Sgr017047.1 (mRNA) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr017047.1
Type	mRNA
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Polcalcin Jun o
Location	tig00153017: 1068850 .. 1069353 (+)
Sequence length	504
RNA-Seq Expression	Sgr017047.1
Synteny	Sgr017047.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGTTCAAATCACTCTTTTCCCGGAAGAAGAAATCGGATACCTCGAGCGACTCATCGCTTGGATCTACGGCGGTTCCGATGCTTGGATCTCGAACGCCGTCTCTCAATTCTCGAGCGCAAATAGCGGAGCTCGAGCAAGTGTTCAAGAAATTCGATGTCAATGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATTATGGGGAGCCTAGGGCAGCCAGCGACGGAAGAGGAGATTCAGAACATGATCAAGGAGGTCGATGCGGACGGCGACGGCTTCATTGATCTCAACGAGTTCATCGAGCTGAACACCAAGGGCGTCGATTCCGACGAGGTGCTGGAGAATCTGAAGGACGCATTCTCCGTCTACGACATCGACGGGAACGGATCGATAACGGCGGAGGAATTGCACGAGGTGTTGAAGAGTTTGGGCGAGGAGTGTTCGCTGGCGGATTGCCGGAAGATGATCACCGGCGTCGACAGAAACGGGACGGTATGA

mRNA sequence

ATGGGGTTCAAATCACTCTTTTCCCGGAAGAAGAAATCGGATACCTCGAGCGACTCATCGCTTGGATCTACGGCGGTTCCGATGCTTGGATCTCGAACGCCGTCTCTCAATTCTCGAGCGCAAATAGCGGAGCTCGAGCAAGTGTTCAAGAAATTCGATGTCAATGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATTATGGGGAGCCTAGGGCAGCCAGCGACGGAAGAGGAGATTCAGAACATGATCAAGGAGGTCGATGCGGACGGCGACGGCTTCATTGATCTCAACGAGTTCATCGAGCTGAACACCAAGGGCGTCGATTCCGACGAGGTGCTGGAGAATCTGAAGGACGCATTCTCCGTCTACGACATCGACGGGAACGGATCGATAACGGCGGAGGAATTGCACGAGGTGTTGAAGAGTTTGGGCGAGGAGTGTTCGCTGGCGGATTGCCGGAAGATGATCACCGGCGTCGACAGAAACGGGACGGTATGA

Coding sequence (CDS)

ATGGGGTTCAAATCACTCTTTTCCCGGAAGAAGAAATCGGATACCTCGAGCGACTCATCGCTTGGATCTACGGCGGTTCCGATGCTTGGATCTCGAACGCCGTCTCTCAATTCTCGAGCGCAAATAGCGGAGCTCGAGCAAGTGTTCAAGAAATTCGATGTCAATGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATTATGGGGAGCCTAGGGCAGCCAGCGACGGAAGAGGAGATTCAGAACATGATCAAGGAGGTCGATGCGGACGGCGACGGCTTCATTGATCTCAACGAGTTCATCGAGCTGAACACCAAGGGCGTCGATTCCGACGAGGTGCTGGAGAATCTGAAGGACGCATTCTCCGTCTACGACATCGACGGGAACGGATCGATAACGGCGGAGGAATTGCACGAGGTGTTGAAGAGTTTGGGCGAGGAGTGTTCGCTGGCGGATTGCCGGAAGATGATCACCGGCGTCGACAGAAACGGGACGGTATGA

Protein sequence

MGFKSLFSRKKKSDTSSDSSLGSTAVPMLGSRTPSLNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVDRNGTV

Homology

[Close]

BLAST of Sgr017047.1 vs. NCBI nr
Match: XP_023513887.1 (probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 300.4 bits (768), Expect = 9.4e-78
Identity = 151/165 (91.52%), Postives = 161/165 (97.58%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSSLGSTAVPMLGSRTPSLNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDS LGST VP+LGSRTPS NSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKD 120
           CSSELGSIMGSLGQPATEEE+QNMIKEVDADGDG+IDL+EFIELNTKGVDSDEVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVDRNG 166
           AFSVYD+DGNGSITAEELHEVLKSLG++CSLADCR MI+GVDRNG
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNG 165

