Sed0007632.2 (mRNA) Chayote v1

Overview


Name	Sed0007632.2
Type	mRNA
Organism	Sechium edule (Chayote v1)
Description	meiotic recombination protein SPO11-2
Location	LG09: 8354747 .. 8359632 (-)
Sequence length	1454
RNA-Seq Expression	Sed0007632.2
Synteny	Sed0007632.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGAAGAACATCTTGAGCCAAACAAGGTGTTGGGCTTCATCACCTCACCTCACCTCATCTCCTCTTAAAACAAACGCCCCCTTAACATTGCAGATAACTTCCTCCAAGTAATTGAAGAATTTGAATATCTAACTTCCTCTTTTTCTCTGTTTTTTGAGTTTCTTTCTCTACAAATAGATCGCAACAGAACGTTTCAAAAATTCAAATACGCTATGGAGCAGACAGTGAGTTCATTCGAATCCTCCATGAGATTTTTCTCCGACCAAGAACTCTGCTACGCCGATCTCCTCACTCCTCAACAGGTTGGATCTTCGCTTCATTCGTAATCTCCGATCACTGTTCTTTCATCTCAAGCTCCGATTCCGAACAGGTGATTGCAAGAATCCAAGTCGCCGTTCTCAACTTCCTCACGATTCTCACTTCCTCCTCTCCTGCCATCTCGAATCTCCCTCTGGTACGATTTCTATCCAAGAAATGGCACGAAATGAGTTTTATGTACTTCGATTTCTTGCTCTTTCCGTTTTTGAAACCCTAGGTTGTTCTCTGTTCCTGATTGAACAGATCGATCGACGATCGAGCAATAGCCGAGTGAATCGAGGCATTTTGACGGAAGAATCGTGTATTTTCCTTTCGAATTCGTTTTGTACGCGGTCTTTGATGCGATCTAATGCTGCGAAGGCTTTTGTTAGAGGTAATGTAATCATCGAACCTTAATTCTCGGCGGTCAGGTTCTTCGAATGTTGAATTTGTTCGGAGTTGTTTGGATGGTGTTTTGTAGGAGGAAATTAGTTTGATGAGTTATTTTGAGTAGTTAGAATTTGAGAGTTGCAACCAAAAAGTTGTTTGGATGTTATTTTTACTTAGAGGAGTTAGAGACACAATAACCAGTTATTTGAATAGTTAAAAAAGAATTATTAAAAAAGACCGGTTAAGAATGAGTTATTTTGAATATTTAAAAATGAATGATTAAAAATGAGTGGTTAGAAATGAGGAGTTATTTTGAATAGTTAAAAATGAGTGATTAGAAATGAGGAGTTATTTTGAGTAGTTAGAAATTAGTTGTTAGAAATGAAAAGTTATTTTGAGTAGTTAGAAATTAGTTGTTAGAAATGAAAAGTTATTTTGAGTAATTAGAAATTAGTTGTTAAAAATGAGGACTTATTTTGAGTAGTTAGAAATGAATGATTAGAAATTAGTTCTTACAAAGGAAGAGTTATTTTAAGTAATTAGAAATGATGATTAGAAATTAGTTGTTAAAAATGAGGACTTATTTTGACTAATTAGAAATGAATGATTAGAAATGAAGAATTAGTTTTAGTAGTAAAAATAAGGAGTATTCTTGAGGGGTGGTGTAGTCCTTGAAGTCTTGGATTTTGAAAGTACACTCCCCTCAAGGTCCTAAGTTCGAGACTCACATGTGACAGTAATTTGTAGACACCTCCCGCGTCGTTATTCCTTTGATGTCTCCTCATGCCCTAGGCCGAAAAATAAGGAGTTGTTTTTGAAGTGGGGTTAGTAAATACTCATTTTAAATCCTCCATTAATTCAACAAACAAACACCCTTCTATTTTGGTCAATTGTGCTGTAGTGTGGAAGCTGATGGAGATTTGCTCTCAGATTCTGGGTCAGGAAAAGCGAGTCACACAGAGGGAGCTTTTCTACAAGTTGATATGTGATTCACCTGCCTATTTCCCAACTCAATTGCATGTCAATAGAACAATTCAAGGTGATTTTGTGTTCTATGAACAAACACTGGCTGGTTTAAGGCCTAAAATTACATTGAACATAGTGATGAAATTTGGGTAGTTTTGTTTTTGTAGATGTTGTAGCTTTACTTCGATGCAGCCGTTACAGCCTTGGAATTATGGCATCTAGTAGGGGACTTGTAGCTGGGCGTCTTTTGATTCAGGCACATTGCTATGTCTTTTATTTTTGGATCTTATATTGCAATATAAAAGTATTCAAAATTACATGATAACCAAGTCATTTGAAGAACTTGAATTGTCCTTAATCTTGAGATCACTTAAATACTTTTTGTTTTATATCTAGCTGAGCATTCGAATCAGCTAACATGCACCTTGTTTATTATCACTGAACAAATGGTCTGATCCTTCAACATTTGACTGTCAAATAAACTTGTATAAAATTAACTGAGGTAAATGGCCAACAAAGGAGTAGTACTCCAATAACATTATGTATAACATGGTAACCAGTTGATTACCGGTCGAGTCCCAAATGTGGTTGAATTCAAAATTATTAGGTAGGTGGTTACTAAAGATTGAACTCATAATTTCTTAAAAAGTACAAAGTCCTATTTGATCACTAGGCCGATGGTTAAAGTCTTAGAATACTTATTACTCAGTGAATAATTGCATACTTTCTTCTATATACAGCACCTCTATTTTATAAATTATAACTAAATGTCGAGTAAATTCTTATATCCTATCATATGCATGAGATGTAAATTGGCTAGTATTGAGTCCCTCTTTTTCTATTTTCACATCAATATTACTTTGAAACCATTGCCAATTGCTAATTTATGTATAGTTTTATTTCTTGGTATCTGGCCAGCACACACTTATAAGGTTTCTCATATTGAACCAAATTCTTGAATGTTGAATCCTTTATAAACAATTTCAATTGCTGTAGGAGCCAGAGCAAGAAACTGTGGACTGCACTGCCTGTGGTTCCTCTGGATATGCCATTTCCGGCGACTTGGACCTGCTGCAGAAGTTGATTCTCAAGACCGACGCCCGGTACATAATTGTGATCGAAAAGGTGATTTAAGTTTCTGAAGATATGATTTGCTAGTTAATTATACTTTTGTGAAGACAAAAAATGTGAAAAATGTAGTCAACTACCAAACATTGTTGGTTATACAGCACGCAATATTCCAGCGGCTGGCGGAGGATTGTGTTTTCAACCACATTCCCAGTATTCTTATAACAGCCAAAGGATATCCAGACATAGCAACAAGGTAAGTTTGTTCAACTAGTTTCGAAGATCGCAGTTCCTCGATGCGTTCTTGAAGCCGTAACTAAAACTCTGTGATCTGCCTTTTCAGGTATTTTCTTCACAAAATCAGCAAAATTTTCCCCCATCTGCCGATTTTCGGCCTTGTTGATTGGTATGATCCATTTTAACATCAACTCAGCAACAAGGTTGTAGAACTGTGTTCTCACTTTTAGCATTTGGGGTTCTAAAGGAACCCTGCTGGATTGGCTATATTGTGCACCTTCAAGTATGGAAGTATTGGGATGGGCTTGGAGGCATATAGATATGGTATGTCTCAGTTCCTTGATTGTTCTATCCATTTCTGATTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTAAGATGTTCAAACATTTGGATTTTGGTTTTTTTTCTTTTTTGAACTAAAGGATGTTTTATAGCCAAATTACAAAAAGGAAAACAAGTTTTTTATTTTTTGTTTTAAAAAGTAAAAACCATATATGTTTGATAACTCATTTTAGTTTTATGTTTTTAAAAAATGAAAAACCAAAAAACATGTTCGACAACTGTTTTTTGTTTTCAAATATCACATTTTATTTGGATTTTCTTAGTTTTTTACTATTTTTTCTTGTTCTTTTCCTACTTTTTTTTAAACAAAAACTTGTTTTTAAAAACTATTATTTTTAGTTTTTAGAATTTCTTTAATATTTTTAAAATTTCAAAACAAAAACTAAAATTGGTTATCAAACAAACTTGTTTTTAAAAAAGTAAAAAAATAAAAAATAAAAAACCAAAAACGAAATGGTTATCAAACGAGACCTTAACTTTCTCTACAGTCATGAGTCCAATTTATTGATTTTTACTTTGATATCTACATTTTACCTAAACTCTAAAAAATTAAAGAGAAGAATTTGGGTGCACCCCGGGTAGCACCCCCACCATGACATCACTTATGTGGCATCTTTGGTGCCACACATTTTATCTCACTTTTTTTACTTTATATTTATTATAAAAAAAATTAAAAGAAAAAAGAAGATGTGTGGCTTCAAATGAATGATATCATGGTGGAGGTGCTACCCGGGGTGCACCTAAATTCTCCTCAAAATTAAATTGAAATTTGAAAATAAAGGTCGTGTTTGATAACTATTTGATTTTTTGTTTTTGAAATTTAAGCTTACTTTTACTCCAAATTCTTACAATATATTCCATCTTTTCTAGAGTGTATCTATCTTTTTCCTTTAGAAAAACTTTTGAAAGCTACCTATTTTTTCTTCCAAATTATGGCTTAGCTCCTAAAAATATAGATAAGACGTAGAAACTCAAGTAAGAAAAAACATAGGATAAGATAGATGTTGTAGGCCTAAATTTTAAAAATAAAAAATAAAATGGATATCAAACGGGACTTAAGAAACGTGCTTCTAAAACTTGTATTGGTTTTTAGAAGTTGGTTAAGATTCAAAACACTTTCTTAATGAAAAGAAAAGAAAAAAAGCTAAATTTTTTTTTTTTTTTAAATCAAATGGATGTTAATTTGTTTGGCGTGGCAGCTTGCAATGTTAAATGGTTAGGAGTGCGAGGAGATGATTTAGAAGTGATTCCACAACAATCTTTGGTTTCACTCAAACCCCGTGACCTACAAATTGCTAAAAGCTTGATGTCTTCACAAATATTACAGGTATTATTTATTTATTGTATTTTATTTTTTTAGGGTTTATCTAAATCCTAAGAATATTATTAATTAAACAACCCACAGGAGAATTATCGACAAGAGCTGGCCTTGATGGTTGAGAGAGGCCAAAAGGCTGAACTTGAAGCTTTGTACCACAATGGATTTGATTATTTGGAGAAGTATCTTGTCAAAAAGATTATTCAATTTAATTACATCTGACTTTATTTTTAACCTGC

