Sed0025357.1 (mRNA) Chayote v1

BLAST of Sed0025357.1 vs. NCBI nr
Match: XP_022158061.1 (beta-amyrin 28-oxidase [Momordica charantia])

HSP 1 Score: 872.8 bits (2254), Expect = 1.3e-249
Identity = 415/483 (85.92%), Postives = 445/483 (92.13%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGE 60
           MEH L+SLL+ F+S + + LF+LFYKHKS FSYPN+PPG IG PI+GESLEF STGWKGE
Sbjct: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60

Query: 61  PEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPS 120
           PEKFIFDRI K+KS IFKTSIVG PAAV CGP CNKFLFSNE+KLVTAWWPDSVNKIFPS
Sbjct: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 180
           STQT+S EEAKKLKKLLPQFLKPEAL RYIGIMD LA RHFA SWEN+++VL+FPLAK+F
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180

Query: 181 TFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLK 240
           TF+LACRLFVSVED AHVERLSGPFQHIAAGIISMPI+ PGTPFNR IKASNFIRKELL 
Sbjct: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240

Query: 241 IVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACT 300
           IV+QR  DLAEGKASPTQDILSHMLLTCDE+G +M E DIADKILGLLIGGHDTASAACT
Sbjct: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300

Query: 301 FIAKFLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FI KFLAELPHIYDGVYKEQM IAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPF 420
           AFREA+ DFVFNGFYIPKGWKLYWSSNSTHKN ++FP+PY FDPSRFEG GPAPYTFVPF
Sbjct: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPHKS 480
           GGGPRMCPGKEYARLEILVF+HNLVKRFKW K+LEKE IV DPMPIPQKGLP+RLFPHK+
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480

Query: 481 PSS 484
            S+
Sbjct: 481 VSA 483

BLAST of Sed0025357.1 vs. NCBI nr
Match: XP_038877759.1 (LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida])

HSP 1 Score: 852.8 bits (2202), Expect = 1.4e-243
Identity = 406/479 (84.76%), Postives = 441/479 (92.07%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGE 60
           MEH LISLL+ FL FLS+TLF+LF+ HKSLFSYPNVPPG IG+PILGESLEFLSTGWKGE
Sbjct: 1   MEHFLISLLIFFLFFLSLTLFILFHNHKSLFSYPNVPPGAIGLPILGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+ K+KS++FKTSIVGVPAA+ CG  CNKFLFSNE+KLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLHKYKSDVFKTSIVGVPAAIFCGSACNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 180
           STQT+SKEEAKKLKK LPQFLKPEALQRYIG+MD+LAQRHFA  WENREEVL+FPLAK+F
Sbjct: 121 STQTSSKEEAKKLKKFLPQFLKPEALQRYIGVMDELAQRHFASFWENREEVLVFPLAKSF 180

Query: 181 TFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLK 240
           TF+LACRLFVSVED  HV+RLSGPF+HIAAGIIS+PI+LPGTPFNRAIKAS FIRKEL+ 
Sbjct: 181 TFSLACRLFVSVEDEIHVKRLSGPFEHIAAGIISVPIDLPGTPFNRAIKASKFIRKELVA 240

Query: 241 IVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACT 300
           IV++R  DLAEGKAS TQDILSHMLLTCDE+G  M ESDIADKILGLLIGGHDTAS ACT
Sbjct: 241 IVKKRRWDLAEGKASATQDILSHMLLTCDENGVFMNESDIADKILGLLIGGHDTASVACT 300

Query: 301 FIAKFLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FI KFLAELPHIY GVY EQMEIAK KAPGE L WEDI+KMKYSWNV CEVLRLASP QG
Sbjct: 301 FIVKFLAELPHIYHGVYTEQMEIAKGKAPGEALKWEDIKKMKYSWNVVCEVLRLASPXQG 360

Query: 361 AFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPF 420
           AFREALTDFVFNGFYIPKGWKLYWS+NSTHK+ E+FPEPYKF+P+RFEG GP PYTFVPF
Sbjct: 361 AFREALTDFVFNGFYIPKGWKLYWSANSTHKSPEYFPEPYKFEPARFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPHK 480
           GGGPRMCPGKEYA+LEILVF+HNLVKRFKW KLLEKE I+ DPMPIPQKGLPIRLFPH+
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPMPIPQKGLPIRLFPHQ 479

BLAST of Sed0025357.1 vs. NCBI nr
Match: XP_022987538.1 (beta-amyrin 28-oxidase [Cucurbita maxima])

HSP 1 Score: 852.8 bits (2202), Expect = 1.4e-243
Identity = 409/484 (84.50%), Postives = 447/484 (92.36%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGE 60
           ME  LISLL+LFLSF+S+TLFVLFYKHKSLFSYPN PPG IG+PI+GESLEFLSTGWKGE
Sbjct: 1   MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+ K+KS+IFKTSIVGVPAA+ CGPVCNKFLFSNE+KLVT+WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 180
           ST+ NSKE A +LKKLLP+FLKPEALQRYIG+MD+LAQRHFA SWENRE VL+FPLAK+F
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKSF 180

