Sed0023145.1 (mRNA) Chayote v1

Overview
NameSed0023145.1
TypemRNA
OrganismSechium edule (Chayote v1)
DescriptionE3 ubiquitin-protein ligase CHIP-like
LocationLG05: 1285891 .. 1290580 (+)
Sequence length1342
RNA-Seq ExpressionSed0023145.1
SyntenySed0023145.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTGTTCAATTTTTTAAACCTGGAAACGTAAATTTTCGGGTGGAAATTTGTTGAACCCTCAATCACTTCGTTCTGTTCTTCGTTTCCCACTTTTCTCTGAATCAAAATTTCTTTCTTCTTCTTCTTCTTCTTCAATTGGGAAATCATTCTCTGAAATCCCATTTATGGAAATTTCTCTAACTTCGTAATTTCTTGACCTAAACAATTCCATTGATGGGGGAGGGCTTGTCTGACCCCGCAAAGCAGGCCGAGCTGCTCAGAAAAGATGGAAACCTCTATTTCCAGAAGGGCCGAATTGGAGCCGCCATTGATGCCTACACCGAGGTTTCTATTTCTTGAATTCTTGTTTCTGTTTCCTATCTGGGTTTCCTCTGAATTTGCGTTTGATTGTATTAGAATCGAATTATTTGATTGATGCCGCAAGATTACTTAGTTGGGATTACTTAGGCGTGTTCTTGCGAAACGAAATGTGTAATTAGGTTTCCATTTCCGTACTTAGTTGGGATTATTTTGTAATAGGTTTTTATGATACTAACTCTCAACCCTAGTCCCAGCACAGTGACATTAGGGTGATTTTGCTTTCTTTAACACTTTTGTTGTTTGTGTTTCGAAGGCAATTACGTTGTGCCCGAATGTTCCTGTGTATTTGACGAACCGTGCTTTGTGTCATCGCAAGAGGAAGTATGTAGGGCACATATCTTTATTCCTTTCCTTATTTGGGAGACAAGTGAGAATTCAGAATTTATTGTTTAACTCTATTTCCTCTTTATTCACTCGTCTTGTTTATGGTGTATAGTGATTGGAAGAAGGTGGAGGAGGACTGTCGGAGAGCTATTCAGCTCGATAGCAACTCTGTCAAGGTATTGCTGTGATGTCTTCTTATACAAACCTTGTGAATTTGATTGTTTCTTAAGAGTCTAATAGTAATGCTTATGTGCACACGGATGACAGAACATTGCATGACATGGTACTTCATTTTACAAAGATCTAGGACACTGTCCACGGACACGCCAAGGATGTATTTATTTATTTGTTTTTTTTTTTTGAATTCAACGAAATTTGAGGTTGAGGGATTCGAACTTCTGACATCTTGGTTATTGGTTGAGCTTAGTACTTGTTGGTGCAAGGATGTGTTTAGGAAATCTACCTTGAACATGTGTCGTTGTTATATTGGAAAGAAATTAAAAATCAATAAATTTATGCATAGCAGTCATGTGGGCGTGATTCTCATGCAGCTTTCCTTGCCTCTTAGCATAGTTTCAGTCTTCTTATGCATTTGTTCTCTCTTTTTTATTATGGTTTTCTTCTTTATTTATTTGTCTTTGACAATTTTTGATGTGCAGGCACATTATATGCTAGGACTAGCATTAGTACAAAATAAAGTATATCCACAAGGAATCAAGCATTTGGAGAAGGTAACTGATGATTTTTATATTGTACCTTCTCTTCCTTCACTTTTGGTTAGAAAACAATTAATTTTATCATAGATATGAAATTGAGTAGAAAGTTGGATCGAAGTTTCATTTGAACCCAAAAATAACCCTTGCATTCATAGCCTCACTAGTATCACTGTGAAGGAGGGGGCAATTGGCCAAGTTGGAGTAGGTTTATATTTTCAACTTCTCTCCATTTTTTCAGAACTCACAATTGTGATTTGCCAAATAGTTATGGCGGTTTTTCTTGTTGATTCCAACTCTATCTAGCACATGGTTAGTATGGGTCAATCCCTTTTGATTGGGTCTGAGTAGGGGGTCATGGCATATCTTGGAATCTTTGGAAAGGCATTGCAGAAAATTTGTGTTACTTTTTAGTTTTGTTTCCCATTCAATGGGAGACGGGAGGGATATTTTCTTTTGAGAGGATAATTGGATTGGGATTGCTCTTTTTGCTCGTTTGTTTCATTTATTCCTTATGAAATGTGGATGTGTTGGCTTCTTTTGGGCATCATCCTTCTCTATCTTTGAATTTCCAGCGCTCTGTCTGACAGGAAGATGTTGGATATGGTTGCCCTTATTGTTCTTGTGGGGGATTTTTGTTGGGGTCATAAAGATGTTGTTGGGTTCCTAAGGGATGAGACACAAGGGACCGGGGTGATAGTAAAATTAAATTTTTCTGCAGTAAAAGCGTAGCAACAAGACTCTTCTATTGAAACAACTGCAGTTCTCAGATCTACACTAAAGGATTTTTGCATCAGTTTGCCAAGATAAAAACCAGGAACTGCCAAGTTTACTACTACATAGAAGCGCAAGGATCATGCAATATGGCTTAGTTAGTGCTTGGGCAGTGAACGACAGCGCACCAGGGTGCTGGGAGCCGGCACGCCCATGTGATATATCCTCAGTCTAATCATCCACAAATGGATTGTGAGGGTGTTTAACAACCAGAGTTATAGATGAATATTTAAGATGTTGTGGTGTAATTACCAAACTGTATTTTTACTATTATGGGCTTGCTTAGTGAAGTTAGTGGTCATTATAAATGTCTGGTAAATGATAAAGTGCTTAGAGGTTATAATCTATGGGTTCAATCCGTGATGACCATACTAAGAAATTAATTTTCAACGAGTTAAACCAATTATTTCATGAGAATAATCAAGGTGTGTGTAAACTGACTCTCACACTCATAGATATCAATTTTTTTTATCGAACTTCATTATTAGGAAATATAGGCAATCAAATTTGGAGTTTGACTGGTTCTGCTAATCACTCTACAGAATTGGCTTATTTATGATGAGAAAGGTTTCAGTTAAATTAATTTTTTTCAAGAATGTAGCAAGGTATCGGTTTTGAATGAAACTATATTTTTAGACATTCGGTTTGTATATATGTTGTATTCTGCCACTGTGACTGGGCATGTACAAATAAAATTTATTCAATCTCTTTTTATAGGCTTTGGATCTTGGTCGGGGTGCAAATCCAAAGAGTTATATGGTAGAGGAGATATGGCAGGAGCTTGCAAAAGCAAAATACAGAGAGTGGGAAAAAGCATCCACCGAACGTTCATGGGAATTGCAGAGCTTGAAGTAAGTTATTTTTCTGAGTTCTGGCATTTGGTTTCTTTCTCGAGATATCTTACGGTCGAACTAGAGTTGGATGCATGTACACCTAACCCTGAAATAAGTTTTTTTTAATCTGAAAATGTCAATAAAAATGATGCTAAACCATCATTTCCAAAGAGGTTGTAGGTAGAATCCATAGTGGCCACTTACTTCCGGAAAGATTTCCTTGGCATCCAAATGTGGTAGGGTCGAACATTATGTCTTGTGTGAATATTGGGGGTGCACGTGTGACTCAAACACTGACGGATATTGAAAGAAACACAAAAAAATAATAATGACGAATCAAAGTCACCCAGTGTTGGCGTAATGGTCAATAAGGACCGTGTAAATAATGAAAGGATCAAAGGGAAAGAGTTCTAAGTTATGGTGATCACTACCTAAAATTCATTATCTTATGGCTAATCATAGTAATCATTGTAATATGGTTAAGTAGTTGTTCCATAAGAATAGTTGAGGTGCACACAAGTTTACGACTATTAAAAGTAAAAGGAAAAAGATATCTATTAGAGTAAAACTTTCCTACGCATGGATGTGTAGGGGAGTCCTACAATTTGATGATGTGGCACCATTGGTGCCACAAAAATTCCTTAATATATATATATATATTTTTTATTTATGAGATGATTTTTTGTGGCACCAAATGGTGCCACATACTCAAAATGTAGGGCTCCCTACACATCCATGTGTAGGGGAGTTTTACTCTATCTATTATTTTCCATTTTGTTTCTTTGTTTTTGTTGTGATGAATGTACTGTTTCTTAGAGAGGCCTGTGAAGCTGCCCTGGAACAAAAATATTTTCTTGAGCAGTCTGATCCAGAAGGGTTCGTAGATGAAACCGAAATTGCTCATCAGCTACAATTGAAGTCTCTAAGAAGCCTGTTTGAAGAAGTCGCATTGACGGATGCGCCGTCTGAGGTTAGCGTAATGACTCCACTTTTTTCTTTGTTAGTTTTTAGGCATAGCATCTGCTATTAAATCCTGTGGAAATGTACTAATTTGATATTTTCAGGTGCCTGATTATCTTTGTTGTAAAATTACACTCGATATTCTCCGTGATCCTGTCATTACTCCGAGTGGGGTTACATATGAGAGAGCAGTGATTCTCGATCACTTTCAAAAGGTTACATTATACCATGCACTGGCATTTTCACGCATCGCTATCCTCCATGTCTTCATAGTCGTAGCTACAATTCTAACCACTTTCTTCCTTATCTAGGTGGGTAACTTCGATCCCATAACGCGGGAACGGCTTAACGAGTCGCAGCTAGTACCAAACTTGGCCATAAAGGAAGCAGTACAAGCGTTTCTAGATAAGCATGGATGGGCTTATAATACAGAGTAAGAAAGTTTGTATTGGATTGAAGGGAACTCAATCAAGATGGTTATGCCTGTCATGAACTAATGGAAAATTTACGTCGATCCCGTGTCGTTGATGTCGTTATTATGTCTTCTATTTCCGATTAAGCTTTGGCGCAAGATAAATGTTTTCCACGACGTTCTGAGCACGTACTGTCAATTAAGTATATGAGGTCCTGTCTTTAGATTTGATCTTGTGCAGATTGTCAATATGACCTGTTAGTGTTCTTGTACAGTTGCTTATAAAAGTTTAAACTATAATGGTTTAGCTGTT

