Sed0009448.1 (mRNA) Chayote v1

Overview
NameSed0009448.1
TypemRNA
OrganismSechium edule (Chayote v1)
Descriptionpolygalacturonase 1 beta-like protein 3
LocationLG08: 757274 .. 759166 (+)
Sequence length1845
RNA-Seq ExpressionSed0009448.1
SyntenySed0009448.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCATTTCGTCTCCAATGCAAACAAACAAATCCCATTTTCTTCTCTGTTTCTATTCCTTCTTATTAATCCATTAATTTTGTCCTTTTTCCCACAAATTTCAATCGCTTCTTCGATTGACCAGTCCAGTCCCTTCACCGCCAAAGCTTCGCTAATTCGCTACTGGAACTCACACATTTCCAACCAATTTCCCAAGCCAGATTTCCTCTTCTCCAAATCCTCCCCTCTCTCCGCCGTCGACGCCGCCGTGTTCGCCGCCGCAGCCGCCGCCGGCAACCTCTCTTCCCGCCTCGAATCGTTCTGTTCTTCGGCGAATCTGTTCTGTTCGTTCGATTCAGAATCGCGTTCCAAGGCCGTTGCCGCGGATTTCAAGGCGTACGGAAACAGAAATTTCGTGAACTACGGCGCGGCTCGGCTAGGTGGCGCTGACTCGTTCAAGAATTACTCGACCGGGTTCAACTCGGCCGACCACTCGTTCAAGCGGTACAGCGGTAAATCGAATAATCACGGCGAGGGATTTACTAACTATGCGGAGGACGGAAATGTTGCGACGTCTGATTTTGTGAGTTACGGTACCGACTCGAAGGGCGGCTCGGGCGAGTTTTCGAGCTACGAGAAGCTGGTCAATGTGCCGAATCTTCGGTTCACTGCTTATGATTTGAATGCCAGAACTCATAAGCTCTCGTTTACGAGTTACGGCAACGAGACGAATTCGGGGAGTCAATCGTTTACGAGCTATGCGAAGAATGGCAATGGCGATCCTCAGGAGTTTAGTAGCTACTCGGATGGAGCCAACATTCTTACCTCCACGTTTACGGGCTATGGCGAGGCGGGGAAGAGCGCCAACGATTCGTTCAAGGCGTATGGACTATCTGCCAACAATCCGCACACGAACTTTAAGAGCTACGGGAACGGAGGAACTTCTCAGATTGATACATTCTCGAATTATAGAAACGGAGCCAATGTTGGAGAGGATAATTTCCAGTCGTATGCCAAGAACTCCAGCTCTGGAACTGTGAACTTTGCCAATTATGGCAAATCATTCAACCACGGAAACGATTCTTTCAAGGAATACGGCCGAGGATCGATTGGAAAAACGACCGTTGGATTCAAAAGTTACAGCATGGATCGGAATTTCACTACGTATGCACAAAATGGCGTTTCGTTTTCGGAGTATAGTAATTTTACTACCGCGACCGGTAGTGGCATTCACGTAAATAAGTACGTGGAGCCGGGTAAATTCTTTAGAGAGTTAGAATTGAAGGAAGGGAATGTCATGGTGATGCCGGATATTAGAGACAAAATGCCACCCAGATCGTTTCTTCCCAGGTCTATTTTGGTAAATTTACCCTTTTCCTCGTCGAGCCTCAACGAGACCAAGCAAATTTTCCACGCCCGAGATGGTTCGACGATGGAGCACGTGATAACCAACGCGCTAGCCGAGTGCGAGCGGGCGCCGAGCCAGGGCGAGACCAAGCGGTGCGTGGGCTCGGCGGAGGACATGATAGACTTTGCCACTGCAGTACTTGGACGGAATGTGGTTGCAAGGACGACAGCTAATGTGAATGGGTCAGCAGAGAAGGTAGAGATCGGGACAGTCAAAGGAATCAACGGTGGAGATTTAACAAGATCGGTATCGTGCCATCAAAGTTTGTATCCGTACTTACTGTATTACTGCCACTCAGTCCCAAAAGTCCGTATATACGAAGCAGATATCCTGAGGCTGAAAACAAAGGAAACGATCAATCGTGGGGTTGCCATCTGTCACATAGACACGTCATCGTGGGGTCCAGAGCATGGTGCTTTCGTGGCACTCGGGTCCGGTCCAGGTCAAATAGAAGTCTGCCACTGGATATTCCAGAACGACATGTCGTGGAGTGTTGCTGACTGA

mRNA sequence

ATGGCCCATTTCGTCTCCAATGCAAACAAACAAATCCCATTTTCTTCTCTGTTTCTATTCCTTCTTATTAATCCATTAATTTTTCCCTTCACCGCCAAAGCTTCGCTAATTCGCTACTGGAACTCACACATTTCCAACCAATTTCCCAAGCCAGATTTCCTCTTCTCCAAATCCTCCCCTCTCTCCGCCGTCGACGCCGCCGTGTTCGCCGCCGCAGCCGCCGCCGGCAACCTCTCTTCCCGCCTCGAATCGTTCTGTTCTTCGGCGAATCTGTTCTGTTCGTTCGATTCAGAATCGCGTTCCAAGGCCGTTGCCGCGGATTTCAAGGCGTACGGAAACAGAAATTTCGTGAACTACGGCGCGGCTCGGCTAGGTGGCGCTGACTCGTTCAAGAATTACTCGACCGGGTTCAACTCGGCCGACCACTCGTTCAAGCGGTACAGCGGTAAATCGAATAATCACGGCGAGGGATTTACTAACTATGCGGAGGACGGAAATGTTGCGACGTCTGATTTTGTGAGTTACGGTACCGACTCGAAGGGCGGCTCGGGCGAGTTTTCGAGCTACGAGAAGCTGGTCAATGTGCCGAATCTTCGGTTCACTGCTTATGATTTGAATGCCAGAACTCATAAGCTCTCGTTTACGAGTTACGGCAACGAGACGAATTCGGGGAGTCAATCGTTTACGAGCTATGCGAAGAATGGCAATGGCGATCCTCAGGAGTTTAGTAGCTACTCGGATGGAGCCAACATTCTTACCTCCACGTTTACGGGCTATGGCGAGGCGGGGAAGAGCGCCAACGATTCGTTCAAGGCGTATGGACTATCTGCCAACAATCCGCACACGAACTTTAAGAGCTACGGGAACGGAGGAACTTCTCAGATTGATACATTCTCGAATTATAGAAACGGAGCCAATGTTGGAGAGGATAATTTCCAGTCGTATGCCAAGAACTCCAGCTCTGGAACTGTGAACTTTGCCAATTATGGCAAATCATTCAACCACGGAAACGATTCTTTCAAGGAATACGGCCGAGGATCGATTGGAAAAACGACCGTTGGATTCAAAAGTTACAGCATGGATCGGAATTTCACTACGTATGCACAAAATGGCGTTTCGTTTTCGGAGTATAGTAATTTTACTACCGCGACCGGTAGTGGCATTCACGTAAATAAGTACGTGGAGCCGGGTAAATTCTTTAGAGAGTTAGAATTGAAGGAAGGGAATGTCATGGTGATGCCGGATATTAGAGACAAAATGCCACCCAGATCGTTTCTTCCCAGGTCTATTTTGGTAAATTTACCCTTTTCCTCGTCGAGCCTCAACGAGACCAAGCAAATTTTCCACGCCCGAGATGGTTCGACGATGGAGCACGTGATAACCAACGCGCTAGCCGAGTGCGAGCGGGCGCCGAGCCAGGGCGAGACCAAGCGGTGCGTGGGCTCGGCGGAGGACATGATAGACTTTGCCACTGCAGTACTTGGACGGAATGTGGTTGCAAGGACGACAGCTAATGTGAATGGGTCAGCAGAGAAGGTAGAGATCGGGACAGTCAAAGGAATCAACGGTGGAGATTTAACAAGATCGGTATCGTGCCATCAAAGTTTGTATCCGTACTTACTGTATTACTGCCACTCAGTCCCAAAAGTCCGTATATACGAAGCAGATATCCTGAGGCTGAAAACAAAGGAAACGATCAATCGTGGGGTTGCCATCTGTCACATAGACACGTCATCGTGGGGTCCAGAGCATGGTGCTTTCGTGGCACTCGGGTCCGGTCCAGGTCAAATAGAAGTCTGCCACTGGATATTCCAGAACGACATGTCGTGGAGTGTTGCTGACTGA

Coding sequence (CDS)

