Sgr022947.1 (mRNA) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr022947.1
Type	mRNA
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	tocopherol cyclase, chloroplastic
Location	tig00000729: 1276292 .. 1278452 (+)
Sequence length	617
RNA-Seq Expression	Sgr022947.1
Synteny	Sgr022947.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGGAACTCATCAGTTCTCCTCCACTTAATGGAAGCTCGAGCTGGGAAATTCATGTTCGCGGTGAGGATTCCTCAATCTTTGAAGTCCGGCTCATTTCGTCTTCAGTGGGGCGGTTTTTCGAAGCTAGAGTTGCAACCCAAATCGAGCATCCGTGTATTGGGTTCCAGCTCGAACTCTGTGTATGAATCTTCTGCGTCGTCGAAAGTTGAGAAAGATGACGAAGGTCTTGTTTGCCCTCCTGGGTATCGGCCCACGCCTGCTAATCGCGAGCTGCGGACTCCTCACAGCGGGTAATCTTTGCCAAGTCAAATCTTGTCTGGAGTTAAGTTCTTACAAAATTTTTGCATCTCTAGTATGAATTGTTCATGCAATGTTTGACTCGGCCAGTTACAATTTTAGATAATTTCTGGAGCATTTGCACCCGGTATAACCTTATTTTTGTTATGTGATCCCAGTTGAATAGTTATTCATATTTAGAAGATAGCTGCATCAAGCAATTGGTTGATCTTGTTATATCTGGTTCTGTGGCAATCACTGTTTTATGTTCCCCATATTTTTCGTACTTTGTACTTGAATCAGGTATCACTTTGATGGGAGTACTCGGAAATTCTTTGAAGGTTGGTACTTGAAGGTATCAATTCCACAGAAGAGGCAGAGTTTCTGCTTCATGTATTCGGTTGAGAACCCTGCATTTCCTAAGAAGTTAACAACACTAGAAATCGCACAAAATGGACCTAGATATACAGGAGTTGGAGCACAGATTCTTGGTGCTTATGACAAATACATATGCCAATACTCCGAAGAGTCTCCAAACTTTTGGGGAAGTAAGTTTTTATACGAAACGTATCTTCTTTTCTCAGTTCTTCTTTACAATGCTTCCTTAGTGAGATCAATTTTTTAACTTGAGGTCTTGTTTTATGACAAGTCTATGAACTGTTCAAAACTTCCATATATTGGCTGTTCTATCAATGCCTTGCGTTTTGGATCAACTGCTGCTAACTTTATGTTCAAATATTATTTGATACACAAGAAATTATAGAACGACCTTCCCCACTCTCCAGACCAAACATATATTCACTGCCAGTTGGAATTTTAAATGGATTTTTTCCCCTACACCTTTCTTGATTGTCTTGTAATGTGCATAATGAAATACATAAGCAACTTTCATTGTCAGGTAGTTGCTAATTTTGCTCCATAATTTGAAAATTTTGGATTTTGAACCAAAATTTTATTTAAACTGAAGTCAGAAAGTTTGCCATCAACCTTTTTTGATTTATTTAGATCCTAAGGTGGTTTCCACAAGCGACCTAGTCTAATTAAGAATGCATTTTAGCAAAAACACATTTAACTACTGTGTATGACTTTGTCCTTTCAGTTTTTCCAGCATGCATATTTGTTTTCGGAATGATATTTGGTTATCGTGGCCTATTCTCCCTCCAATCAATTTGGCCTTTGTCCTCTTGGTCGTCCATCTCAACTGCAAGAGAAATACTCCTGAGAGGAAATTGGATGAGTTGGTTATTGATAGGCTGAACCTCCCTTATGCAAGGAGGGGCACACGGGGGAAAAGCACACAAGGAAGGGTGAGCTGAGTTAGTTATTGTATAGAGGGAATATTTTGTTAGGGGGACCAGCAGGTCCTATTCAGTCAGGATGGTACATGTTAGTAGTGAGGGAGTGTAGGAAGGTATGTGAATATTGTGTAATGTTGGGAGAGATTCAGACCTCTCGAAAAAGAGTCTGTCCTTGTGTTCTGTTTTGTTATTTCCTTTTTGACCATCTAATAGTATATCTCAGTAATATTGCTCCCCAATTGTTTTATCAGTTTCTTTGCTAGGTATCCTTTAGATGATTGCTATCTGTCGTAATTAAAGGAATTTAAGTGTATAAGCGGAGAATTTGATTCTTGTCATAATGAAGAGAAAGATCATTTATCCTGTTTGTCTGGCACTTCATATCCCTTGATTCTCAGAATATATTTCTAAAGAGTTCTCGACCTCTTTATATAGGCTATGATTTTTAGATGTCTTATTTTTTCTAAAAAAAATGGGGGACTAATACTTGGTTTTTAATGCATAGGTAGGCATGAACTAAGCCTGGGCAACAGTTTTGCAACTAAAGGAGGGTTAAGCCTCCAAACAAGGAAGTTCCTCCGGAG

