Sed0008584.1 (mRNA) Chayote v1

Overview
NameSed0008584.1
TypemRNA
OrganismSechium edule (Chayote v1)
DescriptionrRNA N-glycosidase
LocationLG09: 4853857 .. 4854935 (-)
Sequence length1079
RNA-Seq ExpressionSed0008584.1
SyntenySed0008584.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATAAACACAGCACCATTGTTCTCACAATTCAAAAACAAAAGAAAAATATGAAATCAATTGGATTTTACTTTGTTGTGGCTCTCTATTTTGGTGCTCATTTTACGGATGCTGATATCAACTTCAATCTATTGGGTGGCACTGGCGCAACCTACAAGCAATTCATCAAAAACTTACGCACAACGCTCACCGTTGGATCTCCGATAGTCGAGGGCATACCCGTGTTGAAGGCCTCGGCATCAGGCTTGGCGCGCTTCCTACTAGTTCACCTCGCGAATTACAACGGCGAATCGATTAGCGTCGCACTAGACGTCGTTAACGTGTACGTCGTAGCCTATCGAGCTGGAAACACTGCCTACTTTCTCAACGACGCGTCAGCAGAAGCCAACCGAGTGCTATTTCAAGGCTTCGCTCATGTAACACTCCCCTACGGGGGAAATTACGATGGCCTCGAGACCGCAGCGGGGAGAATTTCGAGGGAGAAAATTTACCTTGGATTTTCTGAAATGAGTAGTGCTATTGGGAGCATGTTCCATTACACTACTGGGACTTCGGTACCTAAAGCGTTTATCGTGATCATTCAATCGGTCTCGGAAGCTGCGAGATTTAAATATATCGAGCAAAGGGTTTCTGAAAATGTTGAGACGAAGTTTCTTCCTGATCCTGCCTTTTTAAGCTTGCAAAATAGGTGGAGCGATCTCTCTGAACAAATACAGATTGCGCAGACTCGAGGCGGGAAGTTTGCTCGTCCCGTCGAGGTTCGCAGTGTCAGCAACACTCCGATCATTGTCTCCGATGTTAGTTCGCCCGTTGTCAAAGGCATTGCGCTTCTATTGTACTATAAAGTTAATGTTGGCACTGATAATGTTGTTGTCAAGGTACTAGTTACTCATCAAACTACTATGTGGTAGTATATCTGCAAGTATTAAAATAAAGGGTTAATCTTAAATTAACATGACAATTTCAATTTTTTGTTTTTGGTTTTCTATTTTTTAGTTTTCTGTTTCTAAAAAATAAAATCTAAATTTTTCTATATTTTTTAATTTCAGTATTTTTTAGTTTGCATAATTAGAGATGGAAA

mRNA sequence

TATAAACACAGCACCATTGTTCTCACAATTCAAAAACAAAAGAAAAATATGAAATCAATTGGATTTTACTTTGTTGTGGCTCTCTATTTTGGTGCTCATTTTACGGATGCTGATATCAACTTCAATCTATTGGGTGGCACTGGCGCAACCTACAAGCAATTCATCAAAAACTTACGCACAACGCTCACCGTTGGATCTCCGATAGTCGAGGGCATACCCGTGTTGAAGGCCTCGGCATCAGGCTTGGCGCGCTTCCTACTAGTTCACCTCGCGAATTACAACGGCGAATCGATTAGCGTCGCACTAGACGTCGTTAACGTGTACGTCGTAGCCTATCGAGCTGGAAACACTGCCTACTTTCTCAACGACGCGTCAGCAGAAGCCAACCGAGTGCTATTTCAAGGCTTCGCTCATGTAACACTCCCCTACGGGGGAAATTACGATGGCCTCGAGACCGCAGCGGGGAGAATTTCGAGGGAGAAAATTTACCTTGGATTTTCTGAAATGAGTAGTGCTATTGGGAGCATGTTCCATTACACTACTGGGACTTCGGTACCTAAAGCGTTTATCGTGATCATTCAATCGGTCTCGGAAGCTGCGAGATTTAAATATATCGAGCAAAGGGTTTCTGAAAATGTTGAGACGAAGTTTCTTCCTGATCCTGCCTTTTTAAGCTTGCAAAATAGGTGGAGCGATCTCTCTGAACAAATACAGATTGCGCAGACTCGAGGCGGGAAGTTTGCTCGTCCCGTCGAGGTTCGCAGTGTCAGCAACACTCCGATCATTGTCTCCGATGTTAGTTCGCCCGTTGTCAAAGGCATTGCGCTTCTATTGTACTATAAAGTTAATGTTGGCACTGATAATGTTGTTGTCAAGGTACTAGTTACTCATCAAACTACTATGTGGTAGTATATCTGCAAGTATTAAAATAAAGGGTTAATCTTAAATTAACATGACAATTTCAATTTTTTGTTTTTGGTTTTCTATTTTTTAGTTTTCTGTTTCTAAAAAATAAAATCTAAATTTTTCTATATTTTTTAATTTCAGTATTTTTTAGTTTGCATAATTAGAGATGGAAA

Coding sequence (CDS)

ATGAAATCAATTGGATTTTACTTTGTTGTGGCTCTCTATTTTGGTGCTCATTTTACGGATGCTGATATCAACTTCAATCTATTGGGTGGCACTGGCGCAACCTACAAGCAATTCATCAAAAACTTACGCACAACGCTCACCGTTGGATCTCCGATAGTCGAGGGCATACCCGTGTTGAAGGCCTCGGCATCAGGCTTGGCGCGCTTCCTACTAGTTCACCTCGCGAATTACAACGGCGAATCGATTAGCGTCGCACTAGACGTCGTTAACGTGTACGTCGTAGCCTATCGAGCTGGAAACACTGCCTACTTTCTCAACGACGCGTCAGCAGAAGCCAACCGAGTGCTATTTCAAGGCTTCGCTCATGTAACACTCCCCTACGGGGGAAATTACGATGGCCTCGAGACCGCAGCGGGGAGAATTTCGAGGGAGAAAATTTACCTTGGATTTTCTGAAATGAGTAGTGCTATTGGGAGCATGTTCCATTACACTACTGGGACTTCGGTACCTAAAGCGTTTATCGTGATCATTCAATCGGTCTCGGAAGCTGCGAGATTTAAATATATCGAGCAAAGGGTTTCTGAAAATGTTGAGACGAAGTTTCTTCCTGATCCTGCCTTTTTAAGCTTGCAAAATAGGTGGAGCGATCTCTCTGAACAAATACAGATTGCGCAGACTCGAGGCGGGAAGTTTGCTCGTCCCGTCGAGGTTCGCAGTGTCAGCAACACTCCGATCATTGTCTCCGATGTTAGTTCGCCCGTTGTCAAAGGCATTGCGCTTCTATTGTACTATAAAGTTAATGTTGGCACTGATAATGTTGTTGTCAAGGTACTAGTTACTCATCAAACTACTATGTGGTAG

Protein sequence

MKSIGFYFVVALYFGAHFTDADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLKASASGLARFLLVHLANYNGESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQGFAHVTLPYGGNYDGLETAAGRISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSVSEAARFKYIEQRVSENVETKFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSVSNTPIIVSDVSSPVVKGIALLLYYKVNVGTDNVVVKVLVTHQTTMW
Homology
BLAST of Sed0008584.1 vs. NCBI nr
Match: BAG12408.1 (karasurin-H precursor [Trichosanthes kirilowii var. japonica])

HSP 1 Score: 442.6 bits (1137), Expect = 2.6e-120
Identity = 224/281 (79.72%), Postives = 250/281 (88.97%), Query Frame = 0

Query: 1   MKSIGFYFVVALYFGAHFTDADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLK 60
           MK +GFY V+ALY GA  T+AD+NF+LLG T ATYKQFI+NLR  LTVGSP VE IPVLK
Sbjct: 1   MKLVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLK 60

Query: 61  ASASGLARFLLVHLANYNGESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQGF 120
            +A+GLARFLLVHL NYNGESI+VA+DVVNVYVVAYRAGN AYFLNDASAEAN VLF+G 
Sbjct: 61  GTAAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 AHVTLPYGGNYDGLETAAGRISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSV 180
            HV LPYGGNYDGLETAAGRISREKIYLGFSE+SSAIGSMFHY  GTSVPKAFIVIIQ+V
Sbjct: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTV 180

Query: 181 SEAARFKYIEQRVSENVETKFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSV 240
           SEAARFKYI+QRVSENV+TKFLPDPAFLSL+N W  LSEQIQIAQ RGG+FARP+E+R+V
Sbjct: 181 SEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTV 240

Query: 241 SNTPIIVSDVSSPVVKGIALLLYYKVNVGTDNVVVKVLVTH 282
           SNTP  V++V+SPVVKG+ALLLYYKVNV TDNV+   L T+
Sbjct: 241 SNTPTFVTNVNSPVVKGVALLLYYKVNVATDNVIRMSLSTY 281

BLAST of Sed0008584.1 vs. NCBI nr
Match: P98184.1 (RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Bryonia dioica])

HSP 1 Score: 416.0 bits (1068), Expect = 2.6e-112
Identity = 218/282 (77.30%), Postives = 243/282 (86.17%), Query Frame = 0

Query: 1   MKSIGFYFVVALYFGAHFT-DADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVL 60
           M+SIGFY V+ALY GAH T D DINF+L+G TGATYK FI+NLRT LTVG+P V  IPVL
Sbjct: 1   MRSIGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVL 60

Query: 61  KASASGLARFLLVHLANYNGESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQG 120
           + +A+GLARF LV L NYNGES++VALDVVNVYVVAYRAGNTAYFL DAS EAN VLF G
Sbjct: 61  RNAAAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAG 120

Query: 121 FAHVTLPYGGNYDGLETAAGRISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQS 180
             HV LPYGGNYDGLETAAGRISRE I LGFSE+SSAIG+MF +  GTSVP+AFIVIIQ+
Sbjct: 121 INHVRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQT 180

Query: 181 VSEAARFKYIEQRVSENVETKFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRS 240
           VSEAARFKYIEQRVSENV TKF PDPAFLSLQN W  LSEQIQIAQTRGG+FARPVE+R+
Sbjct: 181 VSEAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRT 240

Query: 241 VSNTPIIVSDVSSPVVKGIALLLYYKVNVGTDNVVVKVLVTH 282
           VSNTP  V++V+SPVVKGIALLLY++VNVGTDNV    L T+
Sbjct: 241 VSNTPTFVTNVNSPVVKGIALLLYFRVNVGTDNVFAMSLSTY 282

BLAST of Sed0008584.1 vs. NCBI nr
Match: XP_022158193.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 403.3 bits (1035), Expect = 1.8e-108
Identity = 211/277 (76.17%), Postives = 241/277 (87.00%), Query Frame = 0

Query: 1   MKSIGFYFVVALYFGAHFTDADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLK 60
           M+ + FYF +ALY GA   +AD++F++LG T  TYKQFIKN+R+ LTVGS IV  IPVLK
Sbjct: 1   MRLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLK 60

Query: 61  ASASGLARFLLVHLANYNGESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQGF 120
           A+A+G ARFLLVHL NYN ESI VA+DVVNVYVVAYRAGN AYFLNDASAEA+RVLFQG 
Sbjct: 61  ATATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGI 120

Query: 121 AHVTLPYGGNYDGLETAAGRISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSV 180
            HV L Y GNYDGLETAA +ISREKI LGFSE+SS+IG+MFH+  GTSVP+AFIVIIQSV
Sbjct: 121 NHVKLSYKGNYDGLETAA-KISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSV 180

Query: 181 SEAARFKYIEQRVSENVETKFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSV 240
           SEAARFKYIEQRVSENV+T F PDPAFLSLQNRWSDLSEQIQIAQ RGG+FAR VE+R+V
Sbjct: 181 SEAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTV 240

Query: 241 SNTPIIVSDVSSPVVKGIALLLYYKVNVGTDNVVVKV 278
           SN PI+V+DVSSPVVKGIALLLYYKV+V  D+ ++KV
Sbjct: 241 SNKPILVTDVSSPVVKGIALLLYYKVSVVMDDNIIKV 276

BLAST of Sed0008584.1 vs. NCBI nr
Match: XP_022158192.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 400.2 bits (1027), Expect = 1.5e-107
Identity = 202/281 (71.89%), Postives = 243/281 (86.48%), Query Frame = 0

Query: 1   MKSIGFYFVVALYFGAHFTDADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLK 60
           M+ + FYFV+AL FGA   +AD++F++LG T  TY+QFI+NLR+TLT+ SP+V GIPVL+
Sbjct: 1   MRFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQ 60

Query: 61  ASASGLARFLLVHLANYNGESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQGF 120
           ++A G ARF+LVHL NYN ESI+VA+DVVN+YVVAYRAGN AYFLNDASAEAN VLF+G 
Sbjct: 61  STAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 AHVTLPYGGNYDGLETAAGRISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSV 180
            HV LPY GNYDGLETAAG+ISREKI LGFSE+SS+IG+MFH+  GTS+ +AFIVIIQSV
Sbjct: 121 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 180

Query: 181 SEAARFKYIEQRVSENVETKFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSV 240
            EAARFKYIEQRVSENV+TKF PDPAFLSL+NRWSDLSEQIQIAQ R G+FAR +E+R+V
Sbjct: 181 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 240

Query: 241 SNTPIIVSDVSSPVVKGIALLLYYKVNVGTDNVVVKVLVTH 282
           SN PIIV++VSSP+VKGIALLLYYKV V  DN++    +T+
Sbjct: 241 SNKPIIVTNVSSPIVKGIALLLYYKVRVAIDNIIRMPTMTY 281

BLAST of Sed0008584.1 vs. NCBI nr
Match: XP_022158191.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 341.3 bits (874), Expect = 8.2e-90
Identity = 170/246 (69.11%), Postives = 207/246 (84.15%), Query Frame = 0

Query: 20  DADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLKASASGLARFLLVHLANYNG 79
           +++++F+LLG TG TY+QFI+NLR  LT+ S IV  IPVL A+A   ARF+LVHL NY  
Sbjct: 34  NSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKN 93

Query: 80  ESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQGFAHVTLPYGGNYDGLETAAG 139
           E+I+VA+DVVNVY+VAY+AGN AYFL DAS EA  VLF+G     LPY GNYDGLETAAG
Sbjct: 94  ETITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAG 153

Query: 140 RISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSVSEAARFKYIEQRVSENVET 199
           +ISREKI LGFSE+ SAIG+M+HY  GTSVP+AFIV+IQ++SEAARFKYIE +VS+NVET
Sbjct: 154 KISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVET 213

Query: 200 KFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSVSNTPIIVSDVSSPVVKGIA 259
           KF PDP FLSL+NRWSDLSEQ+QIAQ R G+FARP+EVRSV+N PI+V++V S VV+G+A
Sbjct: 214 KFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMA 273

Query: 260 LLLYYK 266
           LLLY K
Sbjct: 274 LLLYSK 279

BLAST of Sed0008584.1 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 3.4e-115
Identity = 218/282 (77.30%), Postives = 243/282 (86.17%), Query Frame = 0

Query: 1   MKSIGFYFVVALYFGAHFT-DADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVL 60
           M+SIGFY V+ALY GAH T D DINF+L+G TGATYK FI+NLRT LTVG+P V  IPVL
Sbjct: 1   MRSIGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVL 60

Query: 61  KASASGLARFLLVHLANYNGESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQG 120
           + +A+GLARF LV L NYNGES++VALDVVNVYVVAYRAGNTAYFL DAS EAN VLF G
Sbjct: 61  RNAAAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAG 120

Query: 121 FAHVTLPYGGNYDGLETAAGRISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQS 180
             HV LPYGGNYDGLETAAGRISRE I LGFSE+SSAIG+MF +  GTSVP+AFIVIIQ+
Sbjct: 121 INHVRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQT 180

Query: 181 VSEAARFKYIEQRVSENVETKFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRS 240
           VSEAARFKYIEQRVSENV TKF PDPAFLSLQN W  LSEQIQIAQTRGG+FARPVE+R+
Sbjct: 181 VSEAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRT 240

Query: 241 VSNTPIIVSDVSSPVVKGIALLLYYKVNVGTDNVVVKVLVTH 282
           VSNTP  V++V+SPVVKGIALLLY++VNVGTDNV    L T+
Sbjct: 241 VSNTPTFVTNVNSPVVKGIALLLYFRVNVGTDNVFAMSLSTY 282

BLAST of Sed0008584.1 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 211.5 bits (537), Expect = 1.3e-53
Identity = 118/280 (42.14%), Postives = 181/280 (64.64%), Query Frame = 0

Query: 7   YFVVALYFGAHFTDADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLKASASGL 66
           + ++A++ G      D+NF+L   T  TY +FI++ R TL     + + IP+L ++ S  
Sbjct: 9   FLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYD-IPLLYSTISDS 68

Query: 67  ARFLLVHLANYNGESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQGFAHVTLP 126
            RF+L++L +Y  E+ISVA+DV NVYVVAYR  + +YF  ++  EA  +LF+G   +TLP
Sbjct: 69  RRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLP 128

Query: 127 YGGNYDGLETAAGRISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSVSEAARF 186
           Y GNY+ L+TAA +I RE I LG   +SSAI ++F+Y    S P A +V+IQ+ +EAARF
Sbjct: 129 YTGNYENLQTAAHKI-RENIDLGLPALSSAITTLFYY-NAQSAPSALLVLIQTTAEAARF 188

Query: 187 KYIEQRVSENVETKFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSVSNTPII 246
           KYIE+ V++ V T F P+ A +SL+N+WS LS+QI +AQ +GGKF  PV++   +     
Sbjct: 189 KYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQ 248

Query: 247 VSDVSSPVVKG-IALLLYYKVNVGTDNVVVKVLVTHQTTM 286
           V++V S VVKG I LLL  + +   +N +  + +  ++ +
Sbjct: 249 VTNVDSDVVKGNIKLLLNSRASTADENFITTMTLLGESVV 285

BLAST of Sed0008584.1 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.3e-53
Identity = 118/262 (45.04%), Postives = 178/262 (67.94%), Query Frame = 0

Query: 9   VVALYFGAHFTDADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLKASASGLAR 68
           ++A++  A   +AD+ F+L G +  +Y +FI +LR  L   +  V  I +L +SASG +R
Sbjct: 8   ILAIFVAASTVEADVRFSLSGSSSTSYSKFIGDLRKALP-SNGTVYNITLLLSSASGASR 67

Query: 69  FLLVHLANYNGESISVALDVVNVYVVAYRAGNTAYFLNDASAE-ANRVLFQGFAHVTLPY 128
           + L+ L+NY+G++I+VA+DV NVY++ Y   +T+YF N++ A+ A++ +F+G   VTLPY
Sbjct: 68  YTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPY 127

Query: 129 GGNYDGLETAAGRISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSVSEAARFK 188
            GNY+ L+TAAG+I REKI LGF  + SAI ++FHY + T+   AF+VIIQ+ +EA+RFK
Sbjct: 128 SGNYEKLQTAAGKI-REKIPLGFPALDSAITTLFHYDS-TAAAAAFLVIIQTTAEASRFK 187

Query: 189 YIEQRVSENVETKFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSVSNTPIIV 248
           YIE ++ E +    +P  A +SL+N WS LS+QIQ+AQT  G F  PV +       + +
Sbjct: 188 YIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRVEI 247

Query: 249 SDVSSPVV-KGIALLLYYKVNV 269
           ++V+S VV K I LLL YK NV
Sbjct: 248 TNVTSKVVTKNIQLLLNYKQNV 266

BLAST of Sed0008584.1 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.2e-53
Identity = 118/280 (42.14%), Postives = 180/280 (64.29%), Query Frame = 0

Query: 7   YFVVALYFGAHFTDADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLKASASGL 66
           + ++A++ G      D+NF+L   T  TY +FI++ R TL     + + IP+L ++ S  
Sbjct: 9   FLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYD-IPLLYSTISDS 68

Query: 67  ARFLLVHLANYNGESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQGFAHVTLP 126
            RF+L+ L +Y  E+ISVA+DV NVYVVAYR  + +YF  ++  EA  +LF+G   +TLP
Sbjct: 69  RRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLP 128

Query: 127 YGGNYDGLETAAGRISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSVSEAARF 186
           Y GNY+ L+TAA +I RE I LG   +SSAI ++F+Y    S P A +V+IQ+ +EAARF
Sbjct: 129 YTGNYENLQTAAHKI-RENIDLGLPALSSAITTLFYY-NAQSAPSALLVLIQTTAEAARF 188

Query: 187 KYIEQRVSENVETKFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSVSNTPII 246
           KYIE+ V++ V T F P+ A +SL+N+WS LS+QI +AQ +GGKF  PV++   +     
Sbjct: 189 KYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQ 248

Query: 247 VSDVSSPVVKG-IALLLYYKVNVGTDNVVVKVLVTHQTTM 286
           V++V S VVKG I LLL  + +   +N +  + +  ++ +
Sbjct: 249 VTNVDSDVVKGNIKLLLNSRASTADENFITTMTLLGESVV 285

BLAST of Sed0008584.1 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 207.2 bits (526), Expect = 2.4e-52
Identity = 118/257 (45.91%), Postives = 169/257 (65.76%), Query Frame = 0

Query: 9   VVALYFGAHFTDADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLKASASGLAR 68
           ++ L+      + D++F L G T ++Y  FI NLR  L     + + IP+L++S  G  R
Sbjct: 11  ILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYD-IPLLRSSLPGSQR 70

Query: 69  FLLVHLANYNGESISVALDVVNVYVVAYRAGNTAYFLNDASA-EANRVLFQ-GFAHVTLP 128
           + L+HL NY  E+ISVA+DV NVY++ YRAG+T+YF N+ASA EA + +F+     VTLP
Sbjct: 71  YALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMRKVTLP 130

Query: 129 YGGNYDGLETAAGRISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSVSEAARF 188
           Y GNY+ L+TAAG+I RE I LG   + SAI ++F+Y    S   A +V+IQS SEAAR+
Sbjct: 131 YSGNYERLQTAAGKI-RENIPLGLPALDSAITTLFYY-NANSAASALMVLIQSTSEAARY 190

Query: 189 KYIEQRVSENVETKFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSVSNTPII 248
           K+IEQ++ + V+  FLP  A +SL+N WS LS+QIQIA T  G+F  PV + +  N  + 
Sbjct: 191 KFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQRVT 250

Query: 249 VSDVSSPVV-KGIALLL 263
           +++V + VV   IALLL
Sbjct: 251 ITNVDAGVVTSNIALLL 264

BLAST of Sed0008584.1 vs. ExPASy TrEMBL
Match: B1B626 (rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 1.3e-120
Identity = 224/281 (79.72%), Postives = 250/281 (88.97%), Query Frame = 0

Query: 1   MKSIGFYFVVALYFGAHFTDADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLK 60
           MK +GFY V+ALY GA  T+AD+NF+LLG T ATYKQFI+NLR  LTVGSP VE IPVLK
Sbjct: 1   MKLVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLK 60

Query: 61  ASASGLARFLLVHLANYNGESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQGF 120
            +A+GLARFLLVHL NYNGESI+VA+DVVNVYVVAYRAGN AYFLNDASAEAN VLF+G 
Sbjct: 61  GTAAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 AHVTLPYGGNYDGLETAAGRISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSV 180
            HV LPYGGNYDGLETAAGRISREKIYLGFSE+SSAIGSMFHY  GTSVPKAFIVIIQ+V
Sbjct: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTV 180

Query: 181 SEAARFKYIEQRVSENVETKFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSV 240
           SEAARFKYI+QRVSENV+TKFLPDPAFLSL+N W  LSEQIQIAQ RGG+FARP+E+R+V
Sbjct: 181 SEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTV 240

Query: 241 SNTPIIVSDVSSPVVKGIALLLYYKVNVGTDNVVVKVLVTH 282
           SNTP  V++V+SPVVKG+ALLLYYKVNV TDNV+   L T+
Sbjct: 241 SNTPTFVTNVNSPVVKGVALLLYYKVNVATDNVIRMSLSTY 281

BLAST of Sed0008584.1 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 8.5e-109
Identity = 211/277 (76.17%), Postives = 241/277 (87.00%), Query Frame = 0

Query: 1   MKSIGFYFVVALYFGAHFTDADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLK 60
           M+ + FYF +ALY GA   +AD++F++LG T  TYKQFIKN+R+ LTVGS IV  IPVLK
Sbjct: 1   MRLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLK 60

Query: 61  ASASGLARFLLVHLANYNGESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQGF 120
           A+A+G ARFLLVHL NYN ESI VA+DVVNVYVVAYRAGN AYFLNDASAEA+RVLFQG 
Sbjct: 61  ATATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGI 120

Query: 121 AHVTLPYGGNYDGLETAAGRISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSV 180
            HV L Y GNYDGLETAA +ISREKI LGFSE+SS+IG+MFH+  GTSVP+AFIVIIQSV
Sbjct: 121 NHVKLSYKGNYDGLETAA-KISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSV 180

Query: 181 SEAARFKYIEQRVSENVETKFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSV 240
           SEAARFKYIEQRVSENV+T F PDPAFLSLQNRWSDLSEQIQIAQ RGG+FAR VE+R+V
Sbjct: 181 SEAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTV 240

Query: 241 SNTPIIVSDVSSPVVKGIALLLYYKVNVGTDNVVVKV 278
           SN PI+V+DVSSPVVKGIALLLYYKV+V  D+ ++KV
Sbjct: 241 SNKPILVTDVSSPVVKGIALLLYYKVSVVMDDNIIKV 276

BLAST of Sed0008584.1 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 7.2e-108
Identity = 202/281 (71.89%), Postives = 243/281 (86.48%), Query Frame = 0

Query: 1   MKSIGFYFVVALYFGAHFTDADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLK 60
           M+ + FYFV+AL FGA   +AD++F++LG T  TY+QFI+NLR+TLT+ SP+V GIPVL+
Sbjct: 1   MRFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQ 60

Query: 61  ASASGLARFLLVHLANYNGESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQGF 120
           ++A G ARF+LVHL NYN ESI+VA+DVVN+YVVAYRAGN AYFLNDASAEAN VLF+G 
Sbjct: 61  STAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 AHVTLPYGGNYDGLETAAGRISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSV 180
            HV LPY GNYDGLETAAG+ISREKI LGFSE+SS+IG+MFH+  GTS+ +AFIVIIQSV
Sbjct: 121 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 180

Query: 181 SEAARFKYIEQRVSENVETKFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSV 240
            EAARFKYIEQRVSENV+TKF PDPAFLSL+NRWSDLSEQIQIAQ R G+FAR +E+R+V
Sbjct: 181 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 240

Query: 241 SNTPIIVSDVSSPVVKGIALLLYYKVNVGTDNVVVKVLVTH 282
           SN PIIV++VSSP+VKGIALLLYYKV V  DN++    +T+
Sbjct: 241 SNKPIIVTNVSSPIVKGIALLLYYKVRVAIDNIIRMPTMTY 281

BLAST of Sed0008584.1 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 4.0e-90
Identity = 170/246 (69.11%), Postives = 207/246 (84.15%), Query Frame = 0

Query: 20  DADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLKASASGLARFLLVHLANYNG 79
           +++++F+LLG TG TY+QFI+NLR  LT+ S IV  IPVL A+A   ARF+LVHL NY  
Sbjct: 34  NSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKN 93

Query: 80  ESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQGFAHVTLPYGGNYDGLETAAG 139
           E+I+VA+DVVNVY+VAY+AGN AYFL DAS EA  VLF+G     LPY GNYDGLETAAG
Sbjct: 94  ETITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAG 153

Query: 140 RISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSVSEAARFKYIEQRVSENVET 199
           +ISREKI LGFSE+ SAIG+M+HY  GTSVP+AFIV+IQ++SEAARFKYIE +VS+NVET
Sbjct: 154 KISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVET 213

Query: 200 KFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSVSNTPIIVSDVSSPVVKGIA 259
           KF PDP FLSL+NRWSDLSEQ+QIAQ R G+FARP+EVRSV+N PI+V++V S VV+G+A
Sbjct: 214 KFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMA 273

Query: 260 LLLYYK 266
           LLLY K
Sbjct: 274 LLLYSK 279

BLAST of Sed0008584.1 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.6e-83
Identity = 165/249 (66.27%), Postives = 199/249 (79.92%), Query Frame = 0

Query: 20  DADINFNLLGGTGATYKQFIKNLRTTLTVGSPIVEGIPVLKASASGLARFLLVHLANYNG 79
           +A+++F+LLG T  TYKQFI +LR  LTV S  V  IPVL A+A GLARF+LV+L NY G
Sbjct: 33  NANLSFSLLGATAQTYKQFISDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKG 92

Query: 80  ESISVALDVVNVYVVAYRAGNTAYFLNDASAEANRVLFQGFAHVTLPYGGNYDGLETAAG 139
           ESI+VA+D V+VY+VAY+AGN AYFL D S EA+ VLF+G  H TL Y GNYD LE  AG
Sbjct: 93  ESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAG 152

Query: 140 RISREKIYLGFSEMSSAIGSMFHYTTGTSVPKAFIVIIQSVSEAARFKYIEQRVSENVET 199
           +ISRE I LGFSE+SS+IG+MFHY  G SVPK+FIV+IQ +SEA+RFKYIE R  ENVE+
Sbjct: 153 KISRENIDLGFSELSSSIGNMFHYNAGYSVPKSFIVMIQMLSEASRFKYIEARNCENVES 212

Query: 200 KFLPDPAFLSLQNRWSDLSEQIQIAQTRGGKFARPVEVRSVSNTPIIVSDVSSPVVKGIA 259
           KF PDP +LSL+NRWS+LSEQ+Q AQ  GGKF  PV+VRSVSN P++VSDV+SPVVKG+A
Sbjct: 213 KFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMA 272

Query: 260 LLLYYKVNV 269
           LLL  K  V
Sbjct: 273 LLLCNKPGV 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
BAG12408.12.6e-12079.72karasurin-H precursor [Trichosanthes kirilowii var. japonica][more]
P98184.12.6e-11277.30RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltNa... [more]
XP_022158193.11.8e-10876.17ribosome-inactivating protein bryodin II-like [Momordica charantia][more]
XP_022158192.11.5e-10771.89ribosome-inactivating protein bryodin II-like [Momordica charantia][more]
XP_022158191.18.2e-9069.11ribosome-inactivating protein bryodin II-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
P981843.4e-11577.30Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1[more]
P248171.3e-5342.14Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN... [more]
Q004651.3e-5345.04Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1[more]
P293392.2e-5342.14Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV... [more]
P099892.4e-5245.91Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=... [more]
Match NameE-valueIdentityDescription
B1B6261.3e-12079.72rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1[more]
A0A6J1DVE78.5e-10976.17rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1[more]
A0A6J1DWK47.2e-10871.89rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1[more]
A0A6J1E0934.0e-9069.11rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1[more]
A0A6J1IUB21.6e-8366.27rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017989Ribosome-inactivating protein type 1/2PRINTSPR00396SHIGARICINcoord: 81..96
score: 52.8
coord: 173..193
score: 61.9
coord: 205..222
score: 43.27
coord: 143..157
score: 42.98
coord: 33..46
score: 40.6
IPR016138Ribosome-inactivating protein, subdomain 1GENE3D3.40.420.10Ricin (A subunit), domain 1coord: 22..185
e-value: 3.3E-40
score: 139.5
IPR016139Ribosome-inactivating protein, subdomain 2GENE3D4.10.470.10Ricin (A Subunit), domain 2coord: 186..268
e-value: 3.2E-26
score: 93.0
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 34..222
e-value: 2.7E-32
score: 112.0
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 23..266
NoneNo IPR availablePANTHERPTHR33453:SF14RRNA N-GLYCOSIDASEcoord: 23..266
IPR017988Ribosome-inactivating protein conserved sitePROSITEPS00275SHIGA_RICINcoord: 177..193
IPR036041Ribosome-inactivating protein superfamilySUPERFAMILY56371Ribosome inactivating proteins (RIP)coord: 22..268

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Sed0008584Sed0008584gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0008584.1-three_prime_utrSed0008584.1-three_prime_utr-LG09:4853857..4854026three_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0008584.1-exonSed0008584.1-exon-LG09:4853857..4854935exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0008584.1-cdsSed0008584.1-cds-LG09:4854027..4854887CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0008584.1-five_prime_utrSed0008584.1-five_prime_utr-LG09:4854888..4854935five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Sed0008584.1Sed0008584.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017148 negative regulation of translation
biological_process GO:0006952 defense response
biological_process GO:0035821 modulation of process of other organism
molecular_function GO:0030598 rRNA N-glycosylase activity
molecular_function GO:0090729 toxin activity