[Close]

BLAST of Sgr017047.1 vs. NCBI nr
Match: XP_022963580.1 (probable calcium-binding protein CML25 [Cucurbita moschata])

HSP 1 Score: 300.4 bits (768), Expect = 9.4e-78
Identity = 151/165 (91.52%), Postives = 161/165 (97.58%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSSLGSTAVPMLGSRTPSLNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDS LGST VP+LGSRTPS NSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKD 120
           CSSELGSIMGSLGQPATEEE+QNMIKEVDADGDG+IDL+EFIELNTKGVDSDEVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVDRNG 166
           AFSVYD+DGNGSITAEELHEVLKSLG++CSLADCR MI+GVDRNG
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNG 165

[Close]

BLAST of Sgr017047.1 vs. NCBI nr
Match: XP_022990613.1 (probable calcium-binding protein CML25 [Cucurbita maxima])

HSP 1 Score: 300.4 bits (768), Expect = 9.4e-78
Identity = 151/165 (91.52%), Postives = 161/165 (97.58%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSSLGSTAVPMLGSRTPSLNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDS LGST VP+LGSRTPS NSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKD 120
           CSSELGSIMGSLGQPATEEE+QNMIKEVDADGDG+IDL+EFIELNTKGVDSDEVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVDRNG 166
           AFSVYD+DGNGSITAEELHEVLKSLG++CSLADCR MI+GVDRNG
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNG 165

[Close]

BLAST of Sgr017047.1 vs. NCBI nr
Match: XP_022146331.1 (probable calcium-binding protein CML25 [Momordica charantia])

HSP 1 Score: 298.9 bits (764), Expect = 2.7e-77
Identity = 154/165 (93.33%), Postives = 158/165 (95.76%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSSLGSTAVPMLGSRTPSLNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD SSDS  GSTAVP+LGSRTPS N R QIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKD 120
            SSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDL+EFIELNTKGVDS+EVLENLKD
Sbjct: 61  SSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVDRNG 166
           AFSVYDIDGNGSITAEELHEVLKSLG+ECSLADCRKMITGVDRNG
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNG 165

[Close]

BLAST of Sgr017047.1 vs. NCBI nr
Match: KAG6601861.1 (putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032560.1 putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 298.5 bits (763), Expect = 3.6e-77
Identity = 150/165 (90.91%), Postives = 160/165 (96.97%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSSLGSTAVPMLGSRTPSLNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDS LGST VP+LGSRTPS NSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKD 120
           CSSELGSIMG LGQPATEEE+QNMIKEVDADGDG+IDL+EFIELNTKGVDSDEVL+NLKD
Sbjct: 61  CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVDRNG 166
           AFSVYD+DGNGSITAEELHEVLKSLG++CSLADCR MI+GVDRNG
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNG 165

[Close]

BLAST of Sgr017047.1 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.4e-47
Identity = 97/164 (59.15%), Postives = 127/164 (77.44%), Query Frame = 0


Query: 6   LFSRKKKSDTSSDSSLGSTAVPMLGSRTPSL----NSRAQIAELEQVFKKFDVNGDGKIC 65
           +F++ + S+  S S++G      +G+ +P L    + + +I ELE VFKKFDVNGDGKI 
Sbjct: 1   MFNKNQGSNGGSSSNVG------IGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKIS 60

Query: 66  SSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKDA 125
           S ELG+IM SLG    EEE++  I E+D  GDG+I+  EF+ELNTKG+D ++VLENLKDA
Sbjct: 61  SKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDA 120

Query: 126 FSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVDRNG 166
           FSVYDIDGNGSI+AEELHEVL+SLG+ECS+A+CRKMI GVD++G
Sbjct: 121 FSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDG 158

[Close]

BLAST of Sgr017047.1 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 4.0e-31
Identity = 68/123 (55.28%), Postives = 90/123 (73.17%), Query Frame = 0


Query: 44  ELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIE 103
           E+E+VF+KFD NGDG+I  SELG++  SLG  AT++E+  M+ E DADGDGFI L+EF  
Sbjct: 40  EMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFAA 99

Query: 104 LN-TKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVD 163
           LN T   D+  V E+L+ AF V+D DGNG+I+A EL  VL  LGE+ ++  CR+MI GVD
Sbjct: 100 LNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVD 159