mRNA sequence

ATGCGGAACTCATCAGTTCTCCTCCACTTAATGGAAGCTCGAGCTGGGAAATTCATGTTCGCGGTGAGGATTCCTCAATCTTTGAAGTCCGGCTCATTTCGTCTTCAGTGGGGCGGTTTTTCGAAGCTAGAGTTGCAACCCAAATCGAGCATCCGTGTATTGGGTTCCAGCTCGAACTCTGTGTATGAATCTTCTGCGTCGTCGAAAGTTGAGAAAGATGACGAAGGTCTTGTTTGCCCTCCTGGGTATCGGCCCACGCCTGCTAATCGCGAGCTGCGGACTCCTCACAGCGGGTATCACTTTGATGGGAGTACTCGGAAATTCTTTGAAGGTTGGTACTTGAAGGTATCAATTCCACAGAAGAGGCAGAGTTTCTGCTTCATGTATTCGGTTGAGAACCCTGCATTTCCTAAGAAGTTAACAACACTAGAAATCGCACAAAATGGACCTAGATATACAGGAGTTGGAGCACAGATTCTTGGTGCTTATGACAAATACATATGCCAATACTCCGAAGAGTCTCCAAACTTTTGGGGAAGTAGGCATGAACTAAGCCTGGGCAACAGTTTTGCAACTAAAGGAGGGTTAAGCCTCCAAACAAGGAAGTTCCTCCGGAG

Coding sequence (CDS)

ATGCGGAACTCATCAGTTCTCCTCCACTTAATGGAAGCTCGAGCTGGGAAATTCATGTTCGCGGTGAGGATTCCTCAATCTTTGAAGTCCGGCTCATTTCGTCTTCAGTGGGGCGGTTTTTCGAAGCTAGAGTTGCAACCCAAATCGAGCATCCGTGTATTGGGTTCCAGCTCGAACTCTGTGTATGAATCTTCTGCGTCGTCGAAAGTTGAGAAAGATGACGAAGGTCTTGTTTGCCCTCCTGGGTATCGGCCCACGCCTGCTAATCGCGAGCTGCGGACTCCTCACAGCGGGTATCACTTTGATGGGAGTACTCGGAAATTCTTTGAAGGTTGGTACTTGAAGGTATCAATTCCACAGAAGAGGCAGAGTTTCTGCTTCATGTATTCGGTTGAGAACCCTGCATTTCCTAAGAAGTTAACAACACTAGAAATCGCACAAAATGGACCTAGATATACAGGAGTTGGAGCACAGATTCTTGGTGCTTATGACAAATACATATGCCAATACTCCGAAGAGTCTCCAAACTTTTGGGGAAGTAGGCATGAACTAAGCCTGGGCAACAGTTTTGCAACTAAAGGAGGGTTAAGCCTCCAAACAAGGAAGTTCCTCCGGAG

Protein sequence

MRNSSVLLHLMEARAGKFMFAVRIPQSLKSGSFRLQWGGFSKLELQPKSSIRVLGSSSNSVYESSASSKVEKDDEGLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYLKVSIPQKRQSFCFMYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESPNFWGSRHELSLGNSFATKGGLSLQTRKFLRX

