSV+EE CNDDEQ+QGG +D ISLEELN+K EEFIR  K  I+
Sbjct: 80  AKTVKGDKTLSVVEETCNDDEQKQGGSVDAISLEELNRKCEEFIRRMKNDIQ 127

BLAST of Sgr023718.1 vs. ExPASy TrEMBL
Match: A0A072VM56 (Uncharacterized protein OS=Medicago truncatula OX=3880 GN=MTR_2g090985 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.2e-05
Identity = 60/222 (27.03%), Postives = 118/222 (53.15%), Query Frame = 0

Query: 97  EKETGSLHVEHVREDQVLIAEDDDEKAGETLYIAVEEIYDDDCDGEVAGDDDEKEIGSLR 156
           E+E      E V E +V + E+++E          EE+ +++ + EV  +++E+E+  + 
Sbjct: 287 EEEEEQEEEEEVEEVEVEVGEEEEE----------EEVKEEEVEEEVEVEEEEEEVEEVE 346

Query: 157 AVQV--------REDGVQIEEDDDEKAGETLYV-VNEEIYDYDRNGEVAGEDDEKETRSL 216
             +V         E+ V+ EE+++E   E   + V EE  + +   EV  E++E+E   +
Sbjct: 347 EEEVVEEEEEQEEEEEVEEEEEEEEVEDEVEEIEVEEEEVEEEEEEEVEVEEEEEEEEVV 406

Query: 217 HVVQVREDWVLIVEDNDEKAV--ETLYVVDEEIYDDDRDGEVAEDDKTLPVNVEESQEDE 276
            V +  E+ V++  + +E  V  E + V +EE  D++ + EV E ++   V  EE QE+E
Sbjct: 407 EVEEEEEEDVVVEVEEEEVEVEEEVVEVEEEEEEDEEEEEEVEEVEEEEVVEEEEEQEEE 466

Query: 277 LEVVEDDQTLSVIEEICNDDEQEQGGGIDTISLEELNKKFEE 308
            EV E+++   V +E+  ++E+E+   ++ I +EE  ++ EE
Sbjct: 467 EEVEEEEEEEEVEDEVEEEEEEEEVEEVEEIEVEEEEEEVEE 498

BLAST of Sgr023718.1 vs. ExPASy TrEMBL
Match: A0A267MKS4 (Cu_amine_oxidN1 domain-containing protein OS=Anaeromicrobium sediminis OX=1478221 GN=CCE28_06480 PE=4 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 2.2e-04
Identity = 63/243 (25.93%), Postives = 128/243 (52.67%), Query Frame = 0

Query: 93  EVGDEKETGSLHVEHVRE--DQVLIAEDDDEKAGETLYIAVEEIYDDDCDGEVAGDDDEK 152
           +V  + ETG++ ++   E  D+  I E+D+E++ E   +  +E  + D D +V  +D+E+
Sbjct: 278 KVDYDGETGTIEIDDEDEDADEDQIVEEDEEESDEDQVVEEDEEEESDED-QVVEEDEEE 337

Query: 153 EIGSLRAVQVRE----DGVQIEEDDDEKAGETLYVVNEEIYDYDRNGEVAGEDDEKETRS 212
           E    + V+  E    D  Q+ E+D+E+  +   VV E+  +     +V  ED+E+E+  
Sbjct: 338 ESDEDQVVEEDEEEESDEDQVVEEDEEEESDEDQVVEEDEEEESDEDQVVEEDEEEESDE 397

Query: 213 LHVVQVRE----DWVLIVEDNDEKAVETLYVVDEEIYDDDRDGEVAEDDKTLPVNVEESQ 272
             VV+  E    D   +VE+++E+  +   VV+E+  ++  + +V E+D+      EES 
Sbjct: 398 DQVVEEDEEEESDEDQVVEEDEEEESDEDQVVEEDEEEESDEDQVVEEDEE-----EESD 457

Query: 273 EDELEVVEDDQTLSVIEEICNDDEQEQGGGIDTISLEELNKKFE-EFIRTTKEQIRKADQ 325
           ED++ V ED++  S  +++  +DE+E+      +  +E  +  E + +   +E+    DQ
Sbjct: 458 EDQV-VEEDEEEESDEDQVVEEDEEEESDEDQVVEEDEEEESDEDQVVEEDEEEESDEDQ 513