Sgr023652.1 (mRNA) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr023652.1
Type	mRNA
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Auxin-responsive GH3 family protein
Location	tig00000892: 5266767 .. 5268788 (+)
Sequence length	1857
RNA-Seq Expression	Sgr023652.1
Synteny	Sgr023652.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTGGAGGCTTCAAACAACTTACCCTCGAACCTTCAAAGCTTTGATGCTCATTGTTCCCATCAGAAAGATGACCAGGCATTGCAGTATATTGAAGAAGTTACAACTCATGCCGATCAAGTTCAACGACGAGTTCTTACTGAAATTCTCTCGCGCAGTGCCCATGTCGAGTACCTGCAACGAAATGGCCTTAATGGATGTACAGATAGGAAGACGTTCAAGATGACGATGCCTCTAGTCTCTTATGAGGATTTAAAACCATACGTCGATCGAATTGCGAATGGCGATGCTTCTCCAATTCTATGTTCAAACCCCATATCAGAGTTCTTAACTAGGTGAGTATATAATCTATTTCATTTGCTCAAATGCATCGAGATCATAAATTCAATATAGCATTTTTCCATTGTAGTTCCGGAACATCGGGGGGAGAGAGGAAGTTAATGCCAACAACCGAAGAGGAGCTGGAGAGAAGATCATTTCTTTATTCGCTTCTGATGCCTGTGATGAACCAGTTCGTTCCAGGCCTGGACAAGGGCAAAGGAATGTACTTTTTGTTTGTAAAATCTGAGTCTACGACTCCAGGAGGGCTACTTGCTCGTCCAGTTCTCACCAGCTACTACAGAAGCTCACACTTTAAGGACCGCCCTTATAATACGTACAACGATTACACCAGCCCTAATGAAACAATTCTTTGTACAGATTCATACCAAAGCATGTACTCTCAGTTTCTTTGTGGACTCTGCCTCAGCAGGGGAGTAATTAGAGTTGGTGCTGTTTTTGCTTCTGGGTTCATTCGTGCCATCAAATTCCTTGAAAATCATTGGGCCCTTTTAGCCAATGATATCAGAACAGGAACCATAAACCCTAGAATCACTGACCCATTAGTGAGACAATCTGTCATGAACATTCTGAAACCTGACCCCATGCTTGCCGATTTAATTGAATCTGAGTGCAGAAAAGAAATGTGGGCAGGTATCATAACTAGGCTGTGGCCAAATACCAAGTATATTGACGTTATTGTGACAGGTACTATGTCACAGTACATCCCCACTTTAGATTACTATAGTAATGGCCTCCCTCTTGTTTGTACTATGTATGCTTCTTCCGAGTGCTACTTCGGCATCAATCTAAATCCCTTGTGTAAGCCAAACGAGGTCTCTTACACCCTCATTCCCACCATGGCGTATTTCGAATTTTTGCCTCTCGACAGGACAAATGGATTCAGCAACTCAGCTTGTGAATCAGAAGCCAATGTGAAAATAAACGAAGAATTGGTTGATCTGGTGGATGTCGAATTAGGAAAAGAATATGAGCTCGTTATCACCACTTATGCAGGTAAGTATTGAAGTAGACTTCAAACGTAATGTAAAGGATTGTTTGGTTTATGTATGAATAAATTGGGGATAATCTTATCGGATATTTGCAGGCCTCTATCGGTATCGAGTTGGCGACATTCTTCGAGTATCAGGATTCAAGAACAACGCCCCACAATTGAACTTCGTATGCAGGAAAAATGTGATTCTTAGTATTGATTCCGACAAAGTTGACGAAGTGGAGCTTCAGAACGCAGTAATGGAAGCTTCTAAAAACTTGATTCCATACTGCGCTTGTCTCCTCGAGTACACGAGCTATGCTGATACTTGTACAATTCCAGGTCACTACGTCATATATTGGGAGATTAGCCTAAAGGAAGCGACCGCTAATATTCCTCGTATTGTATTTGAAAAATGTTGTCTAGCAATGGAAGAATCACTCAACAGCGTATATCGTCAAGGAAGAGTCTCAGACAAATCTATTGGTCCTTTGGAGATGAAGATTGTGGAGCCTGGAACGTTCGACAAACTAATGGACTATGCCCTCAATCATGGTGCTTCCATAAACCAATACAAGACACCTCGCTGCGTGAAACATGCACCAATTCTTGAGCTCTTGAACTCGTACGTTGTGTCGAATTATTTCAGCCCAAAATGTCCGAACTGGGTTCCTGGTCGTAAAGAATGGTCCACCCAGAGAACCGAATGA

mRNA sequence

ATGCTGGAGGCTTCAAACAACTTACCCTCGAACCTTCAAAGCTTTGATGCTCATTGTTCCCATCAGAAAGATGACCAGGCATTGCAGTATATTGAAGAAGTTACAACTCATGCCGATCAAGTTCAACGACGAGTTCTTACTGAAATTCTCTCGCGCAGTGCCCATGTCGAGTACCTGCAACGAAATGGCCTTAATGGATGTACAGATAGGAAGACGTTCAAGATGACGATGCCTCTAGTCTCTTATGAGGATTTAAAACCATACGTCGATCGAATTGCGAATGGCGATGCTTCTCCAATTCTATGTTCAAACCCCATATCAGAGTTCTTAACTAGTTCCGGAACATCGGGGGGAGAGAGGAAGTTAATGCCAACAACCGAAGAGGAGCTGGAGAGAAGATCATTTCTTTATTCGCTTCTGATGCCTGTGATGAACCAGTTCGTTCCAGGCCTGGACAAGGGCAAAGGAATGTACTTTTTGTTTGTAAAATCTGAGTCTACGACTCCAGGAGGGCTACTTGCTCGTCCAGTTCTCACCAGCTACTACAGAAGCTCACACTTTAAGGACCGCCCTTATAATACGTACAACGATTACACCAGCCCTAATGAAACAATTCTTTGTACAGATTCATACCAAAGCATGTACTCTCAGTTTCTTTGTGGACTCTGCCTCAGCAGGGGAGTAATTAGAGTTGGTGCTGTTTTTGCTTCTGGGTTCATTCGTGCCATCAAATTCCTTGAAAATCATTGGGCCCTTTTAGCCAATGATATCAGAACAGGAACCATAAACCCTAGAATCACTGACCCATTAGTGAGACAATCTGTCATGAACATTCTGAAACCTGACCCCATGCTTGCCGATTTAATTGAATCTGAGTGCAGAAAAGAAATGTGGGCAGGTATCATAACTAGGCTGTGGCCAAATACCAAGTATATTGACGTTATTGTGACAGGTACTATGTCACAGTACATCCCCACTTTAGATTACTATAGTAATGGCCTCCCTCTTGTTTGTACTATGTATGCTTCTTCCGAGTGCTACTTCGGCATCAATCTAAATCCCTTGTGTAAGCCAAACGAGGTCTCTTACACCCTCATTCCCACCATGGCGTATTTCGAATTTTTGCCTCTCGACAGGACAAATGGATTCAGCAACTCAGCTTGTGAATCAGAAGCCAATGTGAAAATAAACGAAGAATTGGTTGATCTGGTGGATGTCGAATTAGGAAAAGAATATGAGCTCGTTATCACCACTTATGCAGGCCTCTATCGGTATCGAGTTGGCGACATTCTTCGAGTATCAGGATTCAAGAACAACGCCCCACAATTGAACTTCGTATGCAGGAAAAATGTGATTCTTAGTATTGATTCCGACAAAGTTGACGAAGTGGAGCTTCAGAACGCAGTAATGGAAGCTTCTAAAAACTTGATTCCATACTGCGCTTGTCTCCTCGAGTACACGAGCTATGCTGATACTTGTACAATTCCAGGTCACTACGTCATATATTGGGAGATTAGCCTAAAGGAAGCGACCGCTAATATTCCTCGTATTGTATTTGAAAAATGTTGTCTAGCAATGGAAGAATCACTCAACAGCGTATATCGTCAAGGAAGAGTCTCAGACAAATCTATTGGTCCTTTGGAGATGAAGATTGTGGAGCCTGGAACGTTCGACAAACTAATGGACTATGCCCTCAATCATGGTGCTTCCATAAACCAATACAAGACACCTCGCTGCGTGAAACATGCACCAATTCTTGAGCTCTTGAACTCGTACGTTGTGTCGAATTATTTCAGCCCAAAATGTCCGAACTGGGTTCCTGGTCGTAAAGAATGGTCCACCCAGAGAACCGAATGA

Coding sequence (CDS)