Homology

[Close]

BLAST of Sgr022947.1 vs. NCBI nr
Match: XP_022131899.1 (tocopherol cyclase, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 344.4 bits (882), Expect = 7.0e-91
Identity = 167/188 (88.83%), Postives = 175/188 (93.09%), Query Frame = 0


Query: 9   HLMEARAGKFMFAVRIPQSLKSGSFRLQWGGFSKLELQPKSSIRVLGSSSNSVYESSASS 68
           +LME +AGKFMF V++PQSL+S SFRLQWG  SKLE + +SSIRVL SSSNSVYESS SS
Sbjct: 19  NLMETQAGKFMFEVKLPQSLRSRSFRLQWGRSSKLESRSRSSIRVLASSSNSVYESSVSS 78

Query: 69  KVEK-DDEGLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYLKVSIPQKRQSFCF 128
           K+EK DD GLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWY KVSIPQKRQSFCF
Sbjct: 79  KIEKEDDGGLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYFKVSIPQKRQSFCF 138

Query: 129 MYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESPNFWGSRHELSLG 188
           MYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEES NFWGSRHELSLG
Sbjct: 139 MYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESQNFWGSRHELSLG 198

Query: 189 NSFATKGG 196
           NSFATKGG
Sbjct: 199 NSFATKGG 206

[Close]

BLAST of Sgr022947.1 vs. NCBI nr
Match: XP_022131896.1 (tocopherol cyclase, chloroplastic isoform X1 [Momordica charantia] >XP_022131897.1 tocopherol cyclase, chloroplastic isoform X1 [Momordica charantia] >XP_022131898.1 tocopherol cyclase, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 344.4 bits (882), Expect = 7.0e-91
Identity = 167/188 (88.83%), Postives = 175/188 (93.09%), Query Frame = 0


Query: 9   HLMEARAGKFMFAVRIPQSLKSGSFRLQWGGFSKLELQPKSSIRVLGSSSNSVYESSASS 68
           +LME +AGKFMF V++PQSL+S SFRLQWG  SKLE + +SSIRVL SSSNSVYESS SS
Sbjct: 19  NLMETQAGKFMFEVKLPQSLRSRSFRLQWGRSSKLESRSRSSIRVLASSSNSVYESSVSS 78

Query: 69  KVEK-DDEGLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYLKVSIPQKRQSFCF 128
           K+EK DD GLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWY KVSIPQKRQSFCF
Sbjct: 79  KIEKEDDGGLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYFKVSIPQKRQSFCF 138

Query: 129 MYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESPNFWGSRHELSLG 188
           MYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEES NFWGSRHELSLG
Sbjct: 139 MYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESQNFWGSRHELSLG 198

Query: 189 NSFATKGG 196
           NSFATKGG
Sbjct: 199 NSFATKGG 206

[Close]

BLAST of Sgr022947.1 vs. NCBI nr
Match: XP_023538605.1 (tocopherol cyclase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 331.3 bits (848), Expect = 6.1e-87
Identity = 159/186 (85.48%), Postives = 172/186 (92.47%), Query Frame = 0


Query: 11  MEARAGKFMFAVRIPQSLKSGSFRLQWGGFSKLELQPKSSIRVLGSSSNSVYESSASSKV 70
           ME +AGK MFAV++PQSLKS SFR +WGG SKL+L+ +SSIRVL SSSNSVYESSAS+K+
Sbjct: 21  MEVQAGKSMFAVKVPQSLKSSSFRRRWGGSSKLKLKSRSSIRVLASSSNSVYESSASAKI 80

Query: 71  EKD-DEGLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYLKVSIPQKRQSFCFMY 130
           EKD D GLVCPPGY+PTPANRELRTPHSGYHFDGSTRKFFEGWY KVSIPQKRQSFCFMY
Sbjct: 81  EKDNDGGLVCPPGYQPTPANRELRTPHSGYHFDGSTRKFFEGWYFKVSIPQKRQSFCFMY 140