mRNA sequence

ATTTGTTCAATTTTTTAAACCTGGAAACGTAAATTTTCGGGTGGAAATTTGTTGAACCCTCAATCACTTCGTTCTGTTCTTCGTTTCCCACTTTTCTCTGAATCAAAATTTCTTTCTTCTTCTTCTTCTTCTTCAATTGGGAAATCATTCTCTGAAATCCCATTTATGGAAATTTCTCTAACTTCGTAATTTCTTGACCTAAACAATTCCATTGATGGGGGAGGGCTTGTCTGACCCCGCAAAGCAGGCCGAGCTGCTCAGAAAAGATGGAAACCTCTATTTCCAGAAGGGCCGAATTGGAGCCGCCATTGATGCCTACACCGAGGCAATTACGTTGTGCCCGAATGTTCCTGTGTATTTGACGAACCGTGCTTTGTGTCATCGCAAGAGGAATGATTGGAAGAAGGTGGAGGAGGACTGTCGGAGAGCTATTCAGCTCGATAGCAACTCTGTCAAGGCACATTATATGCTAGGACTAGCATTAGTACAAAATAAAGTATATCCACAAGGAATCAAGCATTTGGAGAAGGCTTTGGATCTTGGTCGGGGTGCAAATCCAAAGAGTTATATGGTAGAGGAGATATGGCAGGAGCTTGCAAAAGCAAAATACAGAGAGTGGGAAAAAGCATCCACCGAACGTTCATGGGAATTGCAGAGCTTGAAAGAGGCCTGTGAAGCTGCCCTGGAACAAAAATATTTTCTTGAGCAGTCTGATCCAGAAGGGTTCGTAGATGAAACCGAAATTGCTCATCAGCTACAATTGAAGTCTCTAAGAAGCCTGTTTGAAGAAGTCGCATTGACGGATGCGCCGTCTGAGGTGCCTGATTATCTTTGTTGTAAAATTACACTCGATATTCTCCGTGATCCTGTCATTACTCCGAGTGGGGTTACATATGAGAGAGCAGTGATTCTCGATCACTTTCAAAAGGTGGGTAACTTCGATCCCATAACGCGGGAACGGCTTAACGAGTCGCAGCTAGTACCAAACTTGGCCATAAAGGAAGCAGTACAAGCGTTTCTAGATAAGCATGGATGGGCTTATAATACAGAGTAAGAAAGTTTGTATTGGATTGAAGGGAACTCAATCAAGATGGTTATGCCTGTCATGAACTAATGGAAAATTTACGTCGATCCCGTGTCGTTGATGTCGTTATTATGTCTTCTATTTCCGATTAAGCTTTGGCGCAAGATAAATGTTTTCCACGACGTTCTGAGCACGTACTGTCAATTAAGTATATGAGGTCCTGTCTTTAGATTTGATCTTGTGCAGATTGTCAATATGACCTGTTAGTGTTCTTGTACAGTTGCTTATAAAAGTTTAAACTATAATGGTTTAGCTGTT

Coding sequence (CDS)

ATGGGGGAGGGCTTGTCTGACCCCGCAAAGCAGGCCGAGCTGCTCAGAAAAGATGGAAACCTCTATTTCCAGAAGGGCCGAATTGGAGCCGCCATTGATGCCTACACCGAGGCAATTACGTTGTGCCCGAATGTTCCTGTGTATTTGACGAACCGTGCTTTGTGTCATCGCAAGAGGAATGATTGGAAGAAGGTGGAGGAGGACTGTCGGAGAGCTATTCAGCTCGATAGCAACTCTGTCAAGGCACATTATATGCTAGGACTAGCATTAGTACAAAATAAAGTATATCCACAAGGAATCAAGCATTTGGAGAAGGCTTTGGATCTTGGTCGGGGTGCAAATCCAAAGAGTTATATGGTAGAGGAGATATGGCAGGAGCTTGCAAAAGCAAAATACAGAGAGTGGGAAAAAGCATCCACCGAACGTTCATGGGAATTGCAGAGCTTGAAAGAGGCCTGTGAAGCTGCCCTGGAACAAAAATATTTTCTTGAGCAGTCTGATCCAGAAGGGTTCGTAGATGAAACCGAAATTGCTCATCAGCTACAATTGAAGTCTCTAAGAAGCCTGTTTGAAGAAGTCGCATTGACGGATGCGCCGTCTGAGGTGCCTGATTATCTTTGTTGTAAAATTACACTCGATATTCTCCGTGATCCTGTCATTACTCCGAGTGGGGTTACATATGAGAGAGCAGTGATTCTCGATCACTTTCAAAAGGTGGGTAACTTCGATCCCATAACGCGGGAACGGCTTAACGAGTCGCAGCTAGTACCAAACTTGGCCATAAAGGAAGCAGTACAAGCGTTTCTAGATAAGCATGGATGGGCTTATAATACAGAGTAA

Protein sequence

MGEGLSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRKRNDWKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSYMVEEIWQELAKAKYREWEKASTERSWELQSLKEACEAALEQKYFLEQSDPEGFVDETEIAHQLQLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQKVGNFDPITRERLNESQLVPNLAIKEAVQAFLDKHGWAYNTE
Homology
BLAST of Sed0023145.1 vs. NCBI nr
Match: XP_038886937.1 (E3 ubiquitin-protein ligase CHIP isoform X1 [Benincasa hispida])

HSP 1 Score: 511.1 bits (1315), Expect = 5.8e-141
Identity = 250/277 (90.25%), Postives = 264/277 (95.31%), Query Frame = 0

Query: 2   GEGLSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRKRND 61
           G  LS  AKQAELLRKDGN YFQKGRIGAAI+AYTEAITLCPNVPVYLTNRALCHRKRND
Sbjct: 4   GVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVPVYLTNRALCHRKRND 63

Query: 62  WKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSYMVE 121
           WKKVEEDCRRAIQLDS+SVKAHYMLGLAL+QNK YP+GIKHLEKALDLGRGANPKSY+VE
Sbjct: 64  WKKVEEDCRRAIQLDSSSVKAHYMLGLALIQNKEYPEGIKHLEKALDLGRGANPKSYIVE 123

Query: 122 EIWQELAKAKYREWEKASTERSWELQSLKEACEAALEQKYFLEQSDPEGFVDETEIAHQL 181
           EIWQELAKAKYREWE+ASTERSWELQ+LKEACEAALEQKYFL+QS+ EGFVDE EIAH+ 
Sbjct: 124 EIWQELAKAKYREWEQASTERSWELQTLKEACEAALEQKYFLDQSELEGFVDEAEIAHRN 183

Query: 182 QLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQKVGN 241
           QLKSLRS+FE+VA  DAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHF KVGN
Sbjct: 184 QLKSLRSVFEKVAEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFHKVGN 243

Query: 242 FDPITRERLNESQLVPNLAIKEAVQAFLDKHGWAYNT 279
           FDPITRE L+ESQLVPNLAIKEAVQ+FLDKHGWAYNT
Sbjct: 244 FDPITRELLDESQLVPNLAIKEAVQSFLDKHGWAYNT 280

BLAST of Sed0023145.1 vs. NCBI nr
Match: XP_022931210.1 (E3 ubiquitin-protein ligase CHIP-like [Cucurbita moschata] >XP_022995799.1 E3 ubiquitin-protein ligase CHIP-like [Cucurbita maxima])

HSP 1 Score: 506.1 bits (1302), Expect = 1.9e-139
Identity = 248/282 (87.94%), Postives = 264/282 (93.62%), Query Frame = 0

Query: 1   MGEG---LSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHR 60
           MG G   LS  AKQAELLRKDGN YFQKGRIGAAI+AYTEAITLCPNV VYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRNDWKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKS 120
           KRNDWKKVEEDCRRAIQ+DSNSVK HYMLGLAL+QN VYP+GIKHLEKALDLGRGANPKS
Sbjct: 61  KRNDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDLGRGANPKS 120

Query: 121 YMVEEIWQELAKAKYREWEKASTERSWELQSLKEACEAALEQKYFLEQSDPEGFVDETEI 180
           YMVEEIWQELAKAKYREWEKAST+RSWELQ+LKEACEAALEQKYFL+ S+ EGFVDE EI
Sbjct: 121 YMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELEGFVDEAEI 180

Query: 181 AHQLQLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQ 240
           AH+ QLKSLRS+FE+V   DAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHF+
Sbjct: 181 AHRKQLKSLRSVFEKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR 240

Query: 241 KVGNFDPITRERLNESQLVPNLAIKEAVQAFLDKHGWAYNTE 280
           KVGNFDP+TRE L+ESQLVPNLAIKEAV+AFLDKHGWAYNT+
Sbjct: 241 KVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 282

BLAST of Sed0023145.1 vs. NCBI nr
Match: XP_004144393.1 (E3 ubiquitin-protein ligase CHIP isoform X1 [Cucumis sativus] >KGN58379.1 hypothetical protein Csa_002406 [Cucumis sativus])

HSP 1 Score: 505.0 bits (1299), Expect = 4.2e-139
Identity = 246/281 (87.54%), Postives = 264/281 (93.95%), Query Frame = 0

Query: 1   MGEG--LSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRK 60
           MG G  LS PAKQAE+LRKDGN YFQKGRIGAAI+AYTEAITLCPNVPVYLTNRALCHRK
Sbjct: 1   MGPGVPLSGPAKQAEILRKDGNHYFQKGRIGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60

Query: 61  RNDWKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSY 120
           RNDW KVEEDCRRAIQLDS+SVKAHYMLGLAL+QNK YP+GIKHLEKALDLGRG NPKSY
Sbjct: 61  RNDWNKVEEDCRRAIQLDSSSVKAHYMLGLALLQNKEYPEGIKHLEKALDLGRGENPKSY 120

Query: 121 MVEEIWQELAKAKYREWEKASTERSWELQSLKEACEAALEQKYFLEQSDPEGFVDETEIA 180
           +VEEIWQELAKAKYREWE+ASTERSWELQ+LKEACEAALEQKYFL+QS+ EGFVDE +IA
Sbjct: 121 IVEEIWQELAKAKYREWEQASTERSWELQTLKEACEAALEQKYFLDQSELEGFVDEADIA 180