Query: 181 TFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLK 240
           TF+LACRLF+S+ED  HVERLS PFQ+IAAGIISMPI+LPGTPFNRAIKASNF+RKELL 
Sbjct: 181 TFSLACRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACT 300
           IVRQR +DLAEGKASPTQDILSHMLLTCDE G +M ES IAD ILGLLIGGHDTASAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACT 300

Query: 301 FIAKFLAELPHIYDGVYKEQMEI--AKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPL 360
           FI K+LAELP IYD VY+EQMEI  AKAKA GELL WEDIQKMKYSWNVACEVLRLASPL
Sbjct: 301 FIVKYLAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPL 360

Query: 361 QGAFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFV 420
           QGAFREA+ DFVFNGF IPKGWKLYWSSNSTHKN  +FPEPYKFDPSRFEG GPAPYTFV
Sbjct: 361 QGAFREAINDFVFNGFNIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420

Query: 421 PFGGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPH 480
           PFGGGPRMCPGKEYA+LEILVF+HNLVKRFKW KLLEKE I+ DP+PIP+KGLP+RLFPH
Sbjct: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLEKEKIIVDPLPIPEKGLPVRLFPH 480

Query: 481 KSPS 483
           K+ S
Sbjct: 481 KTLS 484

BLAST of Sed0025357.1 vs. NCBI nr
Match: XP_023516848.1 (beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852.4 bits (2201), Expect = 1.9e-243
Identity = 403/479 (84.13%), Postives = 442/479 (92.28%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGE 60
           M   LISLL+LFLSF+S+TLFVLFYKHKSLFSYPN PPG IG+PI+GESLEFLSTGWKGE
Sbjct: 1   MADFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+ K+KS+IFKTSIVGVPAA+ CGP CNKFLFSNE+KLVT+WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 180
           ST+ NSKE A +LKKLLP+FLKPEALQRYIG+MD+LAQRHFA SWENRE+VL+FPLAK+F
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 181 TFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLK 240
           TF+LACRLFVS+ED  HVERLS PFQ+IAAGIISMPI+LPGTPFNRAIKASNF+RKELL 
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACT 300
           IVRQR +DLAEGKASPTQDILSHMLLTCDE G +M ES IAD ILGLLIGGHDT SAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 301 FIAKFLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FI K+LAELP IYD VY+EQMEIAKAK  GELL WEDIQKMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVKYLAELPQIYDSVYREQMEIAKAKGAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPF 420
           AFRE + DFVFNGFYIPKGWKLYWSSNSTHKN  +FPEPYKFDPSRFEG GPAPYTFVPF
Sbjct: 361 AFREPINDFVFNGFYIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPHK 480
           GGGPRMCPGKEYA+LEILVF+HNLVKRFKW KL++KE I+ DP+PIP+KGLP+RLFPHK
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLMDKEKIIVDPLPIPEKGLPVRLFPHK 479

BLAST of Sed0025357.1 vs. NCBI nr
Match: KAG6590124.1 (Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023791.1 hypothetical protein SDJN02_14817 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 850.5 bits (2196), Expect = 7.1e-243
Identity = 403/479 (84.13%), Postives = 441/479 (92.07%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGE 60
           M   LISLL+LFLS +S+TLFVLFYKHKSLFSYPN PPG IG+PI+GESLEFLSTGWKGE
Sbjct: 1   MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+ K+KS+IFKTSIVGVPAA+ CGP CNKFLFSNE+KLVT+WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 180
           ST+ NSKE A +LKKLLP+FLKPEALQRYIG+MD+LAQRHFA SWENRE+VL+FPLAK+F
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 181 TFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLK 240
           TF+LACRLFVS+ED  HVERLS PFQ IAAGIISMPI+LPGTPFNRAIKASNF+RKELL 
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACT 300
           IVRQR +DLAEGKASPTQDILSHMLLTCDE G +M ES IAD ILGLLIGGHDT SAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 301 FIAKFLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FI K+LAELP IYDGVY+EQMEIAKAKA GELL WEDIQKMKYSWNV CEVLRLASPLQG
Sbjct: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360

Query: 361 AFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPF 420
           AFREA+  FVFNGFYIPKGWK+YWSSNSTHKN  +FPEPYKFDPSRFEG GPAPYTFVPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPHK 480
           GGGPRMCPGKEYA+LEILVF+HNLVKRFKW KLL+KE I+ DP+PIP+KGLP+RLFPHK
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHK 479

BLAST of Sed0025357.1 vs. ExPASy Swiss-Prot
Match: A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)

HSP 1 Score: 755.7 bits (1950), Expect = 3.1e-217
Identity = 356/479 (74.32%), Postives = 417/479 (87.06%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGE 60
           ME   +SLL++ +  +SI L++L YKH+S F+ PN+PPG IG P++GESLEFLSTGWKG 
Sbjct: 1   MEVFFLSLLLICVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 61  PEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPS 120
           PEKFIFDRI+K+ SE+FKTS++G PAAV  G   NKFLFSNE+KLV AWWP SV+K+FPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 180
           STQT+SKEEAKK++KLLPQFLKPEALQRY GIMD +AQRHFA SW+NR+EV++FPLAK F
Sbjct: 121 STQTSSKEEAKKMRKLLPQFLKPEALQRYTGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180

