Sed0002925.2 (mRNA) Chayote v1

Overview
NameSed0002925.2
TypemRNA
OrganismSechium edule (Chayote v1)
DescriptionDiacylglycerol O-acyltransferase
LocationLG04: 43727515 .. 43732030 (+)
Sequence length1631
RNA-Seq ExpressionSed0002925.2
SyntenySed0002925.2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTACCACATAAGCTAAAGCAACTTTGGATTATTGGTAATAATCAAAGCCTCTTTTTCTTCTTTATCTATTTCTTACTTTTTCTCAACAGAAATAAATTTGTACATAAACTTTTTAGGCAGCGAAAGATATATTTGGACAAAATAATGGAGATTTTGGAAGATTCAGCAACAGCGACCCCCATGAGCCCTTTCAGCCAATATTTCAACACTAGTGAAATTTGTGTGTCTGTTTTGGGAGTAATCGAACTCGAATCTCCAATTACTCACTCCGAAGCTTTGTCAATTGTCACTGATGTTCTCATCCCTGCCAATCCACGTTTCACTTCGATCATGGTTCTCTTTCTCACTCCTTTCTGACTAAAATGATTAAATTTATCGCAACTCATTAACTTATGTTTTAACATAGTTTCTTGATAATGATAATTTGTTGCGCTTTATGAGCTAACCTTTAAGCCCACAAGTAATTACTAAACTAACATTTTTTTGAAATAATTTACATTCTTAAATTATGAAATTTGTTATGATCATAATCTTACCATTTGAGTTTTGACCCATCAACTCATAAACTTTGTAAATGTTGTAGTTATACAATTTTTTGGTTGATTGTTTGTTAAACGATGTTTATCAATTCAATGAACATCCAATCTCAACTTTCTATCCATTTAATAAGTTAAATAAATTATAGTTGATTCACATACTTGTCCATTTTTTTCATTGGGCTAGCACTCATCCTTAATGGGGGTGGGGAATCCCCTTTACATAGTGAATGAGAAAGTGAACAGGAGAAAAAAATTCCCGTGAGCTAAATGGGGATGGAGATGGAGATGGAGAGTATATTCTCCATTCCTATTTTCTCAATCTCCACCATGGTTATATATATATTTTTTATTATTTTAAAAAATATTTAATGAATATATATGATTTTATGATTAAAAAAAAATTATTGTTAATGATTTAGACAATTTGATATAAAATTCTTACTAAAATGTTTACGAATTTAAAATTTTTAATTGTGATCAATCAAAGGAAAATATTATGTCTACCGGAAGATTTTTTTTTTTTTTTGGAAATTTATTCAACAGTAAATTAAATTGATTATTGAATTCAAGAACTATTTTGTGAAATTAATCGGTATGTTTTCAATTTTTTAAAGCCATTGTATTGTTGGTGTATATTTATTTTTTTGGTATTGAAATGTTGTGATTGTTCATGACATTTTCTTTTGTAAACGGTATCATATATATAAACTTCACATTTTTCTTTTGACATTGTAATATATCAAGTTTAACAAGAAGAAAAAGAGACTGGGAAATAATTTTCCGTGAGAAACTTGATACCCACAAATACCCTGCAGGGAATCTCCACCCTCATTCATGCAAAATTTAATGGTGAATTTTCTAGGAATAGTAATTAAAATGTGTGGCCGAGATGGTAAAGTCATCTATGTCCCTGCACCGCTTCATGAACATCTATAATACTTAATTAATATAAAAAGTTTGATTACAACACACTTGATATGATTTACAAGTTAATTTAAAAACATAAATGCAATAAGTAAAAAAAAAATTATGGATTAAAATTAAAAGTTGATCGATAACTTTGGTGGGTGATAAACTCTAATGCAATTTTCAAGAATTTATTTCTTTTAAAAGAAATAAATTCTTGAAAATTGGAGATATATGGCACAAACAATAACCTAAATAATAGATAAATATGTTTCAAAAAAAAAAAAGATAATAATAAATAAGTATGGATATTTAGGTGTATGTGATTAAATATTTAAGTGGAGCCTCCGGTCCAAGTTTCTGGTAAGGGAGTGGTTGCTATTGAATATGGAAGAATAAATATACTAAATTAGCTTTAAATACAACTTGGTAGTTAGAAACATTTATTCGACCATTTGTCATATTAAAAAAATATATAGGTATAAAAGATTAAAGTACTTATGGTAAGTTATGAACCAGAATGTTGTGGATCTTGAAAATGGACATGCAGGTGAAAGAAAAGTGTTGGGAAGAAAGAAAATGGAAGGCAACTAAGGTTAACCTAGAAGACCACATATACACACCAAAATTTCCCATGAATCTCTCCCTTTATGAATATGATGCTTACTTTGATGAATATGCAACCAAAACAGCTACAAAACCCTTTTCACATAGCATTCCTTTGTGGGAAATTCACATTTTCAACTACCCTACAAGTCGTGCTGATTGTTGTGTCATTTTCAAAGTTCACCATTCTGTAGCCGATGGCTATTGTCTCATGAACACTCTTTTGAGTTGTCTTAAGCGAGCCGATGATCCTTCTCTTCCTCTCACTTTTCCATCTCGAAAACGCTCTCAACAACCCAACGAAGTCCCCAAACGTGGTCGTTTGCCTCGTGTTTTCTCTTATGTTTGCGACTTTGTATCGAACTTTCGATGGAGTATCATGAAGAACACTTTTGTTGAAGACGAACCAACACCCATCAAACCGGAAGGAGAATCCATGCAACTTGTTAAGCCTATTGCCATATCCACCATGACATTTTCCCTTGATCAAATCAAACAGATTAAGAATAAACTTAATGCGGTACGATACTTCATAACTTTTTTTTGCTTTCATATCCATGAGTATTCTATACTAATTTATGCCCATTGTTGTATTGTCTATCCTATTATATTTGATTGTTAAAGAAATTAAAAAAAAAAAAAAAAAATTCATTCTTAGTTGAGGTAGATGACTATAAAGAATTGTACAAATTTTCTCCAAGTACTTATGATTCACGAGACCTTTATTTAATTAGTAGGTCAATCCACAATGATAACATTAATTTTTCCTTAAAACTCATGATATATTTTCCATAAACCTTGGATCAAAGCTTAAAGTATTGAGATTTGAGAATTTGATGTCATTACTTGCATCATATAAATATCAATTAAATATACAAAAATAAAAAATAAACACTCATTCAAATCAACATAAATATTAAACAACCTTCAATTTGAATTACAAATATTTTATGTTTTACAACTTTGGAGATTATGTCTATATAGTTTTATCATAAAACAGTTTTTACAGTCACTGCACATGTTGGTTCGATATTTTTAATGTTAACATTGTTGTTTTCATGACTATGTAACTATTTATACTTTTTTTTTAAAACTTGGTAATTATTTATTAACTAGATTAATCTAGTTGACATAATGAAATCAGGTAAATTATAAAGTGTTTAGAGGCGGTAATAAATTTAACTCACGATAATTATTTTGTTATGAATTATCTTTCATAACACAATATAGTAGTATCAGCTACAACCTAACTGCAAGTGCACGCAAATGGCTCGAACACTCATGAATATTTCACAAAAATAATTCATACACATGTTCGAGTAACGGTTTTATGTGACATTGATTATTAACAGAGTGTGAACGATGTGCTAACCGGAATCATATTCTTGGGAATTCGACTGTACATGCAAGAACATAGCCCTGAATCAAGCAAAGCAAATTCTAGTGCATTGATTCTTCTCAATACTCGAAAGGCCAAATCTTACAAATCAGTTAAAGAAATGCTCAAAGCTGATACAGATGCACCATGGGGAAATAAAATTGCTTTTTTGCCAGTCCCAATTCCCAAACTTATTGATTCTACAATTTCACCTTTGGAATTTGTGAAGGAAGCCAAAGCTAAAATTACTTTGCAAAAAAGCCCTCTCTCTGTTTTCATGGCTGCTAAAGTTTTTGAAATGCTAAATTTTGTGACAGGCCCTGAGGTGAATAAAATTTAACTTTTTCCCCTTCCCCATTTTTTTTTTTCTTACCTTTTGTCTTGTGTGTTATAGGTTGGTGCAAAGCTGTTCAAAAGGAAGCTGAAGAATTCAAGCATTATGATATCAAATATGATTGGTCCTGTGGAGAAGATGGCTTTGGCCAATCATCCAGTGAAAGGCCTATATTTTACTGTTCCTGGCATGCCTCAGGTATATTATCTAATCATATATAATTCTCCTAACAACTAAATTTGATTTAAGAGTATGTTTTGAGTTTCGGGTTTTTGTGAGTTTGGCTAATTAATACACAATTGTTCACCTAATAAAGATACGATATTGCCTATTTTAAATTTAAGAACTTGGTAGCTTTACTCTTTTTGGATAAAGTCTCGTAGATATAATTTGTCCTTCTTACAAAACGATCATGTCTTGAATGAGTCTTAAGCTTAAGTTTGATATTTAAATTATTAAATTATTTATTCTCACATATATTTAACATGTGTCTATCTTAAATTGTAGAGTCTAATGATAACAATTGTGAGTTACATGGGAGACTTGAGGATTGTGTTTGGCGGTGAGAAGTGCTTCATAGATCAACAAAAGTTGAAGAGATGCATTGAAGATGCCTTCAACAAGACTTTCATCAATGTTATTAACTCTTAATTAATATCTTTTTTAGGTTTGTGGTGTGTAAAAGCTTTTTAATTTTATGTGTTTCATTCCTACCATGGAGAATTTAAGTTTTTAATGCTTGTGAGTGTCAAC