ATGGCCCATTTCGTCTCCAATGCAAACAAACAAATCCCATTTTCTTCTCTGTTTCTATTCCTTCTTATTAATCCATTAATTTTTCCCTTCACCGCCAAAGCTTCGCTAATTCGCTACTGGAACTCACACATTTCCAACCAATTTCCCAAGCCAGATTTCCTCTTCTCCAAATCCTCCCCTCTCTCCGCCGTCGACGCCGCCGTGTTCGCCGCCGCAGCCGCCGCCGGCAACCTCTCTTCCCGCCTCGAATCGTTCTGTTCTTCGGCGAATCTGTTCTGTTCGTTCGATTCAGAATCGCGTTCCAAGGCCGTTGCCGCGGATTTCAAGGCGTACGGAAACAGAAATTTCGTGAACTACGGCGCGGCTCGGCTAGGTGGCGCTGACTCGTTCAAGAATTACTCGACCGGGTTCAACTCGGCCGACCACTCGTTCAAGCGGTACAGCGGTAAATCGAATAATCACGGCGAGGGATTTACTAACTATGCGGAGGACGGAAATGTTGCGACGTCTGATTTTGTGAGTTACGGTACCGACTCGAAGGGCGGCTCGGGCGAGTTTTCGAGCTACGAGAAGCTGGTCAATGTGCCGAATCTTCGGTTCACTGCTTATGATTTGAATGCCAGAACTCATAAGCTCTCGTTTACGAGTTACGGCAACGAGACGAATTCGGGGAGTCAATCGTTTACGAGCTATGCGAAGAATGGCAATGGCGATCCTCAGGAGTTTAGTAGCTACTCGGATGGAGCCAACATTCTTACCTCCACGTTTACGGGCTATGGCGAGGCGGGGAAGAGCGCCAACGATTCGTTCAAGGCGTATGGACTATCTGCCAACAATCCGCACACGAACTTTAAGAGCTACGGGAACGGAGGAACTTCTCAGATTGATACATTCTCGAATTATAGAAACGGAGCCAATGTTGGAGAGGATAATTTCCAGTCGTATGCCAAGAACTCCAGCTCTGGAACTGTGAACTTTGCCAATTATGGCAAATCATTCAACCACGGAAACGATTCTTTCAAGGAATACGGCCGAGGATCGATTGGAAAAACGACCGTTGGATTCAAAAGTTACAGCATGGATCGGAATTTCACTACGTATGCACAAAATGGCGTTTCGTTTTCGGAGTATAGTAATTTTACTACCGCGACCGGTAGTGGCATTCACGTAAATAAGTACGTGGAGCCGGGTAAATTCTTTAGAGAGTTAGAATTGAAGGAAGGGAATGTCATGGTGATGCCGGATATTAGAGACAAAATGCCACCCAGATCGTTTCTTCCCAGGTCTATTTTGGTAAATTTACCCTTTTCCTCGTCGAGCCTCAACGAGACCAAGCAAATTTTCCACGCCCGAGATGGTTCGACGATGGAGCACGTGATAACCAACGCGCTAGCCGAGTGCGAGCGGGCGCCGAGCCAGGGCGAGACCAAGCGGTGCGTGGGCTCGGCGGAGGACATGATAGACTTTGCCACTGCAGTACTTGGACGGAATGTGGTTGCAAGGACGACAGCTAATGTGAATGGGTCAGCAGAGAAGGTAGAGATCGGGACAGTCAAAGGAATCAACGGTGGAGATTTAACAAGATCGGTATCGTGCCATCAAAGTTTGTATCCGTACTTACTGTATTACTGCCACTCAGTCCCAAAAGTCCGTATATACGAAGCAGATATCCTGAGGCTGAAAACAAAGGAAACGATCAATCGTGGGGTTGCCATCTGTCACATAGACACGTCATCGTGGGGTCCAGAGCATGGTGCTTTCGTGGCACTCGGGTCCGGTCCAGGTCAAATAGAAGTCTGCCACTGGATATTCCAGAACGACATGTCGTGGAGTGTTGCTGACTGA

Protein sequence

MAHFVSNANKQIPFSSLFLFLLINPLIFPFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSSANLFCSFDSESRSKAVAADFKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFKRYSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDLNARTHKLSFTSYGNETNSGSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKSANDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVNFANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTTATGSGIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLNETKQIFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKETINRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD
Homology
BLAST of Sed0009448.1 vs. NCBI nr
Match: XP_038878610.1 (polygalacturonase 1 beta-like protein 3 [Benincasa hispida])

HSP 1 Score: 1007.7 bits (2604), Expect = 4.4e-290
Identity = 504/589 (85.57%), Postives = 537/589 (91.17%), Query Frame = 0

Query: 29  PFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSS 88
           PFTAKASL+RYWNSH+SNQ P PDFLFSK+SPLSAVDAAV A  AA G+LS RLESFC S
Sbjct: 32  PFTAKASLLRYWNSHVSNQLPPPDFLFSKASPLSAVDAAVLAQHAARGSLSFRLESFCYS 91

Query: 89  ANLFCSFDSE---SRSKAVAADFKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFK 148
           ANLFCSFDSE   S  +   ADFK YGN+NFVNYG ARLGG DSFKNYS G N+ADHSFK
Sbjct: 92  ANLFCSFDSETEISVPRLKDADFKLYGNKNFVNYGGARLGGTDSFKNYSDGLNTADHSFK 151

Query: 149 RYSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDL 208
           RY G S+NH EGFTNYAEDGNVA S+F+SY T+SKGGSGEF +Y+KLVNVPNLRFTAYDL
Sbjct: 152 RYGGNSSNHEEGFTNYAEDGNVANSNFISYATESKGGSGEFVNYDKLVNVPNLRFTAYDL 211

Query: 209 NARTHKLSFTSYGNETNSGSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKS 268
           N+R HKLSFT YGNETNSGSQ+FTSY K GNG PQEFSSYSDG+NILTSTFTGYGE+GK+
Sbjct: 212 NSRNHKLSFTGYGNETNSGSQTFTSYGKKGNGVPQEFSSYSDGSNILTSTFTGYGESGKT 271

Query: 269 ANDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVN 328
           ANDSFK YG+S NNPHTNFK+YG GG S+IDTFSNYRNGANVG+D+FQSYAKNS+SG VN
Sbjct: 272 ANDSFKGYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVN 331

Query: 329 FANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTTATG 388
           FANYGKSFNHGNDSF EYG+GSIG+TTVGFKSYS+DRNFTTYAQ GVSFSEYSNFTTATG
Sbjct: 332 FANYGKSFNHGNDSFTEYGKGSIGRTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATG 391

Query: 389 SGIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLNETK 448
           SGIHVNK VEPGKFFRE ELK+GNVMVMPDIRDKMPPRSFLPR ILVNLPFSSS ++E K
Sbjct: 392 SGIHVNKLVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAK 451

Query: 449 QIFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVARTTAN 508
           +IFHARDGSTME VITNAL ECERAPS GETKRCVGSAEDMIDFAT VLGR VVARTTAN
Sbjct: 452 EIFHARDGSTMERVITNALGECERAPSPGETKRCVGSAEDMIDFATTVLGRRVVARTTAN 511

Query: 509 VNGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKET 568
           VNGS EKVEIGTVKGINGGDLT SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKE 
Sbjct: 512 VNGSREKVEIGTVKGINGGDLTSSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEK 571

Query: 569 INRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           INRGVAICH+DTSSWGPEHGAFVALGSGPGQIEVCHWIF+NDMSWSVAD
Sbjct: 572 INRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 620

BLAST of Sed0009448.1 vs. NCBI nr
Match: XP_022921994.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita moschata])

HSP 1 Score: 995.0 bits (2571), Expect = 2.9e-286
Identity = 495/588 (84.18%), Postives = 545/588 (92.69%), Query Frame = 0

Query: 29  PFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSS 88
           PFTAKASL+RYWN+H+SNQ PKPDFLF+K+SPLSAVDAAVFA  AA G+L+SRLE+FC S
Sbjct: 43  PFTAKASLVRYWNTHVSNQLPKPDFLFAKASPLSAVDAAVFAKHAALGSLTSRLETFCYS 102

Query: 89  ANLFCSFDSESRS--KAVAADFKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFKR 148
           ANLFCSFDS+  S  +   ADFK YGN+NF NYGAAR+GGADSFKNYS G N+ADHSFKR
Sbjct: 103 ANLFCSFDSQETSVPRFKDADFKLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKR 162

Query: 149 YSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDLN 208
           Y G S+NH EGFTNYAEDGNVA ++F+SY  +SKGGSGEF +Y+K+VNVPNLRFTAYDL+
Sbjct: 163 YGGNSSNHKEGFTNYAEDGNVANNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLD 222

Query: 209 ARTHKLSFTSYGNETNSGSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKSA 268
           +R HKLSFTSYGNETNSGSQSFTSY KNGNG PQEFSSYSDG+NILTSTFTGYGE+GKSA
Sbjct: 223 SRAHKLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSA 282

Query: 269 NDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVNF 328
           NDSFKAYGLS NNPH+NFK+YG GG SQIDTFSNYRNGANVG+D+FQSYAKNS+SG VNF
Sbjct: 283 NDSFKAYGLSGNNPHSNFKNYGKGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNF 342

Query: 329 ANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTTATGS 388
           A+YG+SFNHGNDSFKEYG+GSIG+TTVGF+SYS+DRNFTTYAQ GVSFSEYS    ATGS
Sbjct: 343 ASYGQSFNHGNDSFKEYGKGSIGQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGS 402

Query: 389 GIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLNETKQ 448
           GI+VN++VEPGKFFRE ELK+GNVMVMPDIRDKMPPRSFLPR+ILVN+PFSSS ++ETKQ
Sbjct: 403 GINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQ 462

Query: 449 IFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVARTTANV 508
           IFHAR+GSTMEHVIT+ALAECERAPS GETKRCVGSAEDMIDFATAVLGRNVV RTTA+V
Sbjct: 463 IFHARNGSTMEHVITSALAECERAPSMGETKRCVGSAEDMIDFATAVLGRNVVVRTTASV 522

Query: 509 NGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKETI 568
           NGS EKVEIG VKGINGG+LTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT E I
Sbjct: 523 NGSKEKVEIGEVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKI 582

Query: 569 NRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           NRGVAICH+DTSSWGPEHGAFVALGSGPGQIEVCHWIF+NDMSWSVAD
Sbjct: 583 NRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of Sed0009448.1 vs. NCBI nr
Match: KAG6589452.1 (Polygalacturonase 1 beta-like protein 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023135.1 Polygalacturonase 1 beta-like protein 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 994.6 bits (2570), Expect = 3.8e-286
Identity = 495/588 (84.18%), Postives = 545/588 (92.69%), Query Frame = 0

Query: 29  PFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSS 88
           PFTAKASL+RYWN+H+SNQ PKPDFLF+K+SPLSAVDAAVFA  AA G+L+SRLE+FC S
Sbjct: 43  PFTAKASLVRYWNTHVSNQLPKPDFLFAKASPLSAVDAAVFAKHAALGSLTSRLETFCYS 102

Query: 89  ANLFCSFDSESRS--KAVAADFKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFKR 148
           ANLFCSFDS+  S  +   ADFK YGN+NF NYGAAR+GGADSFKNYS G N+ADHSFKR
Sbjct: 103 ANLFCSFDSQETSIPRFKDADFKLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKR 162

Query: 149 YSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDLN 208
           Y G S+NH EGF NYAEDGNVA ++F+SY  +SKGGSGEF +Y+K+VNVPNLRFTAYDL+
Sbjct: 163 YGGNSSNHKEGFMNYAEDGNVANNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLD 222

Query: 209 ARTHKLSFTSYGNETNSGSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKSA 268
           +R HKLSFTSYGNETNSGSQSFTSY KNGNG PQEFSSYSDG+NILTSTFTGYGE+GKSA
Sbjct: 223 SRAHKLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSA 282

Query: 269 NDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVNF 328
           NDSFKAYGLS NNPH+NFK+YG GG SQIDTFSNYRNGANVG+D+FQSYAKNS+SG VNF
Sbjct: 283 NDSFKAYGLSGNNPHSNFKNYGKGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNF 342

Query: 329 ANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTTATGS 388
           A+YG+SFNHGNDSFKEYG+GSIG+TTVGF+SYS+DRNFTTYAQ GVSFSEYS    ATGS
Sbjct: 343 ASYGQSFNHGNDSFKEYGKGSIGQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGS 402

Query: 389 GIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLNETKQ 448
           GI+VN++VEPGKFFRE ELK+GNVMVMPDIRDKMPPRSFLPR+ILVN+PFSSS ++ETKQ
Sbjct: 403 GINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQ 462

Query: 449 IFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVARTTANV 508
           IFHAR+GSTMEHVIT+ALAECERAPS GETKRCVGSAEDMIDFATAVLGRNVVARTTA+V
Sbjct: 463 IFHARNGSTMEHVITSALAECERAPSMGETKRCVGSAEDMIDFATAVLGRNVVARTTASV 522

Query: 509 NGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKETI 568
           NGS EKVEIG VKGINGG+LTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT E I
Sbjct: 523 NGSTEKVEIGEVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKI 582

Query: 569 NRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           NRGVAICH+DTSSWGPEHGAFVALGSGPGQIEVCHWIF+NDMSWSVAD
Sbjct: 583 NRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of Sed0009448.1 vs. NCBI nr
Match: XP_004138283.1 (polygalacturonase 1 beta-like protein 3 [Cucumis sativus] >KGN63588.1 hypothetical protein Csa_013467 [Cucumis sativus])

HSP 1 Score: 993.8 bits (2568), Expect = 6.5e-286
Identity = 499/631 (79.08%), Postives = 552/631 (87.48%), Query Frame = 0

Query: 1   MAHFVSNANKQIPFSSLFLFLLINPLIF--------------PFTAKASLIRYWNSHISN 60
           ++ F    NKQI F  LFLFLL  P IF              PFT KASL+RYW +H+S 
Sbjct: 17  LSFFHLTTNKQISFKFLFLFLL--PFIFFLSFFPQISSASSNPFTPKASLVRYWKTHVST 76

Query: 61  QFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSSANLFCSFDSESRS---KA 120
             P PDF  SK+SPLSAVDAA  A  AA  +LSSRLESFC SANLFCSFDS+S +   ++
Sbjct: 77  DLPTPDFFLSKASPLSAVDAAALATHAAGASLSSRLESFCFSANLFCSFDSQSETSVPRS 136

Query: 121 VAADFKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFKRYSGKSNNHGEGFTNYAE 180
             ADFK YGN+NFVNYG +RLGG DSFKNYS G N+A+HSFKRY G S+NH EGFT+YAE
Sbjct: 137 KDADFKLYGNKNFVNYGGSRLGGTDSFKNYSDGLNTAEHSFKRYGGNSSNHKEGFTSYAE 196

Query: 181 DGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDLNARTHKLSFTSYGNETNS 240
           DGNVA S+FVSYG  SK GSGEF++Y+KLVNVPNLRFTAYDLN+R+HKLSFTSYGNETNS
Sbjct: 197 DGNVANSNFVSYGAGSKSGSGEFTNYDKLVNVPNLRFTAYDLNSRSHKLSFTSYGNETNS 256

Query: 241 GSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKSANDSFKAYGLSANNPHTN 300
           GSQSFTSY KNGNG PQ+FSSYSDG+NILTSTFTGYGE+GK+ANDSFK YG+S NNPHTN
Sbjct: 257 GSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGESGKTANDSFKEYGISGNNPHTN 316

Query: 301 FKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVNFANYGKSFNHGNDSFKEY 360
           FK+YG GG S+IDTFSNYRNGANVG+D+FQSYAKNS+SG VNFANYGKSFNHGNDSF EY
Sbjct: 317 FKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEY 376

Query: 361 GRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTTATGSGIHVNKYVEPGKFFREL 420
           G+GS+GKTTVGFKSYS+DRNFTTYA+NGVSFS Y+NFTTATGSGIH N +VEPGKFFRE 
Sbjct: 377 GKGSMGKTTVGFKSYSVDRNFTTYAKNGVSFSVYNNFTTATGSGIHANSHVEPGKFFRES 436

Query: 421 ELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLNETKQIFHARDGSTMEHVITNA 480
           ELK+GNVMVMPDI+DKMPPRSFLPRSILVNLPFSSS ++E K++FHARDGSTME VITNA
Sbjct: 437 ELKQGNVMVMPDIKDKMPPRSFLPRSILVNLPFSSSRISEAKEMFHARDGSTMESVITNA 496

Query: 481 LAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSAEKVEIGTVKGING 540
           LAECERAPS+GETKRCVGSAEDMIDFAT VLG+ VV RTTANVNGS EK+EIG VKGING
Sbjct: 497 LAECERAPSRGETKRCVGSAEDMIDFATTVLGQRVVVRTTANVNGSKEKIEIGRVKGING 556

Query: 541 GDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKETINRGVAICHIDTSSWGPE 600
           GDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADIL+++TKE INRGVAICHIDTSSWGPE
Sbjct: 557 GDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKMETKEKINRGVAICHIDTSSWGPE 616

Query: 601 HGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           HGAFVALGSGPGQIEVCHWIF+NDMSW+VAD
Sbjct: 617 HGAFVALGSGPGQIEVCHWIFENDMSWNVAD 645

BLAST of Sed0009448.1 vs. NCBI nr
Match: XP_023516985.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 992.3 bits (2564), Expect = 1.9e-285
Identity = 493/588 (83.84%), Postives = 545/588 (92.69%), Query Frame = 0

Query: 29  PFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSS 88
           PFTAKASL+RYWN+H+SNQ PKPDFLF+K+SPLS VDAAVFA  AA G+L+SRLE+FC S
Sbjct: 43  PFTAKASLVRYWNTHVSNQLPKPDFLFAKASPLSVVDAAVFAKHAALGSLTSRLETFCYS 102

Query: 89  ANLFCSFDSESRS--KAVAADFKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFKR 148
           ANLFCSFDS+  S  +   ADFK YGN+NF NYGAAR+GGADSFKNYS G N+ADHSFKR
Sbjct: 103 ANLFCSFDSQETSVPRFKDADFKLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKR 162

Query: 149 YSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDLN 208
           YSG S+NH EGFTNYAEDGNVA ++F+SY  +SKGGSGEF +Y+K+VNVPNLRFTAYDL+
Sbjct: 163 YSGNSSNHKEGFTNYAEDGNVANNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLD 222

Query: 209 ARTHKLSFTSYGNETNSGSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKSA 268
           +R HKLSFTSYGNETNSGSQSFTSY KNGNG PQEFSSYSDG+NILTSTF+GYGE+GKSA
Sbjct: 223 SRAHKLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFSSYSDGSNILTSTFSGYGESGKSA 282

Query: 269 NDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVNF 328
           NDSFKAYGLS NNPH+NFK+YG GG SQIDTFSNYRNGANVG+D+FQSYAKNS+SG VNF
Sbjct: 283 NDSFKAYGLSGNNPHSNFKNYGKGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNF 342

Query: 329 ANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTTATGS 388
           A+YG+SFNHGNDSFKEYG+GSIG+TTVGF+SYS+DRNFTTYAQ GVSFSEYS    ATGS
Sbjct: 343 ASYGQSFNHGNDSFKEYGKGSIGQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGS 402

Query: 389 GIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLNETKQ 448
           GI+VN++VEPGKFFRE ELK+GNVMVMPDIRDKMPPRSFLPR+ILVN+PFSSS ++ETKQ
Sbjct: 403 GINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQ 462

Query: 449 IFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVARTTANV 508
           IFHAR+GSTMEHVIT+ LAECERAPS GETKRCVGSAEDMIDFATAVLGRNVVARTTA+V
Sbjct: 463 IFHARNGSTMEHVITSTLAECERAPSMGETKRCVGSAEDMIDFATAVLGRNVVARTTASV 522

Query: 509 NGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKETI 568
           NGS EKVEIG VKGINGG+LTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT E I
Sbjct: 523 NGSTEKVEIGEVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKI 582

Query: 569 NRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           NRGVAICH+DTSSWGP+HGAFVALGSGPGQIEVCHWIF+NDMSWSVAD
Sbjct: 583 NRGVAICHMDTSSWGPKHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of Sed0009448.1 vs. ExPASy Swiss-Prot
Match: P92990 (Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 PE=2 SV=2)

HSP 1 Score: 671.8 bits (1732), Expect = 7.5e-192
Identity = 346/592 (58.45%), Postives = 434/592 (73.31%), Query Frame = 0

Query: 29  PFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSS 88
           PFT KASL+RYWN  I  Q P+ +FL SK+SPL+AVD+A F+  AAA +L +R   FCS+
Sbjct: 36  PFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAANSLPTRFPDFCSA 95

Query: 89  ANLFCSFDSESRSKAVAAD--FKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFKR 148
           ANLFC  D  +  +    D  F  Y  +NF NYG AR GGADSFKNYS   N    SF+R
Sbjct: 96  ANLFCFPDLGASLEKHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGNVVTDSFRR 155

Query: 149 YSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDLN 208
           YS  +  H + FT Y E+ NV    F SYGT   GG+G+F++Y+  VN P  RFTAY   
Sbjct: 156 YSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTSRFTAYSDG 215

Query: 209 ARTHKLSFTSYGNETNSGS-QSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKS 268
                 +F +Y +E N+G+ QSFTSY KNGNG P EF+SY   +N++ S F+ YGE+G +
Sbjct: 216 GNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFSNYGESGNA 275

Query: 269 ANDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVN 328
           AND+F +YG   N P  NF +YG  G + +DTF+NYR+ ANVG+D+F SYAK+S+S  VN
Sbjct: 276 ANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAKDSNSEKVN 335

