Sgr018216.1 (mRNA) Monk fruit (Qingpiguo) v1

Overview
NameSgr018216.1
TypemRNA
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionmediator of RNA polymerase II transcription subunit 27
Locationtig00153145: 274995 .. 278059 (+)
Sequence length1272
RNA-Seq ExpressionSgr018216.1
SyntenySgr018216.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGCCAGACGCAGCAGCCAGCGCAGGGCCCGACTGCGACGGTGAGCCCTAATACTGCTGCGCCTCATTCCGGCGGCTCTGCAGACGAGGCGCCGCCGAAGCAGGTAGCGCTGGCCATGGATAGACTCGGCCACGCCGCACGCCTCATCGCCGATATTAGGCTCGGCGCCGATCGTCTTCTCGAAGCCTTATGCGTCACTGCACAGCCTCATCAAAGCACCAAGCCTCTGCACTTGTTTCAGAAAGAGGATGCTTCGATGCGGCAACACCTCCAAGACCTTCGTGCAGTTGGTATTTAGCATCAATTCTCTTAGGGTTGCGTTTTCAATTTGAATGCTTAGGGTAAAATCTTATCATTTTAAGACCACAACTAGACAATTTTAGTATCAAATTGTCCATATTGAAGAGGGGGTTTAATTTTTCTGGCAATTTGGATGAGATCATGCAATTGAAATAGGGTGTAACATTAGCAAAACTACAAATATTTTTCTTCTAATTTTGTTAGGAAGGCAGCTTGAAGAGTCTGGCGTTCTCAGTGAATCTCTTCTTTCACGAAGTAATTCTTGGGGTCTACATATGCCTTTAGTGTGCCCTGACGGAGCAGTGGTTGCTTATGCTTGGAAACGTCAACTAGCTGGCCAGGCCGGTGCATCCGCAGTTGACCGGACCAGGTTGGTTAAGACATTTTGCATATACCCATCCACTAAAATAGCTGCTCTATGGCATAAATACGGAAACCTACTTGAATTTGCAAGTTCACTTGATTTTTCTTCATATTGCTTAAAGCAGTAGCGTGGTAGCATCGATTGCTAAATTAGAGTTCATAAATTTATTTTTTAAAGAAACAGTAGGGATTCCAGGCTTGGCTGATGATGCCAATAAGTTGTGGTTGATATTTAAGGAAGTTCAAGGCTGTAACAGACTGGATAGTGAATTGCAGCAACTTGACTTTGCAGCATTGATATTTAAGGAAATTCTAATAATTCCATCAAGTTTCTTACATACATGTATATAAGATAATTCAATGGTCAATAATAAGAACATGTGTTGTAAGATGGATAGATACCTTGTGGATATGATATTTCATTGTTAATTAATTACCATTAGGCTATGGGCATTGTCGAACAATATAGAAAATTTTTATTTTTTATGATCATTCTCGCGTTGTAACCAGAAAGTAAAGGACCCTGGATTTAATGAAGTGATCATAGTGTTACATTGCTTTTGTCACCTACACAGGGTTTTAATTATTTAACAAATTTTCTAATTCAGAGAGCTGAAGCTTACTAATGATTCACTAATGAATATATTTGTGCTTCTATTATACTGTAGGACAGCTTCAGTTGCCATTGATTGTTATGTCTTTTTGAATTATGACCATGTAGTTGCCTCCTTATATATGATAGACCAGGTCTTGTAAATTTTAATCTCAATTCCATAAGTTCAGGTAGATGTTTTACTGATGCTTGAACATGATGATAGGTTAGCTCTAAAGGCCTTCACTGACCAGAAAAGACGGTTTTTCCCTCATCTTGACGATGAAAATGGGTCGAATATTGAGCCTGTTCTGAAGAAGCCTTGTTTTTCTCATGGAGTGACAGAAATTAAACAAGAGGAGCTTTATGAATCTAGAACACTGCCAGATATTCTAACATGTATGGAAAAGGAAGTGCCAGATCTTAAGATTTTCACTTACGAGCGATTAGACTGGTTGAAACAAGCTTCAGCACTTCCCTCACAAGCAAATGAGAATTCCATGGGAGCATTGAAAGAACATAACTATCATAGTCCTAGGATGAGCCCTGGATCGCTTGGTGCTACTGCCAAAGATAAAGTTGCTGTAATTGAGTTGATCATCCCTTCTGTCTTTAGAGCTGTAGTATCACTGCATCCAGTTGGTTCTGTTGAACCAGATGCTGTAGCTTTCTTTTCTCCTGATGAGGTACATTATTTTGCACACAAGTATTTATTGTCAATTTTTTTTTACTCATTTTTCTGTCGTACTGGCAACAAGATAATTCTTTCAATGAGTTGTGTTATTGTCTGACAATGCTAGGGGAGTTTCTTTTCTCCTGATGAGGTACTTTACCTGCCAGTCAAATAGAAAGATTGCTATAGGCAATAACATATGGTCTAGATATTTGCAACCAGTTGATTTATGAAATAGTTGGGGTGGATCTATAGTAATCTGCCATAATTTGTAATGATCTTTACCATGTTCTATTTATAGCATTGATTTTTAACAGAATTGAGAGTAAGGCAAACATAGTTTTCATTACAAATGCAATTAGACTTTCATAATTTATATTTTTTTAAAGCACTATCTCAGCTCCACCTGGTTTTGATTCATTGCACCTTTCCCACTGATTTCTCTTGTAGCTTGAGGACTTAAGTTGGCTACTAGTGCAGTTTATTTAGAATTTTTTTTTGTGTTGCAACAGAGAGGCAGCTATGTACATGCCAGGGGTTTTTCAACTCATCATTTGTTTAGACACGTGACGGTATCTATCACTTTGTTCATTATGTTTACTTTCGTTCAGGAATCTAGAGCTTGCTCTCTCTCAAAGATGATGATTTATTTTCATCTTAATGCAGGAGCATGCTGCCACTGTGCTGCAGTATTTTCTTGGAATTCAAGCAAAAGCAGCTCTATATCCTTTATTGGTAACATCATCCATTGATTTTGTGGACATGCCTTATTCTTTCTTCATTCCATTTAAAATTCTCGTCAATTAATATGTGGTACATTTTTGTAGCTTTGGATCTGCAGCTACCAAAATTTATTTTCAAAAGCTTGCAGGTGTGTGTAGGGATTTTCTGTGCTTTCATATTTGGTTCTGTGTGGATTTGACATTTTAATGGTACGTTAATCTGGTCTTGCAGTAAATGCGGACGGCTACTATCAGTAAACAAACAGTCAGATTTACTGTTACCTCCTGCAATTCGCCCTTACAAGCAGTTTTGTACATCAAAAATCTCATCGGCTGTGCCCATATCCTCATTGAAAAACCAGCGGTTGGATCCTGTTCAAGCTTTCCACATCGGTTGCTTCTCAGAACTATAG

