Homology
BLAST of Sed0014960.3 vs. NCBI nr
Match:
XP_023551402.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2911.3 bits (7546), Expect = 0.0e+00
Identity = 1465/1687 (86.84%), Postives = 1555/1687 (92.18%), Query Frame = 0
Query: 1 MGRRKQKKPHRSGGIRVEDHVNPKTQVDINGVVETVESSDIKLDVENEAFFVEVDRTGWN 60
MGRRKQK+PHRSGGIR ED VNPKTQVDI+G+VETVESSDIKLD NE FFVEVDRTGW+
Sbjct: 1 MGRRKQKQPHRSGGIRFEDPVNPKTQVDIHGIVETVESSDIKLDEVNEPFFVEVDRTGWH 60
Query: 61 SNEHHDISEVVLMDIRLEPHFVGFRLDESFCESFKYSLRFQLSNVNEFLLGRIKFGQWPV 120
SNEHHDISEVVLMDIRLE FVG R +E FCES +YSLRF+L N N FLL RIKFG WPV
Sbjct: 61 SNEHHDISEVVLMDIRLEHPFVGIRSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120
Query: 121 LSSSDISLELIERGIEEDKNACSVVLSGNFDGPDEAISGLVHLANLKFMTLRPVMGVTFS 180
LSS++ISLELIER +EED N C VVLSG+FDGPDEAISGLVHLANLKF+TLRPVMGV+FS
Sbjct: 121 LSSNEISLELIERRVEEDMNGCLVVLSGSFDGPDEAISGLVHLANLKFVTLRPVMGVSFS 180
Query: 181 HNIGSLRLRVELLSSAFDACESIFDSSRQLWKKSMMSTIAWLRPEVVSSEAKYGIAESTN 240
N+GSLRLRVE+LSSAFDAC SI DS RQLWKKSMM+TIAWLRPEVVSSE KYG+ ESTN
Sbjct: 181 QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVESTN 240
Query: 241 MDSRLHHEMREDASDSRKHVNFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
MD+ LH E +DAS SRKHVNFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 MDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRMFYNPFSGNLTLNAEQILPHVYG 360
WMVQREKGFS N GVG+ +QLISPLCM LECLDT SRMFYNPFSG+LT NAEQ LPHVYG
Sbjct: 301 WMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQTLPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPEFTRLKRERVECVC 420
GILADEMGLGKTIELLACILSH M FE KGFDDE+QQ VG+QR +F RLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHPMSVFEDGKGFDDEMQQLVGDQRTKFKRLKRERVECVC 420
Query: 421 GAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRKEKRNKKNTVNI 480
GAVSENH Y+GLWVQCDICDAWQHADC+GYSPKGR+L+P+D + GN RK+KRNK+NTVN+
Sbjct: 421 GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRVLEPVDTKDGNCRKDKRNKRNTVNV 480
Query: 481 TVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTCPDSMKLLVYEG 540
TVRDEEHVCM CLELMQATD+PM TGATLIVCPAPILFQW AEILRHT P S+KLLVYEG
Sbjct: 481 TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540
Query: 541 VRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VR TS S+TL VQIND+INSDIVLTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541 VRDTSLSNTLPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDL+GLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLFGLLRFVKA 660
Query: 661 SPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDELQLPPQVEQVTWL 720
SPFNVHRWW EVIRDPYERRDPGAMEFTH FFK+IMWRSLK+HVTDELQLPPQ EQVTWL
Sbjct: 661 SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDVPSDLLITHADAGKLL 780
TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKVP VSSDVPSD+LITHADAGKLL
Sbjct: 721 TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQYPMTMEEILMVLVSKTKIEGEEALRRSVVALNALS 840
+TLLKLRQACCHPQVGSSGLRSLQQ PMTMEEILMVLVSKTKIEGEEALRR+VVALNAL+
Sbjct: 781 NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840
Query: 841 GIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAADQSQSPLK 900
GIAIIEKKFSEA SLYKEALELAEENNEDFR+DPLLSIHIHHNLAEILPLA DQ QSPLK
Sbjct: 841 GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900
Query: 901 NQTYSRSCEVKAYGMDTSENYDNHIMKKQKVSDTLYAPCGNTEKIEDNPLELTKKDTNTK 960
+Q Y R+CEVKA MD SE DNHI+KKQKV+ TLY PC +TEKI D+PLELT +D N K
Sbjct: 901 DQFYPRTCEVKASKMDDSEKSDNHIIKKQKVNGTLYVPCSDTEKIIDSPLELTGQDENAK 960
Query: 961 KEENYELHQSSSYFNDNSLSQACETMRQKYLAVFSSKLSMAQQEFTKSYVQVCSELKERE 1020
KEENYE H+SSSYFNDN L +ACE MRQKYLAVFSSKLS+AQQEFTKSYVQVC+ELK RE
Sbjct: 961 KEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCNELKSRE 1020
Query: 1021 NLKEVWWLEAVQYAEQNKDFSHELIRKIEEAVSGNLNSSKSSRVGSRFRSINALKYHVQT 1080
NL EVWWL+AV +AEQNKDFSHELIRKIEEAVSGNLN+SK SRVGSRFRSI+ALKYHVQT
Sbjct: 1021 NLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKYHVQT 1080
Query: 1081 GLDLLEASRKVVLDRLLEIDQTMDNPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1140
GLDLLEASRK+VL RLLEIDQTM+NPK+EDIERVRYCQNCQADSNGPPCVLCELDELFQE
Sbjct: 1081 GLDLLEASRKLVLGRLLEIDQTMENPKDEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1140
Query: 1141 YEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSKVGNEESNKRD 1200
YEARLFRLN VQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSS+ G EESNKRD
Sbjct: 1141 YEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEESNKRD 1200
Query: 1201 VGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIGAANKQLHLLEYMRKEYGHARSLAIA 1260
GE+V+VSKHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARSLAIA
Sbjct: 1201 AGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIA 1260
Query: 1261 QAQVLNAHDEIKMATTRLCLWDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLSRVKG 1320
QAQVLNAHDEIKMATTRLCL NDDD SAYAISEHELPAASVQYSSDKF+SLAMLSRVKG
Sbjct: 1261 QAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSDKFVSLAMLSRVKG 1320
Query: 1321 KLRYLKGLVQSKQNIP---SNNLRLAQEPAITSTSMEQKSENTSKAYEESCPVCQEKLSN 1380
KLRYLKGLVQSKQNIP S+N Q P ITSTS+EQKSENT KA EESCPVCQE LSN
Sbjct: 1321 KLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQEALSN 1380
Query: 1381 QKMVFQCGHMTCCKCLFAMTEKTVHDSKIRTKWVMCPTCRQHTDFGNIAYVDDSKNETLD 1440
QKMVFQCGHMTCCKCLFAMTEK + DS I+TKWVMCPTCRQHTDFGNIAY DDSKNETLD
Sbjct: 1381 QKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLD 1440
Query: 1441 PSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYA 1500
PSTLH+TSRE E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYA
Sbjct: 1441 PSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYA 1500
Query: 1501 FSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRASAQVPELRSAQVLLLLIQHGA 1560
F+AN ITF+RMKGGRKSHTAISEFRGQ+I+ KEN KK+ A+VPELRSAQVLLLLIQHGA
Sbjct: 1501 FAANNITFVRMKGGRKSHTAISEFRGQKINTKENHKKQPLAEVPELRSAQVLLLLIQHGA 1560
Query: 1561 NGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRS 1620
NGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH+LNRS
Sbjct: 1561 NGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHRLNRS 1620
Query: 1621 RGSCSFITGNAKNQDQPFLTLKEVESLFVSKPSPLEESDEKGSETLRHLAPSMAATIAAE 1680
R S SFITGN KNQDQP TLKEVESLF S+PSPL ESDEK SETLRHLAPSMAATIAAE
Sbjct: 1621 RESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAATIAAE 1680
Query: 1681 RRLRDHT 1685
RRL++HT
Sbjct: 1681 RRLKEHT 1686
BLAST of Sed0014960.3 vs. NCBI nr
Match:
XP_038884903.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida] >XP_038884904.1 E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida])
HSP 1 Score: 2907.9 bits (7537), Expect = 0.0e+00
Identity = 1478/1691 (87.40%), Postives = 1558/1691 (92.13%), Query Frame = 0
Query: 1 MGRRKQKKPHRSGGIRVEDHVNPKTQVDINGVVETVESSDIKLDVENEAFFVEVDRTGWN 60
MGRRKQ++PHRSGGIR ED +PKTQ+DING VESSDIK+ NE FVEVDRTGWN
Sbjct: 1 MGRRKQRQPHRSGGIRFEDRGDPKTQIDING---AVESSDIKVAEVNEPIFVEVDRTGWN 60
Query: 61 SNEHHDISEVVLMDIRLEPHFVGFRLDESFCESFKYSLRFQLSNVNEFLLGRIKFGQWPV 120
SNEHHDISEV LMD+RLE FV FRLD+SFCE+ +YSLRF+L NVN LL RIKFG WPV
Sbjct: 61 SNEHHDISEVFLMDVRLEHPFVDFRLDKSFCENSRYSLRFRLCNVNGSLLDRIKFGHWPV 120
Query: 121 LSSSDISLELIERGIEEDKNACSVVLSGNFDGPDEAISGLVHLANLKFMTLRPVMGVTFS 180
LSS+DI LELIER +EED ACSVVLSGNFDGPDEAISGLVHLANLKF TLRPV GV+ S
Sbjct: 121 LSSNDILLELIERDMEEDMKACSVVLSGNFDGPDEAISGLVHLANLKFATLRPVEGVSVS 180
Query: 181 HNIGSLRLRVELLSSAFDACESIFDSSRQLWKKSMMSTIAWLRPEVVSSEAKYGIAESTN 240
N GSLRLRVE+LSSAF ACESIFD+ RQLWKKSMM+TIAWLRPEVV SE KYG+ +STN
Sbjct: 181 QNTGSLRLRVEILSSAFAACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKSTN 240
Query: 241 MDSRLHHEMREDASDSRKHVNFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
MD+ LHH +D S+SRKHVNFDTV FYDAIKPSKD+PMLDEDIPNLLPKLR YQRRAAY
Sbjct: 241 MDTSLHHGTGDDTSNSRKHVNFDTVAFYDAIKPSKDEPMLDEDIPNLLPKLRQYQRRAAY 300
Query: 301 WMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRMFYNPFSGNLTLNAEQILPHVYG 360
WMVQREKGFSGN+GVG+ +QLISPLCM L+C+DTCSR+FYNPFSGNLTLNAEQ LPHVYG
Sbjct: 301 WMVQREKGFSGNLGVGENVQLISPLCMALKCVDTCSRVFYNPFSGNLTLNAEQTLPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPEFTRLKRERVECVC 420
GILADEMGLGKTIELLACILSH+M FEG KGF DELQQ V +QR EF RLKRERVEC+C
Sbjct: 361 GILADEMGLGKTIELLACILSHQMSVFEGGKGFHDELQQPVEDQRTEFKRLKRERVECLC 420
Query: 421 GAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRKEKRNKKNTVNI 480
GAVSENH YKGLWVQCDICDAWQHADC+GYSPKGRILKPID E GN RKEKRN +NT+N+
Sbjct: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRILKPIDTEGGNCRKEKRNNRNTLNV 480
Query: 481 TVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTCPDSMKLLVYEG 540
VR EEHVC CLELMQATD+PMATGATLIVCPAPILFQW+AEILRHT P S+KLLVYEG
Sbjct: 481 IVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYEG 540
Query: 541 VRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VR TS STLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP
Sbjct: 541 VRDTSLLSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDELQLPPQVEQVTWL 720
SPFNVHRWW EVIRDPYERRDPGAMEFTH FFK+IMWRSLKIHVTDELQLPPQ EQVTWL
Sbjct: 661 SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDVPSDLLITHADAGKLL 780
TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVK+KVPD +SSD+PSDLLI HADAGKLL
Sbjct: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKKKVPDCISSDIPSDLLINHADAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQYPMTMEEILMVLVSKTKIEGEEALRRSVVALNALS 840
STLLKLRQACCHPQVGSSGLRSLQQ PMTMEEILMVLVSKTKIEGEEALRRSVVALNAL+
Sbjct: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
Query: 841 GIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAADQSQSPLK 900
GIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLA DQSQS LK
Sbjct: 841 GIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVDQSQSRLK 900
Query: 901 NQTYSRSCEVKAYGMDTSENYDN--HIMKKQKVSDTLYAPCG--NTEKIEDNPLELTKKD 960
+Q R+ EVKA MD SE YDN H+MKKQKVS+TLYAPC NT K+ D PLELT+KD
Sbjct: 901 DQFCPRTSEVKAARMDDSEKYDNHVHVMKKQKVSETLYAPCSEDNTGKMIDRPLELTRKD 960
Query: 961 TNTKKEENYELHQSSSYFNDNSLSQACETMRQKYLAVFSSKLSMAQQEFTKSYVQVCSEL 1020
TNTKKEEN E H SSSYF+D S+ +ACE MRQKYLAVFSSKLS+AQQEFTKSY+QV SEL
Sbjct: 961 TNTKKEENCEPHLSSSYFDDISIRKACEAMRQKYLAVFSSKLSVAQQEFTKSYMQVGSEL 1020
Query: 1021 KERENLKEVWWLEAVQYAEQNKDFSHELIRKIEEAVSGNLNSSKSSRVGSRFRSINALKY 1080
K+R NL VWWLEAV +AEQNKDFSHELIRK+EEAVSGNLN+SK SRVGSRFRSINALKY
Sbjct: 1021 KDRGNLNHVWWLEAVHHAEQNKDFSHELIRKVEEAVSGNLNNSK-SRVGSRFRSINALKY 1080
Query: 1081 HVQTGLDLLEASRKVVLDRLLEIDQTMDNPKEEDIERVRYCQNCQADSNGPPCVLCELDE 1140
HVQ+GLDLLEASRKVVLDRLLEIDQTM+NPKEEDIERVRYC+NCQADSNGPPCVLCELD+
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDD 1140
Query: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSKVGNEES 1200
LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKN SSS+VG EE
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSRVGYEEP 1200
Query: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIGAANKQLHLLEYMRKEYGHARS 1260
NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
Query: 1261 LAIAQAQVLNAHDEIKMATTRLCLWDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLS 1320
LAIAQAQVLNAHDEIKMATTRLCL D DDDSSAYAISEHELPAASVQYSSDKFMSLAML+
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRD-DDDSSAYAISEHELPAASVQYSSDKFMSLAMLA 1320
Query: 1321 RVKGKLRYLKGLVQSKQNIP---SNNLRLAQEPAITSTSMEQKSENTSKAYEESCPVCQE 1380
RVKGKLRYLKGLVQSKQNIP S+NL L Q+PA STSMEQKSENTSKA EESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLDSSSNLALTQQPATMSTSMEQKSENTSKADEESCPVCQE 1380
Query: 1381 KLSNQKMVFQCGHMTCCKCLFAMTEKTVHDSKIRTKWVMCPTCRQHTDFGNIAYVDDSKN 1440
KLSNQKMVFQCGH+TCCKCLFAMTEKT+H SKI+TKWVMCPTCRQHTDFGNIAY DDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
Query: 1441 ETLDPSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500
ETLDPSTLHETSREHE SITVQGSYGTK+EAVVRRILWIKYTDSKAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLHETSREHELSITVQGSYGTKVEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500
Query: 1501 LQYAFSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRASAQVPELRSAQVLLLLI 1560
LQYAF+AN ITFIRMKGGR+SHTAISEFRGQ+I+AKEN KKR S +VPELRSAQVLLLLI
Sbjct: 1501 LQYAFAANSITFIRMKGGRQSHTAISEFRGQKINAKENQKKRPSGKVPELRSAQVLLLLI 1560
Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHK 1620
QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESI+K
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQKNKTFVHRFIVKDTVEESIYK 1620
Query: 1621 LNRSRGSCSFITGNAKNQDQPFLTLKEVESLFVSKPSPLEESDEKGSETLRHLAPSMAAT 1680
LNRSR S SFITGN KNQDQP LTLKEVESLF SK SPL E+DEKGSETLRHLAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNTKNQDQPLLTLKEVESLFASKASPLRENDEKGSETLRHLAPSMAAT 1680
Query: 1681 IAAERRLRDHT 1685
IAAERRL+ HT
Sbjct: 1681 IAAERRLKVHT 1686
BLAST of Sed0014960.3 vs. NCBI nr
Match:
XP_022134046.1 (E3 ubiquitin-protein ligase SHPRH [Momordica charantia] >XP_022134047.1 E3 ubiquitin-protein ligase SHPRH [Momordica charantia])
HSP 1 Score: 2901.7 bits (7521), Expect = 0.0e+00
Identity = 1464/1688 (86.73%), Postives = 1555/1688 (92.12%), Query Frame = 0
Query: 1 MGRRKQKKPHRSGGIRVEDHVNPKTQVDINGVVETVESSDIKLDVENEAFFVEVDRTGWN 60
MGRRKQ +PHRSGGIR+E H NP+TQVDI+ V+ET +SSDIKL+ NE F+VEVD TGW
Sbjct: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDISVVLETEKSSDIKLNEINEPFYVEVDPTGWK 60
Query: 61 SNEHHDISEVVLMDIRLEPHFVGFRLDESFCESFKYSLRFQLSNVNEFLLGRIKFGQWPV 120
SN+H DISEVVLMD+R E HFVGF+ D+SF ES +YSLRF+L NVNEFLLGRIKFG WP+
Sbjct: 61 SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
Query: 121 LSSSDISLELIERGIEEDKNACSVVLSGNFDGPDEAISGLVHLANLKFMTLRPVMGVTFS 180
LSSSDISLELI++G+EE +ACSVVLSGNFDGPD+AISGLVHLANLKFMTLRPVMG T S
Sbjct: 121 LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
Query: 181 HNIGSLRLRVELLSSAFDACESIFDSSRQLWKKSMMSTIAWLRPEVVSSEAKYGIAESTN 240
+ GSLRLRVE+LSSA DACESIFD+ RQLWKKSMMSTIAWLRPEV SSEAKYG+ +STN
Sbjct: 181 RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
Query: 241 MDSRLHHEMREDASDSRKHVNFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
+DS HEM + +S++RKH+NFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRMFYNPFSGNLTLNAEQILPHVYG 360
WMVQREK FSGN GVG+ +QLISPLC+ LECLDTC+RMFYNPFSGNLTL+AE+ LPHVYG
Sbjct: 301 WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPEFTRLKRERVECVC 420
GILADEMGLGKTIELLACILSHRM FEG KGF +E QQ VG QR EF RLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
Query: 421 GAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRKEKRNKKNTVNI 480
GAVSENH YKGLWVQCDICDAWQHADC+GYSPKGR+L+PID E GN K+KRNKKN VN+
Sbjct: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLRPIDTEGGNCIKKKRNKKNRVNV 480
Query: 481 TVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTCPDSMKLLVYEG 540
VRD +HVC+ CLELMQATD PMAT ATLIVCPAPILFQW AEILRHT P S+KLLVYEG
Sbjct: 481 VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
Query: 541 VRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VR TS SSTLSVQINDL+NSDI+LTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP
Sbjct: 541 VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDELQLPPQVEQVTWL 720
SPF+V+RWW EVIRDPYERRDPGAMEFTH FFK+IMWRSLKIHVTDELQLPPQ EQVTWL
Sbjct: 661 SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDVPSDLLITHADAGKLL 780
TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKV D VSSD PSD+LITH DAGKLL
Sbjct: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQYPMTMEEILMVLVSKTKIEGEEALRRSVVALNALS 840
STLLKLRQACCHPQVGSSGLRSLQQ PMTMEEILMVLVSKTKIEGEEALRRSVVALNAL+
Sbjct: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
Query: 841 GIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAADQSQSPLK 900
GIAIIEKKFSEAFSLYKEALELAE NNEDFRLDPLLSIHIHHNLAEILP A DQSQSPLK
Sbjct: 841 GIAIIEKKFSEAFSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
Query: 901 NQTYSRSCEVKAYGMDTSENYDNHIMKKQKVSDTLYAPCGNTEKIEDNPLELTKKDTNTK 960
+Q+YSR+CEVKA MD SENYDNH MKKQKVS+T YAPC +TEKI DNPL
Sbjct: 901 DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL---------- 960
Query: 961 KEENYELHQSSSYFNDNSLSQACETMRQKYLAVFSSKLSMAQQEFTKSYVQVCSELKERE 1020
+ H+SSSYFND+S +ACETMRQKYLAVFSSKLS+AQQEFTKS++QVCSEL+ERE
Sbjct: 961 -----KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCSELRERE 1020
Query: 1021 NLKEVWWLEAVQYAEQNKDFSHELIRKIEEAVSGNLNSSKSSRVGSRFRSINALKYHVQT 1080
+L +VWWLEAV +AEQNKDF HELIRKIEEAVSGN+N+S+SSRVGSRFRSINALKYHVQT
Sbjct: 1021 SLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQT 1080
Query: 1081 GLDLLEASRKVVLDRLLEIDQTMDNPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1140
GLDLLEASR VVLDRLLEIDQTM+NPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQE
Sbjct: 1081 GLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1140
Query: 1141 YEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSKVGNEESNKRD 1200
YEARLFRLNKVQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKNLSSS+VG EESNKRD
Sbjct: 1141 YEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRD 1200
Query: 1201 VGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIGAANKQLHLLEYMRKEYGHARSLAIA 1260
VGEKVMV KHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARSLAIA
Sbjct: 1201 VGEKVMVCKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIA 1260
Query: 1261 QAQVLNAHDEIKMATTRLCLWDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLSRVKG 1320
QAQVLNAHDEIKMATTRLCL DNDDD SAYAISEHELPAASVQYSSDKF+SLAMLSRVKG
Sbjct: 1261 QAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKG 1320
Query: 1321 KLRYLKGLVQSKQNIP---SNNLRLAQEPAITSTSMEQKSENTSKAYEESCPVCQEKLSN 1380
KLRYLKGLVQSKQN P S+NL L QEPAI STSMEQKSENT KA EESCPVCQEKLSN
Sbjct: 1321 KLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSN 1380
Query: 1381 QKMVFQCGHMTCCKCLFAMTEKTVHDSKIRTKWVMCPTCRQHTDFGNIAYVDDSKNETLD 1440
QKMVFQCGHM CCKCLFAMTEKT+ DSKI+T+WVMCPTCRQHTDFGNIA+ DDSKNE LD
Sbjct: 1381 QKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILD 1440
Query: 1441 PSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYA 1500
PSTLHETSREHET ITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYA
Sbjct: 1441 PSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYA 1500
Query: 1501 FSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRASAQVPELRSAQVLLLLIQHGA 1560
F+ANGITFIRMKGGRKS TAISEFRGQ+I+AKEN KKR AQVPEL+SAQVLLLLIQHGA
Sbjct: 1501 FAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQVLLLLIQHGA 1560
Query: 1561 NGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRS 1620
NGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRS
Sbjct: 1561 NGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRS 1620
Query: 1621 RGSCSFITGNAKNQDQPFLTLKEVESLFVSKPSPLEESDEKGSETLRHLAPSMAATIAAE 1680
R + SFITGNAKNQDQPFLTLKEVESLF SK SPL +SD+K SETLRHLAPSMAA +AAE
Sbjct: 1621 RENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAE 1671
Query: 1681 RRLRDHTA 1686
RRLR+HTA
Sbjct: 1681 RRLREHTA 1671
BLAST of Sed0014960.3 vs. NCBI nr
Match:
XP_022938761.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita moschata])
HSP 1 Score: 2899.8 bits (7516), Expect = 0.0e+00
Identity = 1461/1687 (86.60%), Postives = 1552/1687 (92.00%), Query Frame = 0
Query: 1 MGRRKQKKPHRSGGIRVEDHVNPKTQVDINGVVETVESSDIKLDVENEAFFVEVDRTGWN 60
MGRRKQK+PHRSGGIR ED V+PKTQVDING+VETVESSDIKLD NE FVEVDRTGW+
Sbjct: 1 MGRRKQKQPHRSGGIRFEDPVSPKTQVDINGIVETVESSDIKLDEVNEPLFVEVDRTGWH 60
Query: 61 SNEHHDISEVVLMDIRLEPHFVGFRLDESFCESFKYSLRFQLSNVNEFLLGRIKFGQWPV 120
SNEHHDISEVVLMDIRLE FVG + +E FCES +YSLRF+L N N FLL RIKFG WPV
Sbjct: 61 SNEHHDISEVVLMDIRLEHPFVGIQSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120
Query: 121 LSSSDISLELIERGIEEDKNACSVVLSGNFDGPDEAISGLVHLANLKFMTLRPVMGVTFS 180
LSS+DISLELIER +EED N CSVVLSG+FDGPDEAISGLVHLA+LKF+TLRPVMGV+FS
Sbjct: 121 LSSNDISLELIERRVEEDMNGCSVVLSGSFDGPDEAISGLVHLAHLKFVTLRPVMGVSFS 180
Query: 181 HNIGSLRLRVELLSSAFDACESIFDSSRQLWKKSMMSTIAWLRPEVVSSEAKYGIAESTN 240
N+GSLRLRVE+LSSAFDAC SI DS RQLWKKSMM+TIAWLRPEVVSSE KYG+ STN
Sbjct: 181 QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVGSTN 240
Query: 241 MDSRLHHEMREDASDSRKHVNFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
MD+ LH E +DAS SRKHVNFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 MDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRMFYNPFSGNLTLNAEQILPHVYG 360
WMVQREKGFS N GVG+ +QLISPLCM LECLDT SRMFYNPFSG+LT NAEQILPHVYG
Sbjct: 301 WMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQILPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPEFTRLKRERVECVC 420
GILADEMGLGKTIELLACILSHRM FE KG DDELQ VG+QR +F RLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHRMSVFESGKGLDDELQPLVGDQRTKFKRLKRERVECVC 420
Query: 421 GAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRKEKRNKKNTVNI 480
GAVSENH Y+GLWVQCDICDAWQHADC+GYSPKGRIL+P+D + GN RK+KRNK+NTVN+
Sbjct: 421 GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKRNKRNTVNV 480
Query: 481 TVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTCPDSMKLLVYEG 540
TVRDEEHVCM CLELMQATD+PM TGATLIVCPAPILFQW AEILRHT P S+KLLVYEG
Sbjct: 481 TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540
Query: 541 VRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VR TS S+T VQIND+INSDIVLTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541 VRDTSLSNTSPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDELQLPPQVEQVTWL 720
SPFNVHRWW EVIRDPYERRDPGAMEFTH FFK+IMWRSLK+HVTDELQLPPQ EQVTWL
Sbjct: 661 SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDVPSDLLITHADAGKLL 780
TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKVP VSSDVPSD+LITHADAGKLL
Sbjct: 721 TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQYPMTMEEILMVLVSKTKIEGEEALRRSVVALNALS 840
+TLLKLRQACCHPQVGSSGLRSLQQ PMTMEEILMVLVSKTKIEGEEALRR+VVALNAL+
Sbjct: 781 NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840
Query: 841 GIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAADQSQSPLK 900
GIAIIEKKFSEA SLYKEALELAEENNEDFR+DPLLSIHIHHNLAEILPLA DQ QSPLK
Sbjct: 841 GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900
Query: 901 NQTYSRSCEVKAYGMDTSENYDNHIMKKQKVSDTLYAPCGNTEKIEDNPLELTKKDTNTK 960
+Q Y R+CEVKA MD SE DNHI+KKQKV+ TLYAPC +TEKI D+PLELT D N K
Sbjct: 901 DQFYPRTCEVKASKMDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAK 960
Query: 961 KEENYELHQSSSYFNDNSLSQACETMRQKYLAVFSSKLSMAQQEFTKSYVQVCSELKERE 1020
KEENYE H+SSSYFNDN L +ACE MRQKYLAVFSSKL +AQQEFTKSYVQVC+ELK RE
Sbjct: 961 KEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVCNELKSRE 1020
Query: 1021 NLKEVWWLEAVQYAEQNKDFSHELIRKIEEAVSGNLNSSKSSRVGSRFRSINALKYHVQT 1080
NL EVWWL+AV +AEQNKDFSHELIRKIEEAVSGNLN+SK SRVGSRFRSI+ALKYHVQT
Sbjct: 1021 NLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKYHVQT 1080
Query: 1081 GLDLLEASRKVVLDRLLEIDQTMDNPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1140
GLDLLEASRK+V+ RLLEIDQTM++PK+EDIERVRYCQNCQADSNGPPCVLCELDELFQE
Sbjct: 1081 GLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1140
Query: 1141 YEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSKVGNEESNKRD 1200
YEARLFRLN VQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSS+ G EESNKRD
Sbjct: 1141 YEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEESNKRD 1200
Query: 1201 VGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIGAANKQLHLLEYMRKEYGHARSLAIA 1260
GE+V+VSKHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARSLAIA
Sbjct: 1201 AGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIA 1260
Query: 1261 QAQVLNAHDEIKMATTRLCLWDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLSRVKG 1320
QAQVLNAHDEIKMATTRLCL NDDD SAYAISEHELPAASVQYSS+KF+SLA LSRVKG
Sbjct: 1261 QAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSNKFVSLATLSRVKG 1320
Query: 1321 KLRYLKGLVQSKQNIP---SNNLRLAQEPAITSTSMEQKSENTSKAYEESCPVCQEKLSN 1380
KLRYLKGLVQSKQNIP S+N Q P ITSTS+EQKSENT KA EESCPVCQE LSN
Sbjct: 1321 KLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQETLSN 1380
Query: 1381 QKMVFQCGHMTCCKCLFAMTEKTVHDSKIRTKWVMCPTCRQHTDFGNIAYVDDSKNETLD 1440
QKMVFQCGHMTCCKCLFAMTEK + DS I+TKWVMCPTCRQHTDFGNIAY DDSKNETLD
Sbjct: 1381 QKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLD 1440
Query: 1441 PSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYA 1500
PSTLH+TSRE E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYA
Sbjct: 1441 PSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYA 1500
Query: 1501 FSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRASAQVPELRSAQVLLLLIQHGA 1560
F+AN ITF+RMKGGRKSHTAISEFRGQ+I+AKEN KK+ A+VPE+RSAQVLLLLIQHGA
Sbjct: 1501 FAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLIQHGA 1560
Query: 1561 NGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRS 1620
NGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH+LNRS
Sbjct: 1561 NGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHRLNRS 1620
Query: 1621 RGSCSFITGNAKNQDQPFLTLKEVESLFVSKPSPLEESDEKGSETLRHLAPSMAATIAAE 1680
R S SFITGN KNQDQP TLKEVESLF S+PSPL ESDEK SETLRHLAPSMAATIAAE
Sbjct: 1621 RESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAATIAAE 1680
Query: 1681 RRLRDHT 1685
RRL++HT
Sbjct: 1681 RRLKEHT 1686
BLAST of Sed0014960.3 vs. NCBI nr
Match:
KAG6578487.1 (E3 ubiquitin-protein ligase SHPRH, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 2894.4 bits (7502), Expect = 0.0e+00
Identity = 1458/1687 (86.43%), Postives = 1550/1687 (91.88%), Query Frame = 0
Query: 1 MGRRKQKKPHRSGGIRVEDHVNPKTQVDINGVVETVESSDIKLDVENEAFFVEVDRTGWN 60
MGRRKQK+PHRSGGIR ED VNPKTQVDING+VETVESSDIKLD NE FVEVDRTGW+
Sbjct: 1 MGRRKQKQPHRSGGIRFEDPVNPKTQVDINGIVETVESSDIKLDEVNEPLFVEVDRTGWH 60
Query: 61 SNEHHDISEVVLMDIRLEPHFVGFRLDESFCESFKYSLRFQLSNVNEFLLGRIKFGQWPV 120
SNEHHDISEVVLMDIRLE FVG + +E FCES +YSLRF+L N N FLL RIKFG WPV
Sbjct: 61 SNEHHDISEVVLMDIRLEHPFVGIQSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120
Query: 121 LSSSDISLELIERGIEEDKNACSVVLSGNFDGPDEAISGLVHLANLKFMTLRPVMGVTFS 180
LSS+DISLELIER +EED N CSVVLSG+FDGP EAISGLVHLA+LKF+TLRPVMGV+FS
Sbjct: 121 LSSNDISLELIERHVEEDMNGCSVVLSGSFDGPYEAISGLVHLAHLKFVTLRPVMGVSFS 180
