Sed0011348.1 (mRNA) Chayote v1

Overview


Name	Sed0011348.1
Type	mRNA
Organism	Sechium edule (Chayote v1)
Description	Cytoplasmic tRNA 2-thiolation protein 1
Location	LG11: 26939112 .. 26945017 (-)
Sequence length	1687
RNA-Seq Expression	Sed0011348.1
Synteny	Sed0011348.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAGAATATATTATTATTGTAAATTCCAAAAAAAAAGAGAATTAAAAAAAATCAAATCAAATCGTTAGAAGAGAAGGTCGAGTTTCAGTTGGTATTTCTCCGCCGCCGCCGCGGCCTCGCCTTTTCAAACCTGAGGTCGAGCGATTCCGATTCCGATTTGAATTAGTGTCGGCGGCGTATTCGTGTATGGTTCAAAGTGGAACCGAGTTTTGGAAACCGTAGCGGAAGCAGTAAGGCATCCATGGAAGAAGGCGGTGCCAAACCAAGCAAGAAGGGCGTCCCTTGCATTTGCTGCATATGCAACAACCGGAAAGCTGCTCTCAAGAGGCCTAAAACCCTGCACCAGGTGTCTTCAATTTCCCCATATTCTTCTAATTCATCGTCTTAATTCTCGCAACCAATTTGAGACTCATCGGTCACCTAGGAATGGGCGTGGTTATCTGGTTATAGTTGAGTGAAGTTTCGATTTTCCAATTTAAAGAAAACCAAAAAAATTAGCATCTTAATTACTTACGTCCATTAATGTCGGCAAGGGGTGGCGCAGTGATTGAAAACTTGGGATTTGAGGGTATGCTCCCTTCAATGTTACAGCTTCGAGACTCACCCGTGACACTACCCTTTCGATGTCTCTGGTGCCTGGCCTAAGGATGGGCGTGGTTACCTTATTAAAAAAAAATTACTTAGGTCCATTAATATGTCATTGTGATTACCTATGACCTTCTAATACCCTTTTAAATTACCATTGAACTCAAAAGTTTCAAGCTTTGATATCCTACATTACCATGTCTATTGCTTTTACAGATATGCAGGGAATGTTTCTATGAGGTCTTTGAGGAGGAAATCCATCAGGTTATTGTGAAAAATCAGTTATTCAGACCTGGGGAACGCATTGCAGTTGGTGCATCTGGTGGAAAAGGTGGACACCGATTCTTCATAGTTCTAAATCTGATTATGTGAAAAAAATCTTATGGTATTTCTCTTGCCGCTTTATGCAGATTCTACCGTTCTTGCTTATGTGTTATCGAATTTGAATCGGCGCCACAATTATGGTTTGGATCTCTTTCTTTTATCAGTCGATGAGGGCATCACTGGATACAGAGATGACTCTCTTGAAACTGTGAAAAGAAATGAAATTCAGGTGATACATCCATTTGGACTTCTCTTAGATATATTCTCCCATTTTCTTTGCTTCTATAAATGTTTGTGCTTCACTATTACTATTAGTCTATTACTTTAATACATTGGGAATGGTAATATGATCATTTTGTGGGCGTAGACCCCTCCCCAGGTGGTAGTGAATCACTCTTAGTTCCCACTATTTCTTGATTGATTTATGCTTCTCTCTACGAGCTGTAAATTATAGAAACAAATCATTTGTACGAGGTTACATTTTTTCACCCCCATTCTCTTTTCAGTATGGGTTGCCACTACAAATTGTATCATACAAGGAATTATATGGATGGACAATGGATGAGATAGTGAAGATGATTGGTTTAAAGAATAATTGCACCTTTTGTGGAGTTTTCCGTCGACAGGTGTGAAACAACTCCTAATTTGCCTTTACAATTGTTGTCTGCTTTGCCCTCACAATTGTTGTCTGTGCATGGATTTCTTCCTTCTTTATATGAAATGGCTAACAAACCAATTGGTGTACAATCTTTCTGATCATAGTCTAGTTGTCATCTTATATGAACCTCTTGAACATGTGCAAGCGAAAGCAGTGCATTGAAGATAAATGTATAATGTATACTTCAGATATTGTCTGCAATTGCATTATGCATGTATTATATATGCTTTGCTGAGTTAGGAAAAAAGAGTTTCTACCATGGTTATTGCAAATGTCATTTGAAGCTTCCATTTATTTATTATCTTCTTTAGAGAGAAGCTTCCTTTTATTTAATGAAATAATGAAATAAAACGGTTTCTAATTTAGGATATGTTGTTGATGCTGCCCCTGAAAAAGAAAATAAAAAATAAAACGCCAAGGCAATGGTGGTATTGTAATCCCGAGCAGCGTGTGCTTGCTCATGAGTCAAGCACTTGCATGTCATGTACTACCGTAGTTTGTTTCCATTTTCTCGTTGTACATGAGTGGGACTCTATAGTTGTTGTTGCACTGTTAGTTAAGTACAGGCCATCCTTTCTTGACATAGGTTCTGTGTGCTTCTATAAGGCTTCGTGGTCTTTTTCAGTTCACTTGATCTTAAAAACCTCCCTTTCTATTGTTTGATTTTCTTGTGATCTATCACTAGGGGTATAGGTTGTTTTGTTGATAACATAACTGATGGTTCAATCGAATTTGTCAATTCAGTATGGCGTTTTGATGAATAATGTCGGGTCTATGGTTTTGTTAGGGTTGAGCCATAAAAGAAGGCATTCGGACTCACTAATCACTTGGTTTGTATATTCAAGCTTATAGGCTACTGATGTTTATTATTTAGTTGGTCAGTTTCATAATTTAATTTTATGAACCTCAAAGTGAAACCGACCAATGTTGGTTAATTTTTGTTGTAAGCCAAAAGTAAGCAACCGTAGGCCATTCTAACTAAGGTTGGCTGGATCAGTATTGTCCTTTAAATTTTCACCTTCAACTATCAACAATCATATTCTCATTTTGTTGTGGTATGTATTGAAAGATATATAGGATGTTGAGATGTAAGATCAGCCATAGAAAATCGATTTGGTAGACATTAGTTATTTGTATAATTGTATCTGTTCATTTCATGTATAAACATGTTTGAGTCACAATGAATGGACTAAGATGTGTATTAAGCATGATTTCTTTATATTGATGGATTTTTTTCCTCAAGGCTCTTGATAGAGGTGCTGCATTGTTGAAAGTCGACAAACTTGTCACTGGGCACAATGCAGATGACATGGCTGAAACAGTTCTATTAAATATACTACGAGGTGATATAGCAAGGTATTCTAATACTGAGAAGTCTTTTAAAATTAAAAAAATATTTTGTTCTCTCATTTGATCTGCATTAGATTGAACCAATGCTCTTGAATGTATAGATTGAGTAGATGTACTGCAATAATAACCGGTGAAGATGGACCGATTCCAAGATGCAAACCTTTCAAGTACGCATATGAAAAGGAAATCGTTATATATCCTTTTATACTTGTAGTCTCATAGTACTTGAGCAATCTTTGAATCAGCACCGTTCAGTTCAATTATTCCTTGTCTTGATACTACTTGTAAAACCTTAACCAGATATTCACGTATGCTTATTTTAAGAAGCTGGACTACTTCTCCACTGAGTGTAAGTCTTTATCTTTGTCATTCTTTGTTTTGTTTTTTTTTTCCTTCTGATTTCTCTGTCTACTAAGTACTAAATGGTAAAATACTAATTGATAATAAATACAGGTTCATTATATTTAATTTTTTTGCTACAAAATTGACGTTCTAATGGGCATTGTTTTAAAAGGCGCGTGCCTAGGCGCAAGGCGCACCTCTAGCGCCTCGCCCTGGAAAGGCGTGGCGCGTTGCCCCCGAGCCCCCATCGGGGGTCACGTTGCGCCCTTGAACCCCCAAAACCAGGCGCTTCGCCGCTAGACCCCGACTCGTTGACCGGGCAGCGAGACCCCCGTACTAGTCATTCGAAGCGCGACATAACTGGTTTAAGACATTTCAATACTTTAACTCACAAAGACAACATTCGCTTAAAATGAAAAGGTTAATAGTTGAATCTTATGTATGTTTTGTGTAGATTCTTAGTTAAACTTCATTTCTATCCATGGAATGAAGGTTAATAGTTAAATGTAATGTATGTTTTTTATATTATTCTTGGTTAAACTTCATTTATATCTATGGAAATGTTTTTATTGTGTATACACACATATATATAATTTTTTTGTGTTTTTTTTATATAGTGCGCCTTATGTAAAAAGTCCATGCCTTTTGGTGCGCTTGCGCCTCAGGCTCCAGAGACCTATTGTGCCTTAGTGCGCCCTGGGCTTTTAAAAACACTGCAAATGGGCTTAGTTAACTATGGAATTTGAAAGAAAGACTGAACAAGTGATCTGTTGAATTCATCAATTATAGGATCCAAAACTAGTTCTATCTATTACCGCCTTTTCATTGATCTAAATCATTTTCTTTTCTAGTGCTATCGTATCGTGTGGGGAGATATGTAACATTAATCATAATAACTCCATATTTTTCATCTTCAAACTTTGCATTTATGTCCTTATCCATTAAGAAATGTTGCATTCTTATTATGCATGGATCTCTAATGTTCTTTCTACAAAATGTTTTAAGTATCATCAAGCTTAGCATCACATAGTCTCTATTTGAAAATTTGACATTAATTTGTTCATCGATTGTTTGTTGGAATTGTAGGCATTTATTCTCCAAATGCTTATCGTGGTTTTGCTCGTGAATTTATCAAGGATTTGGAAAGAATCAGGTTTCAATTCACCTAGTCATTGTTTAACAAAAAATTATCCTTCTCGCTGCCAACAAGGATTGTGTACCCTCTGGTTTGCTCTCACTAAGCTTGTTGTATTATGAACACTAATGCAGACCTAGAGCCATTCTTGACATCATAAAATCAGGCGAGGATTTCAGGATTTCCAGTTCAACAAAAATGCCTGAGCAAGGAACATGTGAACGATGCGGCTACATTTCAAGCCAGGTATGTTTTGCTCTTATTTTACCGCGTAAGAGCACAAAATTTATAGCTCTAACAAGATTTTCTTAAACTGGTTTGAGCATATGTCATCATCTGAACTTACTGATCTTGAAGTATTTGTCGTCTTTTTGAAGATTACGTGTTCTGTTCAGAAACTCATCGACTGAATTCTGTGTACAAGAAATTGTAGCTACTCTGCATTATGCTTCTCCTACCCTTCATGTTTTAGCCTTTTTGGTAGTCTTTTCAATCTATAATTGGGTCCATCTGTGCTCCTCTCAACGTTTTAAGTTTGAAGCTTACCTGTGACATTAATTTGTAGACACTTTTTGTGTCTCCATTCTTTCAATGTCTCTCGGTGCTTGGTCTAGGAACAGATATGGTTAATCCCAAGTATAGTGGCGAAGCTCCGATTTCCTGGTTTAGAAATAATTGGGTCAATCTTGAAGGCAAGTTATTATTTTTTGACAACCTACTTTCAATGACATATCGAATGTTGTCGATTTTTCAAGTACCTACCTATATCTCACTGATATTTTAGGCATGGCTATTATTCCTTTTTCTATATTTCTGTTTCTTTCACAAGTGCCTTGAAATATGGCTTGCACCTATTTCATTTACATCTACATGAGAGCTCTCTCACTGTTCATTTTGAAATTCATTTCTAATTTTCTGGAATTTGACTTGCATACAATTAAATTAAGACTCTGATCATTCCATTTACTAATGCAGAAATGGTGTAAAGCTTGTGTTCTGCTCGAGGGCCTCAACCGAGGTTTACCGAAGTTGGGTATTGGTCGGAGTCGAGTTCTTGACAATGAGGGGAAGAGAGAAATGAAGGGAAGTAGTAATGGACATACTAGAAGCATTGAGAGCAAACAGTGTGGCACTCTAGACTTCTGACGCTTTTCTGTATTCATAACAGTGCTTCAATGGTGATCAAAATATCAGATGAAAGTTACAACATTCTTCGCTGTTTCGGCTGGATGATCTCGATTTCTGTTTTAGATGGAAGATGAAAGAGTGAATGCAAATGAAGAAAAAGAAATAGATCAGCATATCTGATTTGACACCAGCAATACAAACAATGAAGTCCATTGAGAAACTTTGGGCATCCATGTAGCAATATTTTTTTCTCCAGAATTCTCATTGTATAACATAAATTTTATATTTATTTTCTTCAATGTTAGAACTTCTAGTTTTTTAAGTCCAAATTTCAAAATAGATGCTTTGATAGTTGACCAAAAGGATTCTTTTCTTGAAATAATT