mRNA sequence

ATGCGCCAGACGCAGCAGCCAGCGCAGGGCCCGACTGCGACGGTGAGCCCTAATACTGCTGCGCCTCATTCCGGCGGCTCTGCAGACGAGGCGCCGCCGAAGCAGGTAGCGCTGGCCATGGATAGACTCGGCCACGCCGCACGCCTCATCGCCGATATTAGGCTCGGCGCCGATCGTCTTCTCGAAGCCTTATGCGTCACTGCACAGCCTCATCAAAGCACCAAGCCTCTGCACTTGTTTCAGAAAGAGGATGCTTCGATGCGGCAACACCTCCAAGACCTTCGTGCAGTTGGAAGGCAGCTTGAAGAGTCTGGCGTTCTCAGTGAATCTCTTCTTTCACGAAGTAATTCTTGGGGTCTACATATGCCTTTAGTGTGCCCTGACGGAGCAGTGGTTGCTTATGCTTGGAAACGTCAACTAGCTGGCCAGGCCGGTGCATCCGCAGTTGACCGGACCAGGTTAGCTCTAAAGGCCTTCACTGACCAGAAAAGACGGTTTTTCCCTCATCTTGACGATGAAAATGGGTCGAATATTGAGCCTGTTCTGAAGAAGCCTTGTTTTTCTCATGGAGTGACAGAAATTAAACAAGAGGAGCTTTATGAATCTAGAACACTGCCAGATATTCTAACATGTATGGAAAAGGAAGTGCCAGATCTTAAGATTTTCACTTACGAGCGATTAGACTGGTTGAAACAAGCTTCAGCACTTCCCTCACAAGCAAATGAGAATTCCATGGGAGCATTGAAAGAACATAACTATCATAGTCCTAGGATGAGCCCTGGATCGCTTGGTGCTACTGCCAAAGATAAAGTTGCTGTAATTGAGTTGATCATCCCTTCTGTCTTTAGAGCTGTAGTATCACTGCATCCAGTTGGTTCTGTTGAACCAGATGCTGTAGCTTTCTTTTCTCCTGATGAGAGAGGCAGCTATGTACATGCCAGGGGTTTTTCAACTCATCATTTGTTTAGACACGTGACGGAGCATGCTGCCACTGTGCTGCAGTATTTTCTTGGAATTCAAGCAAAAGCAGCTCTATATCCTTTATTGCTTTGGATCTGCAGCTACCAAAATTTATTTTCAAAAGCTTGCAGTAAATGCGGACGGCTACTATCAGTAAACAAACAGTCAGATTTACTGTTACCTCCTGCAATTCGCCCTTACAAGCAGTTTTGTACATCAAAAATCTCATCGGCTGTGCCCATATCCTCATTGAAAAACCAGCGGTTGGATCCTGTTCAAGCTTTCCACATCGGTTGCTTCTCAGAACTATAG