mRNA sequence

TGAAGAACATCTTGAGCCAAACAAGGTGTTGGGCTTCATCACCTCACCTCACCTCATCTCCTCTTAAAACAAACGCCCCCTTAACATTGCAGATAACTTCCTCCAAGTAATTGAAGAATTTGAATATCTAACTTCCTCTTTTTCTCTGTTTTTTGAGTTTCTTTCTCTACAAATAGATCGCAACAGAACGTTTCAAAAATTCAAATACGCTATGGAGCAGACAGTGAGTTCATTCGAATCCTCCATGAGATTTTTCTCCGACCAAGAACTCTGCTACGCCGATCTCCTCACTCCTCAACAGGTTGGATCTTCGCTTCATTCGTAATCTCCGATCACTGTTCTTTCATCTCAAGCTCCGATTCCGAACAGGTGATTGCAAGAATCCAAGTCGCCGTTCTCAACTTCCTCACGATTCTCACTTCCTCCTCTCCTGCCATCTCGAATCTCCCTCTGATCGATCGACGATCGAGCAATAGCCGAGTGAATCGAGGCATTTTGACGGAAGAATCGTGTATTTTCCTTTCGAATTCGTTTTGTACGCGGTCTTTGATGCGATCTAATGCTGCGAAGGCTTTTGTTAGAGTGTGGAAGCTGATGGAGATTTGCTCTCAGATTCTGGGTCAGGAAAAGCGAGTCACACAGAGGGAGCTTTTCTACAAGTTGATATGTGATTCACCTGCCTATTTCCCAACTCAATTGCATGTCAATAGAACAATTCAAGATGTTGTAGCTTTACTTCGATGCAGCCGTTACAGCCTTGGAATTATGGCATCTAGTAGGGGACTTGTAGCTGGGCGTCTTTTGATTCAGGAGCCAGAGCAAGAAACTGTGGACTGCACTGCCTGTGGTTCCTCTGGATATGCCATTTCCGGCGACTTGGACCTGCTGCAGAAGTTGATTCTCAAGACCGACGCCCGGTACATAATTGTGATCGAAAAGCACGCAATATTCCAGCGGCTGGCGGAGGATTGTGTTTTCAACCACATTCCCAGTATTCTTATAACAGCCAAAGGATATCCAGACATAGCAACAAGGTATTTTCTTCACAAAATCAGCAAAATTTTCCCCCATCTGCCGATTTTCGGCCTTGTTGATTGGAACCCTGCTGGATTGGCTATATTGTGCACCTTCAAGTATGGAAGTATTGGGATGGGCTTGGAGGCATATAGATATGCTTGCAATGTTAAATGGTTAGGAGTGCGAGGAGATGATTTAGAAGTGATTCCACAACAATCTTTGGTTTCACTCAAACCCCGTGACCTACAAATTGCTAAAAGCTTGATGTCTTCACAAATATTACAGGAGAATTATCGACAAGAGCTGGCCTTGATGGTTGAGAGAGGCCAAAAGGCTGAACTTGAAGCTTTGTACCACAATGGATTTGATTATTTGGAGAAGTATCTTGTCAAAAAGATTATTCAATTTAATTACATCTGACTTTATTTTTAACCTGC

Coding sequence (CDS)

ATGCGATCTAATGCTGCGAAGGCTTTTGTTAGAGTGTGGAAGCTGATGGAGATTTGCTCTCAGATTCTGGGTCAGGAAAAGCGAGTCACACAGAGGGAGCTTTTCTACAAGTTGATATGTGATTCACCTGCCTATTTCCCAACTCAATTGCATGTCAATAGAACAATTCAAGATGTTGTAGCTTTACTTCGATGCAGCCGTTACAGCCTTGGAATTATGGCATCTAGTAGGGGACTTGTAGCTGGGCGTCTTTTGATTCAGGAGCCAGAGCAAGAAACTGTGGACTGCACTGCCTGTGGTTCCTCTGGATATGCCATTTCCGGCGACTTGGACCTGCTGCAGAAGTTGATTCTCAAGACCGACGCCCGGTACATAATTGTGATCGAAAAGCACGCAATATTCCAGCGGCTGGCGGAGGATTGTGTTTTCAACCACATTCCCAGTATTCTTATAACAGCCAAAGGATATCCAGACATAGCAACAAGGTATTTTCTTCACAAAATCAGCAAAATTTTCCCCCATCTGCCGATTTTCGGCCTTGTTGATTGGAACCCTGCTGGATTGGCTATATTGTGCACCTTCAAGTATGGAAGTATTGGGATGGGCTTGGAGGCATATAGATATGCTTGCAATGTTAAATGGTTAGGAGTGCGAGGAGATGATTTAGAAGTGATTCCACAACAATCTTTGGTTTCACTCAAACCCCGTGACCTACAAATTGCTAAAAGCTTGATGTCTTCACAAATATTACAGGAGAATTATCGACAAGAGCTGGCCTTGATGGTTGAGAGAGGCCAAAAGGCTGAACTTGAAGCTTTGTACCACAATGGATTTGATTATTTGGAGAAGTATCTTGTCAAAAAGATTATTCAATTTAATTACATCTGA

Protein sequence

MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQIAKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI

Homology

[Close]

BLAST of Sed0007632.2 vs. NCBI nr
Match: XP_023533741.1 (meiotic recombination protein SPO11-2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 558.1 bits (1437), Expect = 4.4e-155
Identity = 273/295 (92.54%), Postives = 288/295 (97.63%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL+CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLLCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACGSSGYAISGDLDLLQKL LKT
Sbjct: 150 ALLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGSSGYAISGDLDLLQKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGL 180
           DARY+IVIEKHAIFQRLAED VFNHIPSILITAKGYPDIATRYFLH+ISK FPHLPIFGL
Sbjct: 210 DARYVIVIEKHAIFQRLAEDRVFNHIPSILITAKGYPDIATRYFLHRISKTFPHLPIFGL 269

Query: 181 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
           VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 296
           AKSLMSS+ILQENYR+EL LMVE GQKAELEALYHNGFDYLEKYLVKKIIQFNYI
Sbjct: 330 AKSLMSSEILQENYREELTLMVEGGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 384

[Close]

BLAST of Sed0007632.2 vs. NCBI nr
Match: XP_022958585.1 (meiotic recombination protein SPO11-2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 555.1 bits (1429), Expect = 3.7e-154
Identity = 271/295 (91.86%), Postives = 287/295 (97.29%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLFCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACG+SGYAISGDLDLLQKL LKT
Sbjct: 150 ALLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGTSGYAISGDLDLLQKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGL 180
           DARY+IVIEKHAIFQRLAED VFNHIPSILITAKGYPD+ATRYFLH+ISK FPHLPIFGL
Sbjct: 210 DARYVIVIEKHAIFQRLAEDRVFNHIPSILITAKGYPDMATRYFLHRISKTFPHLPIFGL 269

Query: 181 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
           VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 296
           AKSLMSS+ILQENYR+EL LMVE GQKAELEALYHNGFDYLEKYLVKKIIQFNYI
Sbjct: 330 AKSLMSSEILQENYREELTLMVEGGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 384

[Close]

BLAST of Sed0007632.2 vs. NCBI nr
Match: KAG6606069.1 (Meiotic recombination protein SPO11-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 554.3 bits (1427), Expect = 6.4e-154
Identity = 270/295 (91.53%), Postives = 288/295 (97.63%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL+CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLLCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACG+SGYAISGDLDLL+KL LKT
Sbjct: 150 ALLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGTSGYAISGDLDLLRKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGL 180
           DARY+IVIEKHAIFQRLAED VFNHIPSILITAKGYPD+ATRYFLH+ISK FPHLPIFGL
Sbjct: 210 DARYVIVIEKHAIFQRLAEDRVFNHIPSILITAKGYPDMATRYFLHRISKTFPHLPIFGL 269

Query: 181 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
           VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 296
           AKSLMSS+ILQENYR+EL LMVE GQKAELEALYHNGFDYLEKYLVKKIIQFNYI
Sbjct: 330 AKSLMSSEILQENYREELTLMVEGGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 384

[Close]

BLAST of Sed0007632.2 vs. NCBI nr
Match: XP_022995805.1 (meiotic recombination protein SPO11-2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 553.1 bits (1424), Expect = 1.4e-153
Identity = 269/295 (91.19%), Postives = 286/295 (96.95%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL+CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLLCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
            LLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACGSSGYAISGDLDLLQKL LKT
Sbjct: 150 TLLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGSSGYAISGDLDLLQKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGL 180
           DARY+IVIEKHAIFQRL ED VFNHIPSILITAKGYPDIATRYFLH+ISK FPHLPIFGL
Sbjct: 210 DARYVIVIEKHAIFQRLVEDRVFNHIPSILITAKGYPDIATRYFLHRISKTFPHLPIFGL 269

Query: 181 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
           VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 296
           AKSLMSS+ILQENYR+EL LMVE GQKAELEALYHNGFDYL+KY+VKKIIQFNYI
Sbjct: 330 AKSLMSSEILQENYREELTLMVEGGQKAELEALYHNGFDYLQKYIVKKIIQFNYI 384

[Close]

BLAST of Sed0007632.2 vs. NCBI nr
Match: XP_023533744.1 (meiotic recombination protein SPO11-2 isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 550.1 bits (1416), Expect = 1.2e-152
Identity = 273/305 (89.51%), Postives = 288/305 (94.43%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL+CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLLCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACGSSGYAISGDLDLLQKL LKT
Sbjct: 150 ALLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGSSGYAISGDLDLLQKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGL 180
           DARY+IVIEKHAIFQRLAED VFNHIPSILITAKGYPDIATRYFLH+ISK FPHLPIFGL
Sbjct: 210 DARYVIVIEKHAIFQRLAEDRVFNHIPSILITAKGYPDIATRYFLHRISKTFPHLPIFGL 269

Query: 181 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
           VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQ----------ENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKII 296
           AKSLMSS+ILQ          ENYR+EL LMVE GQKAELEALYHNGFDYLEKYLVKKII
Sbjct: 330 AKSLMSSEILQVSYNNPHLLMENYREELTLMVEGGQKAELEALYHNGFDYLEKYLVKKII 389

[Close]

BLAST of Sed0007632.2 vs. ExPASy Swiss-Prot
Match: Q9M4A1 (Meiotic recombination protein SPO11-2 OS=Arabidopsis thaliana OX=3702 GN=SPO11-2 PE=1 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 8.1e-128
Identity = 218/293 (74.40%), Postives = 259/293 (88.40%), Query Frame = 0


Query: 3   SNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVAL 62
           +  AKAFVRVWK+MEIC QIL QEKRVTQRELFYKL+CDSP YF +Q+ VNR++QDVVAL
Sbjct: 91  AKTAKAFVRVWKVMEICFQILLQEKRVTQRELFYKLLCDSPDYFSSQIEVNRSVQDVVAL 150

Query: 63  LRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDA 122
           LRCSRYSLGIMASSRGLVAGRL +QEP +E VDC+ACGSSG+AI+GDL+LL   I++TDA
Sbjct: 151 LRCSRYSLGIMASSRGLVAGRLFLQEPGKEAVDCSACGSSGFAITGDLNLLDNTIMRTDA 210

Query: 123 RYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGLVD 182
           RYII++EKHAIF RL ED VFNHIP + ITAKGYPDIATR+FLH++S  FP LPI  LVD
Sbjct: 211 RYIIIVEKHAIFHRLVEDRVFNHIPCVFITAKGYPDIATRFFLHRMSTTFPDLPILVLVD 270

Query: 183 WNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQIAK 242
           WNPAGLAILCTFK+GSIGMGLEAYRYACNVKW+G+RGDDL +IP++SLV LKP+D QIAK
Sbjct: 271 WNPAGLAILCTFKFGSIGMGLEAYRYACNVKWIGLRGDDLNLIPEESLVPLKPKDSQIAK 330

Query: 243 SLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 296
           SL+SS+ILQENY +EL+LMV+ G++AE+EALY +G++YL KY+  KI+Q  YI
Sbjct: 331 SLLSSKILQENYIEELSLMVQTGKRAEIEALYCHGYNYLGKYIATKIVQGKYI 383

[Close]

BLAST of Sed0007632.2 vs. ExPASy Swiss-Prot
Match: Q6ZD95 (Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-2 PE=2 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 3.8e-117
Identity = 198/294 (67.35%), Postives = 252/294 (85.71%), Query Frame = 0


Query: 2   RSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVA 61
           R+N AKAFVRVWK+ME+C +ILG+ K VT RELFY L+ +SP YF  Q HVN+T+QDVV+
Sbjct: 92  RANDAKAFVRVWKVMEMCYKILGEGKLVTLRELFYTLLSESPTYFTCQRHVNQTVQDVVS 151

Query: 62  LLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTD 121
           LLRC+R SLGIMASSRG + GRL++Q PE+E VDC+  G SG+AI+GDL++L KLI  +D
Sbjct: 152 LLRCTRQSLGIMASSRGALIGRLVVQGPEEEHVDCSILGPSGHAITGDLNVLSKLIFSSD 211

Query: 122 ARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGLV 181
           ARYIIV+EK AIFQRLAED +++H+P ILITAKGYPD+ATR+ LH++S+ +P++PIF LV
Sbjct: 212 ARYIIVVEKDAIFQRLAEDRIYSHLPCILITAKGYPDLATRFILHRLSQTYPNMPIFALV 271

Query: 182 DWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQIA 241
           DWNPAGLAILCT+KYGSI MGLE+YRYACNVKWLG+RGDDL++IPQ +   LKPRDLQIA
Sbjct: 272 DWNPAGLAILCTYKYGSISMGLESYRYACNVKWLGLRGDDLQLIPQSAYQELKPRDLQIA 331

Query: 242 KSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 296
           KSL+SS+ LQ+ +R EL LM+E G++AE+EALY +GFD+L KY+ +KI+Q +YI
Sbjct: 332 KSLLSSKFLQDKHRAELTLMLETGKRAEIEALYSHGFDFLGKYVARKIVQGDYI 385

[Close]

BLAST of Sed0007632.2 vs. ExPASy Swiss-Prot
Match: Q5ZPI9 (Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. indica OX=39946 GN=SPO11-2 PE=1 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 5.0e-117
Identity = 198/294 (67.35%), Postives = 252/294 (85.71%), Query Frame = 0


Query: 2   RSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVA 61
           R+N AKAFVRVWK+ME+C +ILG+ K VT RELFY L+ +SP YF  Q HVN+T+QDVV+
Sbjct: 92  RANDAKAFVRVWKVMEMCYKILGEGKLVTLRELFYTLLSESPTYFTCQRHVNQTVQDVVS 151

Query: 62  LLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTD 121
           LLRC+R SLGIMASSRG + GRL++Q PE+E VDC+  G SG+AI+GDL++L KLI  +D
Sbjct: 152 LLRCTRQSLGIMASSRGALIGRLVLQGPEEEHVDCSILGPSGHAITGDLNVLSKLIFSSD 211