mRNA sequence

CTACCACATAAGCTAAAGCAACTTTGGATTATTGGCAGCGAAAGATATATTTGGACAAAATAATGGAGATTTTGGAAGATTCAGCAACAGCGACCCCCATGAGCCCTTTCAGCCAATATTTCAACACTAGTGAAATTTGTGTGTCTGTTTTGGGAGTAATCGAACTCGAATCTCCAATTACTCACTCCGAAGCTTTGTCAATTGTCACTGATGTTCTCATCCCTGCCAATCCACGTTTCACTTCGATCATGGTGAAAGAAAAGTGTTGGGAAGAAAGAAAATGGAAGGCAACTAAGGTTAACCTAGAAGACCACATATACACACCAAAATTTCCCATGAATCTCTCCCTTTATGAATATGATGCTTACTTTGATGAATATGCAACCAAAACAGCTACAAAACCCTTTTCACATAGCATTCCTTTGTGGGAAATTCACATTTTCAACTACCCTACAAGTCGTGCTGATTGTTGTGTCATTTTCAAAGTTCACCATTCTGTAGCCGATGGCTATTGTCTCATGAACACTCTTTTGAGTTGTCTTAAGCGAGCCGATGATCCTTCTCTTCCTCTCACTTTTCCATCTCGAAAACGCTCTCAACAACCCAACGAAGTCCCCAAACGTGGTCGTTTGCCTCGTGTTTTCTCTTATGTTTGCGACTTTGTATCGAACTTTCGATGGAGTATCATGAAGAACACTTTTGTTGAAGACGAACCAACACCCATCAAACCGGAAGGAGAATCCATGCAACTTGTTAAGCCTATTGCCATATCCACCATGACATTTTCCCTTGATCAAATCAAACAGATTAAGAATAAACTTAATGCGAGTGTGAACGATGTGCTAACCGGAATCATATTCTTGGGAATTCGACTGTACATGCAAGAACATAGCCCTGAATCAAGCAAAGCAAATTCTAGTGCATTGATTCTTCTCAATACTCGAAAGGCCAAATCTTACAAATCAGTTAAAGAAATGCTCAAAGCTGATACAGATGCACCATGGGGAAATAAAATTGCTTTTTTGCCAGTCCCAATTCCCAAACTTATTGATTCTACAATTTCACCTTTGGAATTTGTGAAGGAAGCCAAAGCTAAAATTACTTTGCAAAAAAGCCCTCTCTCTGTTTTCATGGCTGCTAAAGTTTTTGAAATGCTAAATTTTGTGACAGGCCCTGAGGTGAATAAAATTTAACTTTTTCCCCTTCCCCATTTTTTTTTTTCTTACCTTTTGTCTTGTGTGTTATAGGTTGGTGCAAAGCTGTTCAAAAGGAAGCTGAAGAATTCAAGCATTATGATATCAAATATGATTGGTCCTGTGGAGAAGATGGCTTTGGCCAATCATCCAGTGAAAGGCCTATATTTTACTGTTCCTGGCATGCCTCAGAGTCTAATGATAACAATTGTGAGTTACATGGGAGACTTGAGGATTGTGTTTGGCGGTGAGAAGTGCTTCATAGATCAACAAAAGTTGAAGAGATGCATTGAAGATGCCTTCAACAAGACTTTCATCAATGTTATTAACTCTTAATTAATATCTTTTTTAGGTTTGTGGTGTGTAAAAGCTTTTTAATTTTATGTGTTTCATTCCTACCATGGAGAATTTAAGTTTTTAATGCTTGTGAGTGTCAAC

Coding sequence (CDS)

ATGGAGATTTTGGAAGATTCAGCAACAGCGACCCCCATGAGCCCTTTCAGCCAATATTTCAACACTAGTGAAATTTGTGTGTCTGTTTTGGGAGTAATCGAACTCGAATCTCCAATTACTCACTCCGAAGCTTTGTCAATTGTCACTGATGTTCTCATCCCTGCCAATCCACGTTTCACTTCGATCATGGTGAAAGAAAAGTGTTGGGAAGAAAGAAAATGGAAGGCAACTAAGGTTAACCTAGAAGACCACATATACACACCAAAATTTCCCATGAATCTCTCCCTTTATGAATATGATGCTTACTTTGATGAATATGCAACCAAAACAGCTACAAAACCCTTTTCACATAGCATTCCTTTGTGGGAAATTCACATTTTCAACTACCCTACAAGTCGTGCTGATTGTTGTGTCATTTTCAAAGTTCACCATTCTGTAGCCGATGGCTATTGTCTCATGAACACTCTTTTGAGTTGTCTTAAGCGAGCCGATGATCCTTCTCTTCCTCTCACTTTTCCATCTCGAAAACGCTCTCAACAACCCAACGAAGTCCCCAAACGTGGTCGTTTGCCTCGTGTTTTCTCTTATGTTTGCGACTTTGTATCGAACTTTCGATGGAGTATCATGAAGAACACTTTTGTTGAAGACGAACCAACACCCATCAAACCGGAAGGAGAATCCATGCAACTTGTTAAGCCTATTGCCATATCCACCATGACATTTTCCCTTGATCAAATCAAACAGATTAAGAATAAACTTAATGCGAGTGTGAACGATGTGCTAACCGGAATCATATTCTTGGGAATTCGACTGTACATGCAAGAACATAGCCCTGAATCAAGCAAAGCAAATTCTAGTGCATTGATTCTTCTCAATACTCGAAAGGCCAAATCTTACAAATCAGTTAAAGAAATGCTCAAAGCTGATACAGATGCACCATGGGGAAATAAAATTGCTTTTTTGCCAGTCCCAATTCCCAAACTTATTGATTCTACAATTTCACCTTTGGAATTTGTGAAGGAAGCCAAAGCTAAAATTACTTTGCAAAAAAGCCCTCTCTCTGTTTTCATGGCTGCTAAAGTTTTTGAAATGCTAAATTTTGTGACAGGCCCTGAGGTGAATAAAATTTAA

Protein sequence

MEILEDSATATPMSPFSQYFNTSEICVSVLGVIELESPITHSEALSIVTDVLIPANPRFTSIMVKEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSIPLWEIHIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQPNEVPKRGRLPRVFSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPIAISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHSPESSKANSSALILLNTRKAKSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTISPLEFVKEAKAKITLQKSPLSVFMAAKVFEMLNFVTGPEVNKI
Homology
BLAST of Sed0002925.2 vs. NCBI nr
Match: XP_022147882.1 (O-acyltransferase WSD1-like [Momordica charantia] >XP_022147883.1 O-acyltransferase WSD1-like [Momordica charantia])

HSP 1 Score: 586.6 bits (1511), Expect = 1.5e-163
Identity = 293/375 (78.13%), Postives = 327/375 (87.20%), Query Frame = 0

Query: 1   MEILEDSATATPMSPFSQYFNTSEICVSVLGVIELESPITHSEALSIVTDVLIPANPRFT 60
           MEILEDS   TPMSP SQYFNTSE+CVSVLGV+ELESPI+  +A+SIVTDVL+PANPRFT
Sbjct: 1   MEILEDS--TTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFT 60

Query: 61  SIMVKEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSIP 120
           SIMVKEK   ERKWK  +VN  DHIY P FP +LS YEYDAYFDEYATKTATKPFS SIP
Sbjct: 61  SIMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIP 120

Query: 121 LWEIHIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQ 180
           LWEIH+FNYPTS A C +IFKVHHS+ADG+CLMNTLLSCLKRADDPSLPLTFPSRK S+Q
Sbjct: 121 LWEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQ 180

Query: 181 PNEVPKRGRLPRVFSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPIAISTMT 240
           P    + G L  +   +   VSNF WS+MKNTFVED+PTPIKPEG+ MQLVKPIAISTMT
Sbjct: 181 PKNDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMT 240

Query: 241 FSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHSPESSKANSSALILLNTRKAKSYK 300
           FSLDQIKQIKN LN S+NDVLTGIIF+G+RLYMQEH+P+SS+ NSSALILLNTRKAK+YK
Sbjct: 241 FSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYK 300

Query: 301 SVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTIS--PLEFVKEAKAKITLQKSPLSVFMA 360
           SVKEML+ DTDAPWGN+IAFLPVPIPKLIDST+S  PLEFVKEAK KI LQ++ LSVFMA
Sbjct: 301 SVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMA 360

Query: 361 AKVFEMLNFVTGPEV 374
           AKVF+++N VTGPEV
Sbjct: 361 AKVFDIVNHVTGPEV 373

BLAST of Sed0002925.2 vs. NCBI nr
Match: XP_011659709.2 (O-acyltransferase WSD1 [Cucumis sativus] >KGN43701.2 hypothetical protein Csa_017048 [Cucumis sativus])

HSP 1 Score: 582.4 bits (1500), Expect = 2.8e-162
Identity = 299/381 (78.48%), Postives = 331/381 (86.88%), Query Frame = 0

Query: 1   MEILEDSATATPMSPFSQYFNTSEICVSVLGVIELESPITHSEAL-SIVTDVLIPANPRF 60
           MEI EDS    PMSP SQYFNT E+CVSVLGVIELE+PI+  E + SIVTDVL+PANPRF
Sbjct: 1   MEIYEDS--TAPMSPLSQYFNTCEMCVSVLGVIELETPISSWEDISSIVTDVLVPANPRF 60

Query: 61  TSIMVKEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSI 120
           TSIMVKEK  EERKWK TKVN +DHIY P FPM LS YEYDAYFDEYATKTATKPFS +I
Sbjct: 61  TSIMVKEKT-EERKWKTTKVNPKDHIYIPNFPMELSSYEYDAYFDEYATKTATKPFSQAI 120

Query: 121 PLWEIHIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQ 180
           PLWEIH+FNYPT+ A C +IFKVHHS+ADG+CLMNTLLSCLKRADDPSLPLTFPSR+RS+
Sbjct: 121 PLWEIHVFNYPTTHATCSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSK 180

Query: 181 QPNEVPKRGRL----PRVFSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPIA 240
           QP   PK  RL     R FS + +FV NF WSIMKNTFVED+PTPIKP+ +SMQLVKPIA
Sbjct: 181 QPGNEPKFFRLSHFPARFFSSISNFVLNFGWSIMKNTFVEDDPTPIKPQKDSMQLVKPIA 240

Query: 241 ISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHSPESSKANSSALILLNTRK 300
           ISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEH+PESS ANSSALILLNTRK
Sbjct: 241 ISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHNPESSGANSSALILLNTRK 300

Query: 301 AKSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTI---SPLEFVKEAKAKITLQKSP 360
           AK+YKSVKEM+K D+DAPWGNKIAFLP+PIPKLIDS +   +PLEFV++ K KI LQ+SP
Sbjct: 301 AKAYKSVKEMVKKDSDAPWGNKIAFLPIPIPKLIDSPVVSSTPLEFVEKVKEKIMLQRSP 360

Query: 361 LSVFMAAKVFEMLNFVTGPEV 374
           LSVF+AAKVFE+L  VTGPE+
Sbjct: 361 LSVFLAAKVFEILKNVTGPEI 378

BLAST of Sed0002925.2 vs. NCBI nr
Match: XP_022952997.1 (O-acyltransferase WSD1-like [Cucurbita moschata])

HSP 1 Score: 563.5 bits (1451), Expect = 1.3e-156
Identity = 291/379 (76.78%), Postives = 327/379 (86.28%), Query Frame = 0

Query: 1   MEILEDSATATPMSPFSQYFNTSEICVSVLGVIELESPITHSEALSIVTDVLIPANPRFT 60
           MEILEDS  A PMSP SQYFNTS +CVSVLG IELE+PI+ S A++I T VLIPANPRF+
Sbjct: 1   MEILEDS--AAPMSPLSQYFNTSAMCVSVLGFIELEAPISASHAMNIGTHVLIPANPRFS 60

Query: 61  SIMVKEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSIP 120
           SIMVKEK + ERKWK  +VNLEDHIYTPKFP++LS YEYDAYFDEYAT TATKPFS SIP
Sbjct: 61  SIMVKEKWFGERKWKMVEVNLEDHIYTPKFPVDLSPYEYDAYFDEYATNTATKPFSQSIP 120

Query: 121 LWEIHIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQ 180
           LWEIH+FNYPT+ A C +IFKVHHS+ADG+CLMNTLLS LKRADDPSLPLTFPSRKRS+Q
Sbjct: 121 LWEIHVFNYPTTHAPCTLIFKVHHSIADGFCLMNTLLSFLKRADDPSLPLTFPSRKRSKQ 180

Query: 181 PNEVPKRGRLPR----VFSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPIAI 240
           P      GRL      +F  + +F+SNF +SI  NTFVED+PTPIK EG+SMQLVKPIAI
Sbjct: 181 PENAHDFGRLSHFPACLFLSLRNFLSNFGYSI-ANTFVEDDPTPIKHEGDSMQLVKPIAI 240

Query: 241 STMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHSPESSKANSSALILLNTRKA 300
           STMTFSLDQIKQIK KLNA VNDV+TGIIFLGIRLYMQEHSP+S +ANSSALILLNTRKA
Sbjct: 241 STMTFSLDQIKQIKTKLNARVNDVITGIIFLGIRLYMQEHSPDSWEANSSALILLNTRKA 300

Query: 301 KSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTIS--PLEFVKEAKAKITLQKSPLS 360
           K+YKSV+EM+K D+D PWGN+IAFLPVPIPKL+DST+S  PLEFVKEAK  I LQ+SPLS
Sbjct: 301 KAYKSVQEMVKTDSDVPWGNRIAFLPVPIPKLMDSTLSTAPLEFVKEAKKNIMLQRSPLS 360

Query: 361 VFMAAKVFEMLNFVTGPEV 374
           VFMAAKVF+ML  VTGPE+
Sbjct: 361 VFMAAKVFDMLKHVTGPEM 376

BLAST of Sed0002925.2 vs. NCBI nr
Match: XP_038887587.1 (O-acyltransferase WSD1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 555.1 bits (1429), Expect = 4.8e-154
Identity = 288/381 (75.59%), Postives = 324/381 (85.04%), Query Frame = 0

Query: 1   MEILEDSATATPMSPFSQYFNTSEICVSVLGVIELESPITHSEAL-SIVTDVLIPANPRF 60
           ME  E+S    PMSP SQYFNTSE+CVSVLGVIELE+PI+  E + SI  DVLIP+NPRF
Sbjct: 1   MENYEES--TAPMSPMSQYFNTSEMCVSVLGVIELETPISAWEEISSIAIDVLIPSNPRF 60

Query: 61  TSIMVKEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSI 120
           TS+MVKE    ERKWK  K+N +DHIY P FP  LS YEYDAYFDEYAT+TATKPFS SI
Sbjct: 61  TSVMVKESSNGERKWKTIKINPKDHIYIPNFPPELSPYEYDAYFDEYATQTATKPFSQSI 120

Query: 121 PLWEIHIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQ 180
           PLWEIH+FNYPTS A C +IFKVHHS+ADG+CLMN LLSCLKRADDPSLPLTFPSR+RS+
Sbjct: 121 PLWEIHVFNYPTSHATCSIIFKVHHSIADGFCLMNILLSCLKRADDPSLPLTFPSRQRSK 180

Query: 181 QP-NEVPKRGRL---PRVFSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPIA 240
           QP NE+  R R      +FS V +F+ NF WSIMKNT VED+PTPIKPE +SMQLVKPIA
Sbjct: 181 QPENELNFRRRSYFPGHLFSSVSNFLLNFGWSIMKNTIVEDDPTPIKPERDSMQLVKPIA 240

Query: 241 ISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHSPESSKANSSALILLNTRK 300
           IST+TF LDQIKQIK+KLNASVNDV+TGIIFLGIRLYMQEH+PESS+ANSSALILLNTRK
Sbjct: 241 ISTITFPLDQIKQIKDKLNASVNDVVTGIIFLGIRLYMQEHNPESSQANSSALILLNTRK 300

Query: 301 AKSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTI---SPLEFVKEAKAKITLQKSP 360
           AK+YKSVKEM+K +TDAPWGNKIAFLPVPIPKLIDST    +PLEFVK+AK KI LQ+SP
Sbjct: 301 AKAYKSVKEMVKTNTDAPWGNKIAFLPVPIPKLIDSTTLASTPLEFVKKAKEKIMLQRSP 360

Query: 361 LSVFMAAKVFEMLNFVTGPEV 374
           +SVF+AAK FEM+  VTGPEV
Sbjct: 361 VSVFLAAKFFEMVKNVTGPEV 379

BLAST of Sed0002925.2 vs. NCBI nr
Match: XP_038887586.1 (O-acyltransferase WSD1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 555.1 bits (1429), Expect = 4.8e-154
Identity = 288/381 (75.59%), Postives = 324/381 (85.04%), Query Frame = 0

Query: 1   MEILEDSATATPMSPFSQYFNTSEICVSVLGVIELESPITHSEAL-SIVTDVLIPANPRF 60
           ME  E+S    PMSP SQYFNTSE+CVSVLGVIELE+PI+  E + SI  DVLIP+NPRF
Sbjct: 1   MENYEES--TAPMSPMSQYFNTSEMCVSVLGVIELETPISAWEEISSIAIDVLIPSNPRF 60

Query: 61  TSIMVKEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSI 120
           TS+MVKE    ERKWK  K+N +DHIY P FP  LS YEYDAYFDEYAT+TATKPFS SI
Sbjct: 61  TSVMVKESSNGERKWKTIKINPKDHIYIPNFPPELSPYEYDAYFDEYATQTATKPFSQSI 120

Query: 121 PLWEIHIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQ 180
           PLWEIH+FNYPTS A C +IFKVHHS+ADG+CLMN LLSCLKRADDPSLPLTFPSR+RS+
Sbjct: 121 PLWEIHVFNYPTSHATCSIIFKVHHSIADGFCLMNILLSCLKRADDPSLPLTFPSRQRSK 180

Query: 181 QP-NEVPKRGRL---PRVFSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPIA 240
           QP NE+  R R      +FS V +F+ NF WSIMKNT VED+PTPIKPE +SMQLVKPIA
Sbjct: 181 QPENELNFRRRSYFPGHLFSSVSNFLLNFGWSIMKNTIVEDDPTPIKPERDSMQLVKPIA 240

Query: 241 ISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHSPESSKANSSALILLNTRK 300
           IST+TF LDQIKQIK+KLNASVNDV+TGIIFLGIRLYMQEH+PESS+ANSSALILLNTRK
Sbjct: 241 ISTITFPLDQIKQIKDKLNASVNDVVTGIIFLGIRLYMQEHNPESSQANSSALILLNTRK 300

Query: 301 AKSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTI---SPLEFVKEAKAKITLQKSP 360
           AK+YKSVKEM+K +TDAPWGNKIAFLPVPIPKLIDST    +PLEFVK+AK KI LQ+SP
Sbjct: 301 AKAYKSVKEMVKTNTDAPWGNKIAFLPVPIPKLIDSTTLASTPLEFVKKAKEKIMLQRSP 360

Query: 361 LSVFMAAKVFEMLNFVTGPEV 374
           +SVF+AAK FEM+  VTGPEV
Sbjct: 361 VSVFLAAKFFEMVKNVTGPEV 379

BLAST of Sed0002925.2 vs. ExPASy Swiss-Prot
Match: Q9M3B2 (Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=WSD5 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 6.3e-32
Identity = 107/389 (27.51%), Postives = 182/389 (46.79%), Query Frame = 0

Query: 12  PMSPFSQYFNTSEICVSVLGVIELESPITHSEALSIVTDVLIPANPRFTSIMVK--EKCW 71
           P+SP ++ F+  E    ++ V+ L++ I     +  +   L+  +PRF+S +V       
Sbjct: 27  PLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLM-RHPRFSSKLVNNCNNNR 86

Query: 72  EERKWKATKVNLEDHIYTPKF-PMNLSLYEYDAYFDEYATKTATKPFSHSIPLWEIHIFN 131
           +E+KW  T V +EDH+  PK    ++     D + + Y +   T P   S PLWE+H+ +
Sbjct: 87  QEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKPLWEVHLLD 146

Query: 132 YPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQPNEVPKRG 191
             TS A+   + ++HHS+ DG  +M+ +L+C ++  +P+   + P + R       P  G
Sbjct: 147 LKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNR-------PSSG 206

Query: 192 RLPRVFSYVCDFVSNFRWSIM---------KNT------------FVEDEPTPIKPEGES 251
                 S  C   S F W +M          NT            F++D  TPIK + + 
Sbjct: 207 SSSLKTSSRC--YSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPIKGDFK- 266

Query: 252 MQLVKPIAISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEH--SPESSKANS 311
           +   K + +   T SLD IK IKN +  +VNDV+ G+   G+  Y++      E SK NS
Sbjct: 267 LSKGKRMCMVHRTVSLDDIKLIKNAMKMTVNDVVLGVSQAGLSQYLKRRYGEQEESKRNS 326

Query: 312 S---------ALILLNTRKAKSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTISPL 366
           S         A +L+N R     + + +M+   +   WGN I ++  P    I     PL
Sbjct: 327 SNIPKGIRLRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYIIFPFS--IALCDDPL 386

BLAST of Sed0002925.2 vs. ExPASy Swiss-Prot
Match: Q5KS41 (Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=WSD11 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 8.2e-32
Identity = 115/403 (28.54%), Postives = 194/403 (48.14%), Query Frame = 0

Query: 5   EDSATA------TPMSPFSQYFNTSE----ICVSVLGVIELESP-ITHSEALSIVTDVLI 64
           ED  TA       P+SP S+ FN+ +    I V++   ++ ++P I H    ++V     
Sbjct: 3   EDKKTARETVEEEPLSPCSRLFNSPDFNCAIIVTMGSKVKGDTPAIIHGLEHTLVN---- 62

Query: 65  PANPRFTSIMVKEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATK 124
             +PRF+SI+      ++ +W  TKV +E+H+  P   ++  +   D Y ++Y +K  T 
Sbjct: 63  --HPRFSSILEMNN-GKKPRWVRTKVKVEEHVIVP--DVDPDIENPDQYLEDYISKLTTI 122

Query: 125 PFSHSIPLWEIHIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFP 184
           P   S PLWE+H+    TS A+   I K+HHS+ DG  LM+ LL+C ++  DP    T  
Sbjct: 123 PMDLSKPLWEMHLLGVKTSNAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVA 182

Query: 185 SRKRSQQPNEVPKRGRLPRVFSYVCDFVSNFRWSIMK---NTFVE------------DEP 244
             K+   P+     G   +++     FV    W I++   NTFV+            D  
Sbjct: 183 VHKKRFGPS--CNSGFFNKIWWL---FVG--LWFILRLLFNTFVDILMFALTIFVLRDTE 242

Query: 245 TPI--KPEGESMQLVKPIAISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGI-----RL 304
           TP+  KP  E +    P        S D +K +KN +  +VNDVL G+   G+     R 
Sbjct: 243 TPLLAKPGSELI----PKRFVHRIISFDDVKLVKNAMKMTVNDVLLGVTQAGLSRYLSRK 302

Query: 305 YMQEHSPESSKA----NSSALILLNTRKAKSYKSVKEMLKADTDAPWGNKIAFLPVPIPK 364
           Y QE +P+S ++       + I++N R     +++ +M+   +   WGN   ++ +P   
Sbjct: 303 YDQEATPKSKESMRRIRLRSAIMINLRPNAGIEALADMMAKKSKCRWGNLFGYILLPFSV 362

Query: 365 LIDSTISPLEFVKEAKAKITLQKSPLSVFMAAKVFEMLNFVTG 371
            +++   PLE+V++AKA I  +K  L    +   F+++  V G
Sbjct: 363 GLET--DPLEYVRQAKATIDRKKHSLEAVFSMAFFKLILKVLG 383

BLAST of Sed0002925.2 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 4.1e-31
Identity = 99/368 (26.90%), Postives = 175/368 (47.55%), Query Frame = 0

Query: 12  PMSPFSQYFNTSEICVSVLGVIELESPITHSEALSIVTDVLIPANPRFTSIMVKEKCWEE 71
           P+SP ++ F+  E   +++ VI  +S +     +    +  I  +PRF+S +V ++  + 
Sbjct: 19  PLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKESFI-RHPRFSSKLVTDENGQN 78

Query: 72  RKWKATKVNLEDHIYTPKF-PMNLSLYEYDAYFDEYATKTATKPFSHSIPLWEIHIFNYP 131
           ++W  T V +EDH   PK  P N+     +A+ ++Y +     P   S PLWE+H+ +  
Sbjct: 79  QRWVRTNVVVEDHFIVPKIKPQNIE--NSNAFLEDYVSDLMKIPLDTSRPLWELHLLDLK 138

Query: 132 TSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQPNEVPKRGR- 191
           TS A+   + K+HHSV DG  +M+ +L+C+++  +P    + P + RS   + +   G  
Sbjct: 139 TSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLLTTGSR 198

Query: 192 -------LPRV--------FSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPI 251
                  L +V         + VCD +      I+   FV+D  TPIK +  S +  K +
Sbjct: 199 SDSRLLWLVKVIWTAVILGLNTVCDALE----FIVTTLFVKDTETPIKGDFLSTK-SKQL 258

Query: 252 AISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHSPESSKANSS-------- 311
            +   T SLD IK  KN +N ++NDV+ G+   G+  Y+     E    N          
Sbjct: 259 RLVHRTVSLDDIKLTKNAMNMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKLPKRI 318

Query: 312 ---ALILLNTRKAKSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTISPLEFVKEAK 352
              + +L+N R     + + +M++  +   WGN   +  V  P  I     PLE ++ A+
Sbjct: 319 RLRSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGY--VVFPFSIALRDDPLEHLEIAQ 376

BLAST of Sed0002925.2 vs. ExPASy Swiss-Prot
Match: F4IU14 (Wax ester synthase/diacylglycerol acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=WSD3 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 5.0e-29
Identity = 95/380 (25.00%), Postives = 172/380 (45.26%), Query Frame = 0

Query: 5   EDSATATPMSPFSQYFNTSEICVSVLGVIELESPITHSEALSIVTDVLIPANPRFTSIMV 64
           +++A   P+SP SQ F +  +   ++  +  ++    S  +  V +  I   PRF+S  V
Sbjct: 15  QETAAIEPLSPVSQLFVSPSLYCFIIFTLGFQTRCNPSTIVEGVKNTWIKL-PRFSS-KV 74

Query: 65  KEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSIPLWEI 124
           + K   +  W    V +EDH+  P    + ++   D + ++Y +K A  P   S PLWE+
Sbjct: 75  EIKKNGKASWVPVSVRVEDHVVVPDLDYS-NIENPDQFIEDYTSKLANTPMDMSRPLWEL 134

Query: 125 HIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDP-SLPLTFPSRKR-SQQPN 184
           H+ N  TS A+   I K HHS+ DG  L++ LL+  ++  DP +LP T  +RK  S    
Sbjct: 135 HLLNIKTSNAESLAIGKFHHSLGDGMSLISLLLASSRKTSDPDALPTTAATRKHASSNKK 194

Query: 185 EVPKRGRLPRVFSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPIAISTMTFS 244
                GR   +   +   V      ++   F+ D  TP+   G++   ++   +     S
Sbjct: 195 SWWLVGRFWFMIRIIFTTVVELFKYLLTLCFMRDTKTPLM--GKTGDAIRSRKVIHRIVS 254

Query: 245 LDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQ---------EHSPESSKANSSALILLNT 304
            D +K +KN ++  VNDVL G+   G+  Y+          E      K      + +N 
Sbjct: 255 FDDVKLVKNNMDMKVNDVLLGMTQAGLSRYLSRKYDEDMVVEKKKNLEKIRLRGTVFVNL 314

Query: 305 RKAKSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTISPLEFVKEAKAKITLQKSPL 364
           R     + +  M+   +   WGN +  +  P+   + S   PLE+V+ AK+ + ++K  +
Sbjct: 315 RADTKLEDLANMMAKGSKCRWGNFVGVIIFPL--WVRSEDDPLEYVRRAKSTMDIKKLSI 374

Query: 365 SVFMAAKVFEMLNFVTGPEV 374
              +   + ++   + G +V
Sbjct: 375 ESLICYGLIKLTRKILGGKV 387

BLAST of Sed0002925.2 vs. ExPASy Swiss-Prot
Match: Q9C7H4 (Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=WSD2 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.4e-28
Identity = 100/366 (27.32%), Postives = 177/366 (48.36%), Query Frame = 0

Query: 12  PMSPFSQYFNTSEICVSVLGVIELESPITHSEALSIVTDVLIPANPRFTSIMVKEKCWEE 71
           P+SPF++ F+   + V  +  I  ++    S  +  + + LI  +PRF+SI+V      +
Sbjct: 13  PVSPFARLFSLPGLDVFNIVTIGCKTEGNASTIVEGIKNTLI-NHPRFSSILVTGHGEHK 72

Query: 72  RK--WKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSIPLWEIHIFNY 131
            K  W  TK+N+E+H+  P    N+     D + ++Y +  A  P   S PLWE H+   
Sbjct: 73  GKARWIPTKINVEEHVIVPDIDPNIE--NPDEFLEDYTSNMALSPMDMSKPLWEFHLLKL 132

Query: 132 PTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQPNEVPKRGR 191
            TS A+   + + HHS+ DG  LM+ LL+C ++  DP    TF + K+++  N       
Sbjct: 133 KTSHAEAVTVARFHHSLGDGMSLMSLLLACTRKTCDPEAFPTFVAPKKNKAKN------- 192

Query: 192 LPRVFSYVCDFVSNFRWSIMK---NTFVEDEPTPI---KPEGESMQLV-KPIA-ISTMTF 251
                  VC  +  + W I++   +T VE   + +   +    S  ++ KP A +S   F
Sbjct: 193 -------VCFSLVAWLWFIVRLMFHTCVEVIKSIVFICRASDTSAHIMGKPGATLSANKF 252

Query: 252 -----SLDQIKQIKNKLNASVNDVLTGIIFLGIRLYM-QEHSPESSKANSSAL------- 311
                SLD +K +KN +N +VNDVL G++  G+  Y+ Q +  E+S  +   L       
Sbjct: 253 IHRIISLDDVKMVKNAMNMTVNDVLFGMVQAGLSRYLNQRYDLETSSKSRKNLHNIGLHG 312

Query: 312 -ILLNTRKAKSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTISPLEFVKEAKAKIT 354
            +  N R  ++ + + +M+   +   WGN I ++ +P+       +   E+V++AK  + 
Sbjct: 313 VVFFNLRPNRNIEDLAKMMAKGSKCRWGNSIGYVLIPLGMKPQDDV--FEYVRQAKTIMD 359

BLAST of Sed0002925.2 vs. ExPASy TrEMBL
Match: A0A6J1D2I7 (Diacylglycerol O-acyltransferase OS=Momordica charantia OX=3673 GN=LOC111016711 PE=4 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 7.1e-164
Identity = 293/375 (78.13%), Postives = 327/375 (87.20%), Query Frame = 0

Query: 1   MEILEDSATATPMSPFSQYFNTSEICVSVLGVIELESPITHSEALSIVTDVLIPANPRFT 60
           MEILEDS   TPMSP SQYFNTSE+CVSVLGV+ELESPI+  +A+SIVTDVL+PANPRFT
Sbjct: 1   MEILEDS--TTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFT 60

Query: 61  SIMVKEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSIP 120
           SIMVKEK   ERKWK  +VN  DHIY P FP +LS YEYDAYFDEYATKTATKPFS SIP
Sbjct: 61  SIMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIP 120

Query: 121 LWEIHIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQ 180
           LWEIH+FNYPTS A C +IFKVHHS+ADG+CLMNTLLSCLKRADDPSLPLTFPSRK S+Q
Sbjct: 121 LWEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQ 180

Query: 181 PNEVPKRGRLPRVFSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPIAISTMT 240
           P    + G L  +   +   VSNF WS+MKNTFVED+PTPIKPEG+ MQLVKPIAISTMT
Sbjct: 181 PKNDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMT 240