Coding sequence (CDS)

ATGCGCCAGACGCAGCAGCCAGCGCAGGGCCCGACTGCGACGGTGAGCCCTAATACTGCTGCGCCTCATTCCGGCGGCTCTGCAGACGAGGCGCCGCCGAAGCAGGTAGCGCTGGCCATGGATAGACTCGGCCACGCCGCACGCCTCATCGCCGATATTAGGCTCGGCGCCGATCGTCTTCTCGAAGCCTTATGCGTCACTGCACAGCCTCATCAAAGCACCAAGCCTCTGCACTTGTTTCAGAAAGAGGATGCTTCGATGCGGCAACACCTCCAAGACCTTCGTGCAGTTGGAAGGCAGCTTGAAGAGTCTGGCGTTCTCAGTGAATCTCTTCTTTCACGAAGTAATTCTTGGGGTCTACATATGCCTTTAGTGTGCCCTGACGGAGCAGTGGTTGCTTATGCTTGGAAACGTCAACTAGCTGGCCAGGCCGGTGCATCCGCAGTTGACCGGACCAGGTTAGCTCTAAAGGCCTTCACTGACCAGAAAAGACGGTTTTTCCCTCATCTTGACGATGAAAATGGGTCGAATATTGAGCCTGTTCTGAAGAAGCCTTGTTTTTCTCATGGAGTGACAGAAATTAAACAAGAGGAGCTTTATGAATCTAGAACACTGCCAGATATTCTAACATGTATGGAAAAGGAAGTGCCAGATCTTAAGATTTTCACTTACGAGCGATTAGACTGGTTGAAACAAGCTTCAGCACTTCCCTCACAAGCAAATGAGAATTCCATGGGAGCATTGAAAGAACATAACTATCATAGTCCTAGGATGAGCCCTGGATCGCTTGGTGCTACTGCCAAAGATAAAGTTGCTGTAATTGAGTTGATCATCCCTTCTGTCTTTAGAGCTGTAGTATCACTGCATCCAGTTGGTTCTGTTGAACCAGATGCTGTAGCTTTCTTTTCTCCTGATGAGAGAGGCAGCTATGTACATGCCAGGGGTTTTTCAACTCATCATTTGTTTAGACACGTGACGGAGCATGCTGCCACTGTGCTGCAGTATTTTCTTGGAATTCAAGCAAAAGCAGCTCTATATCCTTTATTGCTTTGGATCTGCAGCTACCAAAATTTATTTTCAAAAGCTTGCAGTAAATGCGGACGGCTACTATCAGTAAACAAACAGTCAGATTTACTGTTACCTCCTGCAATTCGCCCTTACAAGCAGTTTTGTACATCAAAAATCTCATCGGCTGTGCCCATATCCTCATTGAAAAACCAGCGGTTGGATCCTGTTCAAGCTTTCCACATCGGTTGCTTCTCAGAACTATAG

Protein sequence

MRQTQQPAQGPTATVSPNTAAPHSGGSADEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLQDLRAVGRQLEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEPVLKKPCFSHGVTEIKQEELYESRTLPDILTCMEKEVPDLKIFTYERLDWLKQASALPSQANENSMGALKEHNYHSPRMSPGSLGATAKDKVAVIELIIPSVFRAVVSLHPVGSVEPDAVAFFSPDERGSYVHARGFSTHHLFRHVTEHAATVLQYFLGIQAKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPISSLKNQRLDPVQAFHIGCFSEL
Homology
BLAST of Sgr018216.1 vs. NCBI nr
Match: XP_022151438.1 (mediator of RNA polymerase II transcription subunit 27 [Momordica charantia])

HSP 1 Score: 780.0 bits (2013), Expect = 1.0e-221
Identity = 390/422 (92.42%), Postives = 405/422 (95.97%), Query Frame = 0

Query: 1   MRQTQQPAQGPTATVSPNTAAPHSGGSADEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQQPAQG TATVSPNTAAPHSGGSA+EAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLQDLRAVGRQLEESGVLSESLLSRSNSWGL 120
           LEALCVTA PHQSTKPLHLFQKED SMRQHL+DLRAVG+QLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDE+  N+EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEP 180

Query: 181 VLKKPCFSHGVTEIKQEELYESRTLPDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
            LKKPCFSHGVTEIKQ+EL E RTL DILTCMEKEVPDLKIFTYERLDWLKQA AL SQA
Sbjct: 181 ALKKPCFSHGVTEIKQDELNEPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQA 240

Query: 241 NENSMGALKEHNYHSPRMSPGSLGATAKDKVAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           N+NS+GALKEHNYHSPRM PGSL A++KDKV+VIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSYVHARGFSTHHLFRHVTEHAATVLQYFLGIQAKAALYPLLLWICSYQNLFS 360
           FF+PDERGSYVHARGFS HHLFRHVTEHAATVLQYFLGIQ KAALYPLLLWICSYQNLFS
Sbjct: 301 FFAPDERGSYVHARGFSVHHLFRHVTEHAATVLQYFLGIQEKAALYPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPISSLKNQRLDPVQAFHIGCF 420
           KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTS++ SAVPISSLK+QR DPVQAFHIGCF
Sbjct: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVPISSLKDQRSDPVQAFHIGCF 420

Query: 421 SE 423
           SE
Sbjct: 421 SE 422

BLAST of Sgr018216.1 vs. NCBI nr
Match: XP_022940393.1 (mediator of RNA polymerase II transcription subunit 27 [Cucurbita moschata])

HSP 1 Score: 768.5 bits (1983), Expect = 3.1e-218
Identity = 385/423 (91.02%), Postives = 403/423 (95.27%), Query Frame = 0

Query: 1   MRQTQQPAQGPTATVSPNTAAPHSGGSADEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQ PAQG TATVSP T   HS GSA+EAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLQDLRAVGRQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQSTKPLHLFQKEDASMRQHL DLRAVG+QLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLD+ENGSN+EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 VLKKPCFSHGVTEIKQEELYESRTLPDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
             KKPCFSHGVT++KQEEL +S +L DILTCMEKEVPDLKIFTYERLDWLKQASALPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSMGALKEHNYHSPRMSPGSLGATAKDKVAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENS+GALKEHNYHSPRMSP SL AT+ DK AVIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSYVHARGFSTHHLFRHVTEHAATVLQYFLGIQAKAALYPLLLWICSYQNLFS 360
           FFSPDERGS+VHARGFS HHLFRHVTEHAATVLQYF+GIQAKAAL+PLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPISSLKNQRLDPVQAFHIGCF 420
           KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISS VP++SLK+Q+LD VQAFHIGCF
Sbjct: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSVVPVTSLKDQKLDSVQAFHIGCF 420

Query: 421 SEL 424
           +EL
Sbjct: 421 TEL 423

BLAST of Sgr018216.1 vs. NCBI nr
Match: XP_023539656.1 (mediator of RNA polymerase II transcription subunit 27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 768.5 bits (1983), Expect = 3.1e-218
Identity = 386/423 (91.25%), Postives = 403/423 (95.27%), Query Frame = 0

Query: 1   MRQTQQPAQGPTATVSPNTAAPHSGGSADEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQ PAQG TATVSP T   HS GSA+EAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLQDLRAVGRQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQSTKPLHLFQKEDASMRQHL DLRAVG+QLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEP 180

Query: 181 VLKKPCFSHGVTEIKQEELYESRTLPDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
             KKPCFSHGVT++KQEEL +S +L DILTC+EKEVPDLKIFTYERLDWLKQASAL SQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCLEKEVPDLKIFTYERLDWLKQASALSSQA 240

Query: 241 NENSMGALKEHNYHSPRMSPGSLGATAKDKVAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENS+GALKEHNYHSPRMSP SL AT+ DK AVIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSYVHARGFSTHHLFRHVTEHAATVLQYFLGIQAKAALYPLLLWICSYQNLFS 360
           FFSPDERGS+VHARGFS HHLFRHVTEHAATVLQYF+GIQAKAAL+PLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPISSLKNQRLDPVQAFHIGCF 420
           KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVP++SLK+Q+LD VQAFHIGCF
Sbjct: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCF 420

Query: 421 SEL 424
           +EL
Sbjct: 421 TEL 423

BLAST of Sgr018216.1 vs. NCBI nr
Match: XP_023005694.1 (mediator of RNA polymerase II transcription subunit 27 [Cucurbita maxima])

HSP 1 Score: 766.9 bits (1979), Expect = 9.0e-218
Identity = 384/423 (90.78%), Postives = 402/423 (95.04%), Query Frame = 0

Query: 1   MRQTQQPAQGPTATVSPNTAAPHSGGSADEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQ PAQG TATVSP T   HS GSA+EAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLQDLRAVGRQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQSTKPLHLFQKEDASMRQHL DLRAVG+QLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLD+ENGSN+EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 VLKKPCFSHGVTEIKQEELYESRTLPDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
             KKPCFSHGVT++KQEEL +S +L DILTCMEKEVPDLKIFTYERLDWLKQAS LPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASVLPSQA 240

Query: 241 NENSMGALKEHNYHSPRMSPGSLGATAKDKVAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENS+G LKEHNYHSPRMSP SL AT+ DK AVIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NENSIGTLKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSYVHARGFSTHHLFRHVTEHAATVLQYFLGIQAKAALYPLLLWICSYQNLFS 360
           FFSPDERGS+VHARGFS HHLFRHVTEHAATVLQYF+GIQAKAAL+PLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPISSLKNQRLDPVQAFHIGCF 420
           KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVP++SLK+Q+LD VQAFHIGCF
Sbjct: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCF 420

Query: 421 SEL 424
           +EL
Sbjct: 421 TEL 423

BLAST of Sgr018216.1 vs. NCBI nr
Match: XP_038904949.1 (mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida] >XP_038904950.1 mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida] >XP_038904951.1 mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida] >XP_038904952.1 mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida])

HSP 1 Score: 763.1 bits (1969), Expect = 1.3e-216
Identity = 381/421 (90.50%), Postives = 400/421 (95.01%), Query Frame = 0

Query: 1   MRQTQQPAQGPTATVSPNTAAPHSGGSADEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQQPAQG TATVSPNTA PHS  SAD+APPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQQPAQGQTATVSPNTAPPHSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLQDLRAVGRQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQSTKPLHLFQKEDASMRQHL DLRAVG+QLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL+DENGSN EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEP 180

Query: 181 VLKKPCFSHGVTEIKQEELYESRTLPDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
            LKKPCFSHGVT++KQ EL +SR+L +ILTCMEKEVP+LKIFTYERLDWLKQASALPSQA
Sbjct: 181 ALKKPCFSHGVTDVKQNELNDSRSLSEILTCMEKEVPELKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSMGALKEHNYHSPRMSPGSLGATAKDKVAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENS+G LKEH+YHSPRMSPGSLGAT  DK  VIEL+IPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NENSIGVLKEHSYHSPRMSPGSLGATPVDKAGVIELVIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSYVHARGFSTHHLFRHVTEHAATVLQYFLGIQAKAALYPLLLWICSYQNLFS 360
           FFSPDERGS+VHARGFS HHLFRHVTEHAATVLQYFLG QAKAALYPLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFLGNQAKAALYPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPISSLKNQRLDPVQAFHIGCF 420
           KACSKCG+LLSVNKQSDLLLPPA+RPYKQFC SK SS+VPI+SLK+Q+LD VQAFHI CF
Sbjct: 361 KACSKCGQLLSVNKQSDLLLPPAVRPYKQFCASKNSSSVPITSLKDQKLDSVQAFHIDCF 420

Query: 421 S 422
           S
Sbjct: 421 S 421

BLAST of Sgr018216.1 vs. ExPASy Swiss-Prot
Match: Q8RWM3 (Mediator of RNA polymerase II transcription subunit 27 OS=Arabidopsis thaliana OX=3702 GN=MED27 PE=1 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 3.1e-128
Identity = 242/402 (60.20%), Postives = 301/402 (74.88%), Query Frame = 0

Query: 18  NTAAPHSGGSADEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPL 77
           N A   +  S  +APPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V +QP  +  PL
Sbjct: 12  NPAEAANNQSESDAPPKQVAQAMERLNQAARVIADIRLGADRILEAMFVASQPRHTDMPL 71

Query: 78  HLFQKEDASMRQHLQDLRAVGRQLEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWK 137
            LF +EDASMRQHLQDLR +G++LEESGVL+ESL SRSNSWGLHMPLVCPDGAVVAYAWK
Sbjct: 72  QLFLREDASMRQHLQDLRLIGKKLEESGVLTESLRSRSNSWGLHMPLVCPDGAVVAYAWK 131

Query: 138 RQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEPVLKKPCFSHGVTEIKQE 197
           RQLAGQAGASAVDRTRLALKAFTDQKRRFFPH+DD  G  +EP  KK   SH + E  +E
Sbjct: 132 RQLAGQAGASAVDRTRLALKAFTDQKRRFFPHIDD--GLKMEPSSKKHRASHLLLENGRE 191

Query: 198 ELYESRTLPDILTCMEKEVPDLKIFTYERLDWLKQASALPSQANENSMGALKEHNYHSPR 257
           E  + +TLPDI + +EK VP +K+ TY RL+WLK+A++LP   +++   A K     S +
Sbjct: 192 EPVDYKTLPDIQSRLEKLVPSVKVSTYGRLNWLKRANSLPGSGSDDPTEASKPIFQSSSK 251

Query: 258 MSPGSLGATAKDKVAVIELIIPSVFRAVVSLHPVGSVEPDAVAFFSPDERGSYVHARGFS 317
           +  G L     DK+AVIEL  PS+FRA+VSL P GSV+PDAVAFFSPDE GSY+HARGFS
Sbjct: 252 LRSG-LQTEVVDKIAVIELSFPSLFRAIVSLSPAGSVDPDAVAFFSPDEGGSYLHARGFS 311

Query: 318 THHLFRHVTEHAATVLQYFLGIQAKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSD 377
            +H+++H+TEHAAT LQYFLG     ALY LLLWICS++++FSK C+KCGRLL+++K+S 
Sbjct: 312 VYHVYKHITEHAATALQYFLGFGTGTALYSLLLWICSFESVFSKPCTKCGRLLAMDKKSA 371