Query: 329 FANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTT--- 388
           F NYG+SFN G+++F  YG+G+ G + + FK+Y+ +  F  YA+ GV+F++Y+  TT   
Sbjct: 336 FVNYGQSFNPGSETFTGYGKGAEG-SKLSFKTYTPNSTFKDYAKKGVAFAKYNVSTTTAN 395

Query: 389 ATGSGIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLN 448
             G G  VNK++EPGKFFRE  LKEG V+ MPDI+DKMP RSFLPRSI+  LPFS+S L 
Sbjct: 396 TVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLPFSTSKLG 455

Query: 449 ETKQIFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVART 508
           E K+IFHA + STM  +IT+A+ ECER PS GETKRCVGSAEDMIDFAT+VLGR+VV RT
Sbjct: 456 EIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLGRSVVLRT 515

Query: 509 TANVNGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT 568
           T NV GS EKV IG V GINGG LT++VSCHQSLYPYLLYYCHSVPKVR+YEAD+L L +
Sbjct: 516 TENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLELNS 575

Query: 569 KETINRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           K+ IN G+AICH+DTSSWGP HGAF+ALGS PG+IEVCHWIF+NDM+W++AD
Sbjct: 576 KKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Sed0009448.1 vs. ExPASy Swiss-Prot
Match: O80760 (Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 PE=2 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 3.8e-188
Identity = 342/595 (57.48%), Postives = 427/595 (71.76%), Query Frame = 0

Query: 23  INPLIFPFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRL 82
           I P   PFT KASLIRYWN+HI+   PKP F  SK+SPL+AVD+  FA+ A+   L++  
Sbjct: 33  ITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPLTAVDSTRFASLASNHALNTHH 92

Query: 83  ESFCSSANLFCSFDSESRSKAVAAD---FKAYGNRNFVNYGAARLGGADSFKNYSTGFNS 142
             FCS+A LFC  +  + S     D   F AY  +NF NYG+ RL GADSFKNYS G N 
Sbjct: 93  SDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTNYGSDRLSGADSFKNYSGGDNI 152

Query: 143 ADHSFKRYSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLR 202
           A  SF+RYS  S  H +GFTNYA + NVA   F +Y T + GGSGEF++Y    N PN R
Sbjct: 153 AVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATGTTGGSGEFTNYNTDANEPNGR 212

Query: 203 FTAYDLNARTHKLSFTSYGNETNSGSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGY 262
           FT+Y   A     +FT+Y    N+G QSFTSY+KNGNG P EFS Y  G+N++ + FT Y
Sbjct: 213 FTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGAPNEFSGYGTGSNVVNTGFTKY 272

Query: 263 GEAGKSANDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNS 322
           GE+   ANDSF +YG + N P   FK YG+GG   +  F NYR+ +N+G D+F SYAKNS
Sbjct: 273 GESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGFKNYRDQSNIGVDSFSSYAKNS 332

Query: 323 SSGTVNFANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSN 382
           ++  VNF NYGKSFN G+D+F  YG+ ++G   V FK+Y   ++F  Y ++GV F+ YSN
Sbjct: 333 NNEKVNFVNYGKSFNLGSDNFTGYGQDNVG-GNVSFKTYGQGQSFKVYTKDGVVFARYSN 392

Query: 383 FTTATGSGIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSS 442
               + +G  VNK+VE GKFFRE  LKEG +M MPDI+DKMP R+FLPR+I+ NLPFSSS
Sbjct: 393 --NVSSNGKTVNKWVEEGKFFREAMLKEGTLMQMPDIKDKMPKRTFLPRNIVKNLPFSSS 452

Query: 443 SLNETKQIFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVV 502
           ++ E  ++F A + S+M  +I++A++ECER  S GETKRCVGSAEDMIDFAT+VLGR VV
Sbjct: 453 TIGEIWRVFGAGENSSMAGIISSAVSECERPASHGETKRCVGSAEDMIDFATSVLGRGVV 512

Query: 503 ARTTANVNGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILR 562
            RTT NV GS +KV IG V GINGGD+TR+VSCHQSLYPYLLYYCHSVP+VR+YE D+L 
Sbjct: 513 VRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQSLYPYLLYYCHSVPRVRVYETDLLD 572

Query: 563 LKTKETINRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
            K+ E IN GVAICHIDTS+W P HGAF+ALGSGPGQIEVCHWIF+NDM+W++ D
Sbjct: 573 PKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPGQIEVCHWIFENDMTWNIID 624

BLAST of Sed0009448.1 vs. ExPASy Swiss-Prot
Match: P92982 (Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 PE=2 SV=2)

HSP 1 Score: 651.0 bits (1678), Expect = 1.4e-185
Identity = 341/594 (57.41%), Postives = 421/594 (70.88%), Query Frame = 0

Query: 24  NPLIFPFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLE 83
           +P I PFT KASL+RYWN  I +Q P   FL SK+SPL+AV +A FA  A+   L  RL 
Sbjct: 32  SPEINPFTPKASLVRYWNKQIHHQSPGSAFLLSKASPLTAVASATFAKLASENALPDRLP 91

Query: 84  SFCSSANLFCSFDSESRSKAVAAD--FKAYGNRNFVNYGAARLGGADSFKNYSTGFNSAD 143
            FCS+ANLFC  D     +  + D  F  Y  RNF NYG    GGADSFK YS   N+  
Sbjct: 92  DFCSAANLFCFPDLGPTLEKHSNDVKFSVYDQRNFTNYGTIHPGGADSFKKYSQNGNAVT 151

Query: 144 HSFKRYSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFT 203
            SF+RYS  +  H + FT YA++ NV    F SYGT + GGSG+F +Y+  VN P  RF 
Sbjct: 152 DSFRRYSRDAAGHEDKFTGYADNSNVVEEKFNSYGTSATGGSGDFKNYQSGVNNPTTRFK 211

Query: 204 AYDLNARTHKLSFTSYGNETNSG-SQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYG 263
           AY         +F +Y +E NSG  QSFTSY KNGNG P EFSSY   +N++ S F+ YG
Sbjct: 212 AYSDEGNGRAQTFKTYTHEANSGPGQSFTSYGKNGNGAPNEFSSYGVSSNVIGSDFSNYG 271

Query: 264 EAGKSANDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSS 323
           E G +AND+FK YG   N P  NFKSYG  G + +DTF NYR+ ANVG+D+F SYAKNS+
Sbjct: 272 ENGNAANDTFKGYGGDGNVPQNNFKSYGASGNAAVDTFLNYRDNANVGDDSFSSYAKNSN 331

Query: 324 SGTVNFANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNF 383
              V+F NYGKS N G++SF  YG+G+ G   + FK+Y+ +  F  Y + GV F++Y+  
Sbjct: 332 FEKVDFVNYGKSINPGSESFSGYGKGAEG-NKIDFKTYTQNSTFKDYTKTGVEFAKYNR- 391

Query: 384 TTATGSGIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSS 443
            ++ G G  VNK+VEPGKFFRE  LKEG ++ MPDI+DKMP RSFLPRSI+  LPFS+S 
Sbjct: 392 -SSLGGGKTVNKWVEPGKFFRESMLKEGTLIWMPDIKDKMPKRSFLPRSIVSKLPFSTSK 451

Query: 444 LNETKQIFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVA 503
           + E K++FHA D STME +IT+A+ ECER P+  ETKRCVGSAEDMIDFAT+VLGR+VV 
Sbjct: 452 IAEIKRVFHANDNSTMEGIITDAVRECERPPTVSETKRCVGSAEDMIDFATSVLGRSVVL 511

Query: 504 RTTANVNGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRL 563
           RTT +V GS EKV IG V GINGG +T+SVSCHQSLYPYLLYYCHSVPKVR+YE+D+L  
Sbjct: 512 RTTESVAGSKEKVMIGKVNGINGGRVTKSVSCHQSLYPYLLYYCHSVPKVRVYESDLLDP 571

Query: 564 KTKETINRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           K+K  IN G+AICH+DTS+WG  HGAF+ LGS PGQIEVCHWIF+NDM+W++AD
Sbjct: 572 KSKAKINHGIAICHMDTSAWGANHGAFMLLGSRPGQIEVCHWIFENDMNWAIAD 622

BLAST of Sed0009448.1 vs. ExPASy Swiss-Prot
Match: P93217 (Polygalacturonase non-catalytic subunit AroGP2 OS=Solanum lycopersicum OX=4081 GN=GP2 PE=3 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 4.6e-173
Identity = 319/598 (53.34%), Postives = 408/598 (68.23%), Query Frame = 0

Query: 29  PFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGN-LSSRLESFCS 88
           PFT K  +IRYWN H+SN  PKP FL +K+SPL+A   A +    A  N LS+ L+SFCS
Sbjct: 36  PFTPKGYVIRYWNKHVSNDLPKPWFLLNKASPLNAAQYATYTKLVADQNALSTHLQSFCS 95

Query: 89  SANLFCSFDSESRSKAVAAD--FKAYGNRNFVNYGAARLG-GADSFKNYSTGFNSADHSF 148
           SANL C+ D     +    D  F  YGN+NF NYG    G G ++FKNYS   +++ +SF
Sbjct: 96  SANLMCAPDLLPSLEKHTGDIHFTTYGNKNFTNYGTNEPGIGVNTFKNYSE--DASVNSF 155

Query: 149 KRYSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYD 208
           +RY G+ +     F NYA DGNV    F SY T++ GGSG+F++Y    NVP+LRFTAY 
Sbjct: 156 RRY-GRGSPRDNKFDNYAPDGNVIDQSFNSYSTNTPGGSGQFTNYAPNTNVPDLRFTAYS 215

Query: 209 LNARTHKLSFTSYGNETNSGSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGK 268
                 +  F +Y  + NSG QSF SY KNGNG   +F+SY +  N+  STF  YG+   
Sbjct: 216 DQGTGGEQEFKTYLEQGNSGGQSFKSYGKNGNGADSKFTSYGNETNVAASTFKNYGQNAN 275

Query: 269 SANDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTV 328
             N +F +Y  + NNP  NFK+YG GG    +TF+NYR+ +NVG+D F +Y K++++G  
Sbjct: 276 GENQNFTSYSTNGNNPQNNFKNYGVGGNGPSETFTNYRDESNVGDDKFSNYVKDANAGEA 335