Query: 122 ARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGLV 181
           ARYIIV+EK AIFQRLAED +++H+P ILITAKGYPD+ATR+ LH++S+ +P++PIF LV
Sbjct: 212 ARYIIVVEKDAIFQRLAEDRIYSHLPCILITAKGYPDLATRFILHRLSQTYPNMPIFALV 271

Query: 182 DWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQIA 241
           DWNPAGLAILCT+KYGSI MGLE+YRYACNVKWLG+RGDDL++IPQ +   LKPRDLQIA
Sbjct: 272 DWNPAGLAILCTYKYGSISMGLESYRYACNVKWLGLRGDDLQLIPQSAYQELKPRDLQIA 331

Query: 242 KSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 296
           KSL+SS+ LQ+ +R EL LM+E G++AE+EALY +GFD+L KY+ +KI+Q +YI
Sbjct: 332 KSLLSSKFLQDKHRAELTLMLETGKRAEIEALYSHGFDFLGKYVARKIVQGDYI 385

[Close]

BLAST of Sed0007632.2 vs. ExPASy Swiss-Prot
Match: A2XFC1 (DNA topoisomerase 6 subunit A3 OS=Oryza sativa subsp. indica OX=39946 GN=TOP6A3 PE=1 SV=2)

HSP 1 Score: 172.9 bits (437), Expect = 5.2e-42
Identity = 107/299 (35.79%), Postives = 174/299 (58.19%), Query Frame = 0


Query: 3   SNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVAL 62
           + A KA +   +++ +   +L +   VT+R+LFY  +      F  Q   +  + DV  +
Sbjct: 153 ATARKATITA-RVLSLVHAVLRRGIHVTKRDLFYTDV----KLFGDQAQSDAVLDDVSCM 212

Query: 63  LRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDA 122
           L C+R SL ++AS +G+V GRL   + + + +DCT  G  G AI  ++D +    +++DA
Sbjct: 213 LGCTRSSLHVVASEKGVVVGRLTFAD-DGDRIDCTRMGVGGKAIPPNIDRVSG--IESDA 272

Query: 123 RYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGLVD 182
            +I+++EK A F RLAED  +N  P I++TAKG PD+ATR FL ++ K+   LP+  LVD
Sbjct: 273 LFILLVEKDAAFMRLAEDRFYNRFPCIILTAKGQPDVATRLFLRRL-KVELKLPVLALVD 332

Query: 183 WNPAGLAILCTFKYGSIGMGLEAYRYAC-NVKWLGVRGDDLEV--IPQQSLVSLKPRDLQ 242
            +P GL IL  +  GS  M  ++      ++KWLGVR  DL+   +P+Q  + +   D++
Sbjct: 333 SDPYGLKILSVYMCGSKNMSYDSANLTTPDIKWLGVRPSDLDKYRVPEQCRLPMTDHDIK 392

Query: 243 IAKSLMSSQILQEN--YRQELALMVERGQKAELEALYHNGFDYL-EKYLVKKIIQFNYI 296
           + K L+    +++N  + +EL  M+   QKAE++AL   GF YL E YL  K+ Q ++I
Sbjct: 393 VGKELLEEDFVKQNEGWVKELETMLRTRQKAEIQALSSFGFQYLTEVYLPLKLQQQDWI 442

[Close]

BLAST of Sed0007632.2 vs. ExPASy Swiss-Prot
Match: Q0DSV9 (Topoisomerase 6 subunit A3 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-3 PE=2 SV=2)

HSP 1 Score: 172.9 bits (437), Expect = 5.2e-42
Identity = 107/299 (35.79%), Postives = 174/299 (58.19%), Query Frame = 0


Query: 3   SNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVAL 62
           + A KA +   +++ +   +L +   VT+R+LFY  +      F  Q   +  + DV  +
Sbjct: 153 ATARKATITA-RVLSLVHAVLRRGIHVTKRDLFYTDV----KLFGDQAQSDAVLDDVSCM 212

Query: 63  LRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDA 122
           L C+R SL ++AS +G+V GRL   + + + +DCT  G  G AI  ++D +    +++DA
Sbjct: 213 LGCTRSSLHVVASEKGVVVGRLTFAD-DGDRIDCTRMGVGGKAIPPNIDRVSG--IESDA 272

Query: 123 RYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGLVD 182
            +I+++EK A F RLAED  +N  P I++TAKG PD+ATR FL ++ K+   LP+  LVD
Sbjct: 273 LFILLVEKDAAFMRLAEDRFYNRFPCIILTAKGQPDVATRLFLRRL-KVELKLPVLALVD 332

Query: 183 WNPAGLAILCTFKYGSIGMGLEAYRYAC-NVKWLGVRGDDLEV--IPQQSLVSLKPRDLQ 242
            +P GL IL  +  GS  M  ++      ++KWLGVR  DL+   +P+Q  + +   D++
Sbjct: 333 SDPYGLKILSVYMCGSKNMSYDSANLTTPDIKWLGVRPSDLDKYRVPEQCRLPMTDHDIK 392

Query: 243 IAKSLMSSQILQEN--YRQELALMVERGQKAELEALYHNGFDYL-EKYLVKKIIQFNYI 296
           + K L+    +++N  + +EL  M+   QKAE++AL   GF YL E YL  K+ Q ++I
Sbjct: 393 VGKELLEEDFVKQNEGWVKELETMLRTRQKAEIQALSSFGFQYLTEVYLPLKLQQQDWI 442

[Close]

BLAST of Sed0007632.2 vs. ExPASy TrEMBL
Match: A0A6J1H3X3 (meiotic recombination protein SPO11-2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459771 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 1.8e-154
Identity = 271/295 (91.86%), Postives = 287/295 (97.29%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLFCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACG+SGYAISGDLDLLQKL LKT
Sbjct: 150 ALLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGTSGYAISGDLDLLQKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGL 180
           DARY+IVIEKHAIFQRLAED VFNHIPSILITAKGYPD+ATRYFLH+ISK FPHLPIFGL
Sbjct: 210 DARYVIVIEKHAIFQRLAEDRVFNHIPSILITAKGYPDMATRYFLHRISKTFPHLPIFGL 269

Query: 181 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
           VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 296
           AKSLMSS+ILQENYR+EL LMVE GQKAELEALYHNGFDYLEKYLVKKIIQFNYI
Sbjct: 330 AKSLMSSEILQENYREELTLMVEGGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 384

[Close]

BLAST of Sed0007632.2 vs. ExPASy TrEMBL
Match: A0A6J1K4Y3 (meiotic recombination protein SPO11-2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491232 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 6.9e-154
Identity = 269/295 (91.19%), Postives = 286/295 (96.95%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR+NAAKAFVRVWKLME+CSQIL QEKRVTQRELFYKL+CDSPAYFPTQLHVNRTIQDVV
Sbjct: 90  MRANAAKAFVRVWKLMEMCSQILLQEKRVTQRELFYKLLCDSPAYFPTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
            LLRCSR+SLG+MASSRGLVAGRLL+QEPEQETVDC ACGSSGYAISGDLDLLQKL LKT
Sbjct: 150 TLLRCSRFSLGVMASSRGLVAGRLLLQEPEQETVDCAACGSSGYAISGDLDLLQKLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGL 180
           DARY+IVIEKHAIFQRL ED VFNHIPSILITAKGYPDIATRYFLH+ISK FPHLPIFGL
Sbjct: 210 DARYVIVIEKHAIFQRLVEDRVFNHIPSILITAKGYPDIATRYFLHRISKTFPHLPIFGL 269

Query: 181 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
           VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLG+RGDD+++IPQ+SLVSLKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGMRGDDVQLIPQESLVSLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 296
           AKSLMSS+ILQENYR+EL LMVE GQKAELEALYHNGFDYL+KY+VKKIIQFNYI
Sbjct: 330 AKSLMSSEILQENYREELTLMVEGGQKAELEALYHNGFDYLQKYIVKKIIQFNYI 384

[Close]

BLAST of Sed0007632.2 vs. ExPASy TrEMBL
Match: A0A1S3AUC6 (meiotic recombination protein SPO11-2 OS=Cucumis melo OX=3656 GN=LOC103482788 PE=3 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 2.4e-151
Identity = 267/295 (90.51%), Postives = 283/295 (95.93%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR NA+KAFVRVWKLME+CSQIL Q+KRVTQRELFYKL+CDSPAYF TQLHVNRTIQDVV
Sbjct: 90  MRPNASKAFVRVWKLMEMCSQILIQDKRVTQRELFYKLLCDSPAYFSTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALL+CSRYSLGIMASSRGLVAGRLL+QEPEQE VDCTACGSSGY ISGDLDLL+ L LKT
Sbjct: 150 ALLQCSRYSLGIMASSRGLVAGRLLLQEPEQEVVDCTACGSSGYPISGDLDLLKMLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGL 180
           DARYIIVIEKHAIFQRLA+D VFNHIPSILITAKGYPD+ATRYFLHKI K FPHLP+FGL
Sbjct: 210 DARYIIVIEKHAIFQRLADDRVFNHIPSILITAKGYPDLATRYFLHKIGKTFPHLPMFGL 269

Query: 181 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
           VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDL++IPQ+SLV LKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLQLIPQESLVPLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 296
           AKSLMSS+ILQENYRQEL LMVERGQKAELEALYHNGFDYLEKY+VKKIIQF+YI
Sbjct: 330 AKSLMSSKILQENYRQELTLMVERGQKAELEALYHNGFDYLEKYIVKKIIQFSYI 384

[Close]

BLAST of Sed0007632.2 vs. ExPASy TrEMBL
Match: A0A5A7TMD5 (Meiotic recombination protein SPO11-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002190 PE=3 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.1e-148
Identity = 266/305 (87.21%), Postives = 283/305 (92.79%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR NA+KAFVRVWKLME+CSQIL Q+KRVTQRELFYKL+CDSPAYF TQLHVNRTIQDVV
Sbjct: 90  MRPNASKAFVRVWKLMEMCSQILIQDKRVTQRELFYKLLCDSPAYFSTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQ----------EPEQETVDCTACGSSGYAISGDL 120
           ALL+CSRYSLGIMASSRGLVAGRLL+Q          EPEQE VDCTACGSSGY ISGDL
Sbjct: 150 ALLQCSRYSLGIMASSRGLVAGRLLLQAHCTAFLIGLEPEQEVVDCTACGSSGYPISGDL 209

Query: 121 DLLQKLILKTDARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISK 180
           DLL+ L LKTDARYIIVIEKHAIFQRLA+D VFNHIPSILITAKGYPD+ATRYFLHKI K
Sbjct: 210 DLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILITAKGYPDLATRYFLHKIGK 269

Query: 181 IFPHLPIFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSL 240
            FPHLP+FGLVDWNPAG+AILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDL++IPQ+SL
Sbjct: 270 TFPHLPMFGLVDWNPAGMAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLQLIPQESL 329

Query: 241 VSLKPRDLQIAKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKII 296
           V LKPRDLQIAKSLMSS+ILQENYRQEL LMVERGQKAELEALYHNGFDYLEKY+VKKII
Sbjct: 330 VPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELEALYHNGFDYLEKYIVKKII 389

[Close]

BLAST of Sed0007632.2 vs. ExPASy TrEMBL
Match: A0A0A0KNU2 (TP6A_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152220 PE=3 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.1e-148
Identity = 263/295 (89.15%), Postives = 280/295 (94.92%), Query Frame = 0


Query: 1   MRSNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVV 60
           MR NA+KAFVRVWKLME+CSQIL Q+K+VTQRELFYKL+CDSP YF TQLHVNRTIQDVV
Sbjct: 90  MRPNASKAFVRVWKLMEMCSQILIQDKKVTQRELFYKLLCDSPVYFSTQLHVNRTIQDVV 149

Query: 61  ALLRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKT 120
           ALL+CSRYSLGIMASSRGLVAGRLL+QEPEQE VDCTACGSSGY ISGDLDLLQ L LKT
Sbjct: 150 ALLQCSRYSLGIMASSRGLVAGRLLLQEPEQEVVDCTACGSSGYPISGDLDLLQTLTLKT 209

Query: 121 DARYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGL 180
           DARYIIVIEKHAIFQRLA+D VFN IPSILITAKGYPD+ATRYFLHKI K F HLP+FGL
Sbjct: 210 DARYIIVIEKHAIFQRLADDRVFNRIPSILITAKGYPDLATRYFLHKICKTFSHLPMFGL 269

Query: 181 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQI 240
           VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDL++IPQ+SLV LKPRDLQI
Sbjct: 270 VDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLQLIPQESLVPLKPRDLQI 329

Query: 241 AKSLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 296
           AKSL+SS+ILQENYRQEL LMVERGQKAELEALYHNGFDYLEKY+VKKIIQF+YI
Sbjct: 330 AKSLLSSKILQENYRQELTLMVERGQKAELEALYHNGFDYLEKYIVKKIIQFSYI 384

[Close]

BLAST of Sed0007632.2 vs. TAIR 10
Match: AT1G63990.1 (sporulation 11-2 )

HSP 1 Score: 458.0 bits (1177), Expect = 5.8e-129
Identity = 218/293 (74.40%), Postives = 259/293 (88.40%), Query Frame = 0


Query: 3   SNAAKAFVRVWKLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVAL 62
           +  AKAFVRVWK+MEIC QIL QEKRVTQRELFYKL+CDSP YF +Q+ VNR++QDVVAL
Sbjct: 91  AKTAKAFVRVWKVMEICFQILLQEKRVTQRELFYKLLCDSPDYFSSQIEVNRSVQDVVAL 150

Query: 63  LRCSRYSLGIMASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDA 122
           LRCSRYSLGIMASSRGLVAGRL +QEP +E VDC+ACGSSG+AI+GDL+LL   I++TDA
Sbjct: 151 LRCSRYSLGIMASSRGLVAGRLFLQEPGKEAVDCSACGSSGFAITGDLNLLDNTIMRTDA 210

Query: 123 RYIIVIEKHAIFQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGLVD 182
           RYII++EKHAIF RL ED VFNHIP + ITAKGYPDIATR+FLH++S  FP LPI  LVD
Sbjct: 211 RYIIIVEKHAIFHRLVEDRVFNHIPCVFITAKGYPDIATRFFLHRMSTTFPDLPILVLVD 270

Query: 183 WNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLEVIPQQSLVSLKPRDLQIAK 242
           WNPAGLAILCTFK+GSIGMGLEAYRYACNVKW+G+RGDDL +IP++SLV LKP+D QIAK
Sbjct: 271 WNPAGLAILCTFKFGSIGMGLEAYRYACNVKWIGLRGDDLNLIPEESLVPLKPKDSQIAK 330

Query: 243 SLMSSQILQENYRQELALMVERGQKAELEALYHNGFDYLEKYLVKKIIQFNYI 296
           SL+SS+ILQENY +EL+LMV+ G++AE+EALY +G++YL KY+  KI+Q  YI
Sbjct: 331 SLLSSKILQENYIEELSLMVQTGKRAEIEALYCHGYNYLGKYIATKIVQGKYI 383

[Close]

BLAST of Sed0007632.2 vs. TAIR 10
Match: AT5G02820.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 172.6 bits (436), Expect = 4.9e-43
Identity = 103/288 (35.76%), Postives = 171/288 (59.38%), Query Frame = 0


Query: 14  KLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVALLRCSRYSLGIM 73
           +++ +  Q+  +   VT+R+LFY  +      F  Q   +  + DV  +L C+R SL ++
Sbjct: 148 RILALIHQLCLRNIHVTKRDLFYTDV----KLFQDQTQSDAVLDDVSCMLGCTRSSLNVI 207

Query: 74  ASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDARYIIVIEKHAI 133
           A+ +G+V GRL+  +   + +DCT  G  G AI  ++D +    +++DA +I+++EK A 
Sbjct: 208 AAEKGVVVGRLIFSD-NGDMIDCTKMGMGGKAIPPNIDRVGD--MQSDAMFILLVEKDAA 267

Query: 134 FQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGLVDWNPAGLAILCT 193
           + RLAED  +N  P I++TAKG PD+ATR FL K+ K+   LP+  LVD +P GL IL  
Sbjct: 268 YMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKM-KMELKLPVLALVDSDPYGLKILSV 327

Query: 194 FKYGSIGMGLEAYRYAC-NVKWLGVRGDDLE--VIPQQSLVSLKPRDLQIAKSLMSSQIL 253
           +  GS  M  ++      ++KWLG+R  DL+   IP+Q  + +  +D++  K ++    +
Sbjct: 328 YGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDIKTGKDMLEEDFV 387

Query: 254 QEN--YRQELALMVERGQKAELEALYHNGFDYL-EKYLVKKIIQFNYI 296
           ++N  + +EL LMV+  QKAE++AL   GF YL E YL  K+ Q +++
Sbjct: 388 KKNPGWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQQDWL 427

[Close]

BLAST of Sed0007632.2 vs. TAIR 10
Match: AT3G13170.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 147.5 bits (371), Expect = 1.7e-35
Identity = 96/284 (33.80%), Postives = 163/284 (57.39%), Query Frame = 0


Query: 14  KLMEICSQILGQEKRVTQRELFYKLICDSPAYFPTQLHVNRTIQDVVALLRCSRYSLGIM 73
           +++ I  Q+L + +  ++R+++Y      P+ F  Q  V+R I D+  L +CSRY+L ++
Sbjct: 81  RVLLIVQQLLQENRHASKRDIYYM----HPSAFKAQSIVDRAIGDICILFQCSRYNLNVV 140

Query: 74  ASSRGLVAGRLLIQEPEQETVDCTACGSSGYAISGDLDLLQKLILKTDARYIIVIEKHAI 133
           +   GLV G L  +E  ++  DC    ++ Y +   ++ ++ ++  + A YI+V+EK  +
Sbjct: 141 SVGNGLVMGWLKFREAGRK-FDCLNSLNTAYPVPVLVEEVEDIV--SLAEYILVVEKETV 200

Query: 134 FQRLAEDCVFNHIPSILITAKGYPDIATRYFLHKISKIFPHLPIFGLVDWNPAGLAILCT 193
           FQRLA D        I+IT +GYPD++TR FL  + +   HLP+  LVD +P G  IL T
Sbjct: 201 FQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKL-HLPVHCLVDCDPYGFEILAT 260

Query: 194 FKYGSIGMG--LEAYRYACNVKWLGVRGDDLEV--IPQQSLVSLKPRDLQIAKSLMSSQI 253
           +++GS+ M   +E+ R A ++KWLG    D EV  +P+Q L+ L   D +  ++++    
Sbjct: 261 YRFGSMQMAYDIESLR-APDMKWLGAFPSDSEVYSVPKQCLLPLTEEDKKRTEAMLLRCY 320

Query: 254 LQE---NYRQELALMVERGQKAELEALYHNGFDYL-EKYLVKKI 290
           L+     +R EL  M++RG K E+EAL  +   +L E Y+  KI
Sbjct: 321 LKREMPQWRLELETMLKRGVKFEIEALSVHSLSFLSEVYIPSKI 355