Query: 378 LLLPPAIRPYKQFCTSKISSAVPISSLKNQRLDPVQAFHIGC 420
           L+LPP  R Y++         +P++      LD  +A+H  C
Sbjct: 372 LILPPLHRAYQE---------LPLA----LNLDVCEAYHSSC 397

BLAST of Sgr018216.1 vs. ExPASy TrEMBL
Match: A0A6J1DC62 (mediator of RNA polymerase II transcription subunit 27 OS=Momordica charantia OX=3673 GN=LOC111019372 PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 5.0e-222
Identity = 390/422 (92.42%), Postives = 405/422 (95.97%), Query Frame = 0

Query: 1   MRQTQQPAQGPTATVSPNTAAPHSGGSADEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQQPAQG TATVSPNTAAPHSGGSA+EAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLQDLRAVGRQLEESGVLSESLLSRSNSWGL 120
           LEALCVTA PHQSTKPLHLFQKED SMRQHL+DLRAVG+QLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDE+  N+EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEP 180

Query: 181 VLKKPCFSHGVTEIKQEELYESRTLPDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
            LKKPCFSHGVTEIKQ+EL E RTL DILTCMEKEVPDLKIFTYERLDWLKQA AL SQA
Sbjct: 181 ALKKPCFSHGVTEIKQDELNEPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQA 240

Query: 241 NENSMGALKEHNYHSPRMSPGSLGATAKDKVAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           N+NS+GALKEHNYHSPRM PGSL A++KDKV+VIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSYVHARGFSTHHLFRHVTEHAATVLQYFLGIQAKAALYPLLLWICSYQNLFS 360
           FF+PDERGSYVHARGFS HHLFRHVTEHAATVLQYFLGIQ KAALYPLLLWICSYQNLFS
Sbjct: 301 FFAPDERGSYVHARGFSVHHLFRHVTEHAATVLQYFLGIQEKAALYPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPISSLKNQRLDPVQAFHIGCF 420
           KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTS++ SAVPISSLK+QR DPVQAFHIGCF
Sbjct: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVPISSLKDQRSDPVQAFHIGCF 420

Query: 421 SE 423
           SE
Sbjct: 421 SE 422

BLAST of Sgr018216.1 vs. ExPASy TrEMBL
Match: A0A6J1FP59 (mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita moschata OX=3662 GN=LOC111446014 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.5e-218
Identity = 385/423 (91.02%), Postives = 403/423 (95.27%), Query Frame = 0

Query: 1   MRQTQQPAQGPTATVSPNTAAPHSGGSADEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQ PAQG TATVSP T   HS GSA+EAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLQDLRAVGRQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQSTKPLHLFQKEDASMRQHL DLRAVG+QLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLD+ENGSN+EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 VLKKPCFSHGVTEIKQEELYESRTLPDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
             KKPCFSHGVT++KQEEL +S +L DILTCMEKEVPDLKIFTYERLDWLKQASALPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSMGALKEHNYHSPRMSPGSLGATAKDKVAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENS+GALKEHNYHSPRMSP SL AT+ DK AVIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSYVHARGFSTHHLFRHVTEHAATVLQYFLGIQAKAALYPLLLWICSYQNLFS 360
           FFSPDERGS+VHARGFS HHLFRHVTEHAATVLQYF+GIQAKAAL+PLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPISSLKNQRLDPVQAFHIGCF 420
           KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISS VP++SLK+Q+LD VQAFHIGCF
Sbjct: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSVVPVTSLKDQKLDSVQAFHIGCF 420

Query: 421 SEL 424
           +EL
Sbjct: 421 TEL 423

BLAST of Sgr018216.1 vs. ExPASy TrEMBL
Match: A0A6J1KZZ9 (mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita maxima OX=3661 GN=LOC111498620 PE=3 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 4.3e-218
Identity = 384/423 (90.78%), Postives = 402/423 (95.04%), Query Frame = 0

Query: 1   MRQTQQPAQGPTATVSPNTAAPHSGGSADEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQ PAQG TATVSP T   HS GSA+EAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLQDLRAVGRQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQSTKPLHLFQKEDASMRQHL DLRAVG+QLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLD+ENGSN+EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 VLKKPCFSHGVTEIKQEELYESRTLPDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
             KKPCFSHGVT++KQEEL +S +L DILTCMEKEVPDLKIFTYERLDWLKQAS LPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASVLPSQA 240

Query: 241 NENSMGALKEHNYHSPRMSPGSLGATAKDKVAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENS+G LKEHNYHSPRMSP SL AT+ DK AVIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NENSIGTLKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSYVHARGFSTHHLFRHVTEHAATVLQYFLGIQAKAALYPLLLWICSYQNLFS 360
           FFSPDERGS+VHARGFS HHLFRHVTEHAATVLQYF+GIQAKAAL+PLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPISSLKNQRLDPVQAFHIGCF 420
           KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVP++SLK+Q+LD VQAFHIGCF
Sbjct: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCF 420

