Sed0025819.2 (mRNA) Chayote v1

Overview
NameSed0025819.2
TypemRNA
OrganismSechium edule (Chayote v1)
Descriptionmetal tolerance protein 1-like
LocationLG01: 66855255 .. 66859493 (-)
Sequence length1889
RNA-Seq ExpressionSed0025819.2
SyntenySed0025819.2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTCTAGAAAAGCTGAATATCTCAGATTGAAAGTGGCTGCGATTTACAAAGGAAAACCGGACGGCCGCCCATATTGCCGCAATCTCACTGCGCCTATAAAAACCACCAAAACCTCAATCGCCCGCCATTTTTCCCGATTAGGGCCTTTTGCAGCGTTCTTCTTCATTTCGCTTAGGTATTTCTCCATTCCTCTCTCAGATTCTTCTTCGATTTCACCTTTTGTTTGTTACACTTTTCCATTTACTTTAATCCTGGAATCAATCTGGTTTCGTTGACGCATGAGCTGAATATTCGATCGTAATCCCTTTTTCGATAAGTTCGCTTTTGGTTTCCGATTCGTTCTTATCTTTGACGTGATTTGCCTGCTTTATGGAGGGATTTTCGTTGTTAAACGTGGATTTGACTGCCTGCAATGTTGAATTTGATCGGCTATTGGTTTTAGGGTTCGTGGATTTCTTTAGTCTCTGTGTTTTCCTGTGAGTGGTTTTTTTTTTTTGAATCTATGATTGATTTGCTCTTTGTTTTTGTTGCTTAGCATTACTCTCTCTTTATTTGCCTCTGAAGCTATGAGCCTTGTGTGTTTCTTTTCTCGGTTTCAATGCTTGAGGACGAGAAATCATTTTTGGCGATTAAGAAATTTGTTCGAGGCTTTTGTTGTCTAGTCTTTCGTCTCAGGGCTTGGCTTCCCTCGGTTCACCTTGATTCAGGGGCTTATTGATTTTTAGAAGCTTTTTTGGCGGTTCCTTTGCCTTTGCTTTGTATTTGATCTTTTTCGTGGTTTTTTTTTGTTTGTTATAATTGGATTATTCATGGTTTGGTTTGGTTTTATTTGCTTTCTAGAGGTTGTATCTTTGAGCAAGAGCCTCTCTTCATATTATCAATGAAAAGTCTGGCTTCTTGTTTAAATTTTTTTTTTTTGGCGAGACTCGAGAGTGAAATAATAGCTGAATTTTTAGAGGAAAATACTCACCATTTTACAATTTTATAGAAGATATTGTCTTAATGGTGATTAATTGGTGGTGTGAAGTTTTTTCACTTTTTCATAATCTCTTGACCGGCTATCTTTATTATCTTCTGCAATTTTATACATTAATGAAGTTGTTTCTTACTCAAAAGTTGAAGAATGTGTTGTACAAACTTTATGAGTCCTTTGAGGATTCTTTTAAAAAAGGGGGAAACCTGGATGATTAACATTGAACTTCTATTGTTTGTTCGGCAGAAAAGTAGTTCAACTTCACCCAATAGTTTTATACTTGTATCATCTATAGTGGAGTGGAGTTGTTATTATGGATTTCAACCTTAAACATATCGACTCTCCAACTGTAGGTCTCTTTCATAGTGAATTGAATACCGTAAATTCTATTTTACTGTTGCAATGCTCTTTATGACAGCGAACTGAATACCCTTGCTCCAATATTGCTTTCTACCTAAATCTGAGCTACTGGTGATCATGCAATGCTTGTGTTTTACTTTTCGTTGTCTTTATGTGCATGCCTTTATCTACTTTTCATTCTTCTTCTCACATGTATTCTCCTCCTGTAGTTCTTGGCTGTTAATAAGTTCTTTTCTGCAGTTCATTAGTTTATATGTTCAGAGTGTTTGTTGAATTTGTTTGTTTTGATATCCGTGGATGTCCGGCTAGCTTACGTGCAACTCGACTATTCTCACGGGACAAACCGCCTGTTTTTTTAAGAAAACATATAGAAAATTAATATCTAAGTAGTTGACCGCCGTAGATGTAGTACATAATCCATTTTGATTTTGATAAATGCATTGTTTAACCACCATGAGTACTCTAGTGGTCAAAAGGATAATACGAAACCAAATAGGTTGATCTATGGTGGCCACCTACCTAAGAATTAATTTCCTACTGGTTTCCTTGACATTCAAATATTGTAGGGTTAGACAATGTATCCCGTGAAAATAGTCGAGGTGTGCGCAAGCTGGTCGGACACTCACCATTATTATAAAAAGGCCTTATTTGAGAGGTATTTATTTGTATATGTTCTGTATACAGATCGTCCGTTCTGGTTGATTGCAAATTTGAAATGAAAAACTTATTTTCATGATCTGAAGAATCAAGTTCATGTTTACTGCCTTATGCCTCCCTTCCTAACAGGCCTCAGCCGAGTATGTTCCTCTGCATAGCTAGCTAATTTGAAATGCTTTTGATCTGTATCATATTTGTTCACATTTAACATTTCTTACCCTTGAAATCTCGTTGCAATTATCTGTAGGGGATTTCAATTTTTTATTTCTATAATTACTCATGTGAGGAATTGTTGAGTCGTATTGTGTTAATCCTGGAAGAGATGGAGTGTCAGTTTATGTGTATGAAGGAAATGGCATCCTTTCAGCTAGCTTTCAAAGTTGAGAGCTTATATTAGTTCTATCGTCTTGTTATAATGAATCACAAGGCGTTATTAAGTTATAGATATCTTTCAATCTTAATTTTTCTTGTAGGATCAAGAATACTATTCATCTCACTAACAGATCTGAGATGTTTATGTTTCTGTTTTATATCACAAGCCTTGTAGCTGTAACTACGACTATTTTAGCTTGTTATCCTGATAATACTTCTGTTTTCTATATTTCTGTGTAAGACTTCTGTTAAACATTTTTTCTTGCCGAATCTCAGATGGAAGTGCAAAATCATGATCATGGTCACATAATTGAAGTATGTGGAGAGGTGCAAGCAGTAGGGCCTAGCATTGTGGGGAGTAAAATTTGTGGGGTGGCACCGTCTTGTGGATTTAAAGATGCAAAAAATAGTTCGAAAGATGCAGAGGAGCGATCTGCATCCATGCGGAAGCTTTTCATCGCAGTTGTTCTCTGCATCGTTTTTATGAGTGTAGAAGTAGTTGGAGGCATTAAAGCAAACAGTCTTGCAATATTGACCGATGCTGCTCATTTATTGTCAGACGTTGCTGCATTTGCCATTTCCTTGTTCTCACTCTGGGCCTCTGGCTGGGAGGCAACTCCGCGCCAGTCGTATGGCTTTTTCCGGATTGAAATACTTGGTGCTCTTGTTTCCATTCAGATGATTTGGCTTCTTGCTGGAATTCTTGTTTACGAAGCCATTGTTCGACTTATCAATGGTCCAGGAGAAGTTAAGGGGTTGCTCATGTTTGCTGTTTCTGCGTTCGGGTTAGTTGTTAACATAGCTATGGCTCTGCTGCTCGGTCACGAACATGGTCATGCCCATTCTCATGGTCACAGTCATGGACATGGTGATCATGACCATGGTCATGGCGGCCACGAACATGAACATGATCACCACCATGGGTTAAGTGTTACCACTCACCACCACCACCACCATGAAGAAGAAGAGGGAGCTGCCAGAAGTGTTGTCAAGCACCACCATGAAGAAAAGGAGGGAGCTGCCAGAAGTGGTGTTAAGCACCACCATGATCATGCTCACGAGGAATCCAGAACTACACCCTTACTTGAAGGTCGCTGCGACGAAGAAAATAATTCACAGAAGGTTACCAAGACAGAGAAAAAGCAACGAAACATAAACCTACAGGGGGCTTACCTTCACGTACTCGGAGATTCCATCCAAAGCATTGGGGTTATGATTGGTGGGGCAGTCATATGGTATAAACCCGAATACATGATTATCGATCTGATATGCACCCTCATTTTCTCTATAATTGTGCTCTACACAACAATTCAAATGCTCCGGAACATCCTCGAGGTTCTGATGGAGAGCACGCCTCGAGAGATCGATGCGACAAAGCTGGAGAAGGGCCTATGTGAGATGGACGAGGTAGTTGCAATCCATGAACTGCACATCTGGGCGATAACTGTCGGAAAGGTTCTGTTGGCATGCCATGTGATAATCAAGCCAGAGGCAAATGCGGACATGGTGTTGGACAAGGTTATAGAGTACATCAGAAGAGAGTACAACATCAGCCATGTCACTATTCAGATAGAGCGCCAGTAAATTTCAAGGTTGAATGAATGCTTGTAGGTATGGTTTAAAAGGAAGATTTTGCCTCTTTTCAATTCCCTTAGTTTTAATGTCTTCTCTTTTCTTTTTCTTTCTCTTTTTTAAGGTGGAAAAAAGAACTACTACTGAACGGAATTATTTTAGTATGGGGAATTGAAAGTTGGCTTTGTAAGAGTTTTTTTCATTTTTAATATCGGTGACTGTTCGGACCTGCGTATATGCACATAAGCCTGTTTAGAGTTTATTTGAAAGTGCTTCTAATAGAATAGTTCATTTCTTTTGGTTA

mRNA sequence

ATTTCTAGAAAAGCTGAATATCTCAGATTGAAAGTGGCTGCGATTTACAAAGGAAAACCGGACGGCCGCCCATATTGCCGCAATCTCACTGCGCCTATAAAAACCACCAAAACCTCAATCGCCCGCCATTTTTCCCGATTAGGGCCTTTTGCAGCGTTCTTCTTCATTTCGCTTAGATCGTCCGTTCTGGTTGATTGCAAATTTGAAATGAAAAACTTATTTTCATGATCTGAAGAATCAAGTTCATGTTTACTGCCTTATGCCTCCCTTCCTAACAGGCCTCAGCCGAATGGAAGTGCAAAATCATGATCATGGTCACATAATTGAAGTATGTGGAGAGGTGCAAGCAGTAGGGCCTAGCATTGTGGGGAGTAAAATTTGTGGGGTGGCACCGTCTTGTGGATTTAAAGATGCAAAAAATAGTTCGAAAGATGCAGAGGAGCGATCTGCATCCATGCGGAAGCTTTTCATCGCAGTTGTTCTCTGCATCGTTTTTATGAGTGTAGAAGTAGTTGGAGGCATTAAAGCAAACAGTCTTGCAATATTGACCGATGCTGCTCATTTATTGTCAGACGTTGCTGCATTTGCCATTTCCTTGTTCTCACTCTGGGCCTCTGGCTGGGAGGCAACTCCGCGCCAGTCGTATGGCTTTTTCCGGATTGAAATACTTGGTGCTCTTGTTTCCATTCAGATGATTTGGCTTCTTGCTGGAATTCTTGTTTACGAAGCCATTGTTCGACTTATCAATGGTCCAGGAGAAGTTAAGGGGTTGCTCATGTTTGCTGTTTCTGCGTTCGGGTTAGTTGTTAACATAGCTATGGCTCTGCTGCTCGGTCACGAACATGGTCATGCCCATTCTCATGGTCACAGTCATGGACATGGTGATCATGACCATGGTCATGGCGGCCACGAACATGAACATGATCACCACCATGGGTTAAGTGTTACCACTCACCACCACCACCACCATGAAGAAGAAGAGGGAGCTGCCAGAAGTGTTGTCAAGCACCACCATGAAGAAAAGGAGGGAGCTGCCAGAAGTGGTGTTAAGCACCACCATGATCATGCTCACGAGGAATCCAGAACTACACCCTTACTTGAAGGTCGCTGCGACGAAGAAAATAATTCACAGAAGGTTACCAAGACAGAGAAAAAGCAACGAAACATAAACCTACAGGGGGCTTACCTTCACGTACTCGGAGATTCCATCCAAAGCATTGGGGTTATGATTGGTGGGGCAGTCATATGGTATAAACCCGAATACATGATTATCGATCTGATATGCACCCTCATTTTCTCTATAATTGTGCTCTACACAACAATTCAAATGCTCCGGAACATCCTCGAGGTTCTGATGGAGAGCACGCCTCGAGAGATCGATGCGACAAAGCTGGAGAAGGGCCTATGTGAGATGGACGAGGTAGTTGCAATCCATGAACTGCACATCTGGGCGATAACTGTCGGAAAGGTTCTGTTGGCATGCCATGTGATAATCAAGCCAGAGGCAAATGCGGACATGGTGTTGGACAAGGTTATAGAGTACATCAGAAGAGAGTACAACATCAGCCATGTCACTATTCAGATAGAGCGCCAGTAAATTTCAAGGTTGAATGAATGCTTGTAGGTATGGTTTAAAAGGAAGATTTTGCCTCTTTTCAATTCCCTTAGTTTTAATGTCTTCTCTTTTCTTTTTCTTTCTCTTTTTTAAGGTGGAAAAAAGAACTACTACTGAACGGAATTATTTTAGTATGGGGAATTGAAAGTTGGCTTTGTAAGAGTTTTTTTCATTTTTAATATCGGTGACTGTTCGGACCTGCGTATATGCACATAAGCCTGTTTAGAGTTTATTTGAAAGTGCTTCTAATAGAATAGTTCATTTCTTTTGGTTA

Coding sequence (CDS)

ATGCCTCCCTTCCTAACAGGCCTCAGCCGAATGGAAGTGCAAAATCATGATCATGGTCACATAATTGAAGTATGTGGAGAGGTGCAAGCAGTAGGGCCTAGCATTGTGGGGAGTAAAATTTGTGGGGTGGCACCGTCTTGTGGATTTAAAGATGCAAAAAATAGTTCGAAAGATGCAGAGGAGCGATCTGCATCCATGCGGAAGCTTTTCATCGCAGTTGTTCTCTGCATCGTTTTTATGAGTGTAGAAGTAGTTGGAGGCATTAAAGCAAACAGTCTTGCAATATTGACCGATGCTGCTCATTTATTGTCAGACGTTGCTGCATTTGCCATTTCCTTGTTCTCACTCTGGGCCTCTGGCTGGGAGGCAACTCCGCGCCAGTCGTATGGCTTTTTCCGGATTGAAATACTTGGTGCTCTTGTTTCCATTCAGATGATTTGGCTTCTTGCTGGAATTCTTGTTTACGAAGCCATTGTTCGACTTATCAATGGTCCAGGAGAAGTTAAGGGGTTGCTCATGTTTGCTGTTTCTGCGTTCGGGTTAGTTGTTAACATAGCTATGGCTCTGCTGCTCGGTCACGAACATGGTCATGCCCATTCTCATGGTCACAGTCATGGACATGGTGATCATGACCATGGTCATGGCGGCCACGAACATGAACATGATCACCACCATGGGTTAAGTGTTACCACTCACCACCACCACCACCATGAAGAAGAAGAGGGAGCTGCCAGAAGTGTTGTCAAGCACCACCATGAAGAAAAGGAGGGAGCTGCCAGAAGTGGTGTTAAGCACCACCATGATCATGCTCACGAGGAATCCAGAACTACACCCTTACTTGAAGGTCGCTGCGACGAAGAAAATAATTCACAGAAGGTTACCAAGACAGAGAAAAAGCAACGAAACATAAACCTACAGGGGGCTTACCTTCACGTACTCGGAGATTCCATCCAAAGCATTGGGGTTATGATTGGTGGGGCAGTCATATGGTATAAACCCGAATACATGATTATCGATCTGATATGCACCCTCATTTTCTCTATAATTGTGCTCTACACAACAATTCAAATGCTCCGGAACATCCTCGAGGTTCTGATGGAGAGCACGCCTCGAGAGATCGATGCGACAAAGCTGGAGAAGGGCCTATGTGAGATGGACGAGGTAGTTGCAATCCATGAACTGCACATCTGGGCGATAACTGTCGGAAAGGTTCTGTTGGCATGCCATGTGATAATCAAGCCAGAGGCAAATGCGGACATGGTGTTGGACAAGGTTATAGAGTACATCAGAAGAGAGTACAACATCAGCCATGTCACTATTCAGATAGAGCGCCAGTAA

Protein sequence

MPPFLTGLSRMEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKLFIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALLLGHEHGHAHSHGHSHGHGDHDHGHGGHEHEHDHHHGLSVTTHHHHHHEEEEGAARSVVKHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQGAYLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQIERQ
Homology
BLAST of Sed0025819.2 vs. NCBI nr
Match: KAG6605616.1 (Metal tolerance protein A2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035528.1 Metal tolerance protein A2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 702.2 bits (1811), Expect = 2.9e-198
Identity = 381/439 (86.79%), Postives = 399/439 (90.89%), Query Frame = 0

Query: 11  MEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKLF 70
           MEVQNHDHGH+IEV G+VQAVGPSIVGSKICG APSCGFKDAKNSS D  ER+ASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 71  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 130
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 131 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 190
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 191 LGHEHGHAHSHGHSHGHGDHDHGHGGHEH---EHDHHHGLSVTTHHHHH-HEEEEGAARS 250
           LGHEHGHAHSHGHSHGHGDHDHGHG HEH   +H HHHG+SVT HHHHH H EEEGAA  
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240

Query: 251 VVKHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQ 310
            VKHHH            HHH HAHEES TTPLL      E++SQKVTKT+K++RNIN+Q
Sbjct: 241 GVKHHH------------HHHHHAHEESVTTPLL------ESHSQKVTKTQKRERNINVQ 300

Query: 311 GAYLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLM 370
           GAYLHVLGDSIQSIGVMIGGA+IWYKPEY I+DLICTLIFS+IVLYTTIQMLRNILEVLM
Sbjct: 301 GAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLM 360

Query: 371 ESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVI 430
           ESTPREIDATKLEKGLCEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VI
Sbjct: 361 ESTPREIDATKLEKGLCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVI 420

Query: 431 EYIRREYNISHVTIQIERQ 446
           EYI+REYNISHVTIQIERQ
Sbjct: 421 EYIKREYNISHVTIQIERQ 421

BLAST of Sed0025819.2 vs. NCBI nr
Match: XP_023534426.1 (metal tolerance protein 1-like [Cucurbita pepo subsp. pepo] >XP_023534427.1 metal tolerance protein 1-like [Cucurbita pepo subsp. pepo] >XP_023534428.1 metal tolerance protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 696.0 bits (1795), Expect = 2.0e-196
Identity = 379/438 (86.53%), Postives = 397/438 (90.64%), Query Frame = 0

Query: 11  MEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKLF 70
           MEVQNHDHGH+IEV G+VQAVGPSIVGSKICG APSCGFKDAKNSS D  ER+ASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 71  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 130
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 131 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 190
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 191 LGHEHGHAHSHGHSHGHGDHDHGHGGHEH---EHDHHHGLSVTTHHHHHHEEEEGAARSV 250
           LGHEHGHAHSHGHSHGHGDHDHGHG HEH   +H HHHG+SVT HHHH H          
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHPHP--------- 240

Query: 251 VKHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQG 310
              HHEE EGAA   VKHHH HAHEES TTPLL      E++SQKVTKT+K++RNIN+QG
Sbjct: 241 ---HHEE-EGAAGDSVKHHHHHAHEESVTTPLL------ESHSQKVTKTQKRERNINVQG 300

Query: 311 AYLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLME 370
           AYLHVLGDSIQSIGVMIGGA+IWYKPEY I+DLICTLIFS+IVLYTTIQMLRNILEVLME
Sbjct: 301 AYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLME 360

Query: 371 STPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIE 430
           STPREIDATKLEKGLCEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VIE
Sbjct: 361 STPREIDATKLEKGLCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIE 419

Query: 431 YIRREYNISHVTIQIERQ 446
           YI+REYNISHVTIQIERQ
Sbjct: 421 YIKREYNISHVTIQIERQ 419

BLAST of Sed0025819.2 vs. NCBI nr
Match: XP_022958195.1 (metal tolerance protein 1-like [Cucurbita moschata] >XP_022958198.1 metal tolerance protein 1-like [Cucurbita moschata] >XP_022958199.1 metal tolerance protein 1-like [Cucurbita moschata] >XP_022958200.1 metal tolerance protein 1-like [Cucurbita moschata] >XP_022958201.1 metal tolerance protein 1-like [Cucurbita moschata])

HSP 1 Score: 695.3 bits (1793), Expect = 3.5e-196
Identity = 377/439 (85.88%), Postives = 396/439 (90.21%), Query Frame = 0

Query: 11  MEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKLF 70
           MEVQN DHGH+IEVCG+VQAVGPSIVGSKICG APSCGFKDAKNSS D  ER+ASMRKLF
Sbjct: 1   MEVQNPDHGHMIEVCGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 71  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 130
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 131 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 190
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 191 LGHEHGHAHSHGHSHGHGDHDHGHGGHEH---EHDHHHGLSVTTHHHHH-HEEEEGAARS 250
           LGHEHGHAHSHGHSHGHGDHDHGHG HEH   +H HHHG+SVT HHHHH H EEEGAA  
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240

Query: 251 VVKHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQ 310
            VKH              HHH HAHEES TTPLL      E++SQKVT T+K++RNIN+Q
Sbjct: 241 GVKH--------------HHHHHAHEESVTTPLL------ESHSQKVTNTQKRERNINVQ 300

Query: 311 GAYLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLM 370
           GAYLHVLGDSIQSIGVMIGGA+IWYKPEY I+DLICTLIFS+IVLYTTIQMLRNILEVLM
Sbjct: 301 GAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLM 360

Query: 371 ESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVI 430
           ESTPREIDATKLEKGLCEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VI
Sbjct: 361 ESTPREIDATKLEKGLCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVI 419

Query: 431 EYIRREYNISHVTIQIERQ 446
           EYI+REYNISH+TIQIERQ
Sbjct: 421 EYIKREYNISHITIQIERQ 419

BLAST of Sed0025819.2 vs. NCBI nr
Match: XP_022995642.1 (metal tolerance protein 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 690.3 bits (1780), Expect = 1.1e-194
Identity = 375/439 (85.42%), Postives = 397/439 (90.43%), Query Frame = 0

Query: 10  RMEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKL 69
           +MEVQNHDHGH+IEV G+VQAVGPSIVGSKICG APSCGFKDAKNSS D  ER+ASMRKL
Sbjct: 2   KMEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKL 61

Query: 70  FIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY 129
           FIA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY
Sbjct: 62  FIAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY 121

Query: 130 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL 189
           GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL
Sbjct: 122 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL 181

Query: 190 LLGHEHGHAHSHGHSHGHGDHDHGHGGHEH---EHDHHHGLSVTTHHHHHHEEEEGAARS 249
           LLGHEHGHAHSHGHSHGHGDH+HG GGHEH   +H HHHG+SVT HHHHH          
Sbjct: 182 LLGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHHH---------- 241

Query: 250 VVKHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQ 309
               HHEE EGAA   VKH H HAHEES TTPLL      E++SQKVTKT+K++RNIN+Q
Sbjct: 242 ---PHHEE-EGAAGDSVKHQHHHAHEESVTTPLL------ESHSQKVTKTQKRERNINVQ 301

Query: 310 GAYLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLM 369
           GAYLHVLGDSIQS+GVM+GGA+IWYKPEY I+DLICTLIFS+IVLYTTIQMLRNILEVLM
Sbjct: 302 GAYLHVLGDSIQSVGVMVGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLM 361

Query: 370 ESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVI 429
           ESTPREIDATKLEKGLCEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VI
Sbjct: 362 ESTPREIDATKLEKGLCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVI 420

Query: 430 EYIRREYNISHVTIQIERQ 446
           EYI+REYNISHVTIQIERQ
Sbjct: 422 EYIKREYNISHVTIQIERQ 420

BLAST of Sed0025819.2 vs. NCBI nr
Match: XP_022995643.1 (metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995644.1 metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995646.1 metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995647.1 metal tolerance protein 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 689.5 bits (1778), Expect = 1.9e-194
Identity = 375/438 (85.62%), Postives = 396/438 (90.41%), Query Frame = 0

Query: 11  MEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKLF 70
           MEVQNHDHGH+IEV G+VQAVGPSIVGSKICG APSCGFKDAKNSS D  ER+ASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 71  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 130
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 131 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 190
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 191 LGHEHGHAHSHGHSHGHGDHDHGHGGHEH---EHDHHHGLSVTTHHHHHHEEEEGAARSV 250
           LGHEHGHAHSHGHSHGHGDH+HG GGHEH   +H HHHG+SVT HHHHH           
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHHH----------- 240

Query: 251 VKHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQG 310
              HHEE EGAA   VKH H HAHEES TTPLL      E++SQKVTKT+K++RNIN+QG
Sbjct: 241 --PHHEE-EGAAGDSVKHQHHHAHEESVTTPLL------ESHSQKVTKTQKRERNINVQG 300

Query: 311 AYLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLME 370
           AYLHVLGDSIQS+GVM+GGA+IWYKPEY I+DLICTLIFS+IVLYTTIQMLRNILEVLME
Sbjct: 301 AYLHVLGDSIQSVGVMVGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLME 360

Query: 371 STPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIE 430
           STPREIDATKLEKGLCEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VIE
Sbjct: 361 STPREIDATKLEKGLCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIE 418

Query: 431 YIRREYNISHVTIQIERQ 446
           YI+REYNISHVTIQIERQ
Sbjct: 421 YIKREYNISHVTIQIERQ 418

BLAST of Sed0025819.2 vs. ExPASy Swiss-Prot
Match: Q9LXS1 (Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2)

HSP 1 Score: 521.2 bits (1341), Expect = 1.2e-146
Identity = 288/437 (65.90%), Postives = 336/437 (76.89%), Query Frame = 0

Query: 10  RMEVQNHDHGHIIEVCGEVQAVGPSIVG-SKICGVAPSCGFKDAKNSSKDAEERSASMRK 69
           +M+   H+H H++++CGEV +   S+VG  K CG AP CGF DAK SS +A+ER+ASMRK
Sbjct: 15  KMKDHIHEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMRK 74

Query: 70  LFIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQS 129
           L IAV+LC +F+ VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGW+A P+QS
Sbjct: 75  LLIAVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQS 134

Query: 130 YGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMA 189
           YGFFRIEILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNIAMA
Sbjct: 135 YGFFRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMA 194

Query: 190 LLLGHEHGHAHSHGHSHGHGDHDHGHGGHEHEHDHHHGLSVTTHHHHHHEEEEGAARSVV 249
           +LLGH+HGH H H H +GHG          H HDH HG++ T                  
Sbjct: 195 ILLGHDHGHGHGHSHDNGHG----------HSHDHGHGIAAT------------------ 254

Query: 250 KHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQGA 309
                          +HHHD  H+ES+ + +L                +KKQRN+N+QGA
Sbjct: 255 ---------------EHHHDSGHDESQLSDVL--------------IEQKKQRNVNIQGA 314

Query: 310 YLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLMES 369
           YLHVLGDSIQS+GVMIGGA+IWYKPE+ I+DLICTL+FS+IVL TTI MLRNILEVLMES
Sbjct: 315 YLHVLGDSIQSVGVMIGGAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMES 374

Query: 370 TPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEY 429
           TPREID T LEKG+CE++EVVA+HELHIWAITVGK+LLACHV I+PEA ADMVLDK+I+Y
Sbjct: 375 TPREIDPTMLEKGVCEIEEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDY 393

Query: 430 IRREYNISHVTIQIERQ 446
           I+RE+NISHVTIQIERQ
Sbjct: 435 IKREHNISHVTIQIERQ 393

BLAST of Sed0025819.2 vs. ExPASy Swiss-Prot
Match: Q9ZT63 (Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2)

HSP 1 Score: 520.8 bits (1340), Expect = 1.5e-146
Identity = 300/434 (69.12%), Postives = 337/434 (77.65%), Query Frame = 0

Query: 11  MEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKLF 70
           ME  +  H HI+EV          IV SK+CG AP CGF D+KN+S DA ERSASMRKL 
Sbjct: 1   MESSSPHHSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLC 60

Query: 71  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 130
           IAVVLC+VFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATPRQ+YG
Sbjct: 61  IAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYG 120

Query: 131 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 190
           FFRIEILGALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVL 180

Query: 191 LGHEHGHAHSHGHSHGHGDHDHGHGGHEHEHDHHHGLSVTTHHHHHHEEEEGAARSVVKH 250
           LGH+HGH+H HGH HG   HDH        H+H HG++VTTHHHHH  E          H
Sbjct: 181 LGHDHGHSHGHGHGHG---HDH--------HNHSHGVTVTTHHHHHDHE----------H 240

Query: 251 HHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQGAYL 310
            H    G  +     HH H      T  LL      + +  +V   EK++RNINLQGAYL
Sbjct: 241 GHSHGHGEDK-----HHAHG---DVTEQLL------DKSKTQVAAKEKRKRNINLQGAYL 300

Query: 311 HVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLMESTP 370
           HVLGDSIQS+GVMIGGA+IWY PE+ I+DLICTL FS+IVL TTI M+RNILEVLMESTP
Sbjct: 301 HVLGDSIQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTP 360

Query: 371 REIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIR 430
           REIDATKLEKGL EM+EVVA+HELHIWAITVGKVLLACHV I+PEA+ADMVL+KVI+YIR
Sbjct: 361 REIDATKLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIR 398

Query: 431 REYNISHVTIQIER 445
           REYNISHVTIQIER
Sbjct: 421 REYNISHVTIQIER 398

BLAST of Sed0025819.2 vs. ExPASy Swiss-Prot
Match: Q688R1 (Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.7e-143
Identity = 301/441 (68.25%), Postives = 339/441 (76.87%), Query Frame = 0

Query: 11  MEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKLF 70
           M+  N     I EV  ++ +      G+K+C  A +C F D+ NSSKDA ER ASMRKL 
Sbjct: 1   MDSHNSAPPQIAEVRMDISSSTSVAAGNKVCRGA-ACDFSDSSNSSKDARERMASMRKLI 60

Query: 71  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 130
           IAV+LCI+FM+VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATP+QSYG
Sbjct: 61  IAVILCIIFMAVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPQQSYG 120

Query: 131 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 190
           FFRIEILGALVSIQ+IWLLAGILVYEAIVRLIN  GEV+G LMFAVSAFGL VNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLAGILVYEAIVRLINESGEVQGSLMFAVSAFGLFVNIIMAVL 180

Query: 191 LGHEHGHAHSHGHSHGHGDHDHGHGGHEHEHDHH----HGLSVTTHHHHHHEEEEGAARS 250
           LGH+HGH H HGH HGH  HDH HGG +H+H HH    HG      H HHHE+  G + +
Sbjct: 181 LGHDHGHGHGHGHGHGH-SHDHDHGGSDHDHHHHEDQEHG------HVHHHEDGHGNSIT 240

Query: 251 VVKHHHEEKEGAARSGVKHHHDHAHEESRTTPLL--EGRCDEENNSQKVTKTEKKQRNIN 310
           V  HHH         G  HHH  A E     PLL  +  CD   +  K  K  K +RNIN
Sbjct: 241 VNLHHH--------PGTGHHHHDAEE-----PLLKSDAGCDSTQSGAKDAK--KARRNIN 300

Query: 311 LQGAYLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEV 370
           +  AYLHVLGDSIQSIGVMIGGA+IWYKPE+ IIDLICTLIFS+IVL+TTI+MLRNILEV
Sbjct: 301 VHSAYLHVLGDSIQSIGVMIGGAIIWYKPEWKIIDLICTLIFSVIVLFTTIKMLRNILEV 360

Query: 371 LMESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDK 430
           LMESTPREIDAT LE GL +MD VVA+HELHIWAITVGKVLLACHV I  +A+AD +LDK
Sbjct: 361 LMESTPREIDATSLENGLRDMDGVVAVHELHIWAITVGKVLLACHVTITQDADADQMLDK 418

Query: 431 VIEYIRREYNISHVTIQIERQ 446
           VI YI+ EYNISHVTIQIER+
Sbjct: 421 VIGYIKSEYNISHVTIQIERE 418

BLAST of Sed0025819.2 vs. ExPASy Swiss-Prot
Match: Q9M271 (Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 5.7e-109
Identity = 231/408 (56.62%), Postives = 274/408 (67.16%), Query Frame = 0

Query: 38  SKICGVAPSCGFKDAKNSSKDAEERSASMRKLFIAVVLCIVFMSVEVVGGIKANSLAILT 97
           SK+CG   +CGF     SS DA++R+ASMRKL   VVLC++FMS+EVV GIKANSLAIL 
Sbjct: 6   SKVCG-ETACGF---STSSSDAKKRAASMRKLCFVVVLCLLFMSIEVVCGIKANSLAILA 65

Query: 98  DAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEA 157
           DAAHLL+DV AFAIS+ SLWAS WEA PRQSYGFFRIEILG LVSIQ+IWLL GILVYEA
Sbjct: 66  DAAHLLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEA 125

Query: 158 IVRLINGPG-EVKGLLMFAVSAFGLVVNIAMALLLGHEHGHAHSHGHSHGHGDHDHGHGG 217
           + RL+     +V G  M  V+AFGLVVNI M ++LGH+HGH H HGHSH   DH H +G 
Sbjct: 126 VTRLVQETNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHGHSH---DHGHSYG- 185

Query: 218 HEHEHDHHHGLSVTTHHHHHHEEEEGAARSVVKHHHEEKEGAARSGVKHHHDHAHEESRT 277
                                                                       
Sbjct: 186 ------------------------------------------------------------ 245

Query: 278 TPLLEGRCDEENNSQKVTKTEKKQRNINLQGAYLHVLGDSIQSIGVMIGGAVIWYKPEYM 337
                        ++++ +  K+ RNIN+QGAYLHVLGD IQSIGVMIGG +IWY P++ 
Sbjct: 246 -----------ERAEQLLEKSKEIRNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKWK 305

Query: 338 IIDLICTLIFSIIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVVAIHELHI 397
           +IDLICTL FS+IVL TTI+MLR+ILEVLMESTPREIDA +LEKGL E++EVV +HELHI
Sbjct: 306 VIDLICTLFFSVIVLGTTIKMLRSILEVLMESTPREIDARQLEKGLMEIEEVVDVHELHI 334

Query: 398 WAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQIER 445
           WAITVGK L +CHV ++PEA  +MVL+KVI+YI REY ISHVTIQIER
Sbjct: 366 WAITVGKALFSCHVKVRPEAGDEMVLNKVIDYIWREYRISHVTIQIER 334

BLAST of Sed0025819.2 vs. ExPASy Swiss-Prot
Match: Q6DBM8 (Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.1e-80
Identity = 170/419 (40.57%), Postives = 251/419 (59.90%), Query Frame = 0

Query: 27  EVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKLFIAVVLCIVFMSVEVVG 86
           E+++  PS     +  V  SC F   ++   + +ER  S R+L   + L ++ MSV++VG
Sbjct: 16  EMESPSPSKTEENLGVVPLSCAFTRQEHCVSETKEREESTRRLSSLIFLYLIVMSVQIVG 75

Query: 87  GIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMI 146
           G KANSLA++TDAAHLLSDVA   +SL ++  S WEA PR S+GF R+E+L A +S+Q+I
Sbjct: 76  GFKANSLAVMTDAAHLLSDVAGLCVSLLAIKVSSWEANPRNSFGFKRLEVLAAFLSVQLI 135

Query: 147 WLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALLLGHEHGHAHSHGHSHG 206
           WL++G++++EAI RL++   EV G +MF +SAFG  +N+ M L LGH H H         
Sbjct: 136 WLVSGVIIHEAIQRLLSRSREVNGEIMFGISAFGFFMNLVMVLWLGHNHSH--------- 195

Query: 207 HGDHDHGHGGHEHEHDHHHGLSVTTHHHHHHEEEEGAARSVVKHHHEEKEGAARSGVKHH 266
                       H HDHH       HHHH+H                          KH 
Sbjct: 196 ------------HHHDHH-------HHHHNH--------------------------KHQ 255

Query: 267 HDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQGAYLHVLGDSIQSIGVMIGG 326
           H H H+E     ++    +EE N  K  K+  K+ NIN+QGAYLH + D IQS+GVMIGG
Sbjct: 256 HQHHHKE-----VVAEEEEEEMNPLKGEKSSSKEMNINIQGAYLHAMADMIQSLGVMIGG 315

Query: 327 AVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCEMD 386
            +IW KP+++++DLICTL+FS   L  T+ +L+NI  +LME  PR++D  KLE+GL  +D
Sbjct: 316 GIIWVKPKWVLVDLICTLVFSAFALAATLPILKNIFGILMERVPRDMDIEKLERGLKRID 375

Query: 387 EVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQIERQ 446
            V  +++LH+W ITVG+++L+CH++ +P A+   ++  V  + R+ Y I H T+Q+E +
Sbjct: 376 GVKIVYDLHVWEITVGRIVLSCHILPEPGASPKEIITGVRNFCRKSYGIYHATVQVESE 375

BLAST of Sed0025819.2 vs. ExPASy TrEMBL
Match: A0A6J1H2S0 (metal tolerance protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459497 PE=4 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.7e-196
Identity = 377/439 (85.88%), Postives = 396/439 (90.21%), Query Frame = 0

Query: 11  MEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKLF 70
           MEVQN DHGH+IEVCG+VQAVGPSIVGSKICG APSCGFKDAKNSS D  ER+ASMRKLF
Sbjct: 1   MEVQNPDHGHMIEVCGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 71  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 130
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 131 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 190
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 191 LGHEHGHAHSHGHSHGHGDHDHGHGGHEH---EHDHHHGLSVTTHHHHH-HEEEEGAARS 250
           LGHEHGHAHSHGHSHGHGDHDHGHG HEH   +H HHHG+SVT HHHHH H EEEGAA  
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240

Query: 251 VVKHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQ 310
            VKH              HHH HAHEES TTPLL      E++SQKVT T+K++RNIN+Q
Sbjct: 241 GVKH--------------HHHHHAHEESVTTPLL------ESHSQKVTNTQKRERNINVQ 300

Query: 311 GAYLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLM 370
           GAYLHVLGDSIQSIGVMIGGA+IWYKPEY I+DLICTLIFS+IVLYTTIQMLRNILEVLM
Sbjct: 301 GAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLM 360

Query: 371 ESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVI 430
           ESTPREIDATKLEKGLCEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VI
Sbjct: 361 ESTPREIDATKLEKGLCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVI 419

Query: 431 EYIRREYNISHVTIQIERQ 446
           EYI+REYNISH+TIQIERQ
Sbjct: 421 EYIKREYNISHITIQIERQ 419

BLAST of Sed0025819.2 vs. ExPASy TrEMBL
Match: A0A6J1JZI0 (metal tolerance protein 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491116 PE=4 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 5.4e-195
Identity = 375/439 (85.42%), Postives = 397/439 (90.43%), Query Frame = 0

Query: 10  RMEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKL 69
           +MEVQNHDHGH+IEV G+VQAVGPSIVGSKICG APSCGFKDAKNSS D  ER+ASMRKL
Sbjct: 2   KMEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKL 61

Query: 70  FIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY 129
           FIA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY
Sbjct: 62  FIAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY 121

Query: 130 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL 189
           GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL
Sbjct: 122 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL 181

Query: 190 LLGHEHGHAHSHGHSHGHGDHDHGHGGHEH---EHDHHHGLSVTTHHHHHHEEEEGAARS 249
           LLGHEHGHAHSHGHSHGHGDH+HG GGHEH   +H HHHG+SVT HHHHH          
Sbjct: 182 LLGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHHH---------- 241

Query: 250 VVKHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQ 309
               HHEE EGAA   VKH H HAHEES TTPLL      E++SQKVTKT+K++RNIN+Q
Sbjct: 242 ---PHHEE-EGAAGDSVKHQHHHAHEESVTTPLL------ESHSQKVTKTQKRERNINVQ 301

Query: 310 GAYLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLM 369
           GAYLHVLGDSIQS+GVM+GGA+IWYKPEY I+DLICTLIFS+IVLYTTIQMLRNILEVLM
Sbjct: 302 GAYLHVLGDSIQSVGVMVGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLM 361

Query: 370 ESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVI 429
           ESTPREIDATKLEKGLCEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VI
Sbjct: 362 ESTPREIDATKLEKGLCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVI 420

Query: 430 EYIRREYNISHVTIQIERQ 446
           EYI+REYNISHVTIQIERQ
Sbjct: 422 EYIKREYNISHVTIQIERQ 420

BLAST of Sed0025819.2 vs. ExPASy TrEMBL
Match: A0A6J1JZI6 (metal tolerance protein 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491116 PE=4 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 9.3e-195
Identity = 375/438 (85.62%), Postives = 396/438 (90.41%), Query Frame = 0

Query: 11  MEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKLF 70
           MEVQNHDHGH+IEV G+VQAVGPSIVGSKICG APSCGFKDAKNSS D  ER+ASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 71  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 130
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 131 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 190
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 191 LGHEHGHAHSHGHSHGHGDHDHGHGGHEH---EHDHHHGLSVTTHHHHHHEEEEGAARSV 250
           LGHEHGHAHSHGHSHGHGDH+HG GGHEH   +H HHHG+SVT HHHHH           
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHHH----------- 240

Query: 251 VKHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQG 310
              HHEE EGAA   VKH H HAHEES TTPLL      E++SQKVTKT+K++RNIN+QG
Sbjct: 241 --PHHEE-EGAAGDSVKHQHHHAHEESVTTPLL------ESHSQKVTKTQKRERNINVQG 300

Query: 311 AYLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLME 370
           AYLHVLGDSIQS+GVM+GGA+IWYKPEY I+DLICTLIFS+IVLYTTIQMLRNILEVLME
Sbjct: 301 AYLHVLGDSIQSVGVMVGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLME 360

Query: 371 STPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIE 430
           STPREIDATKLEKGLCEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VIE
Sbjct: 361 STPREIDATKLEKGLCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIE 418

Query: 431 YIRREYNISHVTIQIERQ 446
           YI+REYNISHVTIQIERQ
Sbjct: 421 YIKREYNISHVTIQIERQ 418

BLAST of Sed0025819.2 vs. ExPASy TrEMBL
Match: A0A0A0KD36 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G080940 PE=4 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 2.5e-192
Identity = 376/444 (84.68%), Postives = 395/444 (88.96%), Query Frame = 0

Query: 5   LTGLSRMEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSA 64
           LT   +MEVQ  DHGHIIEVCG+VQAVGPSIVGSKICG APSCGF DAKNSSKDA+ERSA
Sbjct: 2   LTTQLQMEVQ--DHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSKDAKERSA 61

Query: 65  SMRKLFIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEAT 124
           SMRKL IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEAT
Sbjct: 62  SMRKLCIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEAT 121

Query: 125 PRQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVN 184
           PRQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKG LMFAVS FGLVVN
Sbjct: 122 PRQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGFLMFAVSTFGLVVN 181

Query: 185 IAMALLLGHEHGHAHSHGHSHGHGDHDHGHGGHEH---EHDHHHGLSVTTHHHHHHEEEE 244
           IAMALLLGHEHGHAHSHGH+HGHG+HDHGHG HEH   +H H HG+SVT HHHHH  EE+
Sbjct: 182 IAMALLLGHEHGHAHSHGHNHGHGEHDHGHGSHEHGEEDHRHRHGISVTMHHHHH--EEK 241

Query: 245 GAARSVVKHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQR 304
            AA   V+HHH            HHH H H+ES T PLL+       +SQKVTK +KKQR
Sbjct: 242 RAASDGVEHHH------------HHHHHKHKESTTVPLLD-------SSQKVTKAQKKQR 301

Query: 305 NINLQGAYLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNI 364
           NIN+QGAYLHVLGDSIQSIGVMIGGA+IWYKPEYMI+DLICTLIFS IVL TTIQMLRNI
Sbjct: 302 NINVQGAYLHVLGDSIQSIGVMIGGAIIWYKPEYMILDLICTLIFSAIVLCTTIQMLRNI 361

Query: 365 LEVLMESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMV 424
           LEVLMESTPRE+DATKLEKGLCEM+EVVAIHELHIWAITVGK+LLACHVIIKPEANADMV
Sbjct: 362 LEVLMESTPREVDATKLEKGLCEMEEVVAIHELHIWAITVGKILLACHVIIKPEANADMV 421

Query: 425 LDKVIEYIRREYNISHVTIQIERQ 446
           LDKVIEYIRREYNISHVTIQIERQ
Sbjct: 422 LDKVIEYIRREYNISHVTIQIERQ 422

BLAST of Sed0025819.2 vs. ExPASy TrEMBL
Match: A7L8D2 (Metal tolerance protein OS=Cucumis sativus OX=3659 GN=MTP PE=2 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.3e-191
Identity = 374/438 (85.39%), Postives = 392/438 (89.50%), Query Frame = 0

Query: 11  MEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKLF 70
           MEVQ  DHGHIIEVCG+VQAVGPSIVGSKICG APSCGF DAKNSSKDA+ERSASMRKL 
Sbjct: 1   MEVQ--DHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSKDAKERSASMRKLC 60

Query: 71  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 130
           IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 131 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 190
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKG LMFAVS FGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGFLMFAVSTFGLVVNIAMALL 180

Query: 191 LGHEHGHAHSHGHSHGHGDHDHGHGGHEH---EHDHHHGLSVTTHHHHHHEEEEGAARSV 250
           LGHEHGHAHSHGH+HGHG+HDHGHG HEH   +H H HG+SVT HHHHH  EE+ AA   
Sbjct: 181 LGHEHGHAHSHGHNHGHGEHDHGHGSHEHGEEDHRHRHGISVTMHHHHH--EEKRAASDG 240

Query: 251 VKHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQG 310
           V+HHH            HHH H H+ES T PLL+       +SQKVTK +KKQRNIN+QG
Sbjct: 241 VEHHH------------HHHHHKHKESTTVPLLD-------SSQKVTKAQKKQRNINVQG 300

Query: 311 AYLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLME 370
           AYLHVLGDSIQSIGVMIGGA+IWYKPEYMI+DLICTLIFS IVL TTIQMLRNILEVLME
Sbjct: 301 AYLHVLGDSIQSIGVMIGGAIIWYKPEYMILDLICTLIFSAIVLCTTIQMLRNILEVLME 360

Query: 371 STPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIE 430
           STPRE+DATKLEKGLCEM+EVVAIHELHIWAITVGK+LLACHVIIKPEANADMVLDKVIE
Sbjct: 361 STPREVDATKLEKGLCEMEEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIE 415

Query: 431 YIRREYNISHVTIQIERQ 446
           YIRREYNISHVTIQIERQ
Sbjct: 421 YIRREYNISHVTIQIERQ 415

BLAST of Sed0025819.2 vs. TAIR 10
Match: AT3G58810.2 (metal tolerance protein A2 )

HSP 1 Score: 521.2 bits (1341), Expect = 8.4e-148
Identity = 288/437 (65.90%), Postives = 336/437 (76.89%), Query Frame = 0

Query: 10  RMEVQNHDHGHIIEVCGEVQAVGPSIVG-SKICGVAPSCGFKDAKNSSKDAEERSASMRK 69
           +M+   H+H H++++CGEV +   S+VG  K CG AP CGF DAK SS +A+ER+ASMRK
Sbjct: 15  KMKDHIHEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMRK 74

Query: 70  LFIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQS 129
           L IAV+LC +F+ VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGW+A P+QS
Sbjct: 75  LLIAVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQS 134

Query: 130 YGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMA 189
           YGFFRIEILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNIAMA
Sbjct: 135 YGFFRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMA 194

Query: 190 LLLGHEHGHAHSHGHSHGHGDHDHGHGGHEHEHDHHHGLSVTTHHHHHHEEEEGAARSVV 249
           +LLGH+HGH H H H +GHG          H HDH HG++ T                  
Sbjct: 195 ILLGHDHGHGHGHSHDNGHG----------HSHDHGHGIAAT------------------ 254

Query: 250 KHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQGA 309
                          +HHHD  H+ES+ + +L                +KKQRN+N+QGA
Sbjct: 255 ---------------EHHHDSGHDESQLSDVL--------------IEQKKQRNVNIQGA 314

Query: 310 YLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLMES 369
           YLHVLGDSIQS+GVMIGGA+IWYKPE+ I+DLICTL+FS+IVL TTI MLRNILEVLMES
Sbjct: 315 YLHVLGDSIQSVGVMIGGAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMES 374

Query: 370 TPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEY 429
           TPREID T LEKG+CE++EVVA+HELHIWAITVGK+LLACHV I+PEA ADMVLDK+I+Y
Sbjct: 375 TPREIDPTMLEKGVCEIEEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDY 393

Query: 430 IRREYNISHVTIQIERQ 446
           I+RE+NISHVTIQIERQ
Sbjct: 435 IKREHNISHVTIQIERQ 393

BLAST of Sed0025819.2 vs. TAIR 10
Match: AT2G46800.1 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 520.8 bits (1340), Expect = 1.1e-147
Identity = 300/434 (69.12%), Postives = 337/434 (77.65%), Query Frame = 0

Query: 11  MEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKLF 70
           ME  +  H HI+EV          IV SK+CG AP CGF D+KN+S DA ERSASMRKL 
Sbjct: 1   MESSSPHHSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLC 60

Query: 71  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 130
           IAVVLC+VFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATPRQ+YG
Sbjct: 61  IAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYG 120

Query: 131 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 190
           FFRIEILGALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVL 180

Query: 191 LGHEHGHAHSHGHSHGHGDHDHGHGGHEHEHDHHHGLSVTTHHHHHHEEEEGAARSVVKH 250
           LGH+HGH+H HGH HG   HDH        H+H HG++VTTHHHHH  E          H
Sbjct: 181 LGHDHGHSHGHGHGHG---HDH--------HNHSHGVTVTTHHHHHDHE----------H 240

Query: 251 HHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQGAYL 310
            H    G  +     HH H      T  LL      + +  +V   EK++RNINLQGAYL
Sbjct: 241 GHSHGHGEDK-----HHAHG---DVTEQLL------DKSKTQVAAKEKRKRNINLQGAYL 300

Query: 311 HVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLMESTP 370
           HVLGDSIQS+GVMIGGA+IWY PE+ I+DLICTL FS+IVL TTI M+RNILEVLMESTP
Sbjct: 301 HVLGDSIQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTP 360

Query: 371 REIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIR 430
           REIDATKLEKGL EM+EVVA+HELHIWAITVGKVLLACHV I+PEA+ADMVL+KVI+YIR
Sbjct: 361 REIDATKLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIR 398

Query: 431 REYNISHVTIQIER 445
           REYNISHVTIQIER
Sbjct: 421 REYNISHVTIQIER 398

BLAST of Sed0025819.2 vs. TAIR 10
Match: AT2G46800.2 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 520.8 bits (1340), Expect = 1.1e-147
Identity = 300/434 (69.12%), Postives = 337/434 (77.65%), Query Frame = 0

Query: 11  MEVQNHDHGHIIEVCGEVQAVGPSIVGSKICGVAPSCGFKDAKNSSKDAEERSASMRKLF 70
           ME  +  H HI+EV          IV SK+CG AP CGF D+KN+S DA ERSASMRKL 
Sbjct: 1   MESSSPHHSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLC 60

Query: 71  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 130
           IAVVLC+VFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATPRQ+YG
Sbjct: 61  IAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYG 120

Query: 131 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 190
           FFRIEILGALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVL 180

Query: 191 LGHEHGHAHSHGHSHGHGDHDHGHGGHEHEHDHHHGLSVTTHHHHHHEEEEGAARSVVKH 250
           LGH+HGH+H HGH HG   HDH        H+H HG++VTTHHHHH  E          H
Sbjct: 181 LGHDHGHSHGHGHGHG---HDH--------HNHSHGVTVTTHHHHHDHE----------H 240

Query: 251 HHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQGAYL 310
            H    G  +     HH H      T  LL      + +  +V   EK++RNINLQGAYL
Sbjct: 241 GHSHGHGEDK-----HHAHG---DVTEQLL------DKSKTQVAAKEKRKRNINLQGAYL 300

Query: 311 HVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLMESTP 370
           HVLGDSIQS+GVMIGGA+IWY PE+ I+DLICTL FS+IVL TTI M+RNILEVLMESTP
Sbjct: 301 HVLGDSIQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTP 360

Query: 371 REIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIR 430
           REIDATKLEKGL EM+EVVA+HELHIWAITVGKVLLACHV I+PEA+ADMVL+KVI+YIR
Sbjct: 361 REIDATKLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIR 398

Query: 431 REYNISHVTIQIER 445
           REYNISHVTIQIER
Sbjct: 421 REYNISHVTIQIER 398

BLAST of Sed0025819.2 vs. TAIR 10
Match: AT3G58810.1 (metal tolerance protein A2 )

HSP 1 Score: 520.8 bits (1340), Expect = 1.1e-147
Identity = 288/437 (65.90%), Postives = 336/437 (76.89%), Query Frame = 0

Query: 10  RMEVQNHDHGHIIEVCGEVQAVGPSIVG-SKICGVAPSCGFKDAKNSSKDAEERSASMRK 69
           +M+   H+H H++++CGEV +   S+VG  K CG AP CGF DAK SS +A+ER+ASMRK
Sbjct: 54  QMKDHIHEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMRK 113

Query: 70  LFIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQS 129
           L IAV+LC +F+ VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGW+A P+QS
Sbjct: 114 LLIAVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQS 173

Query: 130 YGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMA 189
           YGFFRIEILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNIAMA
Sbjct: 174 YGFFRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMA 233

Query: 190 LLLGHEHGHAHSHGHSHGHGDHDHGHGGHEHEHDHHHGLSVTTHHHHHHEEEEGAARSVV 249
           +LLGH+HGH H H H +GHG          H HDH HG++ T                  
Sbjct: 234 ILLGHDHGHGHGHSHDNGHG----------HSHDHGHGIAAT------------------ 293

Query: 250 KHHHEEKEGAARSGVKHHHDHAHEESRTTPLLEGRCDEENNSQKVTKTEKKQRNINLQGA 309
                          +HHHD  H+ES+ + +L                +KKQRN+N+QGA
Sbjct: 294 ---------------EHHHDSGHDESQLSDVL--------------IEQKKQRNVNIQGA 353

Query: 310 YLHVLGDSIQSIGVMIGGAVIWYKPEYMIIDLICTLIFSIIVLYTTIQMLRNILEVLMES 369
           YLHVLGDSIQS+GVMIGGA+IWYKPE+ I+DLICTL+FS+IVL TTI MLRNILEVLMES
Sbjct: 354 YLHVLGDSIQSVGVMIGGAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMES 413

Query: 370 TPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEY 429
           TPREID T LEKG+CE++EVVA+HELHIWAITVGK+LLACHV I+PEA ADMVLDK+I+Y
Sbjct: 414 TPREIDPTMLEKGVCEIEEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDY 432

Query: 430 IRREYNISHVTIQIERQ 446
           I+RE+NISHVTIQIERQ
Sbjct: 474 IKREHNISHVTIQIERQ 432

BLAST of Sed0025819.2 vs. TAIR 10
Match: AT3G61940.1 (Cation efflux family protein )

HSP 1 Score: 396.0 bits (1016), Expect = 4.1e-110
Identity = 231/408 (56.62%), Postives = 274/408 (67.16%), Query Frame = 0

Query: 38  SKICGVAPSCGFKDAKNSSKDAEERSASMRKLFIAVVLCIVFMSVEVVGGIKANSLAILT 97
           SK+CG   +CGF     SS DA++R+ASMRKL   VVLC++FMS+EVV GIKANSLAIL 
Sbjct: 6   SKVCG-ETACGF---STSSSDAKKRAASMRKLCFVVVLCLLFMSIEVVCGIKANSLAILA 65

Query: 98  DAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEA 157
           DAAHLL+DV AFAIS+ SLWAS WEA PRQSYGFFRIEILG LVSIQ+IWLL GILVYEA
Sbjct: 66  DAAHLLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEA 125

Query: 158 IVRLINGPG-EVKGLLMFAVSAFGLVVNIAMALLLGHEHGHAHSHGHSHGHGDHDHGHGG 217
           + RL+     +V G  M  V+AFGLVVNI M ++LGH+HGH H HGHSH   DH H +G 
Sbjct: 126 VTRLVQETNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHGHSH---DHGHSYG- 185

Query: 218 HEHEHDHHHGLSVTTHHHHHHEEEEGAARSVVKHHHEEKEGAARSGVKHHHDHAHEESRT 277
                                                                       
Sbjct: 186 ------------------------------------------------------------ 245

Query: 278 TPLLEGRCDEENNSQKVTKTEKKQRNINLQGAYLHVLGDSIQSIGVMIGGAVIWYKPEYM 337
                        ++++ +  K+ RNIN+QGAYLHVLGD IQSIGVMIGG +IWY P++ 
Sbjct: 246 -----------ERAEQLLEKSKEIRNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKWK 305

Query: 338 IIDLICTLIFSIIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVVAIHELHI 397
           +IDLICTL FS+IVL TTI+MLR+ILEVLMESTPREIDA +LEKGL E++EVV +HELHI
Sbjct: 306 VIDLICTLFFSVIVLGTTIKMLRSILEVLMESTPREIDARQLEKGLMEIEEVVDVHELHI 334

Query: 398 WAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQIER 445
           WAITVGK L +CHV ++PEA  +MVL+KVI+YI REY ISHVTIQIER
Sbjct: 366 WAITVGKALFSCHVKVRPEAGDEMVLNKVIDYIWREYRISHVTIQIER 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6605616.12.9e-19886.79Metal tolerance protein A2, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
XP_023534426.12.0e-19686.53metal tolerance protein 1-like [Cucurbita pepo subsp. pepo] >XP_023534427.1 meta... [more]
XP_022958195.13.5e-19685.88metal tolerance protein 1-like [Cucurbita moschata] >XP_022958198.1 metal tolera... [more]
XP_022995642.11.1e-19485.42metal tolerance protein 1-like isoform X1 [Cucurbita maxima][more]
XP_022995643.11.9e-19485.62metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995644.1 met... [more]
Match NameE-valueIdentityDescription
Q9LXS11.2e-14665.90Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2[more]
Q9ZT631.5e-14669.12Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2[more]
Q688R12.7e-14368.25Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 ... [more]
Q9M2715.7e-10956.62Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1[more]
Q6DBM81.1e-8040.57Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H2S01.7e-19685.88metal tolerance protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459497 PE=... [more]
A0A6J1JZI05.4e-19585.42metal tolerance protein 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1JZI69.3e-19585.62metal tolerance protein 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A0A0KD362.5e-19284.68Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G080940 PE=4 SV=1[more]
A7L8D21.3e-19185.39Metal tolerance protein OS=Cucumis sativus OX=3659 GN=MTP PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G58810.28.4e-14865.90metal tolerance protein A2 [more]
AT2G46800.11.1e-14769.12zinc transporter of Arabidopsis thaliana [more]
AT2G46800.21.1e-14769.12zinc transporter of Arabidopsis thaliana [more]
AT3G58810.11.1e-14765.90metal tolerance protein A2 [more]
AT3G61940.14.1e-11056.62Cation efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002524Cation efflux proteinTIGRFAMTIGR01297TIGR01297coord: 66..445
e-value: 4.2E-97
score: 322.8
IPR002524Cation efflux proteinPFAMPF01545Cation_effluxcoord: 69..366
e-value: 5.2E-49
score: 166.7
IPR027469Cation efflux transmembrane domain superfamilyGENE3D1.20.1510.10Cation efflux protein transmembrane domaincoord: 65..365
e-value: 6.4E-47
score: 161.7
IPR027469Cation efflux transmembrane domain superfamilySUPERFAMILY161111Cation efflux protein transmembrane domain-likecoord: 63..367
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..298
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 196..298
NoneNo IPR availablePANTHERPTHR11562:SF88METAL TOLERANCE PROTEIN A1coord: 11..444
NoneNo IPR availablePANTHERPTHR11562CATION EFFLUX PROTEIN/ ZINC TRANSPORTERcoord: 11..444
IPR036837Cation efflux protein, cytoplasmic domain superfamilySUPERFAMILY160240Cation efflux protein cytoplasmic domain-likecoord: 372..443

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Sed0025819Sed0025819gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0025819.2-exonSed0025819.2-exon-LG01:66855255..66856854exon
Sed0025819.2-exonSed0025819.2-exon-LG01:66857363..66857475exon
Sed0025819.2-exonSed0025819.2-exon-LG01:66859318..66859493exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0025819.2-three_prime_utrSed0025819.2-three_prime_utr-LG01:66855255..66855546three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0025819.2-cdsSed0025819.2-cds-LG01:66855547..66856854CDS
Sed0025819.2-cdsSed0025819.2-cds-LG01:66857363..66857392CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0025819.2-five_prime_utrSed0025819.2-five_prime_utr-LG01:66857393..66857475five_prime_UTR
Sed0025819.2-five_prime_utrSed0025819.2-five_prime_utr-LG01:66859318..66859493five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Sed0025819.2Sed0025819.2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
biological_process GO:0071577 zinc ion transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005773 vacuole
molecular_function GO:0008324 cation transmembrane transporter activity
molecular_function GO:0005385 zinc ion transmembrane transporter activity