Query: 329 NFANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTT-- 388
           NF NYG+SFN G D F  YG+G      + FK+Y ++  F  Y ++  +FS Y N T+  
Sbjct: 336 NFTNYGQSFNEGTDVFITYGKGG-NDPHINFKTYGVNNTFKDYVKDTATFSNYHNKTSQD 395

Query: 389 ------ATGSGIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPF 448
                   G     N+++EPGKFFRE  LK G +M MPDI+DKMP RSFLPR+I   LPF
Sbjct: 396 LASLSEVNGGKKVNNRWIEPGKFFREKMLKSGTIMPMPDIKDKMPKRSFLPRAIAAKLPF 455

Query: 449 SSSSLNETKQIFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGR 508
           S+S ++E K+IFHA + S +  +I +AL+ECERAPS GETK+CV SAEDMIDFAT+VLGR
Sbjct: 456 STSKIDELKKIFHAANDSQVAKMIGDALSECERAPSPGETKQCVNSAEDMIDFATSVLGR 515

Query: 509 NVVARTTANVNGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEAD 568
           NVV RTT N NGS   + IG++KGINGG +T+SVSCHQ+LYP LLYYCHSVPKVR+YEAD
Sbjct: 516 NVVVRTTENTNGSKGNIMIGSIKGINGGKVTKSVSCHQTLYPSLLYYCHSVPKVRVYEAD 575

Query: 569 ILRLKTKETINRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           IL   +K  IN GVAICH+DTSSWGP HGAF+ALGSGPG+IEVCHWIF+NDM+W+ AD
Sbjct: 576 ILDPNSKAKINHGVAICHVDTSSWGPRHGAFIALGSGPGKIEVCHWIFENDMTWATAD 629

BLAST of Sed0009448.1 vs. ExPASy Swiss-Prot
Match: P93218 (Polygalacturonase non-catalytic subunit AroGP3 OS=Solanum lycopersicum OX=4081 GN=GP3 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 1.2e-170
Identity = 315/599 (52.59%), Postives = 407/599 (67.95%), Query Frame = 0

Query: 29  PFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGN-LSSRLESFCS 88
           PFT K  LIRYWN H+SN  PKP FL +K+SPL+A   A +    A  N  ++ L+SFCS
Sbjct: 36  PFTPKGYLIRYWNKHVSNDLPKPWFLLNKASPLNAAQYATYTKLVADQNAFTTHLQSFCS 95

Query: 89  SANLFCSFDSESRSKAVAAD--FKAYGNRNFVNYGAARLG-GADSFKNYSTGFNSADHSF 148
           SANL C+ D     +    D  F +YG++NF NYG    G G +SFKNY+   N   +SF
Sbjct: 96  SANLMCALDLLPSLEKHKGDVHFVSYGDKNFTNYGTKESGLGFNSFKNYTEEENFPVNSF 155

Query: 149 KRYSGKSNNHGEGFTNY-AEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAY 208
           +RY G+ + H   F NY A  GN     F SY T++ GGSG+F++Y    NVP+L FT+Y
Sbjct: 156 RRY-GRDSPHDNQFDNYGAPGGNTPVQSFNSYSTNTPGGSGQFTNYAPGSNVPDLHFTSY 215

Query: 209 DLNARTHKLSFTSYGNETNSGSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAG 268
             +    +  F SYGN+ N+G QSF SY K+GNG   +F++Y +  N+  S FT YGEA 
Sbjct: 216 SDHGTGGEQDFKSYGNDGNAGQQSFKSYGKDGNGADSQFTTYGNNTNVDGSDFTNYGEAA 275

Query: 269 KSANDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGT 328
              N +F +YG + NNP ++F +YG GG    + F++YR+ +NVG+D F +Y K+++ G 
Sbjct: 276 NGENQNFTSYGFNGNNPASSFNNYGVGGNGPSEAFNSYRDQSNVGDDTFTTYVKDANGGE 335

Query: 329 VNFANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTT- 388
            NF NYG+SFN G D F  YG+G      + FK+Y ++  F  Y ++  +FS Y N T+ 
Sbjct: 336 ANFTNYGQSFNEGTDVFTTYGKGG-NDPHINFKTYGVNNTFKDYVKDTATFSNYHNKTSQ 395

Query: 389 -------ATGSGIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLP 448
                    G     N+++EPGKFFRE  LK G +M MPDI+DKMP RSFLPR+I   LP
Sbjct: 396 DLASLSEVNGGKKVNNRWIEPGKFFREKMLKSGTIMPMPDIKDKMPKRSFLPRAIAAKLP 455

Query: 449 FSSSSLNETKQIFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLG 508
           FS+S ++E K+IFHA + S +  +I +AL+ECERAPS GETK+CV SAEDMIDFAT+VLG
Sbjct: 456 FSTSKIDELKKIFHAANDSQVAKMIGDALSECERAPSPGETKQCVNSAEDMIDFATSVLG 515

Query: 509 RNVVARTTANVNGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEA 568
           RNVV RTT N NGS   + IG++KGINGG +T+SVSCHQ+LYP LLYYCHSVPKVR+YEA
Sbjct: 516 RNVVVRTTENTNGSKGNIMIGSIKGINGGKVTKSVSCHQTLYPSLLYYCHSVPKVRVYEA 575

Query: 569 DILRLKTKETINRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           DIL   +K  IN GVAICH+DTSSWGP HGAFVALGSGPG+IEVCHWIF+NDM+W+ AD
Sbjct: 576 DILDPNSKAKINHGVAICHVDTSSWGPRHGAFVALGSGPGKIEVCHWIFENDMTWATAD 632

BLAST of Sed0009448.1 vs. ExPASy TrEMBL
Match: A0A6J1E242 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111430079 PE=4 SV=1)

HSP 1 Score: 995.0 bits (2571), Expect = 1.4e-286
Identity = 495/588 (84.18%), Postives = 545/588 (92.69%), Query Frame = 0

Query: 29  PFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSS 88
           PFTAKASL+RYWN+H+SNQ PKPDFLF+K+SPLSAVDAAVFA  AA G+L+SRLE+FC S
Sbjct: 43  PFTAKASLVRYWNTHVSNQLPKPDFLFAKASPLSAVDAAVFAKHAALGSLTSRLETFCYS 102

Query: 89  ANLFCSFDSESRS--KAVAADFKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFKR 148
           ANLFCSFDS+  S  +   ADFK YGN+NF NYGAAR+GGADSFKNYS G N+ADHSFKR
Sbjct: 103 ANLFCSFDSQETSVPRFKDADFKLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKR 162

Query: 149 YSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDLN 208
           Y G S+NH EGFTNYAEDGNVA ++F+SY  +SKGGSGEF +Y+K+VNVPNLRFTAYDL+
Sbjct: 163 YGGNSSNHKEGFTNYAEDGNVANNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLD 222

Query: 209 ARTHKLSFTSYGNETNSGSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKSA 268
           +R HKLSFTSYGNETNSGSQSFTSY KNGNG PQEFSSYSDG+NILTSTFTGYGE+GKSA
Sbjct: 223 SRAHKLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSA 282

Query: 269 NDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVNF 328
           NDSFKAYGLS NNPH+NFK+YG GG SQIDTFSNYRNGANVG+D+FQSYAKNS+SG VNF
Sbjct: 283 NDSFKAYGLSGNNPHSNFKNYGKGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNF 342

Query: 329 ANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTTATGS 388
           A+YG+SFNHGNDSFKEYG+GSIG+TTVGF+SYS+DRNFTTYAQ GVSFSEYS    ATGS
Sbjct: 343 ASYGQSFNHGNDSFKEYGKGSIGQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGS 402

Query: 389 GIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLNETKQ 448
           GI+VN++VEPGKFFRE ELK+GNVMVMPDIRDKMPPRSFLPR+ILVN+PFSSS ++ETKQ
Sbjct: 403 GINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQ 462

Query: 449 IFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVARTTANV 508
           IFHAR+GSTMEHVIT+ALAECERAPS GETKRCVGSAEDMIDFATAVLGRNVV RTTA+V
Sbjct: 463 IFHARNGSTMEHVITSALAECERAPSMGETKRCVGSAEDMIDFATAVLGRNVVVRTTASV 522

Query: 509 NGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKETI 568
           NGS EKVEIG VKGINGG+LTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT E I
Sbjct: 523 NGSKEKVEIGEVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKI 582

Query: 569 NRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           NRGVAICH+DTSSWGPEHGAFVALGSGPGQIEVCHWIF+NDMSWSVAD
Sbjct: 583 NRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of Sed0009448.1 vs. ExPASy TrEMBL
Match: A0A0A0LP63 (Polygalacturonase-1 non-catalytic subunit beta OS=Cucumis sativus OX=3659 GN=Csa_1G005490 PE=4 SV=1)

HSP 1 Score: 993.8 bits (2568), Expect = 3.2e-286
Identity = 499/631 (79.08%), Postives = 552/631 (87.48%), Query Frame = 0

Query: 1   MAHFVSNANKQIPFSSLFLFLLINPLIF--------------PFTAKASLIRYWNSHISN 60
           ++ F    NKQI F  LFLFLL  P IF              PFT KASL+RYW +H+S 
Sbjct: 17  LSFFHLTTNKQISFKFLFLFLL--PFIFFLSFFPQISSASSNPFTPKASLVRYWKTHVST 76

Query: 61  QFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSSANLFCSFDSESRS---KA 120
             P PDF  SK+SPLSAVDAA  A  AA  +LSSRLESFC SANLFCSFDS+S +   ++
Sbjct: 77  DLPTPDFFLSKASPLSAVDAAALATHAAGASLSSRLESFCFSANLFCSFDSQSETSVPRS 136

Query: 121 VAADFKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFKRYSGKSNNHGEGFTNYAE 180
             ADFK YGN+NFVNYG +RLGG DSFKNYS G N+A+HSFKRY G S+NH EGFT+YAE
Sbjct: 137 KDADFKLYGNKNFVNYGGSRLGGTDSFKNYSDGLNTAEHSFKRYGGNSSNHKEGFTSYAE 196