Query: 181 TFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLK 240
           TF LACRLF+S+EDPAHV +   PF  +A+G+I++PI+LPGTPF+RAIKASNFIRKEL  
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITIPIDLPGTPFHRAIKASNFIRKELRA 240

Query: 241 IVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACT 300
           I++QR  DLAE KAS TQDILSHMLL  DE G HM E  IADKILGLLIGGHDTAS+A T
Sbjct: 241 IIKQRKIDLAESKASKTQDILSHMLLATDEDGCHMNEMSIADKILGLLIGGHDTASSAIT 300

Query: 301 FIAKFLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           F+ K++AELPHIY+ VYKEQMEIA +KAPGELLNW+D+QKM+YSWNVACEV+RLA PLQG
Sbjct: 301 FLVKYMAELPHIYEKVYKEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360

Query: 361 AFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPF 420
           AFREA+TDFVFNGF IPKGWKLYWS+NSTHK+ E FP+P KFDP+RFEG GPAPYTFVPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSLECFPQPEKFDPTRFEGAGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPHK 480
           GGGPRMCPGKEYARLEIL+F+HNLVKRFKW+KLL  E I+ DPMP+P KGLP+RL PHK
Sbjct: 421 GGGPRMCPGKEYARLEILIFMHNLVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPHK 479

BLAST of Sed0025357.1 vs. ExPASy Swiss-Prot
Match: F6H9N6 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 5.3e-217
Identity = 356/479 (74.32%), Postives = 417/479 (87.06%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGE 60
           ME   +SLL++F+  +SI L +LFYKH+S F+ PN+PPG IG P++GESLEFLSTGWKG 
Sbjct: 1   MEVFFLSLLLIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 61  PEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPS 120
           PEKFIFDRI+K+ SE+FKTS++G PAAV  G   NKFLFSNE+KLV AWWP SV+K+FPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 180
           STQT+SKEEAKK++KLLPQF KPEALQRYIGIMD +AQRHFA SW+NR+EV++FPLAK F
Sbjct: 121 STQTSSKEEAKKMRKLLPQFFKPEALQRYIGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180

Query: 181 TFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLK 240
           TF LACRLF+S+EDPAHV +   PF  +A+G+I++PI+LPGTPF+RAIKASNFIRKEL  
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITVPIDLPGTPFHRAIKASNFIRKELRA 240

Query: 241 IVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACT 300
           I++QR  DLAEGKAS  QDILSHMLL  DE G HM E +IADKILGLLIGGHDTASAA T
Sbjct: 241 IIKQRKIDLAEGKASQNQDILSHMLLATDEDGCHMNEMEIADKILGLLIGGHDTASAAIT 300

Query: 301 FIAKFLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           F+ K++AELPHIY+ VY+EQMEIA +KAPGELLNW+D+QKM+YSWNVACEV+RLA PLQG
Sbjct: 301 FLIKYMAELPHIYEKVYEEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360

Query: 361 AFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPF 420
           AFREA+TDFVFNGF IPKGWKLYWS+NSTHK+ E FP+P  FDP+RFEG GPAPYTFVPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSPECFPQPENFDPTRFEGDGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPHK 480
           GGGPRMCPGKEYARLEILVF+HN+VKRFKW+KLL  E I+ DPMP+P KGLP+RL P K
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNVVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPRK 479

BLAST of Sed0025357.1 vs. ExPASy Swiss-Prot
Match: Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 2.3e-212
Identity = 349/476 (73.32%), Postives = 407/476 (85.50%), Query Frame = 0

Query: 6   ISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGEPEKFI 65
           +SLL+LF++F+S++LF +FYK KS     N+PPG +G PI+GESLEFLSTGWKG PEKFI
Sbjct: 7   LSLLLLFVTFISLSLFFIFYKQKSPL---NLPPGKMGYPIIGESLEFLSTGWKGHPEKFI 66

Query: 66  FDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFP-SSTQT 125
           FDR+ K+ SE+FKTSIVG    V CG   NKFLFSNE+KLVTAWWPDSVNKIFP +S  +
Sbjct: 67  FDRMRKYSSELFKTSIVGESTVVCCGAASNKFLFSNENKLVTAWWPDSVNKIFPTTSLDS 126

Query: 126 NSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTFTFAL 185
           N KEE+ K++KLLPQF KPEALQRY+G+MD +AQRHF   W+N+ E+ ++PLAK +TF L
Sbjct: 127 NLKEESIKMRKLLPQFFKPEALQRYVGVMDVIAQRHFVTHWDNKNEITVYPLAKRYTFLL 186

Query: 186 ACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLKIVRQ 245
           ACRLF+SVED  HV + S PFQ IAAGIIS+PI+LPGTPFN+AIKASNFIRKEL+KI++Q
Sbjct: 187 ACRLFMSVEDENHVAKFSDPFQLIAAGIISLPIDLPGTPFNKAIKASNFIRKELIKIIKQ 246

