Sed0007686.1 (mRNA) Chayote v1

Overview


Name	Sed0007686.1
Type	mRNA
Organism	Sechium edule (Chayote v1)
Description	SpoU_methylase domain-containing protein
Location	LG12: 5491209 .. 5494348 (-)
Sequence length	657
RNA-Seq Expression	Sed0007686.1
Synteny	Sed0007686.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGTTCGAAAGCTACGTCGTGGTGCACAACATCGCCAAACGCCACAACGTCGGCACATTGGCTCGAAGCGCCACCGCCTTCGGCGTTTCCGAGCTCATCCTCGTCGGCCGTCGCGATTTCAACGCCTTCGGCAGCCATGGTTCCACTTCTCACGTCCGCTTCCGCCATTTCCATTCCCTTCTCGACGCCCAGAATTTCCTCAAGGTTCGGCCCTTTTTCTTAATTCTGTAATGATGGGTCTAAATTTTGAATTGGTTTCTTCTGTATCTGTAGGAGAAAGATTGCGATATATGTGGGGTGGAGATCACTGACAACGCCGTGGCTGTTAATCAGCATCCTTTCAAGAGGAGTACTGCTTTTCTTCTTGGCAATGAGGTTGGTTCTTCGTTTGCATCTTGTTGGCTGGTTATTAATCGTGACCCCTACATGCTTGTATTGGAATTCATATTTTGGTTGCATTTATTAAAAACCCAGTAGCAGGAGTAACTACTTAAGGTCATGTTTGGATGGGGAAGAATAAAGTGGTTTGAATGGTGGTTGCACAAAAATAATGTTTGGATAAGATTGTTTGAAGGGGTGTCGGAGAAAATTGTGTTTGAATGAGTGATTGCAAATAAATCTAGTGAGGTAGGAAAAATGTTGTTTTGAAGAGGGGTTGTCCCATTGTAGGAAAGCTTTGTTTGGAAAAAGGTTGTTTGGAAGAGGGGTTTGAGTGAGGAGTTGGAATGTGGGGTTGGAAGAAGTTTTTTCTACTCTAAACAACCATCGGAAAATCTTACCCCAGATTCCTTCCTCCATATCCATGCATGCTCTGGTAGTTCTTTAATCTGCATACAAAACCTTTGTTGTTTGATCTGTTCTTGATCTATTTCTAGGAATTTTCTCTTCGCTGCAATTTATGATGTTCGAAATGATAGGATTGTTTTTTATATACTGCAGTAAGTTAGCAAGTTCCATTAGACATATAGCATGAAAATATTTTTGTCCCTCTTTGATTCCTTCATTTTTCTCATTGAAAACTCAGTTTCTTCTTAAGGTGGACTGAACATACTTTATTAGTGGGGATGCCATGAGTATAATGCAAGGAGTGAGCTTCGGTTCCATGATTAGAATAGATAACCGATGAATGCAGTCCCCAGGGGGTGGTACAATGGTTGAAGACTTGGGCTTTGAAGGTGTGCTCCCTTCAAGGTCTCAAGTTCGAGACTCACCTATGACATTAATTTGTAGACACCTCTTGTGTCTCCATTCCTTCTATGTCTCTTGGTGCTTGGCGAAGTTCCAATTTCTCGGTTTCAAAAAAAATAAAAAATAATAACCAATGACTGCTTAATGTTCCTCTGGTAATAATATTTTCTGTAAGAGATTGTTTTTTTTTTCCACTTTTGATGTATTGTGATGAGATTATGACTTAATATGTCATTATTGCTTGCCTTATTGTTGGATCCATATCTCTATGATGTACTCGACAATGAGACTTAGTAGATTATCATCTGGACTATATTCTGCAGTCAGATAGCAAGTTCCTTTCGAACTTCTTTGGCATATTCCTTTCCAAATCCTTTGATTAAACCTCTATTGTAAGCTGTCATATTTTTCCATTATTTTCATTATATATATATATATATGTATCATTTGGAAATAATTCTGTTACACTATTTTCAGCATTGAGCCACCCTTTTGATGGTTATGTGCTTATCGTTTCTAGGGAACAGGGCTTTCTGCTAAAGAGTGTGCAATCTGTGACTTCTTCATTTACATCCCACAGTATGGTGTCGGCACTGCTTCTTTGAATGTAACTGTAGCTGCTTCCATTGTCCTACATCATTTTGGTGGTAATTGATTCAGAGCTATTGCATTTTGACTCCCCCGTTTGAGTTTATTTTGCATACATGAATATATATCACCTATAAGGTTTCCTATATATATATATGAATACCTCTCTCTAATATGCTCATAGTTTTTGTCTTTTGGTTTTTGACCAAGCTAACCCATGAGTAGTGTAAAATTACACAACCAATGAAAGCACTGTTGGAAGAAATGCATCTAAATCTTAAAAACTTTAAAGTACGCAAGGAGGAGCAGATATCTGATGTTTACTCATTTTCTGACTCATGAATCAGTTCACAACGGAAAATGATCCCTTCAAGCTGAAACTAAGGAATAACTATGGCTCAGTTGAATCGAATGATGGTGCCAGTTTGTAATAGGCTAATAGCTCTCATGCTTGATATTGATGACCATCTAGGTTTACTTAAAAAAAAAATGATCATGTAGGTGGATTGATAAATCATCCTCTCGTTGTCAAGTATACATGGGCTTCTATATATATATGTTAACAAGAGCAGTTGCTCAACGGCATCTTAGTGTACACTTAATGACCAAAAGGTCTTAGGTTCGAGTCCCCCACCTCCTAATTGTTGTAATCTAAAAAAAAAACTATACATGGGCTGCTGTATTTGATGTTTTCTTTTTACTTGCAAGTTCCGCATATTGTTCACTTAACTCTCATGACATCGAAACAACTTATTTGGGAGCTGATCTATTTTTTATTTTTAAGAACAGTGATACAGTAGCTTGTAAAGGCCGGGCATTTATGAGCCCGTATTTTTGAAGGAAGAACGATTTAGTTTGAATTGAAAAAACATGTCACCTATGAGAACAAACTAAAAAAAATCGTAGTTGTTTTTTCAAATATGTTTTACTGGATTTTATTTATTCATTTTTTTAATTGTGTTTTCTAAAACAGCAGATTTAGCCATATCTCCTATAGACCTGTACCATGTGAGGATTTTTTTTTCTTTTTGAAAACCCGAGAATTCTTTCTAGTATGCATATATGTTGAGGTTTGAAAAAATGGAAACGGTACCTGTCTCCATTTCTAGATGTTATTAACTTCTAGATCATATTTTTCAGTTTGGGCAGGATTTCCCGAAAGATGTCGCGATGGTAACAAGTTTATAGTGGCTGAAAGACCAGTAAAACAGGTCAAGCGGAATTATTGCACCGAAACAGAAGAATCTATCACTGAAGAACGAAAATCGAGGAGGGAGAATGCTTCTAAGGACTTCTTTGATGAGGGAATGATTGATGATTCAACATCTAACCTTCTTGATACATTGTATATCGAAGAATGA

mRNA sequence

ATGGGGTTCGAAAGCTACGTCGTGGTGCACAACATCGCCAAACGCCACAACGTCGGCACATTGGCTCGAAGCGCCACCGCCTTCGGCGTTTCCGAGCTCATCCTCGTCGGCCGTCGCGATTTCAACGCCTTCGGCAGCCATGGTTCCACTTCTCACGTCCGCTTCCGCCATTTCCATTCCCTTCTCGACGCCCAGAATTTCCTCAAGGAGAAAGATTGCGATATATGTGGGGTGGAGATCACTGACAACGCCGTGGCTGTTAATCAGCATCCTTTCAAGAGGAGTACTGCTTTTCTTCTTGGCAATGAGGGAACAGGGCTTTCTGCTAAAGAGTGTGCAATCTGTGACTTCTTCATTTACATCCCACAGTATGGTGTCGGCACTGCTTCTTTGAATGTAACTGTAGCTGCTTCCATTGTCCTACATCATTTTGGTGTTTGGGCAGGATTTCCCGAAAGATGTCGCGATGGTAACAAGTTTATAGTGGCTGAAAGACCAGTAAAACAGGTCAAGCGGAATTATTGCACCGAAACAGAAGAATCTATCACTGAAGAACGAAAATCGAGGAGGGAGAATGCTTCTAAGGACTTCTTTGATGAGGGAATGATTGATGATTCAACATCTAACCTTCTTGATACATTGTATATCGAAGAATGA

Coding sequence (CDS)

ATGGGGTTCGAAAGCTACGTCGTGGTGCACAACATCGCCAAACGCCACAACGTCGGCACATTGGCTCGAAGCGCCACCGCCTTCGGCGTTTCCGAGCTCATCCTCGTCGGCCGTCGCGATTTCAACGCCTTCGGCAGCCATGGTTCCACTTCTCACGTCCGCTTCCGCCATTTCCATTCCCTTCTCGACGCCCAGAATTTCCTCAAGGAGAAAGATTGCGATATATGTGGGGTGGAGATCACTGACAACGCCGTGGCTGTTAATCAGCATCCTTTCAAGAGGAGTACTGCTTTTCTTCTTGGCAATGAGGGAACAGGGCTTTCTGCTAAAGAGTGTGCAATCTGTGACTTCTTCATTTACATCCCACAGTATGGTGTCGGCACTGCTTCTTTGAATGTAACTGTAGCTGCTTCCATTGTCCTACATCATTTTGGTGTTTGGGCAGGATTTCCCGAAAGATGTCGCGATGGTAACAAGTTTATAGTGGCTGAAAGACCAGTAAAACAGGTCAAGCGGAATTATTGCACCGAAACAGAAGAATCTATCACTGAAGAACGAAAATCGAGGAGGGAGAATGCTTCTAAGGACTTCTTTGATGAGGGAATGATTGATGATTCAACATCTAACCTTCTTGATACATTGTATATCGAAGAATGA

Protein sequence

MGFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHVRFRHFHSLLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIYIPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQVKRNYCTETEESITEERKSRRENASKDFFDEGMIDDSTSNLLDTLYIEE

Homology

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BLAST of Sed0007686.1 vs. NCBI nr
Match: XP_008438758.1 (PREDICTED: putative TrmH family tRNA/rRNA methyltransferase [Cucumis melo])

HSP 1 Score: 427.6 bits (1098), Expect = 6.6e-116
Identity = 207/218 (94.95%), Postives = 213/218 (97.71%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MG ESYVVVHNIAKRHNVGT+ARSATAFGVSELILVGRRDFNAFGSHGSTSH+RFRHFHS
Sbjct: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHIRFRHFHS 60

Query: 61  LLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDAQ FLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPV+QVKRNYC ETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVRQVKRNYCAETEE 180

Query: 181 SITEERKSRRENASKDFFDEGMIDDSTSNLLDTLYIEE 219
           SI EERKSRRENASK FFDEGMIDDSTSNLLDTL+I++
Sbjct: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 218

[Close]

BLAST of Sed0007686.1 vs. NCBI nr
Match: KAA0049420.1 (putative TrmH family tRNA/rRNA methyltransferase [Cucumis melo var. makuwa] >TYK16098.1 putative TrmH family tRNA/rRNA methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 426.8 bits (1096), Expect = 1.1e-115
Identity = 207/218 (94.95%), Postives = 213/218 (97.71%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MG ESYVVVHNIAKRHNVGT+ARSATAFGVSELILVGRRDFNAFGSHGSTSH+RFRHFHS
Sbjct: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60

Query: 61  LLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDAQ FLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPV+QVKRNYC ETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVRQVKRNYCAETEE 180

Query: 181 SITEERKSRRENASKDFFDEGMIDDSTSNLLDTLYIEE 219
           SI EERKSRRENASK FFDEGMIDDSTSNLLDTL+I++
Sbjct: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 218

[Close]

BLAST of Sed0007686.1 vs. NCBI nr
Match: KAG7028939.1 (tRNA (guanosine(18)-2'-O)-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 424.5 bits (1090), Expect = 5.6e-115
Identity = 207/218 (94.95%), Postives = 212/218 (97.25%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSE+ILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 60

Query: 61  LLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDA+NFL+EKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 61  LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQ KRNYC ETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 180

Query: 181 SITEERKSRRENASKDFFDEGMIDDSTSNLLDTLYIEE 219
           SI EERKSRR+NASK FFDEG IDDSTSNLLDTL+I E
Sbjct: 181 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 218

[Close]

BLAST of Sed0007686.1 vs. NCBI nr
Match: XP_022953849.1 (uncharacterized protein LOC111456233 isoform X1 [Cucurbita moschata])

HSP 1 Score: 424.5 bits (1090), Expect = 5.6e-115
Identity = 207/218 (94.95%), Postives = 212/218 (97.25%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSE+ILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 93  MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 152

Query: 61  LLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDA+NFL+EKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 153 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 212

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQ KRNYC ETEE
Sbjct: 213 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 272

Query: 181 SITEERKSRRENASKDFFDEGMIDDSTSNLLDTLYIEE 219
           SI EERKSRR+NASK FFDEG IDDSTSNLLDTL+I E
Sbjct: 273 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 310

[Close]

BLAST of Sed0007686.1 vs. NCBI nr
Match: XP_022953920.1 (uncharacterized protein LOC111456233 isoform X2 [Cucurbita moschata])

HSP 1 Score: 424.5 bits (1090), Expect = 5.6e-115
Identity = 207/218 (94.95%), Postives = 212/218 (97.25%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSE+ILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 86  MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 145

Query: 61  LLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDA+NFL+EKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 146 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 205

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQ KRNYC ETEE
Sbjct: 206 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 265

Query: 181 SITEERKSRRENASKDFFDEGMIDDSTSNLLDTLYIEE 219
           SI EERKSRR+NASK FFDEG IDDSTSNLLDTL+I E
Sbjct: 266 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 303

[Close]

BLAST of Sed0007686.1 vs. ExPASy Swiss-Prot
Match: Q4L3J1 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus haemolyticus (strain JCSC1435) OX=279808 GN=SH2477 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 6.0e-11
Identity = 47/146 (32.19%), Postives = 71/146 (48.63%), Query Frame = 0


Query: 2   GFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFN-----AFGSHGSTSHVRFR 61
           G  + V++  +   HN+G++ R+A A GV  +I+  RR        A  S G+  HV   
Sbjct: 93  GLSTVVILDGLEDPHNLGSILRTADASGVDGVIIPKRRSVALTQTVAKASTGAIQHVPVM 152

Query: 62  HFHSLLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L    + LKE    + G E  DNA            A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKEHGYWVAGAE-ADNATDYRDMAADMPLAIVIGSEGQGMSRLVKDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of Sed0007686.1 vs. ExPASy Swiss-Prot
Match: Q99W72 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) OX=158878 GN=SAV0531 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 45/146 (30.82%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 2   GFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFN-----AFGSHGSTSHVRFR 61
           G  + +++  +   HN+G++ R+A A GV  +I+  RR        A  S G+  HV   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L    + LK+    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of Sed0007686.1 vs. ExPASy Swiss-Prot
Match: Q7A794 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain N315) OX=158879 GN=SA0490 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 45/146 (30.82%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 2   GFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFN-----AFGSHGSTSHVRFR 61
           G  + +++  +   HN+G++ R+A A GV  +I+  RR        A  S G+  HV   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L    + LK+    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of Sed0007686.1 vs. ExPASy Swiss-Prot
Match: Q6GJD7 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=SAR0535 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 45/146 (30.82%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 2   GFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFN-----AFGSHGSTSHVRFR 61
           G  + +++  +   HN+G++ R+A A GV  +I+  RR        A  S G+  HV   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L    + LK+    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of Sed0007686.1 vs. ExPASy Swiss-Prot
Match: Q6GBV6 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain MSSA476) OX=282459 GN=SAS0489 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 45/146 (30.82%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 2   GFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFN-----AFGSHGSTSHVRFR 61
           G  + +++  +   HN+G++ R+A A GV  +I+  RR        A  S G+  HV   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L    + LK+    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of Sed0007686.1 vs. ExPASy TrEMBL
Match: A0A1S3AXA1 (putative TrmH family tRNA/rRNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483771 PE=4 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 3.2e-116
Identity = 207/218 (94.95%), Postives = 213/218 (97.71%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MG ESYVVVHNIAKRHNVGT+ARSATAFGVSELILVGRRDFNAFGSHGSTSH+RFRHFHS
Sbjct: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHIRFRHFHS 60

Query: 61  LLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDAQ FLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPV+QVKRNYC ETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVRQVKRNYCAETEE 180

Query: 181 SITEERKSRRENASKDFFDEGMIDDSTSNLLDTLYIEE 219
           SI EERKSRRENASK FFDEGMIDDSTSNLLDTL+I++
Sbjct: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 218

[Close]

BLAST of Sed0007686.1 vs. ExPASy TrEMBL
Match: A0A5A7U267 (Putative TrmH family tRNA/rRNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00200 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 5.5e-116
Identity = 207/218 (94.95%), Postives = 213/218 (97.71%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MG ESYVVVHNIAKRHNVGT+ARSATAFGVSELILVGRRDFNAFGSHGSTSH+RFRHFHS
Sbjct: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60

Query: 61  LLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDAQ FLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPV+QVKRNYC ETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVRQVKRNYCAETEE 180

Query: 181 SITEERKSRRENASKDFFDEGMIDDSTSNLLDTLYIEE 219
           SI EERKSRRENASK FFDEGMIDDSTSNLLDTL+I++
Sbjct: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 218

[Close]

BLAST of Sed0007686.1 vs. ExPASy TrEMBL
Match: A0A6J1IDR0 (uncharacterized protein LOC111472230 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472230 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 2.7e-115
Identity = 207/218 (94.95%), Postives = 212/218 (97.25%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSE+ILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 108 MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 167

Query: 61  LLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDA+NFL+EKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 168 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 227

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQ KRNYC ETEE
Sbjct: 228 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 287

Query: 181 SITEERKSRRENASKDFFDEGMIDDSTSNLLDTLYIEE 219
           SI EERKSRR+NASK FFDEG IDDSTSNLLDTL+I E
Sbjct: 288 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 325

[Close]

BLAST of Sed0007686.1 vs. ExPASy TrEMBL
Match: A0A6J1GR06 (uncharacterized protein LOC111456233 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456233 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 2.7e-115
Identity = 207/218 (94.95%), Postives = 212/218 (97.25%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSE+ILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 93  MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 152

Query: 61  LLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDA+NFL+EKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 153 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 212

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQ KRNYC ETEE
Sbjct: 213 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 272

Query: 181 SITEERKSRRENASKDFFDEGMIDDSTSNLLDTLYIEE 219
           SI EERKSRR+NASK FFDEG IDDSTSNLLDTL+I E
Sbjct: 273 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 310

[Close]

BLAST of Sed0007686.1 vs. ExPASy TrEMBL
Match: A0A6J1GR02 (uncharacterized protein LOC111456233 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456233 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 2.7e-115
Identity = 207/218 (94.95%), Postives = 212/218 (97.25%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSE+ILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 86  MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 145

Query: 61  LLDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDA+NFL+EKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 146 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 205

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQ KRNYC ETEE
Sbjct: 206 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 265

Query: 181 SITEERKSRRENASKDFFDEGMIDDSTSNLLDTLYIEE 219
           SI EERKSRR+NASK FFDEG IDDSTSNLLDTL+I E
Sbjct: 266 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 303

[Close]

BLAST of Sed0007686.1 vs. TAIR 10
Match: AT4G15520.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 347.8 bits (891), Expect = 6.2e-96
Identity = 170/220 (77.27%), Postives = 195/220 (88.64%), Query Frame = 0


Query: 2   GFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHVRFRHFHSL 61
           GFESYVVVHNIAKRHNVGTLARSATAFGV+ELILVGRRDFNAFGSHGS SH+RFRHFHSL
Sbjct: 3   GFESYVVVHNIAKRHNVGTLARSATAFGVTELILVGRRDFNAFGSHGSASHIRFRHFHSL 62

Query: 62  LDAQNFLK-EKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 121
           ++A+N+LK EKDCDICGVEI D A AVN+HPFKR+TAFLLGNEG+GLSAKE  ICDFF+Y
Sbjct: 63  IEARNYLKEEKDCDICGVEIADGASAVNEHPFKRNTAFLLGNEGSGLSAKEYEICDFFVY 122

Query: 122 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQVKRNYCTETEE 181
           IPQYG GTASLNVTVAASIVLHHFGVWAGF ER RDG+KFIVA+RPV+Q +RN+C  TEE
Sbjct: 123 IPQYGCGTASLNVTVAASIVLHHFGVWAGFSERVRDGSKFIVADRPVRQGRRNFCAGTEE 182

Query: 182 SITEERKSRRENASKDFFDEGMIDD--STSNLLDTLYIEE 219
           S+ EERK R+E+A   FFD+   ++  S+S+LLD L++ E
Sbjct: 183 SVIEERKLRKESAENGFFDDNGNENGSSSSDLLDGLFLNE 222

[Close]

BLAST of Sed0007686.1 vs. TAIR 10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 55.5 bits (132), Expect = 6.4e-08
Identity = 38/142 (26.76%), Postives = 66/142 (46.48%), Query Frame = 0


Query: 7   VVVHNIAKRHNVGTLARSATAFGVSELILVG-----RRDFNAFGSHGSTSHVRFRHFHSL 66
           +VV  ++   N+    RSA A G+  + +V      R + N   S G+   +    + + 
Sbjct: 157 LVVEGLSDFGNISAAFRSADALGIQSVHVVSCDSSKRYNGNRHVSMGAEKWLDIEFWDTP 216

Query: 67  LDAQNFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIYI 126
            +    LK +   I    +  + V++    +   TA ++GNEG G+S +   + D    I
Sbjct: 217 KECFKVLKSRGYRIATTHLGMDTVSIYDMDWSCPTAIVVGNEGRGISDEALELSDLRCSI 276

Query: 127 PQYGVGTASLNVTVAASIVLHH 144
           P  G+   S NV+VAA I++HH
Sbjct: 277 PMNGM-VDSFNVSVAAGILMHH 297

The following BLAST results are available for this feature:

BLAST of Sed0007686.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008438758.1	6.6e-116	94.95	PREDICTED: putative TrmH family tRNA/rRNA methyltransferase [Cucumis melo]	[more]
KAA0049420.1	1.1e-115	94.95	putative TrmH family tRNA/rRNA methyltransferase [Cucumis melo var. makuwa] >TYK...	[more]
KAG7028939.1	5.6e-115	94.95	tRNA (guanosine(18)-2'-O)-methyltransferase [Cucurbita argyrosperma subsp. argyr...	[more]
XP_022953849.1	5.6e-115	94.95	uncharacterized protein LOC111456233 isoform X1 [Cucurbita moschata]	[more]
XP_022953920.1	5.6e-115	94.95	uncharacterized protein LOC111456233 isoform X2 [Cucurbita moschata]	[more]

BLAST of Sed0007686.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4L3J1	6.0e-11	32.19	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus haemolyticus ...	[more]
Q99W72	1.8e-10	30.82	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
Q7A794	1.8e-10	30.82	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
Q6GJD7	1.8e-10	30.82	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
Q6GBV6	1.8e-10	30.82	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]

BLAST of Sed0007686.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3AXA1	3.2e-116	94.95	putative TrmH family tRNA/rRNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A5A7U267	5.5e-116	94.95	Putative TrmH family tRNA/rRNA methyltransferase OS=Cucumis melo var. makuwa OX=...	[more]
A0A6J1IDR0	2.7e-115	94.95	uncharacterized protein LOC111472230 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1GR06	2.7e-115	94.95	uncharacterized protein LOC111456233 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GR02	2.7e-115	94.95	uncharacterized protein LOC111456233 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of Sed0007686.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15520.1	6.2e-96	77.27	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT5G15390.1	6.4e-08	26.76	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 6..142 e-value: 1.5E-28 score: 99.8
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 3..153 e-value: 3.8E-39 score: 136.1
None	No IPR available	PANTHER	PTHR43191	RRNA METHYLTRANSFERASE 3,	coord: 2..187
None	No IPR available	PANTHER	PTHR43191:SF10	BNAA01G18460D PROTEIN	coord: 2..187
None	No IPR available	CDD	cd18096	SpoU-like	coord: 5..144 e-value: 9.9585E-77 score: 225.557
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 5..145

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sed0007686	Sed0007686	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0007686.1-cds	Sed0007686.1-cds-LG12:5491209..5491429	CDS
Sed0007686.1-cds	Sed0007686.1-cds-LG12:5492511..5492637	CDS
Sed0007686.1-cds	Sed0007686.1-cds-LG12:5493971..5494072	CDS
Sed0007686.1-cds	Sed0007686.1-cds-LG12:5494142..5494348	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0007686.1-exon	Sed0007686.1-exon-LG12:5491209..5491429	exon
Sed0007686.1-exon	Sed0007686.1-exon-LG12:5492511..5492637	exon
Sed0007686.1-exon	Sed0007686.1-exon-LG12:5493971..5494072	exon
Sed0007686.1-exon	Sed0007686.1-exon-LG12:5494142..5494348	exon

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sed0007686.1	Sed0007686.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006396	RNA processing
biological_process	GO:0016310	phosphorylation
biological_process	GO:0015979	photosynthesis
biological_process	GO:0006090	pyruvate metabolic process
biological_process	GO:0001510	RNA methylation
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0050242	pyruvate, phosphate dikinase activity