Query: 164 RNG 166
           +NG
Sbjct: 160 QNG 162

[Close]

BLAST of Sgr017047.1 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 129.4 bits (324), Expect = 3.8e-29
Identity = 63/124 (50.81%), Postives = 88/124 (70.97%), Query Frame = 0


Query: 42  IAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEF 101
           + ELE+VFKKFD NGDGKI  SEL  I+ SLG    E E++ M++E DADGDG++ L EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEF 83

Query: 102 IELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGV 161
           ++LN KG      +++LK+AF V+D D NGSI+A EL   L+S+GE C++ + + +I  V
Sbjct: 84  VDLNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNV 143

Query: 162 DRNG 166
           D+NG
Sbjct: 144 DKNG 143

[Close]

BLAST of Sgr017047.1 vs. ExPASy Swiss-Prot
Match: A0T2M3 (Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.1e-28
Identity = 61/124 (49.19%), Postives = 87/124 (70.16%), Query Frame = 0


Query: 42  IAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEF 101
           + ELE+VFKKFD NGDGKI  SEL  I+ S+G    E E++ M++E D DGDG++ L EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSMGSEVDEAEVKAMMEEADTDGDGYVSLQEF 83

Query: 102 IELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGV 161
           ++LN KG      +++LK+AF V+D D NG+I+  EL E LKS+GE C++ + + +I  V
Sbjct: 84  VDLNIKGA----TVKDLKNAFKVFDRDCNGTISPAELCETLKSVGEPCTIEESKNIIHNV 143

Query: 162 DRNG 166
           D+NG
Sbjct: 144 DKNG 143

[Close]

BLAST of Sgr017047.1 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.2e-28
Identity = 61/137 (44.53%), Postives = 95/137 (69.34%), Query Frame = 0


Query: 31  SRTPSLNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEEIQNMIKEVDA 90
           S+  S N    + ++++VF++FD N DGKI   EL  ++G+L   A++EE + M+KE D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 91  DGDGFIDLNEFIEL--NTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGEE 150
           DG+GFIDL+EF+ L   +    ++  + +LK+AF +YD+D NG I+A ELH V+K+LGE+
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 151 CSLADCRKMITGVDRNG 166
           CS+ DC++MI  VD +G
Sbjct: 122 CSIQDCQRMINKVDSDG 138

[Close]

BLAST of Sgr017047.1 vs. ExPASy TrEMBL
Match: A0A6J1JTS9 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111487441 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 4.5e-78
Identity = 151/165 (91.52%), Postives = 161/165 (97.58%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSSLGSTAVPMLGSRTPSLNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDS LGST VP+LGSRTPS NSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKD 120
           CSSELGSIMGSLGQPATEEE+QNMIKEVDADGDG+IDL+EFIELNTKGVDSDEVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVDRNG 166
           AFSVYD+DGNGSITAEELHEVLKSLG++CSLADCR MI+GVDRNG
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNG 165

[Close]

BLAST of Sgr017047.1 vs. ExPASy TrEMBL
Match: A0A6J1HID9 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111463873 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 4.5e-78
Identity = 151/165 (91.52%), Postives = 161/165 (97.58%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSSLGSTAVPMLGSRTPSLNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDS LGST VP+LGSRTPS NSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKD 120
           CSSELGSIMGSLGQPATEEE+QNMIKEVDADGDG+IDL+EFIELNTKGVDSDEVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVDRNG 166
           AFSVYD+DGNGSITAEELHEVLKSLG++CSLADCR MI+GVDRNG
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNG 165

[Close]

BLAST of Sgr017047.1 vs. ExPASy TrEMBL
Match: A0A6J1CZ20 (probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC111015568 PE=4 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 1.3e-77
Identity = 154/165 (93.33%), Postives = 158/165 (95.76%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSSLGSTAVPMLGSRTPSLNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD SSDS  GSTAVP+LGSRTPS N R QIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKD 120
            SSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDL+EFIELNTKGVDS+EVLENLKD
Sbjct: 61  SSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVDRNG 166
           AFSVYDIDGNGSITAEELHEVLKSLG+ECSLADCRKMITGVDRNG
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNG 165