Query: 246 RNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACTFIAK 305
           R  DLAEG ASPTQDILSHMLLT DE+G  M E +IADKILGLLIGGHDTAS ACTF+ K
Sbjct: 247 RRVDLAEGTASPTQDILSHMLLTSDENGKSMNELNIADKILGLLIGGHDTASVACTFLVK 306

Query: 306 FLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQGAFRE 365
           +L ELPHIYD VY+EQMEIAK+K  GELLNW+D++KMKYSWNVACEV+RL+ PLQG FRE
Sbjct: 307 YLGELPHIYDKVYQEQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGGFRE 366

Query: 366 ALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPFGGGP 425
           A+TDF+FNGF IPKGWKLYWS+NSTHKN E FP P KFDP+RFEG GPAPYTFVPFGGGP
Sbjct: 367 AITDFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGPAPYTFVPFGGGP 426

Query: 426 RMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPHKS 481
           RMCPGKEYARLEILVF+HNLVKRFKWEK++  E I+ DP PIP K LPIRL+PHK+
Sbjct: 427 RMCPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYPHKA 479

BLAST of Sed0025357.1 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 1.9e-211
Identity = 348/481 (72.35%), Postives = 406/481 (84.41%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKS-LFSYPNVPPGTIGIPILGESLEFLSTGWKG 60
           M+   + LL LF+ F+S++ + LFYK KS   S   +PPG  G P++GES EFLSTGWKG
Sbjct: 1   MQLFYVPLLSLFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKG 60

Query: 61  EPEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFP 120
            PEKFIFDR+ K+ S +FKTS+ G PAA  CG  CNKFLFSNE+KLV AWWPDSVNK+FP
Sbjct: 61  YPEKFIFDRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFP 120

Query: 121 SSTQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKT 180
           SSTQT+SKEEA K++KLLP FLKPEALQRY+G+MDQ+A +HF   WEN++EVL+FPLAK 
Sbjct: 121 SSTQTSSKEEAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKN 180

Query: 181 FTFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELL 240
           +TF +AC++FVSVE+P  V +L  PF  IA+GIIS+PI+LPGTPFN AIK+S  +R +L+
Sbjct: 181 YTFWIACKVFVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKLM 240

Query: 241 KIVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAAC 300
            I++QR  DL EGKASPTQDILSHMLLT DE+G  M E DIADKILGLLIGGHDTAS+AC
Sbjct: 241 GIIKQRKTDLGEGKASPTQDILSHMLLTSDENGKFMTEGDIADKILGLLIGGHDTASSAC 300

Query: 301 TFIAKFLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQ 360
           TF+ KFLAELP IY+GVYKEQMEIAK+K  GELLNWEDIQKMKYSWNVACEVLRLA PLQ
Sbjct: 301 TFVVKFLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQ 360

Query: 361 GAFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVP 420
           GAFREALTDF +NGF IPKGWKLYWS+NSTH+N E FPEP KFDPSRFEG GP PY+FVP
Sbjct: 361 GAFREALTDFSYNGFSIPKGWKLYWSANSTHRNSEVFPEPLKFDPSRFEGAGPPPYSFVP 420

Query: 421 FGGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPHK 480
           FGGGPRMCPGKEYARLEILVF+H++VKRFKWEK++  E IV +PMPIP KGLP+RLFPHK
Sbjct: 421 FGGGPRMCPGKEYARLEILVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFPHK 480

BLAST of Sed0025357.1 vs. ExPASy Swiss-Prot
Match: A0A3Q7HBJ5 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A44 PE=1 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 1.7e-207
Identity = 341/478 (71.34%), Postives = 403/478 (84.31%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGE 60
           ME L + L+ +F+  +S+   +L YK K   S   +PPG  G P+ GESLEFLS+GWKG 
Sbjct: 1   MELLYVCLVCVFVFLVSL---LLLYKKK---SGEGLPPGKTGWPVFGESLEFLSSGWKGH 60

Query: 61  PEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+ K+ S +FKT ++G  AAV CG   NKFLFSNE+KLV AWWP+SVNK+FPS
Sbjct: 61  PEKFIFDRVAKYSSSVFKTHLLGEEAAVFCGASANKFLFSNENKLVQAWWPNSVNKVFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 180
           STQT+SKEEA K++K+LP F KPEALQRY+GIMD + QRHFA  WEN+E+V++FPL K +
Sbjct: 121 STQTSSKEEAIKMRKMLPNFFKPEALQRYVGIMDHITQRHFATGWENKEQVVVFPLTKRY 180

Query: 181 TFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLK 240
           TF LACRLF+SVEDP HV + + PF  +A+G+IS+PI+LPGTPFNRAIKASNFIRKEL++
Sbjct: 181 TFWLACRLFLSVEDPKHVAKFADPFDVLASGLISIPIDLPGTPFNRAIKASNFIRKELVR 240

Query: 241 IVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACT 300
           I++QR  DL EGK S TQDILSHMLLTCDE+G  + + DIADKILGLLIGGHDTAS+AC+
Sbjct: 241 IIKQRKIDLGEGKVSSTQDILSHMLLTCDENGKFLGDLDIADKILGLLIGGHDTASSACS 300

Query: 301 FIAKFLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FI K+LAELPHIY  VY EQMEIAK+K PGELL WEDIQKMKYSWNVACEVLRLA PLQG
Sbjct: 301 FIVKYLAELPHIYQRVYTEQMEIAKSKGPGELLRWEDIQKMKYSWNVACEVLRLAPPLQG 360

Query: 361 AFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPF 420
           AFREAL+DF+FNGFYIPKGWK+YWS+NSTHK  EFFP+P KFDPSRFEG GPAPYTFVPF
Sbjct: 361 AFREALSDFMFNGFYIPKGWKIYWSANSTHKREEFFPDPEKFDPSRFEGSGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPH 479
           GGGPRMCPGKEYARLEILVF+H+LVKRFK+EK++  E I+ +PMPIP  GLP+RL+PH
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHHLVKRFKFEKIIPHEKIIVNPMPIPANGLPLRLYPH 472

BLAST of Sed0025357.1 vs. ExPASy TrEMBL
Match: A0A6J1DZX7 (beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 6.4e-250
Identity = 415/483 (85.92%), Postives = 445/483 (92.13%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGE 60
           MEH L+SLL+ F+S + + LF+LFYKHKS FSYPN+PPG IG PI+GESLEF STGWKGE
Sbjct: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60

Query: 61  PEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPS 120
           PEKFIFDRI K+KS IFKTSIVG PAAV CGP CNKFLFSNE+KLVTAWWPDSVNKIFPS
Sbjct: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 180
           STQT+S EEAKKLKKLLPQFLKPEAL RYIGIMD LA RHFA SWEN+++VL+FPLAK+F
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180

Query: 181 TFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLK 240
           TF+LACRLFVSVED AHVERLSGPFQHIAAGIISMPI+ PGTPFNR IKASNFIRKELL 
Sbjct: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240

Query: 241 IVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACT 300
           IV+QR  DLAEGKASPTQDILSHMLLTCDE+G +M E DIADKILGLLIGGHDTASAACT
Sbjct: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300

Query: 301 FIAKFLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FI KFLAELPHIYDGVYKEQM IAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPF 420
           AFREA+ DFVFNGFYIPKGWKLYWSSNSTHKN ++FP+PY FDPSRFEG GPAPYTFVPF
Sbjct: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPHKS 480
           GGGPRMCPGKEYARLEILVF+HNLVKRFKW K+LEKE IV DPMPIPQKGLP+RLFPHK+
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480

Query: 481 PSS 484
            S+
Sbjct: 481 VSA 483

BLAST of Sed0025357.1 vs. ExPASy TrEMBL
Match: A0A6J1JJQ7 (beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 6.9e-244
Identity = 409/484 (84.50%), Postives = 447/484 (92.36%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGE 60
           ME  LISLL+LFLSF+S+TLFVLFYKHKSLFSYPN PPG IG+PI+GESLEFLSTGWKGE
Sbjct: 1   MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+ K+KS+IFKTSIVGVPAA+ CGPVCNKFLFSNE+KLVT+WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 180
           ST+ NSKE A +LKKLLP+FLKPEALQRYIG+MD+LAQRHFA SWENRE VL+FPLAK+F
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKSF 180

Query: 181 TFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLK 240
           TF+LACRLF+S+ED  HVERLS PFQ+IAAGIISMPI+LPGTPFNRAIKASNF+RKELL 
Sbjct: 181 TFSLACRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACT 300
           IVRQR +DLAEGKASPTQDILSHMLLTCDE G +M ES IAD ILGLLIGGHDTASAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACT 300

Query: 301 FIAKFLAELPHIYDGVYKEQMEI--AKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPL 360
           FI K+LAELP IYD VY+EQMEI  AKAKA GELL WEDIQKMKYSWNVACEVLRLASPL
Sbjct: 301 FIVKYLAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPL 360

Query: 361 QGAFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFV 420
           QGAFREA+ DFVFNGF IPKGWKLYWSSNSTHKN  +FPEPYKFDPSRFEG GPAPYTFV
Sbjct: 361 QGAFREAINDFVFNGFNIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420

Query: 421 PFGGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPH 480
           PFGGGPRMCPGKEYA+LEILVF+HNLVKRFKW KLLEKE I+ DP+PIP+KGLP+RLFPH
Sbjct: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLEKEKIIVDPLPIPEKGLPVRLFPH 480

Query: 481 KSPS 483
           K+ S
Sbjct: 481 KTLS 484

BLAST of Sed0025357.1 vs. ExPASy TrEMBL
Match: A0A6J1H8T4 (beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.3e-242
Identity = 403/482 (83.61%), Postives = 442/482 (91.70%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGE 60
           M   LISLL+LFLS +S+TLFVLFYKHKSLFSYPN PPG IG+PI+GESLEFLSTGWKGE
Sbjct: 1   MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+ K+KS+IFKTSIVGVPAA+ CGP CNKFLFSNE+KLVT+WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 180
           ST+ NSKE A +LKKLLP+FLKPEALQRYIG+MD+LAQRHFA SWENRE+VL+FPLAK+F
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 181 TFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLK 240
           TF+LACRLFVS+ED  HVERLS PFQ IAAGIISMPI+LPGTPFNRAIKASNF+RKELL 
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACT 300
           IVRQR +DLAEGKASPTQDILSHMLLTCDE G +M ES IAD ILGLLIGGHDT SAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 301 FIAKFLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FI  +LAELP IYDGVY+EQMEIAKAKA GELL WEDIQKMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVNYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPF 420
           AFREA+  FVFNGFYIPKGWK+YWSSNSTHKN  +FPEPYKFDPSRFEG GPAPYTFVPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPHKS 480
           GGGPRMCPGKEYA+LEILVF+HNLVKRFKW KLL+KE I+ DP+PIP+KGLP+RLFPH +
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHTT 480

Query: 481 PS 483
            S
Sbjct: 481 LS 482

BLAST of Sed0025357.1 vs. ExPASy TrEMBL
Match: A0A1S3B8H4 (beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.6e-240
Identity = 397/479 (82.88%), Postives = 438/479 (91.44%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGE 60
           MEH LISLL+L   FLS+TLF+LF+ HKSLFSYPN PPG IG+PILGESLEFLS+GW+G 
Sbjct: 1   MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60

Query: 61  PEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+ K+KS++FKTSIVGVPAA+ CGP+CNKFLFSNE+KLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 180
           +TQT+SKEEAKKLKKLLPQFLKPEALQRYIGIMD+LA+RHF   W+NREEVL+FPLAK+F
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 181 TFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLK 240
           TF+LACRLF+SVED  HVERLSGPF+HIAAGIISMPI+LPGTPFNRAIKAS FIRKE++ 
Sbjct: 181 TFSLACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 241 IVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACT 300
           IVRQR  DLAEGKA  TQDILSHMLLTCDE+G +M ESDI DKILGLLIGGHDTAS ACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 301 FIAKFLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FI KFLAELPHIYD VY EQMEIA+AKA GE L WEDI+KMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPF 420
           AFREAL DFVFNGF+IPKGWKLYWS+NSTHKN E+FPEPYKF+P RFEG GP PYTFVPF
Sbjct: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPHK 480
           GGGPRMCPGKEYA+LEILVF+HNLVKRFKW KLLE E I+ +PMPIPQKGLP+RLFPH+
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQ 479

BLAST of Sed0025357.1 vs. ExPASy TrEMBL
Match: A0A0A0LXN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 5.1e-239
Identity = 394/479 (82.25%), Postives = 438/479 (91.44%), Query Frame = 0

Query: 1   MEHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGE 60
           ME  LISLL+L   FLS+TLF+LF+ HKSLFSYPN PPG IG+PILGES+EFLS+GWKG 
Sbjct: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60

Query: 61  PEKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPS 120
           PEKFIFDR+ K+KS++FKTSIVGVPAA+ CGP+CNKFLFSNE+KLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 180
           +TQT++KEEAKKLKKLLPQFLKPEALQRYIGIMD+LA+RHF   W+NREEVL+FPLAK+F
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 181 TFALACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLK 240
           TF++ACRLF+SVED  HVERLSGPF+HIAAGIISMPI+LPGTPFNRAIKAS FIRKE++ 
Sbjct: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 241 IVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACT 300
           IVRQR  DLAEGKA  TQDILSHMLLTCDE+G +M ESDI DKILGLLIGGHDTAS ACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 301 FIAKFLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
           FI KFLAELPHIYD VY EQMEIA+AKA GE L WEDI+KMKYSWNVACEVLR+ASPLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360

Query: 361 AFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPF 420
           AFREAL+DFVFNGF+IPKGWKLYWS+NSTHKN E+FPEPYKFDP RFEG GP PYTFVPF
Sbjct: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPHK 480
           GGGPRMCPGKEYA+LEILVF+HNLVKRFKW KLLE E I+ +PMPIPQKGLP+RLFPH+
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQ 479

BLAST of Sed0025357.1 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 626.3 bits (1614), Expect = 2.0e-179
Identity = 294/474 (62.03%), Postives = 366/474 (77.22%), Query Frame = 0

Query: 8   LLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGEPEKFIFD 67
           +++LFLS + ++L +L  KH S FSYPN+PPG  G+P++GES  FLS G +G PEKFI D
Sbjct: 6   MIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEKFITD 65

Query: 68  RITKFKSE---IFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPSSTQT 127
           R+ +F S    +FKT + G P AV+ G   NKFLF+NE+KLV +WWPDSVNKIFPSS QT
Sbjct: 66  RVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFPSSMQT 125

Query: 128 NSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTFTFAL 187
           +SKEEA+KL+ LL QF+KPEAL+RY+G+MD++AQRHF   W N+++V++FPL K FTF++
Sbjct: 126 SSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKFTFSI 185

Query: 188 ACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLKIVRQ 247
           ACR F+S+EDPA V +L   F  +A GI S+PI+LPGT FNRAIKAS  +RKE+  IVRQ
Sbjct: 186 ACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSAIVRQ 245

Query: 248 RNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACTFIAK 307
           R  +L  GKA    DILSHML+   E+    K+ D+ADKI+GLLIGGHDTAS  CTF+  
Sbjct: 246 RKEELKAGKALEEHDILSHMLMNIGET----KDEDLADKIIGLLIGGHDTASIVCTFVVN 305

Query: 308 FLAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQGAFRE 367
           +LAE PH+Y  V +EQ EI K K   E L WEDI+KM+YSWNVACEV+R+  PL G FRE
Sbjct: 306 YLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSGTFRE 365

Query: 368 ALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPFGGGP 427
           A+  F F GFYIPKGWKLYWS+ +TH N ++FPEP +F+P+RFEG GP PYT+VPFGGGP
Sbjct: 366 AIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVPFGGGP 425

Query: 428 RMCPGKEYARLEILVFIHNLVKRFKWEKLLEKE-MIVCDPMPIPQKGLPIRLFP 478
           RMCPGKEYARLEIL+F+HNLV RFKWEK+   E  IV DP+PIP KGLPIR+FP
Sbjct: 426 RMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFP 475

BLAST of Sed0025357.1 vs. TAIR 10
Match: AT5G36140.1 (cytochrome P450, family 716, subfamily A, polypeptide 2 )

HSP 1 Score: 355.5 bits (911), Expect = 6.6e-98
Identity = 174/320 (54.37%), Postives = 230/320 (71.88%), Query Frame = 0

Query: 4   LLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGEPEK 63
           + ++++ LF+S +   L     KH S F YPN+PPG IG P++GE+L FLS G +G PEK
Sbjct: 1   MYLTIIFLFISSIIFPLLFFLGKHLSNFRYPNLPPGKIGFPLIGETLSFLSAGRQGHPEK 60

Query: 64  FIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNKIFPSSTQ 123
           F+ DR+  F S IFKT + G P AV+ G   NKFLF+NE+KLV +WWPDSVNKIFPSSTQ
Sbjct: 61  FVTDRVRHFSSGIFKTHLFGSPFAVVTGASGNKFLFTNENKLVISWWPDSVNKIFPSSTQ 120

Query: 124 TNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTFTFA 183
           T+SKEEA K + LL   +KPEAL+RY+G+MD++AQ+HF   W N++++++FPL K FTF+
Sbjct: 121 TSSKEEAIKTRMLLMPSMKPEALRRYVGVMDEIAQKHFETEWANQDQLIVFPLTKKFTFS 180

Query: 184 LACRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLKIVR 243
           +ACRLF+S++D   V +L  PF  +  G+ S+PI+LPGT FNRAIKAS  + KE+  I+R
Sbjct: 181 IACRLFLSMDDLERVRKLEEPFTTVMTGVFSIPIDLPGTRFNRAIKASRLLSKEVSTIIR 240

Query: 244 QRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACTFIA 303
           QR  +L  GK S  QDILSHML+   E+    K+ D+ADKI+ LLIGGHDT S  CTF+ 
Sbjct: 241 QRKEELKAGKVSVEQDILSHMLMNIGET----KDEDLADKIIALLIGGHDTTSIVCTFVV 300

Query: 304 KFLAELPHIYDGVYKEQMEI 324
            +LAE PHIY  V  E M+I
Sbjct: 301 NYLAEFPHIYQRVL-EGMQI 315

BLAST of Sed0025357.1 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 270.4 bits (690), Expect = 2.8e-72
Identity = 160/475 (33.68%), Postives = 247/475 (52.00%), Query Frame = 0

Query: 16  LSITLFVLFYKH-----KSLFSYPNVPPGTIGIPILGESLEFLSTGWKGEP-EKFIFDRI 75
           +S TLF    KH     K +     + PG +G+P +GE+++F          E F+  RI
Sbjct: 20  ISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRI 79

Query: 76  TKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWP-DSVNKIFPSSTQTNSKE 135
            K    IFKT I+G P  V+ G   N+ + SNE  LV + WP  SV  +  +       E
Sbjct: 80  IK-HGNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGE 139

Query: 136 EAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAK--TFTFALAC 195
           + + L+ ++   L    L+  I  +    + H    W  +EE+ ++  AK  TFT    C
Sbjct: 140 KHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFEC 199

Query: 196 RLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELLKIVRQRN 255
              + VE       +   F+ +  G+ ++P+  P + F RA KA   I   L+  VR++ 
Sbjct: 200 LYGIKVE-----IGMLEVFERVLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKR 259

Query: 256 ADL-AEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAACTFIAKF 315
            ++  EG   P   + S ++    +    + E ++ D ++ L+   HDT S A +   K 
Sbjct: 260 REMEKEGAEKPNTTLFSRLVEELIKG--VITEEEVVDNMVLLVFAAHDTTSYAMSMTFKM 319

Query: 316 LAELPHIYDGVYKEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQGAFREA 375
           LA+ P   D + +E  +I   K  GE L  ED++KMKYSW V  E +RL+ P+ G+FR+A
Sbjct: 320 LAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKA 379