Query: 181 DGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDLNARTHKLSFTSYGNETNS 240
           DGNVA S+FVSYG  SK GSGEF++Y+KLVNVPNLRFTAYDLN+R+HKLSFTSYGNETNS
Sbjct: 197 DGNVANSNFVSYGAGSKSGSGEFTNYDKLVNVPNLRFTAYDLNSRSHKLSFTSYGNETNS 256

Query: 241 GSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKSANDSFKAYGLSANNPHTN 300
           GSQSFTSY KNGNG PQ+FSSYSDG+NILTSTFTGYGE+GK+ANDSFK YG+S NNPHTN
Sbjct: 257 GSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGESGKTANDSFKEYGISGNNPHTN 316

Query: 301 FKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVNFANYGKSFNHGNDSFKEY 360
           FK+YG GG S+IDTFSNYRNGANVG+D+FQSYAKNS+SG VNFANYGKSFNHGNDSF EY
Sbjct: 317 FKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEY 376

Query: 361 GRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTTATGSGIHVNKYVEPGKFFREL 420
           G+GS+GKTTVGFKSYS+DRNFTTYA+NGVSFS Y+NFTTATGSGIH N +VEPGKFFRE 
Sbjct: 377 GKGSMGKTTVGFKSYSVDRNFTTYAKNGVSFSVYNNFTTATGSGIHANSHVEPGKFFRES 436

Query: 421 ELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLNETKQIFHARDGSTMEHVITNA 480
           ELK+GNVMVMPDI+DKMPPRSFLPRSILVNLPFSSS ++E K++FHARDGSTME VITNA
Sbjct: 437 ELKQGNVMVMPDIKDKMPPRSFLPRSILVNLPFSSSRISEAKEMFHARDGSTMESVITNA 496

Query: 481 LAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSAEKVEIGTVKGING 540
           LAECERAPS+GETKRCVGSAEDMIDFAT VLG+ VV RTTANVNGS EK+EIG VKGING
Sbjct: 497 LAECERAPSRGETKRCVGSAEDMIDFATTVLGQRVVVRTTANVNGSKEKIEIGRVKGING 556

Query: 541 GDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKETINRGVAICHIDTSSWGPE 600
           GDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADIL+++TKE INRGVAICHIDTSSWGPE
Sbjct: 557 GDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKMETKEKINRGVAICHIDTSSWGPE 616

Query: 601 HGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           HGAFVALGSGPGQIEVCHWIF+NDMSW+VAD
Sbjct: 617 HGAFVALGSGPGQIEVCHWIFENDMSWNVAD 645

BLAST of Sed0009448.1 vs. ExPASy TrEMBL
Match: A0A1S3BVD2 (polygalacturonase 1 beta-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103493915 PE=4 SV=1)

HSP 1 Score: 990.7 bits (2560), Expect = 2.7e-285
Identity = 501/632 (79.27%), Postives = 549/632 (86.87%), Query Frame = 0

Query: 1   MAHFVSNANKQIPFSSLFLFLLINPLIF---------------PFTAKASLIRYWNSHIS 60
           ++ F    NKQI F  LFLFLL  P  F               PFT KASL+RYW +H+S
Sbjct: 19  LSFFHLTTNKQISFKVLFLFLL--PFFFFLSFFPQISSAASSNPFTPKASLVRYWKTHVS 78

Query: 61  NQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSSANLFCSFDSESR---SK 120
              P PDF  SK+SPLS+VDAAV A  AA  +LSSRLESFC SANLFCSFDS+S     +
Sbjct: 79  TDLPTPDFFLSKASPLSSVDAAVLATHAAGASLSSRLESFCYSANLFCSFDSQSEISVPR 138

Query: 121 AVAADFKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFKRYSGKSNNHGEGFTNYA 180
              ADFK YGN+NF NYG  RLGG DSFKNYS G N+A+HSFKRY G S+NH EGFT+YA
Sbjct: 139 FKDADFKLYGNKNFANYGGNRLGGTDSFKNYSDGLNTAEHSFKRYGGNSSNHKEGFTSYA 198

Query: 181 EDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDLNARTHKLSFTSYGNETN 240
           EDGNVA S+FVSYG +SK GSG F++Y+KLVNVPNLRFTAYDLN+R+HKL+FTSYGNETN
Sbjct: 199 EDGNVANSNFVSYGAESKSGSGGFTNYDKLVNVPNLRFTAYDLNSRSHKLTFTSYGNETN 258

Query: 241 SGSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKSANDSFKAYGLSANNPHT 300
           SGSQSFTSY KNGNG PQ+FSSYSDG+NILTSTFTGYGE+GK+ANDSFK YG+S NNPHT
Sbjct: 259 SGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGESGKTANDSFKEYGVSGNNPHT 318

Query: 301 NFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVNFANYGKSFNHGNDSFKE 360
           NFK+YG GG S+IDTFSNYRNGANVG+D+FQSYAKNS+SG VNFANYGKSFNHGNDSF E
Sbjct: 319 NFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTE 378

Query: 361 YGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTTATGSGIHVNKYVEPGKFFRE 420
           YG+GSIGKTTVGFKSYS+DRNFTTYA+NGVSFSEYSNFTTATGSGIHVN +VEPGKFFRE
Sbjct: 379 YGKGSIGKTTVGFKSYSIDRNFTTYAKNGVSFSEYSNFTTATGSGIHVNSHVEPGKFFRE 438

Query: 421 LELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLNETKQIFHARDGSTMEHVITN 480
            ELK+GNVMVMPDIRDKMPPRSFLPR+ILVNLPFSSS ++E K++FHARDGSTME VITN
Sbjct: 439 SELKQGNVMVMPDIRDKMPPRSFLPRAILVNLPFSSSRISEAKEMFHARDGSTMESVITN 498

Query: 481 ALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSAEKVEIGTVKGIN 540
           ALAECERAPS+GETKRCVGSAEDMIDFAT VLGR VV RTTANVNGS EKVEIG VKGIN
Sbjct: 499 ALAECERAPSRGETKRCVGSAEDMIDFATTVLGRRVVVRTTANVNGSKEKVEIGRVKGIN 558

Query: 541 GGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKETINRGVAICHIDTSSWGP 600
           GG+LTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILR++TKE INRGVAICHIDTSSWGP
Sbjct: 559 GGNLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRVETKEKINRGVAICHIDTSSWGP 618

Query: 601 EHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           EHGAFVALGS PGQIEVCHWIF+NDMSWSVAD
Sbjct: 619 EHGAFVALGSSPGQIEVCHWIFENDMSWSVAD 648

BLAST of Sed0009448.1 vs. ExPASy TrEMBL
Match: A0A6J1JM21 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111485620 PE=4 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 7.8e-285
Identity = 493/588 (83.84%), Postives = 544/588 (92.52%), Query Frame = 0

Query: 29  PFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSS 88
           PFTAKASL+RYWN+H+SNQ PKPDFLF K+SPLSAVDAAVFA  AA G+L+SRLE+FC S
Sbjct: 43  PFTAKASLVRYWNTHVSNQLPKPDFLFVKASPLSAVDAAVFAKHAALGSLTSRLETFCYS 102

Query: 89  ANLFCSFDSESRS--KAVAADFKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFKR 148
           ANLFCSFDS+  S  +   ADFK YGN+NF NYGAAR+GGADSFKNYS G N+ADHSFKR
Sbjct: 103 ANLFCSFDSQETSVPRFKDADFKLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKR 162

Query: 149 YSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDLN 208
           Y G S+NH EGFTNYAEDGNVA ++F+SY  +SKGGSGEF +Y+K+VNVPNLRFTAYDL+
Sbjct: 163 YGGNSSNHKEGFTNYAEDGNVANNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLD 222

Query: 209 ARTHKLSFTSYGNETNSGSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKSA 268
           +R HKLSFTSYGNETNSGSQSFTSY KNGNG PQEFSSYSDG+NILTSTFTGYGE+GKSA
Sbjct: 223 SRAHKLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSA 282

Query: 269 NDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVNF 328
           NDSFKAYGLS+NNPH+NFK+YG GG SQIDTFSNYRNGANVG+D+FQSYAKNS+SG VNF
Sbjct: 283 NDSFKAYGLSSNNPHSNFKNYGKGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNF 342

Query: 329 ANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTTATGS 388
           A+YG+SFNHGNDSFKEY +GSIG+TTVGF+SYS+DRNFTTYAQ GVSFSEYS    ATGS
Sbjct: 343 ASYGQSFNHGNDSFKEYAKGSIGQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGS 402

Query: 389 GIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLNETKQ 448
           GI+VN++VEPGKFFRE ELK+GNVMVMPDIRDKMPPRSFLPR+ILVN+PFSSS ++ETKQ
Sbjct: 403 GINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQ 462

Query: 449 IFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVARTTANV 508
           IFHAR+GSTME VIT+ALAECERAPS GETKRCVGSAEDMIDFATAVLGRNVVARTTA+V
Sbjct: 463 IFHARNGSTMERVITSALAECERAPSMGETKRCVGSAEDMIDFATAVLGRNVVARTTASV 522

Query: 509 NGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKETI 568
           NGS EKVEIG VKGINGG+LTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT E I
Sbjct: 523 NGSTEKVEIGEVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKI 582

Query: 569 NRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           NRGVAICH+DTSSWGPEHGAFVALGSGPGQIEVCHWIF+ND+SWSVAD
Sbjct: 583 NRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDVSWSVAD 626

BLAST of Sed0009448.1 vs. ExPASy TrEMBL
Match: A0A5D3BIQ0 (Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001790 PE=4 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 1.6e-282
Identity = 487/589 (82.68%), Postives = 533/589 (90.49%), Query Frame = 0

Query: 29  PFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSS 88
           PFT KASL+RYW +H+S   P PDF  SK+SPLS+VDAAV A  AA  +LSSRLESFC S
Sbjct: 10  PFTPKASLVRYWKTHVSTDLPTPDFFLSKASPLSSVDAAVLATHAAGASLSSRLESFCYS 69

Query: 89  ANLFCSFDSESR---SKAVAADFKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFK 148
           ANLFCSFDS+S     +   ADFK YGN+NF NYG  RLGG DSFKNYS G N+A+HSFK
Sbjct: 70  ANLFCSFDSQSEISVPRFKDADFKLYGNKNFANYGGNRLGGTDSFKNYSDGLNTAEHSFK 129

Query: 149 RYSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDL 208
           RY G S+NH EGFT+YAEDGNVA S+FVSYG +SK GSG F++Y+KLVNVPNLRFTAYDL
Sbjct: 130 RYGGNSSNHKEGFTSYAEDGNVANSNFVSYGAESKSGSGGFTNYDKLVNVPNLRFTAYDL 189

Query: 209 NARTHKLSFTSYGNETNSGSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKS 268
           N+R+HKL+FTSYGNETNSGSQSFTSY KNGNG PQ+FSSYSDG+NILTSTFTGYGE+GK+
Sbjct: 190 NSRSHKLTFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGESGKT 249

Query: 269 ANDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVN 328
           ANDSFK YG+S NNPHTNFK+YG GG S+IDTFSNYRNGANVG+D+FQSYAKNS+SG VN
Sbjct: 250 ANDSFKEYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVN 309

Query: 329 FANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTTATG 388
           FANYGKSFNHGNDSF EYG+GSIGKTTVGFKSYS+DRNFTTYA+NGVSFSEYSNFTTATG
Sbjct: 310 FANYGKSFNHGNDSFTEYGKGSIGKTTVGFKSYSIDRNFTTYAKNGVSFSEYSNFTTATG 369

Query: 389 SGIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLNETK 448
           SGIHVN +VEPGKFFRE ELK+GNVMVMPDIRDKMPPRSFLPR+ILVNLPFSSS ++E K
Sbjct: 370 SGIHVNSHVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNLPFSSSRISEAK 429

Query: 449 QIFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVARTTAN 508
           ++FHARDGSTME VITNALAECERAPS+GETKRCVGSAEDMIDFAT VLGR VV RTTAN
Sbjct: 430 EMFHARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMIDFATTVLGRRVVVRTTAN 489

Query: 509 VNGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKET 568
           VNGS EKVEIG VKGINGG+LTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILR++TKE 
Sbjct: 490 VNGSKEKVEIGRVKGINGGNLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRVETKEK 549

Query: 569 INRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           INRGVAICHIDTSSWGPEHGAFVALGS PGQIEVCHWIF+NDMSWSVAD
Sbjct: 550 INRGVAICHIDTSSWGPEHGAFVALGSSPGQIEVCHWIFENDMSWSVAD 598

BLAST of Sed0009448.1 vs. TAIR 10
Match: AT1G70370.1 (polygalacturonase 2 )

HSP 1 Score: 671.8 bits (1732), Expect = 5.3e-193
Identity = 346/592 (58.45%), Postives = 434/592 (73.31%), Query Frame = 0

Query: 29  PFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSS 88
           PFT KASL+RYWN  I  Q P+ +FL SK+SPL+AVD+A F+  AAA +L +R   FCS+
Sbjct: 36  PFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAANSLPTRFPDFCSA 95

Query: 89  ANLFCSFDSESRSKAVAAD--FKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFKR 148
           ANLFC  D  +  +    D  F  Y  +NF NYG AR GGADSFKNYS   N    SF+R
Sbjct: 96  ANLFCFPDLGASLEKHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGNVVTDSFRR 155

Query: 149 YSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDLN 208
           YS  +  H + FT Y E+ NV    F SYGT   GG+G+F++Y+  VN P  RFTAY   
Sbjct: 156 YSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTSRFTAYSDG 215

Query: 209 ARTHKLSFTSYGNETNSGS-QSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKS 268
                 +F +Y +E N+G+ QSFTSY KNGNG P EF+SY   +N++ S F+ YGE+G +
Sbjct: 216 GNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFSNYGESGNA 275

Query: 269 ANDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVN 328
           AND+F +YG   N P  NF +YG  G + +DTF+NYR+ ANVG+D+F SYAK+S+S  VN
Sbjct: 276 ANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAKDSNSEKVN 335

Query: 329 FANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTT--- 388
           F NYG+SFN G+++F  YG+G+ G + + FK+Y+ +  F  YA+ GV+F++Y+  TT   
Sbjct: 336 FVNYGQSFNPGSETFTGYGKGAEG-SKLSFKTYTPNSTFKDYAKKGVAFAKYNVSTTTAN 395

Query: 389 ATGSGIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLN 448
             G G  VNK++EPGKFFRE  LKEG V+ MPDI+DKMP RSFLPRSI+  LPFS+S L 
Sbjct: 396 TVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLPFSTSKLG 455

Query: 449 ETKQIFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVART 508
           E K+IFHA + STM  +IT+A+ ECER PS GETKRCVGSAEDMIDFAT+VLGR+VV RT
Sbjct: 456 EIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLGRSVVLRT 515

Query: 509 TANVNGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT 568
           T NV GS EKV IG V GINGG LT++VSCHQSLYPYLLYYCHSVPKVR+YEAD+L L +
Sbjct: 516 TENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLELNS 575

Query: 569 KETINRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           K+ IN G+AICH+DTSSWGP HGAF+ALGS PG+IEVCHWIF+NDM+W++AD
Sbjct: 576 KKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Sed0009448.1 vs. TAIR 10
Match: AT1G70370.2 (polygalacturonase 2 )

HSP 1 Score: 671.8 bits (1732), Expect = 5.3e-193
Identity = 346/592 (58.45%), Postives = 434/592 (73.31%), Query Frame = 0

Query: 29  PFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLESFCSS 88
           PFT KASL+RYWN  I  Q P+ +FL SK+SPL+AVD+A F+  AAA +L +R   FCS+
Sbjct: 36  PFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAANSLPTRFPDFCSA 95

Query: 89  ANLFCSFDSESRSKAVAAD--FKAYGNRNFVNYGAARLGGADSFKNYSTGFNSADHSFKR 148
           ANLFC  D  +  +    D  F  Y  +NF NYG AR GGADSFKNYS   N    SF+R
Sbjct: 96  ANLFCFPDLGASLEKHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGNVVTDSFRR 155

Query: 149 YSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFTAYDLN 208
           YS  +  H + FT Y E+ NV    F SYGT   GG+G+F++Y+  VN P  RFTAY   
Sbjct: 156 YSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTSRFTAYSDG 215

Query: 209 ARTHKLSFTSYGNETNSGS-QSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYGEAGKS 268
                 +F +Y +E N+G+ QSFTSY KNGNG P EF+SY   +N++ S F+ YGE+G +
Sbjct: 216 GNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFSNYGESGNA 275

Query: 269 ANDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSSSGTVN 328
           AND+F +YG   N P  NF +YG  G + +DTF+NYR+ ANVG+D+F SYAK+S+S  VN
Sbjct: 276 ANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAKDSNSEKVN 335

Query: 329 FANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNFTT--- 388
           F NYG+SFN G+++F  YG+G+ G + + FK+Y+ +  F  YA+ GV+F++Y+  TT   
Sbjct: 336 FVNYGQSFNPGSETFTGYGKGAEG-SKLSFKTYTPNSTFKDYAKKGVAFAKYNVSTTTAN 395

Query: 389 ATGSGIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSSLN 448
             G G  VNK++EPGKFFRE  LKEG V+ MPDI+DKMP RSFLPRSI+  LPFS+S L 
Sbjct: 396 TVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLPFSTSKLG 455

Query: 449 ETKQIFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVART 508
           E K+IFHA + STM  +IT+A+ ECER PS GETKRCVGSAEDMIDFAT+VLGR+VV RT
Sbjct: 456 EIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLGRSVVLRT 515

Query: 509 TANVNGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT 568
           T NV GS EKV IG V GINGG LT++VSCHQSLYPYLLYYCHSVPKVR+YEAD+L L +
Sbjct: 516 TENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLELNS 575

Query: 569 KETINRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           K+ IN G+AICH+DTSSWGP HGAF+ALGS PG+IEVCHWIF+NDM+W++AD
Sbjct: 576 KKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Sed0009448.1 vs. TAIR 10
Match: AT1G60390.1 (polygalacturonase 1 )

HSP 1 Score: 659.4 bits (1700), Expect = 2.7e-189
Identity = 342/595 (57.48%), Postives = 427/595 (71.76%), Query Frame = 0

Query: 23  INPLIFPFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRL 82
           I P   PFT KASLIRYWN+HI+   PKP F  SK+SPL+AVD+  FA+ A+   L++  
Sbjct: 33  ITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPLTAVDSTRFASLASNHALNTHH 92

Query: 83  ESFCSSANLFCSFDSESRSKAVAAD---FKAYGNRNFVNYGAARLGGADSFKNYSTGFNS 142
             FCS+A LFC  +  + S     D   F AY  +NF NYG+ RL GADSFKNYS G N 
Sbjct: 93  SDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTNYGSDRLSGADSFKNYSGGDNI 152

Query: 143 ADHSFKRYSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLR 202
           A  SF+RYS  S  H +GFTNYA + NVA   F +Y T + GGSGEF++Y    N PN R
Sbjct: 153 AVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATGTTGGSGEFTNYNTDANEPNGR 212

Query: 203 FTAYDLNARTHKLSFTSYGNETNSGSQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGY 262
           FT+Y   A     +FT+Y    N+G QSFTSY+KNGNG P EFS Y  G+N++ + FT Y
Sbjct: 213 FTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGAPNEFSGYGTGSNVVNTGFTKY 272

Query: 263 GEAGKSANDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNS 322
           GE+   ANDSF +YG + N P   FK YG+GG   +  F NYR+ +N+G D+F SYAKNS
Sbjct: 273 GESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGFKNYRDQSNIGVDSFSSYAKNS 332

Query: 323 SSGTVNFANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSN 382
           ++  VNF NYGKSFN G+D+F  YG+ ++G   V FK+Y   ++F  Y ++GV F+ YSN
Sbjct: 333 NNEKVNFVNYGKSFNLGSDNFTGYGQDNVG-GNVSFKTYGQGQSFKVYTKDGVVFARYSN 392

Query: 383 FTTATGSGIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSS 442
               + +G  VNK+VE GKFFRE  LKEG +M MPDI+DKMP R+FLPR+I+ NLPFSSS
Sbjct: 393 --NVSSNGKTVNKWVEEGKFFREAMLKEGTLMQMPDIKDKMPKRTFLPRNIVKNLPFSSS 452