Query: 241 FSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHSPESSKANSSALILLNTRKAKSYK 300
           FSLDQIKQIKN LN S+NDVLTGIIF+G+RLYMQEH+P+SS+ NSSALILLNTRKAK+YK
Sbjct: 241 FSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYK 300

Query: 301 SVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTIS--PLEFVKEAKAKITLQKSPLSVFMA 360
           SVKEML+ DTDAPWGN+IAFLPVPIPKLIDST+S  PLEFVKEAK KI LQ++ LSVFMA
Sbjct: 301 SVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMA 360

Query: 361 AKVFEMLNFVTGPEV 374
           AKVF+++N VTGPEV
Sbjct: 361 AKVFDIVNHVTGPEV 373

BLAST of Sed0002925.2 vs. ExPASy TrEMBL
Match: A0A6J1GLZ3 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455512 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 6.5e-157
Identity = 291/379 (76.78%), Postives = 327/379 (86.28%), Query Frame = 0

Query: 1   MEILEDSATATPMSPFSQYFNTSEICVSVLGVIELESPITHSEALSIVTDVLIPANPRFT 60
           MEILEDS  A PMSP SQYFNTS +CVSVLG IELE+PI+ S A++I T VLIPANPRF+
Sbjct: 1   MEILEDS--AAPMSPLSQYFNTSAMCVSVLGFIELEAPISASHAMNIGTHVLIPANPRFS 60

Query: 61  SIMVKEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSIP 120
           SIMVKEK + ERKWK  +VNLEDHIYTPKFP++LS YEYDAYFDEYAT TATKPFS SIP
Sbjct: 61  SIMVKEKWFGERKWKMVEVNLEDHIYTPKFPVDLSPYEYDAYFDEYATNTATKPFSQSIP 120

Query: 121 LWEIHIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQ 180
           LWEIH+FNYPT+ A C +IFKVHHS+ADG+CLMNTLLS LKRADDPSLPLTFPSRKRS+Q
Sbjct: 121 LWEIHVFNYPTTHAPCTLIFKVHHSIADGFCLMNTLLSFLKRADDPSLPLTFPSRKRSKQ 180

Query: 181 PNEVPKRGRLPR----VFSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPIAI 240
           P      GRL      +F  + +F+SNF +SI  NTFVED+PTPIK EG+SMQLVKPIAI
Sbjct: 181 PENAHDFGRLSHFPACLFLSLRNFLSNFGYSI-ANTFVEDDPTPIKHEGDSMQLVKPIAI 240

Query: 241 STMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHSPESSKANSSALILLNTRKA 300
           STMTFSLDQIKQIK KLNA VNDV+TGIIFLGIRLYMQEHSP+S +ANSSALILLNTRKA
Sbjct: 241 STMTFSLDQIKQIKTKLNARVNDVITGIIFLGIRLYMQEHSPDSWEANSSALILLNTRKA 300

Query: 301 KSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTIS--PLEFVKEAKAKITLQKSPLS 360
           K+YKSV+EM+K D+D PWGN+IAFLPVPIPKL+DST+S  PLEFVKEAK  I LQ+SPLS
Sbjct: 301 KAYKSVQEMVKTDSDVPWGNRIAFLPVPIPKLMDSTLSTAPLEFVKEAKKNIMLQRSPLS 360

Query: 361 VFMAAKVFEMLNFVTGPEV 374
           VFMAAKVF+ML  VTGPE+
Sbjct: 361 VFMAAKVFDMLKHVTGPEM 376

BLAST of Sed0002925.2 vs. ExPASy TrEMBL
Match: A0A0A0K2E9 (Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051330 PE=4 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 6.7e-138
Identity = 251/319 (78.68%), Postives = 279/319 (87.46%), Query Frame = 0

Query: 62  IMVKEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSIPL 121
           + VKEK  EERKWK TKVN +DHIY P FPM LS YEYDAYFDEYATKTATKPFS +IPL
Sbjct: 1   MQVKEKT-EERKWKTTKVNPKDHIYIPNFPMELSSYEYDAYFDEYATKTATKPFSQAIPL 60

Query: 122 WEIHIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQP 181
           WEIH+FNYPT+ A C +IFKVHHS+ADG+CLMNTLLSCLKRADDPSLPLTFPSR+RS+QP
Sbjct: 61  WEIHVFNYPTTHATCSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 120

Query: 182 NEVPKRGRL----PRVFSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPIAIS 241
              PK  RL     R FS + +FV NF WSIMKNTFVED+PTPIKP+ +SMQLVKPIAIS
Sbjct: 121 GNEPKFFRLSHFPARFFSSISNFVLNFGWSIMKNTFVEDDPTPIKPQKDSMQLVKPIAIS 180

Query: 242 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHSPESSKANSSALILLNTRKAK 301
           TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEH+PESS ANSSALILLNTRKAK
Sbjct: 181 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHNPESSGANSSALILLNTRKAK 240

Query: 302 SYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTI---SPLEFVKEAKAKITLQKSPLS 361
           +YKSVKEM+K D+DAPWGNKIAFLP+PIPKLIDS +   +PLEFV++ K KI LQ+SPLS
Sbjct: 241 AYKSVKEMVKKDSDAPWGNKIAFLPIPIPKLIDSPVVSSTPLEFVEKVKEKIMLQRSPLS 300

Query: 362 VFMAAKVFEMLNFVTGPEV 374
           VF+AAKVFE+L  VTGPE+
Sbjct: 301 VFLAAKVFEILKNVTGPEI 318

BLAST of Sed0002925.2 vs. ExPASy TrEMBL
Match: A0A5D3C6U2 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002990 PE=4 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 8.2e-136
Identity = 250/319 (78.37%), Postives = 278/319 (87.15%), Query Frame = 0

Query: 62  IMVKEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSIPL 121
           + VKEK  EERKWK TKV+L+DHI  P FP  LS YEYDAYFDEYATKTATKPFS +IPL
Sbjct: 1   MQVKEKT-EERKWKTTKVSLKDHICIPNFPTELSSYEYDAYFDEYATKTATKPFSQAIPL 60

Query: 122 WEIHIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQP 181
           WEIH+FNYPT+ A C VIFKVHHS+ADG+CLMNTLLSCLKRADDPSLPLTFPSR+RS+QP
Sbjct: 61  WEIHVFNYPTTHATCSVIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 120

Query: 182 NEVPKRGRL----PRVFSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPIAIS 241
                  RL     R FS V +FV NF WSIMKNTFVED+PTPIKP+ +SMQLVKPIAIS
Sbjct: 121 KNKLDFCRLSHFPARFFSSVSNFVLNFGWSIMKNTFVEDDPTPIKPQEDSMQLVKPIAIS 180

Query: 242 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHSPESSKANSSALILLNTRKAK 301
           TMTFSLDQIKQIKNKLNASVNDVLTGIIFLG+RLYMQEH+PESS+ANSSALILLNTRKAK
Sbjct: 181 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGVRLYMQEHNPESSEANSSALILLNTRKAK 240

Query: 302 SYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTI---SPLEFVKEAKAKITLQKSPLS 361
           +YKSVKEM+K D+DAPWGNKIAFLPVPIPKLIDS +   +PLEFV++ K KITLQ+SPLS
Sbjct: 241 AYKSVKEMVKKDSDAPWGNKIAFLPVPIPKLIDSPVVSSTPLEFVEKVKEKITLQRSPLS 300

Query: 362 VFMAAKVFEMLNFVTGPEV 374
           VF+AAKVFE+L  VTGPE+
Sbjct: 301 VFLAAKVFEILKNVTGPEI 318

BLAST of Sed0002925.2 vs. ExPASy TrEMBL
Match: A0A5A7SPR9 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G002950 PE=4 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.8e-135
Identity = 249/319 (78.06%), Postives = 278/319 (87.15%), Query Frame = 0

Query: 62  IMVKEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSIPL 121
           + VKEK  EERKWK TKV+L+DHI  P FP  LS YEYDAYFDEY+TKTATKPFS +IPL
Sbjct: 1   MQVKEKT-EERKWKTTKVSLKDHICIPNFPTELSSYEYDAYFDEYSTKTATKPFSQAIPL 60

Query: 122 WEIHIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQP 181
           WEIH+FNYPT+ A C VIFKVHHS+ADG+CLMNTLLSCLKRADDPSLPLTFPSR+RS+QP
Sbjct: 61  WEIHVFNYPTTHATCSVIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 120

Query: 182 NEVPKRGRL----PRVFSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPIAIS 241
                  RL     R FS V +FV NF WSIMKNTFVED+PTPIKP+ +SMQLVKPIAIS
Sbjct: 121 KNKLDFCRLSHFPARFFSSVSNFVLNFGWSIMKNTFVEDDPTPIKPQEDSMQLVKPIAIS 180

Query: 242 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHSPESSKANSSALILLNTRKAK 301
           TMTFSLDQIKQIKNKLNASVNDVLTGIIFLG+RLYMQEH+PESS+ANSSALILLNTRKAK
Sbjct: 181 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGVRLYMQEHNPESSEANSSALILLNTRKAK 240

Query: 302 SYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTI---SPLEFVKEAKAKITLQKSPLS 361
           +YKSVKEM+K D+DAPWGNKIAFLPVPIPKLIDS +   +PLEFV++ K KITLQ+SPLS
Sbjct: 241 AYKSVKEMVKKDSDAPWGNKIAFLPVPIPKLIDSPVVSSTPLEFVEKVKEKITLQRSPLS 300

Query: 362 VFMAAKVFEMLNFVTGPEV 374
           VF+AAKVFE+L  VTGPE+
Sbjct: 301 VFLAAKVFEILKNVTGPEI 318

BLAST of Sed0002925.2 vs. TAIR 10
Match: AT3G49200.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 139.8 bits (351), Expect = 4.4e-33
Identity = 107/389 (27.51%), Postives = 182/389 (46.79%), Query Frame = 0

Query: 12  PMSPFSQYFNTSEICVSVLGVIELESPITHSEALSIVTDVLIPANPRFTSIMVK--EKCW 71
           P+SP ++ F+  E    ++ V+ L++ I     +  +   L+  +PRF+S +V       
Sbjct: 27  PLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLM-RHPRFSSKLVNNCNNNR 86

Query: 72  EERKWKATKVNLEDHIYTPKF-PMNLSLYEYDAYFDEYATKTATKPFSHSIPLWEIHIFN 131
           +E+KW  T V +EDH+  PK    ++     D + + Y +   T P   S PLWE+H+ +
Sbjct: 87  QEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKPLWEVHLLD 146

Query: 132 YPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQPNEVPKRG 191
             TS A+   + ++HHS+ DG  +M+ +L+C ++  +P+   + P + R       P  G
Sbjct: 147 LKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNR-------PSSG 206

Query: 192 RLPRVFSYVCDFVSNFRWSIM---------KNT------------FVEDEPTPIKPEGES 251
                 S  C   S F W +M          NT            F++D  TPIK + + 
Sbjct: 207 SSSLKTSSRC--YSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPIKGDFK- 266

Query: 252 MQLVKPIAISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEH--SPESSKANS 311
           +   K + +   T SLD IK IKN +  +VNDV+ G+   G+  Y++      E SK NS
Sbjct: 267 LSKGKRMCMVHRTVSLDDIKLIKNAMKMTVNDVVLGVSQAGLSQYLKRRYGEQEESKRNS 326

Query: 312 S---------ALILLNTRKAKSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTISPL 366
           S         A +L+N R     + + +M+   +   WGN I ++  P    I     PL
Sbjct: 327 SNIPKGIRLRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYIIFPFS--IALCDDPL 386

BLAST of Sed0002925.2 vs. TAIR 10
Match: AT5G53390.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 139.4 bits (350), Expect = 5.8e-33
Identity = 115/403 (28.54%), Postives = 194/403 (48.14%), Query Frame = 0

Query: 5   EDSATA------TPMSPFSQYFNTSE----ICVSVLGVIELESP-ITHSEALSIVTDVLI 64
           ED  TA       P+SP S+ FN+ +    I V++   ++ ++P I H    ++V     
Sbjct: 3   EDKKTARETVEEEPLSPCSRLFNSPDFNCAIIVTMGSKVKGDTPAIIHGLEHTLVN---- 62

Query: 65  PANPRFTSIMVKEKCWEERKWKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATK 124
             +PRF+SI+      ++ +W  TKV +E+H+  P   ++  +   D Y ++Y +K  T 
Sbjct: 63  --HPRFSSILEMNN-GKKPRWVRTKVKVEEHVIVP--DVDPDIENPDQYLEDYISKLTTI 122

Query: 125 PFSHSIPLWEIHIFNYPTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFP 184
           P   S PLWE+H+    TS A+   I K+HHS+ DG  LM+ LL+C ++  DP    T  
Sbjct: 123 PMDLSKPLWEMHLLGVKTSNAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVA 182

Query: 185 SRKRSQQPNEVPKRGRLPRVFSYVCDFVSNFRWSIMK---NTFVE------------DEP 244
             K+   P+     G   +++     FV    W I++   NTFV+            D  
Sbjct: 183 VHKKRFGPS--CNSGFFNKIWWL---FVG--LWFILRLLFNTFVDILMFALTIFVLRDTE 242

Query: 245 TPI--KPEGESMQLVKPIAISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGI-----RL 304
           TP+  KP  E +    P        S D +K +KN +  +VNDVL G+   G+     R 
Sbjct: 243 TPLLAKPGSELI----PKRFVHRIISFDDVKLVKNAMKMTVNDVLLGVTQAGLSRYLSRK 302

Query: 305 YMQEHSPESSKA----NSSALILLNTRKAKSYKSVKEMLKADTDAPWGNKIAFLPVPIPK 364
           Y QE +P+S ++       + I++N R     +++ +M+   +   WGN   ++ +P   
Sbjct: 303 YDQEATPKSKESMRRIRLRSAIMINLRPNAGIEALADMMAKKSKCRWGNLFGYILLPFSV 362

Query: 365 LIDSTISPLEFVKEAKAKITLQKSPLSVFMAAKVFEMLNFVTG 371
            +++   PLE+V++AKA I  +K  L    +   F+++  V G
Sbjct: 363 GLET--DPLEYVRQAKATIDRKKHSLEAVFSMAFFKLILKVLG 383

BLAST of Sed0002925.2 vs. TAIR 10
Match: AT3G49190.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 137.1 bits (344), Expect = 2.9e-32
Identity = 99/368 (26.90%), Postives = 175/368 (47.55%), Query Frame = 0

Query: 12  PMSPFSQYFNTSEICVSVLGVIELESPITHSEALSIVTDVLIPANPRFTSIMVKEKCWEE 71
           P+SP ++ F+  E   +++ VI  +S +     +    +  I  +PRF+S +V ++  + 
Sbjct: 19  PLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKESFI-RHPRFSSKLVTDENGQN 78

Query: 72  RKWKATKVNLEDHIYTPKF-PMNLSLYEYDAYFDEYATKTATKPFSHSIPLWEIHIFNYP 131
           ++W  T V +EDH   PK  P N+     +A+ ++Y +     P   S PLWE+H+ +  
Sbjct: 79  QRWVRTNVVVEDHFIVPKIKPQNIE--NSNAFLEDYVSDLMKIPLDTSRPLWELHLLDLK 138

Query: 132 TSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQPNEVPKRGR- 191
           TS A+   + K+HHSV DG  +M+ +L+C+++  +P    + P + RS   + +   G  
Sbjct: 139 TSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLLTTGSR 198

Query: 192 -------LPRV--------FSYVCDFVSNFRWSIMKNTFVEDEPTPIKPEGESMQLVKPI 251
                  L +V         + VCD +      I+   FV+D  TPIK +  S +  K +
Sbjct: 199 SDSRLLWLVKVIWTAVILGLNTVCDALE----FIVTTLFVKDTETPIKGDFLSTK-SKQL 258

Query: 252 AISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHSPESSKANSS-------- 311
            +   T SLD IK  KN +N ++NDV+ G+   G+  Y+     E    N          
Sbjct: 259 RLVHRTVSLDDIKLTKNAMNMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKLPKRI 318

Query: 312 ---ALILLNTRKAKSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTISPLEFVKEAK 352
              + +L+N R     + + +M++  +   WGN   +  V  P  I     PLE ++ A+
Sbjct: 319 RLRSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGY--VVFPFSIALRDDPLEHLEIAQ 376

BLAST of Sed0002925.2 vs. TAIR 10
Match: AT1G72110.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 128.6 bits (322), Expect = 1.0e-29
Identity = 100/366 (27.32%), Postives = 177/366 (48.36%), Query Frame = 0

Query: 12  PMSPFSQYFNTSEICVSVLGVIELESPITHSEALSIVTDVLIPANPRFTSIMVKEKCWEE 71
           P+SPF++ F+   + V  +  I  ++    S  +  + + LI  +PRF+SI+V      +
Sbjct: 13  PVSPFARLFSLPGLDVFNIVTIGCKTEGNASTIVEGIKNTLI-NHPRFSSILVTGHGEHK 72

Query: 72  RK--WKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSIPLWEIHIFNY 131
            K  W  TK+N+E+H+  P    N+     D + ++Y +  A  P   S PLWE H+   
Sbjct: 73  GKARWIPTKINVEEHVIVPDIDPNIE--NPDEFLEDYTSNMALSPMDMSKPLWEFHLLKL 132

Query: 132 PTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQPNEVPKRGR 191
            TS A+   + + HHS+ DG  LM+ LL+C ++  DP    TF + K+++  N       
Sbjct: 133 KTSHAEAVTVARFHHSLGDGMSLMSLLLACTRKTCDPEAFPTFVAPKKNKAKN------- 192

Query: 192 LPRVFSYVCDFVSNFRWSIMK---NTFVEDEPTPI---KPEGESMQLV-KPIA-ISTMTF 251
                  VC  +  + W I++   +T VE   + +   +    S  ++ KP A +S   F
Sbjct: 193 -------VCFSLVAWLWFIVRLMFHTCVEVIKSIVFICRASDTSAHIMGKPGATLSANKF 252

Query: 252 -----SLDQIKQIKNKLNASVNDVLTGIIFLGIRLYM-QEHSPESSKANSSAL------- 311
                SLD +K +KN +N +VNDVL G++  G+  Y+ Q +  E+S  +   L       
Sbjct: 253 IHRIISLDDVKMVKNAMNMTVNDVLFGMVQAGLSRYLNQRYDLETSSKSRKNLHNIGLHG 312

Query: 312 -ILLNTRKAKSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTISPLEFVKEAKAKIT 354
            +  N R  ++ + + +M+   +   WGN I ++ +P+       +   E+V++AK  + 
Sbjct: 313 VVFFNLRPNRNIEDLAKMMAKGSKCRWGNSIGYVLIPLGMKPQDDV--FEYVRQAKTIMD 359

BLAST of Sed0002925.2 vs. TAIR 10
Match: AT1G72110.2 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 128.6 bits (322), Expect = 1.0e-29
Identity = 98/356 (27.53%), Postives = 173/356 (48.60%), Query Frame = 0

Query: 12  PMSPFSQYFNTSEICVSVLGVIELESPITHSEALSIVTDVLIPANPRFTSIMVKEKCWEE 71
           P+SPF++ F+   + V  +  I  ++    S  +  + + LI  +PRF+SI+V      +
Sbjct: 13  PVSPFARLFSLPGLDVFNIVTIGCKTEGNASTIVEGIKNTLI-NHPRFSSILVTGHGEHK 72

Query: 72  RK--WKATKVNLEDHIYTPKFPMNLSLYEYDAYFDEYATKTATKPFSHSIPLWEIHIFNY 131
            K  W  TK+N+E+H+  P    N+     D + ++Y +  A  P   S PLWE H+   
Sbjct: 73  GKARWIPTKINVEEHVIVPDIDPNIE--NPDEFLEDYTSNMALSPMDMSKPLWEFHLLKL 132

Query: 132 PTSRADCCVIFKVHHSVADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQPNEVPKRGR 191
            TS A+   + + HHS+ DG  LM+ LL+C ++  DP    TF + K+++  N       
Sbjct: 133 KTSHAEAVTVARFHHSLGDGMSLMSLLLACTRKTCDPEAFPTFVAPKKNKAKN------- 192

Query: 192 LPRVFSYVCDFVSNFRWSIMK---NTFVEDEPTPI---KPEGESMQLV-KPIA-ISTMTF 251
                  VC  +  + W I++   +T VE   + +   +    S  ++ KP A +S   F
Sbjct: 193 -------VCFSLVAWLWFIVRLMFHTCVEVIKSIVFICRASDTSAHIMGKPGATLSANKF 252

Query: 252 -----SLDQIKQIKNKLNASVNDVLTGIIFLGIRLYM-QEHSPESSKANSSAL------- 311
                SLD +K +KN +N +VNDVL G++  G+  Y+ Q +  E+S  +   L       
Sbjct: 253 IHRIISLDDVKMVKNAMNMTVNDVLFGMVQAGLSRYLNQRYDLETSSKSRKNLHNIGLHG 312

Query: 312 -ILLNTRKAKSYKSVKEMLKADTDAPWGNKIAFLPVPIPKLIDSTISPLEFVKEAK 344
            +  N R  ++ + + +M+   +   WGN I ++ +P+       +   E+V++AK
Sbjct: 313 VVFFNLRPNRNIEDLAKMMAKGSKCRWGNSIGYVLIPLGMKPQDDV--FEYVRQAK 349

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147882.11.5e-16378.13O-acyltransferase WSD1-like [Momordica charantia] >XP_022147883.1 O-acyltransfer... [more]
XP_011659709.22.8e-16278.48O-acyltransferase WSD1 [Cucumis sativus] >KGN43701.2 hypothetical protein Csa_01... [more]
XP_022952997.11.3e-15676.78O-acyltransferase WSD1-like [Cucurbita moschata][more]
XP_038887587.14.8e-15475.59O-acyltransferase WSD1-like isoform X2 [Benincasa hispida][more]
XP_038887586.14.8e-15475.59O-acyltransferase WSD1-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9M3B26.3e-3227.51Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3... [more]
Q5KS418.2e-3228.54Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=... [more]
Q9M3B34.1e-3126.90Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3... [more]
F4IU145.0e-2925.00Wax ester synthase/diacylglycerol acyltransferase 3 OS=Arabidopsis thaliana OX=3... [more]
Q9C7H41.4e-2827.32Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1D2I77.1e-16478.13Diacylglycerol O-acyltransferase OS=Momordica charantia OX=3673 GN=LOC111016711 ... [more]
A0A6J1GLZ36.5e-15776.78Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455512 P... [more]
A0A0A0K2E96.7e-13878.68Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051330 PE=4... [more]
A0A5D3C6U28.2e-13678.37Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7SPR91.8e-13578.06Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
AT3G49200.14.4e-3327.51O-acyltransferase (WSD1-like) family protein [more]
AT5G53390.15.8e-3328.54O-acyltransferase (WSD1-like) family protein [more]
AT3G49190.12.9e-3226.90O-acyltransferase (WSD1-like) family protein [more]
AT1G72110.11.0e-2927.32O-acyltransferase (WSD1-like) family protein [more]
AT1G72110.21.0e-2927.53O-acyltransferase (WSD1-like) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009721O-acyltransferase WSD1, C-terminalPFAMPF06974WS_DGAT_Ccoord: 314..373
e-value: 2.8E-5
score: 24.1
IPR004255O-acyltransferase, WSD1, N-terminalPFAMPF03007WES_acyltransfcoord: 103..260
e-value: 3.1E-8
score: 33.7
IPR045034O-acyltransferase WSD1-likePANTHERPTHR31650O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEINcoord: 9..373
NoneNo IPR availablePANTHERPTHR31650:SF34O-ACYLTRANSFERASE WSD1-LIKEcoord: 9..373

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Sed0002925Sed0002925gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0002925.2-five_prime_utrSed0002925.2-five_prime_utr-LG04:43727515..43727548five_prime_UTR
Sed0002925.2-five_prime_utrSed0002925.2-five_prime_utr-LG04:43727631..43727658five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0002925.2-exonSed0002925.2-exon-LG04:43727515..43727548exon
Sed0002925.2-exonSed0002925.2-exon-LG04:43727631..43727847exon
Sed0002925.2-exonSed0002925.2-exon-LG04:43729510..43730085exon
Sed0002925.2-exonSed0002925.2-exon-LG04:43730918..43731475exon
Sed0002925.2-exonSed0002925.2-exon-LG04:43731785..43732030exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0002925.2-cdsSed0002925.2-cds-LG04:43727659..43727847CDS
Sed0002925.2-cdsSed0002925.2-cds-LG04:43729510..43730085CDS
Sed0002925.2-cdsSed0002925.2-cds-LG04:43730918..43731283CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0002925.2-three_prime_utrSed0002925.2-three_prime_utr-LG04:43731284..43731475three_prime_UTR
Sed0002925.2-three_prime_utrSed0002925.2-three_prime_utr-LG04:43731785..43732030three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Sed0002925.2Sed0002925.2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045017 glycerolipid biosynthetic process
biological_process GO:0019432 triglyceride biosynthetic process
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004144 diacylglycerol O-acyltransferase activity
molecular_function GO:0008374 O-acyltransferase activity