Query: 376 LTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFVPFGGGPR 435
           + D  + G+ IPKGWK+ W++  TH N E F +P  FDP+RF+ K    YT++PFGGGPR
Sbjct: 380 VADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFD-KPIQAYTYLPFGGGPR 439

Query: 436 MCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFPHKS 481
           +C G + A++ ILVF+H +V  F W  +   E I  DP+P P  G+PI++ P  S
Sbjct: 440 LCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISPKVS 485

BLAST of Sed0025357.1 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 261.2 bits (666), Expect = 1.7e-69
Identity = 156/479 (32.57%), Postives = 260/479 (54.28%), Query Frame = 0

Query: 2   EHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGEP 61
           E  LI+L ++ +  + +  + L +K + L     +PPG++G+P +GE+L      +   P
Sbjct: 19  EPALITLTIVVVVVVLLFKWWLHWKEQRL----RLPPGSMGLPYIGETLRL----YTENP 78

Query: 62  EKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNK-IFPS 121
             F   R  K+  +IFKT I+G P  ++  P   + +  ++  L    +P S  + I P 
Sbjct: 79  NSFFATRQNKY-GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPE 138

Query: 122 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 181
           +   +       LK+L+     P AL+  +  ++ L  +  + SW +++ +      K +
Sbjct: 139 ALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLS-SWTSQKSINTLEYMKRY 198

Query: 182 TFALA-CRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELL 241
            F +A    F   E+P  ++ +   +Q +  G  SMP++LPGT F++++KA   + +EL 
Sbjct: 199 AFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELR 258

Query: 242 KIVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAAC 301
           K++ +R  +  E        +L  +L   D+  + + +S IAD I+G++    DT ++  
Sbjct: 259 KVIEKRRENGRE-----EGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVL 318

Query: 302 TFIAKFLAELPHIYDGVYKEQMEI-AKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPL 361
           T++ K+L + P++   V +EQ  I  K K     ++WED +KM  +  V  E LR AS L
Sbjct: 319 TWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVL 378

Query: 362 QGAFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFV 421
              FREA+ D  ++G+ IPKGWK+       H + EFFP+P KFDPSRFE   P PYT++
Sbjct: 379 SFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYM 438

Query: 422 PFGGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFP 478
           PFG G   CPG E A+LE+L+ +H+L   F+WE + ++E I   P P+P+KGLPIR+ P
Sbjct: 439 PFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

BLAST of Sed0025357.1 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 261.2 bits (666), Expect = 1.7e-69
Identity = 156/479 (32.57%), Postives = 260/479 (54.28%), Query Frame = 0

Query: 2   EHLLISLLVLFLSFLSITLFVLFYKHKSLFSYPNVPPGTIGIPILGESLEFLSTGWKGEP 61
           E  LI+L ++ +  + +  + L +K + L     +PPG++G+P +GE+L      +   P
Sbjct: 19  EPALITLTIVVVVVVLLFKWWLHWKEQRL----RLPPGSMGLPYIGETLRL----YTENP 78

Query: 62  EKFIFDRITKFKSEIFKTSIVGVPAAVLCGPVCNKFLFSNEDKLVTAWWPDSVNK-IFPS 121
             F   R  K+  +IFKT I+G P  ++  P   + +  ++  L    +P S  + I P 
Sbjct: 79  NSFFATRQNKY-GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPE 138

Query: 122 STQTNSKEEAKKLKKLLPQFLKPEALQRYIGIMDQLAQRHFAHSWENREEVLMFPLAKTF 181
           +   +       LK+L+     P AL+  +  ++ L  +  + SW +++ +      K +
Sbjct: 139 ALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLS-SWTSQKSINTLEYMKRY 198

Query: 182 TFALA-CRLFVSVEDPAHVERLSGPFQHIAAGIISMPINLPGTPFNRAIKASNFIRKELL 241
            F +A    F   E+P  ++ +   +Q +  G  SMP++LPGT F++++KA   + +EL 
Sbjct: 199 AFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELR 258

Query: 242 KIVRQRNADLAEGKASPTQDILSHMLLTCDESGHHMKESDIADKILGLLIGGHDTASAAC 301
           K++ +R  +  E        +L  +L   D+  + + +S IAD I+G++    DT ++  
Sbjct: 259 KVIEKRRENGRE-----EGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVL 318

Query: 302 TFIAKFLAELPHIYDGVYKEQMEI-AKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPL 361
           T++ K+L + P++   V +EQ  I  K K     ++WED +KM  +  V  E LR AS L
Sbjct: 319 TWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVL 378

Query: 362 QGAFREALTDFVFNGFYIPKGWKLYWSSNSTHKNGEFFPEPYKFDPSRFEGKGPAPYTFV 421
              FREA+ D  ++G+ IPKGWK+       H + EFFP+P KFDPSRFE   P PYT++
Sbjct: 379 SFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYM 438

Query: 422 PFGGGPRMCPGKEYARLEILVFIHNLVKRFKWEKLLEKEMIVCDPMPIPQKGLPIRLFP 478
           PFG G   CPG E A+LE+L+ +H+L   F+WE + ++E I   P P+P+KGLPIR+ P
Sbjct: 439 PFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481