The following BLAST results are available for this feature:

BLAST of Sed0007632.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023533741.1	4.4e-155	92.54	meiotic recombination protein SPO11-2 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022958585.1	3.7e-154	91.86	meiotic recombination protein SPO11-2 isoform X1 [Cucurbita moschata]	[more]
KAG6606069.1	6.4e-154	91.53	Meiotic recombination protein SPO11-2, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022995805.1	1.4e-153	91.19	meiotic recombination protein SPO11-2 isoform X1 [Cucurbita maxima]	[more]
XP_023533744.1	1.2e-152	89.51	meiotic recombination protein SPO11-2 isoform X4 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sed0007632.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M4A1	8.1e-128	74.40	Meiotic recombination protein SPO11-2 OS=Arabidopsis thaliana OX=3702 GN=SPO11-2...	[more]
Q6ZD95	3.8e-117	67.35	Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q5ZPI9	5.0e-117	67.35	Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. indica OX=39946 GN=...	[more]
A2XFC1	5.2e-42	35.79	DNA topoisomerase 6 subunit A3 OS=Oryza sativa subsp. indica OX=39946 GN=TOP6A3 ...	[more]
Q0DSV9	5.2e-42	35.79	Topoisomerase 6 subunit A3 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-3 P...	[more]

BLAST of Sed0007632.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H3X3	1.8e-154	91.86	meiotic recombination protein SPO11-2 isoform X1 OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1K4Y3	6.9e-154	91.19	meiotic recombination protein SPO11-2 isoform X1 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A1S3AUC6	2.4e-151	90.51	meiotic recombination protein SPO11-2 OS=Cucumis melo OX=3656 GN=LOC103482788 PE...	[more]
A0A5A7TMD5	1.1e-148	87.21	Meiotic recombination protein SPO11-2 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A0A0KNU2	1.1e-148	89.15	TP6A_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152220 PE=3...	[more]

BLAST of Sed0007632.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G63990.1	5.8e-129	74.40	sporulation 11-2	[more]
AT5G02820.1	4.9e-43	35.76	Spo11/DNA topoisomerase VI, subunit A protein	[more]
AT3G13170.1	1.7e-35	33.80	Spo11/DNA topoisomerase VI, subunit A protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002815	Spo11/DNA topoisomerase VI subunit A	PRINTS	PR01550	TOP6AFAMILY	coord: 182..198 score: 48.53 coord: 125..142 score: 42.71 coord: 67..82 score: 45.31 coord: 149..170 score: 44.03 coord: 49..66 score: 27.43
IPR002815	Spo11/DNA topoisomerase VI subunit A	PANTHER	PTHR10848	MEIOTIC RECOMBINATION PROTEIN SPO11	coord: 2..294
IPR013049	Spo11/DNA topoisomerase VI, subunit A, N-terminal	PFAM	PF04406	TP6A_N	coord: 7..72 e-value: 2.9E-15 score: 56.0
None	No IPR available	GENE3D	3.40.1360.10		coord: 76..295 e-value: 1.3E-65 score: 223.1
None	No IPR available	PANTHER	PTHR10848:SF0	MEIOTIC RECOMBINATION PROTEIN SPO11	coord: 2..294
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 1..75 e-value: 2.0E-16 score: 62.0
IPR034136	Topoisomerase 6 subunit A/Spo11, TOPRIM domain	CDD	cd00223	TOPRIM_TopoIIB_SPO	coord: 123..280 e-value: 6.09004E-57 score: 178.602
IPR036078	Spo11/DNA topoisomerase VI subunit A superfamily	SUPERFAMILY	56726	DNA topoisomerase IV, alpha subunit	coord: 6..295

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sed0007632	Sed0007632	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0007632.2-three_prime_utr	Sed0007632.2-three_prime_utr-LG09:8354747..8354763	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0007632.2-exon	Sed0007632.2-exon-LG09:8354747..8354898	exon
Sed0007632.2-exon	Sed0007632.2-exon-LG09:8354977..8355104	exon
Sed0007632.2-exon	Sed0007632.2-exon-LG09:8356352..8356428	exon
Sed0007632.2-exon	Sed0007632.2-exon-LG09:8356507..8356569	exon
Sed0007632.2-exon	Sed0007632.2-exon-LG09:8356657..8356751	exon
Sed0007632.2-exon	Sed0007632.2-exon-LG09:8356856..8356984	exon
Sed0007632.2-exon	Sed0007632.2-exon-LG09:8357725..8357813	exon
Sed0007632.2-exon	Sed0007632.2-exon-LG09:8357908..8358045	exon
Sed0007632.2-exon	Sed0007632.2-exon-LG09:8358943..8359072	exon
Sed0007632.2-exon	Sed0007632.2-exon-LG09:8359180..8359632	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0007632.2-cds	Sed0007632.2-cds-LG09:8354764..8354898	CDS
Sed0007632.2-cds	Sed0007632.2-cds-LG09:8354977..8355104	CDS
Sed0007632.2-cds	Sed0007632.2-cds-LG09:8356352..8356428	CDS
Sed0007632.2-cds	Sed0007632.2-cds-LG09:8356507..8356569	CDS
Sed0007632.2-cds	Sed0007632.2-cds-LG09:8356657..8356751	CDS
Sed0007632.2-cds	Sed0007632.2-cds-LG09:8356856..8356984	CDS
Sed0007632.2-cds	Sed0007632.2-cds-LG09:8357725..8357813	CDS
Sed0007632.2-cds	Sed0007632.2-cds-LG09:8357908..8358045	CDS
Sed0007632.2-cds	Sed0007632.2-cds-LG09:8358943..8358976	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0007632.2-five_prime_utr	Sed0007632.2-five_prime_utr-LG09:8358977..8359072	five_prime_UTR
Sed0007632.2-five_prime_utr	Sed0007632.2-five_prime_utr-LG09:8359180..8359632	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sed0007632.2	Sed0007632.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006259	DNA metabolic process
biological_process	GO:0042138	meiotic DNA double-strand break formation
biological_process	GO:0051026	chiasma assembly
biological_process	GO:0009553	embryo sac development
biological_process	GO:0000706	meiotic DNA double-strand break processing
biological_process	GO:0009555	pollen development
biological_process	GO:0048316	seed development
cellular_component	GO:0005694	chromosome
cellular_component	GO:0000228	nuclear chromosome
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003918	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0046872	metal ion binding