mRNA sequence

GCAGAATATATTATTATTGTAAATTCCAAAAAAAAAGAGAATTAAAAAAAATCAAATCAAATCGTTAGAAGAGAAGGTCGAGTTTCAGTTGGTATTTCTCCGCCGCCGCCGCGGCCTCGCCTTTTCAAACCTGAGGTCGAGCGATTCCGATTCCGATTTGAATTAGTGTCGGCGGCGTATTCGTGTATGGTTCAAAGTGGAACCGAGTTTTGGAAACCGTAGCGGAAGCAGTAAGGCATCCATGGAAGAAGGCGGTGCCAAACCAAGCAAGAAGGGCGTCCCTTGCATTTGCTGCATATGCAACAACCGGAAAGCTGCTCTCAAGAGGCCTAAAACCCTGCACCAGATATGCAGGGAATGTTTCTATGAGGTCTTTGAGGAGGAAATCCATCAGGTTATTGTGAAAAATCAGTTATTCAGACCTGGGGAACGCATTGCAGTTGGTGCATCTGGTGGAAAAGATTCTACCGTTCTTGCTTATGTGTTATCGAATTTGAATCGGCGCCACAATTATGGTTTGGATCTCTTTCTTTTATCAGTCGATGAGGGCATCACTGGATACAGAGATGACTCTCTTGAAACTGTGAAAAGAAATGAAATTCAGTATGGGTTGCCACTACAAATTGTATCATACAAGGAATTATATGGATGGACAATGGATGAGATAGTGAAGATGATTGGTTTAAAGAATAATTGCACCTTTTGTGGAGTTTTCCGTCGACAGGCTCTTGATAGAGGTGCTGCATTGTTGAAAGTCGACAAACTTGTCACTGGGCACAATGCAGATGACATGGCTGAAACAGTTCTATTAAATATACTACGAGGTGATATAGCAAGATTGAGTAGATGTACTGCAATAATAACCGGTGAAGATGGACCGATTCCAAGATGCAAACCTTTCAAGTACGCATATGAAAAGGAAATCGTTATGTATGCTTATTTTAAGAAGCTGGACTACTTCTCCACTGAGTGCATTTATTCTCCAAATGCTTATCGTGGTTTTGCTCGTGAATTTATCAAGGATTTGGAAAGAATCAGACCTAGAGCCATTCTTGACATCATAAAATCAGGCGAGGATTTCAGGATTTCCAGTTCAACAAAAATGCCTGAGCAAGGAACATGTGAACGATGCGGCTACATTTCAAGCCAGAAATGGTGTAAAGCTTGTGTTCTGCTCGAGGGCCTCAACCGAGGTTTACCGAAGTTGGGTATTGGTCGGAGTCGAGTTCTTGACAATGAGGGGAAGAGAGAAATGAAGGGAAGTAGTAATGGACATACTAGAAGCATTGAGAGCAAACAGTGTGGCACTCTAGACTTCTGACGCTTTTCTGTATTCATAACAGTGCTTCAATGGTGATCAAAATATCAGATGAAAGTTACAACATTCTTCGCTGTTTCGGCTGGATGATCTCGATTTCTGTTTTAGATGGAAGATGAAAGAGTGAATGCAAATGAAGAAAAAGAAATAGATCAGCATATCTGATTTGACACCAGCAATACAAACAATGAAGTCCATTGAGAAACTTTGGGCATCCATGTAGCAATATTTTTTTCTCCAGAATTCTCATTGTATAACATAAATTTTATATTTATTTTCTTCAATGTTAGAACTTCTAGTTTTTTAAGTCCAAATTTCAAAATAGATGCTTTGATAGTTGACCAAAAGGATTCTTTTCTTGAAATAATT

Coding sequence (CDS)

ATGGAAGAAGGCGGTGCCAAACCAAGCAAGAAGGGCGTCCCTTGCATTTGCTGCATATGCAACAACCGGAAAGCTGCTCTCAAGAGGCCTAAAACCCTGCACCAGATATGCAGGGAATGTTTCTATGAGGTCTTTGAGGAGGAAATCCATCAGGTTATTGTGAAAAATCAGTTATTCAGACCTGGGGAACGCATTGCAGTTGGTGCATCTGGTGGAAAAGATTCTACCGTTCTTGCTTATGTGTTATCGAATTTGAATCGGCGCCACAATTATGGTTTGGATCTCTTTCTTTTATCAGTCGATGAGGGCATCACTGGATACAGAGATGACTCTCTTGAAACTGTGAAAAGAAATGAAATTCAGTATGGGTTGCCACTACAAATTGTATCATACAAGGAATTATATGGATGGACAATGGATGAGATAGTGAAGATGATTGGTTTAAAGAATAATTGCACCTTTTGTGGAGTTTTCCGTCGACAGGCTCTTGATAGAGGTGCTGCATTGTTGAAAGTCGACAAACTTGTCACTGGGCACAATGCAGATGACATGGCTGAAACAGTTCTATTAAATATACTACGAGGTGATATAGCAAGATTGAGTAGATGTACTGCAATAATAACCGGTGAAGATGGACCGATTCCAAGATGCAAACCTTTCAAGTACGCATATGAAAAGGAAATCGTTATGTATGCTTATTTTAAGAAGCTGGACTACTTCTCCACTGAGTGCATTTATTCTCCAAATGCTTATCGTGGTTTTGCTCGTGAATTTATCAAGGATTTGGAAAGAATCAGACCTAGAGCCATTCTTGACATCATAAAATCAGGCGAGGATTTCAGGATTTCCAGTTCAACAAAAATGCCTGAGCAAGGAACATGTGAACGATGCGGCTACATTTCAAGCCAGAAATGGTGTAAAGCTTGTGTTCTGCTCGAGGGCCTCAACCGAGGTTTACCGAAGTTGGGTATTGGTCGGAGTCGAGTTCTTGACAATGAGGGGAAGAGAGAAATGAAGGGAAGTAGTAATGGACATACTAGAAGCATTGAGAGCAAACAGTGTGGCACTCTAGACTTCTGA

Protein sequence

MEEGGAKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFRPGERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHNADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPKLGIGRSRVLDNEGKREMKGSSNGHTRSIESKQCGTLDF

Homology

[Close]

BLAST of Sed0011348.1 vs. NCBI nr
Match: XP_023002400.1 (cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita maxima])

HSP 1 Score: 691.4 bits (1783), Expect = 4.1e-195
Identity = 342/361 (94.74%), Postives = 348/361 (96.40%), Query Frame = 0


Query: 1   MEEGGAKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFR 60
           MEE GAKP +KGV C+CCIC+NRKAALKRPKTL QICRECFYEVFEEEIHQVIVKN LFR
Sbjct: 1   MEEAGAKP-RKGVSCLCCICDNRKAALKRPKTLQQICRECFYEVFEEEIHQVIVKNHLFR 60

Query: 61  PGERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120
           PGERIAVGASGGKDSTVLAYVLS LNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI
Sbjct: 61  PGERIAVGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120

Query: 121 QYGLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHN 180
           QYGLPLQIVSYK+LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKL TGHN
Sbjct: 121 QYGLPLQIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHN 180

Query: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYF 240
           ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKY YEKEIVMYAYFKKLDYF
Sbjct: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYF 240

Query: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYI 300
           STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIS+STKMPEQGTCERCGYI
Sbjct: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISTSTKMPEQGTCERCGYI 300

Query: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRVLDNE-GKREMKG-SSNGHTRSIESKQCGTLD 360
           SSQKWCKACVLLEGLNRGLPKLGIGRSR LDNE  KREMKG SS+G TRSIESKQCGTLD
Sbjct: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRGLDNETEKREMKGRSSDGQTRSIESKQCGTLD 360

[Close]

BLAST of Sed0011348.1 vs. NCBI nr
Match: XP_038886415.1 (cytoplasmic tRNA 2-thiolation protein 1 [Benincasa hispida])

HSP 1 Score: 689.9 bits (1779), Expect = 1.2e-194
Identity = 337/359 (93.87%), Postives = 343/359 (95.54%), Query Frame = 0


Query: 1   MEEGGAKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFR 60
           MEE G KP KK V  +CC+CNNRKAALKRPKTL QICRECFYEVFEEEIHQVIVKNQLFR
Sbjct: 1   MEEAGPKP-KKAVSSLCCLCNNRKAALKRPKTLQQICRECFYEVFEEEIHQVIVKNQLFR 60

Query: 61  PGERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120
           PGERIAVGASGGKDSTVLAYVLS LN+RHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI
Sbjct: 61  PGERIAVGASGGKDSTVLAYVLSELNQRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120

Query: 121 QYGLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHN 180
           QYGLPLQIVSYK+LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKL TGHN
Sbjct: 121 QYGLPLQIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHN 180

Query: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYF 240
           ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKY YEKEIVMYAYFKKLDYF
Sbjct: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYF 240

Query: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYI 300
           STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIS+STKMPEQG CERCGYI
Sbjct: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISTSTKMPEQGMCERCGYI 300

Query: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRVLDNEGKREMKGSSNGHTRSIESKQCGTLDF 360
           SSQKWCKACVLLEGLNRGLPKLGIGRSR  D+EGKRE  GSSNGH RSIESKQCGTLDF
Sbjct: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRGHDSEGKRETNGSSNGHKRSIESKQCGTLDF 358

[Close]

BLAST of Sed0011348.1 vs. NCBI nr
Match: XP_023538431.1 (cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita pepo subsp. pepo] >XP_023538432.1 cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 689.5 bits (1778), Expect = 1.5e-194
Identity = 342/361 (94.74%), Postives = 347/361 (96.12%), Query Frame = 0


Query: 1   MEEGGAKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFR 60
           MEE GAKP +KGV C+C ICNNRKAALKRPKTL QICRECFYEVFEEEIHQVIVKN LFR
Sbjct: 1   MEEAGAKP-RKGVSCLCGICNNRKAALKRPKTLQQICRECFYEVFEEEIHQVIVKNHLFR 60

Query: 61  PGERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120
           PGERIAVGASGGKDSTVLAYVLS LNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI
Sbjct: 61  PGERIAVGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120

Query: 121 QYGLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHN 180
           QYGLPLQIVSYK+LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKL TGHN
Sbjct: 121 QYGLPLQIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHN 180

Query: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYF 240
           ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKY YEKEIVMYAYFKKLDYF
Sbjct: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYF 240

Query: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYI 300
           STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIS+STKMPEQGTCERCGYI
Sbjct: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISTSTKMPEQGTCERCGYI 300

Query: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRVLDNEG-KREMKG-SSNGHTRSIESKQCGTLD 360
           SSQKWCKACVLLEGLNRGLPKLGIGRSR LDNE  KREMKG SS+G TRSIESKQCGTLD
Sbjct: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRGLDNEAEKREMKGRSSDGQTRSIESKQCGTLD 360

[Close]

BLAST of Sed0011348.1 vs. NCBI nr
Match: KAG6585514.1 (Cytoplasmic tRNA 2-thiolation protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 686.8 bits (1771), Expect = 1.0e-193
Identity = 341/361 (94.46%), Postives = 347/361 (96.12%), Query Frame = 0


Query: 1   MEEGGAKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFR 60
           MEE GAK S+KGV C+C ICNNRKAALKRPKTL QICRECFYEVFEEEIHQVIVKN LFR
Sbjct: 1   MEEAGAK-SRKGVSCLCGICNNRKAALKRPKTLQQICRECFYEVFEEEIHQVIVKNHLFR 60

Query: 61  PGERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120
           PGERIAVGASGGKDSTVLAYVLS LNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI
Sbjct: 61  PGERIAVGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120

Query: 121 QYGLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHN 180
           QYGLPLQIVSYK+LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKL TGHN
Sbjct: 121 QYGLPLQIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHN 180

Query: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYF 240
           ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKY YEKEIVMYAYFKKLDYF
Sbjct: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYF 240

Query: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYI 300
           STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIS+STKMPEQGTCERCGYI
Sbjct: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISTSTKMPEQGTCERCGYI 300

Query: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRVLDNE-GKREMKG-SSNGHTRSIESKQCGTLD 360
           SSQKWCKACVLLEGLNRGLPK+GIGRSR LDNE  KREMKG SS+G TRSIESKQCGTLD
Sbjct: 301 SSQKWCKACVLLEGLNRGLPKMGIGRSRGLDNETEKREMKGRSSDGQTRSIESKQCGTLD 360

[Close]

BLAST of Sed0011348.1 vs. NCBI nr
Match: XP_022951723.1 (cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita moschata])

HSP 1 Score: 686.4 bits (1770), Expect = 1.3e-193
Identity = 341/361 (94.46%), Postives = 347/361 (96.12%), Query Frame = 0


Query: 1   MEEGGAKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFR 60
           MEE GAK S++GV C+C ICNNRKAALKRPKTL QICRECFYEVFEEEIHQVIVKN LFR
Sbjct: 1   MEEAGAK-SRRGVSCLCGICNNRKAALKRPKTLQQICRECFYEVFEEEIHQVIVKNHLFR 60

Query: 61  PGERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120
           PGERIAVGASGGKDSTVLAYVLS LNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI
Sbjct: 61  PGERIAVGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120

Query: 121 QYGLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHN 180
           QYGLPLQIVSYK+LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKL TGHN
Sbjct: 121 QYGLPLQIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHN 180

Query: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYF 240
           ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKY YEKEIVMYAYFKKLDYF
Sbjct: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYF 240

Query: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYI 300
           STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIS+STKMPEQGTCERCGYI
Sbjct: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISTSTKMPEQGTCERCGYI 300

Query: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRVLDNE-GKREMKG-SSNGHTRSIESKQCGTLD 360
           SSQKWCKACVLLEGLNRGLPKLGIGRSR LDNE  KREMKG SS+G TRSIESKQCGTLD
Sbjct: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRGLDNETEKREMKGRSSDGQTRSIESKQCGTLD 360

[Close]

BLAST of Sed0011348.1 vs. ExPASy Swiss-Prot
Match: O64862 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Arabidopsis thaliana OX=3702 GN=NCS6 PE=2 SV=2)

HSP 1 Score: 626.7 bits (1615), Expect = 1.6e-178
Identity = 301/354 (85.03%), Postives = 326/354 (92.09%), Query Frame = 0


Query: 6   AKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFRPGERI 65
           AK  K     +CC+CN R+  LKRPKTL QICRECFYEVFEEEIHQVIV+N+LF+ GER+
Sbjct: 3   AKNKKAVASRLCCLCNLRRPVLKRPKTLQQICRECFYEVFEEEIHQVIVQNRLFKSGERV 62

Query: 66  AVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLP 125
           A+GASGGKDSTVLAYVLS LNRRHNYGLDLFLLS+DEGITGYRDDSLETVKRNE+QYGLP
Sbjct: 63  AIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSIDEGITGYRDDSLETVKRNEVQYGLP 122

Query: 126 LQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHNADDMA 185
           L+IVSYK+LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKV+KLVTGHNADD+A
Sbjct: 123 LKIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDIA 182

Query: 186 ETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYFSTECI 245
           ETVLLNILRGDIARLSRCT+I TGEDGPIPRCKPFKY YEKEIVMYAYFKKLDYFSTECI
Sbjct: 183 ETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTECI 242

Query: 246 YSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYISSQKW 305
           YSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRI+++TKMPEQGTCERCGYISSQKW
Sbjct: 243 YSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIATTTKMPEQGTCERCGYISSQKW 302

Query: 306 CKACVLLEGLNRGLPKLGIGRSRVLDNEGKREMKGSSNGHTRSIESKQCGTLDF 360
           CKACVLLEGLNRGLPK+GIGR R ++ +  +E K       +SIESKQCG+LDF
Sbjct: 303 CKACVLLEGLNRGLPKMGIGRPRGVNGDHNKETK-KPGSVAKSIESKQCGSLDF 355

[Close]

BLAST of Sed0011348.1 vs. ExPASy Swiss-Prot
Match: Q6Z6G6 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCS6 PE=2 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 8.3e-167
Identity = 286/358 (79.89%), Postives = 314/358 (87.71%), Query Frame = 0


Query: 3   EGGAKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFRPG 62
           +G  +P  +    +C  C  RKAALKRPKTL QICRECFY VFE+EIHQ IV N LF+PG
Sbjct: 6   DGPRQPPARAGSRLCTRCGERKAALKRPKTLEQICRECFYVVFEDEIHQTIVDNNLFKPG 65