Query: 181 HQLQLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQK 240
           H+ QLKSLRS+FE+V   D+PSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHF K
Sbjct: 181 HRKQLKSLRSVFEKVTEADSPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFNK 240

Query: 241 VGNFDPITRERLNESQLVPNLAIKEAVQAFLDKHGWAYNTE 280
           VGNFDPITRE LNESQL+PNLAIKEAVQ+FLDKHGWAYN +
Sbjct: 241 VGNFDPITRELLNESQLIPNLAIKEAVQSFLDKHGWAYNMD 281

BLAST of Sed0023145.1 vs. NCBI nr
Match: KAG6606301.1 (E3 ubiquitin-protein ligase CHIP, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 504.6 bits (1298), Expect = 5.5e-139
Identity = 247/282 (87.59%), Postives = 264/282 (93.62%), Query Frame = 0

Query: 1   MGEG---LSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHR 60
           MG G   LS  AKQAELLRKDGN YFQKGRIGAAI+AYTEAITLCPNV VYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRNDWKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKS 120
           KRNDWKKVEEDCRRAIQ+DSNSVK HYMLGLAL+QN VYP+GIKHLEKALDLGRGANPKS
Sbjct: 61  KRNDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDLGRGANPKS 120

Query: 121 YMVEEIWQELAKAKYREWEKASTERSWELQSLKEACEAALEQKYFLEQSDPEGFVDETEI 180
           YMVEEIWQELAKAKYREWEKAST+RSWELQ+LKEACEAALEQKYFL+ S+ EGFVDE EI
Sbjct: 121 YMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELEGFVDEAEI 180

Query: 181 AHQLQLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQ 240
           AH+ QLKSLRS+F++V   DAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHF+
Sbjct: 181 AHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR 240

Query: 241 KVGNFDPITRERLNESQLVPNLAIKEAVQAFLDKHGWAYNTE 280
           KVGNFDP+TRE L+ESQLVPNLAIKEAV+AFLDKHGWAYNT+
Sbjct: 241 KVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 282

BLAST of Sed0023145.1 vs. NCBI nr
Match: XP_023553676.1 (E3 ubiquitin-protein ligase CHIP-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 502.7 bits (1293), Expect = 2.1e-138
Identity = 245/280 (87.50%), Postives = 264/280 (94.29%), Query Frame = 0

Query: 1   MGEG--LSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRK 60
           MG G  LS  A+QAE+LRKDGN YFQKGRIGAAI+AYTEAITLCPNVPVYLTNRALCHRK
Sbjct: 1   MGPGVFLSGAARQAEILRKDGNHYFQKGRIGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60

Query: 61  RNDWKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSY 120
           RNDWKKVEEDCRRAIQLD+NSVKAHYM+GLAL+QNK YP+GIKHLEKALDLGRGAN KSY
Sbjct: 61  RNDWKKVEEDCRRAIQLDNNSVKAHYMVGLALIQNKDYPEGIKHLEKALDLGRGANQKSY 120

Query: 121 MVEEIWQELAKAKYREWEKASTERSWELQSLKEACEAALEQKYFLEQSDPEGFVDETEIA 180
           MVEEIWQELA AKYREWE+AST+RSWELQ+LKEACEAALEQKYFL+QS+PEGFVD+ EIA
Sbjct: 121 MVEEIWQELANAKYREWEQASTKRSWELQTLKEACEAALEQKYFLDQSEPEGFVDQAEIA 180

Query: 181 HQLQLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQK 240
           H+ QL SLRS+FE  A+ DAPSEVPD+LCCKITLDILRDPVITPSGVTYERAVILDHF+K
Sbjct: 181 HREQLNSLRSVFENYAVADAPSEVPDHLCCKITLDILRDPVITPSGVTYERAVILDHFRK 240

Query: 241 VGNFDPITRERLNESQLVPNLAIKEAVQAFLDKHGWAYNT 279
           VGNFDPITRE L+ESQLVPNLAIKEAVQAFLDKHGWAYNT
Sbjct: 241 VGNFDPITRELLDESQLVPNLAIKEAVQAFLDKHGWAYNT 280

BLAST of Sed0023145.1 vs. ExPASy Swiss-Prot
Match: Q9SRS9 (E3 ubiquitin-protein ligase CHIP OS=Arabidopsis thaliana OX=3702 GN=CHIP PE=1 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 5.8e-99
Identity = 177/265 (66.79%), Postives = 207/265 (78.11%), Query Frame = 0

Query: 12  AELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRKRNDWKKVEEDCRR 71
           AE L++DGN  F+K R GAAIDAYTEAI L PNVP Y TNRALCH KR DW KVEEDCR+
Sbjct: 10  AERLKEDGNNCFKKERFGAAIDAYTEAIALSPNVPAYWTNRALCHMKRKDWTKVEEDCRK 69

Query: 72  AIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSYMVEEIWQELAKAK 131
           AIQL  NSVKAHYMLGLAL+Q K +  G+K L++ALDLGR +NP  YMVEEIW+EL+KAK
Sbjct: 70  AIQLVHNSVKAHYMLGLALLQKKEFTNGVKELQRALDLGRCSNPTGYMVEEIWEELSKAK 129

Query: 132 YREWEKASTERSWELQSLKEACEAALEQKYFLEQSDPEGFVDETEIAHQLQLKSLRSLFE 191
           Y EWE  S  RSWEL SLKE CEAAL Q+  L+ S  E   DE   AH  +LK+L  +F+
Sbjct: 130 YMEWELVSAMRSWELNSLKETCEAALNQQRALDMSRTEESSDEAYTAHTERLKALERVFK 189

Query: 192 EVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQKVGNFDPITRERLN 251
           + A  D P+EVPDYLCC ITL+I RDPVI+PSGVTYERA IL+H +KVG FDPITRE+++
Sbjct: 190 KAAEEDKPTEVPDYLCCNITLEIFRDPVISPSGVTYERAAILEHLKKVGKFDPITREKID 249

Query: 252 ESQLVPNLAIKEAVQAFLDKHGWAY 277
            + LVPNLAIKEAV A+L+KH WAY
Sbjct: 250 PANLVPNLAIKEAVAAYLEKHVWAY 274

BLAST of Sed0023145.1 vs. ExPASy Swiss-Prot
Match: Q9WUD1 (E3 ubiquitin-protein ligase CHIP OS=Mus musculus OX=10090 GN=Stub1 PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 6.9e-44
Identity = 104/286 (36.36%), Postives = 156/286 (54.55%), Query Frame = 0

Query: 2   GEGLSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRKRND 61
           G G  D +  A+ L++ GN  F   +   A   Y  AIT  P V VY TNRALC+ K   
Sbjct: 17  GGGSPDKSPSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALCYLKMQQ 76

Query: 62  WKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSYMVE 121
            ++   DCRRA++LD  SVKAH+ LG   ++ + Y + I +L++A  L +    +    +
Sbjct: 77  PEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAK--EQRLNFGD 136

Query: 122 EIWQELAKAKYREW---EKASTERSWELQSLKEACEAALEQKYFLE-QSDPEGFVDE--- 181
           +I   L  AK + W   E+    +  EL S      AA  ++   E Q + EG  D+   
Sbjct: 137 DIPSALRIAKKKRWNSIEERRIHQESELHSYLTRLIAAERERELEECQRNHEGHEDDGHI 196

Query: 182 ------TEIAHQLQLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYE 241
                  E  H   +  +  LF +V       ++PDYLC KI+ +++R+P ITPSG+TY+
Sbjct: 197 RAQQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYD 256

Query: 242 RAVILDHFQKVGNFDPITRERLNESQLVPNLAIKEAVQAFLDKHGW 275
           R  I +H Q+VG+FDP+TR  L + QL+PNLA+KE + AF+ ++GW
Sbjct: 257 RKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGW 300

BLAST of Sed0023145.1 vs. ExPASy Swiss-Prot
Match: Q9UNE7 (E3 ubiquitin-protein ligase CHIP OS=Homo sapiens OX=9606 GN=STUB1 PE=1 SV=2)

HSP 1 Score: 178.7 bits (452), Expect = 9.0e-44
Identity = 103/286 (36.01%), Postives = 157/286 (54.90%), Query Frame = 0

Query: 2   GEGLSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRKRND 61
           G G  + +  A+ L++ GN  F   +   A   Y  AIT  P V VY TNRALC+ K   
Sbjct: 16  GGGSPEKSPSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALCYLKMQQ 75

Query: 62  WKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSYMVE 121
            ++   DCRRA++LD  SVKAH+ LG   ++ + Y + I +L++A  L +    +    +
Sbjct: 76  HEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAK--EQRLNFGD 135

Query: 122 EIWQELAKAKYREW---EKASTERSWELQSLKEACEAALEQKYFLE-QSDPEGFVDET-- 181
           +I   L  AK + W   E+    +  EL S      AA  ++   E Q + EG  D++  
Sbjct: 136 DIPSALRIAKKKRWNSIEERRIHQESELHSYLSRLIAAERERELEECQRNHEGDEDDSHV 195

Query: 182 -------EIAHQLQLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYE 241
                  E  H   +  +  LF +V       ++PDYLC KI+ +++R+P ITPSG+TY+
Sbjct: 196 RAQQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYD 255

Query: 242 RAVILDHFQKVGNFDPITRERLNESQLVPNLAIKEAVQAFLDKHGW 275
           R  I +H Q+VG+FDP+TR  L + QL+PNLA+KE + AF+ ++GW
Sbjct: 256 RKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGW 299

BLAST of Sed0023145.1 vs. ExPASy Swiss-Prot
Match: Q5ZHY5 (E3 ubiquitin-protein ligase CHIP OS=Gallus gallus OX=9031 GN=STUB1 PE=2 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.9e-42
Identity = 102/287 (35.54%), Postives = 156/287 (54.36%), Query Frame = 0

Query: 2   GEGLSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRKRND 61
           G G  + +  A+  ++ GN  F   +   A  AY  AI   P V VY TNRALC+ K   
Sbjct: 26  GGGSPEKSHSAQEHKEQGNRLFGGRKYPEAAAAYGRAINRNPLVAVYYTNRALCYLKMQQ 85

Query: 62  WKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSYMVE 121
             K   DC+RA++LD  SVKAH+ LG   ++ + Y + I +L++A +L +    +    +
Sbjct: 86  HDKALADCKRALELDGQSVKAHFFLGQCQMEMENYDEAIANLQRAYNLAK--EQRLNFGD 145

Query: 122 EIWQELAKAKYREW---EKASTERSWELQSLKEACEAALEQKYFLE--QSDPEGFVDET- 181
           +I   L  AK + W   E+    +  EL S       A +++   E  ++  E  VDE+ 
Sbjct: 146 DIPSALRIAKKKRWNSIEEKRINQENELHSYLTRLIMAEKERELAECRKAQQEENVDESR 205

Query: 182 --------EIAHQLQLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTY 241
                   E  H   L  +  LF +V       ++PDYLC KI+ +++R+P ITPSG+TY
Sbjct: 206 GRVQLAGIEAKHDKYLADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITY 265

Query: 242 ERAVILDHFQKVGNFDPITRERLNESQLVPNLAIKEAVQAFLDKHGW 275
           +R  I +H Q+VG+FDP+TR  L + QL+PNLA+KE + AF+ ++GW
Sbjct: 266 DRKDIEEHLQRVGHFDPVTRSPLTQDQLIPNLAMKEVIDAFISENGW 310

BLAST of Sed0023145.1 vs. ExPASy Swiss-Prot
Match: Q8VD33 (Small glutamine-rich tetratricopeptide repeat-containing protein beta OS=Mus musculus OX=10090 GN=Sgtb PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.1e-12
Identity = 37/109 (33.94%), Postives = 60/109 (55.05%), Query Frame = 0

Query: 1   MGEGLSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRKRN 60
           +   + +   +A+ L+ +GN + ++    AA+D YT+AI L PN  VY  NRA    K +
Sbjct: 74  LSNSVPEDVGKADQLKDEGNNHMKEENYAAAVDCYTQAIELDPNNAVYYCNRAAAQSKLS 133

Query: 61  DWKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDL 110
            +    +DC +AI +DS   KA+  +GLAL     + + +   +KALDL
Sbjct: 134 HYTDAIKDCEKAIAIDSKYSKAYGRMGLALTAMNKFEEAVTSYQKALDL 182

BLAST of Sed0023145.1 vs. ExPASy TrEMBL
Match: A0A6J1K6X7 (E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita maxima OX=3661 GN=LOC111491225 PE=4 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 9.1e-140
Identity = 248/282 (87.94%), Postives = 264/282 (93.62%), Query Frame = 0

Query: 1   MGEG---LSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHR 60
           MG G   LS  AKQAELLRKDGN YFQKGRIGAAI+AYTEAITLCPNV VYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRNDWKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKS 120
           KRNDWKKVEEDCRRAIQ+DSNSVK HYMLGLAL+QN VYP+GIKHLEKALDLGRGANPKS
Sbjct: 61  KRNDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDLGRGANPKS 120

Query: 121 YMVEEIWQELAKAKYREWEKASTERSWELQSLKEACEAALEQKYFLEQSDPEGFVDETEI 180
           YMVEEIWQELAKAKYREWEKAST+RSWELQ+LKEACEAALEQKYFL+ S+ EGFVDE EI
Sbjct: 121 YMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELEGFVDEAEI 180

Query: 181 AHQLQLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQ 240
           AH+ QLKSLRS+FE+V   DAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHF+
Sbjct: 181 AHRKQLKSLRSVFEKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR 240

Query: 241 KVGNFDPITRERLNESQLVPNLAIKEAVQAFLDKHGWAYNTE 280
           KVGNFDP+TRE L+ESQLVPNLAIKEAV+AFLDKHGWAYNT+
Sbjct: 241 KVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 282

BLAST of Sed0023145.1 vs. ExPASy TrEMBL
Match: A0A6J1EYU4 (E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111437457 PE=4 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 9.1e-140
Identity = 248/282 (87.94%), Postives = 264/282 (93.62%), Query Frame = 0

Query: 1   MGEG---LSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHR 60
           MG G   LS  AKQAELLRKDGN YFQKGRIGAAI+AYTEAITLCPNV VYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRNDWKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKS 120
           KRNDWKKVEEDCRRAIQ+DSNSVK HYMLGLAL+QN VYP+GIKHLEKALDLGRGANPKS
Sbjct: 61  KRNDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDLGRGANPKS 120

Query: 121 YMVEEIWQELAKAKYREWEKASTERSWELQSLKEACEAALEQKYFLEQSDPEGFVDETEI 180
           YMVEEIWQELAKAKYREWEKAST+RSWELQ+LKEACEAALEQKYFL+ S+ EGFVDE EI
Sbjct: 121 YMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELEGFVDEAEI 180

Query: 181 AHQLQLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQ 240
           AH+ QLKSLRS+FE+V   DAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHF+
Sbjct: 181 AHRKQLKSLRSVFEKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR 240

Query: 241 KVGNFDPITRERLNESQLVPNLAIKEAVQAFLDKHGWAYNTE 280
           KVGNFDP+TRE L+ESQLVPNLAIKEAV+AFLDKHGWAYNT+
Sbjct: 241 KVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 282

BLAST of Sed0023145.1 vs. ExPASy TrEMBL
Match: A0A0A0LBQ5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G634310 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.0e-139
Identity = 246/281 (87.54%), Postives = 264/281 (93.95%), Query Frame = 0

Query: 1   MGEG--LSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRK 60
           MG G  LS PAKQAE+LRKDGN YFQKGRIGAAI+AYTEAITLCPNVPVYLTNRALCHRK
Sbjct: 1   MGPGVPLSGPAKQAEILRKDGNHYFQKGRIGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60

Query: 61  RNDWKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSY 120
           RNDW KVEEDCRRAIQLDS+SVKAHYMLGLAL+QNK YP+GIKHLEKALDLGRG NPKSY
Sbjct: 61  RNDWNKVEEDCRRAIQLDSSSVKAHYMLGLALLQNKEYPEGIKHLEKALDLGRGENPKSY 120

Query: 121 MVEEIWQELAKAKYREWEKASTERSWELQSLKEACEAALEQKYFLEQSDPEGFVDETEIA 180
           +VEEIWQELAKAKYREWE+ASTERSWELQ+LKEACEAALEQKYFL+QS+ EGFVDE +IA
Sbjct: 121 IVEEIWQELAKAKYREWEQASTERSWELQTLKEACEAALEQKYFLDQSELEGFVDEADIA 180

Query: 181 HQLQLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQK 240
           H+ QLKSLRS+FE+V   D+PSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHF K
Sbjct: 181 HRKQLKSLRSVFEKVTEADSPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFNK 240

Query: 241 VGNFDPITRERLNESQLVPNLAIKEAVQAFLDKHGWAYNTE 280
           VGNFDPITRE LNESQL+PNLAIKEAVQ+FLDKHGWAYN +
Sbjct: 241 VGNFDPITRELLNESQLIPNLAIKEAVQSFLDKHGWAYNMD 281

BLAST of Sed0023145.1 vs. ExPASy TrEMBL
Match: A0A6J1HID6 (E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111463871 PE=4 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 5.0e-138
Identity = 244/280 (87.14%), Postives = 263/280 (93.93%), Query Frame = 0

Query: 1   MGEG--LSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRK 60
           MG G  LS  A+QAE+LRKDGN YFQKGRIGAAI+AYTEAITLCPNVPVYLTNRALCHRK
Sbjct: 1   MGPGVFLSGAARQAEILRKDGNHYFQKGRIGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60

Query: 61  RNDWKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSY 120
           RNDWKKVEEDCRRAIQLD+NSVKAHYM+GLAL+QNK YP+GIKHLEKALDLGRGAN KSY
Sbjct: 61  RNDWKKVEEDCRRAIQLDNNSVKAHYMVGLALIQNKDYPEGIKHLEKALDLGRGANQKSY 120

Query: 121 MVEEIWQELAKAKYREWEKASTERSWELQSLKEACEAALEQKYFLEQSDPEGFVDETEIA 180
           MVEEIWQELA AKYREWE+AST+RSWELQ+LKEACEAALEQKYFL+QS+PEGFVD+ E A
Sbjct: 121 MVEEIWQELANAKYREWEQASTKRSWELQTLKEACEAALEQKYFLDQSEPEGFVDQAESA 180

Query: 181 HQLQLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQK 240
           H+ QL SLRS+FE  A+ DAPSEVPD+LCCKITLDILRDPVITPSGVTYERAVILDHF+K
Sbjct: 181 HREQLNSLRSVFENYAVADAPSEVPDHLCCKITLDILRDPVITPSGVTYERAVILDHFRK 240

Query: 241 VGNFDPITRERLNESQLVPNLAIKEAVQAFLDKHGWAYNT 279
           VGNFDPITRE L+ESQLVPNLAIKEAVQAFLDKHGWAYNT
Sbjct: 241 VGNFDPITRELLDESQLVPNLAIKEAVQAFLDKHGWAYNT 280

BLAST of Sed0023145.1 vs. ExPASy TrEMBL
Match: A0A6J1HQG3 (E3 ubiquitin-protein ligase CHIP-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466868 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.5e-137
Identity = 243/280 (86.79%), Postives = 263/280 (93.93%), Query Frame = 0

Query: 1   MGEG--LSDPAKQAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRK 60
           MG G  LS  A+QAE+LRKDGN YFQKGRIGAAI+AYTEAITLCPNVPVYLTNRALCHRK
Sbjct: 1   MGPGVFLSGAARQAEILRKDGNHYFQKGRIGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60

Query: 61  RNDWKKVEEDCRRAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSY 120
           RNDWKKVEEDCRRAIQLD+NSVKAHYM+GLAL+QNK YP+GIKHLEKALDLGRGAN KSY
Sbjct: 61  RNDWKKVEEDCRRAIQLDNNSVKAHYMVGLALIQNKDYPEGIKHLEKALDLGRGANQKSY 120

Query: 121 MVEEIWQELAKAKYREWEKASTERSWELQSLKEACEAALEQKYFLEQSDPEGFVDETEIA 180
           MVEEIWQELA AKYREWE+AST+RSWELQ+LKEACEAALEQKYFL+QS+PEGFVD+ E A
Sbjct: 121 MVEEIWQELANAKYREWEQASTKRSWELQTLKEACEAALEQKYFLDQSEPEGFVDQAESA 180

Query: 181 HQLQLKSLRSLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQK 240
           H+ QL SLRS+FE  A+ DAPSEVPD+LCCKITLDILRDPVITPSGVTYERAVILDHF+K
Sbjct: 181 HREQLNSLRSVFENYAVADAPSEVPDHLCCKITLDILRDPVITPSGVTYERAVILDHFRK 240

Query: 241 VGNFDPITRERLNESQLVPNLAIKEAVQAFLDKHGWAYNT 279
           VGNFDPITRE L+ESQLVPNLAIKEAVQAFLDKHGWAYN+
Sbjct: 241 VGNFDPITRELLDESQLVPNLAIKEAVQAFLDKHGWAYNS 280

BLAST of Sed0023145.1 vs. TAIR 10
Match: AT3G07370.1 (carboxyl terminus of HSC70-interacting protein )

HSP 1 Score: 362.1 bits (928), Expect = 4.1e-100
Identity = 177/265 (66.79%), Postives = 207/265 (78.11%), Query Frame = 0

Query: 12  AELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRKRNDWKKVEEDCRR 71
           AE L++DGN  F+K R GAAIDAYTEAI L PNVP Y TNRALCH KR DW KVEEDCR+
Sbjct: 10  AERLKEDGNNCFKKERFGAAIDAYTEAIALSPNVPAYWTNRALCHMKRKDWTKVEEDCRK 69

Query: 72  AIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSYMVEEIWQELAKAK 131
           AIQL  NSVKAHYMLGLAL+Q K +  G+K L++ALDLGR +NP  YMVEEIW+EL+KAK
Sbjct: 70  AIQLVHNSVKAHYMLGLALLQKKEFTNGVKELQRALDLGRCSNPTGYMVEEIWEELSKAK 129

Query: 132 YREWEKASTERSWELQSLKEACEAALEQKYFLEQSDPEGFVDETEIAHQLQLKSLRSLFE 191
           Y EWE  S  RSWEL SLKE CEAAL Q+  L+ S  E   DE   AH  +LK+L  +F+
Sbjct: 130 YMEWELVSAMRSWELNSLKETCEAALNQQRALDMSRTEESSDEAYTAHTERLKALERVFK 189

Query: 192 EVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQKVGNFDPITRERLN 251
           + A  D P+EVPDYLCC ITL+I RDPVI+PSGVTYERA IL+H +KVG FDPITRE+++
Sbjct: 190 KAAEEDKPTEVPDYLCCNITLEIFRDPVISPSGVTYERAAILEHLKKVGKFDPITREKID 249

Query: 252 ESQLVPNLAIKEAVQAFLDKHGWAY 277
            + LVPNLAIKEAV A+L+KH WAY
Sbjct: 250 PANLVPNLAIKEAVAAYLEKHVWAY 274

BLAST of Sed0023145.1 vs. TAIR 10
Match: AT3G61390.2 (RING/U-box superfamily protein )

HSP 1 Score: 68.2 bits (165), Expect = 1.2e-11
Identity = 56/181 (30.94%), Postives = 83/181 (45.86%), Query Frame = 0

Query: 104 EKALDLGRGANPKSYMVEEIWQELAKAKYREWEKASTERSWELQSLKEACEA-------- 163
           E+A      A  K+   E ++ E  K + +E EKA  +R+ EL+ ++   E         
Sbjct: 243 EEAEKTAVDALKKAKQWENVYFEELKQR-KETEKALRKRNDELEKMRSESETQITESYTV 302

Query: 164 --ALEQKYFLEQSDPEGFVDETE-----IAHQLQLKSLRSLFEEVALTDAPSEVPDYLCC 223
              L++K  L      G  +E E     +    +LK  R   EE A T    E P Y  C
Sbjct: 303 IRKLQEKNNLSMETFRGIREEQEELKIKLREVSKLKGKRE--EEEASTSNHREPPQYFIC 362

Query: 224 KITLDILRDPVITPSGVTYERAVILDHFQKVGNFDPITRERLNESQLVPNLAIKEAVQAF 270
            IT DI+ DP +   G TYE   I   F++     P+  +RL  + LVPNLA++ A+Q +
Sbjct: 363 PITHDIMEDPHVAADGFTYEGEAISRWFERGHETSPMINKRLPHTSLVPNLALRSAIQEW 420

BLAST of Sed0023145.1 vs. TAIR 10
Match: AT2G45920.1 (U-box domain-containing protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.7e-11
Identity = 55/204 (26.96%), Postives = 93/204 (45.59%), Query Frame = 0

Query: 95  VYPQGIKHLEKALDLGRGANPKSYMVEEIWQELAKA--KYREWEKASTE----RSWELQS 154
           VY   +   EK+    + A+ +++  +E+ +E  +A  + +EWE A  E    R      
Sbjct: 192 VYQLAVFEAEKS---KKEASLEAFKHQEVVKEKNEAIKRGKEWESAYLEELKQRKETEME 251

Query: 155 LKEACEAALEQKYFLEQSDPEGF--VDETEIAHQLQLKSLR------------------- 214
           LK+  E   + +Y  E    E +  V + +  + L  K LR                   
Sbjct: 252 LKKVREKLEKMRYISENRITESYMLVQKLQDKYNLATKVLRKAKEERDLLIKGRDIAIIE 311

Query: 215 --SLFEEVALTDAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFQKVGNFDPI 270
              L +EV+ +D   E P Y  C I+L++++DP +   G TYE   I    Q      P+
Sbjct: 312 VEELRKEVSRSDEHREAPQYFICPISLEVMKDPQLAADGFTYEAEAISTWLQGGHETSPM 371

BLAST of Sed0023145.1 vs. TAIR 10
Match: AT2G42810.1 (protein phosphatase 5.2 )

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-09
Identity = 35/124 (28.23%), Postives = 59/124 (47.58%), Query Frame = 0

Query: 11  QAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRKRNDWKKVEEDCR 70
           +AE  +   N  F+  +  +AID YT+AI L  N  VY  NRA  H K  ++    +D  
Sbjct: 12  RAEEFKSQANEAFKGHKYSSAIDLYTKAIELNSNNAVYWANRAFAHTKLEEYGSAIQDAS 71

Query: 71  RAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSYMVEEIWQELAKA 130
           +AI++DS   K +Y  G A +    +   +K  ++   L       +  ++E  + + K 
Sbjct: 72  KAIEVDSRYSKGYYRRGAAYLAMGKFKDALKDFQQVKRLSPNDPDATRKLKECEKAVMKL 131

Query: 131 KYRE 135
           K+ E
Sbjct: 132 KFEE 135

BLAST of Sed0023145.1 vs. TAIR 10
Match: AT2G42810.2 (protein phosphatase 5.2 )

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-09
Identity = 35/124 (28.23%), Postives = 59/124 (47.58%), Query Frame = 0

Query: 11  QAELLRKDGNLYFQKGRIGAAIDAYTEAITLCPNVPVYLTNRALCHRKRNDWKKVEEDCR 70
           +AE  +   N  F+  +  +AID YT+AI L  N  VY  NRA  H K  ++    +D  
Sbjct: 12  RAEEFKSQANEAFKGHKYSSAIDLYTKAIELNSNNAVYWANRAFAHTKLEEYGSAIQDAS 71

Query: 71  RAIQLDSNSVKAHYMLGLALVQNKVYPQGIKHLEKALDLGRGANPKSYMVEEIWQELAKA 130
           +AI++DS   K +Y  G A +    +   +K  ++   L       +  ++E  + + K 
Sbjct: 72  KAIEVDSRYSKGYYRRGAAYLAMGKFKDALKDFQQVKRLSPNDPDATRKLKECEKAVMKL 131

Query: 131 KYRE 135
           K+ E
Sbjct: 132 KFEE 135

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886937.15.8e-14190.25E3 ubiquitin-protein ligase CHIP isoform X1 [Benincasa hispida][more]
XP_022931210.11.9e-13987.94E3 ubiquitin-protein ligase CHIP-like [Cucurbita moschata] >XP_022995799.1 E3 ub... [more]
XP_004144393.14.2e-13987.54E3 ubiquitin-protein ligase CHIP isoform X1 [Cucumis sativus] >KGN58379.1 hypoth... [more]
KAG6606301.15.5e-13987.59E3 ubiquitin-protein ligase CHIP, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023553676.12.1e-13887.50E3 ubiquitin-protein ligase CHIP-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SRS95.8e-9966.79E3 ubiquitin-protein ligase CHIP OS=Arabidopsis thaliana OX=3702 GN=CHIP PE=1 SV... [more]
Q9WUD16.9e-4436.36E3 ubiquitin-protein ligase CHIP OS=Mus musculus OX=10090 GN=Stub1 PE=1 SV=1[more]
Q9UNE79.0e-4436.01E3 ubiquitin-protein ligase CHIP OS=Homo sapiens OX=9606 GN=STUB1 PE=1 SV=2[more]
Q5ZHY52.9e-4235.54E3 ubiquitin-protein ligase CHIP OS=Gallus gallus OX=9031 GN=STUB1 PE=2 SV=1[more]
Q8VD331.1e-1233.94Small glutamine-rich tetratricopeptide repeat-containing protein beta OS=Mus mus... [more]
Match NameE-valueIdentityDescription
A0A6J1K6X79.1e-14087.94E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita maxima OX=3661 GN=LOC11149122... [more]
A0A6J1EYU49.1e-14087.94E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111437... [more]
A0A0A0LBQ52.0e-13987.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G634310 PE=4 SV=1[more]
A0A6J1HID65.0e-13887.14E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111463... [more]
A0A6J1HQG31.5e-13786.79E3 ubiquitin-protein ligase CHIP-like isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
Match NameE-valueIdentityDescription
AT3G07370.14.1e-10066.79carboxyl terminus of HSC70-interacting protein [more]
AT3G61390.21.2e-1130.94RING/U-box superfamily protein [more]
AT2G45920.12.7e-1126.96U-box domain-containing protein [more]
AT2G42810.11.9e-0928.23protein phosphatase 5.2 [more]
AT2G42810.21.9e-0928.23protein phosphatase 5.2 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 80..113
e-value: 2.1
score: 17.3
coord: 12..45
e-value: 2.9E-4
score: 30.2
coord: 46..79
e-value: 1.8E-4
score: 30.8
IPR019734Tetratricopeptide repeatPFAMPF13181TPR_8coord: 19..44
e-value: 0.0018
score: 18.3
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 12..45
score: 10.1484
IPR003613U box domainSMARTSM00504Ubox_2coord: 205..268
e-value: 4.9E-24
score: 95.9
IPR003613U box domainPFAMPF04564U-boxcoord: 202..274
e-value: 1.6E-26
score: 92.3
IPR003613U box domainPROSITEPS51698U_BOXcoord: 201..275
score: 31.218241
NoneNo IPR availablePFAMPF12895ANAPC3coord: 48..106
e-value: 2.2E-7
score: 31.0
NoneNo IPR availablePANTHERPTHR46803:SF3OS08G0113300 PROTEINcoord: 7..278
NoneNo IPR availablePANTHERPTHR46803E3 UBIQUITIN-PROTEIN LIGASE CHIPcoord: 7..278
NoneNo IPR availableCDDcd16654RING-Ubox_CHIPcoord: 202..268
e-value: 5.46401E-42
score: 136.606
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 198..276
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 6..142
e-value: 6.7E-32
score: 112.1
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 10..133
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 200..278
e-value: 3.8E-26
score: 92.8

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Sed0023145Sed0023145gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0023145.1-five_prime_utrSed0023145.1-five_prime_utr-LG05:1285891..1286104five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0023145.1-exonSed0023145.1-exon-LG05:1285891..1286215exon
Sed0023145.1-exonSed0023145.1-exon-LG05:1286505..1286572exon
Sed0023145.1-exonSed0023145.1-exon-LG05:1286688..1286751exon
Sed0023145.1-exonSed0023145.1-exon-LG05:1287235..1287306exon
Sed0023145.1-exonSed0023145.1-exon-LG05:1288780..1288913exon
Sed0023145.1-exonSed0023145.1-exon-LG05:1289712..1289865exon
Sed0023145.1-exonSed0023145.1-exon-LG05:1289960..1290070exon
Sed0023145.1-exonSed0023145.1-exon-LG05:1290167..1290580exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0023145.1-cdsSed0023145.1-cds-LG05:1286105..1286215CDS
Sed0023145.1-cdsSed0023145.1-cds-LG05:1286505..1286572CDS
Sed0023145.1-cdsSed0023145.1-cds-LG05:1286688..1286751CDS
Sed0023145.1-cdsSed0023145.1-cds-LG05:1287235..1287306CDS
Sed0023145.1-cdsSed0023145.1-cds-LG05:1288780..1288913CDS
Sed0023145.1-cdsSed0023145.1-cds-LG05:1289712..1289865CDS
Sed0023145.1-cdsSed0023145.1-cds-LG05:1289960..1290070CDS
Sed0023145.1-cdsSed0023145.1-cds-LG05:1290167..1290292CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0023145.1-three_prime_utrSed0023145.1-three_prime_utr-LG05:1290293..1290580three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Sed0023145.1Sed0023145.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0071218 cellular response to misfolded protein
biological_process GO:0045862 positive regulation of proteolysis
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006515 protein quality control for misfolded or incompletely synthesized proteins
biological_process GO:0009737 response to abscisic acid
biological_process GO:0009651 response to salt stress
biological_process GO:0009266 response to temperature stimulus
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0051087 chaperone binding
molecular_function GO:0061630 ubiquitin protein ligase activity