Query: 131 SVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESPNFWGSRHELSLGNS 190
           SVENPAFPKKLTT EI Q+GPR+TGVGAQILGAYDKYICQYSEES NFWGSRHELSLGN 
Sbjct: 141 SVENPAFPKKLTTPEIVQHGPRFTGVGAQILGAYDKYICQYSEESQNFWGSRHELSLGNC 200

Query: 191 FATKGG 196
           FA+KGG
Sbjct: 201 FASKGG 206

[Close]

BLAST of Sgr022947.1 vs. NCBI nr
Match: XP_038884981.1 (tocopherol cyclase, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 330.5 bits (846), Expect = 1.0e-86
Identity = 158/187 (84.49%), Postives = 171/187 (91.44%), Query Frame = 0


Query: 10  LMEARAGKFMFAVRIPQSLKSGSFRLQWGGFSKLELQPKSSIRVLGSSSNSVYESSASSK 69
           LMEA+AGK MF V +PQSLKS SF L+WGG SKL  + +SSIRVL SSSNSVYESSA+SK
Sbjct: 20  LMEAQAGKSMFGVELPQSLKSSSFHLRWGGSSKLRQKSRSSIRVLASSSNSVYESSATSK 79

Query: 70  VEKDDEG-LVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYLKVSIPQKRQSFCFM 129
           +EKDD+G LVCPPGY+PTP NRELRTPHSGYHFDGSTRKFFEGWY KVSIPQKRQSFCFM
Sbjct: 80  IEKDDDGSLVCPPGYQPTPGNRELRTPHSGYHFDGSTRKFFEGWYFKVSIPQKRQSFCFM 139

Query: 130 YSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESPNFWGSRHELSLGN 189
           YSVENPAFPKKLT +EIAQ+GPR+TGVGAQILGAYDKYICQYSE+S NFWGSRHEL LGN
Sbjct: 140 YSVENPAFPKKLTPVEIAQHGPRFTGVGAQILGAYDKYICQYSEDSQNFWGSRHELCLGN 199

Query: 190 SFATKGG 196
           SFATKGG
Sbjct: 200 SFATKGG 206

[Close]

BLAST of Sgr022947.1 vs. NCBI nr
Match: XP_038884980.1 (tocopherol cyclase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 330.5 bits (846), Expect = 1.0e-86
Identity = 158/187 (84.49%), Postives = 171/187 (91.44%), Query Frame = 0


Query: 10  LMEARAGKFMFAVRIPQSLKSGSFRLQWGGFSKLELQPKSSIRVLGSSSNSVYESSASSK 69
           LMEA+AGK MF V +PQSLKS SF L+WGG SKL  + +SSIRVL SSSNSVYESSA+SK
Sbjct: 20  LMEAQAGKSMFGVELPQSLKSSSFHLRWGGSSKLRQKSRSSIRVLASSSNSVYESSATSK 79

Query: 70  VEKDDEG-LVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYLKVSIPQKRQSFCFM 129
           +EKDD+G LVCPPGY+PTP NRELRTPHSGYHFDGSTRKFFEGWY KVSIPQKRQSFCFM
Sbjct: 80  IEKDDDGSLVCPPGYQPTPGNRELRTPHSGYHFDGSTRKFFEGWYFKVSIPQKRQSFCFM 139

Query: 130 YSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESPNFWGSRHELSLGN 189
           YSVENPAFPKKLT +EIAQ+GPR+TGVGAQILGAYDKYICQYSE+S NFWGSRHEL LGN
Sbjct: 140 YSVENPAFPKKLTPVEIAQHGPRFTGVGAQILGAYDKYICQYSEDSQNFWGSRHELCLGN 199

Query: 190 SFATKGG 196
           SFATKGG
Sbjct: 200 SFATKGG 206

[Close]

BLAST of Sgr022947.1 vs. ExPASy Swiss-Prot
Match: Q94FY7 (Tocopherol cyclase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=VTE1 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 4.9e-47
Identity = 91/156 (58.33%), Postives = 111/156 (71.15%), Query Frame = 0