Query: 181 HNIGSLRLRVELLSSAFDACESIFDSSRQLWKKSMMSTIAWLRPEVVSSEAKYGIAESTN 240
N+GSLRLRVE+LSSAFDAC SI DS RQLWKKSMM+TIAWLRPEVVSSE KYG+ STN
Sbjct: 181 QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVVSTN 240
Query: 241 MDSRLHHEMREDASDSRKHVNFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
MD+ LH E +DAS SRKHVNFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 MDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRMFYNPFSGNLTLNAEQILPHVYG 360
WMVQREKGFS N GVG+ +QLISPLCM LECLDT SRMFYNPFSG+LT NAEQILPHVYG
Sbjct: 301 WMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQILPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPEFTRLKRERVECVC 420
GILADEMGLGKTIELLACILSHR+ FE KG DDELQ VG+QR +F RLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHRISVFESGKGLDDELQPLVGDQRTKFKRLKRERVECVC 420
Query: 421 GAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRKEKRNKKNTVNI 480
GAVSENH Y+GLWVQCDICDAWQHADC+GYSPKGRIL+P+D + GN RK+KRNK+NTVN+
Sbjct: 421 GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKRNKRNTVNV 480
Query: 481 TVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTCPDSMKLLVYEG 540
TVRDEEHVCM CLELMQATD+PM TGATLIVCPAPILFQW AEILRHT P S+KLLVYEG
Sbjct: 481 TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540
Query: 541 VRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VR TS S+T VQIND+INSDIVLTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541 VRDTSLSNTSPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDELQLPPQVEQVTWL 720
SPFNVHRWW EVIRDPYERRDPGAMEFTH FFK+IMWRSLK+HVTDELQLPPQ EQVTWL
Sbjct: 661 SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDVPSDLLITHADAGKLL 780
TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKVP VSSDVPSD+LITHADAGKLL
Sbjct: 721 TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQYPMTMEEILMVLVSKTKIEGEEALRRSVVALNALS 840
+TLLKLRQACCHPQVGSSGLRSLQQ PMTMEEILMVLVSKTKIEGEEALRR+VVALNAL+
Sbjct: 781 NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840
Query: 841 GIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAADQSQSPLK 900
GIAIIEKKFSEA SLYKEALELAEENNEDFR+DPLLSIHIHHNLAEILPLA DQ QSPLK
Sbjct: 841 GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900
Query: 901 NQTYSRSCEVKAYGMDTSENYDNHIMKKQKVSDTLYAPCGNTEKIEDNPLELTKKDTNTK 960
+Q Y R+CEVKA MD SE DNHI+KKQKV+ TLYAPC +TEKI D+PLELT D N K
Sbjct: 901 DQFYPRTCEVKASKMDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAK 960
Query: 961 KEENYELHQSSSYFNDNSLSQACETMRQKYLAVFSSKLSMAQQEFTKSYVQVCSELKERE 1020
KEENYE H+SSSYFNDN L +ACE MRQKYLAVFSSKL +AQQEFTKSYVQVC+ELK RE
Sbjct: 961 KEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVCNELKSRE 1020
Query: 1021 NLKEVWWLEAVQYAEQNKDFSHELIRKIEEAVSGNLNSSKSSRVGSRFRSINALKYHVQT 1080
NL EVWWL+AV +AEQNKDFSHELIRKIEEAVSGNLN+SK SRVGSRFRSI+ALKYHVQT
Sbjct: 1021 NLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKYHVQT 1080
Query: 1081 GLDLLEASRKVVLDRLLEIDQTMDNPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1140
GLDLLEASRK+V+ RLLEIDQTM++PK+EDIERVRYCQNCQADSNGPPCVLCELDELFQE
Sbjct: 1081 GLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1140
Query: 1141 YEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSKVGNEESNKRD 1200
YEARLFRLN VQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSS+ G EESNKRD
Sbjct: 1141 YEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEESNKRD 1200
Query: 1201 VGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIGAANKQLHLLEYMRKEYGHARSLAIA 1260
GE+V+VSKHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARSLAIA
Sbjct: 1201 AGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIA 1260
Query: 1261 QAQVLNAHDEIKMATTRLCLWDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLSRVKG 1320
QAQVLNAHDEIKMATTRLCL NDDD SAYAISEHELPAASVQYSSDKF+SLA LSRVKG
Sbjct: 1261 QAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSDKFVSLATLSRVKG 1320
Query: 1321 KLRYLKGLVQSKQNIP---SNNLRLAQEPAITSTSMEQKSENTSKAYEESCPVCQEKLSN 1380
KLRYLKGLVQSKQNIP S+N Q P ITSTS+EQKSENT KA EESCPVCQE LSN
Sbjct: 1321 KLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQETLSN 1380
Query: 1381 QKMVFQCGHMTCCKCLFAMTEKTVHDSKIRTKWVMCPTCRQHTDFGNIAYVDDSKNETLD 1440
QKMVFQCGHMTCCKCLFAMTEK + DS I+TKWVMCPTCRQHTDFGNIAY DDSKNETLD
Sbjct: 1381 QKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLD 1440
Query: 1441 PSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYA 1500
PSTLH+TSRE E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYA
Sbjct: 1441 PSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYA 1500
Query: 1501 FSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRASAQVPELRSAQVLLLLIQHGA 1560
F+AN ITF+RMKGGRKSHTAISEFRGQ+I+AKEN KK+ A+VPE+RSAQVLLLLIQHGA
Sbjct: 1501 FAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLIQHGA 1560
Query: 1561 NGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRS 1620
NGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH+LNRS
Sbjct: 1561 NGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHRLNRS 1620
Query: 1621 RGSCSFITGNAKNQDQPFLTLKEVESLFVSKPSPLEESDEKGSETLRHLAPSMAATIAAE 1680
R S SFI GN KNQDQP TLKEVESLF S+PSPL E+DEK SETLRHLAPS+AATIAAE
Sbjct: 1621 RESSSFIAGNTKNQDQPVFTLKEVESLFASRPSPLRENDEKESETLRHLAPSLAATIAAE 1680
Query: 1681 RRLRDHT 1685
RRL++HT
Sbjct: 1681 RRLKEHT 1686
BLAST of Sed0014960.3 vs. ExPASy Swiss-Prot
Match:
Q149N8 (E3 ubiquitin-protein ligase SHPRH OS=Homo sapiens OX=9606 GN=SHPRH PE=1 SV=2)
HSP 1 Score: 518.5 bits (1334), Expect = 2.9e-145
Identity = 380/1256 (30.25%), Postives = 574/1256 (45.70%), Query Frame = 0
Query: 415 RVECVCGAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRKEKRNK 474
R EC+CG + + + VQC C WQHA C+ Y K +KP
Sbjct: 658 RFECICGELDQID--RKPRVQCLKCHLWQHAKCVNYDEKNLKIKPF-------------- 717
Query: 475 KNTVNITVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTCPDSMK 534
C CL M+ P++T ATLI+ P+ I QW EI RH S++
Sbjct: 718 -------------YCPHCLVAME----PVSTRATLIISPSSICHQWVDEINRHVRSSSLR 777
Query: 535 LLVYEGVRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEG--DRRFMRFQK 594
+LVY+GV+ F +Q + L DIV+ +YDVL+ +L++ H D R +R QK
Sbjct: 778 VLVYQGVKKDGF-----LQPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQK 837
Query: 595 RYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLY 654
RY IP+PL + WWRICLDE QMVE A EMA RL +RWCI+GTP+QR LEDL+
Sbjct: 838 RYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGTPVQRGLEDLF 897
Query: 655 GLLRFVKASPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDELQLPP 714
GL+ F+ P+ V WW ++ PY +++P + ++F +I+WRS K V D++Q+PP
Sbjct: 898 GLVVFLGIEPYCVKHWWVRLLYRPYCKKNP---QHLYSFIAKILWRSAKKDVIDQIQIPP 957
Query: 715 QVEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDVPSDLLIT 774
Q E++ WL FSP+E HFY RQHE C ++ + D +K DR
Sbjct: 958 QTEEIHWLHFSPVERHFYHRQHEVCCQDVVVKLRKISDWALKLSSLDR------------ 1017
Query: 775 HADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQYPMTMEEILMVLVSKTKIEGEEALRRS 834
+L LL+LRQACCHPQ LQ+ MTMEE+L L K E EEA R+
Sbjct: 1018 -RTVTSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQL 1077
Query: 835 VVALNALSGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAA 894
V ALN L+GI II+ +++ A LY+E L +EE+ + D L +H HNL E+L
Sbjct: 1078 VCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELL---- 1137
Query: 895 DQSQSPLKNQTYSRSCEVKAYGMDTSENYDNHIMKKQKVSDTLYAPCGNTEKIEDNPLEL 954
I + + TL
Sbjct: 1138 --------------------------------IARHPGIPPTL----------------- 1197
Query: 955 TKKDTNTKKEENYELHQSSSYFNDNSLSQACETMRQKYLAVFSSKLSMAQQEFTKSYVQV 1014
D L + + +R+ Y++ +++++ AQQ Q
Sbjct: 1198 ----------------------RDGRLEEEAKQLREHYMSKCNTEVAEAQQAL-YPVQQT 1257
Query: 1015 CSELKERENLKEVWWLEAVQYAEQNKDFSHELIRKIEEAVSGNLNSSKSS-RVGSRFRSI 1074
EL+ + + WWL + A + EL++++ ++ N + +FR
Sbjct: 1258 IHELQRKIHSNSPWWLNVIHRAIE-FTIDEELVQRVRNEITSNYKQQTGKLSMSEKFRDC 1317
Query: 1075 NALKYHVQTGLDLLEASRKVVLDRLLEIDQTMDNPKEEDIERVRYCQNCQADSNGPPCVL 1134
L++ + T ++ L +K+V + + ++ P IE C A CV
Sbjct: 1318 RGLQFLLTTQMEELNKCQKLVREAVKNLE---GPPSRNVIESATVCHLRPARLPLNCCVF 1377
Query: 1135 CELDELFQEYEARLFRLNKVQGGMVTSVEEAVEAQK-----KKSALNRFYWSLLQQNKNL 1194
C+ DELF EYE++LF N V+ G EE +E ++ + R W++
Sbjct: 1378 CKADELFTEYESKLFS-NTVK-GQTAIFEEMIEDEEGLVDDRAPTTTRGLWAI------- 1437
Query: 1195 SSSKVGNEESNKRDVGEKVMVSKHPSELEVVLGVIKNFCKT-QLGRESIGAANKQLHLLE 1254
SE E + I +F K+ + E + + + L E
Sbjct: 1438 -------------------------SETERSMKAILSFAKSHRFDVEFVDEGSTSMDLFE 1497
Query: 1255 YMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLCLWD----NDDDSSAYAISEHELPAAS 1314
+KEY +A ++A DE+ MAT RL + D + + I HE+
Sbjct: 1498 AWKKEYKLLHEYWMALRNRVSAVDELAMATERLRVRDPREPKPNPPVLHIIEPHEVEQNR 1557
Query: 1315 VQYSSDKFMSLAMLSRVKGKLRYLKGLVQSKQNIPSNNLRLAQEPAITSTSMEQKSENTS 1374
++ +DK ++ + L + G+L YL T++E+ + TS
Sbjct: 1558 IKLLNDKAVATSQLQKKLGQLLYL-------------------------TNLEKSQDKTS 1617
Query: 1375 KAYE-ESCPVCQEKLSNQKMVFQCGHMTCCKCLFAMTEK---TVHDSKIRTKWVMCPTCR 1434
E CP+C +L Q V CGH C +C+ + E+ H S I+ C CR
Sbjct: 1618 GGVNPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSSIK-----CAICR 1677
Query: 1435 QHTDFGNIAYVDDSKNETLDPSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTDS 1494
Q T I+YV S+ E I V+GS+ TK+EAVVR ++ I+ D
Sbjct: 1678 QTTSHKEISYVFTSE------------KANQEEDIPVKGSHSTKVEAVVRTLMKIQLRDP 1683
Query: 1495 KAKVLVFSSWNDVLDVLQYAFSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRAS 1554
AK LVFS+W DVLD++ A + N + F ++ + +S F+
Sbjct: 1738 GAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFK--------------- 1683
Query: 1555 AQVPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTF 1614
+ P++ +LLL + G+NGL ++EA HV+LVEP+LNPA E QAI RVHRIGQ T
Sbjct: 1798 -RDPQI---NILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTI 1683
Query: 1615 VHRFIVKDTVEESIHKLNRSRGSCSFITGNAKNQDQPFLTLKEVESLFVSKPSPLE 1654
VHRF++K T+EE + + ++ S +AK+ + LT+ ++ LF + LE
Sbjct: 1858 VHRFLIKATIEERMQAMLKT-AERSHTNSSAKHSEASVLTVADLADLFTKETEELE 1683
HSP 2 Score: 78.2 bits (191), Expect = 1.0e-12
Identity = 42/103 (40.78%), Postives = 60/103 (58.25%), Query Frame = 0
Query: 280 LDEDIPNLLPKLRPYQRRAAYWMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRMF 339
+D P L+P LRPYQR A WM+Q+E F + + + + E L +++
Sbjct: 293 VDVQHPALIPVLRPYQREAVNWMLQQE-CFRSSPATESALHFLWREIVTSEGL----KLY 352
Query: 340 YNPFSGNLTLNAEQILPHVYGGILADEMGLGKTIELLACILSH 383
YNP++G + P + GGILADEMGLGKT+E+LA IL+H
Sbjct: 353 YNPYTGCIIREYPNSGPQLLGGILADEMGLGKTVEVLALILTH 390
BLAST of Sed0014960.3 vs. ExPASy Swiss-Prot
Match:
Q7TPQ3 (E3 ubiquitin-protein ligase SHPRH OS=Mus musculus OX=10090 GN=Shprh PE=1 SV=1)
HSP 1 Score: 513.1 bits (1320), Expect = 1.2e-143
Identity = 380/1257 (30.23%), Postives = 572/1257 (45.51%), Query Frame = 0
Query: 415 RVECVCGAVSE-NHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRKEKRN 474
R EC+CG + H + VQC C WQHA C+ Y K +KP
Sbjct: 649 RFECICGEFDQIGHKPR---VQCLKCHLWQHAKCVNYEEKNLKVKPF------------- 708
Query: 475 KKNTVNITVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTCPDSM 534
C CL M+ P++T ATLI+ P+ I QW EI RH S+
Sbjct: 709 --------------YCPHCLVAME----PVSTRATLIISPSSICHQWVDEINRHVRSSSL 768
Query: 535 KLLVYEGVRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEG--DRRFMRFQ 594
++LVY+GV+ F +Q + L DIV+ +YDVL+ +L++ + H D R +R Q
Sbjct: 769 RVLVYQGVKKHGF-----LQPHFLAEQDIVIITYDVLRSELNYVNIPHSNSEDGRRLRNQ 828
Query: 595 KRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDL 654
KRY IP+PL + WWRICLDE QMVE A EMA RL +RWCI+GTP+QR LEDL
Sbjct: 829 KRYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGTPVQRGLEDL 888
Query: 655 YGLLRFVKASPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDELQLP 714
+GL+ F+ P+ V WW ++ PY +++P + ++F +IMWRS K V D++Q+P
Sbjct: 889 FGLVVFLGIEPYCVKHWWIRLLYHPYCKKNP---QHLYSFIAKIMWRSAKKDVIDQIQIP 948
Query: 715 PQVEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDVPSDLLI 774
PQ E++ WL FSP+E HFY RQHE C A ++ + D +K DR
Sbjct: 949 PQTEEMHWLHFSPVERHFYHRQHEVCCQDAIVKLRKISDWALKLSSLDR----------- 1008
Query: 775 THADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQYPMTMEEILMVLVSKTKIEGEEALRR 834
+L LL+LRQACCHPQ LQ+ MTMEE+L L K E EEA R+
Sbjct: 1009 --RTVSSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQ 1068
Query: 835 SVVALNALSGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLA 894
V ALN L+GI II+ +++ A LY+E L +EE+ + D L +H HNL E+L
Sbjct: 1069 LVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLG-- 1128
Query: 895 ADQSQSPLKNQTYSRSCEVKAYGMDTSENYDNHIMKKQKVSDTLYAPCGNTEKIEDNPLE 954
K + TL
Sbjct: 1129 ----------------------------------AKHPGIPPTL---------------- 1188
Query: 955 LTKKDTNTKKEENYELHQSSSYFNDNSLSQACETMRQKYLAVFSSKLSMAQQEFTKSYVQ 1014
D L + + +R+ Y++ +++++ AQQ + Q
Sbjct: 1189 -----------------------RDGRLEEEAKQLREHYMSKCNTEVAEAQQAL-QPVQQ 1248
Query: 1015 VCSELKERENLKEVWWLEAVQYAEQNKDFSHELIRKIEEAVSGNLNSSKSS-RVGSRFRS 1074
EL+ + + WWL + A EL++++ +S N + +FR
Sbjct: 1249 SIRELQRKIHSNSPWWLNVIHRA-MEFSVDEELVQRVRNEISSNYKQQTDKLSMSEKFRD 1308
Query: 1075 INALKYHVQTGLDLLEASRKVVLDRLLEIDQTMDNPKEEDIERVRYCQNCQADSNGPPCV 1134
L++ + T ++ L +K+V + + ++++ P E IE C A CV
Sbjct: 1309 CRGLQFLLTTQMEELHKFQKLVREAVKKLEKP---PSREVIESATVCHLRPARLPLNCCV 1368
Query: 1135 LCELDELFQEYEARLFRLNKVQGGMVTSVEEAVEAQK-----KKSALNRFYWSLLQQNKN 1194
C+ DELF EYE++LF N V+ G EE +E ++ + R W++
Sbjct: 1369 FCKADELFTEYESKLF-FNTVK-GQTAIFEEMIEDEEGLVDDRVPTTTRGLWAV------ 1428
Query: 1195 LSSSKVGNEESNKRDVGEKVMVSKHPSELEVVLGVIKNFCKT-QLGRESIGAANKQLHLL 1254
SE E + I +F ++ + E + + + L
Sbjct: 1429 --------------------------SETERSMKAILSFARSHRFDVEYVDEGSVSMDLF 1488
Query: 1255 EYMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLCLWDNDDDSS----AYAISEHELPAA 1314
E +KEY + ++A DE+ MAT RL + + + I HE+
Sbjct: 1489 EAWKKEYKLLHEYWMTLRNRVSAVDELAMATERLRVRHPKEPKPNPPVHHIIEPHEVEQN 1548
Query: 1315 SVQYSSDKFMSLAMLSRVKGKLRYLKGLVQSKQNIPSNNLRLAQEPAITSTSMEQKSENT 1374
++ +DK ++ + L + G+L YL T++E+ + T
Sbjct: 1549 RIKLVNDKAVATSQLQKKLGQLLYL-------------------------TNLEKSQDKT 1608
Query: 1375 SKAYE-ESCPVCQEKLSNQKMVFQCGHMTCCKCLFAMTEK---TVHDSKIRTKWVMCPTC 1434
S E CP+C +L Q V CGH C +C + E+ H S I+ C C
Sbjct: 1609 SGGINPEPCPICARQLGKQWAVLTCGHCFCNECTSIIIEQYSVGSHRSSIK-----CAIC 1668
Query: 1435 RQHTDFGNIAYVDDSKNETLDPSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTD 1494
RQ T ++YV S+ E I V+GS+ TK+EAVVR ++ I+ D
Sbjct: 1669 RQTTSHKEVSYVFTSE------------KANQEDDIPVKGSHSTKVEAVVRTLMKIQLRD 1674
Query: 1495 SKAKVLVFSSWNDVLDVLQYAFSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRA 1554
AK LVFS+W DVLD++ A + N + F ++ + +S F+
Sbjct: 1729 PGAKALVFSTWQDVLDIISKALTDNNMEFTQISRIKTFQENLSAFKYD------------ 1674
Query: 1555 SAQVPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKT 1614
P + +LLL + G+NGL ++EA HV+LVEP+LNPA E QAI RVHRIGQ T
Sbjct: 1789 ----PHI---NILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPT 1674
Query: 1615 FVHRFIVKDTVEESIHKLNRSRGSCSFITGNAKNQDQPFLTLKEVESLFVSKPSPLE 1654
VHRF++K T+EE + + ++ S + + K+ + LT+ + LF + LE
Sbjct: 1849 IVHRFLIKATIEERMQAMLKT-AERSHTSSSGKHSEASVLTVAGLADLFTKENEELE 1674
BLAST of Sed0014960.3 vs. ExPASy Swiss-Prot
Match:
Q9UTL9 (Uncharacterized ATP-dependent helicase C144.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC144.05 PE=3 SV=1)
HSP 1 Score: 222.2 bits (565), Expect = 4.3e-56
Identity = 322/1386 (23.23%), Postives = 561/1386 (40.48%), Query Frame = 0
Query: 285 PNLLPKLRPYQRRAAYWMVQR--EKGFSGNMGVGDKMQLISPLCMVLECLDTCSRMFYNP 344
P L +L P+Q R WM +R EK + N + PL + L ++ N
Sbjct: 212 PRLSSELLPFQMRVLEWMKRREEEKFLTSND--------LPPLWYHCKSLFDDRMVYVNH 271
Query: 345 FSGNLTLNAEQIL----PHVYGGILADEMGLGKTIELLACILSHRM-LAFEGAKGFDDEL 404
G +T + E+ + GGILADEMG+GKT+E+L +L H++ ++ FD
Sbjct: 272 VYGYMTFSKEKTYLLASGDIRGGILADEMGMGKTLEVLGLVLHHQLPISLTDTCTFD--- 331
Query: 405 QQFVGEQRPEFTRLKRERVECVCGAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRIL 464
Q VG+ + YS
Sbjct: 332 -QVVGKN-------------------------------------------VKYSK----- 391
Query: 465 KPIDIESGNSRKEKRNKKNTVNITVRDEEHVCMACLELMQATDAPMATGATLIVCPAPIL 524
ATLI+ P+ IL
Sbjct: 392 -------------------------------------------------ATLIITPSTIL 451
Query: 525 FQWRAEILRHTCPDSMKLLVYEGVRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHD 584
QW +EI H S+K+ Y+G+R ++ + + ++ DIV+TSY L+ +L +
Sbjct: 452 DQWLSEIDLHV--PSLKVFHYQGIRKSNGLKSAKI----FLDCDIVVTSYSDLRFELLYT 511
Query: 585 SDRHEGDRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRW 644
E R +R +KR+ +PL + WWRIC+DE QMVE++ + +M R+ + W
Sbjct: 512 ----ESHSRTLRHEKRHVSPKSPLIDVCWWRICVDEAQMVETSQSNVAQMIYRIPRVNCW 571
Query: 645 CITGTPIQRKLEDLYGLLRFVKASPFNVHR--WWAEVIRDPYERRDPGAMEFTHTFFKRI 704
++GTP++ +++DL+GLL ++ SP +++ W ++I R EF F +
Sbjct: 572 TVSGTPVRSEVDDLFGLLFLLRYSPMYLYKKQAWMQIIEKKRVR------EFC-DLFGSL 631
Query: 705 MWRSLKIHVTDELQLPPQVEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKR 764
+ R K V +EL+LPPQ S +EE YQ +S A + + KD +
Sbjct: 632 VCRHSKQDVEEELKLPPQHRICMTTRLSVVEETNYQ----DLLSEAAKSLHFFKDRNL-- 691
Query: 765 KVPDRVSSDVPSDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQYPM-TMEEIL 824
DL D + L++LRQACCHPQVG + PM ++ ++L
Sbjct: 692 ------------DL----CDEESMRRWLVRLRQACCHPQVGFGNKSAFGGGPMKSINDVL 751
Query: 825 MVLVSKTKIEGEEALRRSVVALNALSGIAIIEKKFSEAFSLYKEA---LELAEENNEDFR 884
+ ++ +T R+ + I K +++A +++ E +ELA + E+
Sbjct: 752 VFMLEQTNSTFSSLNRKLYSDKIIVGQIYDHIKDYNKALAIWSEVRIPVELAVKELENV- 811
Query: 885 LDPLLSIHIHHNLAEILPLAADQSQSPLKNQTYSRSCEVKAYGMDTSENYDNHIMKKQKV 944
I+++ K + + ++ + +G+D + + K+
Sbjct: 812 --------IYNS----------------KYEDHGKNLPINYFGLDHFIHLRVWYVLLHKI 871
Query: 945 ---SDTLYAPCGNTEKIEDNPLELTKKDTNTKKEENYELHQSSSYFNDNSLSQACETMRQ 1004
+ Y N EK E N L +D K + + S+ E +
Sbjct: 872 YFFIASAYFSLKN-EKFE-NEFYLLAQDLRRKIMSDVII----------KTSKHLEEFSE 931
Query: 1005 KYLAVFSSKLSMAQQEFTKSYVQVCSELKERENLKEVWWLEAVQYAEQNKDFSHELIR-- 1064
K++ K+ Q+ + K + +++ L Y E N D LI+
Sbjct: 932 KFIPKKLVKIPRLQKSYAKGLITGHGIIEDYNRL----------YKELN-DQKEVLIKFR 991
Query: 1065 -KIEEAVSGNLNSSKSSRVGSRF-RSINALKYHVQTGLD-LLEASRKVVLDRLLEIDQTM 1124
++ + L +S G + S+NA Q+ + ++ R+++ DR+ + T+
Sbjct: 992 DRLIHLMKLPLLDQESDPTGDEYEESLNA-----QSEISYCIDVYRQMLSDRVAAVSGTI 1051
Query: 1125 DN--PKEEDIERVRYCQNCQADSNGPPCVLCELDE----LFQEYEARLFRLNKVQGGMVT 1184
+ E ++E+ + ++ + E D+ F+E E + ++ G ++
Sbjct: 1052 NTFVSHETELEKYKLIESIKKSEKSLDKQAEERDKKYLLYFEEREEARPKADQ-YGSLIN 1111
Query: 1185 SVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSKVGNEESNKRDVGEKVMVSKHPSELEVV 1244
V ++A + + S + +KN+ EE + D K S+ +LE
Sbjct: 1112 IVSRLLDASNRST-------SSFETSKNM-------EEYERIDAMAKEQ-SRICQKLEKE 1171
Query: 1245 LGVIKNFCKTQLGRESIGAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEIKMATTR 1304
L +I QL S +EY ++ + SL ++ ++ +K +
Sbjct: 1172 LSII------QLTYNS---------RIEYYKQLQEISDSLMPPPVSNISLNNYVKDDEKK 1231
Query: 1305 LCLWDNDDDSSAY----AISEHELPAASVQYSSDKFMSLAMLSRVKGKLRYLKGLVQSKQ 1364
++ ++ ISE + A+ ++ + G + L+ L + K
Sbjct: 1232 QKFLNSVIIKASVILEKEISEKQDEASQTTNVAELVNQKISEMNIPGHIHLLRELEEEKS 1291
Query: 1365 NIPSNNLRLAQEPAITSTSMEQ---KSENTSKAYEESCPVCQEKLSNQKMVFQCGHMTCC 1424
N + E K+E+ + C +C++ + Q + CGH+ C
Sbjct: 1292 NTQRKIAHFESRRRYLTNLYEHIVLKAES-----HQICIICRD-IIKQGFITTCGHLYCS 1324
Query: 1425 KCLFAMTEKTVHDSKIRTKWVMCPTCRQHTDFGNIAYVDDSKN----------------- 1484
CL A + H S CP C+ + N Y+ +S++
Sbjct: 1352 FCLEAWLK---HSSS-------CPMCKTKLNKNNAYYIGESRDIYSRQEFVTGFNKRDER 1324
Query: 1485 -ETLDPSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLD 1544
E LD + S ++ ++ S+G+KI+ + + +L++K+ + KV+VFS W DVLD
Sbjct: 1412 LEILDDEAYRQIS-----NMELKESFGSKIDTISKHLLYLKHNELYPKVVVFSQWLDVLD 1324
Query: 1545 VLQYAFSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRASAQVPELRSAQVLLLL 1604
VL +F ANGI FIR G+ +T + F+ E RS QVL L
Sbjct: 1472 VLHKSFEANGIVFIRF-DGKSKNTCLKRFK-------------------EERSLQVLTLH 1324
Query: 1605 IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIH 1619
+ ++GL L A HV + EPLLN E QAISRVHRIGQ TFV+ +IV+DTVE I
Sbjct: 1532 ARSQSSGLTLTNATHVFMCEPLLNSGIEMQAISRVHRIGQTRPTFVYYYIVEDTVEGHIL 1324
BLAST of Sed0014960.3 vs. ExPASy Swiss-Prot
Match:
Q9FNI6 (DNA repair protein RAD5A OS=Arabidopsis thaliana OX=3702 GN=RAD5A PE=1 SV=1)
HSP 1 Score: 168.7 bits (426), Expect = 5.6e-40
Identity = 140/514 (27.24%), Postives = 213/514 (41.44%), Query Frame = 0
Query: 287 LLPKLRPYQRRAAYWMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRM--FYNPFS 346
LL +LRPYQ++A +WM Q EKG D+ + C CL + + N F+
Sbjct: 344 LLCELRPYQKQALHWMTQLEKG-----NCTDEAATMLHPCWEAYCLADKRELVVYLNSFT 403
Query: 347 GNLTLNAEQILPHVYGGILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQ 406
G+ T++ L GGILAD MGLGKT+ ++ +L+H A + GF
Sbjct: 404 GDATIHFPSTLQMARGGILADAMGLGKTVMTISLLLAHSWKA--ASTGF----------- 463
Query: 407 RPEFTRLKRERVECVCGAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIES 466
+C ++ I S ++
Sbjct: 464 ---------------------------------LCPNYEGDKVISSS----------VDD 523
Query: 467 GNSRKEKRNKKNTVNITVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEI 526
S K K + + +++ V + G LIVCP +L QW+ EI
Sbjct: 524 LTSPPVKATKFLGFDKRLLEQKSV--------------LQNGGNLIVCPMTLLGQWKTEI 583
Query: 527 LRHTCPDSMKLLVYEGVRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHD-SDRHEG 586
H P S+ + V+ G + LS SD+V+T+Y VL + S + S HEG
Sbjct: 584 EMHAKPGSLSVYVHYGQSRPKDAKLLS-------QSDVVITTYGVLTSEFSQENSADHEG 643
Query: 587 DRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTP 646
+ + W+RI LDE ++++ + + A+ L A RWC+TGTP
Sbjct: 644 -----------------IYAVRWFRIVLDEAHTIKNSKSQISLAAAALVADRRWCLTGTP 703
Query: 647 IQRKLEDLYGLLRFVKASPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIH 706
IQ LEDLY LLRF++ P+ WW ++++ P+E D ++ + K IM R K
Sbjct: 704 IQNNLEDLYSLLRFLRIEPWGTWAWWNKLVQKPFEEGDERGLKLVQSILKPIMLRRTKSS 736
Query: 707 VTDE----LQLPPQVEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPD 766
E L LPP +V + S E FY + R ++ D FV++
Sbjct: 764 TDREGRPILVLPPADARVIYCELSESERDFYD------ALFKRSKVK--FDQFVEQ---- 736
Query: 767 RVSSDVPSDLLITHADAGKLLSTLLKLRQACCHP 794
+L +A +L LL+LRQ C HP
Sbjct: 824 -------GKVLHNYA---SILELLLRLRQCCDHP 736
HSP 2 Score: 102.1 bits (253), Expect = 6.5e-20
Identity = 78/295 (26.44%), Postives = 135/295 (45.76%), Query Frame = 0
Query: 1353 MEQKSENTSKAYEESCPVCQEKLSNQKMVFQCGHMTCCKCLFAMTEKTVHDSKIRTKWVM 1412
+++ E K + CP+C E L + ++ C H C +CL A + +
Sbjct: 779 VQEVVEELRKGEQGECPICLEALED-AVLTPCAHRLCRECLLASWRNSTSG--------L 838
Query: 1413 CPTCRQHTDFGNIAYVDDSKNETLDPSTLHETSREHETSITVQGSY--GTKIEAVVRRIL 1472
CP CR SK E + T E + V+ ++ +KI A++ +
Sbjct: 839 CPVCRNTV----------SKQELITAPT------ESRFQVDVEKNWVESSKITALLEELE 898
Query: 1473 WIKYTDSKAKVLVFSSWNDVLDVLQYAFSANGITFIRMKGGRKSHTAISEFRGQEIDAKE 1532
++ + SK+ ++FS W LD+LQ S N +F+R+ G +
Sbjct: 899 GLRSSGSKS--ILFSQWTAFLDLLQIPLSRNNFSFVRLDG----------------TLSQ 958
Query: 1533 NTKKRASAQVPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRI 1592
+++ + E S VLL+ ++ G G+NL A + +++P NPA E QA+ R+HRI
Sbjct: 959 QQREKVLKEFSEDGSILVLLMSLKAGGVGINLTAASNAFVMDPWWNPAVEEQAVMRIHRI 1018
Query: 1593 GQENKTFVHRFIVKDTVEESIHKLNRSRGSCSFITGNAKNQDQPFLTLKEVESLF 1646
GQ + + RFIVK TVEE + + + I+G +Q+ ++E++ LF
Sbjct: 1019 GQTKEVKIRRFIVKGTVEERMEAVQARKQ--RMISGALTDQEVRSARIEELKMLF 1028
BLAST of Sed0014960.3 vs. ExPASy Swiss-Prot
Match:
Q9FIY7 (DNA repair protein RAD5B OS=Arabidopsis thaliana OX=3702 GN=RAD5B PE=3 SV=1)
HSP 1 Score: 165.6 bits (418), Expect = 4.8e-39
Identity = 145/521 (27.83%), Postives = 206/521 (39.54%), Query Frame = 0
Query: 291 LRPYQRRAAYWMVQREKGFSGNMGVGDKMQLISPLCMVLE-CLDTCSRMFYNPFSGNLTL 350
LRPYQ++A YWM + EKG + V + + P C + ++ N FSG T+
Sbjct: 616 LRPYQKQALYWMSESEKG----IDVEKAAETLHPCWEAYRICDERAPSIYLNIFSGEATI 675
Query: 351 NAEQILPHVYGGILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPEFT 410
GGILAD MGLGKT+ +A IL+ G PE
Sbjct: 676 QFPTATQMARGGILADAMGLGKTVMTIALILAR------------------PGRGNPENE 735
Query: 411 RLKRERVECVCGAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRK 470
D+ +
Sbjct: 736 ---------------------------------------------------DVLVADVNA 795
Query: 471 EKRNKKNTVNITVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTC 530
+KRN+K E+ A A G TLI+CP +L QW+ E+ H+
Sbjct: 796 DKRNRK------------------EIHMALTTVKAKGGTLIICPMALLSQWKDELETHSK 855
Query: 531 PDSMKLLVYEGVRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMR 590
PD++ +LVY G T + ++ + D+VLT+Y VL D
Sbjct: 856 PDTVSVLVYYGGDRTHDAKAIA-------SHDVVLTTYGVLTSAYKQD------------ 915
Query: 591 FQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLE 650
+ + RI W+RI LDE ++S T A + L + RWC+TGTP+Q KLE
Sbjct: 916 ------MANSIFHRIDWYRIVLDEAHTIKSWKTQAAKATFELSSHCRWCLTGTPLQNKLE 975
Query: 651 DLYGLLRFVKASPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDE-- 710
DLY LL F+ P+ WW+++I+ PYE DP ++ + +M R K E
Sbjct: 976 DLYSLLCFLHVEPWCNWAWWSKLIQKPYENGDPRGLKLIKAILRPLMLRRTKETRDKEGS 997
Query: 711 --LQLPPQVEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDV 770
L+LPP QV S E FY + R +Q D FV +
Sbjct: 1036 LILELPPTDVQVIECEQSEAERDFY------TALFKRSKVQ--FDQFVAQ---------- 997
Query: 771 PSDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQY 807
+L +A+ +L LL+LRQ C HP + S S QQY
Sbjct: 1096 -GKVLHNYAN---ILELLLRLRQCCNHPFLVMSRADS-QQY 997
HSP 2 Score: 98.2 bits (243), Expect = 9.4e-19
Identity = 80/313 (25.56%), Postives = 132/313 (42.17%), Query Frame = 0
Query: 1338 NNLRLAQEPAITSTSMEQKSENTSKAYEESCPVCQEKLSNQKMVFQCGHMTCCKCLFAMT 1397
NN + A + +E+ ++ + CP+C E ++ ++ C H C +CL
Sbjct: 1010 NNPDSVSQNAPSRAYIEEVIQDLRDGNSKECPICLES-ADDPVLTPCAHRMCRECLL--- 1069
Query: 1398 EKTVHDSKIRTKW-----VMCPTCRQHTDFGNIAYVDDSKNETLDPSTLHETSREHETSI 1457
T W +CP CR + E + T +
Sbjct: 1070 ----------TSWRSPSCGLCPICRTIL----------KRTELIS----CPTDSIFRVDV 1129
Query: 1458 TVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFSANGITFIRMKGGR 1517
+K+ +++ + IK + S K +VFS W LD+L+ G F+R G
Sbjct: 1130 VKNWKESSKVSELLKCLEKIKKSGSGEKSIVFSQWTSFLDLLEIPLRRRGFEFLRFDG-- 1189
Query: 1518 KSHTAISEFRGQEIDAKENTKKRASAQVPELRSAQVLLLLIQHGANGLNLLEAQHVVLVE 1577
+ +++ + E + +LL+ ++ G GLNL A V L++
Sbjct: 1190 --------------KLAQKGREKVLKEFNETKQKTILLMSLKAGGVGLNLTAASSVFLMD 1249
Query: 1578 PLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRGSCSFITGNAKNQD 1637
P NPA E QAI R+HRIGQ+ FV RFIVKDTVEE + ++ + I G +++
Sbjct: 1250 PWWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQVQARKQ--RMIAGALTDEE 1276
Query: 1638 QPFLTLKEVESLF 1646
L+E++ LF
Sbjct: 1310 VRSARLEELKMLF 1276
BLAST of Sed0014960.3 vs. ExPASy TrEMBL
Match:
A0A6J1BYG6 (E3 ubiquitin-protein ligase SHPRH OS=Momordica charantia OX=3673 GN=LOC111006415 PE=3 SV=1)
HSP 1 Score: 2901.7 bits (7521), Expect = 0.0e+00
Identity = 1464/1688 (86.73%), Postives = 1555/1688 (92.12%), Query Frame = 0
Query: 1 MGRRKQKKPHRSGGIRVEDHVNPKTQVDINGVVETVESSDIKLDVENEAFFVEVDRTGWN 60
MGRRKQ +PHRSGGIR+E H NP+TQVDI+ V+ET +SSDIKL+ NE F+VEVD TGW
Sbjct: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDISVVLETEKSSDIKLNEINEPFYVEVDPTGWK 60
Query: 61 SNEHHDISEVVLMDIRLEPHFVGFRLDESFCESFKYSLRFQLSNVNEFLLGRIKFGQWPV 120
SN+H DISEVVLMD+R E HFVGF+ D+SF ES +YSLRF+L NVNEFLLGRIKFG WP+
Sbjct: 61 SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
Query: 121 LSSSDISLELIERGIEEDKNACSVVLSGNFDGPDEAISGLVHLANLKFMTLRPVMGVTFS 180
LSSSDISLELI++G+EE +ACSVVLSGNFDGPD+AISGLVHLANLKFMTLRPVMG T S
Sbjct: 121 LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
Query: 181 HNIGSLRLRVELLSSAFDACESIFDSSRQLWKKSMMSTIAWLRPEVVSSEAKYGIAESTN 240
+ GSLRLRVE+LSSA DACESIFD+ RQLWKKSMMSTIAWLRPEV SSEAKYG+ +STN
Sbjct: 181 RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
Query: 241 MDSRLHHEMREDASDSRKHVNFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
+DS HEM + +S++RKH+NFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRMFYNPFSGNLTLNAEQILPHVYG 360
WMVQREK FSGN GVG+ +QLISPLC+ LECLDTC+RMFYNPFSGNLTL+AE+ LPHVYG
Sbjct: 301 WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPEFTRLKRERVECVC 420
GILADEMGLGKTIELLACILSHRM FEG KGF +E QQ VG QR EF RLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
Query: 421 GAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRKEKRNKKNTVNI 480
GAVSENH YKGLWVQCDICDAWQHADC+GYSPKGR+L+PID E GN K+KRNKKN VN+
Sbjct: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLRPIDTEGGNCIKKKRNKKNRVNV 480
Query: 481 TVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTCPDSMKLLVYEG 540
VRD +HVC+ CLELMQATD PMAT ATLIVCPAPILFQW AEILRHT P S+KLLVYEG
Sbjct: 481 VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
Query: 541 VRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VR TS SSTLSVQINDL+NSDI+LTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP
Sbjct: 541 VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDELQLPPQVEQVTWL 720
SPF+V+RWW EVIRDPYERRDPGAMEFTH FFK+IMWRSLKIHVTDELQLPPQ EQVTWL
Sbjct: 661 SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDVPSDLLITHADAGKLL 780
TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKV D VSSD PSD+LITH DAGKLL
Sbjct: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQYPMTMEEILMVLVSKTKIEGEEALRRSVVALNALS 840
STLLKLRQACCHPQVGSSGLRSLQQ PMTMEEILMVLVSKTKIEGEEALRRSVVALNAL+
Sbjct: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
Query: 841 GIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAADQSQSPLK 900
GIAIIEKKFSEAFSLYKEALELAE NNEDFRLDPLLSIHIHHNLAEILP A DQSQSPLK
Sbjct: 841 GIAIIEKKFSEAFSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
Query: 901 NQTYSRSCEVKAYGMDTSENYDNHIMKKQKVSDTLYAPCGNTEKIEDNPLELTKKDTNTK 960
+Q+YSR+CEVKA MD SENYDNH MKKQKVS+T YAPC +TEKI DNPL
Sbjct: 901 DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL---------- 960
Query: 961 KEENYELHQSSSYFNDNSLSQACETMRQKYLAVFSSKLSMAQQEFTKSYVQVCSELKERE 1020
+ H+SSSYFND+S +ACETMRQKYLAVFSSKLS+AQQEFTKS++QVCSEL+ERE
Sbjct: 961 -----KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCSELRERE 1020
Query: 1021 NLKEVWWLEAVQYAEQNKDFSHELIRKIEEAVSGNLNSSKSSRVGSRFRSINALKYHVQT 1080
+L +VWWLEAV +AEQNKDF HELIRKIEEAVSGN+N+S+SSRVGSRFRSINALKYHVQT
Sbjct: 1021 SLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQT 1080
Query: 1081 GLDLLEASRKVVLDRLLEIDQTMDNPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1140
GLDLLEASR VVLDRLLEIDQTM+NPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQE
Sbjct: 1081 GLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1140
Query: 1141 YEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSKVGNEESNKRD 1200
YEARLFRLNKVQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKNLSSS+VG EESNKRD
Sbjct: 1141 YEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRD 1200
Query: 1201 VGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIGAANKQLHLLEYMRKEYGHARSLAIA 1260
VGEKVMV KHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARSLAIA
Sbjct: 1201 VGEKVMVCKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIA 1260
Query: 1261 QAQVLNAHDEIKMATTRLCLWDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLSRVKG 1320
QAQVLNAHDEIKMATTRLCL DNDDD SAYAISEHELPAASVQYSSDKF+SLAMLSRVKG
Sbjct: 1261 QAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKG 1320
Query: 1321 KLRYLKGLVQSKQNIP---SNNLRLAQEPAITSTSMEQKSENTSKAYEESCPVCQEKLSN 1380
KLRYLKGLVQSKQN P S+NL L QEPAI STSMEQKSENT KA EESCPVCQEKLSN
Sbjct: 1321 KLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSN 1380
Query: 1381 QKMVFQCGHMTCCKCLFAMTEKTVHDSKIRTKWVMCPTCRQHTDFGNIAYVDDSKNETLD 1440
QKMVFQCGHM CCKCLFAMTEKT+ DSKI+T+WVMCPTCRQHTDFGNIA+ DDSKNE LD
Sbjct: 1381 QKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILD 1440
Query: 1441 PSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYA 1500
PSTLHETSREHET ITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYA
Sbjct: 1441 PSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYA 1500
Query: 1501 FSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRASAQVPELRSAQVLLLLIQHGA 1560
F+ANGITFIRMKGGRKS TAISEFRGQ+I+AKEN KKR AQVPEL+SAQVLLLLIQHGA
Sbjct: 1501 FAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQVLLLLIQHGA 1560
Query: 1561 NGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRS 1620
NGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRS
Sbjct: 1561 NGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRS 1620
Query: 1621 RGSCSFITGNAKNQDQPFLTLKEVESLFVSKPSPLEESDEKGSETLRHLAPSMAATIAAE 1680
R + SFITGNAKNQDQPFLTLKEVESLF SK SPL +SD+K SETLRHLAPSMAA +AAE
Sbjct: 1621 RENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAE 1671
Query: 1681 RRLRDHTA 1686
RRLR+HTA
Sbjct: 1681 RRLREHTA 1671
BLAST of Sed0014960.3 vs. ExPASy TrEMBL
Match:
A0A6J1FE16 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444885 PE=3 SV=1)
HSP 1 Score: 2899.8 bits (7516), Expect = 0.0e+00
Identity = 1461/1687 (86.60%), Postives = 1552/1687 (92.00%), Query Frame = 0
Query: 1 MGRRKQKKPHRSGGIRVEDHVNPKTQVDINGVVETVESSDIKLDVENEAFFVEVDRTGWN 60
MGRRKQK+PHRSGGIR ED V+PKTQVDING+VETVESSDIKLD NE FVEVDRTGW+
Sbjct: 1 MGRRKQKQPHRSGGIRFEDPVSPKTQVDINGIVETVESSDIKLDEVNEPLFVEVDRTGWH 60
Query: 61 SNEHHDISEVVLMDIRLEPHFVGFRLDESFCESFKYSLRFQLSNVNEFLLGRIKFGQWPV 120
SNEHHDISEVVLMDIRLE FVG + +E FCES +YSLRF+L N N FLL RIKFG WPV
Sbjct: 61 SNEHHDISEVVLMDIRLEHPFVGIQSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120
Query: 121 LSSSDISLELIERGIEEDKNACSVVLSGNFDGPDEAISGLVHLANLKFMTLRPVMGVTFS 180
LSS+DISLELIER +EED N CSVVLSG+FDGPDEAISGLVHLA+LKF+TLRPVMGV+FS
Sbjct: 121 LSSNDISLELIERRVEEDMNGCSVVLSGSFDGPDEAISGLVHLAHLKFVTLRPVMGVSFS 180
Query: 181 HNIGSLRLRVELLSSAFDACESIFDSSRQLWKKSMMSTIAWLRPEVVSSEAKYGIAESTN 240
N+GSLRLRVE+LSSAFDAC SI DS RQLWKKSMM+TIAWLRPEVVSSE KYG+ STN
Sbjct: 181 QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVGSTN 240
Query: 241 MDSRLHHEMREDASDSRKHVNFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
MD+ LH E +DAS SRKHVNFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 MDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRMFYNPFSGNLTLNAEQILPHVYG 360
WMVQREKGFS N GVG+ +QLISPLCM LECLDT SRMFYNPFSG+LT NAEQILPHVYG
Sbjct: 301 WMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQILPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPEFTRLKRERVECVC 420
GILADEMGLGKTIELLACILSHRM FE KG DDELQ VG+QR +F RLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHRMSVFESGKGLDDELQPLVGDQRTKFKRLKRERVECVC 420
Query: 421 GAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRKEKRNKKNTVNI 480
GAVSENH Y+GLWVQCDICDAWQHADC+GYSPKGRIL+P+D + GN RK+KRNK+NTVN+
Sbjct: 421 GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKRNKRNTVNV 480
Query: 481 TVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTCPDSMKLLVYEG 540
TVRDEEHVCM CLELMQATD+PM TGATLIVCPAPILFQW AEILRHT P S+KLLVYEG
Sbjct: 481 TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540
Query: 541 VRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VR TS S+T VQIND+INSDIVLTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541 VRDTSLSNTSPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDELQLPPQVEQVTWL 720
SPFNVHRWW EVIRDPYERRDPGAMEFTH FFK+IMWRSLK+HVTDELQLPPQ EQVTWL
Sbjct: 661 SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDVPSDLLITHADAGKLL 780
TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKVP VSSDVPSD+LITHADAGKLL
Sbjct: 721 TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQYPMTMEEILMVLVSKTKIEGEEALRRSVVALNALS 840
+TLLKLRQACCHPQVGSSGLRSLQQ PMTMEEILMVLVSKTKIEGEEALRR+VVALNAL+
Sbjct: 781 NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840
Query: 841 GIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAADQSQSPLK 900
GIAIIEKKFSEA SLYKEALELAEENNEDFR+DPLLSIHIHHNLAEILPLA DQ QSPLK
Sbjct: 841 GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900
Query: 901 NQTYSRSCEVKAYGMDTSENYDNHIMKKQKVSDTLYAPCGNTEKIEDNPLELTKKDTNTK 960
+Q Y R+CEVKA MD SE DNHI+KKQKV+ TLYAPC +TEKI D+PLELT D N K
Sbjct: 901 DQFYPRTCEVKASKMDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAK 960
Query: 961 KEENYELHQSSSYFNDNSLSQACETMRQKYLAVFSSKLSMAQQEFTKSYVQVCSELKERE 1020
KEENYE H+SSSYFNDN L +ACE MRQKYLAVFSSKL +AQQEFTKSYVQVC+ELK RE
Sbjct: 961 KEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVCNELKSRE 1020
Query: 1021 NLKEVWWLEAVQYAEQNKDFSHELIRKIEEAVSGNLNSSKSSRVGSRFRSINALKYHVQT 1080
NL EVWWL+AV +AEQNKDFSHELIRKIEEAVSGNLN+SK SRVGSRFRSI+ALKYHVQT
Sbjct: 1021 NLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKYHVQT 1080
Query: 1081 GLDLLEASRKVVLDRLLEIDQTMDNPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1140
GLDLLEASRK+V+ RLLEIDQTM++PK+EDIERVRYCQNCQADSNGPPCVLCELDELFQE
Sbjct: 1081 GLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1140
Query: 1141 YEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSKVGNEESNKRD 1200
YEARLFRLN VQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSS+ G EESNKRD
Sbjct: 1141 YEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEESNKRD 1200
Query: 1201 VGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIGAANKQLHLLEYMRKEYGHARSLAIA 1260
GE+V+VSKHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARSLAIA
Sbjct: 1201 AGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIA 1260
Query: 1261 QAQVLNAHDEIKMATTRLCLWDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLSRVKG 1320
QAQVLNAHDEIKMATTRLCL NDDD SAYAISEHELPAASVQYSS+KF+SLA LSRVKG
Sbjct: 1261 QAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSNKFVSLATLSRVKG 1320
Query: 1321 KLRYLKGLVQSKQNIP---SNNLRLAQEPAITSTSMEQKSENTSKAYEESCPVCQEKLSN 1380
KLRYLKGLVQSKQNIP S+N Q P ITSTS+EQKSENT KA EESCPVCQE LSN
Sbjct: 1321 KLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQETLSN 1380
Query: 1381 QKMVFQCGHMTCCKCLFAMTEKTVHDSKIRTKWVMCPTCRQHTDFGNIAYVDDSKNETLD 1440
QKMVFQCGHMTCCKCLFAMTEK + DS I+TKWVMCPTCRQHTDFGNIAY DDSKNETLD
Sbjct: 1381 QKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLD 1440
Query: 1441 PSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYA 1500
PSTLH+TSRE E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYA
Sbjct: 1441 PSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYA 1500
Query: 1501 FSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRASAQVPELRSAQVLLLLIQHGA 1560
F+AN ITF+RMKGGRKSHTAISEFRGQ+I+AKEN KK+ A+VPE+RSAQVLLLLIQHGA
Sbjct: 1501 FAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLIQHGA 1560
Query: 1561 NGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRS 1620
NGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH+LNRS
Sbjct: 1561 NGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHRLNRS 1620
Query: 1621 RGSCSFITGNAKNQDQPFLTLKEVESLFVSKPSPLEESDEKGSETLRHLAPSMAATIAAE 1680
R S SFITGN KNQDQP TLKEVESLF S+PSPL ESDEK SETLRHLAPSMAATIAAE
Sbjct: 1621 RESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAATIAAE 1680
Query: 1681 RRLRDHT 1685
RRL++HT
Sbjct: 1681 RRLKEHT 1686
BLAST of Sed0014960.3 vs. ExPASy TrEMBL
Match:
A0A6J1JXA7 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489159 PE=3 SV=1)
HSP 1 Score: 2883.2 bits (7473), Expect = 0.0e+00
Identity = 1453/1688 (86.08%), Postives = 1546/1688 (91.59%), Query Frame = 0
Query: 1 MGRRKQKKPHRSGGIRVEDHVNPKTQVDINGVVETVESSDIKLDVENEAFFVEVDRTGWN 60
MGRRKQK+PHRSGGIR ED VNPKT+VD NG+VETVESSDIKLD E FFVEVDRTGW+
Sbjct: 1 MGRRKQKQPHRSGGIRFEDRVNPKTEVDGNGIVETVESSDIKLDEGKEPFFVEVDRTGWH 60
Query: 61 SNEHHDISEVVLMDIRLEPHFVGFRLDESFCESFKYSLRFQLSNVNEFLLGRIKFGQWPV 120
SNEHHDISEVVLMDIRLE FVG R +E FCES +YSLRF+L N N FLL RIKFG WPV
Sbjct: 61 SNEHHDISEVVLMDIRLEHPFVGIRSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120
Query: 121 LSSSDISLELIERGIEEDKNACSVVLSGNFDGPDEAISGLVHLANLKFMTLRPVMGVTFS 180
LSS++ISLELIER IEED N CSVVLSG+FDGPDEAISGLVHLANLKF+TLRPVMGV+FS
Sbjct: 121 LSSNEISLELIERRIEEDMNGCSVVLSGSFDGPDEAISGLVHLANLKFVTLRPVMGVSFS 180
Query: 181 HNIGSLRLRVELLSSAFDACESIFDSSRQLWKKSMMSTIAWLRPEVVSSEAKYGIAESTN 240
N+GSLRLRVE+LSSAFDAC SI DS RQLWKKSMM+TIAWLRPEVVSSE KYG+ ESTN
Sbjct: 181 QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVESTN 240
Query: 241 MDSRLHHEMREDASDSRKHVNFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
M++ LH E +DAS SRKHVNFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 MEAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRMFYNPFSGNLTLNAEQILPHVYG 360
WMVQREKGFS N GVG+ QLISPLCM LECLDT RMFYNPFSG+LT NAEQ LPHVYG
Sbjct: 301 WMVQREKGFSRNFGVGENGQLISPLCMPLECLDTRLRMFYNPFSGDLTWNAEQTLPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPEFTRLKRERVECVC 420
GILADEMGLGKTIELLACILSH M F+G KGF+DE+QQ V +QR +F RLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHPMSVFKGGKGFEDEMQQLVEDQRTKFKRLKRERVECVC 420
Query: 421 GAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRKEKRNKKNTVNI 480
GAVSENH Y+GLWVQCDICDAWQHADC+GYSPKGRIL+P+D + GN RK+K NK+NTVN+
Sbjct: 421 GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKTNKRNTVNV 480
Query: 481 TVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTCPDSMKLLVYEG 540
TVRDEEHVCM CLELMQATD+PM TGATLIVCPAPILFQW AEILRHT P S+KLLVYEG
Sbjct: 481 TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540
Query: 541 VRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VR TS S+ L VQIND+INSDIVLTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541 VRDTSLSNPLPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDELQLPPQVEQVTWL 720
SPFNVHRWW EVIRDPYERRDPG MEFTH FFK+IMWRSLK+HVTDELQLPPQ EQVTWL
Sbjct: 661 SPFNVHRWWVEVIRDPYERRDPGTMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDVPSDLLITHADAGKLL 780
TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKVP VSSD PSD+LITHADAGKLL
Sbjct: 721 TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDAPSDILITHADAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQYPMTMEEILMVLVSKTKIEGEEALRRSVVALNALS 840
+TLLKLRQACCHPQVGSSGLRSLQQ PMTMEEILMVLVSKTKIEGEEALRR+VVALNAL+
Sbjct: 781 NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840
Query: 841 GIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAADQSQSPLK 900
GIAIIEKKFSEA SLYKEALELAEENNEDFR+DPLLSIHIHHNLAEILPLA DQ QSPLK
Sbjct: 841 GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900
Query: 901 NQTYSRSCEVKAYGMDTSENYDNHIMKKQKVSDTLYAPCGNTEKIEDNPLELTKKDTNTK 960
+Q Y R+CEVKA MD SE DNHI+KKQKV+ TLY PC +TEKI D+PLELT +D N K
Sbjct: 901 DQFYPRTCEVKASRMDDSEKSDNHIIKKQKVNGTLYVPCSDTEKIIDSPLELTGQDENAK 960
Query: 961 KEENYELHQSSSYFNDNSLSQACETMRQKYLAVFSSKLSMAQQEFTKSYVQVCSELKERE 1020
KEENYE H+SS+YFNDN L +ACE MRQKYLAVFSSKL +AQQEFTKSYVQVC+ELK RE
Sbjct: 961 KEENYEPHRSSNYFNDNYLRKACEDMRQKYLAVFSSKLCIAQQEFTKSYVQVCNELKSRE 1020
Query: 1021 NLKEVWWLEAVQYAEQNKDFSHELIRKIEEAVSGNLNSSKSSRVGSRFRSINALKYHVQT 1080
NL EVWWL+AV +AEQNKDFSHELIRKIEEAVSGNLN+SK SRVGSRFRSI+ALKYHVQT
Sbjct: 1021 NLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKYHVQT 1080
Query: 1081 GLDLLEASRKVVLDRLLEIDQTMDNPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1140
GLDLLEASRK+V+ RLLEIDQTM++PK+EDIERVRYCQNCQADSNGPPCVLCELD+LFQE
Sbjct: 1081 GLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDQLFQE 1140
Query: 1141 YEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSKVGNEESNKRD 1200
YEARLFRLN VQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSS+ G EESNKRD
Sbjct: 1141 YEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEESNKRD 1200
Query: 1201 VGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIGAANKQLHLLEYMRKEYGHARSLAIA 1260
GE+V+VSKHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARSLAIA
Sbjct: 1201 AGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIA 1260
Query: 1261 QAQVLNAHDEIKMATTRLCLWDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLSRVKG 1320
QAQVLNAHDEIKMATTRLCL ND D SAYAISEHELPAASVQYSSDKF+SLAMLSRVKG
Sbjct: 1261 QAQVLNAHDEIKMATTRLCLRGNDADDSAYAISEHELPAASVQYSSDKFVSLAMLSRVKG 1320
Query: 1321 KLRYLKGLVQSKQNIP---SNNLRLAQEPAITSTSMEQKSENTSKAYEESCPVCQEKLSN 1380
KLRYLKGLVQSKQNIP S+N Q P ITSTS+EQKSENT KA EESCPVCQE LSN
Sbjct: 1321 KLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQETLSN 1380
Query: 1381 QKMVFQCGHMTCCKCLFAMTEKTVHDSKIRTKWVMCPTCRQHTDFGNIAYVDDSKNETLD 1440
QKMVFQCGH+TCCKCLFAMTEK + DS I+TKWVMCPTCRQHTDFGNIAY DDSKN TLD
Sbjct: 1381 QKMVFQCGHITCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNATLD 1440
Query: 1441 PSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYA 1500
PSTLH+TSRE E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYA
Sbjct: 1441 PSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYA 1500
Query: 1501 FSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRASAQVPELRSAQVLLLLIQHGA 1560
F+AN ITF+RMKGGRKSHTAISEFRGQ+I+AKEN KK+ A+VPELRSAQVLLLLIQHGA
Sbjct: 1501 FAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPELRSAQVLLLLIQHGA 1560
Query: 1561 NGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRS 1620
NGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH LNRS
Sbjct: 1561 NGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHSLNRS 1620
Query: 1621 RGSCSFITGNAKNQDQPFLTLKEVESLFVSKPSPLEESDEKGSETLRHLAPSMAATIAAE 1680
R S SFITGN KNQDQP TLKEVESLF S+PSPL ESDEK SETLRHLAPSMAATIAAE
Sbjct: 1621 RESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAATIAAE 1680
Query: 1681 RRLRDHTA 1686
RRL++HTA
Sbjct: 1681 RRLKEHTA 1687
BLAST of Sed0014960.3 vs. ExPASy TrEMBL
Match:
A0A1S3C3N1 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496631 PE=3 SV=1)
HSP 1 Score: 2864.3 bits (7424), Expect = 0.0e+00
Identity = 1455/1692 (85.99%), Postives = 1554/1692 (91.84%), Query Frame = 0
Query: 1 MGRRKQKKPHRSGGIRVEDHVNPKTQVDINGVVET-VESSDIKLDVENEAFFVEVDRTGW 60
MGRRKQK+PH SGGIR ED +PKTQ+DINGV ET E+SDIKLD NE FVEVDR+GW
Sbjct: 1 MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60
Query: 61 NSNEHHDISEVVLMDIRLEPHFVGFRLDESFCESFKYSLRFQLSNVNEFLLGRIKFGQWP 120
SNEH+DISEV L+D+RLE FV F LD+S E+ +YSLRF+L NVN LL RIKFG WP
Sbjct: 61 YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120
Query: 121 VLSSSDISLELIERGIEEDKNACSVVLSGNFDGPDEAISGLVHLANLKFMTLRPVMGVTF 180
VLSS+DI LELIER IEED ACSVVLSGNFDGPDEAISGLVHLANLK MTLRPV GV+F
Sbjct: 121 VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180
Query: 181 SHNIGSLRLRVELLSSAFDACESIFDSSRQLWKKSMMSTIAWLRPEVVSSEAKYGIAEST 240
S N GSLRLRVE+LSSAFDACESIFD+ RQLWKKSMM+TIAWLRPEVV SE KYG+ +ST
Sbjct: 181 SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240
Query: 241 NMDSRLHHEMREDASDSRKHVNFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAA 300
NMD+ LHHE +DAS+SRKHVNFDT+ FYDAIKPSKD+P LDEDIPNLLPKLRPYQRRAA
Sbjct: 241 NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300
Query: 301 YWMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRMFYNPFSGNLTLNAEQILPHVY 360
YWMVQREKGFSGN G+G+ +QLISPLCM L+CL+TC R+FYNPFSGNLTLNAEQILPHVY
Sbjct: 301 YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360
Query: 361 GGILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPEFTRLKRERVECV 420
GGILADEMGLGKTIELLACILSH+M FEG KGFD E++Q V +QR EF RLKRERVEC+
Sbjct: 361 GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420
Query: 421 CGAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRKEKRNKKNTVN 480
CGAVSEN+ YKGLWVQCDICDAWQHADC+GYSPKGRI+KP+D E GNSR+EKRN KNT+N
Sbjct: 421 CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480
Query: 481 ITVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTCPDSMKLLVYE 540
+ VR EEHVC CLELMQATD+PMATGATLIVCPAPILFQW+AEILRHT P S+KLLVYE
Sbjct: 481 VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 540
Query: 541 GVRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
GVR TS TLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT
Sbjct: 541 GVRDTSLLGTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
Query: 601 PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601 PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660
Query: 661 ASPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDELQLPPQVEQVTW 720
ASPFNVH+WW EVIRDPYERRDPGAMEFTH FFK+IMWRSLKIHVTDELQLPPQ EQVTW
Sbjct: 661 ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
Query: 721 LTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDVPSDLLITHADAGKL 780
L FSP+EEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPD VSSD+PSDLL+THADAGKL
Sbjct: 721 LKFSPVEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVSSDIPSDLLVTHADAGKL 780
Query: 781 LSTLLKLRQACCHPQVGSSGLRSLQQYPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
LSTLLKLRQACCHPQVGSSGLRSLQQ PMTMEEILMVLVSKT+IEGEEALRRSVVALNAL
Sbjct: 781 LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840
Query: 841 SGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAADQSQSPL 900
+GIAII KKF EAFSLYKEALELAEEN EDFRLDPLLSIHIHHNLAEILPLA +QSQS L
Sbjct: 841 AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900
Query: 901 KNQTYSRSCEVKAYGMDTSENYDN--HIMKKQKVSDTLYAPCG--NTEKIEDNPLELTKK 960
K+Q R+CEVKA MD SE Y HIMKKQKVS+TLYA C NT K+ D+PL+LT K
Sbjct: 901 KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960
Query: 961 DTNTKKEENYELHQSSSYFNDNSLSQACETMRQKYLAVFSSKLSMAQQEFTKSYVQVCSE 1020
DTN KKEENYE H+S+SYF++ + + CE MRQKYLAVFSSKLS+AQQEFTKSY+QV SE
Sbjct: 961 DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020
Query: 1021 LKERENLKEVWWLEAVQYAEQNKDFSHELIRKIEEAVSGNLNSSKSSRVGSRFRSINALK 1080
LK+RENL +VWWLEAV +AEQNKDFSHELIRKIEEAVSGNLN+SK SRVGSRFRSI+ALK
Sbjct: 1021 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSISALK 1080
Query: 1081 YHVQTGLDLLEASRKVVLDRLLEIDQTMDNPKEEDIERVRYCQNCQADSNGPPCVLCELD 1140
YHVQ+GLDLLEASRKVVLDRLLEIDQTM+NPKEEDIERVRYC+NCQADSNGPPCVLCELD
Sbjct: 1081 YHVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELD 1140
Query: 1141 ELFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSKVGNEE 1200
ELFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQ+KN SSSKVG+EE
Sbjct: 1141 ELFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKVGHEE 1200
Query: 1201 SNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIGAANKQLHLLEYMRKEYGHAR 1260
NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESI AANKQLHLLEYMRKEYGHAR
Sbjct: 1201 PNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEYGHAR 1260
Query: 1261 SLAIAQAQVLNAHDEIKMATTRLCLWDNDDDSSAYAISEHELPAASVQYSSDKFMSLAML 1320
SLAIAQA VLNAHDEIKMATTRLCL DNDDDSSA++ISEHELPAASVQYSSDKFMSLAML
Sbjct: 1261 SLAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMSLAML 1320
Query: 1321 SRVKGKLRYLKGLVQSKQNIP---SNNLRLAQEPAITSTSMEQKSENTSKAYEESCPVCQ 1380
SRVKGKLRYLKGLVQSKQ IP S++L L QEPAITST+MEQK+ENT KA EESCP+CQ
Sbjct: 1321 SRVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESCPICQ 1380
Query: 1381 EKLSNQKMVFQCGHMTCCKCLFAMTEKTVHDSKIRTKWVMCPTCRQHTDFGNIAYVDDSK 1440
EKLSNQKMVFQCGH+TCCKCLFAMTEKT+H SKI+TKWVMCPTCRQHTDFGNIAY DDSK
Sbjct: 1381 EKLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSK 1440
Query: 1441 NETLDPSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLD 1500
NETLDPSTL ETSR+HE SITVQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLD
Sbjct: 1441 NETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLD 1500
Query: 1501 VLQYAFSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRASAQVPELRSAQVLLLL 1560
VL YAF+AN I+FIRMKGGRKS TAISEFRGQ+I+AKEN KKR S +PELRSAQVLLLL
Sbjct: 1501 VLHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLL 1560
Query: 1561 IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIH 1620
IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESI+
Sbjct: 1561 IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIY 1620
Query: 1621 KLNRSRGSCSFITGNAKNQDQPFLTLKEVESLFVSKPSPLEESDEKGSETLRHLAPSMAA 1680
KLNRSR S SFITGNAKNQDQPFLTLKEVESLF SK SPL E+D+KGSETLR LAPSMAA
Sbjct: 1621 KLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAA 1680
Query: 1681 TIAAERRLRDHT 1685
TIAAERRLR+HT
Sbjct: 1681 TIAAERRLREHT 1691
BLAST of Sed0014960.3 vs. ExPASy TrEMBL
Match:
A0A0A0K8S1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014630 PE=3 SV=1)
HSP 1 Score: 2851.2 bits (7390), Expect = 0.0e+00
Identity = 1451/1693 (85.71%), Postives = 1546/1693 (91.32%), Query Frame = 0
Query: 1 MGRRKQKKPHRSGGIRVEDHVNPKTQVDINGVVET-VESSDIKLDVENEAFFVEVDRTGW 60
MGRRKQK+P+RSGGIR ED + KTQ+DIN VVET VESSDIKLD NE FVEVDR+GW
Sbjct: 101 MGRRKQKQPNRSGGIRFEDRGDHKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 160
Query: 61 NSNEHHDISEVVLMDIRLEPHFVGFRLDESFCESFKYSLRFQLSNVNEFLLGRIKFGQWP 120
SNEH+DISEV L D+RLE FVGF LD+S E+ +YSLRF+L NVN LL RIKFG WP
Sbjct: 161 YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 220
Query: 121 VLSSSDISLELIERGIEEDKNACSVVLSGNFDGPDEAISGLVHLANLKFMTLRPVMGVTF 180
VLSS+D LE IER +EED ACSVVLSGN DGPDEAISGLVHLANLK MTLRPV GV F
Sbjct: 221 VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 280
Query: 181 SHNIGSLRLRVELLSSAFDACESIFDSSRQLWKKSMMSTIAWLRPEVVSSEAKYGIAEST 240
S N+GSLRLRVE+LSSAFDACESIFD+ RQLWKKSMM+TI WLRPEVV SE KYG+ +S+
Sbjct: 281 SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 340
Query: 241 NMDSRLHHEMREDASDSRKHVNFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAA 300
NMD+ LHHE +D S+SRKH NFDT+GFYDAIKPSKD+PMLDEDIPNLLPKLRPYQRRAA
Sbjct: 341 NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 400
Query: 301 YWMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRMFYNPFSGNLTLNAEQILPHVY 360
YWM+QREKGFSGN G+G+ +QLISPLCM L+CLDTC R+FYNPFSGNLTLNAEQ+LPHVY
Sbjct: 401 YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 460
Query: 361 GGILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPEFTRLKRERVECV 420
GGILADEMGLGKTIELLACILSH+M FEG KGFD E+QQ V +QR EF RLKRER+EC+
Sbjct: 461 GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 520
Query: 421 CGAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRKEKRNKKNTVN 480
CGAVSEN+ YKGLWVQCDICDAWQHADC+GYSPKGRI+K +D E GNSR+EKRN +NT+N
Sbjct: 521 CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 580
Query: 481 ITVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTCPDSMKLLVYE 540
+ VR EEHVC CLELMQATD+PMATGATLIVCPAPILFQW+AEILRHT P SMKLLVYE
Sbjct: 581 VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSMKLLVYE 640
Query: 541 GVRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
GVR TS TLS QINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT
Sbjct: 641 GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 700
Query: 601 PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK
Sbjct: 701 PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 760
Query: 661 ASPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDELQLPPQVEQVTW 720
ASPFNVHRWW EVIRDPYERRDPGAMEFTH FFK+IMWRSLKIHVTDELQLPPQ EQVTW
Sbjct: 761 ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 820
Query: 721 LTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDVPSDLLITHADAGKL 780
L FSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPD V+SD+PSDLLITHADAGKL
Sbjct: 821 LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 880
Query: 781 LSTLLKLRQACCHPQVGSSGLRSLQQYPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
LSTLLKLRQACCHPQVGSSGLRSLQQ PMTMEEILMVLVSKTKIEGEEALRRSVVALNAL
Sbjct: 881 LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 940
Query: 841 SGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAADQSQSPL 900
+GIAIIEKKFSEAFSLYKEALE AEENNEDFRLDPLLSIHIHHNLAEILPLA +QSQSPL
Sbjct: 941 AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 1000
Query: 901 KNQTYSRSCEVKAYGMDTSENYDN--HIMKKQKVSDTLYAPCG--NTEKIEDNPLELTKK 960
K+Q R+CEVKA MD SE YD+ HIMKKQKVS+TLYA C NT K+ D+PL+L K
Sbjct: 1001 KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 1060
Query: 961 DTNTKKEENYELHQSSSYFNDNSLSQACETMRQKYLAVFSSKLSMAQQEFTKSYVQVCSE 1020
DTN KKEENYE H+SS YF++ S+ + CE MRQKYLAVFSSKLS+AQQEFTKSY+QV SE
Sbjct: 1061 DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1120
Query: 1021 LKERENLKEVWWLEAVQYAEQNKDFSHELIRKIEEAVSGNLNSSKSSRVGSRFRSINALK 1080
LK+REN +VWWLEAV +AEQNKDFSHELIRKIEEAVSGNLN+SK SRVGSRFRSI+ALK
Sbjct: 1121 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSISALK 1180
Query: 1081 YHVQTGLDLLEASRKVVLDRLLEIDQTMDNPKEEDIERVRYCQNCQADSNGPPCVLCELD 1140
YHVQ+GLDLLEASRKVVLDRLLEIDQTM +PKEEDIERVRYC+ CQADSNGPPCVLCELD
Sbjct: 1181 YHVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELD 1240
Query: 1141 ELFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSKVGNEE 1200
ELFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKN SSSKVG+EE
Sbjct: 1241 ELFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEE 1300
Query: 1201 SNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIGAANKQLHLLEYMRKEYGHAR 1260
NKRD GEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLE+MRKEYGHAR
Sbjct: 1301 PNKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHAR 1360
Query: 1261 SLAIAQAQVLNAHDEIKMATTRLCL-WDNDDDSSAYAISEHELPAASVQYSSDKFMSLAM 1320
SLAIAQAQVLNAHDEIKMATTRL L D+DDDSSA+ ISEHELPAASVQYSSDKFMSLAM
Sbjct: 1361 SLAIAQAQVLNAHDEIKMATTRLSLRGDDDDDSSAFTISEHELPAASVQYSSDKFMSLAM 1420
Query: 1321 LSRVKGKLRYLKGLVQSKQNIP---SNNLRLAQEPAITSTSMEQKSENTSKAYEESCPVC 1380
LSRVKGKLRYLKGLVQSKQ IP S++L L QEPAITST+ EQK+ENTSKA EESCPVC
Sbjct: 1421 LSRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVC 1480
Query: 1381 QEKLSNQKMVFQCGHMTCCKCLFAMTEKTVHDSKIRTKWVMCPTCRQHTDFGNIAYVDDS 1440
QEKL+NQKMVFQCGH+TCCKCLFAMTEKT+H SKI+TKWVMCPTCRQHTDFGNIAY DDS
Sbjct: 1481 QEKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDS 1540
Query: 1441 KNETLDPSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVL 1500
KNETLDPST ETSREHE SITVQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVL
Sbjct: 1541 KNETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVL 1600
Query: 1501 DVLQYAFSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRASAQVPELRSAQVLLL 1560
DVL YAF AN ITFIRMKGGRKS TAISEFRGQ+I+AKEN KKR S +PELRSAQVLLL
Sbjct: 1601 DVLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLL 1660
Query: 1561 LIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESI 1620
LIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESI
Sbjct: 1661 LIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESI 1720
Query: 1621 HKLNRSRGSCSFITGNAKNQDQPFLTLKEVESLFVSKPSPLEESDEKGSETLRHLAPSMA 1680
+KLNRSR S SFITGNAKNQDQPFLTLKEVESLF SK SPL E+D+KGSETLR LAPSMA
Sbjct: 1721 YKLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMA 1780
Query: 1681 ATIAAERRLRDHT 1685
ATIAAERRLR+HT
Sbjct: 1781 ATIAAERRLREHT 1792
BLAST of Sed0014960.3 vs. TAIR 10
Match:
AT2G40770.1 (zinc ion binding;DNA binding;helicases;ATP binding;nucleic acid binding )
HSP 1 Score: 1736.9 bits (4497), Expect = 0.0e+00
Identity = 952/1709 (55.71%), Postives = 1206/1709 (70.57%), Query Frame = 0
Query: 1 MGRRKQKKPHRSGGIRVEDHVNPK-TQVDINGVV--ETVESSDIKLDVENEAFFVEVDRT 60
MGRRKQ KP R+ G+ + P ++ D N + E S D ++ ++ ++V + +
Sbjct: 1 MGRRKQSKPQRAVGL-----ITPTGSESDRNQLPGDEAEGSGDKNVEDIDKPYYVNICLS 60
Query: 61 GWNS--NEHHDISEVVLMDIRLEPHFVG----FRLDESFCESFKYSLRFQLSNVNEFLLG 120
S ++ D++EVVL + + VG F SLRF+L NV F +
Sbjct: 61 SRVSEQQQNFDLAEVVLTNFSVRER-VGSSSTFTKPIEVDHDLDCSLRFRLCNVTNF-VD 120
Query: 121 RIKFGQWPVLSSSDISLELIERGIEEDKNACSVVLSGNFDGPDEAISGLVHLANLKFMTL 180
RIK G WPVLSSSDI+LEL++ + +D+ SV+ S +FDGP E +SGL HLA++KF+TL
Sbjct: 121 RIKLGHWPVLSSSDITLELVDNKVSDDE-VGSVIWSASFDGPGEGVSGLAHLASIKFLTL 180
Query: 181 RPVMGVTFSHNIGSLRLRVELLSSAFDACESIFDSSRQLWKKSMMSTIAWLRPEVVSSEA 240
R + G + + S R+RVE+L AFDAC+S+ +++RQ+WKKSM+ ++WLRPEV++SEA
Sbjct: 181 RLMPG---NEGLLSPRVRVEMLQQAFDACDSLLENTRQIWKKSMIHVMSWLRPEVMTSEA 240
Query: 241 KYGIAESTNMDSRLHHEMREDASDSRKHVNFDTVGFYDAIKPSKDDPMLDEDIPNLLPKL 300
+YG + D ++ DS K +FD FY+AIKPSK D ML +DI +LLP+L
Sbjct: 241 RYGTRFNVK-DIESSVVTEDETLDSSKQSSFDAAAFYEAIKPSKTDAMLGDDIADLLPEL 300
Query: 301 RPYQRRAAYWMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRMFYNPFSGNLTLNA 360
RPYQRRAAYWMVQRE+G +G + Q ISPL + + LD+ ++MF NPFSGN++L
Sbjct: 301 RPYQRRAAYWMVQRERGDPITLGDKEDNQFISPLSISVGFLDSATKMFLNPFSGNISLTP 360
Query: 361 EQILPHVYGGILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPE---- 420
E P + GGILADEMGLGKT+ELLACI SHR K +DE+ G +
Sbjct: 361 EYFSPRIQGGILADEMGLGKTVELLACIFSHR-------KPAEDEISVSNGSSVTDVLNA 420
Query: 421 -FTRLKRERVECVCGAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGN 480
RLKRERVEC+CGAVSE+H YKG+WVQCD+CDAWQHADC+GYSPKG+ K
Sbjct: 421 GLRRLKRERVECICGAVSESHKYKGVWVQCDLCDAWQHADCVGYSPKGKGKKDSQ-HIDE 480
Query: 481 SRKEKRNKKNTVNITVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILR 540
+K++KK+ I VR+ E++C C EL+Q T +P++TGATLIVCPAPIL QW +EI R
Sbjct: 481 KASQKKSKKDATEIIVREGEYICQMCSELLQVTASPISTGATLIVCPAPILPQWHSEITR 540
Query: 541 HTCPDSMKLLVYEGVRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRR 600
HT S+ +YEGVR S S + I +L+N+DIVLT+YDVLKEDL+HD DRH+GDR
Sbjct: 541 HTRLGSLITCIYEGVRNASLSEEPMIDITELLNADIVLTTYDVLKEDLTHDFDRHDGDRH 600
Query: 601 FMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQR 660
+RFQKRYPVIPTPLTRIFWWRICLDE QMVESNA AATEMA RLY HRWCITGTPIQR
Sbjct: 601 CLRFQKRYPVIPTPLTRIFWWRICLDEAQMVESNAAAATEMALRLYTKHRWCITGTPIQR 660
Query: 661 KLEDLYGLLRFVKASPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTD 720
KL+DL+GLL+F+KA+PF+V RWW EVIRDPYERRD AMEFTH FFK++MWRS K+HV D
Sbjct: 661 KLDDLFGLLKFLKANPFDVSRWWIEVIRDPYERRDTKAMEFTHKFFKQVMWRSSKVHVAD 720
Query: 721 ELQLPPQVEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDVP 780
ELQLPPQ E V+WL FS IEEHFY RQH+TCVSYAREVI+ LK D +KR SSD P
Sbjct: 721 ELQLPPQEECVSWLKFSAIEEHFYSRQHDTCVSYAREVIETLKRDILKR---GHTSSDNP 780
Query: 781 SDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQYPMTMEEILMVLVSKTKIEGE 840
L+THA+A KLL++LLKLRQACCHPQVGSSGLRSLQQ PMTMEEILMVLV KT+ EGE
Sbjct: 781 ---LVTHAEAAKLLNSLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVKKTQSEGE 840
Query: 841 EALRRSVVALNALSGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAE 900
EALR +VALN ++ IA+++++FSEA SLYKEAL + EE+ EDFRLDPLL+IHI HNLAE
Sbjct: 841 EALRVLIVALNGIAAIAMLKQEFSEAVSLYKEALSITEEHAEDFRLDPLLNIHILHNLAE 900
Query: 901 ILPLAADQSQSPLKNQTYSRSCEVKAYGMDTSENYDNHIMKKQKVSDTLYAPCGNTEKIE 960
ILP+A +S + S E K +D ++ + K+Q++++ ++E +
Sbjct: 901 ILPMA----KSYGGKLSASGRPETK---IDVKDDDHHRASKRQRINELESLTHDSSETVH 960
Query: 961 DNPLELTKKDTNTKKEENYELHQSSSYFNDNSLSQACETMRQKYLAVFSSKLSMAQQEFT 1020
E D KK+ E H+ +L C+T++ KYL+ F+SKLS AQ EF
Sbjct: 961 QR--EAIAPDNGLKKDG--ECHEEC-----KTLDIVCDTLKVKYLSAFNSKLSAAQHEFK 1020
Query: 1021 KSYVQVCSELKERENLKEVWWLEAVQYAEQNKDFSHELIRKIEEAVSGNLNSSKSSRVGS 1080
KSY QV L + VWWL+A+Q EQNKDFS EL RKIEEA+ GNLN+S SSR S
Sbjct: 1021 KSYNQVSESLSNMGKQRSVWWLDALQLTEQNKDFSSELTRKIEEALHGNLNNSSSSRESS 1080
Query: 1081 RFRSINALKYHVQTGLDLLEASRKVVLDRLLEIDQTMDNPKEEDIERVRYCQNCQADSNG 1140
RFR+I+ +K H+QT +D+LE SRK V+DR+LEIDQTM+ PK EDIER+ C+ C +S+G
Sbjct: 1081 RFRTIHGMKLHLQTCMDMLERSRKKVIDRILEIDQTMEKPKLEDIERISNCKYCNKNSDG 1140
Query: 1141 PPCVLCELDELFQEYEARLFRLNKVQGGM--VTSVEEAVEAQKKKSALNRFYWSLLQQNK 1200
PPC+ CELDELFQEYEARLFRLNK + G+ + + EE V QKK+ A N F + L ++K
Sbjct: 1141 PPCIHCELDELFQEYEARLFRLNKSRRGVMEIAAAEETVHLQKKRDARNLFLFGLSSRSK 1200
Query: 1201 NLSSSKVGNEESNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIGAANKQLHLL 1260
+L++S+ +EE KR+ G+ V++SK PSE E+VLGVI+N CKT L RES AA K LH L
Sbjct: 1201 DLNASRGDDEEPTKRNAGDIVVLSKSPSETEIVLGVIRNHCKTHLDRESKLAATKHLHTL 1260
Query: 1261 EYMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLCLWDNDDDSSAYAISEHELPAASVQY 1320
E MRKEY HAR LA QAQ+L A+DEI M+T RL L +++DD+S YA+ EL ASV
Sbjct: 1261 EVMRKEYVHARVLARDQAQLLRAYDEINMSTMRLQLRESEDDTSIYALGRDELDVASVLN 1320
Query: 1321 SSDKFMSLAMLSRVKGKLRYLKGLVQSKQNIPSNNLRLA---QEPAITSTSMEQKSENTS 1380
++DKFM+ + L +KGKLRYLKGL++SKQ S + L+ E S EQ+SEN
Sbjct: 1321 TNDKFMAQSSLLSIKGKLRYLKGLMKSKQKQESESPDLSSPIHETVDASDPAEQESENLL 1380
Query: 1381 KAYEESCPVCQEKLSNQKMVFQCGHMTCCKCLFAMTEKTVHDSKIRTKWVMCPTCRQHTD 1440
K +E+CP+C E L NQKMVFQCGH TCC C FAMTE+ ++ KWVMCP CRQHTD
Sbjct: 1381 KR-DEACPICHEILRNQKMVFQCGHSTCCNCFFAMTERKSVQETLQ-KWVMCPICRQHTD 1440
Query: 1441 FGNIAYVDDSKNETLDPSTLHETSREHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKV 1500
NIAY DD +N S+ + ++ E S+ VQGSYGTKIEAV RRILWIK +D + KV
Sbjct: 1441 VRNIAYADDRRNS----SSSDQDHKDSEASLVVQGSYGTKIEAVTRRILWIKSSDPQTKV 1500
Query: 1501 LVFSSWNDVLDVLQYAFSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRASAQVP 1560
LVFSSWNDVLDVL++AF+AN IT IRMKGGRKS TAIS+F+G E + T+K S Q
Sbjct: 1501 LVFSSWNDVLDVLEHAFAANSITCIRMKGGRKSQTAISKFKGSE----KETQKTNSHQKE 1560
Query: 1561 ELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRF 1620
E +S QVLLLL+QHGANGLNLLEAQHV+LVEPLLNPAAEAQA+ RVHRIGQE T VHRF
Sbjct: 1561 E-KSIQVLLLLVQHGANGLNLLEAQHVILVEPLLNPAAEAQAVGRVHRIGQEKPTLVHRF 1620
Query: 1621 IVKDTVEESIHKLNRSRGS--CSFITGNAKNQDQPFLTLKEVESLFVS---KPSPLEESD 1680
+V TVEESI+KLNR++ + SF + N KNQDQ FLTLK++ESLF S + + +E++
Sbjct: 1621 LVSGTVEESIYKLNRNKNTNLSSFSSRNTKNQDQQFLTLKDLESLFASPTAETAEMEQNP 1656
Query: 1681 EKGSETLRHLAPSMAATIAAERRLRDHTA 1686
+ E LR L PS+AA +AAERR+++ A
Sbjct: 1681 GERQENLRDLPPSVAAALAAERRMKESNA 1656
BLAST of Sed0014960.3 vs. TAIR 10
Match:
AT5G22750.1 (DNA/RNA helicase protein )
HSP 1 Score: 168.7 bits (426), Expect = 4.0e-41
Identity = 140/514 (27.24%), Postives = 213/514 (41.44%), Query Frame = 0
