Sgr016974.1 (mRNA) Monk fruit (Qingpiguo) v1

BLAST of Sgr016974.1 vs. NCBI nr
Match: XP_022146442.1 (uncharacterized protein LOC111015657 isoform X1 [Momordica charantia])

HSP 1 Score: 735.7 bits (1898), Expect = 2.2e-208
Identity = 370/417 (88.73%), Postives = 383/417 (91.85%), Query Frame = 0

Query: 1   MSSFTAASASLWTPRLTTSSKFSKFNSSPPCFRVVCSGGFQQQYAAKDLRFLLHDAMDSF 60
           M+S T ASASLW P LT SSKFSKFNSSPPCFRVVCSG FQ  YAAKDL F LHDAMDS 
Sbjct: 1   MNSITVASASLWNPILTNSSKFSKFNSSPPCFRVVCSGEFQHHYAAKDLHFHLHDAMDSS 60

Query: 61  GIDSTHAKEARKGFLTQIQYLSNIEREISISINRRVDLAKAALYIATEDDSLVSHSSVPL 120
           GIDST+AKEARKGFLTQI+Y SNIE+E SISINRRVDLAKAALYIA EDDSLVSHSSVPL
Sbjct: 61  GIDSTYAKEARKGFLTQIRYFSNIEKETSISINRRVDLAKAALYIAAEDDSLVSHSSVPL 120

Query: 121 PIDAYVRRVNDLSMGYCTHYKSSFNLSPENFLESIERYMYVMKGFRRTSSKTRSEPRALY 180
           PIDA++ R++DLSMGYCTHYKSSFNLSPE+FLESIERYMYV KGFRRTSS T+SE RALY
Sbjct: 121 PIDAFINRLSDLSMGYCTHYKSSFNLSPESFLESIERYMYVTKGFRRTSSTTQSESRALY 180

Query: 181 LHTVLTHRTGSAALLSLIYSEILKMLRFWSLLDFDVEIYHPHDDYSLPTGYHKQKSKESD 240
           LHTVLTHRTGSAALLSL+YSEILKMLR WSLLDFDVEIYHPHD YSLPTGYHKQKSKESD
Sbjct: 181 LHTVLTHRTGSAALLSLVYSEILKMLRLWSLLDFDVEIYHPHDGYSLPTGYHKQKSKESD 240

Query: 241 QPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADAANCSDRSNVIEESGFQLASA 300
           QPHIITTQSLLVEILSNLKESFWPFQQN SRSLFLRAAD ANCSDRSN IEESGFQLASA
Sbjct: 241 QPHIITTQSLLVEILSNLKESFWPFQQNNSRSLFLRAADVANCSDRSNAIEESGFQLASA 300

Query: 301 RAAQHRLERGVWTSVRFGDMRRALSACERLILLDVDSKELRDYSILLYHCGFYEQSLEYL 360
           +AAQHRLERGVWTSVRFGDMRRALSACERLILLDVD KELRDYSILLYHCGFYEQSLEYL
Sbjct: 301 KAAQHRLERGVWTSVRFGDMRRALSACERLILLDVDPKELRDYSILLYHCGFYEQSLEYL 360

Query: 361 KLYQEIKSSSSSPDALSCQEEEAVVNLMKRLTLIMMEDGWSRPSYARNFIGKNSEPW 418
           KLYQE KS  S  D +SCQEEEAV NLMKRL LIMMEDGWS PSY RNFIGKNSEPW
Sbjct: 361 KLYQETKSCLSPSDTISCQEEEAVNNLMKRLALIMMEDGWSSPSYTRNFIGKNSEPW 417

BLAST of Sgr016974.1 vs. NCBI nr
Match: XP_038875536.1 (uncharacterized protein LOC120067957 [Benincasa hispida])

HSP 1 Score: 697.2 bits (1798), Expect = 8.6e-197
Identity = 362/426 (84.98%), Postives = 379/426 (88.97%), Query Frame = 0

Query: 1   MSSFTAASASLWTPRLTTSS--KFSKFNSS-----PPCFRVVCSGGF--QQQYAAKDLRF 60
           MSSFT ASASL  PRL +SS  KFSKFNSS     PPCFRVVCS GF  QQ  + KD +F
Sbjct: 1   MSSFT-ASASLCIPRLISSSSFKFSKFNSSSPHSTPPCFRVVCSAGFLPQQPNSLKDFQF 60

Query: 61  LLHDAMDSFGIDSTHAKEARKGFLTQIQYLSNIEREISISINRRVDLAKAALYIATEDDS 120
           LLHDAMDS GIDSTHAKEARKGFL+QI YLS +ER+ SISINRRVDLAKAALYIA EDDS
Sbjct: 61  LLHDAMDSSGIDSTHAKEARKGFLSQIHYLSKMERDTSISINRRVDLAKAALYIAAEDDS 120

Query: 121 LVSHSSVPLPIDAYVRRVNDLSMGYCTHYKSSFNLSPENFLESIERYMYVMKGFRRTSSK 180
           LVSHSSVPLP+DA++ R++DLSMGYCTHYKSSFN SPE FLESIE YMYVMKGFRR SSK
Sbjct: 121 LVSHSSVPLPVDAFINRISDLSMGYCTHYKSSFNSSPEIFLESIEWYMYVMKGFRRASSK 180

Query: 181 TRSEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRFWSLLDFDVEIYHPHDDYSLPTGY 240
            +SEPRALYLHTVLTHRTGSAALLSLIYSEILKMLR WSLLDFDVE+YHPHDDYSLPTGY
Sbjct: 181 AQSEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRLWSLLDFDVEVYHPHDDYSLPTGY 240

Query: 241 HKQKSKESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADAANCSDRSNVIE 300
           HK KSKESDQPHI+TTQ+LLVEILSNLKESFWPFQQNQSRSLFLRAAD ANCSD  N  E
Sbjct: 241 HKLKSKESDQPHIMTTQTLLVEILSNLKESFWPFQQNQSRSLFLRAADVANCSDSLNAFE 300

Query: 301 ESGFQLASARAAQHRLERGVWTSVRFGDMRRALSACERLILLDVDSKELRDYSILLYHCG 360
           ESGFQLASA+AAQHRLERGVWTSVR+GDMRRALSACERLILLDVDSKELRDYSILLYHCG
Sbjct: 301 ESGFQLASAKAAQHRLERGVWTSVRYGDMRRALSACERLILLDVDSKELRDYSILLYHCG 360

Query: 361 FYEQSLEYLKLYQEIKSSSSSPDALSCQEEEAVVNLMKRLTLIMMEDGWSRPSYARNFIG 418
           FYEQSLEYLKLYQE KSSSS    LSCQEEEAV NLM RL LIMMEDGWSRPS  R FIG
Sbjct: 361 FYEQSLEYLKLYQETKSSSSPTSKLSCQEEEAVDNLMIRLALIMMEDGWSRPSLPRKFIG 420

BLAST of Sgr016974.1 vs. NCBI nr
Match: KAG6579123.1 (hypothetical protein SDJN03_23571, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 694.1 bits (1790), Expect = 7.3e-196
Identity = 358/428 (83.64%), Postives = 380/428 (88.79%), Query Frame = 0

Query: 1   MSSFTAASASLWTPRLTTSSKFSKFN--------SSPP---CFRVVCSGGFQQQYAAKDL 60
           M+SFT  SA L  PRL +SSK SKFN        SSP     FRVVCSGGF+Q  A KD 
Sbjct: 1   MASFT--SAFLCIPRLISSSKLSKFNFSSSSSSSSSPSTSLSFRVVCSGGFRQPDAPKDF 60

Query: 61  RFLLHDAMDSFGIDSTHAKEARKGFLTQIQYLSNIEREISISINRRVDLAKAALYIATED 120
           RFLLHDA+DS GIDST+AKEARKGFLTQI YLSNIERE SISINR VDLAKAALYIA ED
Sbjct: 61  RFLLHDALDSSGIDSTYAKEARKGFLTQIHYLSNIERETSISINRCVDLAKAALYIAAED 120

Query: 121 DSLVSHSSVPLPIDAYVRRVNDLSMGYCTHYKSSFNLSPENFLESIERYMYVMKGFRRTS 180
           DSLVSHSSVPLP+DA+V R+NDLSMGYCTHYKSSFNLSPE+ LESIERY+YVMKGFRRTS
Sbjct: 121 DSLVSHSSVPLPVDAFVHRINDLSMGYCTHYKSSFNLSPESLLESIERYLYVMKGFRRTS 180

Query: 181 SKTRSEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRFWSLLDFDVEIYHPHDDYSLPT 240
            K ++EPRALYLHTVLTHRTGSAALLSLIYSEILKMLR WSLLDFDVEIYHPHDD+SLPT
Sbjct: 181 CKAQTEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRLWSLLDFDVEIYHPHDDFSLPT 240

Query: 241 GYHKQKSKESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADAANCSDRSNV 300
            YHK K +ESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAAD ANCSDRSN 
Sbjct: 241 AYHKLKGRESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADVANCSDRSNA 300

Query: 301 IEESGFQLASARAAQHRLERGVWTSVRFGDMRRALSACERLILLDVDSKELRDYSILLYH 360
            EESGFQLASA+AAQHRLERGVWTSVR+GDMRRALSACERLILLDVD KELRDYSILLYH
Sbjct: 301 TEESGFQLASAKAAQHRLERGVWTSVRYGDMRRALSACERLILLDVDPKELRDYSILLYH 360

Query: 361 CGFYEQSLEYLKLYQEIKSSSSSPDALSCQEEEAVVNLMKRLTLIMMEDGWSRPSYARNF 418
           CGFYEQSLEYLKLY+E K+SSS  D LSCQEEEAV +L+KRL LIMMEDGWSRP++AR F
Sbjct: 361 CGFYEQSLEYLKLYRETKNSSSPTDTLSCQEEEAVDHLIKRLALIMMEDGWSRPTFARKF 420

BLAST of Sgr016974.1 vs. NCBI nr
Match: XP_023530796.1 (uncharacterized protein LOC111793214 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 688.3 bits (1775), Expect = 4.0e-194
Identity = 353/423 (83.45%), Postives = 375/423 (88.65%), Query Frame = 0

Query: 1   MSSFTAAS--ASLWTPRLTTSSKFSKFNSS-----PPCFRVVCSGGFQQQYAAKDLRFLL 60
           M SFT AS  ASLW PRL+ SSKFSKFNSS      P FRVVCSGG +   A +D  F+L
Sbjct: 1   MDSFTVASSFASLWIPRLSASSKFSKFNSSSSHSIQPSFRVVCSGGSRHNIAPQDFHFIL 60

Query: 61  HDAMDSFGIDSTHAKEARKGFLTQIQYLSNIEREISISINRRVDLAKAALYIATEDDSLV 120
           HDAMDS GID++++KEARKGFLTQIQYLSNIERE SISINRRVDLAKAALYIA EDDSLV
Sbjct: 61  HDAMDSSGIDASYSKEARKGFLTQIQYLSNIERETSISINRRVDLAKAALYIAAEDDSLV 120

Query: 121 SHSSVPLPIDAYVRRVNDLSMGYCTHYKSSFNLSPENFLESIERYMYVMKGFRRTSSKTR 180
           SHSSVPLPIDA++  + DLSMGYCTHYKSSFNLSPE+FLESIERYMYV KGFRRTSSK +
Sbjct: 121 SHSSVPLPIDAFIHSLADLSMGYCTHYKSSFNLSPESFLESIERYMYVTKGFRRTSSKAQ 180

Query: 181 SEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRFWSLLDFDVEIYHPHDDYSLPTGYHK 240
            EP+ALYLHTVLTH TGS+ LLSLIYSEILKMLR WSLLDFDVEIYHPHD+YSLPTGYHK
Sbjct: 181 LEPQALYLHTVLTHGTGSSTLLSLIYSEILKMLRLWSLLDFDVEIYHPHDNYSLPTGYHK 240

Query: 241 QKSKESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADAANCSDRSNVIEES 300
            KSKESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRA D ANCSDRSN IEES
Sbjct: 241 LKSKESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAVDVANCSDRSNAIEES 300

Query: 301 GFQLASARAAQHRLERGVWTSVRFGDMRRALSACERLILLDVDSKELRDYSILLYHCGFY 360
           GFQLASA+AAQHRLERGVWTS R+GDMRRAL+ACERLILLDVD KELRDYSILLYHCGFY
Sbjct: 301 GFQLASAKAAQHRLERGVWTSRRYGDMRRALAACERLILLDVDMKELRDYSILLYHCGFY 360

Query: 361 EQSLEYLKLYQEIKSSSSSPDALSCQEEEAVVNLMKRLTLIMMEDGWSRPSYARNFIGKN 417
           EQSLEYLKLYQE KSSSS  D  S +EEEAV NLMKRL LIM+EDGWS PSYAR FIGKN
Sbjct: 361 EQSLEYLKLYQETKSSSSPTDTSSSEEEEAVENLMKRLALIMIEDGWSTPSYARKFIGKN 420

BLAST of Sgr016974.1 vs. NCBI nr
Match: XP_022938946.1 (uncharacterized protein LOC111445003 isoform X2 [Cucurbita moschata])

HSP 1 Score: 685.3 bits (1767), Expect = 3.4e-193
Identity = 355/420 (84.52%), Postives = 375/420 (89.29%), Query Frame = 0

Query: 1   MSSFTAASASLWTPRLTTSSKFSKFN------SSPP---CFRVVCSGGFQQQYAAKDLRF 60
           M+SFT  SASL  PRL +SSK SKFN      SSP     FRVVCSGGF+Q  A KD RF
Sbjct: 1   MASFT--SASLCIPRLISSSKLSKFNFSSSSSSSPSTSLSFRVVCSGGFRQPDAPKDFRF 60

Query: 61  LLHDAMDSFGIDSTHAKEARKGFLTQIQYLSNIEREISISINRRVDLAKAALYIATEDDS 120
           LLHDA+DS GIDST+AKEARKGFLTQI YLSNIERE SISINR VDLAKAALYIA EDDS
Sbjct: 61  LLHDALDSSGIDSTYAKEARKGFLTQIHYLSNIERETSISINRCVDLAKAALYIAAEDDS 120

Query: 121 LVSHSSVPLPIDAYVRRVNDLSMGYCTHYKSSFNLSPENFLESIERYMYVMKGFRRTSSK 180
           LVSHSSVPLP+DA+V R+NDLSMGYCTHYKSSFNLSPE+ LESIERY+YVMKGFRRTS K
Sbjct: 121 LVSHSSVPLPVDAFVHRINDLSMGYCTHYKSSFNLSPESLLESIERYLYVMKGFRRTSCK 180

Query: 181 TRSEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRFWSLLDFDVEIYHPHDDYSLPTGY 240
            ++EPRALYLHTVLTHRTGSAALLSLIYSEILKMLR WSLLDFDVEIYHPHDD+SLPT Y
Sbjct: 181 AQTEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRLWSLLDFDVEIYHPHDDFSLPTAY 240

Query: 241 HKQKSKESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADAANCSDRSNVIE 300
           HK K +ESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAAD ANCSDRSN  E
Sbjct: 241 HKLKGRESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADVANCSDRSNAAE 300

Query: 301 ESGFQLASARAAQHRLERGVWTSVRFGDMRRALSACERLILLDVDSKELRDYSILLYHCG 360
           ESGFQLASA+AAQHRLERGVWTSVR+GDMRRALSACERLILLDVD KELRDYSILLYHCG
Sbjct: 301 ESGFQLASAKAAQHRLERGVWTSVRYGDMRRALSACERLILLDVDPKELRDYSILLYHCG 360

Query: 361 FYEQSLEYLKLYQEIKSSSSSPDALSCQEEEAVVNLMKRLTLIMMEDGWSRPSYARNFIG 412
           FYEQSLEYLKLYQE K+SSS  D LSCQEEEAV +LMKRL LIMMEDGWSRP++AR FIG
Sbjct: 361 FYEQSLEYLKLYQETKNSSSPTDTLSCQEEEAVDHLMKRLALIMMEDGWSRPTFARKFIG 418

BLAST of Sgr016974.1 vs. ExPASy TrEMBL
Match: A0A6J1CZL0 (uncharacterized protein LOC111015657 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015657 PE=4 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 1.1e-208
Identity = 370/417 (88.73%), Postives = 383/417 (91.85%), Query Frame = 0

Query: 1   MSSFTAASASLWTPRLTTSSKFSKFNSSPPCFRVVCSGGFQQQYAAKDLRFLLHDAMDSF 60
           M+S T ASASLW P LT SSKFSKFNSSPPCFRVVCSG FQ  YAAKDL F LHDAMDS 
Sbjct: 1   MNSITVASASLWNPILTNSSKFSKFNSSPPCFRVVCSGEFQHHYAAKDLHFHLHDAMDSS 60

Query: 61  GIDSTHAKEARKGFLTQIQYLSNIEREISISINRRVDLAKAALYIATEDDSLVSHSSVPL 120
           GIDST+AKEARKGFLTQI+Y SNIE+E SISINRRVDLAKAALYIA EDDSLVSHSSVPL
Sbjct: 61  GIDSTYAKEARKGFLTQIRYFSNIEKETSISINRRVDLAKAALYIAAEDDSLVSHSSVPL 120

Query: 121 PIDAYVRRVNDLSMGYCTHYKSSFNLSPENFLESIERYMYVMKGFRRTSSKTRSEPRALY 180
           PIDA++ R++DLSMGYCTHYKSSFNLSPE+FLESIERYMYV KGFRRTSS T+SE RALY
Sbjct: 121 PIDAFINRLSDLSMGYCTHYKSSFNLSPESFLESIERYMYVTKGFRRTSSTTQSESRALY 180

Query: 181 LHTVLTHRTGSAALLSLIYSEILKMLRFWSLLDFDVEIYHPHDDYSLPTGYHKQKSKESD 240
           LHTVLTHRTGSAALLSL+YSEILKMLR WSLLDFDVEIYHPHD YSLPTGYHKQKSKESD
Sbjct: 181 LHTVLTHRTGSAALLSLVYSEILKMLRLWSLLDFDVEIYHPHDGYSLPTGYHKQKSKESD 240

Query: 241 QPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADAANCSDRSNVIEESGFQLASA 300
           QPHIITTQSLLVEILSNLKESFWPFQQN SRSLFLRAAD ANCSDRSN IEESGFQLASA
Sbjct: 241 QPHIITTQSLLVEILSNLKESFWPFQQNNSRSLFLRAADVANCSDRSNAIEESGFQLASA 300

Query: 301 RAAQHRLERGVWTSVRFGDMRRALSACERLILLDVDSKELRDYSILLYHCGFYEQSLEYL 360
           +AAQHRLERGVWTSVRFGDMRRALSACERLILLDVD KELRDYSILLYHCGFYEQSLEYL
Sbjct: 301 KAAQHRLERGVWTSVRFGDMRRALSACERLILLDVDPKELRDYSILLYHCGFYEQSLEYL 360

Query: 361 KLYQEIKSSSSSPDALSCQEEEAVVNLMKRLTLIMMEDGWSRPSYARNFIGKNSEPW 418
           KLYQE KS  S  D +SCQEEEAV NLMKRL LIMMEDGWS PSY RNFIGKNSEPW
Sbjct: 361 KLYQETKSCLSPSDTISCQEEEAVNNLMKRLALIMMEDGWSSPSYTRNFIGKNSEPW 417

BLAST of Sgr016974.1 vs. ExPASy TrEMBL
Match: A0A6J1FEJ1 (uncharacterized protein LOC111445003 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445003 PE=4 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.6e-193
Identity = 355/420 (84.52%), Postives = 375/420 (89.29%), Query Frame = 0

Query: 1   MSSFTAASASLWTPRLTTSSKFSKFN------SSPP---CFRVVCSGGFQQQYAAKDLRF 60
           M+SFT  SASL  PRL +SSK SKFN      SSP     FRVVCSGGF+Q  A KD RF
Sbjct: 1   MASFT--SASLCIPRLISSSKLSKFNFSSSSSSSPSTSLSFRVVCSGGFRQPDAPKDFRF 60

Query: 61  LLHDAMDSFGIDSTHAKEARKGFLTQIQYLSNIEREISISINRRVDLAKAALYIATEDDS 120
           LLHDA+DS GIDST+AKEARKGFLTQI YLSNIERE SISINR VDLAKAALYIA EDDS
Sbjct: 61  LLHDALDSSGIDSTYAKEARKGFLTQIHYLSNIERETSISINRCVDLAKAALYIAAEDDS 120

Query: 121 LVSHSSVPLPIDAYVRRVNDLSMGYCTHYKSSFNLSPENFLESIERYMYVMKGFRRTSSK 180
           LVSHSSVPLP+DA+V R+NDLSMGYCTHYKSSFNLSPE+ LESIERY+YVMKGFRRTS K
Sbjct: 121 LVSHSSVPLPVDAFVHRINDLSMGYCTHYKSSFNLSPESLLESIERYLYVMKGFRRTSCK 180

Query: 181 TRSEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRFWSLLDFDVEIYHPHDDYSLPTGY 240
            ++EPRALYLHTVLTHRTGSAALLSLIYSEILKMLR WSLLDFDVEIYHPHDD+SLPT Y
Sbjct: 181 AQTEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRLWSLLDFDVEIYHPHDDFSLPTAY 240

Query: 241 HKQKSKESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADAANCSDRSNVIE 300
           HK K +ESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAAD ANCSDRSN  E
Sbjct: 241 HKLKGRESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADVANCSDRSNAAE 300

Query: 301 ESGFQLASARAAQHRLERGVWTSVRFGDMRRALSACERLILLDVDSKELRDYSILLYHCG 360
           ESGFQLASA+AAQHRLERGVWTSVR+GDMRRALSACERLILLDVD KELRDYSILLYHCG
Sbjct: 301 ESGFQLASAKAAQHRLERGVWTSVRYGDMRRALSACERLILLDVDPKELRDYSILLYHCG 360

Query: 361 FYEQSLEYLKLYQEIKSSSSSPDALSCQEEEAVVNLMKRLTLIMMEDGWSRPSYARNFIG 412
           FYEQSLEYLKLYQE K+SSS  D LSCQEEEAV +LMKRL LIMMEDGWSRP++AR FIG
Sbjct: 361 FYEQSLEYLKLYQETKNSSSPTDTLSCQEEEAVDHLMKRLALIMMEDGWSRPTFARKFIG 418

BLAST of Sgr016974.1 vs. ExPASy TrEMBL
Match: A0A6J1JZH6 (uncharacterized protein LOC111489694 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489694 PE=4 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 2.8e-193
Identity = 353/422 (83.65%), Postives = 373/422 (88.39%), Query Frame = 0

Query: 1   MSSFTAASASLWTPRLTTSSKFSKFNSSPP-----------CFRVVCSGGFQQQYAAKDL 60
           M+SFT  SASL  PRL +SSK SKFNSS              FRVVCSGGF+Q    KD 
Sbjct: 1   MASFT--SASLCIPRLISSSKLSKFNSSSSSSSSSSPSTSLSFRVVCSGGFRQPDGPKDF 60

Query: 61  RFLLHDAMDSFGIDSTHAKEARKGFLTQIQYLSNIEREISISINRRVDLAKAALYIATED 120
           RFLLHDA+DS GIDST+AKEARKGFLTQI YLSNIERE SISINR VDLAKAALYIA ED
Sbjct: 61  RFLLHDALDSSGIDSTYAKEARKGFLTQIHYLSNIERETSISINRGVDLAKAALYIAAED 120

Query: 121 DSLVSHSSVPLPIDAYVRRVNDLSMGYCTHYKSSFNLSPENFLESIERYMYVMKGFRRTS 180
           DSLVSHSSVPLP+DA+V R+NDLSMGYCTHYKSSFNLSPE+ LESIERY+YVMKGFRRTS
Sbjct: 121 DSLVSHSSVPLPVDAFVHRINDLSMGYCTHYKSSFNLSPESLLESIERYLYVMKGFRRTS 180

Query: 181 SKTRSEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRFWSLLDFDVEIYHPHDDYSLPT 240
            K ++EPRALYLHTVLTHRTGSAALLSLIYSEILKMLR WSLLDFDVEIYHPHDD+SLPT
Sbjct: 181 CKAQTEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRLWSLLDFDVEIYHPHDDFSLPT 240

Query: 241 GYHKQKSKESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADAANCSDRSNV 300
            YHK K KESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAAD ANCSDRSN 
Sbjct: 241 AYHKLKGKESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADVANCSDRSNA 300

Query: 301 IEESGFQLASARAAQHRLERGVWTSVRFGDMRRALSACERLILLDVDSKELRDYSILLYH 360
            EESGFQLASA+AAQHRLERGVWTSVR+GDMRRALSACERLILLDVD KELRDYSILLYH
Sbjct: 301 TEESGFQLASAKAAQHRLERGVWTSVRYGDMRRALSACERLILLDVDPKELRDYSILLYH 360

Query: 361 CGFYEQSLEYLKLYQEIKSSSSSPDALSCQEEEAVVNLMKRLTLIMMEDGWSRPSYARNF 412
           CG+YEQSLEYLKLYQE K+SSS  D LSCQEEEAV +LMKRL LIMMEDGWSRP++AR F
Sbjct: 361 CGYYEQSLEYLKLYQETKNSSSPTDTLSCQEEEAVDHLMKRLALIMMEDGWSRPTFARKF 420

BLAST of Sgr016974.1 vs. ExPASy TrEMBL
Match: A0A6J1GQR6 (uncharacterized protein LOC111456240 OS=Cucurbita moschata OX=3662 GN=LOC111456240 PE=4 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 4.8e-193
Identity = 352/423 (83.22%), Postives = 374/423 (88.42%), Query Frame = 0

Query: 1   MSSFTAAS--ASLWTPRLTTSSKFSKFNSS-----PPCFRVVCSGGFQQQYAAKDLRFLL 60
           M SFT AS  ASLW PRL+ SSKFSKF+SS      P FRVVCSGG +   A +D  F+L
Sbjct: 3   MDSFTVASSFASLWIPRLSASSKFSKFSSSSSHSIQPRFRVVCSGGSRHNIAPQDFHFIL 62

Query: 61  HDAMDSFGIDSTHAKEARKGFLTQIQYLSNIEREISISINRRVDLAKAALYIATEDDSLV 120
           HDAMDS GID+++AKEARKGFLTQIQYLSNIERE SISINRRVDLAKAALYIA EDDSLV
Sbjct: 63  HDAMDSSGIDASYAKEARKGFLTQIQYLSNIERETSISINRRVDLAKAALYIAAEDDSLV 122

Query: 121 SHSSVPLPIDAYVRRVNDLSMGYCTHYKSSFNLSPENFLESIERYMYVMKGFRRTSSKTR 180
           SHSSVPLPIDA++  + DLSMGYCTHYKSSFNLSPE+FLESIERYMYV KGFRRTSSK +
Sbjct: 123 SHSSVPLPIDAFIHSLADLSMGYCTHYKSSFNLSPESFLESIERYMYVTKGFRRTSSKAQ 182

Query: 181 SEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRFWSLLDFDVEIYHPHDDYSLPTGYHK 240
            EP+ALYLHTVLTH TGS+A LSLIYSEILKMLR WSLLDFDVEIYHPHDDYSLPTGYHK
Sbjct: 183 LEPQALYLHTVLTHGTGSSAQLSLIYSEILKMLRLWSLLDFDVEIYHPHDDYSLPTGYHK 242

Query: 241 QKSKESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADAANCSDRSNVIEES 300
            KSKESDQPHIITTQSLLVEILSNLK SFWPFQQNQSRSLFLRA D ANC DRSN IEES
Sbjct: 243 LKSKESDQPHIITTQSLLVEILSNLKGSFWPFQQNQSRSLFLRAVDVANCCDRSNAIEES 302

Query: 301 GFQLASARAAQHRLERGVWTSVRFGDMRRALSACERLILLDVDSKELRDYSILLYHCGFY 360
           GFQLASA+AAQHRLERG+WTS R+GDMRRAL+ACERLILLDVD KELRDYSILLYHCGFY
Sbjct: 303 GFQLASAKAAQHRLERGLWTSRRYGDMRRALAACERLILLDVDMKELRDYSILLYHCGFY 362

Query: 361 EQSLEYLKLYQEIKSSSSSPDALSCQEEEAVVNLMKRLTLIMMEDGWSRPSYARNFIGKN 417
           EQSLEYLKLYQE KSSSS  D LS +EEEAV NLMKRL LIM+EDGWS PSYAR FIGKN
Sbjct: 363 EQSLEYLKLYQETKSSSSPTDMLSSKEEEAVENLMKRLALIMIEDGWSTPSYARKFIGKN 422

BLAST of Sgr016974.1 vs. ExPASy TrEMBL
Match: A0A6J1FL90 (uncharacterized protein LOC111445003 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445003 PE=4 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 8.1e-193
Identity = 354/419 (84.49%), Postives = 374/419 (89.26%), Query Frame = 0

Query: 1   MSSFTAASASLWTPRLTTSSKFSKFN------SSPP---CFRVVCSGGFQQQYAAKDLRF 60
           M+SFT  SASL  PRL +SSK SKFN      SSP     FRVVCSGGF+Q  A KD RF
Sbjct: 1   MASFT--SASLCIPRLISSSKLSKFNFSSSSSSSPSTSLSFRVVCSGGFRQPDAPKDFRF 60

Query: 61  LLHDAMDSFGIDSTHAKEARKGFLTQIQYLSNIEREISISINRRVDLAKAALYIATEDDS 120
           LLHDA+DS GIDST+AKEARKGFLTQI YLSNIERE SISINR VDLAKAALYIA EDDS
Sbjct: 61  LLHDALDSSGIDSTYAKEARKGFLTQIHYLSNIERETSISINRCVDLAKAALYIAAEDDS 120

Query: 121 LVSHSSVPLPIDAYVRRVNDLSMGYCTHYKSSFNLSPENFLESIERYMYVMKGFRRTSSK 180
           LVSHSSVPLP+DA+V R+NDLSMGYCTHYKSSFNLSPE+ LESIERY+YVMKGFRRTS K
Sbjct: 121 LVSHSSVPLPVDAFVHRINDLSMGYCTHYKSSFNLSPESLLESIERYLYVMKGFRRTSCK 180

Query: 181 TRSEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRFWSLLDFDVEIYHPHDDYSLPTGY 240
            ++EPRALYLHTVLTHRTGSAALLSLIYSEILKMLR WSLLDFDVEIYHPHDD+SLPT Y
Sbjct: 181 AQTEPRALYLHTVLTHRTGSAALLSLIYSEILKMLRLWSLLDFDVEIYHPHDDFSLPTAY 240

Query: 241 HKQKSKESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADAANCSDRSNVIE 300
           HK K +ESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAAD ANCSDRSN  E
Sbjct: 241 HKLKGRESDQPHIITTQSLLVEILSNLKESFWPFQQNQSRSLFLRAADVANCSDRSNAAE 300

Query: 301 ESGFQLASARAAQHRLERGVWTSVRFGDMRRALSACERLILLDVDSKELRDYSILLYHCG 360
           ESGFQLASA+AAQHRLERGVWTSVR+GDMRRALSACERLILLDVD KELRDYSILLYHCG
Sbjct: 301 ESGFQLASAKAAQHRLERGVWTSVRYGDMRRALSACERLILLDVDPKELRDYSILLYHCG 360

Query: 361 FYEQSLEYLKLYQEIKSSSSSPDALSCQEEEAVVNLMKRLTLIMMEDGWSRPSYARNFI 411
           FYEQSLEYLKLYQE K+SSS  D LSCQEEEAV +LMKRL LIMMEDGWSRP++AR FI
Sbjct: 361 FYEQSLEYLKLYQETKNSSSPTDTLSCQEEEAVDHLMKRLALIMMEDGWSRPTFARKFI 417

BLAST of Sgr016974.1 vs. TAIR 10
Match: AT4G19160.2 (unknown protein; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 51.6 bits (122), Expect = 1.8e-06
Identity = 95/430 (22.09%), Postives = 169/430 (39.30%), Query Frame = 0

Query: 3   SFTAASASLWTPRLTTSSKFSKFNSSPPCFRVVCSGGFQQQYAAKDLRFLLHDAMDSFGI 62
           S+T     +  PR  T+S      S+ P FR   S   +     K+ + +   A   F  
Sbjct: 51  SWTGIEKKIPFPRKKTASA-----SAYPLFR---SQHTKDSSRPKNYKEVTKSARQMFAR 110

Query: 63  DSTHAKEARKGFLTQIQYLSNIEREISISINRRVD---LAKAALYIATEDDSLVSHSSVP 122
           + +   +  +  + ++ +    E E  +++NR  D   L K    +  + D   + S   
Sbjct: 111 EISIQSKDSEISIAKVLFYIAAEDEAFLAVNRERDAQSLMKERESVQDQSDPSETDSEEL 170

Query: 123 LPIDA-----YVRRVNDLSMGYCTHYKS-SFNLSPENFLESIERYMYVMKGFRRTSSKTR 182
           L +D      +V  ++ +S        S          LE++   ++ ++GF+RTS    
Sbjct: 171 LQLDGKSISEWVSEIDAISKEVEAELVSRDIGCHLVQVLEAVNTVLFDLRGFKRTS--IT 230

Query: 183 SEPRALYLHTVLTHRTGSAALLSLIYSEILKMLR-------------FWSLLDFDVEIYH 242
            +P   YLH+VL  R  +A L+S+IY E+ K L               W   ++  E++ 
Sbjct: 231 LDPENSYLHSVLNCRCSTAFLISVIYIEVCKRLNVPIVGSPVGEDFLIWPKTEYPEELFK 290

Query: 243 PHDDYSL-----------PTGYHKQKSKESDQP-HIITTQSLLVEILSNLKESFWPFQQN 302
                SL           P       + +S Q   + T + ++   L+NL    W     
Sbjct: 291 ATSGQSLFSIVNGRCVDDPGSMASDLTAKSLQDLDMATNRDIIGIALANLIRLHWRRASK 350

Query: 303 QSRSLFLRAADAANCSDRSNVIEESGFQLASARAAQHRLERGVWTSVRFGDMRRALSACE 362
            S  L L +        + N I  S F L                 +R  D+R A++A E
Sbjct: 351 SSHGLMLTSP-----LSQLNNISSSNFPL-----------------LRPQDLRLAIAAAE 410

Query: 363 RLILLDVDSKEL-RDYSILLYHCGFYEQSLEYLKLYQEIKSSSSSPDALSCQEEEAVVN- 397
           RL++L   +  L RD  ++LY+   Y ++++ L +            A +  EEEAV+  
Sbjct: 411 RLLILQPHNWALRRDLGMMLYYDRQYGEAVQELSICM----------AFAPPEEEAVLEP 438

BLAST of Sgr016974.1 vs. TAIR 10
Match: AT4G19160.1 (unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 50.1 bits (118), Expect = 5.1e-06
Identity = 63/264 (23.86%), Postives = 118/264 (44.70%), Query Frame = 0

Query: 152 LESIERYMYVMKGFRRTSSKTRSEPRALYLHTVLTHRTGSAALLSLIYSEILKMLR---- 211
           LE++   ++ ++GF+RTS     +P   YLH+VL  R  +A L+S+IY E+ K L     
Sbjct: 60  LEAVNTVLFDLRGFKRTS--ITLDPENSYLHSVLNCRCSTAFLISVIYIEVCKRLNVPIV 119

Query: 212 ---------FWSLLDFDVEIYHPHDDYSLPTGYHKQKSKESDQP----HIITTQSLLVEI 271
                     W   ++  E++      SL   +     +  D P      +T +SL    
Sbjct: 120 GSPVGEDFLIWPKTEYPEELFKATSGQSL---FSIVNGRCVDDPGSMASDLTAKSLQDLD 179

Query: 272 LSNLKESFWPFQQNQSRSLFLRAADAANCSDRSNVIEESGFQLASARAAQHRLERGVWTS 331
           ++  ++       N  R  + RA+ +++           G  L S  +  + +    +  
Sbjct: 180 MATNRDIIGIALANLIRLHWRRASKSSH-----------GLMLTSPLSQLNNISSSNFPL 239

Query: 332 VRFGDMRRALSACERLILLDVDSKEL-RDYSILLYHCGFYEQSLEYLKLYQEIKSSSSSP 391
           +R  D+R A++A ERL++L   +  L RD  ++LY+   Y ++++ L +           
Sbjct: 240 LRPQDLRLAIAAAERLLILQPHNWALRRDLGMMLYYDRQYGEAVQELSICM--------- 297

Query: 392 DALSCQEEEAVVN-LMKRLTLIMM 397
            A +  EEEAV+   ++RL L+ +
Sbjct: 300 -AFAPPEEEAVLEPFVERLHLLRL 297

BLAST of Sgr016974.1 vs. TAIR 10
Match: AT4G19160.3 (unknown protein; Has 315 Blast hits to 315 proteins in 152 species: Archae - 0; Bacteria - 250; Metazoa - 2; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 17 (source: NCBI BLink). )

HSP 1 Score: 48.9 bits (115), Expect = 1.1e-05
Identity = 94/422 (22.27%), Postives = 174/422 (41.23%), Query Frame = 0

Query: 3   SFTAASASLWTPRLTTSSKFSKFNSSPPCFRVVCSGGFQQQYAAKDLRFLLHDAMDSFGI 62
           S+T     +  PR  T+S      S+ P FR   S   +     K+ + +   A   F  
Sbjct: 38  SWTGIEKKIPFPRKKTASA-----SAYPLFR---SQHTKDSSRPKNYKEVTKSARQMFAR 97

Query: 63  DSTHAKEARKGFLTQIQYLSNIEREISISINRRVD---LAKAALYIATEDDSLVSHSSVP 122
           + +   +  +  + ++ +    E E  +++NR  D   L K    +  + D   + S   
Sbjct: 98  EISIQSKDSEISIAKVLFYIAAEDEAFLAVNRERDAQSLMKERESVQDQSDPSETDSEEL 157

Query: 123 LPIDA-----YVRRVNDLSMGYCTHYKS-SFNLSPENFLESIERYMYVMKGFRRTSSKTR 182
           L +D      +V  ++ +S        S          LE++   ++ ++GF+RTS    
Sbjct: 158 LQLDGKSISEWVSEIDAISKEVEAELVSRDIGCHLVQVLEAVNTVLFDLRGFKRTS--IT 217

Query: 183 SEPRALYLHTVLTHRTGSAALLSLIYSEILKMLR-------------FWSLLDFDVEIYH 242
            +P   YLH+VL  R  +A L+S+IY E+ K L               W   ++  E++ 
Sbjct: 218 LDPENSYLHSVLNCRCSTAFLISVIYIEVCKRLNVPIVGSPVGEDFLIWPKTEYPEELFK 277

Query: 243 PHDDYSLPTGYHKQKSKESDQP----HIITTQSLLVEILSNLKESFWPFQQNQSRSLFLR 302
                SL   +     +  D P      +T +SL    ++  ++       N  R  + R
Sbjct: 278 ATSGQSL---FSIVNGRCVDDPGSMASDLTAKSLQDLDMATNRDIIGIALANLIRLHWRR 337

Query: 303 AADAANCSDRSNVIEESGFQLASARAAQHRLERGVWTSVRFGDMRRALSACERLILLDVD 362
           A+ +++           G  L S  +  + +    +  +R  D+R A++A ERL++L   
Sbjct: 338 ASKSSH-----------GLMLTSPLSQLNNISSSNFPLLRPQDLRLAIAAAERLLILQPH 397

Query: 363 SKEL-RDYSILLYHCGFYEQSLEYLKLYQEIKSSSSSPDALSCQEEEAVVN-LMKRLTLI 397
           +  L RD  ++LY+      S +Y +  QE+    S   A +  EEEAV+   ++RL L+
Sbjct: 398 NWALRRDLGMMLYY-----DSRQYGEAVQEL----SICMAFAPPEEEAVLEPFVERLHLL 426