Query: 63  ERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQY 122
           +R+A+GASGGKDSTVLAYVLS LNRRH Y LDLFLLSVDEGITGYRDDSLETVKRNEIQY
Sbjct: 66  DRVAIGASGGKDSTVLAYVLSELNRRHKYCLDLFLLSVDEGITGYRDDSLETVKRNEIQY 125

Query: 123 GLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHNAD 182
           GLPL+IVSYK+LYGWTMD+IVK IGLKNNCTFCGVFRRQALDRGAALLKVDK+VTGHNAD
Sbjct: 126 GLPLKIVSYKDLYGWTMDDIVKAIGLKNNCTFCGVFRRQALDRGAALLKVDKIVTGHNAD 185

Query: 183 DMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYFST 242
           D+AETVLLNILRGDIARLSRCT I TGEDGPIPRCKPFKY YEKEIVMYAYFKKLDYFST
Sbjct: 186 DIAETVLLNILRGDIARLSRCTFITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFST 245

Query: 243 ECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYISS 302
           ECIYSPNAYRGFAREFIKDLER+RPRAILDIIKSGE+FRIS++T+MPEQGTCERCGYISS
Sbjct: 246 ECIYSPNAYRGFAREFIKDLERMRPRAILDIIKSGENFRISTTTRMPEQGTCERCGYISS 305

Query: 303 QKWCKACVLLEGLNRGLPKLGIGRSR-VLDNEGKREMKGSSNGHTRSIESKQCGTLDF 360
           QK CKACVLL+GLNRGLPKLGIGR++ +   +G  E + + +   RS    + G  DF
Sbjct: 306 QKLCKACVLLDGLNRGLPKLGIGRTKGIAGGDGDCEQQATRSERNRSSLQGKHGNFDF 363

[Close]

BLAST of Sed0011348.1 vs. ExPASy Swiss-Prot
Match: A8JF71 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Chlamydomonas reinhardtii OX=3055 GN=NCS6 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 3.3e-139
Identity = 233/317 (73.50%), Postives = 268/317 (84.54%), Query Frame = 0


Query: 14  PCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFRPGERIAVGASGGK 73
           P +C  CN  +A L RPKT  Q+CRECF+  FEEE+HQ IV N+LF+ GER+AV ASGGK
Sbjct: 3   PRLCVRCNTSRAMLNRPKTQEQVCRECFFTAFEEEVHQTIVDNKLFKHGERLAVAASGGK 62

Query: 74  DSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLQIVSYKE 133
           DSTVLA+++S LN R+NYGLDLFLLS+DEGITGYRDDSLETVKRNE QYG+PL++VSYKE
Sbjct: 63  DSTVLAHIMSTLNARYNYGLDLFLLSIDEGITGYRDDSLETVKRNEQQYGIPLKVVSYKE 122

Query: 134 LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHNADDMAETVLLNIL 193
           LYGWTMDEIV  IG KNNCTFCGVFRRQALDRGA L+  +K+ TGHNADD+AETVLLNIL
Sbjct: 123 LYGWTMDEIVSQIGTKNNCTFCGVFRRQALDRGAVLMGANKIATGHNADDVAETVLLNIL 182

Query: 194 RGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYFSTECIYSPNAYRG 253
           RGD+ RL RC  IITGED  +PR KPFKYAYEKEIV+YAY+KKLDYFSTECIY+P A RG
Sbjct: 183 RGDVPRLGRCANIITGEDSSLPRVKPFKYAYEKEIVLYAYYKKLDYFSTECIYAPFAARG 242

Query: 254 FAREFIKDLERIRPRAILDIIKSGED--FRISSSTKMPEQGTCERCGYISSQKWCKACVL 313
           FAREFIKDLE  RP AI+D+I+S ED  F  +S  K+P+  TCERCGYISSQ  CKACVL
Sbjct: 243 FAREFIKDLEAARPLAIIDVIRSAEDFVFAAASDEKLPQPRTCERCGYISSQPVCKACVL 302

Query: 314 LEGLNRGLPKLGIGRSR 329
           LEGLNRG+P+LG+ R+R
Sbjct: 303 LEGLNRGMPRLGVSRTR 319

[Close]

BLAST of Sed0011348.1 vs. ExPASy Swiss-Prot
Match: Q5FW05 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus tropicalis OX=8364 GN=ctu1 PE=2 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 4.3e-139
Identity = 231/321 (71.96%), Postives = 268/321 (83.49%), Query Frame = 0


Query: 13  VPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFRPGERIAVGASGG 72
           +P  C  C  R+A L+RPKT H +C++CF+  FEEEIH  IV  +LF PGE++ +GASGG
Sbjct: 1   MPVNCSSCEERRAVLRRPKTGHSLCKDCFFHAFEEEIHHTIVSAKLFNPGEKVGIGASGG 60

Query: 73  KDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLQIVSYK 132
           KDSTVLA+VL  LN R+ YGLDL L+SVDEGI+GYRDDSLETVKRN+ QY LPL+IVSY+
Sbjct: 61  KDSTVLAHVLKVLNERYAYGLDLILVSVDEGISGYRDDSLETVKRNQQQYELPLKIVSYQ 120

Query: 133 ELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHNADDMAETVLLNI 192
           ELYGWTMD+IVK +GLKNNCTFCGVFRRQALDRGA +L V+K+ TGHNADD+AETVL+N 
Sbjct: 121 ELYGWTMDQIVKQVGLKNNCTFCGVFRRQALDRGAMMLGVNKICTGHNADDIAETVLMNF 180

Query: 193 LRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYFSTECIYSPNAYR 252
           LRGDIARL RCT+I TG +G IPRCKP KYAYEKEIV+YAYFKKLDYFSTECIYSPNAYR
Sbjct: 181 LRGDIARLRRCTSITTGSEGAIPRCKPLKYAYEKEIVLYAYFKKLDYFSTECIYSPNAYR 240

Query: 253 GFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYISSQKWCKACVLL 312
           G AR F+KDLE IRP +I+DII SGE+  +    +MP QGTC RCGYISSQ  CKACVLL
Sbjct: 241 GHARAFLKDLEAIRPSSIMDIIHSGENLSVKEDVRMPVQGTCTRCGYISSQSLCKACVLL 300

Query: 313 EGLNRGLPKLGIGRSRVLDNE 334
           EGLNRGLPKLGIG+   L ++
Sbjct: 301 EGLNRGLPKLGIGKHHKLHHK 321

[Close]

BLAST of Sed0011348.1 vs. ExPASy Swiss-Prot
Match: Q05AW7 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus laevis OX=8355 GN=ctu1 PE=2 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 4.0e-137
Identity = 228/321 (71.03%), Postives = 266/321 (82.87%), Query Frame = 0


Query: 13  VPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFRPGERIAVGASGG 72
           +P  C  C   +A L+RPKT H +C++CF+  FEEEIH  IV  +LF PGE++ +GASGG
Sbjct: 1   MPVKCSSCEEWRAVLRRPKTGHSLCKDCFFYAFEEEIHNTIVSAKLFHPGEKVGIGASGG 60

Query: 73  KDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLQIVSYK 132
           KDSTVLA+V+  LN R+ YGLDL LLSVDEGI+GYRDDSL+TVKRN+ QY LPL+IVSY 
Sbjct: 61  KDSTVLAHVMKVLNERYAYGLDLILLSVDEGISGYRDDSLQTVKRNQQQYELPLKIVSYL 120