Query: 287 LLPKLRPYQRRAAYWMVQREKGFSGNMGVGDKMQLISPLCMVLECLDTCSRM--FYNPFS 346
LL +LRPYQ++A +WM Q EKG D+ + C CL + + N F+
Sbjct: 344 LLCELRPYQKQALHWMTQLEKG-----NCTDEAATMLHPCWEAYCLADKRELVVYLNSFT 403
Query: 347 GNLTLNAEQILPHVYGGILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQ 406
G+ T++ L GGILAD MGLGKT+ ++ +L+H A + GF
Sbjct: 404 GDATIHFPSTLQMARGGILADAMGLGKTVMTISLLLAHSWKA--ASTGF----------- 463
Query: 407 RPEFTRLKRERVECVCGAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIES 466
+C ++ I S ++
Sbjct: 464 ---------------------------------LCPNYEGDKVISSS----------VDD 523
Query: 467 GNSRKEKRNKKNTVNITVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEI 526
S K K + + +++ V + G LIVCP +L QW+ EI
Sbjct: 524 LTSPPVKATKFLGFDKRLLEQKSV--------------LQNGGNLIVCPMTLLGQWKTEI 583
Query: 527 LRHTCPDSMKLLVYEGVRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHD-SDRHEG 586
H P S+ + V+ G + LS SD+V+T+Y VL + S + S HEG
Sbjct: 584 EMHAKPGSLSVYVHYGQSRPKDAKLLS-------QSDVVITTYGVLTSEFSQENSADHEG 643
Query: 587 DRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTP 646
+ + W+RI LDE ++++ + + A+ L A RWC+TGTP
Sbjct: 644 -----------------IYAVRWFRIVLDEAHTIKNSKSQISLAAAALVADRRWCLTGTP 703
Query: 647 IQRKLEDLYGLLRFVKASPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIH 706
IQ LEDLY LLRF++ P+ WW ++++ P+E D ++ + K IM R K
Sbjct: 704 IQNNLEDLYSLLRFLRIEPWGTWAWWNKLVQKPFEEGDERGLKLVQSILKPIMLRRTKSS 736
Query: 707 VTDE----LQLPPQVEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPD 766
E L LPP +V + S E FY + R ++ D FV++
Sbjct: 764 TDREGRPILVLPPADARVIYCELSESERDFYD------ALFKRSKVK--FDQFVEQ---- 736
Query: 767 RVSSDVPSDLLITHADAGKLLSTLLKLRQACCHP 794
+L +A +L LL+LRQ C HP
Sbjct: 824 -------GKVLHNYA---SILELLLRLRQCCDHP 736
HSP 2 Score: 102.1 bits (253), Expect = 4.6e-21
Identity = 78/295 (26.44%), Postives = 135/295 (45.76%), Query Frame = 0
Query: 1353 MEQKSENTSKAYEESCPVCQEKLSNQKMVFQCGHMTCCKCLFAMTEKTVHDSKIRTKWVM 1412
+++ E K + CP+C E L + ++ C H C +CL A + +
Sbjct: 779 VQEVVEELRKGEQGECPICLEALED-AVLTPCAHRLCRECLLASWRNSTSG--------L 838
Query: 1413 CPTCRQHTDFGNIAYVDDSKNETLDPSTLHETSREHETSITVQGSY--GTKIEAVVRRIL 1472
CP CR SK E + T E + V+ ++ +KI A++ +
Sbjct: 839 CPVCRNTV----------SKQELITAPT------ESRFQVDVEKNWVESSKITALLEELE 898
Query: 1473 WIKYTDSKAKVLVFSSWNDVLDVLQYAFSANGITFIRMKGGRKSHTAISEFRGQEIDAKE 1532
++ + SK+ ++FS W LD+LQ S N +F+R+ G +
Sbjct: 899 GLRSSGSKS--ILFSQWTAFLDLLQIPLSRNNFSFVRLDG----------------TLSQ 958
Query: 1533 NTKKRASAQVPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRI 1592
+++ + E S VLL+ ++ G G+NL A + +++P NPA E QA+ R+HRI
Sbjct: 959 QQREKVLKEFSEDGSILVLLMSLKAGGVGINLTAASNAFVMDPWWNPAVEEQAVMRIHRI 1018
Query: 1593 GQENKTFVHRFIVKDTVEESIHKLNRSRGSCSFITGNAKNQDQPFLTLKEVESLF 1646
GQ + + RFIVK TVEE + + + I+G +Q+ ++E++ LF
Sbjct: 1019 GQTKEVKIRRFIVKGTVEERMEAVQARKQ--RMISGALTDQEVRSARIEELKMLF 1028
BLAST of Sed0014960.3 vs. TAIR 10
Match:
AT5G43530.1 (Helicase protein with RING/U-box domain )
HSP 1 Score: 165.6 bits (418), Expect = 3.4e-40
Identity = 145/521 (27.83%), Postives = 206/521 (39.54%), Query Frame = 0
Query: 291 LRPYQRRAAYWMVQREKGFSGNMGVGDKMQLISPLCMVLE-CLDTCSRMFYNPFSGNLTL 350
LRPYQ++A YWM + EKG + V + + P C + ++ N FSG T+
Sbjct: 616 LRPYQKQALYWMSESEKG----IDVEKAAETLHPCWEAYRICDERAPSIYLNIFSGEATI 675
Query: 351 NAEQILPHVYGGILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPEFT 410
GGILAD MGLGKT+ +A IL+ G PE
Sbjct: 676 QFPTATQMARGGILADAMGLGKTVMTIALILAR------------------PGRGNPENE 735
Query: 411 RLKRERVECVCGAVSENHSYKGLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRK 470
D+ +
Sbjct: 736 ---------------------------------------------------DVLVADVNA 795
Query: 471 EKRNKKNTVNITVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTC 530
+KRN+K E+ A A G TLI+CP +L QW+ E+ H+
Sbjct: 796 DKRNRK------------------EIHMALTTVKAKGGTLIICPMALLSQWKDELETHSK 855
Query: 531 PDSMKLLVYEGVRGTSFSSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMR 590
PD++ +LVY G T + ++ + D+VLT+Y VL D
Sbjct: 856 PDTVSVLVYYGGDRTHDAKAIA-------SHDVVLTTYGVLTSAYKQD------------ 915
Query: 591 FQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLE 650
+ + RI W+RI LDE ++S T A + L + RWC+TGTP+Q KLE
Sbjct: 916 ------MANSIFHRIDWYRIVLDEAHTIKSWKTQAAKATFELSSHCRWCLTGTPLQNKLE 975
Query: 651 DLYGLLRFVKASPFNVHRWWAEVIRDPYERRDPGAMEFTHTFFKRIMWRSLKIHVTDE-- 710
DLY LL F+ P+ WW+++I+ PYE DP ++ + +M R K E
Sbjct: 976 DLYSLLCFLHVEPWCNWAWWSKLIQKPYENGDPRGLKLIKAILRPLMLRRTKETRDKEGS 997
Query: 711 --LQLPPQVEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDRVSSDV 770
L+LPP QV S E FY + R +Q D FV +
Sbjct: 1036 LILELPPTDVQVIECEQSEAERDFY------TALFKRSKVQ--FDQFVAQ---------- 997
Query: 771 PSDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQY 807
+L +A+ +L LL+LRQ C HP + S S QQY
Sbjct: 1096 -GKVLHNYAN---ILELLLRLRQCCNHPFLVMSRADS-QQY 997
HSP 2 Score: 98.2 bits (243), Expect = 6.6e-20
Identity = 80/313 (25.56%), Postives = 132/313 (42.17%), Query Frame = 0
Query: 1338 NNLRLAQEPAITSTSMEQKSENTSKAYEESCPVCQEKLSNQKMVFQCGHMTCCKCLFAMT 1397
NN + A + +E+ ++ + CP+C E ++ ++ C H C +CL
Sbjct: 1010 NNPDSVSQNAPSRAYIEEVIQDLRDGNSKECPICLES-ADDPVLTPCAHRMCRECLL--- 1069
Query: 1398 EKTVHDSKIRTKW-----VMCPTCRQHTDFGNIAYVDDSKNETLDPSTLHETSREHETSI 1457
T W +CP CR + E + T +
Sbjct: 1070 ----------TSWRSPSCGLCPICRTIL----------KRTELIS----CPTDSIFRVDV 1129
Query: 1458 TVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFSANGITFIRMKGGR 1517
+K+ +++ + IK + S K +VFS W LD+L+ G F+R G
Sbjct: 1130 VKNWKESSKVSELLKCLEKIKKSGSGEKSIVFSQWTSFLDLLEIPLRRRGFEFLRFDG-- 1189
Query: 1518 KSHTAISEFRGQEIDAKENTKKRASAQVPELRSAQVLLLLIQHGANGLNLLEAQHVVLVE 1577
+ +++ + E + +LL+ ++ G GLNL A V L++
Sbjct: 1190 --------------KLAQKGREKVLKEFNETKQKTILLMSLKAGGVGLNLTAASSVFLMD 1249
Query: 1578 PLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRGSCSFITGNAKNQD 1637
P NPA E QAI R+HRIGQ+ FV RFIVKDTVEE + ++ + I G +++
Sbjct: 1250 PWWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQVQARKQ--RMIAGALTDEE 1276
Query: 1638 QPFLTLKEVESLF 1646
L+E++ LF
Sbjct: 1310 VRSARLEELKMLF 1276
BLAST of Sed0014960.3 vs. TAIR 10
Match:
AT3G20010.1 (SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-related )
HSP 1 Score: 125.2 bits (313), Expect = 5.1e-28
Identity = 117/477 (24.53%), Postives = 191/477 (40.04%), Query Frame = 0
Query: 360 GGILADEMGLGKTIELLACILSHRMLAFEGAKGFDDELQQFVGEQRPEFTRLKRERVECV 419
GGILAD+ GLGKT+ +A IL Q+ V + + E + K+E V
Sbjct: 304 GGILADDQGLGKTVSTIALILK----------------QKIVSQLKSE-SSCKQETEALV 363
Query: 420 CGAVSENHSYK---GLWVQCDICDAWQHADCIGYSPKGRILKPIDIESGNSRKEKRNKKN 479
A E+ + K G V+ ++ + + + +L + +S ++ +
Sbjct: 364 LDADDESDNAKHESGSHVKPEL--------KVSSNSETSVLSACGNDENDSSDMEKAEDE 423
Query: 480 TVNITVRDEEHVCMACLELMQATDAPMATGATLIVCPAPILFQWRAEILRHTCPDS-MKL 539
N + R A TLIVCPA ++ QW E+ +S + +
Sbjct: 424 EANSSTR--------------AFQWKRPAAGTLIVCPASVVRQWARELDEKVSEESKLSV 483
Query: 540 LVYEGVRGTSFSSTLSVQINDLINSDIVLTSYDVL------------KEDLSHDSDRHEG 599
LVY G S + N+L D+V+T+Y ++ E+ ++DR+
Sbjct: 484 LVYHG-------SNRTKDPNELAEYDVVVTTYAIVTNEAPNKFLVDEDENDEKNTDRYGL 543
Query: 600 DRRFMRFQKRYPVIPT----------------------PLTRIFWWRICLDEGQMVESNA 659
F +KR V+ PL ++ W+RI LDE Q +++
Sbjct: 544 ASGFSNNKKRKVVVGASKKSKRRGRKSTNDTSSEPDCGPLGKVGWFRIVLDEAQTIKNYR 603
Query: 660 TAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKASPFNVHRWWAEVIRDPYERRD 719
T L A RWC++GTPIQ ++DLY RF++ P+ V++ + I+ P R
Sbjct: 604 TQMARSCCTLRAKRRWCLSGTPIQNTIDDLYSYFRFLRYDPYAVYKSFYSTIKVPISRNS 663
Query: 720 PGAMEFTHTFFKRIMWRSLKIHVTDE---LQLPPQVEQVTWLTFSPIEEHFYQRQHETCV 779
+ + IM R K + D + LPP+V ++ + FS E FY+
Sbjct: 664 CQGYKKLQAVLRAIMLRRTKGTLLDGKPIINLPPKVVNLSQVDFSVAERSFYK------- 712
Query: 780 SYAREVIQGLKDDFVKRKVPDRVSSDVPSDLLITHADAGKLLSTLLKLRQACCHPQV 796
K + R +D + +L LL+LRQAC HPQ+
Sbjct: 724 ---------------KLEADSRSQFKAYADAGTLSQNYANILLLLLRLRQACDHPQL 712
HSP 2 Score: 77.0 bits (188), Expect = 1.6e-13
Identity = 48/139 (34.53%), Postives = 74/139 (53.24%), Query Frame = 0
Query: 1480 KVLVFSSWNDVLDVLQYAFSANGITFIRMKGGRKSHTAISEFRGQEIDAKENTKKRASAQ 1539
K ++FS W +LD+++ +GI F R+ G ++ KE +KK
Sbjct: 894 KTIIFSQWTGMLDLVELRILESGIEFRRLDG-------TMSLAARDRAVKEFSKK----- 953
Query: 1540 VPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVH 1599
P+++ V+L+ ++ G GLN++ A HV+L++ NP E QAI R HRIGQ V
Sbjct: 954 -PDVK---VMLMSLKAGNLGLNMVAACHVILLDLWWNPTTEDQAIDRAHRIGQTRPVTVT 1013
Query: 1600 RFIVKDTVEESIHKLNRSR 1619
R +KDTVE+ I KL +
Sbjct: 1014 RITIKDTVEDRILKLQEEK 1016
BLAST of Sed0014960.3 vs. TAIR 10
Match:
AT1G11100.1 (SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-related )
HSP 1 Score: 120.6 bits (301), Expect = 1.3e-26
Identity = 83/295 (28.14%), Postives = 137/295 (46.44%), Query Frame = 0
Query: 508 TLIVCPAPILFQWRAEILRH-TCPDSMKLLVYEGVRGTSFSSTLSVQINDLINSDIVLTS 567
TLIVCP ++ QW E+ + T + +LVY G T ++L D+V+T+
Sbjct: 635 TLIVCPTSLMRQWADELRKKVTLEAHLSVLVYHGCSRTK-------DPHELAKYDVVITT 694
Query: 568 YDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATE 627
Y ++ + D + E + PL ++ W+R+ LDE Q +++ T A+
Sbjct: 695 YSLVSKRKHMDCEPVE-------------FLSGPLAQVSWFRVVLDEAQSIKNYKTQASI 754
Query: 628 MASRLYASHRWCITGTPIQRKLEDLYGLLRFVKASPFNVHRWWAEVIRDPYERRDPGAME 687
S L+A RWC++GTPIQ + DLY RF+K P++ ++ + E I++P +
Sbjct: 755 ACSGLHAKRRWCLSGTPIQNSIADLYSYFRFLKYDPYSSYQTFCETIKNPISSYPGEGYK 814
Query: 688 FTHTFFKRIMWRSLKIHVTD---ELQLPPQVEQVTWLTFSPIEEHFYQRQHETCVSYARE 747
K++M R K + D + LPP+ ++ + F+ E FY + C S
Sbjct: 815 TLQAILKKVMLRRTKDTLLDGKPVISLPPKSIELRRVDFTKEERDFYSKLE--CDS---- 874
Query: 748 VIQGLKDDFVKRKVPDRVSSDVPSDLLITHADAGKLLSTLLKLRQACCHPQVGSS 799
+D F + V + + LL+ LL+LRQAC HP + SS
Sbjct: 875 -----RDQFKEYAEAGTVKQNYVNILLM-----------LLRLRQACGHPLLVSS 887
HSP 2 Score: 73.9 bits (180), Expect = 1.3e-12
Identity = 51/173 (29.48%), Postives = 83/173 (47.98%), Query Frame = 0
Query: 1480 KVLVFSSWNDVLDVLQYAFSANGITFIRMKGGR---KSHTAISEFRGQEIDAKENTKKRA 1539
K +VF+ W +LD+L+ ++GI + R G A+ +F NT
Sbjct: 1073 KAIVFTQWTKMLDLLEAGLKSSGIQYRRFDGKMTVPARDAAVQDF---------NTLPDV 1132
Query: 1540 SAQVPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKT 1599
S + L++A + GLN++ A HV++++ NP E QAI R HRIGQ
Sbjct: 1133 SVMIMSLKAASL----------GLNMVAACHVIMLDLWWNPTTEDQAIDRAHRIGQTRPV 1192
Query: 1600 FVHRFIVKDTVEESIHKLNRSRGS--CSFITGNAKNQDQPFLTLKEVESLFVS 1648
V RF VKDTVE+ I L + + S + + L+++++ LF++
Sbjct: 1193 KVVRFTVKDTVEDRILALQQKKRKMVASAFGEHENGSRESHLSVEDLNYLFMA 1226
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_023551402.1 | 0.0e+00 | 86.84 | E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
XP_038884903.1 | 0.0e+00 | 87.40 | E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida] >XP_038884904.1... | [more] |
XP_022134046.1 | 0.0e+00 | 86.73 | E3 ubiquitin-protein ligase SHPRH [Momordica charantia] >XP_022134047.1 E3 ubiqu... | [more] |
XP_022938761.1 | 0.0e+00 | 86.60 | E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita moschata] | [more] |
KAG6578487.1 | 0.0e+00 | 86.43 | E3 ubiquitin-protein ligase SHPRH, partial [Cucurbita argyrosperma subsp. sorori... | [more] |
Match Name | E-value | Identity | Description | |
Q149N8 | 2.9e-145 | 30.25 | E3 ubiquitin-protein ligase SHPRH OS=Homo sapiens OX=9606 GN=SHPRH PE=1 SV=2 | [more] |
Q7TPQ3 | 1.2e-143 | 30.23 | E3 ubiquitin-protein ligase SHPRH OS=Mus musculus OX=10090 GN=Shprh PE=1 SV=1 | [more] |
Q9UTL9 | 4.3e-56 | 23.23 | Uncharacterized ATP-dependent helicase C144.05 OS=Schizosaccharomyces pombe (str... | [more] |
Q9FNI6 | 5.6e-40 | 27.24 | DNA repair protein RAD5A OS=Arabidopsis thaliana OX=3702 GN=RAD5A PE=1 SV=1 | [more] |
Q9FIY7 | 4.8e-39 | 27.83 | DNA repair protein RAD5B OS=Arabidopsis thaliana OX=3702 GN=RAD5B PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1BYG6 | 0.0e+00 | 86.73 | E3 ubiquitin-protein ligase SHPRH OS=Momordica charantia OX=3673 GN=LOC111006415... | [more] |
A0A6J1FE16 | 0.0e+00 | 86.60 | E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... | [more] |
A0A6J1JXA7 | 0.0e+00 | 86.08 | E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... | [more] |
A0A1S3C3N1 | 0.0e+00 | 85.99 | E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... | [more] |
A0A0A0K8S1 | 0.0e+00 | 85.71 | RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014630 P... | [more] |
Match Name | E-value | Identity | Description | |
AT2G40770.1 | 0.0e+00 | 55.71 | zinc ion binding;DNA binding;helicases;ATP binding;nucleic acid binding | [more] |
AT5G22750.1 | 4.0e-41 | 27.24 | DNA/RNA helicase protein | [more] |
AT5G43530.1 | 3.4e-40 | 27.83 | Helicase protein with RING/U-box domain | [more] |
AT3G20010.1 | 5.1e-28 | 24.53 | SNF2 domain-containing protein / helicase domain-containing protein / zinc finge... | [more] |
AT1G11100.1 | 1.3e-26 | 28.14 | SNF2 domain-containing protein / helicase domain-containing protein / zinc finge... | [more] |