Query: 421 SEL 424
           +EL
Sbjct: 421 TEL 423

BLAST of Sgr018216.1 vs. ExPASy TrEMBL
Match: A0A1S3B7L6 (mediator of RNA polymerase II transcription subunit 27 OS=Cucumis melo OX=3656 GN=LOC103486676 PE=3 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 9.7e-210
Identity = 370/422 (87.68%), Postives = 394/422 (93.36%), Query Frame = 0

Query: 1   MRQTQQPAQGPTATVSPNTAAPHSGGSADEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  QQP+QG TATVSPNT   HS  SAD+APPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRHIQQPSQGQTATVSPNT---HSPSSADDAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLQDLRAVGRQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQS+KPLHLFQKEDASMRQHL DLRAVG+QLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL+DEN SN EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEP 180

Query: 181 VLKKPCFSHGVTEIKQEELYESRTLPDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
            LKKPCFSHGVT++KQ EL +SR+L DIL+C+EKEVP+LKIFTYERLDWLKQASALPSQA
Sbjct: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCVEKEVPELKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSMGALKEHNYHSPRMSPGSLGATAKDKVAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENS+GALKEH+YHSPRMSPG+LGAT  DK  VIELIIPSVFRAVVSLHP+GSVEPDA+A
Sbjct: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPIGSVEPDALA 300

Query: 301 FFSPDERGSYVHARGFSTHHLFRHVTEHAATVLQYFLGIQAKAALYPLLLWICSYQNLFS 360
           FFSPDERGS+VHARGFS +H+FRHVTEHAATVLQYFLG Q KAALYPLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNYHVFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPISSLKNQRLDPVQAFHIGCF 420
           KACSKCGR LSVNKQSDLLLPP +RPYKQFC SK SSAVPI+SLK+Q+LD VQAFHI CF
Sbjct: 361 KACSKCGRRLSVNKQSDLLLPPVVRPYKQFCASKNSSAVPITSLKDQKLDSVQAFHIDCF 419

Query: 421 SE 423
           SE
Sbjct: 421 SE 419

BLAST of Sgr018216.1 vs. ExPASy TrEMBL
Match: A0A0A0LGT1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G781560 PE=3 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 2.8e-209
Identity = 371/422 (87.91%), Postives = 393/422 (93.13%), Query Frame = 0

Query: 1   MRQTQQPAQGPTATVSPNTAAPHSGGSADEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  QQP+QG TATVSP+T   HS  S D+APPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLQDLRAVGRQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQS+KPLHLFQKEDASMRQHL DLRAVG+QLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL+DEN SN EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEP 180

Query: 181 VLKKPCFSHGVTEIKQEELYESRTLPDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
            LKKPCFSHGVT++KQ EL +SR+L DIL+CMEKEVP+LKIFTYERLDWLKQASALPSQA
Sbjct: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSMGALKEHNYHSPRMSPGSLGATAKDKVAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENS+GALKEH+YHSPRMSPG+LGAT  DK  VIELIIPSVFRAVVSLHPVGSVEPDA+A
Sbjct: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA 300

Query: 301 FFSPDERGSYVHARGFSTHHLFRHVTEHAATVLQYFLGIQAKAALYPLLLWICSYQNLFS 360
           FFSPDERGS+VHARGFS +HLFRHVTEHAATVLQYFLG Q KAALYPLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPISSLKNQRLDPVQAFHIGCF 420
           KACSKCGR LSVNKQSDLLLPPA+RPYKQFC SK SS VPI+SLK+Q+LD VQAFHI CF
Sbjct: 361 KACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITSLKDQKLDSVQAFHIDCF 419

Query: 421 SE 423
           SE
Sbjct: 421 SE 419

BLAST of Sgr018216.1 vs. TAIR 10
Match: AT3G09180.1 (CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med27 (InterPro:IPR021627); Has 112 Blast hits to 112 proteins in 38 species: Archae - 0; Bacteria - 0; Metazoa - 79; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink). )

HSP 1 Score: 459.9 bits (1182), Expect = 2.2e-129
Identity = 242/402 (60.20%), Postives = 301/402 (74.88%), Query Frame = 0

Query: 18  NTAAPHSGGSADEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPL 77
           N A   +  S  +APPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V +QP  +  PL
Sbjct: 12  NPAEAANNQSESDAPPKQVAQAMERLNQAARVIADIRLGADRILEAMFVASQPRHTDMPL 71

Query: 78  HLFQKEDASMRQHLQDLRAVGRQLEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWK 137
            LF +EDASMRQHLQDLR +G++LEESGVL+ESL SRSNSWGLHMPLVCPDGAVVAYAWK
Sbjct: 72  QLFLREDASMRQHLQDLRLIGKKLEESGVLTESLRSRSNSWGLHMPLVCPDGAVVAYAWK 131

Query: 138 RQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEPVLKKPCFSHGVTEIKQE 197
           RQLAGQAGASAVDRTRLALKAFTDQKRRFFPH+DD  G  +EP  KK   SH + E  +E
Sbjct: 132 RQLAGQAGASAVDRTRLALKAFTDQKRRFFPHIDD--GLKMEPSSKKHRASHLLLENGRE 191

Query: 198 ELYESRTLPDILTCMEKEVPDLKIFTYERLDWLKQASALPSQANENSMGALKEHNYHSPR 257
           E  + +TLPDI + +EK VP +K+ TY RL+WLK+A++LP   +++   A K     S +
Sbjct: 192 EPVDYKTLPDIQSRLEKLVPSVKVSTYGRLNWLKRANSLPGSGSDDPTEASKPIFQSSSK 251

Query: 258 MSPGSLGATAKDKVAVIELIIPSVFRAVVSLHPVGSVEPDAVAFFSPDERGSYVHARGFS 317
           +  G L     DK+AVIEL  PS+FRA+VSL P GSV+PDAVAFFSPDE GSY+HARGFS
Sbjct: 252 LRSG-LQTEVVDKIAVIELSFPSLFRAIVSLSPAGSVDPDAVAFFSPDEGGSYLHARGFS 311

Query: 318 THHLFRHVTEHAATVLQYFLGIQAKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSD 377
            +H+++H+TEHAAT LQYFLG     ALY LLLWICS++++FSK C+KCGRLL+++K+S 
Sbjct: 312 VYHVYKHITEHAATALQYFLGFGTGTALYSLLLWICSFESVFSKPCTKCGRLLAMDKKSA 371

Query: 378 LLLPPAIRPYKQFCTSKISSAVPISSLKNQRLDPVQAFHIGC 420
           L+LPP  R Y++         +P++      LD  +A+H  C
Sbjct: 372 LILPPLHRAYQE---------LPLA----LNLDVCEAYHSSC 397

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151438.11.0e-22192.42mediator of RNA polymerase II transcription subunit 27 [Momordica charantia][more]
XP_022940393.13.1e-21891.02mediator of RNA polymerase II transcription subunit 27 [Cucurbita moschata][more]
XP_023539656.13.1e-21891.25mediator of RNA polymerase II transcription subunit 27 [Cucurbita pepo subsp. pe... [more]
XP_023005694.19.0e-21890.78mediator of RNA polymerase II transcription subunit 27 [Cucurbita maxima][more]
XP_038904949.11.3e-21690.50mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida] >XP_0... [more]
Match NameE-valueIdentityDescription
Q8RWM33.1e-12860.20Mediator of RNA polymerase II transcription subunit 27 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1DC625.0e-22292.42mediator of RNA polymerase II transcription subunit 27 OS=Momordica charantia OX... [more]
A0A6J1FP591.5e-21891.02mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita moschata OX=... [more]
A0A6J1KZZ94.3e-21890.78mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita maxima OX=36... [more]
A0A1S3B7L69.7e-21087.68mediator of RNA polymerase II transcription subunit 27 OS=Cucumis melo OX=3656 G... [more]
A0A0A0LGT12.8e-20987.91Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G781560 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G09180.12.2e-12960.20CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med27 (InterPro:IPR021627);... [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021627Mediator complex, subunit Med27PFAMPF11571Med27coord: 302..419
e-value: 1.3E-22
score: 80.0
IPR021627Mediator complex, subunit Med27PANTHERPTHR1313034 KDA TRANSCRIPTIONAL CO-ACTIVATOR-RELATEDcoord: 21..391
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availablePANTHERPTHR13130:SF5BNAC03G36240D PROTEINcoord: 21..391

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Sgr018216Sgr018216gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sgr018216.1.exon1Sgr018216.1.exon1exon
Sgr018216.1.exon2Sgr018216.1.exon2exon
Sgr018216.1.exon3Sgr018216.1.exon3exon
Sgr018216.1.exon4Sgr018216.1.exon4exon
Sgr018216.1.exon5Sgr018216.1.exon5exon
Sgr018216.1.exon6Sgr018216.1.exon6exon
Sgr018216.1.exon7Sgr018216.1.exon7exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Sgr018216.1cds.Sgr018216.1CDS
cds.Sgr018216.1cds.Sgr018216.1_2CDS
cds.Sgr018216.1cds.Sgr018216.1_3CDS
cds.Sgr018216.1cds.Sgr018216.1_4CDS
cds.Sgr018216.1cds.Sgr018216.1_5CDS
cds.Sgr018216.1cds.Sgr018216.1_6CDS
cds.Sgr018216.1cds.Sgr018216.1_7CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Sgr018216.1Sgr018216.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0045893 positive regulation of transcription, DNA-templated
cellular_component GO:0016592 mediator complex
molecular_function GO:0003713 transcription coactivator activity