ATGCTGGAGGCTTCAAACAACTTACCCTCGAACCTTCAAAGCTTTGATGCTCATTGTTCCCATCAGAAAGATGACCAGGCATTGCAGTATATTGAAGAAGTTACAACTCATGCCGATCAAGTTCAACGACGAGTTCTTACTGAAATTCTCTCGCGCAGTGCCCATGTCGAGTACCTGCAACGAAATGGCCTTAATGGATGTACAGATAGGAAGACGTTCAAGATGACGATGCCTCTAGTCTCTTATGAGGATTTAAAACCATACGTCGATCGAATTGCGAATGGCGATGCTTCTCCAATTCTATGTTCAAACCCCATATCAGAGTTCTTAACTAGTTCCGGAACATCGGGGGGAGAGAGGAAGTTAATGCCAACAACCGAAGAGGAGCTGGAGAGAAGATCATTTCTTTATTCGCTTCTGATGCCTGTGATGAACCAGTTCGTTCCAGGCCTGGACAAGGGCAAAGGAATGTACTTTTTGTTTGTAAAATCTGAGTCTACGACTCCAGGAGGGCTACTTGCTCGTCCAGTTCTCACCAGCTACTACAGAAGCTCACACTTTAAGGACCGCCCTTATAATACGTACAACGATTACACCAGCCCTAATGAAACAATTCTTTGTACAGATTCATACCAAAGCATGTACTCTCAGTTTCTTTGTGGACTCTGCCTCAGCAGGGGAGTAATTAGAGTTGGTGCTGTTTTTGCTTCTGGGTTCATTCGTGCCATCAAATTCCTTGAAAATCATTGGGCCCTTTTAGCCAATGATATCAGAACAGGAACCATAAACCCTAGAATCACTGACCCATTAGTGAGACAATCTGTCATGAACATTCTGAAACCTGACCCCATGCTTGCCGATTTAATTGAATCTGAGTGCAGAAAAGAAATGTGGGCAGGTATCATAACTAGGCTGTGGCCAAATACCAAGTATATTGACGTTATTGTGACAGGTACTATGTCACAGTACATCCCCACTTTAGATTACTATAGTAATGGCCTCCCTCTTGTTTGTACTATGTATGCTTCTTCCGAGTGCTACTTCGGCATCAATCTAAATCCCTTGTGTAAGCCAAACGAGGTCTCTTACACCCTCATTCCCACCATGGCGTATTTCGAATTTTTGCCTCTCGACAGGACAAATGGATTCAGCAACTCAGCTTGTGAATCAGAAGCCAATGTGAAAATAAACGAAGAATTGGTTGATCTGGTGGATGTCGAATTAGGAAAAGAATATGAGCTCGTTATCACCACTTATGCAGGCCTCTATCGGTATCGAGTTGGCGACATTCTTCGAGTATCAGGATTCAAGAACAACGCCCCACAATTGAACTTCGTATGCAGGAAAAATGTGATTCTTAGTATTGATTCCGACAAAGTTGACGAAGTGGAGCTTCAGAACGCAGTAATGGAAGCTTCTAAAAACTTGATTCCATACTGCGCTTGTCTCCTCGAGTACACGAGCTATGCTGATACTTGTACAATTCCAGGTCACTACGTCATATATTGGGAGATTAGCCTAAAGGAAGCGACCGCTAATATTCCTCGTATTGTATTTGAAAAATGTTGTCTAGCAATGGAAGAATCACTCAACAGCGTATATCGTCAAGGAAGAGTCTCAGACAAATCTATTGGTCCTTTGGAGATGAAGATTGTGGAGCCTGGAACGTTCGACAAACTAATGGACTATGCCCTCAATCATGGTGCTTCCATAAACCAATACAAGACACCTCGCTGCGTGAAACATGCACCAATTCTTGAGCTCTTGAACTCGTACGTTGTGTCGAATTATTTCAGCCCAAAATGTCCGAACTGGGTTCCTGGTCGTAAAGAATGGTCCACCCAGAGAACCGAATGA

Protein sequence

MLEASNNLPSNLQSFDAHCSHQKDDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDRKTFKMTMPLVSYEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLYSLLMPVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYNDYTSPNETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDIRTGTINPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPLDRTNGFSNSACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIPGHYVIYWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRKEWSTQRTE

Homology

[Close]

BLAST of Sgr023652.1 vs. NCBI nr
Match: XP_022140599.1 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 [Momordica charantia])

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 541/622 (86.98%), Postives = 569/622 (91.48%), Query Frame = 0


Query: 1   MLEASNNLPSNLQSFDAHCSHQKDDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQ 60
           M EASNNLP    S     SH+K ++ALQYIE+VT HADQVQRRVLTEILS +AHVEYL 
Sbjct: 1   MPEASNNLPPEPDSL-IGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL- 60

Query: 61  RNGLNGCTDRKTFKMTMPLVSYEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGER 120
           R+GL+G  D KTFK TMP+VSY+D+KPYVDRIANGD SPILCS PISEFLTSSGTSGGER
Sbjct: 61  RHGLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGER 120

Query: 121 KLMPTTEEELERRSFLYSLLMPVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTS 180
           KLMPTTEEELER+S LYSLLMPVMNQF+PGLDKGKGMYFLFVKSESTTPGGLLARPVLTS
Sbjct: 121 KLMPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTS 180

Query: 181 YYRSSHFKDRPYNTYNDYTSPNETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFI 240
           YYRS HFKDRPY+ YN YTSPNETILCTDSYQSMYSQFLCGLCLSR V+RVGAVFASGFI
Sbjct: 181 YYRSPHFKDRPYDPYNQYTSPNETILCTDSYQSMYSQFLCGLCLSREVLRVGAVFASGFI 240

Query: 241 RAIKFLENHWALLANDIRTGTINPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAG 300
           RAIKFLE HW LL+NDIRTGTINPRITDPLVRQSV+NILKPDP LADLIESEC K  W G
Sbjct: 241 RAIKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEG 300

Query: 301 IITRLWPNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNE 360
           II+RLW NTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLC P+E
Sbjct: 301 IISRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSE 360

Query: 361 VSYTLIPTMAYFEFLPLDRTNGFS-NSACESEANVKINEELVDLVDVELGKEYELVITTY 420
           VSYTLIPTMAYFEFLPLD TNGF  +S C+SEAN K ++ELVDLVDV+LGKEYELVITTY
Sbjct: 361 VSYTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTY 420

Query: 421 AGLYRYRVGDILRVSGFKNNAPQLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY 480
           AGLYRYRVGDILRVSGFKNNAPQ NFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY
Sbjct: 421 AGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY 480

Query: 481 CACLLEYTSYADTCTIPGHYVIYWEISLKEATAN----IPRIVFEKCCLAMEESLNSVYR 540
            AC++EYTSYADT TIPGHYVIYWEISLKEATAN    IP  VFEKCCL+MEESLNSVYR
Sbjct: 481 GACVVEYTSYADTVTIPGHYVIYWEISLKEATANNNNVIPGSVFEKCCLSMEESLNSVYR 540

Query: 541 QGRVSDKSIGPLEMKIVEPGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVVS 600
           QGRVSDKSIGPLEMKIVE GTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVV 
Sbjct: 541 QGRVSDKSIGPLEMKIVETGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVVD 600

Query: 601 NYFSPKCPNWVPGRKEWSTQRT 618
           NYFSPKCP+WVPGRKEWST  +
Sbjct: 601 NYFSPKCPHWVPGRKEWSTNNS 620

[Close]

BLAST of Sgr023652.1 vs. NCBI nr
Match: XP_022948165.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 1034.2 bits (2673), Expect = 4.4e-298
Identity = 505/595 (84.87%), Postives = 541/595 (90.92%), Query Frame = 0


Query: 23  KDDQA-LQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDRKTFKMTMPLVS 82
           KD+ A LQ+IEE+TTHADQVQRRVLTEILSR+AHVEYLQ+ GL+GC DR  FK  +P+VS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLYSLLM 142
           YE+LKPY+DRIANGDASPILCS P+SEFLTSSGTSGGERKLMPTTEEEL+RRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 123

Query: 143 PVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYNDYTSP 202
           PVMNQF+PGL+KGKGMYFLFVKSESTTPGGL ARPVLTSYY+SSHFKDRPY+ Y DYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDIRTGT 262
           N+TILCTDSYQSMY QFLCGLCLS+ V+RVG+VFASGFIRAIKFLE HW LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVTGTMS 322
           INPRI DPLVRQSVMNILKPDP+LADLIE EC K  WAGII RLWPNTKYIDVIVTGTMS
Sbjct: 244 INPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMS 303

Query: 323 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPLDRTN 382
           QYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC PNEVSYTLIPTMAYFEFLPLDR  
Sbjct: 304 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFSNSACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAP 442
                        K++EELVDLVDVELGKEYELVITTY+GLYRYRVGDILRVSGFKN AP
Sbjct: 364 -------------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAP 423

Query: 443 QLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIPGHYVI 502
           Q NFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY   LLEYTSYADT T+PGHYVI
Sbjct: 424 QFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVI 483

Query: 503 YWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGTFDKLM 562
           YWEIS KE  A IP +VFE CCL +EESLNSVYRQGRVSDKSIGPLE+KI+E GTFDKLM
Sbjct: 484 YWEISQKETNA-IPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLM 543

Query: 563 DYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRKEWSTQR 617
           DYA+NHG+SINQYKTPRCVKH PILELLNSYVVSNYFSP+CP WVPG+KEWST++
Sbjct: 544 DYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTKK 582

[Close]

BLAST of Sgr023652.1 vs. NCBI nr
Match: KAG6604833.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1033.9 bits (2672), Expect = 5.7e-298
Identity = 504/595 (84.71%), Postives = 542/595 (91.09%), Query Frame = 0


Query: 23  KDDQA-LQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDRKTFKMTMPLVS 82
           KD+ A LQ+IEE+TTHADQVQRRVLTEILSR+AHVEYLQ+ GL+GC DR  FK  +P+VS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLYSLLM 142
           YE+LKPY+DRIANGDASPILCS P+SEFLTSSGTSGGERKLMPTT+EEL+RRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTKEELKRRSFLYSLLM 123

Query: 143 PVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYNDYTSP 202
           PVMNQF+PGL+KGKGMYFLFVKSESTTPGGL ARPVLTSYY+SSHFKDRPY+ Y DYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDIRTGT 262
           N+TILCTDSYQSMY QFLCGLCLS+ V+RVG+VFASGFIRAIKFLE HW LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVTGTMS 322
           INPRI+DPLVRQSVMNILKPDP+LADLIE EC K  WAGII RLWPNTKYIDVIVTGTMS
Sbjct: 244 INPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMS 303

Query: 323 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPLDRTN 382
           QYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC PNEVSYTLIPTMAYFEFLPLDR  
Sbjct: 304 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFSNSACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAP 442
                        K++EELVDLVDVELGKEYELVITTY+GLYRYRVGDILRVSGFKN AP
Sbjct: 364 -------------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAP 423

Query: 443 QLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIPGHYVI 502
           Q NFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY   LLEYTSYADT T+PGHYVI
Sbjct: 424 QFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVI 483

Query: 503 YWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGTFDKLM 562
           YWEIS KE  A IP +VFE CCL +EESLNSVYRQGRVSDKSIGPLE+KI+E GTFDKLM
Sbjct: 484 YWEISQKETNA-IPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLM 543

Query: 563 DYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRKEWSTQR 617
           DYA+NHG+SINQYKTPRCVKH PILELLNSYVVSNYFSP+CP WVPG+KEWST++
Sbjct: 544 DYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGKKEWSTKK 582

[Close]

BLAST of Sgr023652.1 vs. NCBI nr
Match: XP_023533206.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1031.9 bits (2667), Expect = 2.2e-297
Identity = 501/596 (84.06%), Postives = 544/596 (91.28%), Query Frame = 0


Query: 22  QKDDQA-LQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDRKTFKMTMPLV 81
           ++D+ A LQ+IEE+TTHADQVQRRVLTEILSR+AHVEYLQ+ GL+GC DR  FK  +P+V
Sbjct: 10  ERDEGAILQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVV 69

Query: 82  SYEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLYSLL 141
           SYE+LKPY+DRIANGDASPILCS P+SEFLTSSGTSGGERKLMPTTEEEL+RRSFLYSLL
Sbjct: 70  SYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLL 129

Query: 142 MPVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYNDYTS 201
           MPVMNQF+PGL++GKGMYFLFVKSESTTPGGL ARPVLTSYY+SSHFKDRPY+ Y DYTS
Sbjct: 130 MPVMNQFIPGLNRGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTS 189

Query: 202 PNETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDIRTG 261
           PN+TILCTDSYQSMYSQFLCGLCLS+ V+RVG+VFASGFIRAIKFLE HW LLANDIRTG
Sbjct: 190 PNQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTG 249

Query: 262 TINPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVTGTM 321
           TINPRI+DPLVRQSVMNILKPDP+LADLIE EC K  WAGII RLWPNTKYIDVIVTGTM
Sbjct: 250 TINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTM 309

Query: 322 SQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPLDRT 381
           SQYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC PNEVSYTLIPTMAYFEFLPLDR 
Sbjct: 310 SQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR- 369

Query: 382 NGFSNSACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNA 441
                         +++EELVDLVDVELGKEYELVITTY+GLYRYRVGDILRVSGFKN A
Sbjct: 370 --------------EMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKA 429

Query: 442 PQLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIPGHYV 501
           PQ NFVCRKNVILSIDSDKVDEVELQNAVMEASKNL+PY   LLEYTSYADT T+PGHYV
Sbjct: 430 PQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLVPYETSLLEYTSYADTHTVPGHYV 489

Query: 502 IYWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGTFDKL 561
           IYWEIS KE  A IP +VFE CCL +EESLNSVYRQGRVSDKSIGPLE+KI+E GTFDKL
Sbjct: 490 IYWEISQKETNA-IPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKL 549

Query: 562 MDYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRKEWSTQR 617
           MDYA+NHG+SINQYKTPRCVKH PILELLNSYVVSNYFSP+CP WVPG+KEWS+++
Sbjct: 550 MDYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSSKK 589

[Close]

BLAST of Sgr023652.1 vs. NCBI nr
Match: XP_022970970.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima])

HSP 1 Score: 1030.4 bits (2663), Expect = 6.4e-297
Identity = 504/595 (84.71%), Postives = 541/595 (90.92%), Query Frame = 0


Query: 23  KDDQA-LQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDRKTFKMTMPLVS 82
           KD+ A LQ+IEE+TTHADQVQRRVLTEILSR+AHVEYLQ+ GL+GC DR  FK  +P+VS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLYSLLM 142
           YE+LKPY+DRIANGDASPILCS PISEFLTSSGTSGGERKLMPTT+EEL+RRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPISEFLTSSGTSGGERKLMPTTKEELKRRSFLYSLLM 123

Query: 143 PVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYNDYTSP 202
           PVMNQF+PGL+KGKGMYFLFVKSESTTPGGL ARPVLTSYY+SSHFKDRPY+ Y DYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDIRTGT 262
           N+TILCTDSYQSMYSQFLCGLCLS+ V+RVG+VFASGFIRAIKFLE HW LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVTGTMS 322
           INPRI+DPLVRQSVMNILKPDP+LADLIE EC K  WAGIITRLWPNTKY+DVIVTGTMS
Sbjct: 244 INPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIITRLWPNTKYVDVIVTGTMS 303

Query: 323 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPLDRTN 382
           QYIPTLD YSNGLPLVCTMYASSECYFG+NLNPLC PNEVSYTLIPTMAYFEFLPLDR  
Sbjct: 304 QYIPTLDKYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFSNSACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAP 442
                        K++EELVDLVDVELGKEYEL+ITTY+GLYRYRVGDILRVSGFKN AP
Sbjct: 364 -------------KMSEELVDLVDVELGKEYELIITTYSGLYRYRVGDILRVSGFKNKAP 423

Query: 443 QLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIPGHYVI 502
           Q NFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY   LLEYTSYADT T+PGHYVI
Sbjct: 424 QFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVI 483

Query: 503 YWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGTFDKLM 562
           YWEIS KE  A IP  VFE CCL +EESLNSVYRQGRVSDKSIGPLE+KI+E GTFDKLM
Sbjct: 484 YWEISQKETNA-IPDWVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLM 543

Query: 563 DYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRKEWSTQR 617
           DYA+NHG+SINQYKTPRCVK  PILELLNSYVVSNYFSPKCP WVPG+KEWST++
Sbjct: 544 DYAINHGSSINQYKTPRCVKREPILELLNSYVVSNYFSPKCPKWVPGQKEWSTKK 582

[Close]

BLAST of Sgr023652.1 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 4.9e-268
Identity = 447/594 (75.25%), Postives = 512/594 (86.20%), Query Frame = 0


Query: 22  QKDDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDRKTFKMTMPLVS 81
           +K+   LQ+IE+VTT+AD VQRRVL EILSR+A VEYL+R+GL G TDR+TFK  MP+V+
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 82  YEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLYSLLM 141
           YED++P ++RIANGD S +LCSNPISEFLTSSGTSGGERKLMPT EEEL+RRS LYSLLM
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 142 PVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYNDYTSP 201
           PVM+QFVPGLDKGKGMYFLF+KSES TPGGL ARPVLTSYY+SSHFK+RPY+ Y +YTSP
Sbjct: 139 PVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSP 198

Query: 202 NETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDIRTGT 261
           N+TILC+DSYQSMYSQ LCGLC  + V+RVGAVFASGFIRAIKFLE HW  LA DIRTGT
Sbjct: 199 NQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGT 258

Query: 262 INPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVTGTMS 321
           ++  ITD  VR++V  ILKPDP LAD +ESECRK  W GIITRLWPNTKY+DVIVTGTMS
Sbjct: 259 LSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMS 318

Query: 322 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPLDRTN 381
           QYIPTLDYYSNGLPLVCTMYASSECYFG+NL PLCKP+EVSYTLIP MAYFEFLP+ R +
Sbjct: 319 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNS 378

Query: 382 GFSNSACESEA-NVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNA 441
           G ++S    +A   K  +ELVDLVDV+LG+EYELV+TTYAGLYRYRVGD+L V+GFKNNA
Sbjct: 379 GVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNA 438

Query: 442 PQLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIPGHYV 501
           PQ +F+CRKNV+LSIDSDK DEVELQNAV  A  +L+P+ A L EYTSYADT +IPGHYV
Sbjct: 439 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 498

Query: 502 IYWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGTFDKL 561
           ++WE+ L   T  IP  VFE CCL +EESLNSVYRQGRVSDKSIGPLE+K+VE GTFDKL
Sbjct: 499 LFWELCLNGNTP-IPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKL 558

Query: 562 MDYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRKEWST 615
           MDYA++ GASINQYKTPRCVK API+ELLNS VV +YFSPKCP W PG K+W +
Sbjct: 559 MDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQWGS 611

[Close]

BLAST of Sgr023652.1 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 2.7e-266
Identity = 447/605 (73.88%), Postives = 516/605 (85.29%), Query Frame = 0


Query: 11  NLQSFDAHCSHQKDDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDR 70
           +L+ FD     QK+ Q LQ IEE+T++ADQVQR+VL EIL+R+A VEYL+R+ LNG TDR
Sbjct: 9   SLEVFDLTLD-QKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDR 68

Query: 71  KTFKMTMPLVSYEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEEL 130
           +TFK  MP+++YED++P ++RIANGD SPIL S PISEFLTSSGTSGGERKLMPT EEEL
Sbjct: 69  ETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEEL 128

Query: 131 ERRSFLYSLLMPVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDR 190
           +RRS LYSLLMPVM+QFVPGL+ GKGMYFLF+KSES TPGGL ARPVLTSYY+SSHFK+R
Sbjct: 129 DRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKER 188

Query: 191 PYNTYNDYTSPNETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHW 250
           PY+ Y +YTSPNETILC+DSYQSMYSQ LCGLC  + V+RVGAVFASGFIRAIKFLE HW
Sbjct: 189 PYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHW 248

Query: 251 ALLANDIRTGTINPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTK 310
             L  DIRTGT++  ITDP VR++V  ILKP P LAD +E EC+K  W GIITRLWPNTK
Sbjct: 249 IELVRDIRTGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTK 308

Query: 311 YIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMA 370
           Y+DVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFG+NL PLCKP+EVSYTLIP+MA
Sbjct: 309 YVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMA 368

Query: 371 YFEFLPLDRTNGFSNSACESEA-NVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGD 430
           YFEFLP+ R NG +NS    +A   K  +ELVDLVDV+LG+EYELV+TTYAGL RYRVGD
Sbjct: 369 YFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGD 428

Query: 431 ILRVSGFKNNAPQLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSY 490
           +LRV+GFKN APQ +F+CRKNV+LSIDSDK DEVELQNAV  A  +L+P+ A L EYTSY
Sbjct: 429 LLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSY 488

Query: 491 ADTCTIPGHYVIYWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEM 550
           ADT +IPGHYV++WE+ L +    IP  VFE CCLA+EES N+VYRQGRVSDKSIGPLE+
Sbjct: 489 ADTSSIPGHYVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEI 548

Query: 551 KIVEPGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGR 610
           KIVEPGTFDKLMDYA++ GASINQYKTPRCVK API+ELLNS VV +YFSPKCP WVPG 
Sbjct: 549 KIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGH 608

Query: 611 KEWST 615
           K+W +
Sbjct: 609 KQWGS 611

[Close]

BLAST of Sgr023652.1 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 2.1e-226
Identity = 380/592 (64.19%), Postives = 461/592 (77.87%), Query Frame = 0


Query: 26  QALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTD-RKTFKMTMPLVSYED 85
           +AL++IE VT +A QVQRRVL EIL+++A  EYL+R G+ G  D    F+  +PLV+YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 86  LKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLYSLLMPVM 145
           L+P + RIANGD SPI    PISEFLTSSGTSGGERKLMPT  +E+ RRS LYSLLMPVM
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 146 NQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYNDYTSPNET 205
           +Q V GLDKGK MY LFVK+ES TPGGL ARPVLTSYYRS  F DRP + Y  YTSP+E 
Sbjct: 142 SQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEA 201

Query: 206 ILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDIRTGTINP 265
           ILC DSYQSMY+Q LCGL     V+RVGAVFASGF+RAI FLE HWA L +DIRTG ++P
Sbjct: 202 ILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDP 261

Query: 266 RITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVTGTMSQYI 325
            ITD +VR +V  +L+ DP LAD IE EC +  W GII RLWP TKYIDVIVTGTMSQYI
Sbjct: 262 EITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYI 321

Query: 326 PTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPLDRTNGFS 385
           PTL++Y  GLPL CTMYASSECYFG+NLNP+CKP++V+YTLIPTM Y+EFLP++  N  +
Sbjct: 322 PTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATA 381

Query: 386 NSACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQLN 445
            ++         + +LVDLVDV+LG EYELV+TTY+GLYRYRVGD+LRV+GFKN AP  +
Sbjct: 382 EAS---------HRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFS 441

Query: 446 FVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIPGHYVIYWE 505
           FV R+NV LS+DSDK DE EL  AV  A ++L P+ A L+EYTSYAD  TIPGHYV++WE
Sbjct: 442 FVRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWE 501

Query: 506 ISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGTFDKLMDYA 565
             L+  +  +P  VFE+CCL++EE+LNSVYRQGR  D+SIGPLE+++V  GTFDKLMDYA
Sbjct: 502 --LRAGSTAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYA 561

Query: 566 LNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRKEWSTQR 617
           ++ GASINQYK PRCV+  P++ELL++ V   YFSPKCP W PG K+W+  +
Sbjct: 562 ISRGASINQYKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNKQWNKSK 602

[Close]

BLAST of Sgr023652.1 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 7.5e-224
Identity = 380/602 (63.12%), Postives = 463/602 (76.91%), Query Frame = 0


Query: 10  SNLQSFDAHCSHQKDDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTD 69
           SNL S     + +KD +AL++IEE+T +AD VQ  +L EIL+R+A  EYL+R  L G TD
Sbjct: 5   SNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATD 64

Query: 70  RKTFKMTMPLVSYEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEE 129
           R TFK  +P+++YEDL+P + RIA+GD SPIL ++PISEFLTSSGTS GERKLMPT +EE
Sbjct: 65  RDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEE 124

Query: 130 LERRSFLYSLLMPVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKD 189
           L+RR  LYSLLMPVMN +VPGLDKGKGMYFLFVKSE+ TPGGL ARPVLTSYY+S HF+ 
Sbjct: 125 LDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRS 184

Query: 190 RPYNTYNDYTSPNETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENH 249
           RPY+ YN YTSPNE ILC DS+QSMY+Q LCGL     V+RVGAVFASG +RAI+FL+ H
Sbjct: 185 RPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLH 244

Query: 250 WALLANDIRTGTINPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNT 309
           W+  A+DI  G ++  ITDP +RQ +  ILKPDP+LA+ I  EC+ + W  IITR+WPNT
Sbjct: 245 WSRFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNT 304

Query: 310 KYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTM 369
           KY+DVIVTG M+QYIPTL+YYS GLP+ CTMYASSECYFG+NLNP+ KP+EVSYT++P M
Sbjct: 305 KYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNM 364

Query: 370 AYFEFLPLDRTNGFSNSACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGD 429
           AYFEF+PL  T                  + V+LVDV +GKEYELV+TTYAGL RYRVGD
Sbjct: 365 AYFEFIPLGGT------------------KAVELVDVNIGKEYELVVTTYAGLCRYRVGD 424

Query: 430 ILRVSGFKNNAPQLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCAC-LLEYTS 489
           ILRV+GF N+APQ +FV RKNV+LSIDSDK DE ELQ AV  AS  L   C   + EYTS
Sbjct: 425 ILRVTGFHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTS 484

Query: 490 YADTCTIPGHYVIYWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLE 549
           YADT TIPGHYV+YWE+ +++           +CCL MEESLNSVYRQ RV+D S+GPLE
Sbjct: 485 YADTSTIPGHYVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLE 544

Query: 550 MKIVEPGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPG 609
           +++V  GTF++LMDYA++ GASINQYK PRCV   PI+ELL+S VVS +FSP  P+W P 
Sbjct: 545 IRVVRNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPE 588

Query: 610 RK 611
           R+
Sbjct: 605 RR 588

[Close]

BLAST of Sgr023652.1 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 777.7 bits (2007), Expect = 9.7e-224
Identity = 375/588 (63.78%), Postives = 461/588 (78.40%), Query Frame = 0


Query: 24  DDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDRKTFKMTMPLVSYE 83
           D + L++I+E+TT+ D VQ RVL EIL R+A  EYL + GL+G TDR  F+  +P+VSY+
Sbjct: 26  DVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYD 85

Query: 84  DLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLYSLLMPV 143
           DL+PY+ RIANGD SPIL ++P+SEFLTSSGTS GERKLMPT  +EL+RR  LYSLLMPV
Sbjct: 86  DLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPV 145

Query: 144 MNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYNDYTSPNE 203
           MN +VPGLDKGKG+YFLFVKSE+ TPGGL ARPVLTSYY+S HFK+RPY+ Y++YTSP  
Sbjct: 146 MNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTA 205

Query: 204 TILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDIRTGTIN 263
            ILC D++QSMY+Q +CGLC    V+R+GAVFASG +RAI+FL+ +W  LA+DI +G + 
Sbjct: 206 AILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELT 265

Query: 264 PRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVTGTMSQY 323
           PR+TDP VR++V  IL PDP LA LI +EC K  WAGIITR+WPNTKY+DVIVTG M+QY
Sbjct: 266 PRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQY 325

Query: 324 IPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPLDRTNGF 383
           IPTL++YS GLP+ CTMYASSECYFG+NL P+C P+EVSYT++P M YFEFLP+D T   
Sbjct: 326 IPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAA 385

Query: 384 SNSACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQL 443
           S  A           +LVDL  VE+G+EYELVITTYAGL RYRVGD+LRV+GF N APQ 
Sbjct: 386 SGDA----------TQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQF 445

Query: 444 NFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIPGHYVIYW 503
            FV RKNV+LSI+SDK DE ELQ AV  AS  L P+ A ++EYTS A T  IPGHYVIYW
Sbjct: 446 RFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYW 505

Query: 504 EISLKEATAN-IPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGTFDKLMD 563
           E+  K A A  +      +CCL MEE+LN+VYRQ RV+D SIGPLE+++V PGTF++LMD
Sbjct: 506 ELLTKGAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMD 565

Query: 564 YALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRK 611
           YA++ GASINQYK PRCV   PI+ELL+S VVS++FSP  P+W P R+
Sbjct: 566 YAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

[Close]

BLAST of Sgr023652.1 vs. ExPASy TrEMBL
Match: A0A6J1CG47 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011212 PE=3 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 541/622 (86.98%), Postives = 569/622 (91.48%), Query Frame = 0


Query: 1   MLEASNNLPSNLQSFDAHCSHQKDDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQ 60
           M EASNNLP    S     SH+K ++ALQYIE+VT HADQVQRRVLTEILS +AHVEYL 
Sbjct: 1   MPEASNNLPPEPDSL-IGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL- 60

Query: 61  RNGLNGCTDRKTFKMTMPLVSYEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGER 120
           R+GL+G  D KTFK TMP+VSY+D+KPYVDRIANGD SPILCS PISEFLTSSGTSGGER
Sbjct: 61  RHGLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGER 120

Query: 121 KLMPTTEEELERRSFLYSLLMPVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTS 180
           KLMPTTEEELER+S LYSLLMPVMNQF+PGLDKGKGMYFLFVKSESTTPGGLLARPVLTS
Sbjct: 121 KLMPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTS 180

Query: 181 YYRSSHFKDRPYNTYNDYTSPNETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFI 240
           YYRS HFKDRPY+ YN YTSPNETILCTDSYQSMYSQFLCGLCLSR V+RVGAVFASGFI
Sbjct: 181 YYRSPHFKDRPYDPYNQYTSPNETILCTDSYQSMYSQFLCGLCLSREVLRVGAVFASGFI 240

Query: 241 RAIKFLENHWALLANDIRTGTINPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAG 300
           RAIKFLE HW LL+NDIRTGTINPRITDPLVRQSV+NILKPDP LADLIESEC K  W G
Sbjct: 241 RAIKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEG 300

Query: 301 IITRLWPNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNE 360
           II+RLW NTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLC P+E
Sbjct: 301 IISRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSE 360

Query: 361 VSYTLIPTMAYFEFLPLDRTNGFS-NSACESEANVKINEELVDLVDVELGKEYELVITTY 420
           VSYTLIPTMAYFEFLPLD TNGF  +S C+SEAN K ++ELVDLVDV+LGKEYELVITTY
Sbjct: 361 VSYTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTY 420

Query: 421 AGLYRYRVGDILRVSGFKNNAPQLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY 480
           AGLYRYRVGDILRVSGFKNNAPQ NFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY
Sbjct: 421 AGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY 480

Query: 481 CACLLEYTSYADTCTIPGHYVIYWEISLKEATAN----IPRIVFEKCCLAMEESLNSVYR 540
            AC++EYTSYADT TIPGHYVIYWEISLKEATAN    IP  VFEKCCL+MEESLNSVYR
Sbjct: 481 GACVVEYTSYADTVTIPGHYVIYWEISLKEATANNNNVIPGSVFEKCCLSMEESLNSVYR 540

Query: 541 QGRVSDKSIGPLEMKIVEPGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVVS 600
           QGRVSDKSIGPLEMKIVE GTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVV 
Sbjct: 541 QGRVSDKSIGPLEMKIVETGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVVD 600

Query: 601 NYFSPKCPNWVPGRKEWSTQRT 618
           NYFSPKCP+WVPGRKEWST  +
Sbjct: 601 NYFSPKCPHWVPGRKEWSTNNS 620

[Close]

BLAST of Sgr023652.1 vs. ExPASy TrEMBL
Match: A0A6J1G8Z2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111451824 PE=3 SV=1)

HSP 1 Score: 1034.2 bits (2673), Expect = 2.1e-298
Identity = 505/595 (84.87%), Postives = 541/595 (90.92%), Query Frame = 0


Query: 23  KDDQA-LQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDRKTFKMTMPLVS 82
           KD+ A LQ+IEE+TTHADQVQRRVLTEILSR+AHVEYLQ+ GL+GC DR  FK  +P+VS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLYSLLM 142
           YE+LKPY+DRIANGDASPILCS P+SEFLTSSGTSGGERKLMPTTEEEL+RRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 123

Query: 143 PVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYNDYTSP 202
           PVMNQF+PGL+KGKGMYFLFVKSESTTPGGL ARPVLTSYY+SSHFKDRPY+ Y DYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDIRTGT 262
           N+TILCTDSYQSMY QFLCGLCLS+ V+RVG+VFASGFIRAIKFLE HW LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVTGTMS 322
           INPRI DPLVRQSVMNILKPDP+LADLIE EC K  WAGII RLWPNTKYIDVIVTGTMS
Sbjct: 244 INPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMS 303

Query: 323 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPLDRTN 382
           QYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC PNEVSYTLIPTMAYFEFLPLDR  
Sbjct: 304 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFSNSACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAP 442
                        K++EELVDLVDVELGKEYELVITTY+GLYRYRVGDILRVSGFKN AP
Sbjct: 364 -------------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAP 423

Query: 443 QLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIPGHYVI 502
           Q NFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY   LLEYTSYADT T+PGHYVI
Sbjct: 424 QFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVI 483

Query: 503 YWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGTFDKLM 562
           YWEIS KE  A IP +VFE CCL +EESLNSVYRQGRVSDKSIGPLE+KI+E GTFDKLM
Sbjct: 484 YWEISQKETNA-IPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLM 543

Query: 563 DYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRKEWSTQR 617
           DYA+NHG+SINQYKTPRCVKH PILELLNSYVVSNYFSP+CP WVPG+KEWST++
Sbjct: 544 DYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTKK 582

[Close]

BLAST of Sgr023652.1 vs. ExPASy TrEMBL
Match: A0A6J1I4E6 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111469778 PE=3 SV=1)

HSP 1 Score: 1030.4 bits (2663), Expect = 3.1e-297
Identity = 504/595 (84.71%), Postives = 541/595 (90.92%), Query Frame = 0


Query: 23  KDDQA-LQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDRKTFKMTMPLVS 82
           KD+ A LQ+IEE+TTHADQVQRRVLTEILSR+AHVEYLQ+ GL+GC DR  FK  +P+VS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLYSLLM 142
           YE+LKPY+DRIANGDASPILCS PISEFLTSSGTSGGERKLMPTT+EEL+RRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPISEFLTSSGTSGGERKLMPTTKEELKRRSFLYSLLM 123

Query: 143 PVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYNDYTSP 202
           PVMNQF+PGL+KGKGMYFLFVKSESTTPGGL ARPVLTSYY+SSHFKDRPY+ Y DYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDIRTGT 262
           N+TILCTDSYQSMYSQFLCGLCLS+ V+RVG+VFASGFIRAIKFLE HW LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVTGTMS 322
           INPRI+DPLVRQSVMNILKPDP+LADLIE EC K  WAGIITRLWPNTKY+DVIVTGTMS
Sbjct: 244 INPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIITRLWPNTKYVDVIVTGTMS 303

Query: 323 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPLDRTN 382
           QYIPTLD YSNGLPLVCTMYASSECYFG+NLNPLC PNEVSYTLIPTMAYFEFLPLDR  
Sbjct: 304 QYIPTLDKYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFSNSACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAP 442
                        K++EELVDLVDVELGKEYEL+ITTY+GLYRYRVGDILRVSGFKN AP
Sbjct: 364 -------------KMSEELVDLVDVELGKEYELIITTYSGLYRYRVGDILRVSGFKNKAP 423

Query: 443 QLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIPGHYVI 502
           Q NFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY   LLEYTSYADT T+PGHYVI
Sbjct: 424 QFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVI 483

Query: 503 YWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGTFDKLM 562
           YWEIS KE  A IP  VFE CCL +EESLNSVYRQGRVSDKSIGPLE+KI+E GTFDKLM
Sbjct: 484 YWEISQKETNA-IPDWVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLM 543

Query: 563 DYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRKEWSTQR 617
           DYA+NHG+SINQYKTPRCVK  PILELLNSYVVSNYFSPKCP WVPG+KEWST++
Sbjct: 544 DYAINHGSSINQYKTPRCVKREPILELLNSYVVSNYFSPKCPKWVPGQKEWSTKK 582

[Close]

BLAST of Sgr023652.1 vs. ExPASy TrEMBL
Match: A0A1U7YQ53 (indole-3-acetic acid-amido synthetase GH3.6 OS=Nelumbo nucifera OX=4432 GN=LOC104585725 PE=3 SV=1)

HSP 1 Score: 963.4 bits (2489), Expect = 4.6e-277
Identity = 462/594 (77.78%), Postives = 522/594 (87.88%), Query Frame = 0


Query: 24  DDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDRKTFKMTMPLVSYE 83
           +  AL++IE+VTT+AD++Q+RVL EILSRSAHVEYLQR+GLNG TDR+TFK  MP+V+YE
Sbjct: 27  NQNALEFIEDVTTNADEIQKRVLAEILSRSAHVEYLQRHGLNGRTDRETFKKVMPVVTYE 86

Query: 84  DLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLYSLLMPV 143
           DL+  ++RIANGD SPILC  PISEFLTSSGTS GERKLMPT EEELERRS LYSLLMPV
Sbjct: 87  DLQSDINRIANGDTSPILCGLPISEFLTSSGTSAGERKLMPTIEEELERRSLLYSLLMPV 146

Query: 144 MNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYNDYTSPNE 203
           M+QFVPGLDKGKGMYFLF+KSE+ TPGGL+ARPVLTSYY+S HFK RPY+ Y +YTSPNE
Sbjct: 147 MDQFVPGLDKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSLHFKHRPYDPYTNYTSPNE 206

Query: 204 TILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDIRTGTIN 263
           TILC DSYQSMYSQ LCGLC ++ V+RVGAVFASGFIRAI+FLE HW+LL NDIRTG IN
Sbjct: 207 TILCPDSYQSMYSQLLCGLCQNKQVLRVGAVFASGFIRAIRFLEKHWSLLCNDIRTGAIN 266

Query: 264 PRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVTGTMSQY 323
           P+ITDP VR +V+ ILKPDP LAD IESEC +E W GIITRLWPNTKYIDVIVTGTMSQY
Sbjct: 267 PQITDPSVRDAVLRILKPDPELADFIESECTREAWQGIITRLWPNTKYIDVIVTGTMSQY 326

Query: 324 IPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPLDRTNGF 383
           IPTLDYYSNGLPLVCTMYASSECYFG+NLNPLCKPNEVSYTLIPTMAYFEFLP+ R NGF
Sbjct: 327 IPTLDYYSNGLPLVCTMYASSECYFGLNLNPLCKPNEVSYTLIPTMAYFEFLPVHRNNGF 386

Query: 384 SNS-ACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQ 443
           + S +     N K  ++LVDLVDV+LG+EYELV+TTYAGLYRYRVGD+LRV+GFKN APQ
Sbjct: 387 TTSISVPKSLNEKEQQDLVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNKAPQ 446

Query: 444 LNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIPGHYVIY 503
            NFVCRKNV+LSIDSDK DEVELQ AV +A  +L+P+ A L+EYTSYADT TIPGHYV+Y
Sbjct: 447 FNFVCRKNVVLSIDSDKTDEVELQAAVKDAINHLVPFDASLIEYTSYADTTTIPGHYVLY 506

Query: 504 WEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGTFDKLMD 563
           WE+   ++T  IP  VFE CCL +EESLNSVYRQGRVSDKSIGPLE+KIVE GTFDKLMD
Sbjct: 507 WELR-HDSTTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKIVETGTFDKLMD 566

Query: 564 YALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRKEWSTQR 617
           YA++ GASINQYKTPRCVK API+ELLNS V+SNYFSPKCP WVPG K+W TQ+
Sbjct: 567 YAISRGASINQYKTPRCVKFAPIVELLNSRVLSNYFSPKCPKWVPGHKQWCTQK 619

[Close]

BLAST of Sgr023652.1 vs. ExPASy TrEMBL
Match: A0A7J7C2G6 (Putative Indole-3-acetic acid-amido synthetase GH3.6 OS=Tripterygium wilfordii OX=458696 GN=HS088_TW21G00496 PE=3 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 1.3e-274
Identity = 455/600 (75.83%), Postives = 524/600 (87.33%), Query Frame = 0


Query: 18  HCSHQKDDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDRKTFKMTM 77
           +C  +K+ + LQ+IE+VT++ D+VQ+RVL EILSR+AHVEYLQR+GLNG TDR+TFK  +
Sbjct: 14  YCLAEKNKKTLQFIEDVTSNPDEVQKRVLEEILSRNAHVEYLQRHGLNGHTDRETFKKLV 73

Query: 78  PLVSYEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLY 137
           P ++YED++  +DRIANGD SPI+CS PISEFLTSSGTSGGERKLMPT EEEL RRS LY
Sbjct: 74  PAITYEDIQSDIDRIANGDTSPIICSKPISEFLTSSGTSGGERKLMPTVEEELGRRSLLY 133

Query: 138 SLLMPVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYND 197
           SLLMPVM+QFVPGL+KGKGMYFLF+KSE+ TPGGL+ARPVLTSYYRSSHFKDRPY+ Y +
Sbjct: 134 SLLMPVMSQFVPGLEKGKGMYFLFIKSEAKTPGGLVARPVLTSYYRSSHFKDRPYDPYTN 193

Query: 198 YTSPNETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDI 257
           YTSPNETILC DSYQSMYSQ LCGLC  + V+RVGAVFASGFIRAI+FLE HW LL NDI
Sbjct: 194 YTSPNETILCPDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIRFLEKHWPLLCNDI 253

Query: 258 RTGTINPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVT 317
           RTGTINP+I+DP VR++VM ILKPDP LAD +E+ C K+ W GIIT+LWPNTKYIDVIVT
Sbjct: 254 RTGTINPQISDPSVREAVMKILKPDPKLADFVEAACGKDSWQGIITKLWPNTKYIDVIVT 313

Query: 318 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPL 377
           GTMSQYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC P+EVSYTLIPTMAYFEFLP+
Sbjct: 314 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCDPSEVSYTLIPTMAYFEFLPV 373

Query: 378 DRTNGFSNS-ACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGF 437
           +R+NG +NS +     N K  +ELVDL +V+LG+EYELV+TTYAGLYRYRVGD+LRV+GF
Sbjct: 374 NRSNGVTNSISMPKTLNEKEQQELVDLAEVKLGQEYELVVTTYAGLYRYRVGDVLRVAGF 433

Query: 438 KNNAPQLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIP 497
           KN APQ NF+CRKNV+LSIDSDK DEVELQNAV  A  NL+P+ A L EYTSYADT TIP
Sbjct: 434 KNRAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNAVNNLVPFDATLAEYTSYADTTTIP 493

Query: 498 GHYVIYWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGT 557
           GHYV++WE++L   T  IP  VFE CCL +EESLNSVYRQGRVSDKSIGPLE+KIVEPGT
Sbjct: 494 GHYVLFWELTLNGLTP-IPPSVFEDCCLTVEESLNSVYRQGRVSDKSIGPLEIKIVEPGT 553

Query: 558 FDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRKEWSTQR 617
           FDKLMDYA++ GASINQYKTPRCVK API+ELLNS  VS+YFSPKCP W PG K+WS ++
Sbjct: 554 FDKLMDYAISLGASINQYKTPRCVKFAPIVELLNSRSVSSYFSPKCPKWAPGHKQWSNKK 612

[Close]

BLAST of Sgr023652.1 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 924.9 bits (2389), Expect = 3.5e-269
Identity = 447/594 (75.25%), Postives = 512/594 (86.20%), Query Frame = 0


Query: 22  QKDDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDRKTFKMTMPLVS 81
           +K+   LQ+IE+VTT+AD VQRRVL EILSR+A VEYL+R+GL G TDR+TFK  MP+V+
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 82  YEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLYSLLM 141
           YED++P ++RIANGD S +LCSNPISEFLTSSGTSGGERKLMPT EEEL+RRS LYSLLM
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 142 PVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYNDYTSP 201
           PVM+QFVPGLDKGKGMYFLF+KSES TPGGL ARPVLTSYY+SSHFK+RPY+ Y +YTSP
Sbjct: 139 PVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSP 198

Query: 202 NETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDIRTGT 261
           N+TILC+DSYQSMYSQ LCGLC  + V+RVGAVFASGFIRAIKFLE HW  LA DIRTGT
Sbjct: 199 NQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGT 258

Query: 262 INPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVTGTMS 321
           ++  ITD  VR++V  ILKPDP LAD +ESECRK  W GIITRLWPNTKY+DVIVTGTMS
Sbjct: 259 LSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMS 318

Query: 322 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPLDRTN 381
           QYIPTLDYYSNGLPLVCTMYASSECYFG+NL PLCKP+EVSYTLIP MAYFEFLP+ R +
Sbjct: 319 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNS 378

Query: 382 GFSNSACESEA-NVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNA 441
           G ++S    +A   K  +ELVDLVDV+LG+EYELV+TTYAGLYRYRVGD+L V+GFKNNA
Sbjct: 379 GVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNA 438

Query: 442 PQLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIPGHYV 501
           PQ +F+CRKNV+LSIDSDK DEVELQNAV  A  +L+P+ A L EYTSYADT +IPGHYV
Sbjct: 439 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 498

Query: 502 IYWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGTFDKL 561
           ++WE+ L   T  IP  VFE CCL +EESLNSVYRQGRVSDKSIGPLE+K+VE GTFDKL
Sbjct: 499 LFWELCLNGNTP-IPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKL 558

Query: 562 MDYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRKEWST 615
           MDYA++ GASINQYKTPRCVK API+ELLNS VV +YFSPKCP W PG K+W +
Sbjct: 559 MDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQWGS 611

[Close]

BLAST of Sgr023652.1 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 919.1 bits (2374), Expect = 1.9e-267
Identity = 447/605 (73.88%), Postives = 516/605 (85.29%), Query Frame = 0


Query: 11  NLQSFDAHCSHQKDDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDR 70
           +L+ FD     QK+ Q LQ IEE+T++ADQVQR+VL EIL+R+A VEYL+R+ LNG TDR
Sbjct: 9   SLEVFDLTLD-QKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDR 68

Query: 71  KTFKMTMPLVSYEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEEL 130
           +TFK  MP+++YED++P ++RIANGD SPIL S PISEFLTSSGTSGGERKLMPT EEEL
Sbjct: 69  ETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEEL 128

Query: 131 ERRSFLYSLLMPVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDR 190
           +RRS LYSLLMPVM+QFVPGL+ GKGMYFLF+KSES TPGGL ARPVLTSYY+SSHFK+R
Sbjct: 129 DRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKER 188

Query: 191 PYNTYNDYTSPNETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHW 250
           PY+ Y +YTSPNETILC+DSYQSMYSQ LCGLC  + V+RVGAVFASGFIRAIKFLE HW
Sbjct: 189 PYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHW 248

Query: 251 ALLANDIRTGTINPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNTK 310
             L  DIRTGT++  ITDP VR++V  ILKP P LAD +E EC+K  W GIITRLWPNTK
Sbjct: 249 IELVRDIRTGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTK 308

Query: 311 YIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMA 370
           Y+DVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFG+NL PLCKP+EVSYTLIP+MA
Sbjct: 309 YVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMA 368

Query: 371 YFEFLPLDRTNGFSNSACESEA-NVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGD 430
           YFEFLP+ R NG +NS    +A   K  +ELVDLVDV+LG+EYELV+TTYAGL RYRVGD
Sbjct: 369 YFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGD 428

Query: 431 ILRVSGFKNNAPQLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSY 490
           +LRV+GFKN APQ +F+CRKNV+LSIDSDK DEVELQNAV  A  +L+P+ A L EYTSY
Sbjct: 429 LLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSY 488

Query: 491 ADTCTIPGHYVIYWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEM 550
           ADT +IPGHYV++WE+ L +    IP  VFE CCLA+EES N+VYRQGRVSDKSIGPLE+
Sbjct: 489 ADTSSIPGHYVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEI 548

Query: 551 KIVEPGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGR 610
           KIVEPGTFDKLMDYA++ GASINQYKTPRCVK API+ELLNS VV +YFSPKCP WVPG 
Sbjct: 549 KIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGH 608

Query: 611 KEWST 615
           K+W +
Sbjct: 609 KQWGS 611

[Close]

BLAST of Sgr023652.1 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 778.1 bits (2008), Expect = 5.3e-225
Identity = 380/602 (63.12%), Postives = 463/602 (76.91%), Query Frame = 0


Query: 10  SNLQSFDAHCSHQKDDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTD 69
           SNL S     + +KD +AL++IEE+T +AD VQ  +L EIL+R+A  EYL+R  L G TD
Sbjct: 5   SNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATD 64

Query: 70  RKTFKMTMPLVSYEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEE 129
           R TFK  +P+++YEDL+P + RIA+GD SPIL ++PISEFLTSSGTS GERKLMPT +EE
Sbjct: 65  RDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEE 124

Query: 130 LERRSFLYSLLMPVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKD 189
           L+RR  LYSLLMPVMN +VPGLDKGKGMYFLFVKSE+ TPGGL ARPVLTSYY+S HF+ 
Sbjct: 125 LDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRS 184

Query: 190 RPYNTYNDYTSPNETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENH 249
           RPY+ YN YTSPNE ILC DS+QSMY+Q LCGL     V+RVGAVFASG +RAI+FL+ H
Sbjct: 185 RPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLH 244

Query: 250 WALLANDIRTGTINPRITDPLVRQSVMNILKPDPMLADLIESECRKEMWAGIITRLWPNT 309
           W+  A+DI  G ++  ITDP +RQ +  ILKPDP+LA+ I  EC+ + W  IITR+WPNT
Sbjct: 245 WSRFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNT 304

Query: 310 KYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTM 369
           KY+DVIVTG M+QYIPTL+YYS GLP+ CTMYASSECYFG+NLNP+ KP+EVSYT++P M
Sbjct: 305 KYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNM 364

Query: 370 AYFEFLPLDRTNGFSNSACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRVGD 429
           AYFEF+PL  T                  + V+LVDV +GKEYELV+TTYAGL RYRVGD
Sbjct: 365 AYFEFIPLGGT------------------KAVELVDVNIGKEYELVVTTYAGLCRYRVGD 424

Query: 430 ILRVSGFKNNAPQLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCAC-LLEYTS 489
           ILRV+GF N+APQ +FV RKNV+LSIDSDK DE ELQ AV  AS  L   C   + EYTS
Sbjct: 425 ILRVTGFHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTS 484

Query: 490 YADTCTIPGHYVIYWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLE 549
           YADT TIPGHYV+YWE+ +++           +CCL MEESLNSVYRQ RV+D S+GPLE
Sbjct: 485 YADTSTIPGHYVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLE 544

Query: 550 MKIVEPGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPG 609
           +++V  GTF++LMDYA++ GASINQYK PRCV   PI+ELL+S VVS +FSP  P+W P 
Sbjct: 545 IRVVRNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPE 588

Query: 610 RK 611
           R+
Sbjct: 605 RR 588

[Close]

BLAST of Sgr023652.1 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 771.5 bits (1991), Expect = 5.0e-223
Identity = 379/592 (64.02%), Postives = 464/592 (78.38%), Query Frame = 0


Query: 22  QKDDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTDRKTFKMTMPLVS 81
           +KD +AL++IEE+T + D VQ +VL EIL+R+++ EYL+R  L+G  DRKTFK  +P+V+
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 82  YEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEELERRSFLYSLLM 141
           YEDLKP + RI+NGD SPIL S+PI+EFLTSSGTS GERKLMPT EE+L+RR  LYSLLM
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 142 PVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKDRPYNTYNDYTSP 201
           PVMN +VPGLDKGKG+YFLFVKSES T GGL ARPVLTSYY+S HFK RPY+ YN YTSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 202 NETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENHWALLANDIRTGT 261
           NE ILC+DS QSMY+Q LCGL +   V+R+GAVFASG +RAI FL+N+W  LA DI TGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 262 INPRITDPLVRQSVMNIL-KPDPMLADLIESECRKEMWAGIITRLWPNTKYIDVIVTGTM 321
           ++ RI DP ++  +  IL KPD  LA+ +   C +E W GIIT++WPNTKY+DVIVTG M
Sbjct: 258 LSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAM 317

Query: 322 SQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIPTMAYFEFLPLDRT 381
           +QYIPTL+YYS GLP+ CTMYASSE YFGINL P+CKP+EVSYT++P MAYFEFLP    
Sbjct: 318 AQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP---- 377

Query: 382 NGFSNSACESEANVKINE-ELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNN 441
               N   +  A   ++E  LV+L +VE+GKEYELVITTYAGLYRYRVGDILRV+GF N+
Sbjct: 378 ---HNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNS 437

Query: 442 APQLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYTSYADTCTIPGHY 501
           APQ  F+ RKNV+LS++SDK DE ELQ AV  AS+        ++EYTSYA+T TIPGHY
Sbjct: 438 APQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHY 497

Query: 502 VIYWE-ISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPLEMKIVEPGTFD 561
           VIYWE +   ++ A +   V  KCCL MEESLNSVYRQ RV+DKSIGPLE+++V  GTF+
Sbjct: 498 VIYWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFE 557

Query: 562 KLMDYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVPGRK 611
           +LMDYA++ GASINQYK PRCV   PI+ELL+S VVS +FSP  P+W P R+
Sbjct: 558 ELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPERR 602

[Close]

BLAST of Sgr023652.1 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 754.2 bits (1946), Expect = 8.2e-218
Identity = 381/603 (63.18%), Postives = 464/603 (76.95%), Query Frame = 0


Query: 10  SNLQSFDAHCSHQKDDQALQYIEEVTTHADQVQRRVLTEILSRSAHVEYLQRNGLNGCTD 69
           S L+S   H    KD +AL++IEE+T + D VQ++V+ EILSR++  EYL+R GL G TD
Sbjct: 5   SALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTD 64

Query: 70  RKTFKMTMPLVSYEDLKPYVDRIANGDASPILCSNPISEFLTSSGTSGGERKLMPTTEEE 129
           RKTFK  +P+V Y+DLKP + RIANGD S IL S PI+EFLTSSGTS GERKLMPT +E+
Sbjct: 65  RKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDED 124

Query: 130 LERRSFLYSLLMPVMNQFVPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSSHFKD 189
           ++RR  LYSLLMPVMN +VPGLDKGK +YFLFVK+ES TPGGL ARPVLTSYY+S  FK 
Sbjct: 125 MDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKR 184

Query: 190 RPYNTYNDYTSPNETILCTDSYQSMYSQFLCGLCLSRGVIRVGAVFASGFIRAIKFLENH 249
           RP + YN YTSPNE ILC DS QSMY+Q LCGL +   V+R+GAVFASG +RAI FL+ +
Sbjct: 185 RPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTN 244

Query: 250 WALLANDIRTGTINPRITDPLVRQSVMNIL-KPDPMLADLIESECRKE-MWAGIITRLWP 309
           W  LA+DI TGT++ RI+DP +++S+  IL KPD  LAD I S C ++  W GIIT++WP
Sbjct: 245 WKELADDISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWP 304

Query: 310 NTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNEVSYTLIP 369
           NTKY+DVIVTG M+QYIP L+YYS GLP+ CTMYASSE YFGINL P+CKP+EVSYT++P
Sbjct: 305 NTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMP 364

Query: 370 TMAYFEFLPLDRTNGFSNSACESEANVKINEELVDLVDVELGKEYELVITTYAGLYRYRV 429
            MAYFEFLP              E   +   ELV+L DVE+GKEYELVITTYAGL RYRV
Sbjct: 365 NMAYFEFLP------------HHEVPTE-KSELVELADVEVGKEYELVITTYAGLNRYRV 424

Query: 430 GDILRVSGFKNNAPQLNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYCACLLEYT 489
           GDIL+V+GF N+APQ  FV RKNV+LSI+SDK DE ELQ+AV  AS  L      ++EYT
Sbjct: 425 GDILQVTGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYT 484

Query: 490 SYADTCTIPGHYVIYWEISLKEATANIPRIVFEKCCLAMEESLNSVYRQGRVSDKSIGPL 549
           SYA+T TIPGHYVIYWE+ +K+ T      V  +CCL MEESLNSVYRQ RV+DKSIGPL
Sbjct: 485 SYAETKTIPGHYVIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPL 544

Query: 550 EMKIVEPGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVVSNYFSPKCPNWVP 609
           E+++V+ GTF++LMDYA++ GASINQYK PRCV   PI+ELL+S VVS +FSP  P+W P
Sbjct: 545 EIRVVKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSP 594

Query: 610 GRK 611
            R+
Sbjct: 605 ERR 594

The following BLAST results are available for this feature:

BLAST of Sgr023652.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140599.1	0.0e+00	86.98	indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 [Momordica charantia...	[more]
XP_022948165.1	4.4e-298	84.87	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
KAG6604833.1	5.7e-298	84.71	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_023533206.1	2.2e-297	84.06	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo]	[more]
XP_022970970.1	6.4e-297	84.71	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima]	[more]

BLAST of Sgr023652.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSQ4	4.9e-268	75.25	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
O81829	2.7e-266	73.88	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	2.1e-226	64.19	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
O82333	7.5e-224	63.12	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=...	[more]
A3BLS0	9.7e-224	63.78	Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399...	[more]

BLAST of Sgr023652.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CG47	0.0e+00	86.98	indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 OS=Momordica charant...	[more]
A0A6J1G8Z2	2.1e-298	84.87	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1I4E6	3.1e-297	84.71	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A1U7YQ53	4.6e-277	77.78	indole-3-acetic acid-amido synthetase GH3.6 OS=Nelumbo nucifera OX=4432 GN=LOC10...	[more]
A0A7J7C2G6	1.3e-274	75.83	Putative Indole-3-acetic acid-amido synthetase GH3.6 OS=Tripterygium wilfordii O...	[more]

BLAST of Sgr023652.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54510.1	3.5e-269	75.25	Auxin-responsive GH3 family protein	[more]
AT4G27260.1	1.9e-267	73.88	Auxin-responsive GH3 family protein	[more]
AT2G14960.1	5.3e-225	63.12	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	5.0e-223	64.02	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	8.2e-218	63.18	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 31..582 e-value: 7.3E-199 score: 662.0
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 16..612
None	No IPR available	PANTHER	PTHR31901:SF63	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE	coord: 16..612

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sgr023652	Sgr023652	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sgr023652.1.exon1	Sgr023652.1.exon1	exon
Sgr023652.1.exon2	Sgr023652.1.exon2	exon
Sgr023652.1.exon3	Sgr023652.1.exon3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Sgr023652.1	cds.Sgr023652.1	CDS
cds.Sgr023652.1	cds.Sgr023652.1_2	CDS
cds.Sgr023652.1	cds.Sgr023652.1_3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sgr023652.1	Sgr023652.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity