Sgr016017.1 (mRNA) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr016017.1
Type	mRNA
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Pectin lyase-like superfamily protein
Location	tig00006406: 753989 .. 757352 (+)
Sequence length	1434
RNA-Seq Expression	Sgr016017.1
Synteny	Sgr016017.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATGATTCTGCTTGTTCTGGTGGTGACGTCGTCGCTGACGGAAGTTGAGAGCAGAAAGACGAGAAAGGTGAGAAATGCAAAGGCGAGGATTTTGGACGCCGATGGGTTTGAGTACGCCGCCATAAGTTGCAGAGCCCACAGTGCTTCTATAACTGATTTCGGCGGCGTTGGCGACGGAGAGACGTCGAATACCAAGGCGTTTCAGGAGGCAATTGGCCACCTCAGCCAATACGCCGGCGACGGCGGAGCTCAGCTGTATGTGCCGGCGGGGAGATGGCTCACCGGCAGCTTCAACCTCACCAGTCATTTCACTCTCTTTCTCCATCAGGATGCTGTCCTCCTTGCTTCTCAGGTTTCACCTTCTAACTCTCTCTCTCTCTTTTTGGTTTTTATATGTAAATGTTTTTTTTATAATTTTTTTTCTATGAATTTTGAGTCTATATTATTATCTTTTTTGGGTTAAATTTTCATATTGATCCCCTATACAAATTTTGCACGAGTGTAGGCTCCTATTTTAAATTATATATATTAAATTTGATGTCCATGGATGACCTAATATTCTTGTTACATGAAATATAAAAAGTTAATGGAACAAATTAATTTTTTATACAAATTTTTTTAAAAAGGTTAAGTACATATATATATATATATTTGTTATCACGAGTGACCTGTTACAAGATTCAATTTTATCAAAATATTTTTATTTGCAGCATCACGTGAATTGATAGCATCATGTTTGTTCGTATCCAAAATTCTAAAAAGAAATTGAACTAAAAAAAAAAATTATAAAAAGAAATTAAAACCAAATGTGTGTGTGTGTGTGTGTGTGTGTGTACATATATATAGAAAACAGGGAATGAAAAGATTTTGAATAAGGTTTAGAAAACAGGGAATGAGAAATGTAATAATTAAATCAAAATGACAGAAATACCCAAATGTTCTTTTCCTTTTACAAATCATTCCTTTTCATGTGAAGACAATTATAGCTGTTGAGAAAGTGAGTATTGAGTTACAAAACCACATAACGCTTTATGTAGAGTGGAATATTTTGGTAACTCCCAACCAGGTTTTTAGCAATTTTGACAGGAGAAACTGCCACTGTATTTACTTTCTTGTTACTTTTTTGTCTTTTTAGAACCTCTTTTCGGAATCTTGCCACGTGGACTATCAGTTGAAAAAACGACCTTGTAGTAGTGGTTTGAATTTGGGACCTCCAATTTTGATATCATGTTTAATCACTACTAAATTAAAAATGCTTAGGAGATGTTTTGGTAAGTTTAATGTTTTTCAATATATTCTCAATAAGATTGGCGGTGATCCAATGTCGTAGTTTTTGAAGAGATGAATAATTAAGTTTATTCAACCATATTCTCTCTTTTGCTTTCAGTGAAATTTTTTTCATGAATACTCAGATGACCGGTCATTATTATGGTTGCTGGAGCAACTTTAGTACTATCATTTTCTAGAGACATTTATTTAAAAAAAACACCTATAAGGAAGGTTGGATTAGGCCATTTAGAATCAATTTGTCATAATCAAAGTGATTTTCTTTTTGAATGTTTGGTTTTACAAAGTCATCAAGGCAGTTGAATTTAATAAATTGAAGTGATGAGTCCAAAGTGGGGGTAGTTTCTTTTCTCAGTGGTTTGAGAACAAACTACCAAAAATGGGTTGGGTCTGAAATCCATTGGATTCCATGTCAATTAGGTCCTAAAATCATGCCCTCTTCCATTTTCCAAGCCCTGGTTGTGGTTAGGTCAGCTAAAGTTGTTAAAAGCAAGAAAATGAGGATGTCGAACTAACTCAAACTCTTAAAGTCAAGGTTCACCCCCACAAAACGAAGCCTTCAGCCTACATTTGGCATTCATTTCATGTAGATATCCATATCGAGAAAATTTTTAATCAACTAAACTTGTCAATCGAGTCATCACTGTTAAATTGTCTAACTTTGTATCAAATGAATCAAATAAAAAATGATAGTCCTAATCTCAAGTATGCTTTTCAACTATTTTGATAGGATCCGAGTGAATGGCCTGTGGTCAAACCGTTGCCATCCTACGGCCATGGAAGGGACACATCAGGGGGAAGGTACATCAGTTTCATATTTGGAACAAACGTCACTGATGTTGTCATTACAGGTAAGTGTCTCAATGCCTCGCTGGAGCTGTGTCTGTATTGTCATTTTTCTTGATGAGTGTCTGAAACTTAGGGTGGAACCCGATTGATATTAGGGAACAATGGCACCATTGACGGTCAGGGTGCTCGCTGGTGGCAGCTGTTTCATCAGGGCAAGCTTAAGTACACCCGACCGTACTTGATCGAAATCATGTATTCAAGCGATGTACAGATATCGAACCTGACCCTGCTTAATTCCCCAGCTTGGAATGTTCATCCTGTTTACAGCAGGTAATCACAATCAGTTATTCCATGTAAAAACCTGTATAAAATTCAACACTTCCTGATTTGATTCCCTTGCAAATTTTATACAGCAATGTTCTTGTTCAAGGCATCACCATCATCGCTCCAGTTCGATCCCCTAACACAGATGGAATCAACCCAGGTTTGTATCTCGAAGCAACTCAGACTTCCTCGAGAAGACGTAGTTTTATACATCTTGTAATATCAATGACTGACTAACTTCATCTGCAGATTCCTGCACAAACGTTAGAATTGAGGATTGTTACATAGTATCTGGAGATGATTGTGTGGCTGTCAAGAGTGGCTGGGATGAGTATGGCATAGCATTCGGATGGCCGACTAAGCAATTGGTCATCAGACGGCTCTCATGCATTTCCCCGACAAGTGCTGTCATTGCACTGGGGAGTGAGATGTCCGGTGGGATCCAAGATGTCCGAGCCGAAGACATCGTAGCCGTTGATTCTGAATCGGGGGTCAGGATCAAAACTGGCATAGGAAGGGGAGGATATGTAAAAGACATATACGTAAGGAGGATGACAATGCATACCATGAAATGGGCATTTTGGATGACCGGCAATTATGGGTCACACGCCGATAGTAAGTACGACCCCCATGCGTTGCCTGTAATTCAAGGAATCAACTACAGGGATATGGTCGTCGAAAATGCAACAATGGCAGCGAGATTGGAGGGAATTTCGGGTGACACATTTACAGGTATCTGCATTTCCAATGTGACAATCAGCATGGCAGCAAAGGCCAAGAAACAGCCATGGACATGCACTGATGTTGAGGGAATAACAAGCCATGTTACTCCTCCACCGTGCAATCTGTTACCCGATCACGGGGAAGAAAAGATTACGGCATGCAAATTTCCAGAGGATAATCTACCAATTGATACAGTAGAGTTGAAAAAATGCTCTTTTCAATTGAGTCATGTTTGA