Query: 40  FSKLELQPKSSIRVLGSSSNSVYESSASSKVEKDDEGLVCPPGYRPTPANRELRTPHSGY 99
           F   +L P+S  RV          S++ S    + + +   P Y PT  NRELRTPHSGY
Sbjct: 33  FRSTKLVPRSISRV----------SASISTPNSETDKISVKPVYVPTSPNRELRTPHSGY 92

Query: 100 HFDGSTRKFFEGWYLKVSIPQKRQSFCFMYSVENPAFPKKLTTLEIAQNGPRYTGVGAQI 159
           HFDG+ RKFFEGWY +VSIP+KR+SFCFMYSVENPAF + L+ LE+A  GPR+TGVGAQI
Sbjct: 93  HFDGTPRKFFEGWYFRVSIPEKRESFCFMYSVENPAFRQSLSPLEVALYGPRFTGVGAQI 152

Query: 160 LGAYDKYICQYSEESPNFWGSRHELSLGNSFATKGG 196
           LGA DKY+CQY ++S NFWG RHEL LGN+F+   G
Sbjct: 153 LGANDKYLCQYEQDSHNFWGDRHELVLGNTFSAVPG 178

[Close]

BLAST of Sgr022947.1 vs. ExPASy Swiss-Prot
Match: Q6K7V6 (Probable tocopherol cyclase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=VTE1 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 9.6e-43
Identity = 79/118 (66.95%), Postives = 92/118 (77.97%), Query Frame = 0


Query: 78  VCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYLKVSIPQKRQSFCFMYSVENPAFP 137
           V  P Y PTP +R LRTPHSGYH+DG+ R FFEGWY KVSIP+ RQSFCFMYSVENP F 
Sbjct: 46  VAAPVYAPTPRDRALRTPHSGYHYDGTARPFFEGWYFKVSIPECRQSFCFMYSVENPLFR 105

Query: 138 KKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESPNFWGSRHELSLGNSFATKGG 196
             ++ L+   +G R+TGVGAQILGA DKYICQ++E+S NFWGSRHEL LGN+F    G
Sbjct: 106 DGMSDLDRVIHGSRFTGVGAQILGADDKYICQFTEKSNNFWGSRHELMLGNTFIPNNG 163

[Close]

BLAST of Sgr022947.1 vs. ExPASy Swiss-Prot
Match: Q94FY8 (Tocopherol cyclase, chloroplastic OS=Zea mays OX=4577 GN=SXD1 PE=1 SV=2)

HSP 1 Score: 172.9 bits (437), Expect = 3.7e-42
Identity = 88/145 (60.69%), Postives = 100/145 (68.97%), Query Frame = 0


Query: 47  PKSSIRVLGSSSNSVYESSASSKVEK-DDEGLVCPPGYRPTPANRELRTPHSGYHFDGST 106
           P+SS R        V   +ASS V           P Y PTP +R LRTPHSGYHFDG+ 
Sbjct: 21  PRSSRR---HCPRGVVPRAASSSVSSFTSPSAAAAPIYTPTPQDRSLRTPHSGYHFDGTA 80

Query: 107 RKFFEGWYLKVSIPQKRQSFCFMYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDK 166
           R FFEGWY KVSIP+ RQSFCFMYSVENP F   ++ L+     PR+TGVGAQILGA DK
Sbjct: 81  RPFFEGWYFKVSIPECRQSFCFMYSVENPLFRDGMSDLDKLLYRPRFTGVGAQILGADDK 140

Query: 167 YICQYSEESPNFWGSRHELSLGNSF 191
           YICQ+SE+S NFWGSRHEL LGN+F
Sbjct: 141 YICQFSEKSNNFWGSRHELMLGNTF 162

[Close]

BLAST of Sgr022947.1 vs. ExPASy TrEMBL
Match: A0A6J1BUS2 (tocopherol cyclase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004926 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.4e-91
Identity = 167/188 (88.83%), Postives = 175/188 (93.09%), Query Frame = 0


Query: 9   HLMEARAGKFMFAVRIPQSLKSGSFRLQWGGFSKLELQPKSSIRVLGSSSNSVYESSASS 68
           +LME +AGKFMF V++PQSL+S SFRLQWG  SKLE + +SSIRVL SSSNSVYESS SS
Sbjct: 19  NLMETQAGKFMFEVKLPQSLRSRSFRLQWGRSSKLESRSRSSIRVLASSSNSVYESSVSS 78

Query: 69  KVEK-DDEGLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYLKVSIPQKRQSFCF 128
           K+EK DD GLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWY KVSIPQKRQSFCF
Sbjct: 79  KIEKEDDGGLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYFKVSIPQKRQSFCF 138

Query: 129 MYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESPNFWGSRHELSLG 188
           MYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEES NFWGSRHELSLG
Sbjct: 139 MYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESQNFWGSRHELSLG 198

Query: 189 NSFATKGG 196
           NSFATKGG
Sbjct: 199 NSFATKGG 206

[Close]

BLAST of Sgr022947.1 vs. ExPASy TrEMBL
Match: A0A6J1BRJ2 (tocopherol cyclase, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004926 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.4e-91
Identity = 167/188 (88.83%), Postives = 175/188 (93.09%), Query Frame = 0


Query: 9   HLMEARAGKFMFAVRIPQSLKSGSFRLQWGGFSKLELQPKSSIRVLGSSSNSVYESSASS 68
           +LME +AGKFMF V++PQSL+S SFRLQWG  SKLE + +SSIRVL SSSNSVYESS SS
Sbjct: 19  NLMETQAGKFMFEVKLPQSLRSRSFRLQWGRSSKLESRSRSSIRVLASSSNSVYESSVSS 78

Query: 69  KVEK-DDEGLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYLKVSIPQKRQSFCF 128
           K+EK DD GLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWY KVSIPQKRQSFCF
Sbjct: 79  KIEKEDDGGLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYFKVSIPQKRQSFCF 138

Query: 129 MYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESPNFWGSRHELSLG 188
           MYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEES NFWGSRHELSLG
Sbjct: 139 MYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESQNFWGSRHELSLG 198

Query: 189 NSFATKGG 196
           NSFATKGG
Sbjct: 199 NSFATKGG 206

[Close]

BLAST of Sgr022947.1 vs. ExPASy TrEMBL
Match: A0A6J1KNK8 (tocopherol cyclase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111496186 PE=4 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.1e-86
Identity = 157/186 (84.41%), Postives = 171/186 (91.94%), Query Frame = 0


Query: 11  MEARAGKFMFAVRIPQSLKSGSFRLQWGGFSKLELQPKSSIRVLGSSSNSVYESSASSKV 70
           ME +AGK MFAV++PQSLKS SFRL WGG SKL+L+ +SS+ VL SSSNSVYESSAS+K+
Sbjct: 21  MEVQAGKSMFAVKVPQSLKSSSFRLPWGGSSKLKLKSRSSVLVLASSSNSVYESSASAKI 80

Query: 71  EKDDE-GLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYLKVSIPQKRQSFCFMY 130
           E DD+ GLVCPPGY+PTPANRELRTPHSGYHFDGSTRKFFEGWY KVSIPQKRQSFCFMY
Sbjct: 81  ENDDDGGLVCPPGYQPTPANRELRTPHSGYHFDGSTRKFFEGWYFKVSIPQKRQSFCFMY 140

Query: 131 SVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESPNFWGSRHELSLGNS 190
           SVENPAFPKKLTT EI Q+GPR+TGVGAQILGAYDKYICQYSEES NFWGSRHELSLGN 
Sbjct: 141 SVENPAFPKKLTTPEIVQHGPRFTGVGAQILGAYDKYICQYSEESQNFWGSRHELSLGNC 200

Query: 191 FATKGG 196
           FA+KGG
Sbjct: 201 FASKGG 206

[Close]

BLAST of Sgr022947.1 vs. ExPASy TrEMBL
Match: A0A6J1GIN5 (tocopherol cyclase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111454510 PE=4 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 1.9e-86
Identity = 157/186 (84.41%), Postives = 171/186 (91.94%), Query Frame = 0


Query: 11  MEARAGKFMFAVRIPQSLKSGSFRLQWGGFSKLELQPKSSIRVLGSSSNSVYESSASSKV 70
           ME +AGK MFAV++PQSLKS SFR +WGG SKL+L+ +S IRVL SSSNSVYESSAS+K+
Sbjct: 21  MEVQAGKSMFAVKVPQSLKSSSFRHRWGGSSKLKLKSRSRIRVLASSSNSVYESSASAKI 80

Query: 71  EKDDE-GLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYLKVSIPQKRQSFCFMY 130
           E DD+ GLVCPPGY+PTPANRELRTPHSGYHFDGSTRKFFEGWY KVSIPQKRQSFCFMY
Sbjct: 81  ENDDDGGLVCPPGYQPTPANRELRTPHSGYHFDGSTRKFFEGWYFKVSIPQKRQSFCFMY 140

Query: 131 SVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESPNFWGSRHELSLGNS 190
           SVENPAFPKKLTT EI Q+GPR+TGVGAQILGAYDKYICQYSEES NFWGSRHELSLGN 
Sbjct: 141 SVENPAFPKKLTTPEIVQHGPRFTGVGAQILGAYDKYICQYSEESQNFWGSRHELSLGNC 200

Query: 191 FATKGG 196
           FA+KGG
Sbjct: 201 FASKGG 206

[Close]

BLAST of Sgr022947.1 vs. ExPASy TrEMBL
Match: A0A5A7VA31 (Tocopherol cyclase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003600 PE=4 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.6e-85
Identity = 161/189 (85.19%), Postives = 174/189 (92.06%), Query Frame = 0


Query: 10  LMEARAGKFMFAVRIPQSLK-SGSFRLQWGGFSKLELQPKS-SIRVLGSSSNSVYESSAS 69
           L+EA+AG+ MF V +P SLK S SFRL+WGG SKLEL+ +S SIRVL SSSNSVYESSAS
Sbjct: 20  LVEAQAGRPMFGVELPLSLKSSSSFRLRWGGSSKLELKSRSTSIRVLASSSNSVYESSAS 79

Query: 70  SKVEKDDE-GLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYLKVSIPQKRQSFC 129
            K+EKDD+ GLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWY KVSIPQKRQSFC
Sbjct: 80  LKIEKDDDGGLVCPPGYRPTPANRELRTPHSGYHFDGSTRKFFEGWYFKVSIPQKRQSFC 139

Query: 130 FMYSVENPAFPKKLTTLEIAQNGPRYTGVGAQILGAYDKYICQYSEESPNFWGSRHELSL 189
           FMYSVENPAFPKKLT ++IAQ+GPR+TGVGAQILGAYDKYICQYSEES NFWGSRHELSL
Sbjct: 140 FMYSVENPAFPKKLTAVDIAQHGPRFTGVGAQILGAYDKYICQYSEESQNFWGSRHELSL 199

Query: 190 GNSFATKGG 196
           GNSFATKGG
Sbjct: 200 GNSFATKGG 208

[Close]

BLAST of Sgr022947.1 vs. TAIR 10
Match: AT4G32770.1 (tocopherol cyclase, chloroplast / vitamin E deficient 1 (VTE1) / sucrose export defective 1 (SXD1) )

HSP 1 Score: 189.1 bits (479), Expect = 3.5e-48
Identity = 91/156 (58.33%), Postives = 111/156 (71.15%), Query Frame = 0


Query: 40  FSKLELQPKSSIRVLGSSSNSVYESSASSKVEKDDEGLVCPPGYRPTPANRELRTPHSGY 99
           F   +L P+S  RV          S++ S    + + +   P Y PT  NRELRTPHSGY
Sbjct: 33  FRSTKLVPRSISRV----------SASISTPNSETDKISVKPVYVPTSPNRELRTPHSGY 92

Query: 100 HFDGSTRKFFEGWYLKVSIPQKRQSFCFMYSVENPAFPKKLTTLEIAQNGPRYTGVGAQI 159
           HFDG+ RKFFEGWY +VSIP+KR+SFCFMYSVENPAF + L+ LE+A  GPR+TGVGAQI
Sbjct: 93  HFDGTPRKFFEGWYFRVSIPEKRESFCFMYSVENPAFRQSLSPLEVALYGPRFTGVGAQI 152

Query: 160 LGAYDKYICQYSEESPNFWGSRHELSLGNSFATKGG 196
           LGA DKY+CQY ++S NFWG RHEL LGN+F+   G
Sbjct: 153 LGANDKYLCQYEQDSHNFWGDRHELVLGNTFSAVPG 178

The following BLAST results are available for this feature:

BLAST of Sgr022947.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131899.1	7.0e-91	88.83	tocopherol cyclase, chloroplastic isoform X2 [Momordica charantia]	[more]
XP_022131896.1	7.0e-91	88.83	tocopherol cyclase, chloroplastic isoform X1 [Momordica charantia] >XP_022131897...	[more]
XP_023538605.1	6.1e-87	85.48	tocopherol cyclase, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_038884981.1	1.0e-86	84.49	tocopherol cyclase, chloroplastic isoform X2 [Benincasa hispida]	[more]
XP_038884980.1	1.0e-86	84.49	tocopherol cyclase, chloroplastic isoform X1 [Benincasa hispida]	[more]

BLAST of Sgr022947.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94FY7	4.9e-47	58.33	Tocopherol cyclase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=VTE1 PE=1 S...	[more]
Q6K7V6	9.6e-43	66.95	Probable tocopherol cyclase, chloroplastic OS=Oryza sativa subsp. japonica OX=39...	[more]
Q94FY8	3.7e-42	60.69	Tocopherol cyclase, chloroplastic OS=Zea mays OX=4577 GN=SXD1 PE=1 SV=2	[more]

BLAST of Sgr022947.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BUS2	3.4e-91	88.83	tocopherol cyclase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1BRJ2	3.4e-91	88.83	tocopherol cyclase, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1KNK8	1.1e-86	84.41	tocopherol cyclase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111496186 PE...	[more]
A0A6J1GIN5	1.9e-86	84.41	tocopherol cyclase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111454510 ...	[more]
A0A5A7VA31	1.6e-85	85.19	Tocopherol cyclase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00...	[more]

BLAST of Sgr022947.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32770.1	3.5e-48	58.33	tocopherol cyclase, chloroplast / vitamin E deficient 1 (VTE1) / sucrose export ...	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025893	Tocopherol cyclase	PFAM	PF14249	Tocopherol_cycl	coord: 106..195 e-value: 6.2E-25 score: 88.0
IPR025893	Tocopherol cyclase	PANTHER	PTHR35309	FAMILY NOT NAMED	coord: 38..194
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 65..90

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sgr022947	Sgr022947	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sgr022947.1.exon1	Sgr022947.1.exon1	exon
Sgr022947.1.exon2	Sgr022947.1.exon2	exon
Sgr022947.1.exon3	Sgr022947.1.exon3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Sgr022947.1	cds.Sgr022947.1	CDS
cds.Sgr022947.1	cds.Sgr022947.1_2	CDS
cds.Sgr022947.1	cds.Sgr022947.1_3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sgr022947.1	Sgr022947.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015994	chlorophyll metabolic process
biological_process	GO:0006631	fatty acid metabolic process
biological_process	GO:0009915	phloem sucrose loading
biological_process	GO:0031347	regulation of defense response
biological_process	GO:0009644	response to high light intensity
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0009266	response to temperature stimulus
biological_process	GO:0010189	vitamin E biosynthetic process
biological_process	GO:0016122	xanthophyll metabolic process
cellular_component	GO:0010287	plastoglobule
molecular_function	GO:0009976	tocopherol cyclase activity