Query: 133 ELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHNADDMAETVLLNI 192
           ELYGWTMD+IVK +GLKNNCTFCGVFRRQALDRGA +L ++K+ TGHNADD+AETVL+N 
Sbjct: 121 ELYGWTMDQIVKQVGLKNNCTFCGVFRRQALDRGAMMLGINKICTGHNADDIAETVLMNF 180

Query: 193 LRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYFSTECIYSPNAYR 252
           LRGDIARL RCTAI TG +G IPRCKP KY YEKEIV+YAYFKKLDYFSTECIYSPNAYR
Sbjct: 181 LRGDIARLRRCTAITTGSEGAIPRCKPLKYTYEKEIVLYAYFKKLDYFSTECIYSPNAYR 240

Query: 253 GFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYISSQKWCKACVLL 312
           G AR F+KDLE IRP +I+DII SGE+  ++   +MP QGTC RCGYISSQ  CKACVLL
Sbjct: 241 GHARVFLKDLEAIRPSSIIDIIHSGENLSVNEDVRMPVQGTCTRCGYISSQSLCKACVLL 300

Query: 313 EGLNRGLPKLGIGRSRVLDNE 334
           EGLNRGLPKLGIG+   L ++
Sbjct: 301 EGLNRGLPKLGIGKHHKLHHK 321

[Close]

BLAST of Sed0011348.1 vs. ExPASy TrEMBL
Match: A0A6J1KNU4 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111496254 PE=3 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 2.0e-195
Identity = 342/361 (94.74%), Postives = 348/361 (96.40%), Query Frame = 0


Query: 1   MEEGGAKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFR 60
           MEE GAKP +KGV C+CCIC+NRKAALKRPKTL QICRECFYEVFEEEIHQVIVKN LFR
Sbjct: 1   MEEAGAKP-RKGVSCLCCICDNRKAALKRPKTLQQICRECFYEVFEEEIHQVIVKNHLFR 60

Query: 61  PGERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120
           PGERIAVGASGGKDSTVLAYVLS LNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI
Sbjct: 61  PGERIAVGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120

Query: 121 QYGLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHN 180
           QYGLPLQIVSYK+LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKL TGHN
Sbjct: 121 QYGLPLQIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHN 180

Query: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYF 240
           ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKY YEKEIVMYAYFKKLDYF
Sbjct: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYF 240

Query: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYI 300
           STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIS+STKMPEQGTCERCGYI
Sbjct: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISTSTKMPEQGTCERCGYI 300

Query: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRVLDNE-GKREMKG-SSNGHTRSIESKQCGTLD 360
           SSQKWCKACVLLEGLNRGLPKLGIGRSR LDNE  KREMKG SS+G TRSIESKQCGTLD
Sbjct: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRGLDNETEKREMKGRSSDGQTRSIESKQCGTLD 360

[Close]

BLAST of Sed0011348.1 vs. ExPASy TrEMBL
Match: A0A0A0LKF0 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucumis sativus OX=3659 GN=NCS6 PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 6.3e-194
Identity = 335/359 (93.31%), Postives = 344/359 (95.82%), Query Frame = 0


Query: 1   MEEGGAKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFR 60
           MEE   KP K+ V  +CC+CNNRKAALKRPKTL QICRECFYEVFEEEIHQVIVKNQLFR
Sbjct: 1   MEEAAPKP-KRAVSSLCCLCNNRKAALKRPKTLQQICRECFYEVFEEEIHQVIVKNQLFR 60

Query: 61  PGERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120
           PGERIAVGASGGKDSTVLAYVLS LN+RHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI
Sbjct: 61  PGERIAVGASGGKDSTVLAYVLSELNQRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120

Query: 121 QYGLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHN 180
           QYGLPLQIVSYK+LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKL TGHN
Sbjct: 121 QYGLPLQIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHN 180

Query: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYF 240
           ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKY YEKEIVMYAYFKKLDYF
Sbjct: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYF 240

Query: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYI 300
           STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIS+STKMPEQGTCERCGYI
Sbjct: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISTSTKMPEQGTCERCGYI 300

Query: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRVLDNEGKREMKGSSNGHTRSIESKQCGTLDF 360
           SSQKWCKACVLLEGLNRGLPKLGIGRSR LD+EGKRE  GS+N HTRSIESKQCG+LDF
Sbjct: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRGLDSEGKRETIGSNNVHTRSIESKQCGSLDF 358

[Close]

BLAST of Sed0011348.1 vs. ExPASy TrEMBL
Match: A0A6J1GII7 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111454465 PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 6.3e-194
Identity = 341/361 (94.46%), Postives = 347/361 (96.12%), Query Frame = 0


Query: 1   MEEGGAKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFR 60
           MEE GAK S++GV C+C ICNNRKAALKRPKTL QICRECFYEVFEEEIHQVIVKN LFR
Sbjct: 1   MEEAGAK-SRRGVSCLCGICNNRKAALKRPKTLQQICRECFYEVFEEEIHQVIVKNHLFR 60

Query: 61  PGERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120
           PGERIAVGASGGKDSTVLAYVLS LNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI
Sbjct: 61  PGERIAVGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120

Query: 121 QYGLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHN 180
           QYGLPLQIVSYK+LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKL TGHN
Sbjct: 121 QYGLPLQIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHN 180

Query: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYF 240
           ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKY YEKEIVMYAYFKKLDYF
Sbjct: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYF 240

Query: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYI 300
           STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIS+STKMPEQGTCERCGYI
Sbjct: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISTSTKMPEQGTCERCGYI 300

Query: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRVLDNE-GKREMKG-SSNGHTRSIESKQCGTLD 360
           SSQKWCKACVLLEGLNRGLPKLGIGRSR LDNE  KREMKG SS+G TRSIESKQCGTLD
Sbjct: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRGLDNETEKREMKGRSSDGQTRSIESKQCGTLD 360

[Close]

BLAST of Sed0011348.1 vs. ExPASy TrEMBL
Match: A0A6J1CSW4 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Momordica charantia OX=3673 GN=LOC111014001 PE=3 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.4e-193
Identity = 336/359 (93.59%), Postives = 344/359 (95.82%), Query Frame = 0


Query: 1   MEEGGAKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFR 60
           MEEGGA+ +KKGV  +CCICNNRK +LKRPKTL QICRECFYEVFEEEIHQVIVKN LF+
Sbjct: 1   MEEGGAR-AKKGVSSLCCICNNRKPSLKRPKTLQQICRECFYEVFEEEIHQVIVKNHLFK 60

Query: 61  PGERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120
           PGERIAVGASGGKDSTVLAYVLS LNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI
Sbjct: 61  PGERIAVGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120

Query: 121 QYGLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHN 180
           QYGLPLQIVSYK+LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKL TGHN
Sbjct: 121 QYGLPLQIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHN 180

Query: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYF 240
           ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKY YEKEIVMYAYFKKLDYF
Sbjct: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYF 240

Query: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYI 300
           STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIS+STK PEQGTCERCGYI
Sbjct: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISTSTKXPEQGTCERCGYI 300

Query: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRVLDNEGKREMKGSSNGHTRSIESKQCGTLDF 360
           SSQKWCKACVLLEGLNRGLPKLGIGRSR LDNE KREMKG S GH +SIESKQCGTLDF
Sbjct: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRGLDNEEKREMKG-SGGHEKSIESKQCGTLDF 357