mRNA sequence

ATGGCGATGATTCTGCTTGTTCTGGTGGTGACGTCGTCGCTGACGGAAGTTGAGAGCAGAAAGACGAGAAAGGTGAGAAATGCAAAGGCGAGGATTTTGGACGCCGATGGGTTTGAGTACGCCGCCATAAGTTGCAGAGCCCACAGTGCTTCTATAACTGATTTCGGCGGCGTTGGCGACGGAGAGACGTCGAATACCAAGGCGTTTCAGGAGGCAATTGGCCACCTCAGCCAATACGCCGGCGACGGCGGAGCTCAGCTGTATGTGCCGGCGGGGAGATGGCTCACCGGCAGCTTCAACCTCACCAGTCATTTCACTCTCTTTCTCCATCAGGATGCTGTCCTCCTTGCTTCTCAGGATCCGAGTGAATGGCCTGTGGTCAAACCGTTGCCATCCTACGGCCATGGAAGGGACACATCAGGGGGAAGGTACATCAGTTTCATATTTGGAACAAACGTCACTGATGTTGTCATTACAGGGAACAATGGCACCATTGACGGTCAGGGTGCTCGCTGGTGGCAGCTGTTTCATCAGGGCAAGCTTAAGTACACCCGACCGTACTTGATCGAAATCATGTATTCAAGCGATGTACAGATATCGAACCTGACCCTGCTTAATTCCCCAGCTTGGAATGTTCATCCTGTTTACAGCAGCAATGTTCTTGTTCAAGGCATCACCATCATCGCTCCAGTTCGATCCCCTAACACAGATGGAATCAACCCAGATTCCTGCACAAACGTTAGAATTGAGGATTGTTACATAGTATCTGGAGATGATTGTGTGGCTGTCAAGAGTGGCTGGGATGAGTATGGCATAGCATTCGGATGGCCGACTAAGCAATTGGTCATCAGACGGCTCTCATGCATTTCCCCGACAAGTGCTGTCATTGCACTGGGGAGTGAGATGTCCGGTGGGATCCAAGATGTCCGAGCCGAAGACATCGTAGCCGTTGATTCTGAATCGGGGGTCAGGATCAAAACTGGCATAGGAAGGGGAGGATATGTAAAAGACATATACGTAAGGAGGATGACAATGCATACCATGAAATGGGCATTTTGGATGACCGGCAATTATGGGTCACACGCCGATAGTAAGTACGACCCCCATGCGTTGCCTGTAATTCAAGGAATCAACTACAGGGATATGGTCGTCGAAAATGCAACAATGGCAGCGAGATTGGAGGGAATTTCGGGTGACACATTTACAGGTATCTGCATTTCCAATGTGACAATCAGCATGGCAGCAAAGGCCAAGAAACAGCCATGGACATGCACTGATGTTGAGGGAATAACAAGCCATGTTACTCCTCCACCGTGCAATCTGTTACCCGATCACGGGGAAGAAAAGATTACGGCATGCAAATTTCCAGAGGATAATCTACCAATTGATACAGTAGAGTTGAAAAAATGCTCTTTTCAATTGAGTCATGTTTGA

Coding sequence (CDS)

ATGGCGATGATTCTGCTTGTTCTGGTGGTGACGTCGTCGCTGACGGAAGTTGAGAGCAGAAAGACGAGAAAGGTGAGAAATGCAAAGGCGAGGATTTTGGACGCCGATGGGTTTGAGTACGCCGCCATAAGTTGCAGAGCCCACAGTGCTTCTATAACTGATTTCGGCGGCGTTGGCGACGGAGAGACGTCGAATACCAAGGCGTTTCAGGAGGCAATTGGCCACCTCAGCCAATACGCCGGCGACGGCGGAGCTCAGCTGTATGTGCCGGCGGGGAGATGGCTCACCGGCAGCTTCAACCTCACCAGTCATTTCACTCTCTTTCTCCATCAGGATGCTGTCCTCCTTGCTTCTCAGGATCCGAGTGAATGGCCTGTGGTCAAACCGTTGCCATCCTACGGCCATGGAAGGGACACATCAGGGGGAAGGTACATCAGTTTCATATTTGGAACAAACGTCACTGATGTTGTCATTACAGGGAACAATGGCACCATTGACGGTCAGGGTGCTCGCTGGTGGCAGCTGTTTCATCAGGGCAAGCTTAAGTACACCCGACCGTACTTGATCGAAATCATGTATTCAAGCGATGTACAGATATCGAACCTGACCCTGCTTAATTCCCCAGCTTGGAATGTTCATCCTGTTTACAGCAGCAATGTTCTTGTTCAAGGCATCACCATCATCGCTCCAGTTCGATCCCCTAACACAGATGGAATCAACCCAGATTCCTGCACAAACGTTAGAATTGAGGATTGTTACATAGTATCTGGAGATGATTGTGTGGCTGTCAAGAGTGGCTGGGATGAGTATGGCATAGCATTCGGATGGCCGACTAAGCAATTGGTCATCAGACGGCTCTCATGCATTTCCCCGACAAGTGCTGTCATTGCACTGGGGAGTGAGATGTCCGGTGGGATCCAAGATGTCCGAGCCGAAGACATCGTAGCCGTTGATTCTGAATCGGGGGTCAGGATCAAAACTGGCATAGGAAGGGGAGGATATGTAAAAGACATATACGTAAGGAGGATGACAATGCATACCATGAAATGGGCATTTTGGATGACCGGCAATTATGGGTCACACGCCGATAGTAAGTACGACCCCCATGCGTTGCCTGTAATTCAAGGAATCAACTACAGGGATATGGTCGTCGAAAATGCAACAATGGCAGCGAGATTGGAGGGAATTTCGGGTGACACATTTACAGGTATCTGCATTTCCAATGTGACAATCAGCATGGCAGCAAAGGCCAAGAAACAGCCATGGACATGCACTGATGTTGAGGGAATAACAAGCCATGTTACTCCTCCACCGTGCAATCTGTTACCCGATCACGGGGAAGAAAAGATTACGGCATGCAAATTTCCAGAGGATAATCTACCAATTGATACAGTAGAGTTGAAAAAATGCTCTTTTCAATTGAGTCATGTTTGA

Protein sequence

MAMILLVLVVTSSLTEVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVGDGETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDPSEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALGSEMSGGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSHADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKKQPWTCTDVEGITSHVTPPPCNLLPDHGEEKITACKFPEDNLPIDTVELKKCSFQLSHV

Homology

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BLAST of Sgr016017.1 vs. NCBI nr
Match: XP_022147419.1 (probable polygalacturonase [Momordica charantia])

HSP 1 Score: 866.3 bits (2237), Expect = 1.2e-247
Identity = 416/476 (87.39%), Postives = 445/476 (93.49%), Query Frame = 0


Query: 3   MILLVLVVTSSLT-EVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVGDG 62
           M + V+V+ ++L  EVE R+ +K   A+ R LD DGFEY AISCRAHSASIT+FGGVGDG
Sbjct: 16  MAVFVVVLAAALAGEVEGRRGKK---ARRRALDGDGFEYGAISCRAHSASITEFGGVGDG 75

Query: 63  ETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDP 122
            TSNTKAFQ A+  LSQYAG+GGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDP
Sbjct: 76  VTSNTKAFQAAVERLSQYAGEGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDP 135

Query: 123 SEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQGKL 182
           +EWPVV PLPSYGHGRDTSGGRYIS +FGTN+TDVVITGNNGTIDGQGARWWQLFHQGKL
Sbjct: 136 NEWPVVDPLPSYGHGRDTSGGRYISLLFGTNLTDVVITGNNGTIDGQGARWWQLFHQGKL 195

Query: 183 KYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGINP 242
           KYTRPYLIEIM+S+DVQISNLTLLN+P WNVHPVYSSNVL+QGITIIAPVRSPNTDGINP
Sbjct: 196 KYTRPYLIEIMHSNDVQISNLTLLNAPTWNVHPVYSSNVLIQGITIIAPVRSPNTDGINP 255

Query: 243 DSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALGSE 302
           DSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQL+IRRL+CISPTSAVIALGSE
Sbjct: 256 DSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLIIRRLTCISPTSAVIALGSE 315

Query: 303 MSGGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSH 362
           MSGGIQDVRAEDIVA+DSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSH
Sbjct: 316 MSGGIQDVRAEDIVAIDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSH 375

Query: 363 ADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKKQP 422
           AD KYDPHA+P I+GINYRDMVVENATMAARLEGISGDTF GICISNVTI MA KAKKQP
Sbjct: 376 ADKKYDPHAMPAIEGINYRDMVVENATMAARLEGISGDTFKGICISNVTIGMAVKAKKQP 435

Query: 423 WTCTDVEGITSHVTPPPCNLLPDHGEEKITACKFPEDNLPIDTVELKKCSFQLSHV 478
           WTCTD+EGITSHVTPPPC+LLPD GE+  T C+FPED LPID+VELKKCS+QLSHV
Sbjct: 436 WTCTDIEGITSHVTPPPCSLLPDQGEQ-TTTCQFPEDTLPIDSVELKKCSYQLSHV 487

[Close]

BLAST of Sgr016017.1 vs. NCBI nr
Match: XP_022974569.1 (probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 865.1 bits (2234), Expect = 2.7e-247
Identity = 417/475 (87.79%), Postives = 443/475 (93.26%), Query Frame = 0


Query: 3   MILLVLVVTSSLT-EVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVGDG 62
           M  LVLV+ ++L  EVE R        KARIL+ +G EY AISCRAH+ASIT+FGGVGDG
Sbjct: 18  MTFLVLVLAAALMGEVEGR--------KARILEDNGIEYGAISCRAHTASITEFGGVGDG 77

Query: 63  ETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDP 122
           ETSNTKAFQ A+  LSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDP
Sbjct: 78  ETSNTKAFQAAVERLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDP 137

Query: 123 SEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQGKL 182
           +EWPVV+PLPSYGHGRDTSGGRYIS IFGTN+TDVVITGNNGTIDGQG RWWQLFHQ KL
Sbjct: 138 NEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDGQGDRWWQLFHQNKL 197

Query: 183 KYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGINP 242
           KYTRPYLIEIMYSS++QISNLTLLNSPAWNVHPVYSSNVL+QGITIIAPVRSPNTDGINP
Sbjct: 198 KYTRPYLIEIMYSSNIQISNLTLLNSPAWNVHPVYSSNVLIQGITIIAPVRSPNTDGINP 257

Query: 243 DSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALGSE 302
           DSCTNVRIEDCYI+SGDDCVAVKSGWDEYGI FGWPTKQLVIRRL+C+SPTSAVIALGSE
Sbjct: 258 DSCTNVRIEDCYIISGDDCVAVKSGWDEYGIRFGWPTKQLVIRRLTCVSPTSAVIALGSE 317

Query: 303 MSGGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSH 362
           MSGGI+DVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTG+YGSH
Sbjct: 318 MSGGIEDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGDYGSH 377

Query: 363 ADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKKQP 422
           AD  YDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTIS+AAKAKKQP
Sbjct: 378 ADENYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISLAAKAKKQP 437

Query: 423 WTCTDVEGITSHVTPPPCNLLPDHGEEKITACKFPEDNLPIDTVELKKCSFQLSH 477
           WTCTDVEG TS+VTPPPC+LLPD G EK T C+FPE+N+PID VELKKCS+QLSH
Sbjct: 438 WTCTDVEGTTSNVTPPPCSLLPDQGAEKFTTCRFPEENIPIDAVELKKCSYQLSH 484

[Close]

BLAST of Sgr016017.1 vs. NCBI nr
Match: XP_022935413.1 (probable polygalacturonase [Cucurbita moschata])

HSP 1 Score: 861.7 bits (2225), Expect = 3.0e-246
Identity = 416/477 (87.21%), Postives = 442/477 (92.66%), Query Frame = 0


Query: 1   MAMILLVLVVTSSLT-EVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVG 60
           M  +++VL   ++L  EVE R        K RIL+ +G EY AISCRAH+ASIT+FGGVG
Sbjct: 18  MTFLVVVLAAAAALMGEVEGR--------KVRILEDNGIEYGAISCRAHTASITEFGGVG 77

Query: 61  DGETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQ 120
           DGETSNTKAFQ A+  LSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQ
Sbjct: 78  DGETSNTKAFQAAVERLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQ 137

Query: 121 DPSEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQG 180
           D +EWPVV+PLPSYGHGRDTSGGRYIS IFGTN+TDVVITGNNGTIDGQG RWWQLFHQ 
Sbjct: 138 DLNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDGQGDRWWQLFHQN 197

Query: 181 KLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGI 240
           KLKYTRPYLIEIMYSS+VQISNLTLLNSPAWNVHPVYSSNVL+QGITIIAPVRSPNTDGI
Sbjct: 198 KLKYTRPYLIEIMYSSNVQISNLTLLNSPAWNVHPVYSSNVLIQGITIIAPVRSPNTDGI 257

Query: 241 NPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALG 300
           NPDSCTNVRIEDCYI+SGDDCVAVKSGWDEYGI FGWPTKQLVIRRL+C+SPTSAVIALG
Sbjct: 258 NPDSCTNVRIEDCYIISGDDCVAVKSGWDEYGIRFGWPTKQLVIRRLTCVSPTSAVIALG 317

Query: 301 SEMSGGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYG 360
           SEMSGGI+DVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTG+YG
Sbjct: 318 SEMSGGIEDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGDYG 377

Query: 361 SHADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKK 420
           SHAD  YDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTIS+AAKAKK
Sbjct: 378 SHADENYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISLAAKAKK 437

Query: 421 QPWTCTDVEGITSHVTPPPCNLLPDHGEEKITACKFPEDNLPIDTVELKKCSFQLSH 477
           QPWTCTDVEGITS+VTPPPC+LLPD G EKIT C+FPEDN+PID VELKKCS+Q SH
Sbjct: 438 QPWTCTDVEGITSNVTPPPCSLLPDQGAEKITTCRFPEDNIPIDAVELKKCSYQFSH 486

[Close]

BLAST of Sgr016017.1 vs. NCBI nr
Match: XP_004133855.1 (probable polygalacturonase [Cucumis sativus] >KGN56496.1 hypothetical protein Csa_011241 [Cucumis sativus])

HSP 1 Score: 860.9 bits (2223), Expect = 5.1e-246
Identity = 409/477 (85.74%), Postives = 444/477 (93.08%), Query Frame = 0


Query: 1   MAMILL-VLVVTSSLTEVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVG 60
           M M++L V+V  + + EVE RK +  +  KA+IL+ +G EY AISCRAH+ASIT+FGGVG
Sbjct: 16  MKMVILGVIVAAALMDEVEGRKGKVKKGKKAKILEGNGIEYGAISCRAHTASITEFGGVG 75

Query: 61  DGETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQ 120
           DG TSNTKAFQ A+  LSQY  DGGAQLYVPAG+WLTGSFNLTSHFTLFLHQDAVLLASQ
Sbjct: 76  DGVTSNTKAFQAAVEQLSQYTDDGGAQLYVPAGKWLTGSFNLTSHFTLFLHQDAVLLASQ 135

Query: 121 DPSEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQG 180
           DP+EWPVV+PLPSYGHGRDTSGGRYIS IFGTN+TDVVITGNNGTIDGQG RWWQLFHQG
Sbjct: 136 DPNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDGQGDRWWQLFHQG 195

Query: 181 KLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGI 240
           KLKYTRPYLIE+MYSSD+QIS+LTLLNSPAWNVHPVYSSN+L+QGITIIAPVRSPNTDGI
Sbjct: 196 KLKYTRPYLIELMYSSDIQISSLTLLNSPAWNVHPVYSSNILIQGITIIAPVRSPNTDGI 255

Query: 241 NPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALG 300
           NPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGI+FG PTKQLV+RRL+CISPTSAVIALG
Sbjct: 256 NPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGISFGLPTKQLVVRRLTCISPTSAVIALG 315

Query: 301 SEMSGGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYG 360
           SEMSGGI+DVRAEDIVA+DSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTG+YG
Sbjct: 316 SEMSGGIEDVRAEDIVAIDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGDYG 375

Query: 361 SHADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKK 420
           SHAD  YDPHALPVIQGINY+DMVVENATMAARLEGISGDTFT ICISNVTI +A KAKK
Sbjct: 376 SHADKNYDPHALPVIQGINYKDMVVENATMAARLEGISGDTFTDICISNVTIGLAPKAKK 435

Query: 421 QPWTCTDVEGITSHVTPPPCNLLPDHGEEKITACKFPEDNLPIDTVELKKCSFQLSH 477
           QPWTCTD+EGITS VTPPPC+LLPD GEEKIT CKFPEDNLPID+V+LKKCS+Q SH
Sbjct: 436 QPWTCTDIEGITSQVTPPPCDLLPDQGEEKITTCKFPEDNLPIDSVQLKKCSYQFSH 492

[Close]

BLAST of Sgr016017.1 vs. NCBI nr
Match: XP_023526703.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 860.5 bits (2222), Expect = 6.7e-246
Identity = 413/474 (87.13%), Postives = 442/474 (93.25%), Query Frame = 0


Query: 4   ILLVLVVTSSLTEVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVGDGET 63
           IL++++  + + EVE RK RK R  K RI++    EY AISCRAHSASITDFGGVGDGET
Sbjct: 18  ILVLILAAAMMGEVEGRKGRKHR--KGRIVEGKMIEYGAISCRAHSASITDFGGVGDGET 77

Query: 64  SNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDPSE 123
           SNTKAFQ A+  LSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQD +E
Sbjct: 78  SNTKAFQAAVERLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDLNE 137

Query: 124 WPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQGKLKY 183
           W VV+PLPSYGHGRDTSGGRYIS IFGTN+TDVVITGNNGTIDGQG RWWQLFHQGKLKY
Sbjct: 138 WSVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDGQGDRWWQLFHQGKLKY 197

Query: 184 TRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGINPDS 243
           TRPYLIEIMYSS++QISNLTLLNSP+WNVHPVYSSN+++QGITIIAPVRSPNTDGINPDS
Sbjct: 198 TRPYLIEIMYSSNIQISNLTLLNSPSWNVHPVYSSNLVIQGITIIAPVRSPNTDGINPDS 257

Query: 244 CTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALGSEMS 303
           CTNVRIEDCYIVSGDDCVAVKSGWDEYGI FGWPTKQLVIRRL+CISPTSAVIALGSEMS
Sbjct: 258 CTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLVIRRLTCISPTSAVIALGSEMS 317

Query: 304 GGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSHAD 363
           GGIQDVRAEDI A++SESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSHAD
Sbjct: 318 GGIQDVRAEDITAINSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSHAD 377

Query: 364 SKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKKQPWT 423
            KYDPHALPVIQGINYRD+VVENATMAARLEGISGD+FT ICISNVTISMA KAKKQPWT
Sbjct: 378 KKYDPHALPVIQGINYRDIVVENATMAARLEGISGDSFTDICISNVTISMAPKAKKQPWT 437

Query: 424 CTDVEGITSHVTPPPCNLLPDHGEEKITACKFPEDNLPIDTVELKKCSFQLSHV 478
           CTDVEGITS VTPPPC+LLPD GEEKIT CKFPED +PI+ VEL+KCS+Q+SHV
Sbjct: 438 CTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDTIPIEAVELRKCSYQISHV 489

[Close]

BLAST of Sgr016017.1 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 5.0e-220
Identity = 365/473 (77.17%), Postives = 413/473 (87.32%), Query Frame = 0


Query: 4   ILLVLVVTSSLTEVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVGDGET 63
           +L+ +V+  S++  E R        K RIL+A   EY+AISCRAHSAS+ DFGGVGDG+T
Sbjct: 27  VLVAVVLLLSVSRGECR--------KGRILEA--LEYSAISCRAHSASLVDFGGVGDGQT 86

Query: 64  SNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDPSE 123
            NTKAFQ+A+  LS+Y  +GGAQLYVPAG+WLTGSF+LTSHFTLFLH+DAVLLASQD S+
Sbjct: 87  LNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLHRDAVLLASQDISQ 146

Query: 124 WPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQGKLKY 183
           WPV+KPLPSYG GRD + GRY S IFGTN+TDV+ITG+NGTIDGQG  WWQ FH GKLKY
Sbjct: 147 WPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGGLWWQRFHGGKLKY 206

Query: 184 TRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGINPDS 243
           TRPYLIE+MYS+D+QISNLTLLNSP+WNVHPVYS N+L+QGITI+APVRSPNTDGINPDS
Sbjct: 207 TRPYLIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGINPDS 266

Query: 244 CTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALGSEMS 303
           CTN RIEDCYIVSGDDCVAVKSGWDEYGIA+G PTKQLVIRRL+CISP SAVIALGSEMS
Sbjct: 267 CTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMS 326

Query: 304 GGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSHAD 363
           GGIQDVRAEDIVA++SESG+RIKTGIGRGGYVKDIYVR MTM TMKWAFWMTGNYGSHAD
Sbjct: 327 GGIQDVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKWAFWMTGNYGSHAD 386

Query: 364 SKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKKQPWT 423
           + YDP A PVIQGINYRDMV EN +MAARLEGI  D FTGICISNVTI +AAKAKK PWT
Sbjct: 387 NHYDPKAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVTIHLAAKAKKVPWT 446

Query: 424 CTDVEGITSHVTPPPCNLLPDHGEEKITACKFPEDNLPIDTVELKKCSFQLSH 477
           CTDVEGI+S VTP PC+ LPD G EK + C FP ++LPIDTVEL+KCS+ +++
Sbjct: 447 CTDVEGISSGVTPTPCSTLPDQGPEKTSLCNFPAESLPIDTVELQKCSYGINY 489

[Close]

BLAST of Sgr016017.1 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.7e-26
Identity = 117/413 (28.33%), Postives = 183/413 (44.31%), Query Frame = 0


Query: 51  SITDFGGVGDGETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGS---FNLTSHFTL 110
           ++  +G VGDG+  +T+AF        + A    A L VPA +    +   F       L
Sbjct: 60  NVEQYGAVGDGKHDSTEAFATTWNAACKKA---SAVLLVPANKKFFVNNLVFRGPCQPHL 119

Query: 111 FLHQDAVLLASQDPSEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDG 170
               D  ++A  DP+ W   K               ++ F     +TD  + G  G IDG
Sbjct: 120 SFKVDGTIVAQPDPARWKNSK--------------IWLQF---AQLTDFNLMG-TGVIDG 179

Query: 171 QGARWWQLFHQGKLKY----------TRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYS 230
           QG +WW     G+ K            RP  I+I YS  V +  LTL+NSP +++     
Sbjct: 180 QGQQWW----AGQCKVVNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGEC 239

Query: 231 SNVLVQGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWP 290
             V +QG+ I AP  SPNTDGI+  +     IE C I +GDDC+A+ +G           
Sbjct: 240 EGVKIQGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTG----------- 299

Query: 291 TKQLVIRRLSCISPTSAVI-ALGSEMS-GGIQDVRAEDIVAVDSESGVRIKTGIGRGGYV 350
           +  + I+ L C       I +LG + S   +  V       +D+++G+RIKT  G  G  
Sbjct: 300 SSNITIKDLICGPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLA 359

Query: 351 KDIYVRRMTMHTMKWAFWMTGNYGSHADSKYDPHALPVIQGINYRDMVVENATMAARLEG 410
             I    + M   +    +   Y + A +  +  +   IQG+ Y+++   +AT AA ++ 
Sbjct: 360 SYITYENVEMINSENPILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTSAT-AAAIQL 419

Query: 411 ISGDTF--TGICISNVTISMAAKAKKQPWTCTD--VEGITSHVTPPPC-NLLP 444
           +  D+   TGI +SNV++ + +    +P +C D    G  S    P C NL P
Sbjct: 420 MCSDSVPCTGIQLSNVSLKLTS---GKPASCVDKNARGFYSGRLIPTCKNLRP 432

[Close]

BLAST of Sgr016017.1 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.2e-24
Identity = 97/321 (30.22%), Postives = 157/321 (48.91%), Query Frame = 0


Query: 40  YAAISCRAHSASITDFGGVGDGETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRW----- 99
           +  I   +++  +T FG VGDG T +++AF +A   +    GDG  Q  VPAG       
Sbjct: 14  FLQIFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDG--QFVVPAGMTFMLQP 73

Query: 100 --LTGSFNLTSHFTLFLHQDAVLLASQDPSEWPVVKPLPSYGHGRDTSGGRYISFIFGTN 159
               GS   T  F   L +   L+A             PS G+ +    G    +I  T+
Sbjct: 74  LKFQGSCKSTPVFVQMLGK---LVA-------------PSKGNWK----GDKDQWILFTD 133

Query: 160 VTDVVITGNNGTIDGQGARWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNV 219
           +  +VI G +G I+GQG+ WW+  H+G    +RP  ++    +++++S LT L+SP  ++
Sbjct: 134 IEGLVIEG-DGEINGQGSSWWE--HKG----SRPTALKFRSCNNLRLSGLTHLDSPMAHI 193

Query: 220 HPVYSSNVLVQGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGI 279
           H    + V +  + I AP  SPNTDGI+  + +NV I+DC I +GDDC+A+ SG      
Sbjct: 194 HISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG------ 253

Query: 280 AFGWPTKQLVIRRLSCISPTSAVIALGSEMSGG----IQDVRAEDIVAVDSESGVRIKTG 339
                T  + I  + C  P    I++GS    G    +++V  ++     + +G RIKT 
Sbjct: 254 -----TSNIHISGIDC-GPGHG-ISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTW 292

Query: 340 IGRGGYVKDIYVRRMTMHTMK 350
            G  GY + I    +T+  ++
Sbjct: 314 QGGSGYARMITFNGITLDNVE 292

[Close]

BLAST of Sgr016017.1 vs. ExPASy Swiss-Prot
Match: P27644 (Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 4.7e-24
Identity = 70/210 (33.33%), Postives = 105/210 (50.00%), Query Frame = 0


Query: 198 QISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSG 257
           Q+   T+ N+ +W +HP    ++     TIIAP  SPNTDG NP+SC NV I       G
Sbjct: 34  QLLGFTIRNAASWTIHPQGCEDLTAAASTIIAPHDSPNTDGFNPESCRNVMISGVRFSVG 93

Query: 258 DDCVAVKSG-----WDEYGIAFGWPTKQLVIRRLSCISPTSAVIALGSEMSGGIQDVRAE 317
           DDC+AVK+G      ++  +A    T+ + +R    + P    + +GSEMSGG+ DV  E
Sbjct: 94  DDCIAVKAGKRGPDGEDDHLA---ETRGITVRH-CLMQPGHGGLVIGSEMSGGVHDVTVE 153

Query: 318 DIVAVDSESGVRIKTGI-GRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSHADSKYD---- 377
           D   + ++ G+R+KTG    GG V +I +RR+ +  ++ A     +Y   AD   D    
Sbjct: 154 DCDMIGTDRGLRLKTGARSGGGMVGNITMRRVLLDGVQTALSANAHYHCDADGHDDWVQS 213

Query: 378 ------PHALPVIQGINYRDMVVENATMAA 392
                     P + GI   D+ + N   AA
Sbjct: 214 RNPAPVNDGTPFVDGITVEDVEIRNLAHAA 239

[Close]

BLAST of Sgr016017.1 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 3.9e-23
Identity = 108/401 (26.93%), Postives = 178/401 (44.39%), Query Frame = 0


Query: 49  SASITDFGGVGDGETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGR-WLTGSFNLTS--HF 108
           + S++DFG  GDG+T +T+AF  A       + +G   L VP G  +L  S  LT   + 
Sbjct: 67  TVSVSDFGAKGDGKTDDTQAFVNA--WKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNS 126

Query: 109 TLFLHQDAVLLASQDPSEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTI 168
            L +     L ASQ  S++            +D S  ++I F    N++  V  G+ G +
Sbjct: 127 ILTVQIFGTLSASQKRSDY------------KDIS--KWIMFDGVNNLS--VDGGDTGVV 186

Query: 169 DGQGARWWQ---LFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLV 228
           DG G  WWQ     ++ K     P  +    S  + + NL + N+    +     SNV V
Sbjct: 187 DGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQV 246

Query: 229 QGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLV 288
             + + AP  SPNTDGI+  +  N+R+ +  I +GDDC++++SG           ++ + 
Sbjct: 247 SNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------SQNVQ 306

Query: 289 IRRLSCISPTSAVI-ALGSEMSGG-IQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYV 348
           I  ++C       I +LG + S   +  V  +      +++GVRIKT  G  G   +I  
Sbjct: 307 INDITCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIF 366

Query: 349 RRMTMHTMKWAFWMTGNYGSHADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGD- 408
           + + M  +K    +  +Y   +    +  A+ V + + YRD+   +A+  A     S + 
Sbjct: 367 QNIQMDNVKNPIIIDQDYCDKSKCTTEKSAVQV-KNVVYRDISGTSASENAITFNCSKNY 426

Query: 409 TFTGICISNVTISMAAKAKKQPWTCTDVEGITSHVTPPPCN 441
              GI +  V I    KA     TCT+   +      P CN
Sbjct: 427 PCQGIVLDRVNIK-GGKA-----TCTNANVVDKGAVLPQCN 431

[Close]

BLAST of Sgr016017.1 vs. ExPASy TrEMBL
Match: A0A6J1D0Y3 (probable polygalacturonase OS=Momordica charantia OX=3673 GN=LOC111016350 PE=3 SV=1)

HSP 1 Score: 866.3 bits (2237), Expect = 5.9e-248
Identity = 416/476 (87.39%), Postives = 445/476 (93.49%), Query Frame = 0


Query: 3   MILLVLVVTSSLT-EVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVGDG 62
           M + V+V+ ++L  EVE R+ +K   A+ R LD DGFEY AISCRAHSASIT+FGGVGDG
Sbjct: 16  MAVFVVVLAAALAGEVEGRRGKK---ARRRALDGDGFEYGAISCRAHSASITEFGGVGDG 75

Query: 63  ETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDP 122
            TSNTKAFQ A+  LSQYAG+GGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDP
Sbjct: 76  VTSNTKAFQAAVERLSQYAGEGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDP 135

Query: 123 SEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQGKL 182
           +EWPVV PLPSYGHGRDTSGGRYIS +FGTN+TDVVITGNNGTIDGQGARWWQLFHQGKL
Sbjct: 136 NEWPVVDPLPSYGHGRDTSGGRYISLLFGTNLTDVVITGNNGTIDGQGARWWQLFHQGKL 195

Query: 183 KYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGINP 242
           KYTRPYLIEIM+S+DVQISNLTLLN+P WNVHPVYSSNVL+QGITIIAPVRSPNTDGINP
Sbjct: 196 KYTRPYLIEIMHSNDVQISNLTLLNAPTWNVHPVYSSNVLIQGITIIAPVRSPNTDGINP 255

Query: 243 DSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALGSE 302
           DSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQL+IRRL+CISPTSAVIALGSE
Sbjct: 256 DSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLIIRRLTCISPTSAVIALGSE 315

Query: 303 MSGGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSH 362
           MSGGIQDVRAEDIVA+DSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSH
Sbjct: 316 MSGGIQDVRAEDIVAIDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSH 375

Query: 363 ADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKKQP 422
           AD KYDPHA+P I+GINYRDMVVENATMAARLEGISGDTF GICISNVTI MA KAKKQP
Sbjct: 376 ADKKYDPHAMPAIEGINYRDMVVENATMAARLEGISGDTFKGICISNVTIGMAVKAKKQP 435

Query: 423 WTCTDVEGITSHVTPPPCNLLPDHGEEKITACKFPEDNLPIDTVELKKCSFQLSHV 478
           WTCTD+EGITSHVTPPPC+LLPD GE+  T C+FPED LPID+VELKKCS+QLSHV
Sbjct: 436 WTCTDIEGITSHVTPPPCSLLPDQGEQ-TTTCQFPEDTLPIDSVELKKCSYQLSHV 487

[Close]

BLAST of Sgr016017.1 vs. ExPASy TrEMBL
Match: A0A6J1II01 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111473265 PE=3 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.3e-247
Identity = 417/475 (87.79%), Postives = 443/475 (93.26%), Query Frame = 0


Query: 3   MILLVLVVTSSLT-EVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVGDG 62
           M  LVLV+ ++L  EVE R        KARIL+ +G EY AISCRAH+ASIT+FGGVGDG
Sbjct: 18  MTFLVLVLAAALMGEVEGR--------KARILEDNGIEYGAISCRAHTASITEFGGVGDG 77

Query: 63  ETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDP 122
           ETSNTKAFQ A+  LSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDP
Sbjct: 78  ETSNTKAFQAAVERLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDP 137

Query: 123 SEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQGKL 182
           +EWPVV+PLPSYGHGRDTSGGRYIS IFGTN+TDVVITGNNGTIDGQG RWWQLFHQ KL
Sbjct: 138 NEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDGQGDRWWQLFHQNKL 197

Query: 183 KYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGINP 242
           KYTRPYLIEIMYSS++QISNLTLLNSPAWNVHPVYSSNVL+QGITIIAPVRSPNTDGINP
Sbjct: 198 KYTRPYLIEIMYSSNIQISNLTLLNSPAWNVHPVYSSNVLIQGITIIAPVRSPNTDGINP 257

Query: 243 DSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALGSE 302
           DSCTNVRIEDCYI+SGDDCVAVKSGWDEYGI FGWPTKQLVIRRL+C+SPTSAVIALGSE
Sbjct: 258 DSCTNVRIEDCYIISGDDCVAVKSGWDEYGIRFGWPTKQLVIRRLTCVSPTSAVIALGSE 317

Query: 303 MSGGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSH 362
           MSGGI+DVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTG+YGSH
Sbjct: 318 MSGGIEDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGDYGSH 377

Query: 363 ADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKKQP 422
           AD  YDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTIS+AAKAKKQP
Sbjct: 378 ADENYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISLAAKAKKQP 437

Query: 423 WTCTDVEGITSHVTPPPCNLLPDHGEEKITACKFPEDNLPIDTVELKKCSFQLSH 477
           WTCTDVEG TS+VTPPPC+LLPD G EK T C+FPE+N+PID VELKKCS+QLSH
Sbjct: 438 WTCTDVEGTTSNVTPPPCSLLPDQGAEKFTTCRFPEENIPIDAVELKKCSYQLSH 484

[Close]

BLAST of Sgr016017.1 vs. ExPASy TrEMBL
Match: A0A6J1FAH2 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111442298 PE=3 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.5e-246
Identity = 416/477 (87.21%), Postives = 442/477 (92.66%), Query Frame = 0


Query: 1   MAMILLVLVVTSSLT-EVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVG 60
           M  +++VL   ++L  EVE R        K RIL+ +G EY AISCRAH+ASIT+FGGVG
Sbjct: 18  MTFLVVVLAAAAALMGEVEGR--------KVRILEDNGIEYGAISCRAHTASITEFGGVG 77

Query: 61  DGETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQ 120
           DGETSNTKAFQ A+  LSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQ
Sbjct: 78  DGETSNTKAFQAAVERLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQ 137

Query: 121 DPSEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQG 180
           D +EWPVV+PLPSYGHGRDTSGGRYIS IFGTN+TDVVITGNNGTIDGQG RWWQLFHQ 
Sbjct: 138 DLNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDGQGDRWWQLFHQN 197

Query: 181 KLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGI 240
           KLKYTRPYLIEIMYSS+VQISNLTLLNSPAWNVHPVYSSNVL+QGITIIAPVRSPNTDGI
Sbjct: 198 KLKYTRPYLIEIMYSSNVQISNLTLLNSPAWNVHPVYSSNVLIQGITIIAPVRSPNTDGI 257

Query: 241 NPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALG 300
           NPDSCTNVRIEDCYI+SGDDCVAVKSGWDEYGI FGWPTKQLVIRRL+C+SPTSAVIALG
Sbjct: 258 NPDSCTNVRIEDCYIISGDDCVAVKSGWDEYGIRFGWPTKQLVIRRLTCVSPTSAVIALG 317

Query: 301 SEMSGGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYG 360
           SEMSGGI+DVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTG+YG
Sbjct: 318 SEMSGGIEDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGDYG 377

Query: 361 SHADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKK 420
           SHAD  YDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTIS+AAKAKK
Sbjct: 378 SHADENYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISLAAKAKK 437

Query: 421 QPWTCTDVEGITSHVTPPPCNLLPDHGEEKITACKFPEDNLPIDTVELKKCSFQLSH 477
           QPWTCTDVEGITS+VTPPPC+LLPD G EKIT C+FPEDN+PID VELKKCS+Q SH
Sbjct: 438 QPWTCTDVEGITSNVTPPPCSLLPDQGAEKITTCRFPEDNIPIDAVELKKCSYQFSH 486

[Close]

BLAST of Sgr016017.1 vs. ExPASy TrEMBL
Match: A0A0A0L8V4 (Pectate_lyase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G121720 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.5e-246
Identity = 409/477 (85.74%), Postives = 444/477 (93.08%), Query Frame = 0


Query: 1   MAMILL-VLVVTSSLTEVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVG 60
           M M++L V+V  + + EVE RK +  +  KA+IL+ +G EY AISCRAH+ASIT+FGGVG
Sbjct: 16  MKMVILGVIVAAALMDEVEGRKGKVKKGKKAKILEGNGIEYGAISCRAHTASITEFGGVG 75

Query: 61  DGETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQ 120
           DG TSNTKAFQ A+  LSQY  DGGAQLYVPAG+WLTGSFNLTSHFTLFLHQDAVLLASQ
Sbjct: 76  DGVTSNTKAFQAAVEQLSQYTDDGGAQLYVPAGKWLTGSFNLTSHFTLFLHQDAVLLASQ 135

Query: 121 DPSEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQG 180
           DP+EWPVV+PLPSYGHGRDTSGGRYIS IFGTN+TDVVITGNNGTIDGQG RWWQLFHQG
Sbjct: 136 DPNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDGQGDRWWQLFHQG 195

Query: 181 KLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGI 240
           KLKYTRPYLIE+MYSSD+QIS+LTLLNSPAWNVHPVYSSN+L+QGITIIAPVRSPNTDGI
Sbjct: 196 KLKYTRPYLIELMYSSDIQISSLTLLNSPAWNVHPVYSSNILIQGITIIAPVRSPNTDGI 255

Query: 241 NPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALG 300
           NPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGI+FG PTKQLV+RRL+CISPTSAVIALG
Sbjct: 256 NPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGISFGLPTKQLVVRRLTCISPTSAVIALG 315

Query: 301 SEMSGGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYG 360
           SEMSGGI+DVRAEDIVA+DSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTG+YG
Sbjct: 316 SEMSGGIEDVRAEDIVAIDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGDYG 375

Query: 361 SHADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKK 420
           SHAD  YDPHALPVIQGINY+DMVVENATMAARLEGISGDTFT ICISNVTI +A KAKK
Sbjct: 376 SHADKNYDPHALPVIQGINYKDMVVENATMAARLEGISGDTFTDICISNVTIGLAPKAKK 435

Query: 421 QPWTCTDVEGITSHVTPPPCNLLPDHGEEKITACKFPEDNLPIDTVELKKCSFQLSH 477
           QPWTCTD+EGITS VTPPPC+LLPD GEEKIT CKFPEDNLPID+V+LKKCS+Q SH
Sbjct: 436 QPWTCTDIEGITSQVTPPPCDLLPDQGEEKITTCKFPEDNLPIDSVQLKKCSYQFSH 492

[Close]

BLAST of Sgr016017.1 vs. ExPASy TrEMBL
Match: A0A5D3D2R4 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004840 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.2e-245
Identity = 409/477 (85.74%), Postives = 442/477 (92.66%), Query Frame = 0


Query: 1   MAMILL-VLVVTSSLTEVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVG 60
           M M++L V++  + + EVE R+ +  +  KA+IL+ +G EY AI+CRAH+ASIT+FGGVG
Sbjct: 16  MKMVILGVIMAAALMDEVEGRRGKVKKGKKAKILEGNGIEYGAINCRAHTASITEFGGVG 75

Query: 61  DGETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQ 120
           DG TSNTKAFQ A+  L QYA DGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQ
Sbjct: 76  DGVTSNTKAFQAAVERLGQYANDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQ 135

Query: 121 DPSEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQG 180
           DP+EWPVV+PLPSYGHGRDTSGGRYIS IFGTN+TDVVITGNNGTIDGQG RWWQLFHQG
Sbjct: 136 DPNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDGQGDRWWQLFHQG 195

Query: 181 KLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGI 240
           KLKYTRPYLIE+MYSSD+QIS+LTLLNSPAWNVHPVYSSN+L+QGITIIAPVRSPNTDGI
Sbjct: 196 KLKYTRPYLIELMYSSDIQISSLTLLNSPAWNVHPVYSSNILIQGITIIAPVRSPNTDGI 255

Query: 241 NPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALG 300
           NPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGI FG PTKQLV+RRL+CISPTSAVIALG
Sbjct: 256 NPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGLPTKQLVVRRLTCISPTSAVIALG 315

Query: 301 SEMSGGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYG 360
           SEMSGGIQDVRAEDIVA++SESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTG+YG
Sbjct: 316 SEMSGGIQDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGDYG 375

Query: 361 SHADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKK 420
           SHAD  YDPHALPVIQGINYRDMVVENATMAARLEGISGDTFT ICISNVTI +A KAKK
Sbjct: 376 SHADKNYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTDICISNVTIGLAPKAKK 435

Query: 421 QPWTCTDVEGITSHVTPPPCNLLPDHGEEKITACKFPEDNLPIDTVELKKCSFQLSH 477
           QPWTCTDVEGITS VTPPPC+LLPD GEEKIT CKFPEDNLPID V+LKKCS+Q SH
Sbjct: 436 QPWTCTDVEGITSQVTPPPCDLLPDQGEEKITTCKFPEDNLPIDAVQLKKCSYQFSH 492

[Close]

BLAST of Sgr016017.1 vs. TAIR 10
Match: AT3G61490.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 695.3 bits (1793), Expect = 3.5e-200
Identity = 330/482 (68.46%), Postives = 395/482 (81.95%), Query Frame = 0


Query: 1   MAMILLVLVVTSSLTEVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVGD 60
           +A I+ ++ +++ +T  + R+  +           + FEY AI CR+HSASIT++GGVGD
Sbjct: 5   LACIVTIITLSNLITISQGRRVSQ---------SFETFEYTAIICRSHSASITEYGGVGD 64

Query: 61  GETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQD 120
           G+T NTKAFQ A+ HLSQY+ +GGAQL+VPAG+WLTGSFNLTSHFTLFLH+DA+LLA+QD
Sbjct: 65  GKTLNTKAFQSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQD 124

Query: 121 PSEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQGK 180
            +E+P++K LPSYG GRD +GGR+ S IFGTN++DV+ITGNNGTIDGQG+ WWQ FH GK
Sbjct: 125 LNEYPILKALPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGK 184

Query: 181 LKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGIN 240
           LKYTRPYLIE+M+S  +QISNLT L+SP+WN+HPVYSSN++V+G+TIIAPV+SPNTDGIN
Sbjct: 185 LKYTRPYLIELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGIN 244

Query: 241 PDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALGS 300
           PDSCTN RIEDCYI+SGDDC+AVKSGWDEYGI+FG PTK LVIRRL+CISP SA IALGS
Sbjct: 245 PDSCTNTRIEDCYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGS 304

Query: 301 EMSGGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGS 360
           EMSGGI+DVRAEDI A  +ESGVRIKT +GRG +VK+IYV+ M +HTMKW FWMTGNY +
Sbjct: 305 EMSGGIEDVRAEDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKA 364

Query: 361 HADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKKQ 420
           HADS YDPHALP I GINYRD+V EN +MA RLEGISGD FTGICISN TISMAAK KK 
Sbjct: 365 HADSHYDPHALPEITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKA 424

Query: 421 PWTCTDVEGITSHVTPPPCNLLPDHGEEKIT-------ACKFPEDNLPIDTVELKKCSFQ 476
            W C+DVEG+TS V P PC+LL D  E + T        C FP D L ID VELK CS+Q
Sbjct: 425 IWMCSDVEGVTSGVDPKPCDLL-DGQESETTKKKMIDGGCDFPTDVLEIDNVELKTCSYQ 476

[Close]

BLAST of Sgr016017.1 vs. TAIR 10
Match: AT3G61490.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 695.3 bits (1793), Expect = 3.5e-200
Identity = 330/482 (68.46%), Postives = 395/482 (81.95%), Query Frame = 0


Query: 1   MAMILLVLVVTSSLTEVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVGD 60
           +A I+ ++ +++ +T  + R+  +           + FEY AI CR+HSASIT++GGVGD
Sbjct: 5   LACIVTIITLSNLITISQGRRVSQ---------SFETFEYTAIICRSHSASITEYGGVGD 64

Query: 61  GETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQD 120
           G+T NTKAFQ A+ HLSQY+ +GGAQL+VPAG+WLTGSFNLTSHFTLFLH+DA+LLA+QD
Sbjct: 65  GKTLNTKAFQSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQD 124

Query: 121 PSEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQGK 180
            +E+P++K LPSYG GRD +GGR+ S IFGTN++DV+ITGNNGTIDGQG+ WWQ FH GK
Sbjct: 125 LNEYPILKALPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGK 184

Query: 181 LKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGIN 240
           LKYTRPYLIE+M+S  +QISNLT L+SP+WN+HPVYSSN++V+G+TIIAPV+SPNTDGIN
Sbjct: 185 LKYTRPYLIELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGIN 244

Query: 241 PDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALGS 300
           PDSCTN RIEDCYI+SGDDC+AVKSGWDEYGI+FG PTK LVIRRL+CISP SA IALGS
Sbjct: 245 PDSCTNTRIEDCYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGS 304

Query: 301 EMSGGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGS 360
           EMSGGI+DVRAEDI A  +ESGVRIKT +GRG +VK+IYV+ M +HTMKW FWMTGNY +
Sbjct: 305 EMSGGIEDVRAEDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKA 364

Query: 361 HADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKKQ 420
           HADS YDPHALP I GINYRD+V EN +MA RLEGISGD FTGICISN TISMAAK KK 
Sbjct: 365 HADSHYDPHALPEITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKA 424

Query: 421 PWTCTDVEGITSHVTPPPCNLLPDHGEEKIT-------ACKFPEDNLPIDTVELKKCSFQ 476
            W C+DVEG+TS V P PC+LL D  E + T        C FP D L ID VELK CS+Q
Sbjct: 425 IWMCSDVEGVTSGVDPKPCDLL-DGQESETTKKKMIDGGCDFPTDVLEIDNVELKTCSYQ 476

[Close]

BLAST of Sgr016017.1 vs. TAIR 10
Match: AT3G61490.3 (Pectin lyase-like superfamily protein )

HSP 1 Score: 695.3 bits (1793), Expect = 3.5e-200
Identity = 330/482 (68.46%), Postives = 395/482 (81.95%), Query Frame = 0


Query: 1   MAMILLVLVVTSSLTEVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVGD 60
           +A I+ ++ +++ +T  + R+  +           + FEY AI CR+HSASIT++GGVGD
Sbjct: 5   LACIVTIITLSNLITISQGRRVSQ---------SFETFEYTAIICRSHSASITEYGGVGD 64

Query: 61  GETSNTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQD 120
           G+T NTKAFQ A+ HLSQY+ +GGAQL+VPAG+WLTGSFNLTSHFTLFLH+DA+LLA+QD
Sbjct: 65  GKTLNTKAFQSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQD 124

Query: 121 PSEWPVVKPLPSYGHGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQGK 180
            +E+P++K LPSYG GRD +GGR+ S IFGTN++DV+ITGNNGTIDGQG+ WWQ FH GK
Sbjct: 125 LNEYPILKALPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGK 184

Query: 181 LKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGIN 240
           LKYTRPYLIE+M+S  +QISNLT L+SP+WN+HPVYSSN++V+G+TIIAPV+SPNTDGIN
Sbjct: 185 LKYTRPYLIELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGIN 244

Query: 241 PDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALGS 300
           PDSCTN RIEDCYI+SGDDC+AVKSGWDEYGI+FG PTK LVIRRL+CISP SA IALGS
Sbjct: 245 PDSCTNTRIEDCYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGS 304

Query: 301 EMSGGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGS 360
           EMSGGI+DVRAEDI A  +ESGVRIKT +GRG +VK+IYV+ M +HTMKW FWMTGNY +
Sbjct: 305 EMSGGIEDVRAEDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKA 364

Query: 361 HADSKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKKQ 420
           HADS YDPHALP I GINYRD+V EN +MA RLEGISGD FTGICISN TISMAAK KK 
Sbjct: 365 HADSHYDPHALPEITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKA 424

Query: 421 PWTCTDVEGITSHVTPPPCNLLPDHGEEKIT-------ACKFPEDNLPIDTVELKKCSFQ 476
            W C+DVEG+TS V P PC+LL D  E + T        C FP D L ID VELK CS+Q
Sbjct: 425 IWMCSDVEGVTSGVDPKPCDLL-DGQESETTKKKMIDGGCDFPTDVLEIDNVELKTCSYQ 476

[Close]

BLAST of Sgr016017.1 vs. TAIR 10
Match: AT4G23500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 674.5 bits (1739), Expect = 6.4e-194
Identity = 316/456 (69.30%), Postives = 379/456 (83.11%), Query Frame = 0


Query: 15  TEVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVGDGETSNTKAFQEAIG 74
           T++ + + R+ RN        + FEY+AISCRA+SAS+ +FG VGDG TSNT AF++A+ 
Sbjct: 37  TDLHNHRHRRHRNHHR----GEEFEYSAISCRAYSASLDEFGAVGDGVTSNTAAFRDAVS 96

Query: 75  HLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDPSEWPVVKPLPSYG 134
            LS++A  GG+ L+VPAGRWLTG+FNLTSHFTLFLH+DAV+LASQ+ S++ V++PLPSYG
Sbjct: 97  QLSRFADYGGSLLFVPAGRWLTGNFNLTSHFTLFLHRDAVILASQEESDYEVIEPLPSYG 156

Query: 135 HGRDTSGGRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQGKLKYTRPYLIEIMYS 194
            GRDT GGR+IS +FG+N+TDVVITG NGTIDGQG  WW  F +G+LKYTRPYLIEIM+S
Sbjct: 157 RGRDTDGGRFISLLFGSNLTDVVITGENGTIDGQGEPWWGKFKRGELKYTRPYLIEIMHS 216

Query: 195 SDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGINPDSCTNVRIEDCYI 254
             +QISNLT LNSP+W++HPVYSSN+ +QG+TI+APV  PNTDGINPDSCTN RIEDCYI
Sbjct: 217 DGIQISNLTFLNSPSWHIHPVYSSNIYIQGLTILAPVTVPNTDGINPDSCTNTRIEDCYI 276

Query: 255 VSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALGSEMSGGIQDVRAEDI 314
           VSGDDC+AVKSGWD+YGI +G PTKQL+IRRL+CISP SAVIALGSEMSGGI+DVRAEDI
Sbjct: 277 VSGDDCIAVKSGWDQYGINYGMPTKQLLIRRLTCISPDSAVIALGSEMSGGIEDVRAEDI 336

Query: 315 VAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSHADSKYDPHALPVI 374
           VA++SESG+RIKT IGRGGYVKD+YVR MTM TMK+ FWMTG+YGSH D  YDP ALPVI
Sbjct: 337 VAINSESGIRIKTAIGRGGYVKDVYVRGMTMMTMKYVFWMTGSYGSHPDDHYDPKALPVI 396

Query: 375 QGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKKQPWTCTDVEGITSHV 434
           Q INY+DMV EN TM A+L GISGD FTGICISNVTI+++ K KK  W CTDV G TS V
Sbjct: 397 QNINYQDMVAENVTMPAQLAGISGDQFTGICISNVTITLSKKPKKVLWNCTDVSGYTSGV 456

Query: 435 TPPPCNLLPDHGEEKITACKFPEDNLPIDTVELKKC 471
           TP PC LLP+     +  C FPED +PID V+L++C
Sbjct: 457 TPQPCQLLPEKQPGTVVPCNFPEDPIPIDEVKLQRC 488

[Close]

BLAST of Sgr016017.1 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 626.7 bits (1615), Expect = 1.5e-179
Identity = 300/468 (64.10%), Postives = 363/468 (77.56%), Query Frame = 0


Query: 5   LLVLVVTSSLTEVESRKTRKVRNAKARILDADGFEYAAISCRAHSASITDFGGVGDGETS 64
           LLV +       +ESR  R    +K         E++A++CR HSA +TDFG VGDG+TS
Sbjct: 8   LLVFLAIFLFPAIESRSHRNSVTSK--------IEFSALNCRKHSAILTDFGAVGDGKTS 67

Query: 65  NTKAFQEAIGHLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLFLHQDAVLLASQDPSEW 124
           NTKAF+ AI  LSQ A DGGAQL VP G+WLTGSFNLTSHFTLF+ + A +LASQD SEW
Sbjct: 68  NTKAFRNAISKLSQMATDGGAQLVVPPGKWLTGSFNLTSHFTLFIQRGATILASQDESEW 127

Query: 125 PVVKPLPSYGHGRDTSG-GRYISFIFGTNVTDVVITGNNGTIDGQGARWWQLFHQGKLKY 184
           PV+ PLPSYG GRD +G GR+ S I GTN+TDVVITGNNGTI+GQG  WW  F + + K 
Sbjct: 128 PVIAPLPSYGKGRDGTGTGRFNSLISGTNLTDVVITGNNGTINGQGQYWWDKFKKKQFKI 187

Query: 185 TRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLVQGITIIAPVRSPNTDGINPDS 244
           TRPYLIEI++S ++QISN+TL++SP+WN+HPVY ++V+V+ +T++APV  PNTDGINPDS
Sbjct: 188 TRPYLIEILFSKNIQISNITLIDSPSWNIHPVYCNSVIVKSVTVLAPVTVPNTDGINPDS 247

Query: 245 CTNVRIEDCYIVSGDDCVAVKSGWDEYGIAFGWPTKQLVIRRLSCISPTSAVIALGSEMS 304
           CTN  IEDCYIVSGDDC+AVKSGWD+YGI FG PT+QL IRRL+CISP SA +ALGSEMS
Sbjct: 248 CTNTLIEDCYIVSGDDCIAVKSGWDQYGIKFGMPTQQLSIRRLTCISPKSAGVALGSEMS 307

Query: 305 GGIQDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGNYGSHAD 364
           GGI+DVR ED+   ++ES +RIKT +GRG YVKDIY RR+TM TMK+ FWM+GNYGSH D
Sbjct: 308 GGIKDVRIEDVTLTNTESAIRIKTAVGRGAYVKDIYARRITMKTMKYVFWMSGNYGSHPD 367

Query: 365 SKYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICISNVTISMAAKAKKQPWT 424
             +DP ALP I  INYRDM  EN TM+A L+GI  D FTGICISNVTI++AAKAKK  W 
Sbjct: 368 EGFDPKALPEITNINYRDMTAENVTMSASLDGIDKDPFTGICISNVTIALAAKAKKMQWN 427

Query: 425 CTDVEGITSHVTPPPCNLLPD-HGEEKITACKFPEDNLPIDTVELKKC 471
           CTDV G+TS VTP PC+LLP+   + K   C FP D +PI++V LKKC
Sbjct: 428 CTDVAGVTSRVTPEPCSLLPEKKAQAKNVDCAFPSDLIPIESVVLKKC 467

The following BLAST results are available for this feature:

BLAST of Sgr016017.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147419.1	1.2e-247	87.39	probable polygalacturonase [Momordica charantia]	[more]
XP_022974569.1	2.7e-247	87.79	probable polygalacturonase [Cucurbita maxima]	[more]
XP_022935413.1	3.0e-246	87.21	probable polygalacturonase [Cucurbita moschata]	[more]
XP_004133855.1	5.1e-246	85.74	probable polygalacturonase [Cucumis sativus] >KGN56496.1 hypothetical protein Cs...	[more]
XP_023526703.1	6.7e-246	87.13	probable polygalacturonase [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sgr016017.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7PZL3	5.0e-220	77.17	Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ...	[more]
Q7M1E7	1.7e-26	28.33	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
Q9LW07	1.2e-24	30.22	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
P27644	4.7e-24	33.33	Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1	[more]
Q8RY29	3.9e-23	26.93	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]

BLAST of Sgr016017.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D0Y3	5.9e-248	87.39	probable polygalacturonase OS=Momordica charantia OX=3673 GN=LOC111016350 PE=3 S...	[more]
A0A6J1II01	1.3e-247	87.79	probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111473265 PE=3 SV=1	[more]
A0A6J1FAH2	1.5e-246	87.21	probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111442298 PE=3 SV...	[more]
A0A0A0L8V4	2.5e-246	85.74	Pectate_lyase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G12...	[more]
A0A5D3D2R4	1.2e-245	85.74	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]

BLAST of Sgr016017.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G61490.1	3.5e-200	68.46	Pectin lyase-like superfamily protein	[more]
AT3G61490.2	3.5e-200	68.46	Pectin lyase-like superfamily protein	[more]
AT3G61490.3	3.5e-200	68.46	Pectin lyase-like superfamily protein	[more]
AT4G23500.1	6.4e-194	69.30	Pectin lyase-like superfamily protein	[more]
AT3G48950.1	1.5e-179	64.10	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 148..174 e-value: 5600.0 score: 1.9 coord: 335..356 e-value: 9300.0 score: 0.3 coord: 306..327 e-value: 8500.0 score: 0.6 coord: 217..243 e-value: 550.0 score: 9.1 coord: 244..265 e-value: 5.6 score: 15.9
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 145..428 e-value: 5.0E-38 score: 131.0
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 37..435 e-value: 1.2E-113 score: 382.2
None	No IPR available	PANTHER	PTHR31339:SF50	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 3..474
None	No IPR available	PANTHER	PTHR31339	PECTIN LYASE-RELATED	coord: 3..474
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 42..443

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sgr016017	Sgr016017	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sgr016017.1.exon1	Sgr016017.1.exon1	exon
Sgr016017.1.exon2	Sgr016017.1.exon2	exon
Sgr016017.1.exon3	Sgr016017.1.exon3	exon
Sgr016017.1.exon4	Sgr016017.1.exon4	exon
Sgr016017.1.exon5	Sgr016017.1.exon5	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Sgr016017.1	cds.Sgr016017.1	CDS
cds.Sgr016017.1	cds.Sgr016017.1_2	CDS
cds.Sgr016017.1	cds.Sgr016017.1_3	CDS
cds.Sgr016017.1	cds.Sgr016017.1_4	CDS
cds.Sgr016017.1	cds.Sgr016017.1_5	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sgr016017.1	Sgr016017.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity
molecular_function	GO:0008194	UDP-glycosyltransferase activity