[Close]

BLAST of Sgr017047.1 vs. ExPASy TrEMBL
Match: A0A0A0KST2 (Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 6.4e-72
Identity = 144/165 (87.27%), Postives = 154/165 (93.33%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSSLGSTAVPMLGSRTPSLNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKS +S+DS LGST +P+LGSRT       QIAELEQVF KFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRT-------QIAELEQVFNKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKD 120
           CSSELGSIMGSLGQPATEEE+QNMIKEVDADGDG+IDL+EFIELNTKGVDSDEVLENLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVDRNG 166
           AFSVYDIDGNGSITAEELHEVLKSLG++CSLADCRKMITGVD+NG
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNG 158

[Close]

BLAST of Sgr017047.1 vs. ExPASy TrEMBL
Match: A0A6J1FFM7 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111445291 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 5.4e-71
Identity = 144/166 (86.75%), Postives = 155/166 (93.37%), Query Frame = 0


Query: 1   MGFKSLFSRK-KKSDTSSDSSLGSTAVPMLGSRTPSLNSRAQIAELEQVFKKFDVNGDGK 60
           MGFKSLFS K KKSD S+DS L ST VP+LGSRTPS NSRAQ+A+L+QVFKKFDVNGDGK
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK 60

Query: 61  ICSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLK 120
           ICSSELGSIM SLGQPATEEEIQ+MI+EVDADGDG+IDL+EFIELNTK VDSDEVL NLK
Sbjct: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120

Query: 121 DAFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVDRNG 166
           DAFSVYDIDGNGSITAEEL EVLKSLG+ECSLADCRKMITGVD+NG
Sbjct: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNG 166

[Close]

BLAST of Sgr017047.1 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 190.7 bits (483), Expect = 9.8e-49
Identity = 97/164 (59.15%), Postives = 127/164 (77.44%), Query Frame = 0


Query: 6   LFSRKKKSDTSSDSSLGSTAVPMLGSRTPSL----NSRAQIAELEQVFKKFDVNGDGKIC 65
           +F++ + S+  S S++G      +G+ +P L    + + +I ELE VFKKFDVNGDGKI 
Sbjct: 1   MFNKNQGSNGGSSSNVG------IGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKIS 60

Query: 66  SSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKDA 125
           S ELG+IM SLG    EEE++  I E+D  GDG+I+  EF+ELNTKG+D ++VLENLKDA
Sbjct: 61  SKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDA 120

Query: 126 FSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMITGVDRNG 166
           FSVYDIDGNGSI+AEELHEVL+SLG+ECS+A+CRKMI GVD++G
Sbjct: 121 FSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDG 158

[Close]

BLAST of Sgr017047.1 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 126.3 bits (316), Expect = 2.3e-29
Identity = 61/137 (44.53%), Postives = 95/137 (69.34%), Query Frame = 0


Query: 31  SRTPSLNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEEIQNMIKEVDA 90
           S+  S N    + ++++VF++FD N DGKI   EL  ++G+L   A++EE + M+KE D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 91  DGDGFIDLNEFIEL--NTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGEE 150
           DG+GFIDL+EF+ L   +    ++  + +LK+AF +YD+D NG I+A ELH V+K+LGE+
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 151 CSLADCRKMITGVDRNG 166
           CS+ DC++MI  VD +G
Sbjct: 122 CSIQDCQRMINKVDSDG 138

[Close]

BLAST of Sgr017047.1 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 124.8 bits (312), Expect = 6.6e-29
Identity = 60/126 (47.62%), Postives = 92/126 (73.02%), Query Frame = 0


Query: 44  ELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLNEFIE 103
           ++++VF++FD NGDGKI   EL  ++ +L   A+ EE   M+K+ D DG+GFIDL+EF+ 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 104 LNTKGV----DSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGEECSLADCRKMIT 163
           L   G+    ++   + +LK+AF +YD+DGNG I+A+ELH V+K+LGE+CS+ DC+KMI+
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 164 GVDRNG 166
            VD +G
Sbjct: 137 KVDIDG 142

[Close]

BLAST of Sgr017047.1 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 115.2 bits (287), Expect = 5.2e-26
Identity = 62/143 (43.36%), Postives = 87/143 (60.84%), Query Frame = 0


Query: 23  STAVPMLGSRTPSLNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEEIQ 82
           ++A P     TP+    A   EL++VF +FD NGDGKI   ELG +  ++G   TE E+ 
Sbjct: 2   ASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELN 61

Query: 83  NMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVL 142
            +++EVD D DG+I+L+EF  L      + E+    +DAF +YD D NG I+A ELH+VL
Sbjct: 62  RVLEEVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVL 121

Query: 143 KSLGEECSLADCRKMITGVDRNG 166
             LG  CS+ DC +MI  VD +G
Sbjct: 122 NRLGMSCSVEDCTRMIGPVDADG 140

[Close]

BLAST of Sgr017047.1 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 115.2 bits (287), Expect = 5.2e-26
Identity = 62/143 (43.36%), Postives = 87/143 (60.84%), Query Frame = 0


Query: 23  STAVPMLGSRTPSLNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEEIQ 82
           ++A P     TP+    A   EL++VF +FD NGDGKI   ELG +  ++G   TE E+ 
Sbjct: 2   ASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELN 61

Query: 83  NMIKEVDADGDGFIDLNEFIELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVL 142
            +++EVD D DG+I+L+EF  L      + E+    +DAF +YD D NG I+A ELH+VL
Sbjct: 62  RVLEEVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVL 121

Query: 143 KSLGEECSLADCRKMITGVDRNG 166
             LG  CS+ DC +MI  VD +G
Sbjct: 122 NRLGMSCSVEDCTRMIGPVDADG 140

The following BLAST results are available for this feature:

BLAST of Sgr017047.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023513887.1	9.4e-78	91.52	probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo]	[more]
XP_022963580.1	9.4e-78	91.52	probable calcium-binding protein CML25 [Cucurbita moschata]	[more]
XP_022990613.1	9.4e-78	91.52	probable calcium-binding protein CML25 [Cucurbita maxima]	[more]
XP_022146331.1	2.7e-77	93.33	probable calcium-binding protein CML25 [Momordica charantia]	[more]
KAG6601861.1	3.6e-77	90.91	putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of Sgr017047.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYK2	1.4e-47	59.15	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q8RZB5	4.0e-31	55.28	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
O64943	3.8e-29	50.81	Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2	[more]
A0T2M3	1.1e-28	49.19	Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1	[more]
Q9C8Y1	3.2e-28	44.53	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]

BLAST of Sgr017047.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JTS9	4.5e-78	91.52	probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114874...	[more]
A0A6J1HID9	4.5e-78	91.52	probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1CZ20	1.3e-77	93.33	probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A0A0KST2	6.4e-72	87.27	Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1	[more]
A0A6J1FFM7	5.4e-71	86.75	probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

BLAST of Sgr017047.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24620.1	9.8e-49	59.15	EF hand calcium-binding protein family	[more]
AT1G66400.1	2.3e-29	44.53	calmodulin like 23	[more]
AT5G37770.1	6.6e-29	47.62	EF hand calcium-binding protein family	[more]
AT1G18210.1	5.2e-26	43.36	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	5.2e-26	43.36	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 44..72 e-value: 4.4E-6 score: 36.2 coord: 117..145 e-value: 1.3E-5 score: 34.7 coord: 80..108 e-value: 1.4E-4 score: 31.3
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 44..103 e-value: 1.7E-13 score: 50.8
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 118..145 e-value: 3.7E-7 score: 29.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 40..75 score: 15.071314
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 113..148 score: 15.461849
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 76..111 score: 13.704439
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 44..104 e-value: 4.58956E-21 score: 79.5141
None	No IPR available	GENE3D	1.10.238.10		coord: 108..166 e-value: 2.1E-15 score: 58.5 coord: 30..107 e-value: 7.3E-25 score: 89.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..33
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..33
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 20..165
None	No IPR available	PANTHER	PTHR23050:SF382	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 20..165
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 126..138
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 53..65
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 89..101
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 35..165

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sgr017047	Sgr017047	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sgr017047.1.exon1	Sgr017047.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Sgr017047.1	cds.Sgr017047.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sgr017047.1	Sgr017047.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity