Sed0007632.5 (mRNA) Chayote v1

Overview


Name	Sed0007632.5
Type	mRNA
Organism	Sechium edule (Chayote v1)
Description	meiotic recombination protein SPO11-2
Location	LG09: 8354747 .. 8359632 (-)
Sequence length	1391
RNA-Seq Expression	Sed0007632.5
Synteny	Sed0007632.5

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGAAGAACATCTTGAGCCAAACAAGGTGTTGGGCTTCATCACCTCACCTCACCTCATCTCCTCTTAAAACAAACGCCCCCTTAACATTGCAGATAACTTCCTCCAAGTAATTGAAGAATTTGAATATCTAACTTCCTCTTTTTCTCTGTTTTTTGAGTTTCTTTCTCTACAAATAGATCGCAACAGAACGTTTCAAAAATTCAAATACGCTATGGAGCAGACAGTGAGTTCATTCGAATCCTCCATGAGATTTTTCTCCGACCAAGAACTCTGCTACGCCGATCTCCTCACTCCTCAACAGGTTGGATCTTCGCTTCATTCGTAATCTCCGATCACTGTTCTTTCATCTCAAGCTCCGATTCCGAACAGGTGATTGCAAGAATCCAAGTCGCCGTTCTCAACTTCCTCACGATTCTCACTTCCTCCTCTCCTGCCATCTCGAATCTCCCTCTGGTACGATTTCTATCCAAGAAATGGCACGAAATGAGTTTTATGTACTTCGATTTCTTGCTCTTTCCGTTTTTGAAACCCTAGGTTGTTCTCTGTTCCTGATTGAACAGATCGATCGACGATCGAGCAATAGCCGAGTGAATCGAGGCATTTTGACGGAAGAATCGTGTATTTTCCTTTCGAATTCGTTTTGTACGCGGTCTTTGATGCGATCTAATGCTGCGAAGGCTTTTGTTAGAGGTAATGTAATCATCGAACCTTAATTCTCGGCGGTCAGGTTCTTCGAATGTTGAATTTGTTCGGAGTTGTTTGGATGGTGTTTTGTAGGAGGAAATTAGTTTGATGAGTTATTTTGAGTAGTTAGAATTTGAGAGTTGCAACCAAAAAGTTGTTTGGATGTTATTTTTACTTAGAGGAGTTAGAGACACAATAACCAGTTATTTGAATAGTTAAAAAAGAATTATTAAAAAAGACCGGTTAAGAATGAGTTATTTTGAATATTTAAAAATGAATGATTAAAAATGAGTGGTTAGAAATGAGGAGTTATTTTGAATAGTTAAAAATGAGTGATTAGAAATGAGGAGTTATTTTGAGTAGTTAGAAATTAGTTGTTAGAAATGAAAAGTTATTTTGAGTAGTTAGAAATTAGTTGTTAGAAATGAAAAGTTATTTTGAGTAATTAGAAATTAGTTGTTAAAAATGAGGACTTATTTTGAGTAGTTAGAAATGAATGATTAGAAATTAGTTCTTACAAAGGAAGAGTTATTTTAAGTAATTAGAAATGATGATTAGAAATTAGTTGTTAAAAATGAGGACTTATTTTGACTAATTAGAAATGAATGATTAGAAATGAAGAATTAGTTTTAGTAGTAAAAATAAGGAGTATTCTTGAGGGGTGGTGTAGTCCTTGAAGTCTTGGATTTTGAAAGTACACTCCCCTCAAGGTCCTAAGTTCGAGACTCACATGTGACAGTAATTTGTAGACACCTCCCGCGTCGTTATTCCTTTGATGTCTCCTCATGCCCTAGGCCGAAAAATAAGGAGTTGTTTTTGAAGTGGGGTTAGTAAATACTCATTTTAAATCCTCCATTAATTCAACAAACAAACACCCTTCTATTTTGGTCAATTGTGCTGTAGTGTGGAAGCTGATGGAGATTTGCTCTCAGATTCTGGGTCAGGAAAAGCGAGTCACACAGAGGGAGCTTTTCTACAAGTTGATATGTGATTCACCTGCCTATTTCCCAACTCAATTGCATGTCAATAGAACAATTCAAGGTGATTTTGTGTTCTATGAACAAACACTGGCTGGTTTAAGGCCTAAAATTACATTGAACATAGTGATGAAATTTGGGTAGTTTTGTTTTTGTAGATGTTGTAGCTTTACTTCGATGCAGCCGTTACAGCCTTGGAATTATGGCATCTAGTAGGGGACTTGTAGCTGGGCGTCTTTTGATTCAGGCACATTGCTATGTCTTTTATTTTTGGATCTTATATTGCAATATAAAAGTATTCAAAATTACATGATAACCAAGTCATTTGAAGAACTTGAATTGTCCTTAATCTTGAGATCACTTAAATACTTTTTGTTTTATATCTAGCTGAGCATTCGAATCAGCTAACATGCACCTTGTTTATTATCACTGAACAAATGGTCTGATCCTTCAACATTTGACTGTCAAATAAACTTGTATAAAATTAACTGAGGTAAATGGCCAACAAAGGAGTAGTACTCCAATAACATTATGTATAACATGGTAACCAGTTGATTACCGGTCGAGTCCCAAATGTGGTTGAATTCAAAATTATTAGGTAGGTGGTTACTAAAGATTGAACTCATAATTTCTTAAAAAGTACAAAGTCCTATTTGATCACTAGGCCGATGGTTAAAGTCTTAGAATACTTATTACTCAGTGAATAATTGCATACTTTCTTCTATATACAGCACCTCTATTTTATAAATTATAACTAAATGTCGAGTAAATTCTTATATCCTATCATATGCATGAGATGTAAATTGGCTAGTATTGAGTCCCTCTTTTTCTATTTTCACATCAATATTACTTTGAAACCATTGCCAATTGCTAATTTATGTATAGTTTTATTTCTTGGTATCTGGCCAGCACACACTTATAAGGTTTCTCATATTGAACCAAATTCTTGAATGTTGAATCCTTTATAAACAATTTCAATTGCTGTAGGAGCCAGAGCAAGAAACTGTGGACTGCACTGCCTGTGGTTCCTCTGGATATGCCATTTCCGGCGACTTGGACCTGCTGCAGAAGTTGATTCTCAAGACCGACGCCCGGTACATAATTGTGATCGAAAAGGTGATTTAAGTTTCTGAAGATATGATTTGCTAGTTAATTATACTTTTGTGAAGACAAAAAATGTGAAAAATGTAGTCAACTACCAAACATTGTTGGTTATACAGCACGCAATATTCCAGCGGCTGGCGGAGGATTGTGTTTTCAACCACATTCCCAGTATTCTTATAACAGCCAAAGGATATCCAGACATAGCAACAAGGTAAGTTTGTTCAACTAGTTTCGAAGATCGCAGTTCCTCGATGCGTTCTTGAAGCCGTAACTAAAACTCTGTGATCTGCCTTTTCAGGTATTTTCTTCACAAAATCAGCAAAATTTTCCCCCATCTGCCGATTTTCGGCCTTGTTGATTGGTATGATCCATTTTAACATCAACTCAGCAACAAGGTTGTAGAACTGTGTTCTCACTTTTAGCATTTGGGGTTCTAAAGGAACCCTGCTGGATTGGCTATATTGTGCACCTTCAAGTATGGAAGTATTGGGATGGGCTTGGAGGCATATAGATATGGTATGTCTCAGTTCCTTGATTGTTCTATCCATTTCTGATTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTAAGATGTTCAAACATTTGGATTTTGGTTTTTTTTCTTTTTTGAACTAAAGGATGTTTTATAGCCAAATTACAAAAAGGAAAACAAGTTTTTTATTTTTTGTTTTAAAAAGTAAAAACCATATATGTTTGATAACTCATTTTAGTTTTATGTTTTTAAAAAATGAAAAACCAAAAAACATGTTCGACAACTGTTTTTTGTTTTCAAATATCACATTTTATTTGGATTTTCTTAGTTTTTTACTATTTTTTCTTGTTCTTTTCCTACTTTTTTTTAAACAAAAACTTGTTTTTAAAAACTATTATTTTTAGTTTTTAGAATTTCTTTAATATTTTTAAAATTTCAAAACAAAAACTAAAATTGGTTATCAAACAAACTTGTTTTTAAAAAAGTAAAAAAATAAAAAATAAAAAACCAAAAACGAAATGGTTATCAAACGAGACCTTAACTTTCTCTACAGTCATGAGTCCAATTTATTGATTTTTACTTTGATATCTACATTTTACCTAAACTCTAAAAAATTAAAGAGAAGAATTTGGGTGCACCCCGGGTAGCACCCCCACCATGACATCACTTATGTGGCATCTTTGGTGCCACACATTTTATCTCACTTTTTTTACTTTATATTTATTATAAAAAAAATTAAAAGAAAAAAGAAGATGTGTGGCTTCAAATGAATGATATCATGGTGGAGGTGCTACCCGGGGTGCACCTAAATTCTCCTCAAAATTAAATTGAAATTTGAAAATAAAGGTCGTGTTTGATAACTATTTGATTTTTTGTTTTTGAAATTTAAGCTTACTTTTACTCCAAATTCTTACAATATATTCCATCTTTTCTAGAGTGTATCTATCTTTTTCCTTTAGAAAAACTTTTGAAAGCTACCTATTTTTTCTTCCAAATTATGGCTTAGCTCCTAAAAATATAGATAAGACGTAGAAACTCAAGTAAGAAAAAACATAGGATAAGATAGATGTTGTAGGCCTAAATTTTAAAAATAAAAAATAAAATGGATATCAAACGGGACTTAAGAAACGTGCTTCTAAAACTTGTATTGGTTTTTAGAAGTTGGTTAAGATTCAAAACACTTTCTTAATGAAAAGAAAAGAAAAAAAGCTAAATTTTTTTTTTTTTTTAAATCAAATGGATGTTAATTTGTTTGGCGTGGCAGCTTGCAATGTTAAATGGTTAGGAGTGCGAGGAGATGATTTAGAAGTGATTCCACAACAATCTTTGGTTTCACTCAAACCCCGTGACCTACAAATTGCTAAAAGCTTGATGTCTTCACAAATATTACAGGTATTATTTATTTATTGTATTTTATTTTTTTAGGGTTTATCTAAATCCTAAGAATATTATTAATTAAACAACCCACAGGAGAATTATCGACAAGAGCTGGCCTTGATGGTTGAGAGAGGCCAAAAGGCTGAACTTGAAGCTTTGTACCACAATGGATTTGATTATTTGGAGAAGTATCTTGTCAAAAAGATTATTCAATTTAATTACATCTGACTTTATTTTTAACCTGC

mRNA sequence

TGAAGAACATCTTGAGCCAAACAAGGTGTTGGGCTTCATCACCTCACCTCACCTCATCTCCTCTTAAAACAAACGCCCCCTTAACATTGCAGATAACTTCCTCCAAGTAATTGAAGAATTTGAATATCTAACTTCCTCTTTTTCTCTGTTTTTTGAGTTTCTTTCTCTACAAATAGATCGCAACAGAACGTTTCAAAAATTCAAATACGCTATGGAGCAGACAGTGAGTTCATTCGAATCCTCCATGAGATTTTTCTCCGACCAAGAACTCTGCTACGCCGATCTCCTCACTCCTCAACAGGTTGGATCTTCGCTTCATTCGTAATCTCCGATCACTGTTCTTTCATCTCAAGCTCCGATTCCGAACAGGTGATTGCAAGAATCCAAGTCGCCGTTCTCAACTTCCTCACGATTCTCACTTCCTCCTCTCCTGCCATCTCGAATCTCCCTCTGATCGATCGACGATCGAGCAATAGCCGAGTGAATCGAGGCATTTTGACGGAAGAATCGTGTATTTTCCTTTCGAATTCGTTTTGTACGCGGTCTTTGATGCGATCTAATGCTGCGAAGGCTTTTGTTAGAGTGTGGAAGCTGATGGAGATTTGCTCTCAGATTCTGGGTCAGGAAAAGCGAGTCACACAGAGGGAGCTTTTCTACAAGTTGATATGTGATTCACCTGCCTATTTCCCAACTCAATTGCATGTCAATAGAACAATTCAAGATGTTGTAGCTTTACTTCGATGCAGCCGTTACAGCCTTGGAATTATGGCATCTAGTAGGGGACTTGTAGCTGGGCGTCTTTTGATTCAGGAGCCAGAGCAAGAAACTGTGGACTGCACTGCCTGTGGTTCCTCTGGATATGCCATTTCCGGCGACTTGGACCTGCTGCAGAAGTTGATTCTCAAGACCGACGCCCGGTACATAATTGTGATCGAAAAGCACGCAATATTCCAGCGGCTGGCGGAGGATTGTGTTTTCAACCACATTCCCAGTATTCTTATAACAGCCAAAGGATATCCAGACATAGCAACAAGGAACCCTGCTGGATTGGCTATATTGTGCACCTTCAAGTATGGAAGTATTGGGATGGGCTTGGAGGCATATAGATATGCTTGCAATGTTAAATGGTTAGGAGTGCGAGGAGATGATTTAGAAGTGATTCCACAACAATCTTTGGTTTCACTCAAACCCCGTGACCTACAAATTGCTAAAAGCTTGATGTCTTCACAAATATTACAGGAGAATTATCGACAAGAGCTGGCCTTGATGGTTGAGAGAGGCCAAAAGGCTGAACTTGAAGCTTTGTACCACAATGGATTTGATTATTTGGAGAAGTATCTTGTCAAAAAGATTATTCAATTTAATTACATCTGACTTTATTTTTAACCTGC

Coding sequence (CDS)

ATGCGATCTAATGCTGCGAAGGCTTTTGTTAGAGTGTGGAAGCTGATGGAGATTTGCTCTCAGATTCTGGGTCAGGAAAAGCGAGTCACACAGAGGGAGCTTTTCTACAAGTTGATATGTGATTCACCTGCCTATTTCCCAACTCAATTGCATGTCAATAGAACAATTCAAGATGTTGTAGCTTTACTTCGATGCAGCCGTTACAGCCTTGGAATTATGGCATCTAGTAGGGGACTTGTAGCTGGGCGTCTTTTGATTCAGGAGCCAGAGCAAGAAACTGTGGACTGCACTGCCTGTGGTTCCTCTGGATATGCCATTTCCGGCGACTTGGACCTGCTGCAGAAGTTGATTCTCAAGACCGACGCCCGGTACATAATTGTGATCGAAAAGCACGCAATATTCCAGCGGCTGGCGGAGGATTGTGTTTTCAACCACATTCCCAGTATTCTTATAACAGCCAAAGGATATCCAGACATAGCAACAAGGAACCCTGCTGGATTGGCTATATTGTGCACCTTCAAGTATGGAAGTATTGGGATGGGCTTGGAGGCATATAGATATGCTTGCAATGTTAAATGGTTAGGAGTGCGAGGAGATGATTTAGAAGTGATTCCACAACAATCTTTGGTTTCACTCAAACCCCGTGACCTACAAATTGCTAAAAGCTTGATGTCTTCACAAATATTACAGGAGAATTATCGACAAGAGCTGGCCTTGATGGTTGAGAGAGGCCAAAAGGCTGAACTTGAAGCTTTGTACCACAATGGATTTGATTATTTGGAGAAGTATCTTGTCAAAAAGATTATTCAATTTAATTACATCTGA

Protein sequence

MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQIAKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI

Homology

[Close]

BLAST of Sed0007632.5 vs. NCBI nr
Match: XP_023533742.1 (meiotic recombination protein SPO11-2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 503.4 bits (1295), Expect = 1.2e-138
Identity = 254/290 (87.59%), Postives = 268/290 (92.41%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL+CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLLCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACGSSGYAISGDLDLLQKL LKT
Sbjct: 150 ALLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGSSGYAISGDLDLLQKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR----------------NP 180
           DARY+IVIEKHAIFQRLAED VFNHIPSILITAKGYPDIATR                NP
Sbjct: 210 DARYVIVIEKHAIFQRLAEDRVFNHIPSILITAKGYPDIATRISKTFPHLPIFGLVDWNP 269

Query: 181 AGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQIAKSLM 240
           AGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQIAKSLM
Sbjct: 270 AGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQIAKSLM 329

Query: 241 SSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           SS+ILQENYR+EL LMVE GQKAELEALYHNGFDYLEKYLVKKIIQFNYI
Sbjct: 330 SSEILQENYREELTLMVEGGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 379

[Close]

BLAST of Sed0007632.5 vs. NCBI nr
Match: XP_023533741.1 (meiotic recombination protein SPO11-2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 501.5 bits (1290), Expect = 4.6e-138
Identity = 254/295 (86.10%), Postives = 268/295 (90.85%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL+CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLLCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACGSSGYAISGDLDLLQKL LKT
Sbjct: 150 ALLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGSSGYAISGDLDLLQKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR------------------ 180
           DARY+IVIEKHAIFQRLAED VFNHIPSILITAKGYPDIATR                  
Sbjct: 210 DARYVIVIEKHAIFQRLAEDRVFNHIPSILITAKGYPDIATRYFLHRISKTFPHLPIFGL 269

Query: 181 ---NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
              NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           AKSLMSS+ILQENYR+EL LMVE GQKAELEALYHNGFDYLEKYLVKKIIQFNYI
Sbjct: 330 AKSLMSSEILQENYREELTLMVEGGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 384

[Close]

BLAST of Sed0007632.5 vs. NCBI nr
Match: XP_022958585.1 (meiotic recombination protein SPO11-2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 498.4 bits (1282), Expect = 3.9e-137
Identity = 252/295 (85.42%), Postives = 267/295 (90.51%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLFCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACG+SGYAISGDLDLLQKL LKT
Sbjct: 150 ALLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGTSGYAISGDLDLLQKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR------------------ 180
           DARY+IVIEKHAIFQRLAED VFNHIPSILITAKGYPD+ATR                  
Sbjct: 210 DARYVIVIEKHAIFQRLAEDRVFNHIPSILITAKGYPDMATRYFLHRISKTFPHLPIFGL 269

Query: 181 ---NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
              NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           AKSLMSS+ILQENYR+EL LMVE GQKAELEALYHNGFDYLEKYLVKKIIQFNYI
Sbjct: 330 AKSLMSSEILQENYREELTLMVEGGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 384

[Close]

BLAST of Sed0007632.5 vs. NCBI nr
Match: KAG6606069.1 (Meiotic recombination protein SPO11-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 497.7 bits (1280), Expect = 6.6e-137
Identity = 251/295 (85.08%), Postives = 268/295 (90.85%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL+CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLLCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACG+SGYAISGDLDLL+KL LKT
Sbjct: 150 ALLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGTSGYAISGDLDLLRKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR------------------ 180
           DARY+IVIEKHAIFQRLAED VFNHIPSILITAKGYPD+ATR                  
Sbjct: 210 DARYVIVIEKHAIFQRLAEDRVFNHIPSILITAKGYPDMATRYFLHRISKTFPHLPIFGL 269

Query: 181 ---NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
              NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           AKSLMSS+ILQENYR+EL LMVE GQKAELEALYHNGFDYLEKYLVKKIIQFNYI
Sbjct: 330 AKSLMSSEILQENYREELTLMVEGGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 384

[Close]

BLAST of Sed0007632.5 vs. NCBI nr
Match: XP_022995805.1 (meiotic recombination protein SPO11-2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 496.5 bits (1277), Expect = 1.5e-136
Identity = 250/295 (84.75%), Postives = 266/295 (90.17%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL+CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLLCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
            LLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACGSSGYAISGDLDLLQKL LKT
Sbjct: 150 TLLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGSSGYAISGDLDLLQKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR------------------ 180
           DARY+IVIEKHAIFQRL ED VFNHIPSILITAKGYPDIATR                  
Sbjct: 210 DARYVIVIEKHAIFQRLVEDRVFNHIPSILITAKGYPDIATRYFLHRISKTFPHLPIFGL 269

Query: 181 ---NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
              NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           AKSLMSS+ILQENYR+EL LMVE GQKAELEALYHNGFDYL+KY+VKKIIQFNYI
Sbjct: 330 AKSLMSSEILQENYREELTLMVEGGQKAELEALYHNGFDYLQKYIVKKIIQFNYI 384

[Close]

BLAST of Sed0007632.5 vs. ExPASy Swiss-Prot
Match: Q9M4A1 (Meiotic recombination protein SPO11-2 OS=Arabidopsis thaliana OX=3702 GN=SPO11-2 PE=1 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 4.3e-115
Identity = 205/293 (69.97%), Postives = 243/293 (82.94%), Query Frame = 0


Query: 3   SNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVAL 62
           +  AKAFVRVWK+MEIC QIL QEKRVTQRELFYKL+CDSP YF +Q+ VNR++QDVVAL
Sbjct: 91  AKTAKAFVRVWKVMEICFQILLQEKRVTQRELFYKLLCDSPDYFSSQIEVNRSVQDVVAL 150

Query: 63  LRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDA 122
           LRCSRYSLGIMASSRGLVAGRL +QEP +E VDC+ACGSSG+AI+GDL+LL   I++TDA
Sbjct: 151 LRCSRYSLGIMASSRGLVAGRLFLQEPGKEAVDCSACGSSGFAITGDLNLLDNTIMRTDA 210

Query: 123 RYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR-------------------- 182
           RYII++EKHAIF RL ED VFNHIP + ITAKGYPDIATR                    
Sbjct: 211 RYIIIVEKHAIFHRLVEDRVFNHIPCVFITAKGYPDIATRFFLHRMSTTFPDLPILVLVD 270

Query: 183 -NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQIAK 242
            NPAGLAILCTFK+GSIGMGLEAYRYACNVKW+G+RGDDL +IP++SLV LKP+D QIAK
Sbjct: 271 WNPAGLAILCTFKFGSIGMGLEAYRYACNVKWIGLRGDDLNLIPEESLVPLKPKDSQIAK 330

Query: 243 SLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           SL+SS+ILQENY +EL+LMV+ G++AE+EALY +G++YL KY+  KI+Q  YI
Sbjct: 331 SLLSSKILQENYIEELSLMVQTGKRAEIEALYCHGYNYLGKYIATKIVQGKYI 383

[Close]

BLAST of Sed0007632.5 vs. ExPASy Swiss-Prot
Match: Q6ZD95 (Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-2 PE=2 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 4.5e-104
Identity = 187/294 (63.61%), Postives = 234/294 (79.59%), Query Frame = 0


Query: 2   RSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVA 61
           R+N AKAFVRVWK+ME+C +ILG+ K VT RELFY L+ +SP YF  Q HVN+T+QDVV+
Sbjct: 92  RANDAKAFVRVWKVMEMCYKILGEGKLVTLRELFYTLLSESPTYFTCQRHVNQTVQDVVS 151

Query: 62  LLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTD 121
           LLRC+R SLGIMASSRG + GRL++Q PE+E VDC+  G SG+AI+GDL++L KLI  +D
Sbjct: 152 LLRCTRQSLGIMASSRGALIGRLVVQGPEEEHVDCSILGPSGHAITGDLNVLSKLIFSSD 211

Query: 122 ARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR------------------- 181
           ARYIIV+EK AIFQRLAED +++H+P ILITAKGYPD+ATR                   
Sbjct: 212 ARYIIVVEKDAIFQRLAEDRIYSHLPCILITAKGYPDLATRFILHRLSQTYPNMPIFALV 271

Query: 182 --NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQIA 241
             NPAGLAILCT+KYGSI MGLE+YRYACNVKWLG+RGDDL++IPQ +   LKPRDLQIA
Sbjct: 272 DWNPAGLAILCTYKYGSISMGLESYRYACNVKWLGLRGDDLQLIPQSAYQELKPRDLQIA 331

Query: 242 KSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           KSL+SS+ LQ+ +R EL LM+E G++AE+EALY +GFD+L KY+ +KI+Q +YI
Sbjct: 332 KSLLSSKFLQDKHRAELTLMLETGKRAEIEALYSHGFDFLGKYVARKIVQGDYI 385

[Close]

BLAST of Sed0007632.5 vs. ExPASy Swiss-Prot
Match: Q5ZPI9 (Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. indica OX=39946 GN=SPO11-2 PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 5.8e-104
Identity = 187/294 (63.61%), Postives = 234/294 (79.59%), Query Frame = 0


Query: 2   RSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVA 61
           R+N AKAFVRVWK+ME+C +ILG+ K VT RELFY L+ +SP YF  Q HVN+T+QDVV+
Sbjct: 92  RANDAKAFVRVWKVMEMCYKILGEGKLVTLRELFYTLLSESPTYFTCQRHVNQTVQDVVS 151

Query: 62  LLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTD 121
           LLRC+R SLGIMASSRG + GRL++Q PE+E VDC+  G SG+AI+GDL++L KLI  +D
Sbjct: 152 LLRCTRQSLGIMASSRGALIGRLVLQGPEEEHVDCSILGPSGHAITGDLNVLSKLIFSSD 211

Query: 122 ARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR------------------- 181
           ARYIIV+EK AIFQRLAED +++H+P ILITAKGYPD+ATR                   
Sbjct: 212 ARYIIVVEKDAIFQRLAEDRIYSHLPCILITAKGYPDLATRFILHRLSQTYPNMPIFALV 271

Query: 182 --NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQIA 241
             NPAGLAILCT+KYGSI MGLE+YRYACNVKWLG+RGDDL++IPQ +   LKPRDLQIA
Sbjct: 272 DWNPAGLAILCTYKYGSISMGLESYRYACNVKWLGLRGDDLQLIPQSAYQELKPRDLQIA 331

Query: 242 KSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           KSL+SS+ LQ+ +R EL LM+E G++AE+EALY +GFD+L KY+ +KI+Q +YI
Sbjct: 332 KSLLSSKFLQDKHRAELTLMLETGKRAEIEALYSHGFDFLGKYVARKIVQGDYI 385

[Close]

BLAST of Sed0007632.5 vs. ExPASy Swiss-Prot
Match: Q9LZ03 (DNA topoisomerase 6 subunit A OS=Arabidopsis thaliana OX=3702 GN=TOP6A PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 5.8e-35
Identity = 94/287 (32.75%), Postives = 159/287 (55.40%), Query Frame = 0


Query: 14  KLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVALLRCSRYSLGIM 73
           +++ +  Q+  +   VT+R+LFY  +      F  Q   +  + DV  +L C+R SL ++
Sbjct: 148 RILALIHQLCLRNIHVTKRDLFYTDV----KLFQDQTQSDAVLDDVSCMLGCTRSSLNVI 207

Query: 74  ASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDARYIIVIEKHAI 133
           A+ +G+V GRL+  +   + +DCT  G  G AI  ++D +    +++DA +I+++EK A 
Sbjct: 208 AAEKGVVVGRLIFSD-NGDMIDCTKMGMGGKAIPPNIDRVGD--MQSDAMFILLVEKDAA 267

Query: 134 FQRLAEDCVFNHIPSILITAKGYPDIATR--------------------NPAGLAILCTF 193
           + RLAED  +N  P I++TAKG PD+ATR                    +P GL IL  +
Sbjct: 268 YMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPYGLKILSVY 327

Query: 194 KYGSIGMGLEAYRYAC-NVKWLGVRGDDLE--VIPQQSLVSLKPRDLQIAKSLMSSQILQ 253
             GS  M  ++      ++KWLG+R  DL+   IP+Q  + +  +D++  K ++    ++
Sbjct: 328 GCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDIKTGKDMLEEDFVK 387

Query: 254 EN--YRQELALMVERGQKAELEALYHNGFDYL-EKYLVKKIIQFNYI 275
           +N  + +EL LMV+  QKAE++AL   GF YL E YL  K+ Q +++
Sbjct: 388 KNPGWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQQDWL 427

[Close]

BLAST of Sed0007632.5 vs. ExPASy Swiss-Prot
Match: A2XFC1 (DNA topoisomerase 6 subunit A3 OS=Oryza sativa subsp. indica OX=39946 GN=TOP6A3 PE=1 SV=2)

HSP 1 Score: 149.4 bits (376), Expect = 5.8e-35
Identity = 99/298 (33.22%), Postives = 162/298 (54.36%), Query Frame = 0


Query: 3   SNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVAL 62
           + A KA +   +++ +   +L +   VT+R+LFY  +      F  Q   +  + DV  +
Sbjct: 153 ATARKATITA-RVLSLVHAVLRRGIHVTKRDLFYTDV----KLFGDQAQSDAVLDDVSCM 212

Query: 63  LRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDA 122
           L C+R SL ++AS +G+V GRL   + + + +DCT  G  G AI  ++D +    +++DA
Sbjct: 213 LGCTRSSLHVVASEKGVVVGRLTFAD-DGDRIDCTRMGVGGKAIPPNIDRVSG--IESDA 272

Query: 123 RYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR-------------------- 182
            +I+++EK A F RLAED  +N  P I++TAKG PD+ATR                    
Sbjct: 273 LFILLVEKDAAFMRLAEDRFYNRFPCIILTAKGQPDVATRLFLRRLKVELKLPVLALVDS 332

Query: 183 NPAGLAILCTFKYGSIGMGLEAYRYAC-NVKWLGVRGDDLEV--IPQQSLVSLKPRDLQI 242
           +P GL IL  +  GS  M  ++      ++KWLGVR  DL+   +P+Q  + +   D+++
Sbjct: 333 DPYGLKILSVYMCGSKNMSYDSANLTTPDIKWLGVRPSDLDKYRVPEQCRLPMTDHDIKV 392

Query: 243 AKSLMSSQILQEN--YRQELALMVERGQKAELEALYHNGFDYL-EKYLVKKIIQFNYI 275
            K L+    +++N  + +EL  M+   QKAE++AL   GF YL E YL  K+ Q ++I
Sbjct: 393 GKELLEEDFVKQNEGWVKELETMLRTRQKAEIQALSSFGFQYLTEVYLPLKLQQQDWI 442

[Close]

BLAST of Sed0007632.5 vs. ExPASy TrEMBL
Match: A0A6J1H3X3 (meiotic recombination protein SPO11-2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459771 PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.9e-137
Identity = 252/295 (85.42%), Postives = 267/295 (90.51%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLFCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACG+SGYAISGDLDLLQKL LKT
Sbjct: 150 ALLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGTSGYAISGDLDLLQKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR------------------ 180
           DARY+IVIEKHAIFQRLAED VFNHIPSILITAKGYPD+ATR                  
Sbjct: 210 DARYVIVIEKHAIFQRLAEDRVFNHIPSILITAKGYPDMATRYFLHRISKTFPHLPIFGL 269

Query: 181 ---NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
              NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           AKSLMSS+ILQENYR+EL LMVE GQKAELEALYHNGFDYLEKYLVKKIIQFNYI
Sbjct: 330 AKSLMSSEILQENYREELTLMVEGGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 384

[Close]

BLAST of Sed0007632.5 vs. ExPASy TrEMBL
Match: A0A6J1K4Y3 (meiotic recombination protein SPO11-2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491232 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 7.1e-137
Identity = 250/295 (84.75%), Postives = 266/295 (90.17%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL+CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLLCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
            LLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACGSSGYAISGDLDLLQKL LKT
Sbjct: 150 TLLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGSSGYAISGDLDLLQKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR------------------ 180
           DARY+IVIEKHAIFQRL ED VFNHIPSILITAKGYPDIATR                  
Sbjct: 210 DARYVIVIEKHAIFQRLVEDRVFNHIPSILITAKGYPDIATRYFLHRISKTFPHLPIFGL 269

Query: 181 ---NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
              NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           AKSLMSS+ILQENYR+EL LMVE GQKAELEALYHNGFDYL+KY+VKKIIQFNYI
Sbjct: 330 AKSLMSSEILQENYREELTLMVEGGQKAELEALYHNGFDYLQKYIVKKIIQFNYI 384

[Close]

BLAST of Sed0007632.5 vs. ExPASy TrEMBL
Match: A0A1S3AUC6 (meiotic recombination protein SPO11-2 OS=Cucumis melo OX=3656 GN=LOC103482788 PE=3 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 8.7e-135
Identity = 249/295 (84.41%), Postives = 264/295 (89.49%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR NA+KAFVRVWKLME+CSQIL Q+KRVTQRELFYKL+CDSPAYF TQLHVNRTIQDVV
Sbjct: 90  MRPNASKAFVRVWKLMEMCSQILIQDKRVTQRELFYKLLCDSPAYFSTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALL+CSRYSLGIMASSRGLVAGRLL+QEPEQE VDCTACGSSGY ISGDLDLL+ L LKT
Sbjct: 150 ALLQCSRYSLGIMASSRGLVAGRLLLQEPEQEVVDCTACGSSGYPISGDLDLLKMLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR------------------ 180
           DARYIIVIEKHAIFQRLA+D VFNHIPSILITAKGYPD+ATR                  
Sbjct: 210 DARYIIVIEKHAIFQRLADDRVFNHIPSILITAKGYPDLATRYFLHKIGKTFPHLPMFGL 269

Query: 181 ---NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
              NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDL++IPQ+SLV LKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLQLIPQESLVPLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           AKSLMSS+ILQENYRQEL LMVERGQKAELEALYHNGFDYLEKY+VKKIIQF+YI
Sbjct: 330 AKSLMSSKILQENYRQELTLMVERGQKAELEALYHNGFDYLEKYIVKKIIQFSYI 384

[Close]

BLAST of Sed0007632.5 vs. ExPASy TrEMBL
Match: A0A6J1DVQ2 (meiotic recombination protein SPO11-2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023944 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 9.6e-134
Identity = 244/295 (82.71%), Postives = 262/295 (88.81%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAA+AFVRVWKLME+CSQIL QEKRVTQRELFYKL+CDSPAYFPTQLHVNRTIQDVV
Sbjct: 62  MRANAAEAFVRVWKLMEMCSQILLQEKRVTQRELFYKLLCDSPAYFPTQLHVNRTIQDVV 121

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALLRCSRYSLGIMASSRGLVAGRLL+QEP  E VDC ACG SGYAISGDL+LLQKL LKT
Sbjct: 122 ALLRCSRYSLGIMASSRGLVAGRLLLQEPGDEVVDCAACGPSGYAISGDLNLLQKLTLKT 181

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR------------------ 180
           DARYIIV+EKHAIFQRL ED VFNH PSILITAKGYPDIATR                  
Sbjct: 182 DARYIIVVEKHAIFQRLLEDRVFNHFPSILITAKGYPDIATRYFLHRISEEFPNLPILGL 241

Query: 181 ---NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
              NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDL+++PQ+SLVSLKPRDLQI
Sbjct: 242 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLQLLPQESLVSLKPRDLQI 301

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           AKSLMSS+ LQ+NYR+ELALMV+RGQKAE+EALYHNGFDYLEKY+ KKI+QFNYI
Sbjct: 302 AKSLMSSETLQDNYREELALMVDRGQKAEIEALYHNGFDYLEKYIAKKIVQFNYI 356

[Close]

BLAST of Sed0007632.5 vs. ExPASy TrEMBL
Match: A0A6J1DSN2 (meiotic recombination protein SPO11-2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023944 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 9.6e-134
Identity = 244/295 (82.71%), Postives = 262/295 (88.81%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAA+AFVRVWKLME+CSQIL QEKRVTQRELFYKL+CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAEAFVRVWKLMEMCSQILLQEKRVTQRELFYKLLCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALLRCSRYSLGIMASSRGLVAGRLL+QEP  E VDC ACG SGYAISGDL+LLQKL LKT
Sbjct: 150 ALLRCSRYSLGIMASSRGLVAGRLLLQEPGDEVVDCAACGPSGYAISGDLNLLQKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR------------------ 180
           DARYIIV+EKHAIFQRL ED VFNH PSILITAKGYPDIATR                  
Sbjct: 210 DARYIIVVEKHAIFQRLLEDRVFNHFPSILITAKGYPDIATRYFLHRISEEFPNLPILGL 269

Query: 181 ---NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
              NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDL+++PQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLQLLPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           AKSLMSS+ LQ+NYR+ELALMV+RGQKAE+EALYHNGFDYLEKY+ KKI+QFNYI
Sbjct: 330 AKSLMSSETLQDNYREELALMVDRGQKAEIEALYHNGFDYLEKYIAKKIVQFNYI 384

[Close]

BLAST of Sed0007632.5 vs. TAIR 10
Match: AT1G63990.1 (sporulation 11-2 )

HSP 1 Score: 415.6 bits (1067), Expect = 3.1e-116
Identity = 205/293 (69.97%), Postives = 243/293 (82.94%), Query Frame = 0


Query: 3   SNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVAL 62
           +  AKAFVRVWK+MEIC QIL QEKRVTQRELFYKL+CDSP YF +Q+ VNR++QDVVAL
Sbjct: 91  AKTAKAFVRVWKVMEICFQILLQEKRVTQRELFYKLLCDSPDYFSSQIEVNRSVQDVVAL 150

Query: 63  LRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDA 122
           LRCSRYSLGIMASSRGLVAGRL +QEP +E VDC+ACGSSG+AI+GDL+LL   I++TDA
Sbjct: 151 LRCSRYSLGIMASSRGLVAGRLFLQEPGKEAVDCSACGSSGFAITGDLNLLDNTIMRTDA 210

Query: 123 RYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATR-------------------- 182
           RYII++EKHAIF RL ED VFNHIP + ITAKGYPDIATR                    
Sbjct: 211 RYIIIVEKHAIFHRLVEDRVFNHIPCVFITAKGYPDIATRFFLHRMSTTFPDLPILVLVD 270

Query: 183 -NPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQIAK 242
            NPAGLAILCTFK+GSIGMGLEAYRYACNVKW+G+RGDDL +IP++SLV LKP+D QIAK
Sbjct: 271 WNPAGLAILCTFKFGSIGMGLEAYRYACNVKWIGLRGDDLNLIPEESLVPLKPKDSQIAK 330

Query: 243 SLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 275
           SL+SS+ILQENY +EL+LMV+ G++AE+EALY +G++YL KY+  KI+Q  YI
Sbjct: 331 SLLSSKILQENYIEELSLMVQTGKRAEIEALYCHGYNYLGKYIATKIVQGKYI 383

[Close]

BLAST of Sed0007632.5 vs. TAIR 10
Match: AT5G02820.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 149.4 bits (376), Expect = 4.1e-36
Identity = 94/287 (32.75%), Postives = 159/287 (55.40%), Query Frame = 0


Query: 14  KLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVALLRCSRYSLGIM 73
           +++ +  Q+  +   VT+R+LFY  +      F  Q   +  + DV  +L C+R SL ++
Sbjct: 148 RILALIHQLCLRNIHVTKRDLFYTDV----KLFQDQTQSDAVLDDVSCMLGCTRSSLNVI 207

Query: 74  ASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDARYIIVIEKHAI 133
           A+ +G+V GRL+  +   + +DCT  G  G AI  ++D +    +++DA +I+++EK A 
Sbjct: 208 AAEKGVVVGRLIFSD-NGDMIDCTKMGMGGKAIPPNIDRVGD--MQSDAMFILLVEKDAA 267

Query: 134 FQRLAEDCVFNHIPSILITAKGYPDIATR--------------------NPAGLAILCTF 193
           + RLAED  +N  P I++TAKG PD+ATR                    +P GL IL  +
Sbjct: 268 YMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPYGLKILSVY 327

Query: 194 KYGSIGMGLEAYRYAC-NVKWLGVRGDDLE--VIPQQSLVSLKPRDLQIAKSLMSSQILQ 253
             GS  M  ++      ++KWLG+R  DL+   IP+Q  + +  +D++  K ++    ++
Sbjct: 328 GCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDIKTGKDMLEEDFVK 387

Query: 254 EN--YRQELALMVERGQKAELEALYHNGFDYL-EKYLVKKIIQFNYI 275
           +N  + +EL LMV+  QKAE++AL   GF YL E YL  K+ Q +++
Sbjct: 388 KNPGWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQQDWL 427

[Close]

BLAST of Sed0007632.5 vs. TAIR 10
Match: AT3G13170.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 125.6 bits (314), Expect = 6.3e-29
Identity = 88/283 (31.10%), Postives = 152/283 (53.71%), Query Frame = 0


Query: 14  KLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVALLRCSRYSLGIM 73
           +++ I  Q+L + +  ++R+++Y      P+ F  Q  V+R I D+  L +CSRY+L ++
Sbjct: 81  RVLLIVQQLLQENRHASKRDIYYM----HPSAFKAQSIVDRAIGDICILFQCSRYNLNVV 140

Query: 74  ASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDARYIIVIEKHAI 133
           +   GLV G L  +E  ++  DC    ++ Y +   ++ ++ ++  + A YI+V+EK  +
Sbjct: 141 SVGNGLVMGWLKFREAGRK-FDCLNSLNTAYPVPVLVEEVEDIV--SLAEYILVVEKETV 200

Query: 134 FQRLAEDCVFNHIPSILITAKGYPDIATR--------------------NPAGLAILCTF 193
           FQRLA D        I+IT +GYPD++TR                    +P G  IL T+
Sbjct: 201 FQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLPVHCLVDCDPYGFEILATY 260

Query: 194 KYGSIGMG--LEAYRYACNVKWLGVRGDDLEV--IPQQSLVSLKPRDLQIAKSLMSSQIL 253
           ++GS+ M   +E+ R A ++KWLG    D EV  +P+Q L+ L   D +  ++++    L
Sbjct: 261 RFGSMQMAYDIESLR-APDMKWLGAFPSDSEVYSVPKQCLLPLTEEDKKRTEAMLLRCYL 320

Query: 254 QE---NYRQELALMVERGQKAELEALYHNGFDYL-EKYLVKKI 269
           +     +R EL  M++RG K E+EAL  +   +L E Y+  KI
Sbjct: 321 KREMPQWRLELETMLKRGVKFEIEALSVHSLSFLSEVYIPSKI 355

The following BLAST results are available for this feature:

BLAST of Sed0007632.5 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023533742.1	1.2e-138	87.59	meiotic recombination protein SPO11-2 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023533741.1	4.6e-138	86.10	meiotic recombination protein SPO11-2 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022958585.1	3.9e-137	85.42	meiotic recombination protein SPO11-2 isoform X1 [Cucurbita moschata]	[more]
KAG6606069.1	6.6e-137	85.08	Meiotic recombination protein SPO11-2, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022995805.1	1.5e-136	84.75	meiotic recombination protein SPO11-2 isoform X1 [Cucurbita maxima]	[more]

BLAST of Sed0007632.5 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M4A1	4.3e-115	69.97	Meiotic recombination protein SPO11-2 OS=Arabidopsis thaliana OX=3702 GN=SPO11-2...	[more]
Q6ZD95	4.5e-104	63.61	Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q5ZPI9	5.8e-104	63.61	Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. indica OX=39946 GN=...	[more]
Q9LZ03	5.8e-35	32.75	DNA topoisomerase 6 subunit A OS=Arabidopsis thaliana OX=3702 GN=TOP6A PE=1 SV=1	[more]
A2XFC1	5.8e-35	33.22	DNA topoisomerase 6 subunit A3 OS=Oryza sativa subsp. indica OX=39946 GN=TOP6A3 ...	[more]

BLAST of Sed0007632.5 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H3X3	1.9e-137	85.42	meiotic recombination protein SPO11-2 isoform X1 OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1K4Y3	7.1e-137	84.75	meiotic recombination protein SPO11-2 isoform X1 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A1S3AUC6	8.7e-135	84.41	meiotic recombination protein SPO11-2 OS=Cucumis melo OX=3656 GN=LOC103482788 PE...	[more]
A0A6J1DVQ2	9.6e-134	82.71	meiotic recombination protein SPO11-2 isoform X2 OS=Momordica charantia OX=3673 ...	[more]
A0A6J1DSN2	9.6e-134	82.71	meiotic recombination protein SPO11-2 isoform X1 OS=Momordica charantia OX=3673 ...	[more]

BLAST of Sed0007632.5 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G63990.1	3.1e-116	69.97	sporulation 11-2	[more]
AT5G02820.1	4.1e-36	32.75	Spo11/DNA topoisomerase VI, subunit A protein	[more]
AT3G13170.1	6.3e-29	31.10	Spo11/DNA topoisomerase VI, subunit A protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002815	Spo11/DNA topoisomerase VI subunit A	PRINTS	PR01550	TOP6AFAMILY	coord: 149..170 score: 37.5 coord: 49..66 score: 27.43 coord: 125..142 score: 42.71 coord: 67..82 score: 45.31
IPR002815	Spo11/DNA topoisomerase VI subunit A	PANTHER	PTHR10848	MEIOTIC RECOMBINATION PROTEIN SPO11	coord: 163..273
IPR002815	Spo11/DNA topoisomerase VI subunit A	PANTHER	PTHR10848	MEIOTIC RECOMBINATION PROTEIN SPO11	coord: 2..163
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 1..75 e-value: 1.8E-16 score: 62.2
None	No IPR available	GENE3D	3.40.1360.10		coord: 76..163 e-value: 8.3E-24 score: 86.3 coord: 164..274 e-value: 2.3E-23 score: 84.9
None	No IPR available	PANTHER	PTHR10848:SF0	MEIOTIC RECOMBINATION PROTEIN SPO11	coord: 163..273 coord: 2..163
IPR013049	Spo11/DNA topoisomerase VI, subunit A, N-terminal	PFAM	PF04406	TP6A_N	coord: 7..72 e-value: 2.6E-15 score: 56.2
IPR034136	Topoisomerase 6 subunit A/Spo11, TOPRIM domain	CDD	cd00223	TOPRIM_TopoIIB_SPO	coord: 123..259 e-value: 4.73849E-45 score: 147.786
IPR036078	Spo11/DNA topoisomerase VI subunit A superfamily	SUPERFAMILY	56726	DNA topoisomerase IV, alpha subunit	coord: 6..274

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sed0007632	Sed0007632	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0007632.5-three_prime_utr	Sed0007632.5-three_prime_utr-LG09:8354747..8354763	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0007632.5-exon	Sed0007632.5-exon-LG09:8354747..8354898	exon
Sed0007632.5-exon	Sed0007632.5-exon-LG09:8354977..8355104	exon
Sed0007632.5-exon	Sed0007632.5-exon-LG09:8356352..8356428	exon
Sed0007632.5-exon	Sed0007632.5-exon-LG09:8356657..8356751	exon
Sed0007632.5-exon	Sed0007632.5-exon-LG09:8356856..8356984	exon
Sed0007632.5-exon	Sed0007632.5-exon-LG09:8357725..8357813	exon
Sed0007632.5-exon	Sed0007632.5-exon-LG09:8357908..8358045	exon
Sed0007632.5-exon	Sed0007632.5-exon-LG09:8358943..8359072	exon
Sed0007632.5-exon	Sed0007632.5-exon-LG09:8359180..8359632	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0007632.5-cds	Sed0007632.5-cds-LG09:8354764..8354898	CDS
Sed0007632.5-cds	Sed0007632.5-cds-LG09:8354977..8355104	CDS
Sed0007632.5-cds	Sed0007632.5-cds-LG09:8356352..8356428	CDS
Sed0007632.5-cds	Sed0007632.5-cds-LG09:8356657..8356751	CDS
Sed0007632.5-cds	Sed0007632.5-cds-LG09:8356856..8356984	CDS
Sed0007632.5-cds	Sed0007632.5-cds-LG09:8357725..8357813	CDS
Sed0007632.5-cds	Sed0007632.5-cds-LG09:8357908..8358045	CDS
Sed0007632.5-cds	Sed0007632.5-cds-LG09:8358943..8358976	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0007632.5-five_prime_utr	Sed0007632.5-five_prime_utr-LG09:8358977..8359072	five_prime_UTR
Sed0007632.5-five_prime_utr	Sed0007632.5-five_prime_utr-LG09:8359180..8359632	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sed0007632.5	Sed0007632.5-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006259	DNA metabolic process
biological_process	GO:0042138	meiotic DNA double-strand break formation
biological_process	GO:0051026	chiasma assembly
biological_process	GO:0009553	embryo sac development
biological_process	GO:0000706	meiotic DNA double-strand break processing
biological_process	GO:0009555	pollen development
biological_process	GO:0048316	seed development
cellular_component	GO:0005694	chromosome
cellular_component	GO:0000228	nuclear chromosome
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003918	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0046872	metal ion binding