[Close]

BLAST of Sed0011348.1 vs. ExPASy TrEMBL
Match: A0A1S3BD41 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucumis melo OX=3656 GN=LOC103488373 PE=3 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 2.4e-193
Identity = 334/359 (93.04%), Postives = 344/359 (95.82%), Query Frame = 0


Query: 1   MEEGGAKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFR 60
           MEE G KP K+ V  +CC+CNNRKAALKRPKTL QICRECFYEVFEEEIHQVIVKNQLFR
Sbjct: 1   MEEAGPKP-KRPVSSLCCLCNNRKAALKRPKTLQQICRECFYEVFEEEIHQVIVKNQLFR 60

Query: 61  PGERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120
           PGERIAVGASGGKDSTVLAYVLS LN+RHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI
Sbjct: 61  PGERIAVGASGGKDSTVLAYVLSELNQRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEI 120

Query: 121 QYGLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHN 180
           QYGLPLQIVSYK+LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAA LKVDKL TGHN
Sbjct: 121 QYGLPLQIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAAWLKVDKLATGHN 180

Query: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYF 240
           ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKY YEKEIVMYAYFKKLDYF
Sbjct: 181 ADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYF 240

Query: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYI 300
           STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIS+STKMPEQGTCERCGYI
Sbjct: 241 STECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISTSTKMPEQGTCERCGYI 300

Query: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRVLDNEGKREMKGSSNGHTRSIESKQCGTLDF 360
           SSQKWCKACVLLEGLNRGLPKLGIGRSR LD+EGK+E  GS+N HTRSIESKQCG+LDF
Sbjct: 301 SSQKWCKACVLLEGLNRGLPKLGIGRSRGLDSEGKKETIGSNNVHTRSIESKQCGSLDF 358

[Close]

BLAST of Sed0011348.1 vs. TAIR 10
Match: AT2G44270.1 (repressor of lrx1 )

HSP 1 Score: 626.7 bits (1615), Expect = 1.1e-179
Identity = 301/354 (85.03%), Postives = 326/354 (92.09%), Query Frame = 0


Query: 6   AKPSKKGVPCICCICNNRKAALKRPKTLHQICRECFYEVFEEEIHQVIVKNQLFRPGERI 65
           AK  K     +CC+CN R+  LKRPKTL QICRECFYEVFEEEIHQVIV+N+LF+ GER+
Sbjct: 3   AKNKKAVASRLCCLCNLRRPVLKRPKTLQQICRECFYEVFEEEIHQVIVQNRLFKSGERV 62

Query: 66  AVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLP 125
           A+GASGGKDSTVLAYVLS LNRRHNYGLDLFLLS+DEGITGYRDDSLETVKRNE+QYGLP
Sbjct: 63  AIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSIDEGITGYRDDSLETVKRNEVQYGLP 122

Query: 126 LQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHNADDMA 185
           L+IVSYK+LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKV+KLVTGHNADD+A
Sbjct: 123 LKIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDIA 182

Query: 186 ETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFKKLDYFSTECI 245
           ETVLLNILRGDIARLSRCT+I TGEDGPIPRCKPFKY YEKEIVMYAYFKKLDYFSTECI
Sbjct: 183 ETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTECI 242

Query: 246 YSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCERCGYISSQKW 305
           YSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRI+++TKMPEQGTCERCGYISSQKW
Sbjct: 243 YSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIATTTKMPEQGTCERCGYISSQKW 302

Query: 306 CKACVLLEGLNRGLPKLGIGRSRVLDNEGKREMKGSSNGHTRSIESKQCGTLDF 360
           CKACVLLEGLNRGLPK+GIGR R ++ +  +E K       +SIESKQCG+LDF
Sbjct: 303 CKACVLLEGLNRGLPKMGIGRPRGVNGDHNKETK-KPGSVAKSIESKQCGSLDF 355

[Close]

BLAST of Sed0011348.1 vs. TAIR 10
Match: AT2G44270.2 (repressor of lrx1 )

HSP 1 Score: 588.2 bits (1515), Expect = 4.5e-168
Identity = 290/364 (79.67%), Postives = 316/364 (86.81%), Query Frame = 0


Query: 6   AKPSKKGVPCICCICNNRKAALKRPKTLHQ----------ICRECFYEVFEEEIHQVIVK 65
           AK  K     +CC+CN R+  LKRPKTL Q            RECFYEVFEEEIHQVIV+
Sbjct: 3   AKNKKAVASRLCCLCNLRRPVLKRPKTLQQQKLGTHFYTVSVRECFYEVFEEEIHQVIVQ 62

Query: 66  NQLFRPGERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLLSVDEGITGYRDDSLETV 125
           N+LF+ GER+A+GASGGKDSTVLAYVLS LNRRHNYGLDLFLLS+DEGITGYRDDSLETV
Sbjct: 63  NRLFKSGERVAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSIDEGITGYRDDSLETV 122

Query: 126 KRNEIQYGLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKL 185
           KRNE+QYGLPL+IVSYK+LYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKV+KL
Sbjct: 123 KRNEVQYGLPLKIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKL 182

Query: 186 VTGHNADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFKYAYEKEIVMYAYFK 245
           VTGHNADD+AETVLLNILRGDIARLSRCT+I TGEDGPIPRCKPF +        YAYFK
Sbjct: 183 VTGHNADDIAETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFNFT-------YAYFK 242

Query: 246 KLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRISSSTKMPEQGTCE 305
           KLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRI+++TKMPEQGTCE
Sbjct: 243 KLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIATTTKMPEQGTCE 302

Query: 306 RCGYISSQKWCKACVLLEGLNRGLPKLGIGRSRVLDNEGKREMKGSSNGHTRSIESKQCG 360
           RCGYISSQKWCKACVLLEGLNRGLPK+GIGR R ++ +  +E K       +SIESKQCG
Sbjct: 303 RCGYISSQKWCKACVLLEGLNRGLPKMGIGRPRGVNGDHNKETK-KPGSVAKSIESKQCG 358

[Close]

BLAST of Sed0011348.1 vs. TAIR 10
Match: AT1G76170.1 (2-thiocytidine tRNA biosynthesis protein, TtcA )

HSP 1 Score: 577.0 bits (1486), Expect = 1.0e-164
Identity = 279/321 (86.92%), Postives = 300/321 (93.46%), Query Frame = 0


Query: 39  ECFYEVFEEEIHQVIVKNQLFRPGERIAVGASGGKDSTVLAYVLSNLNRRHNYGLDLFLL 98
           ECFYEVFEEEIHQVIV N+LF+ GER+A+GASGGKDS+VLAYVLS LNRRH+YGLDLFLL
Sbjct: 14  ECFYEVFEEEIHQVIVGNRLFKFGERVAIGASGGKDSSVLAYVLSELNRRHSYGLDLFLL 73

Query: 99  SVDEGITGYRDDSLETVKRNEIQYGLPLQIVSYKELYGWTMDEIVKMIGLKNNCTFCGVF 158
           S+DEGITGYRDDSLETVKRNE+QYGLPLQIVSYK+LYGWTMDEIVKMIGLKNNCTFCGVF
Sbjct: 74  SIDEGITGYRDDSLETVKRNEVQYGLPLQIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVF 133

Query: 159 RRQALDRGAALLKVDKLVTGHNADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCK 218
           RRQALDRGAALLKVDKLVTGHNADD+AETV+LNILRGDIARLSRCT+I TGEDGPIPRCK
Sbjct: 134 RRQALDRGAALLKVDKLVTGHNADDIAETVVLNILRGDIARLSRCTSITTGEDGPIPRCK 193

Query: 219 PFKYAYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGE 278
           PFKY YEKEIVMYAYFKKLDYFSTECIYSPNAYRGFA EFI DLER+RPRAILDIIKSGE
Sbjct: 194 PFKYTYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFACEFINDLERLRPRAILDIIKSGE 253

Query: 279 DFRISSSTKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPKLGIGRSRVLDNEGKREM 338
           DFRI+++TKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPK+GIGR R   N   +++
Sbjct: 254 DFRIATTTKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPKMGIGRPRGNVNGDYKDI 313

Query: 339 KGSSNGHTRSIESKQCGTLDF 360
           K  S   T  IESKQCG+LDF
Sbjct: 314 KARSTAKT--IESKQCGSLDF 332

The following BLAST results are available for this feature:

BLAST of Sed0011348.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023002400.1	4.1e-195	94.74	cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita maxima]	[more]
XP_038886415.1	1.2e-194	93.87	cytoplasmic tRNA 2-thiolation protein 1 [Benincasa hispida]	[more]
XP_023538431.1	1.5e-194	94.74	cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita pepo subsp. pepo] >XP_0235384...	[more]
KAG6585514.1	1.0e-193	94.46	Cytoplasmic tRNA 2-thiolation protein 1, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022951723.1	1.3e-193	94.46	cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita moschata]	[more]

BLAST of Sed0011348.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64862	1.6e-178	85.03	Cytoplasmic tRNA 2-thiolation protein 1 OS=Arabidopsis thaliana OX=3702 GN=NCS6 ...	[more]
Q6Z6G6	8.3e-167	79.89	Cytoplasmic tRNA 2-thiolation protein 1 OS=Oryza sativa subsp. japonica OX=39947...	[more]
A8JF71	3.3e-139	73.50	Cytoplasmic tRNA 2-thiolation protein 1 OS=Chlamydomonas reinhardtii OX=3055 GN=...	[more]
Q5FW05	4.3e-139	71.96	Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus tropicalis OX=8364 GN=ctu1 PE...	[more]
Q05AW7	4.0e-137	71.03	Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus laevis OX=8355 GN=ctu1 PE=2 S...	[more]

BLAST of Sed0011348.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KNU4	2.0e-195	94.74	Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111496...	[more]
A0A0A0LKF0	6.3e-194	93.31	Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucumis sativus OX=3659 GN=NCS6 PE=3 ...	[more]
A0A6J1GII7	6.3e-194	94.46	Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1CSW4	1.4e-193	93.59	Cytoplasmic tRNA 2-thiolation protein 1 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A1S3BD41	2.4e-193	93.04	Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucumis melo OX=3656 GN=LOC103488373 ...	[more]

BLAST of Sed0011348.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44270.1	1.1e-179	85.03	repressor of lrx1	[more]
AT2G44270.2	4.5e-168	79.67	repressor of lrx1	[more]
AT1G76170.1	1.0e-164	86.92	2-thiocytidine tRNA biosynthesis protein, TtcA	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 16..336 e-value: 2.8E-96 score: 324.3
IPR032442	Cytoplasmic tRNA 2-thiolation protein 1, C-terminal	PFAM	PF16503	zn-ribbon_14	coord: 292..322 e-value: 1.6E-18 score: 65.8
IPR011063	tRNA(Ile)-lysidine/2-thiocytidine synthase, N-terminal	PFAM	PF01171	ATP_bind_3	coord: 65..250 e-value: 4.0E-15 score: 56.0
IPR035107	tRNA thiolation protein, TtcA/Ctu1 type	PIRSF	PIRSF004976	ATPase_YdaO	coord: 14..324 e-value: 7.5E-126 score: 417.1
IPR000541	Cytoplasmic tRNA 2-thiolation protein 1	TIGRFAM	TIGR00269	TIGR00269	coord: 214..314 e-value: 4.0E-19 score: 66.8
IPR000541	Cytoplasmic tRNA 2-thiolation protein 1	PANTHER	PTHR11807:SF12	CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1	coord: 17..343
IPR000541	Cytoplasmic tRNA 2-thiolation protein 1	HAMAP	MF_03053	CTU1	coord: 15..327 score: 52.758972
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 344..359
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 333..359
None	No IPR available	PANTHER	PTHR11807	ATPASES OF THE PP SUPERFAMILY-RELATED	coord: 17..343
None	No IPR available	CDD	cd01993	Alpha_ANH_like_II	coord: 64..250 e-value: 4.9527E-94 score: 276.436
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 50..280

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sed0011348	Sed0011348	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0011348.1-exon	Sed0011348.1-exon-LG11:26939112..26939648	exon
Sed0011348.1-exon	Sed0011348.1-exon-LG11:26940378..26940489	exon
Sed0011348.1-exon	Sed0011348.1-exon-LG11:26940606..26940672	exon
Sed0011348.1-exon	Sed0011348.1-exon-LG11:26941752..26941792	exon
Sed0011348.1-exon	Sed0011348.1-exon-LG11:26941907..26941999	exon
Sed0011348.1-exon	Sed0011348.1-exon-LG11:26942095..26942207	exon
Sed0011348.1-exon	Sed0011348.1-exon-LG11:26943483..26943602	exon
Sed0011348.1-exon	Sed0011348.1-exon-LG11:26943880..26944022	exon
Sed0011348.1-exon	Sed0011348.1-exon-LG11:26944102..26944216	exon
Sed0011348.1-exon	Sed0011348.1-exon-LG11:26944672..26945017	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0011348.1-three_prime_utr	Sed0011348.1-three_prime_utr-LG11:26939112..26939477	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0011348.1-cds	Sed0011348.1-cds-LG11:26939478..26939648	CDS
Sed0011348.1-cds	Sed0011348.1-cds-LG11:26940378..26940489	CDS
Sed0011348.1-cds	Sed0011348.1-cds-LG11:26940606..26940672	CDS
Sed0011348.1-cds	Sed0011348.1-cds-LG11:26941752..26941792	CDS
Sed0011348.1-cds	Sed0011348.1-cds-LG11:26941907..26941999	CDS
Sed0011348.1-cds	Sed0011348.1-cds-LG11:26942095..26942207	CDS
Sed0011348.1-cds	Sed0011348.1-cds-LG11:26943483..26943602	CDS
Sed0011348.1-cds	Sed0011348.1-cds-LG11:26943880..26944022	CDS
Sed0011348.1-cds	Sed0011348.1-cds-LG11:26944102..26944216	CDS
Sed0011348.1-cds	Sed0011348.1-cds-LG11:26944672..26944776	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0011348.1-five_prime_utr	Sed0011348.1-five_prime_utr-LG11:26944777..26945017	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sed0011348.1	Sed0011348.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008033	tRNA processing
biological_process	GO:0034227	tRNA thio-modification
biological_process	GO:0002098	tRNA wobble uridine modification
biological_process	GO:0032447	protein urmylation
biological_process	GO:0002143	tRNA wobble position uridine thiolation
cellular_component	GO:0002144	cytosolic tRNA wobble base thiouridylase complex
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0016779	nucleotidyltransferase activity