Query: 443 SLNETKQIFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVV 502
           ++ E  ++F A + S+M  +I++A++ECER  S GETKRCVGSAEDMIDFAT+VLGR VV
Sbjct: 453 TIGEIWRVFGAGENSSMAGIISSAVSECERPASHGETKRCVGSAEDMIDFATSVLGRGVV 512

Query: 503 ARTTANVNGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILR 562
            RTT NV GS +KV IG V GINGGD+TR+VSCHQSLYPYLLYYCHSVP+VR+YE D+L 
Sbjct: 513 VRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQSLYPYLLYYCHSVPRVRVYETDLLD 572

Query: 563 LKTKETINRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
            K+ E IN GVAICHIDTS+W P HGAF+ALGSGPGQIEVCHWIF+NDM+W++ D
Sbjct: 573 PKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPGQIEVCHWIFENDMTWNIID 624

BLAST of Sed0009448.1 vs. TAIR 10
Match: AT1G23760.1 (BURP domain-containing protein )

HSP 1 Score: 651.0 bits (1678), Expect = 9.7e-187
Identity = 341/594 (57.41%), Postives = 421/594 (70.88%), Query Frame = 0

Query: 24  NPLIFPFTAKASLIRYWNSHISNQFPKPDFLFSKSSPLSAVDAAVFAAAAAAGNLSSRLE 83
           +P I PFT KASL+RYWN  I +Q P   FL SK+SPL+AV +A FA  A+   L  RL 
Sbjct: 32  SPEINPFTPKASLVRYWNKQIHHQSPGSAFLLSKASPLTAVASATFAKLASENALPDRLP 91

Query: 84  SFCSSANLFCSFDSESRSKAVAAD--FKAYGNRNFVNYGAARLGGADSFKNYSTGFNSAD 143
            FCS+ANLFC  D     +  + D  F  Y  RNF NYG    GGADSFK YS   N+  
Sbjct: 92  DFCSAANLFCFPDLGPTLEKHSNDVKFSVYDQRNFTNYGTIHPGGADSFKKYSQNGNAVT 151

Query: 144 HSFKRYSGKSNNHGEGFTNYAEDGNVATSDFVSYGTDSKGGSGEFSSYEKLVNVPNLRFT 203
            SF+RYS  +  H + FT YA++ NV    F SYGT + GGSG+F +Y+  VN P  RF 
Sbjct: 152 DSFRRYSRDAAGHEDKFTGYADNSNVVEEKFNSYGTSATGGSGDFKNYQSGVNNPTTRFK 211

Query: 204 AYDLNARTHKLSFTSYGNETNSG-SQSFTSYAKNGNGDPQEFSSYSDGANILTSTFTGYG 263
           AY         +F +Y +E NSG  QSFTSY KNGNG P EFSSY   +N++ S F+ YG
Sbjct: 212 AYSDEGNGRAQTFKTYTHEANSGPGQSFTSYGKNGNGAPNEFSSYGVSSNVIGSDFSNYG 271

Query: 264 EAGKSANDSFKAYGLSANNPHTNFKSYGNGGTSQIDTFSNYRNGANVGEDNFQSYAKNSS 323
           E G +AND+FK YG   N P  NFKSYG  G + +DTF NYR+ ANVG+D+F SYAKNS+
Sbjct: 272 ENGNAANDTFKGYGGDGNVPQNNFKSYGASGNAAVDTFLNYRDNANVGDDSFSSYAKNSN 331

Query: 324 SGTVNFANYGKSFNHGNDSFKEYGRGSIGKTTVGFKSYSMDRNFTTYAQNGVSFSEYSNF 383
              V+F NYGKS N G++SF  YG+G+ G   + FK+Y+ +  F  Y + GV F++Y+  
Sbjct: 332 FEKVDFVNYGKSINPGSESFSGYGKGAEG-NKIDFKTYTQNSTFKDYTKTGVEFAKYNR- 391

Query: 384 TTATGSGIHVNKYVEPGKFFRELELKEGNVMVMPDIRDKMPPRSFLPRSILVNLPFSSSS 443
            ++ G G  VNK+VEPGKFFRE  LKEG ++ MPDI+DKMP RSFLPRSI+  LPFS+S 
Sbjct: 392 -SSLGGGKTVNKWVEPGKFFRESMLKEGTLIWMPDIKDKMPKRSFLPRSIVSKLPFSTSK 451

Query: 444 LNETKQIFHARDGSTMEHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGRNVVA 503
           + E K++FHA D STME +IT+A+ ECER P+  ETKRCVGSAEDMIDFAT+VLGR+VV 
Sbjct: 452 IAEIKRVFHANDNSTMEGIITDAVRECERPPTVSETKRCVGSAEDMIDFATSVLGRSVVL 511

Query: 504 RTTANVNGSAEKVEIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRL 563
           RTT +V GS EKV IG V GINGG +T+SVSCHQSLYPYLLYYCHSVPKVR+YE+D+L  
Sbjct: 512 RTTESVAGSKEKVMIGKVNGINGGRVTKSVSCHQSLYPYLLYYCHSVPKVRVYESDLLDP 571

Query: 564 KTKETINRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSWSVAD 615
           K+K  IN G+AICH+DTS+WG  HGAF+ LGS PGQIEVCHWIF+NDM+W++AD
Sbjct: 572 KSKAKINHGIAICHMDTSAWGANHGAFMLLGSRPGQIEVCHWIFENDMNWAIAD 622

BLAST of Sed0009448.1 vs. TAIR 10
Match: AT5G25610.1 (BURP domain-containing protein )

HSP 1 Score: 113.6 bits (283), Expect = 5.6e-25
Identity = 68/217 (31.34%), Postives = 102/217 (47.00%), Query Frame = 0

Query: 399 FFRELELKEGNVM-VMPDIRDKMPPR-SFLPRSILVNLPFSSSSLNETKQIFHARDGSTM 458
           FF E +L  G  M V  +  D    + +FLPR     +PF S   +ET + F    GS  
Sbjct: 176 FFLEKDLVRGKEMNVRFNAEDGYGGKTAFLPRGEAETVPFGSEKFSETLKRFSVEAGSEE 235

Query: 459 EHVITNALAECERAPSQGETKRCVGSAEDMIDFATAVLGR---NVVARTTANVNGSAEKV 518
             ++   + ECE     GE K C  S E M+DF+ + LG+     V+   A  N   +K 
Sbjct: 236 AEMMKKTIEECEARKVSGEEKYCATSLESMVDFSVSKLGKYHVRAVSTEVAKKNAPMQKY 295

Query: 519 EIGTVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKETINRGVAIC 578
           +I    G+      +SV CH+  YP+ ++YCH      +Y   +          + VA+C
Sbjct: 296 KIAAA-GVKKLSDDKSVVCHKQKYPFAVFYCHKAMMTTVYAVPLEGENGMRA--KAVAVC 355

Query: 579 HIDTSSWGPEHGAFVALGSGPGQIEVCHWIFQNDMSW 611
           H +TS+W P H AF  L   PG + VCH++ +  + W
Sbjct: 356 HKNTSAWNPNHLAFKVLKVKPGTVPVCHFLPETHVVW 389

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878610.14.4e-29085.57polygalacturonase 1 beta-like protein 3 [Benincasa hispida][more]
XP_022921994.12.9e-28684.18polygalacturonase 1 beta-like protein 3 [Cucurbita moschata][more]
KAG6589452.13.8e-28684.18Polygalacturonase 1 beta-like protein 3, partial [Cucurbita argyrosperma subsp. ... [more]
XP_004138283.16.5e-28679.08polygalacturonase 1 beta-like protein 3 [Cucumis sativus] >KGN63588.1 hypothetic... [more]
XP_023516985.11.9e-28583.84polygalacturonase 1 beta-like protein 3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P929907.5e-19258.45Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 ... [more]
O807603.8e-18857.48Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 ... [more]
P929821.4e-18557.41Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 ... [more]
P932174.6e-17353.34Polygalacturonase non-catalytic subunit AroGP2 OS=Solanum lycopersicum OX=4081 G... [more]
P932181.2e-17052.59Polygalacturonase non-catalytic subunit AroGP3 OS=Solanum lycopersicum OX=4081 G... [more]
Match NameE-valueIdentityDescription
A0A6J1E2421.4e-28684.18polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A0A0LP633.2e-28679.08Polygalacturonase-1 non-catalytic subunit beta OS=Cucumis sativus OX=3659 GN=Csa... [more]
A0A1S3BVD22.7e-28579.27polygalacturonase 1 beta-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103493915 ... [more]
A0A6J1JM217.8e-28583.84polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111485... [more]
A0A5D3BIQ01.6e-28282.68Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
AT1G70370.15.3e-19358.45polygalacturonase 2 [more]
AT1G70370.25.3e-19358.45polygalacturonase 2 [more]
AT1G60390.12.7e-18957.48polygalacturonase 1 [more]
AT1G23760.19.7e-18757.41BURP domain-containing protein [more]
AT5G25610.15.6e-2531.34BURP domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004873BURP domainSMARTSM01045BURP_2coord: 397..613
e-value: 7.8E-120
score: 414.1
IPR004873BURP domainPFAMPF03181BURPcoord: 399..610
e-value: 5.6E-62
score: 209.1
IPR004873BURP domainPROSITEPS51277BURPcoord: 399..613
score: 62.48381
NoneNo IPR availablePANTHERPTHR31458POLYGALACTURONASE 1 BETA-LIKE PROTEIN 2coord: 28..614
NoneNo IPR availablePANTHERPTHR31458:SF9POLYGALACTURONASE-1 NON-CATALYTIC SUBUNIT BETAcoord: 28..614

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Sed0009448Sed0009448gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0009448.1-exonSed0009448.1-exon-LG08:757274..757356exon
Sed0009448.1-exonSed0009448.1-exon-LG08:757405..759166exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0009448.1-cdsSed0009448.1-cds-LG08:757274..757356CDS
Sed0009448.1-cdsSed0009448.1-cds-LG08:757405..759166CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Sed0009448.1Sed0009448.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane