Sed0002074.1 (mRNA) Chayote v1

BLAST of Sed0002074.1 vs. NCBI nr
Match: XP_031745292.1 (cation/H(+) antiporter 28 isoform X1 [Cucumis sativus])

HSP 1 Score: 1366.7 bits (3536), Expect = 0.0e+00
Identity = 689/791 (87.10%), Postives = 742/791 (93.81%), Query Frame = 0

Query: 1   MARTRVHEPLRALANFNKCTIRLTEIFGRATRFILGFFIMVCLCNGIHYFLKPFSQPQIS 60
           MA+TR HEP R+LAN + CTIRLTEIFG+ATR ILGFFIM+ LCNG+HY L+PFSQP+I+
Sbjct: 38  MAKTRNHEPSRSLAN-DTCTIRLTEIFGKATRSILGFFIMMGLCNGVHYLLRPFSQPRIT 97

Query: 61  SDTIVGLVVGNLIRKQLELPTVKTLRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVA 120
           SDTIVGLVVGN IRKQL++ T+KTLRY+VDF M+CYMFVLGLE+DPYV+FKAPTRDAKVA
Sbjct: 98  SDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVIFKAPTRDAKVA 157

Query: 121 YAGMISTLILACSITPFISFSKTKEISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGR 180
           YAGMISTLILACSI PFI   K+KEISFILSLS VLSSTASPVLTR ITSLKIGKSDIGR
Sbjct: 158 YAGMISTLILACSIIPFIGMIKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGR 217

Query: 181 LVIAAGMHSDFISTLIICIGYLFCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINW 240
           LVIAAGMHSDFISTL+IC+GYLFCKCQETRIS IKGFQLG  LLIQ++  AKVSP+F+NW
Sbjct: 218 LVIAAGMHSDFISTLMICVGYLFCKCQETRISFIKGFQLGILLLIQAVLTAKVSPVFMNW 277

Query: 241 VNTENPDGKPMKGPHLVLAVAFMAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVG 300
           VN ENP+GKPMKGPHLVLAVAFMAFLC CPT+FGYNPILSAFLAGTFLPREGRVSRWA+G
Sbjct: 278 VNNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIG 337

Query: 301 KINYLLTTVFYPIFFFWMGVESKLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAIL 360
           KINYLLTTVFYPIFFFWMGVESKL+DFEP QIMTWVRLILLFAIAT+GKVVGTVIAGAIL
Sbjct: 338 KINYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVRLILLFAIATLGKVVGTVIAGAIL 397

Query: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHI 420
           GFHWPESVALGLLLTMKGHFHIYLAIAA+TAGK TTSTSIVMVI IFFTIVHAPKVVAHI
Sbjct: 398 GFHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSIVMVIVIFFTIVHAPKVVAHI 457

Query: 421 IKRARKRTPAHRMALQLLDPSSDLKLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTD 480
           I+RARKRTP HRMALQLLDPSS+LK+LLC+HGPQNT AAINIME+SRG ANPGV+VYVTD
Sbjct: 458 IQRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTD 517

Query: 481 MIELTDEIAATLVQGEGVDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALST 540
           MIELTDEIA+TLVQGEGVDS+TVTHTGVT +R+Q+T+AVQSYVDEN EGITLRRMLALST
Sbjct: 518 MIELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRMLALST 577

Query: 541 LNSMAHDICILAEESMGALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILV 600
            NSMA DICILAEE MGALIILPFHK+QR DGSL+EG T FRYVNRKVL++APCSVGILV
Sbjct: 578 FNSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQTAFRYVNRKVLRHAPCSVGILV 637

Query: 601 DRGLGTVEKISRLCVSQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAEN 660
           DRGLG+VEKISR  VSQNVAVIFIGGKDDREAL YAGRV+RHPGVKLSVIRFLVDADA N
Sbjct: 638 DRGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAVN 697

Query: 661 AARRAGTYRVSVAEQEKEMKLDDECFARFYEQHVAGKQVAYIEKHLASSSEMYSTLKSLE 720
           AARRAGTYR+SVAEQE+EM+ DDECFA FYE+HVAG  VAY+EKHLASSSE YSTLKSLE
Sbjct: 698 AARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLE 757

Query: 721 GQYALIIVGRGGKANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKGE 780
           GQYALIIVGRGGK NTVLTFGMNDWQQCPELGPIGD+LSGSEF VRTSVLII QHNLKGE
Sbjct: 758 GQYALIIVGRGGKVNTVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGE 817

Query: 781 LDGLDDDFSIM 792
           LDGLDDDFSIM
Sbjct: 818 LDGLDDDFSIM 827

BLAST of Sed0002074.1 vs. NCBI nr
Match: XP_004136122.1 (cation/H(+) antiporter 28 isoform X2 [Cucumis sativus] >XP_031745293.1 cation/H(+) antiporter 28 isoform X2 [Cucumis sativus] >KGN45116.1 hypothetical protein Csa_016064 [Cucumis sativus])

HSP 1 Score: 1366.7 bits (3536), Expect = 0.0e+00
Identity = 689/791 (87.10%), Postives = 742/791 (93.81%), Query Frame = 0

Query: 1   MARTRVHEPLRALANFNKCTIRLTEIFGRATRFILGFFIMVCLCNGIHYFLKPFSQPQIS 60
           MA+TR HEP R+LAN + CTIRLTEIFG+ATR ILGFFIM+ LCNG+HY L+PFSQP+I+
Sbjct: 1   MAKTRNHEPSRSLAN-DTCTIRLTEIFGKATRSILGFFIMMGLCNGVHYLLRPFSQPRIT 60

Query: 61  SDTIVGLVVGNLIRKQLELPTVKTLRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVA 120
           SDTIVGLVVGN IRKQL++ T+KTLRY+VDF M+CYMFVLGLE+DPYV+FKAPTRDAKVA
Sbjct: 61  SDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVIFKAPTRDAKVA 120

Query: 121 YAGMISTLILACSITPFISFSKTKEISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGR 180
           YAGMISTLILACSI PFI   K+KEISFILSLS VLSSTASPVLTR ITSLKIGKSDIGR
Sbjct: 121 YAGMISTLILACSIIPFIGMIKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGR 180

Query: 181 LVIAAGMHSDFISTLIICIGYLFCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINW 240
           LVIAAGMHSDFISTL+IC+GYLFCKCQETRIS IKGFQLG  LLIQ++  AKVSP+F+NW
Sbjct: 181 LVIAAGMHSDFISTLMICVGYLFCKCQETRISFIKGFQLGILLLIQAVLTAKVSPVFMNW 240

Query: 241 VNTENPDGKPMKGPHLVLAVAFMAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVG 300
           VN ENP+GKPMKGPHLVLAVAFMAFLC CPT+FGYNPILSAFLAGTFLPREGRVSRWA+G
Sbjct: 241 VNNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIG 300

Query: 301 KINYLLTTVFYPIFFFWMGVESKLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAIL 360
           KINYLLTTVFYPIFFFWMGVESKL+DFEP QIMTWVRLILLFAIAT+GKVVGTVIAGAIL
Sbjct: 301 KINYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVRLILLFAIATLGKVVGTVIAGAIL 360

Query: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHI 420
           GFHWPESVALGLLLTMKGHFHIYLAIAA+TAGK TTSTSIVMVI IFFTIVHAPKVVAHI
Sbjct: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSIVMVIVIFFTIVHAPKVVAHI 420

Query: 421 IKRARKRTPAHRMALQLLDPSSDLKLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTD 480
           I+RARKRTP HRMALQLLDPSS+LK+LLC+HGPQNT AAINIME+SRG ANPGV+VYVTD
Sbjct: 421 IQRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTD 480

Query: 481 MIELTDEIAATLVQGEGVDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALST 540
           MIELTDEIA+TLVQGEGVDS+TVTHTGVT +R+Q+T+AVQSYVDEN EGITLRRMLALST
Sbjct: 481 MIELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRMLALST 540

Query: 541 LNSMAHDICILAEESMGALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILV 600
            NSMA DICILAEE MGALIILPFHK+QR DGSL+EG T FRYVNRKVL++APCSVGILV
Sbjct: 541 FNSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQTAFRYVNRKVLRHAPCSVGILV 600

Query: 601 DRGLGTVEKISRLCVSQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAEN 660
           DRGLG+VEKISR  VSQNVAVIFIGGKDDREAL YAGRV+RHPGVKLSVIRFLVDADA N
Sbjct: 601 DRGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAVN 660

Query: 661 AARRAGTYRVSVAEQEKEMKLDDECFARFYEQHVAGKQVAYIEKHLASSSEMYSTLKSLE 720
           AARRAGTYR+SVAEQE+EM+ DDECFA FYE+HVAG  VAY+EKHLASSSE YSTLKSLE
Sbjct: 661 AARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLE 720

Query: 721 GQYALIIVGRGGKANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKGE 780
           GQYALIIVGRGGK NTVLTFGMNDWQQCPELGPIGD+LSGSEF VRTSVLII QHNLKGE
Sbjct: 721 GQYALIIVGRGGKVNTVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGE 780

Query: 781 LDGLDDDFSIM 792
           LDGLDDDFSIM
Sbjct: 781 LDGLDDDFSIM 790

BLAST of Sed0002074.1 vs. NCBI nr
Match: XP_038896873.1 (cation/H(+) antiporter 28 isoform X2 [Benincasa hispida])

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 685/791 (86.60%), Postives = 742/791 (93.81%), Query Frame = 0

Query: 1   MARTRVHEPLRALANFNKCTIRLTEIFGRATRFILGFFIMVCLCNGIHYFLKPFSQPQIS 60
           MARTR  EP R+LAN   CTIRLTEIFG+ATR ILGFF+MV LCNG+HY L+PFSQP+I+
Sbjct: 37  MARTRDREPSRSLANAT-CTIRLTEIFGKATRSILGFFLMVGLCNGVHYLLRPFSQPRIT 96

Query: 61  SDTIVGLVVGNLIRKQLELPTVKTLRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVA 120
           SDTIVGLV+GN IRKQL++ T+KTLRY+VDF MICYMFVLGLE+DPYVLFKAPTRDAKVA
Sbjct: 97  SDTIVGLVIGNFIRKQLDISTIKTLRYIVDFGMICYMFVLGLEVDPYVLFKAPTRDAKVA 156

Query: 121 YAGMISTLILACSITPFISFSKTKEISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGR 180
           YAGMISTLILACSI PFISF+K+KEISFI+SLS VLSSTASPVLTR ITSLKIGKSDIGR
Sbjct: 157 YAGMISTLILACSIIPFISFAKSKEISFIISLSTVLSSTASPVLTRLITSLKIGKSDIGR 216

Query: 181 LVIAAGMHSDFISTLIICIGYLFCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINW 240
           LVI AGMHSDFISTL+ICIGYLFCKCQETRIS+IKGFQLG  LLIQ++  AKVSP+F+NW
Sbjct: 217 LVIGAGMHSDFISTLMICIGYLFCKCQETRISLIKGFQLGILLLIQAVLTAKVSPVFMNW 276

Query: 241 VNTENPDGKPMKGPHLVLAVAFMAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVG 300
           VN ENP+GKPMKGPHLVLAVAFMAFLC CPT FGYNPILSAFLAGTFLPREGRVSRWA+G
Sbjct: 277 VNNENPEGKPMKGPHLVLAVAFMAFLCCCPTAFGYNPILSAFLAGTFLPREGRVSRWAIG 336

Query: 301 KINYLLTTVFYPIFFFWMGVESKLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAIL 360
           KINYLLTT+F+PIFF WMGVESKLSDFEP QIMTWVRLILLFAIATVGKVVGTVIAGAIL
Sbjct: 337 KINYLLTTIFFPIFFVWMGVESKLSDFEPGQIMTWVRLILLFAIATVGKVVGTVIAGAIL 396

Query: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHI 420
           GFHWPESVALGLLLTMKGHFHIYLAIAA+TAGK TTSTS+VMVI IFFT+VHAPKVVAHI
Sbjct: 397 GFHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSLVMVIVIFFTVVHAPKVVAHI 456

Query: 421 IKRARKRTPAHRMALQLLDPSSDLKLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTD 480
           I+RARKRTP HRMALQLLDPSS+LK+LLC+HGPQN+ AAINIME+SRG ANPGV+VYVTD
Sbjct: 457 IQRARKRTPTHRMALQLLDPSSELKILLCVHGPQNSAAAINIMEISRGTANPGVVVYVTD 516

Query: 481 MIELTDEIAATLVQGEGVDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALST 540
           MIELTDEIA+TLVQGEGVD++TVTHTGVT +RDQ+T+AVQSYVDEN EGITLRRMLALST
Sbjct: 517 MIELTDEIASTLVQGEGVDTVTVTHTGVTQMRDQVTSAVQSYVDENGEGITLRRMLALST 576

Query: 541 LNSMAHDICILAEESMGALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILV 600
            NSMA DICILAEE MGALIILPFHK+QR DGSL+EG + FRYVNRKVL++APCSVGILV
Sbjct: 577 FNSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQSAFRYVNRKVLRHAPCSVGILV 636

Query: 601 DRGLGTVEKISRLCVSQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAEN 660
           DRGLG+VEK+SR  VSQNVAVIFIGGKDDREAL YAGRV+RHPGVKLSVIRFLVDADAEN
Sbjct: 637 DRGLGSVEKVSRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAEN 696

Query: 661 AARRAGTYRVSVAEQEKEMKLDDECFARFYEQHVAGKQVAYIEKHLASSSEMYSTLKSLE 720
           AARRAGTYR+SVAEQE+EM+ DDECFA FYE+HVAG  VAY+EKHLASSSE YSTLKSLE
Sbjct: 697 AARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLE 756

Query: 721 GQYALIIVGRGGKANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKGE 780
           GQYALIIVGRGGK N+VLTFGMNDWQQCPELGPIGD+LSGSEF VRTSVLII QHNLKGE
Sbjct: 757 GQYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGE 816

Query: 781 LDGLDDDFSIM 792
           LDGLDDDFSIM
Sbjct: 817 LDGLDDDFSIM 826

BLAST of Sed0002074.1 vs. NCBI nr
Match: XP_038896872.1 (cation/H(+) antiporter 28 isoform X1 [Benincasa hispida])

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 685/791 (86.60%), Postives = 742/791 (93.81%), Query Frame = 0

Query: 1   MARTRVHEPLRALANFNKCTIRLTEIFGRATRFILGFFIMVCLCNGIHYFLKPFSQPQIS 60
           MARTR  EP R+LAN   CTIRLTEIFG+ATR ILGFF+MV LCNG+HY L+PFSQP+I+
Sbjct: 1   MARTRDREPSRSLANAT-CTIRLTEIFGKATRSILGFFLMVGLCNGVHYLLRPFSQPRIT 60

Query: 61  SDTIVGLVVGNLIRKQLELPTVKTLRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVA 120
           SDTIVGLV+GN IRKQL++ T+KTLRY+VDF MICYMFVLGLE+DPYVLFKAPTRDAKVA
Sbjct: 61  SDTIVGLVIGNFIRKQLDISTIKTLRYIVDFGMICYMFVLGLEVDPYVLFKAPTRDAKVA 120

Query: 121 YAGMISTLILACSITPFISFSKTKEISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGR 180
           YAGMISTLILACSI PFISF+K+KEISFI+SLS VLSSTASPVLTR ITSLKIGKSDIGR
Sbjct: 121 YAGMISTLILACSIIPFISFAKSKEISFIISLSTVLSSTASPVLTRLITSLKIGKSDIGR 180

Query: 181 LVIAAGMHSDFISTLIICIGYLFCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINW 240
           LVI AGMHSDFISTL+ICIGYLFCKCQETRIS+IKGFQLG  LLIQ++  AKVSP+F+NW
Sbjct: 181 LVIGAGMHSDFISTLMICIGYLFCKCQETRISLIKGFQLGILLLIQAVLTAKVSPVFMNW 240

Query: 241 VNTENPDGKPMKGPHLVLAVAFMAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVG 300
           VN ENP+GKPMKGPHLVLAVAFMAFLC CPT FGYNPILSAFLAGTFLPREGRVSRWA+G
Sbjct: 241 VNNENPEGKPMKGPHLVLAVAFMAFLCCCPTAFGYNPILSAFLAGTFLPREGRVSRWAIG 300

Query: 301 KINYLLTTVFYPIFFFWMGVESKLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAIL 360
           KINYLLTT+F+PIFF WMGVESKLSDFEP QIMTWVRLILLFAIATVGKVVGTVIAGAIL
Sbjct: 301 KINYLLTTIFFPIFFVWMGVESKLSDFEPGQIMTWVRLILLFAIATVGKVVGTVIAGAIL 360

Query: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHI 420
           GFHWPESVALGLLLTMKGHFHIYLAIAA+TAGK TTSTS+VMVI IFFT+VHAPKVVAHI
Sbjct: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSLVMVIVIFFTVVHAPKVVAHI 420

Query: 421 IKRARKRTPAHRMALQLLDPSSDLKLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTD 480
           I+RARKRTP HRMALQLLDPSS+LK+LLC+HGPQN+ AAINIME+SRG ANPGV+VYVTD
Sbjct: 421 IQRARKRTPTHRMALQLLDPSSELKILLCVHGPQNSAAAINIMEISRGTANPGVVVYVTD 480

Query: 481 MIELTDEIAATLVQGEGVDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALST 540
           MIELTDEIA+TLVQGEGVD++TVTHTGVT +RDQ+T+AVQSYVDEN EGITLRRMLALST
Sbjct: 481 MIELTDEIASTLVQGEGVDTVTVTHTGVTQMRDQVTSAVQSYVDENGEGITLRRMLALST 540

Query: 541 LNSMAHDICILAEESMGALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILV 600
            NSMA DICILAEE MGALIILPFHK+QR DGSL+EG + FRYVNRKVL++APCSVGILV
Sbjct: 541 FNSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQSAFRYVNRKVLRHAPCSVGILV 600

Query: 601 DRGLGTVEKISRLCVSQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAEN 660
           DRGLG+VEK+SR  VSQNVAVIFIGGKDDREAL YAGRV+RHPGVKLSVIRFLVDADAEN
Sbjct: 601 DRGLGSVEKVSRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAEN 660

Query: 661 AARRAGTYRVSVAEQEKEMKLDDECFARFYEQHVAGKQVAYIEKHLASSSEMYSTLKSLE 720
           AARRAGTYR+SVAEQE+EM+ DDECFA FYE+HVAG  VAY+EKHLASSSE YSTLKSLE
Sbjct: 661 AARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLE 720

Query: 721 GQYALIIVGRGGKANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKGE 780
           GQYALIIVGRGGK N+VLTFGMNDWQQCPELGPIGD+LSGSEF VRTSVLII QHNLKGE
Sbjct: 721 GQYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGE 780

Query: 781 LDGLDDDFSIM 792
           LDGLDDDFSIM
Sbjct: 781 LDGLDDDFSIM 790

BLAST of Sed0002074.1 vs. NCBI nr
Match: TYK10425.1 (cation/H(+) antiporter 28 [Cucumis melo var. makuwa])

HSP 1 Score: 1362.1 bits (3524), Expect = 0.0e+00
Identity = 682/791 (86.22%), Postives = 741/791 (93.68%), Query Frame = 0

Query: 1   MARTRVHEPLRALANFNKCTIRLTEIFGRATRFILGFFIMVCLCNGIHYFLKPFSQPQIS 60
           MA+TRVH+P R+LA  + CTIRLTEIFGRATR ILGFF+M+ LCNG+HY L+PFSQP+I+
Sbjct: 1   MAKTRVHQPSRSLA-IDSCTIRLTEIFGRATRSILGFFLMMVLCNGVHYLLRPFSQPRIT 60

Query: 61  SDTIVGLVVGNLIRKQLELPTVKTLRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVA 120
           SDTIVGL VGN +RKQL++ T+KTLRY+VDF M+CYMFVLGLE+DPYVLFKAPTRDAKVA
Sbjct: 61  SDTIVGLAVGNFVRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVLFKAPTRDAKVA 120

Query: 121 YAGMISTLILACSITPFISFSKTKEISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGR 180
           YAGMISTLILACSI PFISF+K+KEISFILSLS VLSSTASPVLTR ITSLKIGKSDIGR
Sbjct: 121 YAGMISTLILACSIIPFISFAKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGR 180

Query: 181 LVIAAGMHSDFISTLIICIGYLFCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINW 240
           LVIAAGMHSDFISTL+IC+GYLFCKCQETRIS++KGFQLG  LLIQ++  AKVSP+F+NW
Sbjct: 181 LVIAAGMHSDFISTLLICVGYLFCKCQETRISVMKGFQLGILLLIQAVLTAKVSPVFMNW 240

Query: 241 VNTENPDGKPMKGPHLVLAVAFMAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVG 300
           VN ENP+GKPMKGPHLVLAVAFMAFLC CPT+FGYNPILSAFLAGTFLPREGRVSRWA+G
Sbjct: 241 VNNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIG 300

Query: 301 KINYLLTTVFYPIFFFWMGVESKLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAIL 360
           KINYLLTTVFYPIFFFWMGVESKL+DFEP QIMTWV LILLFAIAT+GKVVGTVIAGAIL
Sbjct: 301 KINYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVTLILLFAIATIGKVVGTVIAGAIL 360

Query: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHI 420
           GFHWPESVALGLLLTMKGHFHIYLAIAA+TAGK TTSTS+VMVI IFFT+VHAPKVV  I
Sbjct: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSMVMVIVIFFTVVHAPKVVERI 420

Query: 421 IKRARKRTPAHRMALQLLDPSSDLKLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTD 480
           I+RARKRTP HRMALQLLDPSS+LK+LLC+HGPQNT AAINIME+SRG ANPGV+VYVTD
Sbjct: 421 IQRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTD 480

Query: 481 MIELTDEIAATLVQGEGVDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALST 540
           MIELTDEIA+TLVQGEGVDS+TVTHTGVT +R+Q+T+AVQSYVDEN EGITLRRMLALST
Sbjct: 481 MIELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRMLALST 540

Query: 541 LNSMAHDICILAEESMGALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILV 600
            NSMA DICILAEE MGALIILPFHK+QR DGSL+EG   FRYVNRKVL++APCSVGILV
Sbjct: 541 FNSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQAAFRYVNRKVLRHAPCSVGILV 600

Query: 601 DRGLGTVEKISRLCVSQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAEN 660
           DRGLG+VEKISR  VSQNVAVIFIGGKDDREAL YAGRV+RHPGVKLSVIRFLVDADAEN
Sbjct: 601 DRGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAEN 660

Query: 661 AARRAGTYRVSVAEQEKEMKLDDECFARFYEQHVAGKQVAYIEKHLASSSEMYSTLKSLE 720
           AARRAGTYR+SVAEQE EM+ DDECFA FYE+HVAG  VAY+EKHLASSSE YSTLKSLE
Sbjct: 661 AARRAGTYRISVAEQEDEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLE 720

Query: 721 GQYALIIVGRGGKANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKGE 780
           GQYALIIVGRGGK N+VLTFGMNDWQQCPELGPIGD+LSGSEF VRTSVLII QHNLKGE
Sbjct: 721 GQYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGE 780

Query: 781 LDGLDDDFSIM 792
           LDGLDDDFSIM
Sbjct: 781 LDGLDDDFSIM 790

BLAST of Sed0002074.1 vs. ExPASy Swiss-Prot
Match: Q8L709 (Cation/H(+) antiporter 28 OS=Arabidopsis thaliana OX=3702 GN=CHX28 PE=2 SV=2)

HSP 1 Score: 803.5 bits (2074), Expect = 2.1e-231
Identity = 413/775 (53.29%), Postives = 551/775 (71.10%), Query Frame = 0

Query: 34  ILGFFIMVCLCNGIHYFLKPFSQPQISSDTIVGLVVGNL--IRKQLELPTVKTLRYMVDF 93
           IL F  +  +   +HY +KP  QP +++D  +GL++GN+   R     P   TL  +++F
Sbjct: 27  ILVFIAIFVVRTLLHYLMKPLGQPYLTTDFAIGLILGNIPRFRGAFSGPYSITLNNIIEF 86

Query: 94  AMICYMFVLGLEMDPYVLFKAPTRDAKVAYAGMISTLILACSITPFISFSKTKEISFILS 153
            MIC+MFV+GLEM+P VL + PT+DA +AY  MI+T +LA   TPF+ ++KT    F L+
Sbjct: 87  GMICHMFVMGLEMNPSVLLRPPTKDAFIAYTSMITTFVLAFVTTPFLHYTKTSPYIFSLA 146

Query: 154 LSIVLSSTASPVLTRSITSLKIGKSDIGRLVIAAGMHSDFISTLIICIGYLFCKCQETRI 213
           LS++ SST SP+LTR I +LKI KSD+G+L  AAG+H+D ISTL+ C G++F   ++   
Sbjct: 147 LSLMASSTGSPILTRVIANLKIRKSDLGKLASAAGVHTDMISTLLYCFGFIFFPTEKPLA 206

Query: 214 ----SIIKGFQLGAALLIQSIFVAKVSPIFINWVNTENPDGKPMKGPHLVLAVAFMAFLC 273
                  +   +    L Q  F + VSPIF+NWVN ENP+GKP+KG HLV+++AF+  +C
Sbjct: 207 RPLHRFFRALLMFCLFLAQVTFTSIVSPIFLNWVNNENPEGKPLKGSHLVMSLAFVVLIC 266

Query: 274 SCPTM---FGYNPILSAFLAGTFLPREGRVSRWAVGKINYLLTTVFYPIFFFWMGVESKL 333
           S PT      YNPILSAF AG FLP +GR+S+W + KINYLL+TVFYPIFFFW+G    +
Sbjct: 267 SFPTWPPESMYNPILSAFTAGLFLPNKGRMSKWIINKINYLLSTVFYPIFFFWVGFIIHM 326

Query: 334 SDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAILGFHWPESVALGLLLTMKGHFHIYL 393
            +F+    M WVR   L      GKV GTV+ G +LG+H PE+ +LGLLLT KGHFH+YL
Sbjct: 327 RNFDITDKMAWVRFFSLLGTVIAGKVTGTVLCGLLLGYHVPETASLGLLLTTKGHFHVYL 386

Query: 394 AIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHIIKRARKRTPAHRMALQLLDPSSDL 453
           A  A    +  ++T  +++  I FT+V++P VV  IIKRARKR P H MALQ LDP+++L
Sbjct: 387 AALAIRTNRVKSTTGALIIFIIVFTVVYSPFVVMDIIKRARKRVPVHIMALQWLDPTTEL 446

Query: 454 KLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTDMIELTDEIAATLVQGEGV----DS 513
           ++L+ LHGP N  + +N+ME+  G   PG I Y TDM+ELTDEIAATL +G G     DS
Sbjct: 447 RILIGLHGPHNIGSTLNVMEICHGGREPGSIFYATDMVELTDEIAATLKKGGGAGQSNDS 506

Query: 514 MTVTHTGVTLIRDQITAAVQSYVD-ENREGITLRRMLALSTLNSMAHDICILAEESMGAL 573
           +TVT   VT +R+ ITAAV  Y +  N +G+T+RRMLALST  +MAHD+C LA+E M ++
Sbjct: 507 VTVTDRSVTEMRESITAAVNGYGELRNGQGVTVRRMLALSTFVTMAHDVCGLADELMVSI 566

Query: 574 IILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILVDRGLGTVEKISRLCVSQNV 633
           IILPFHK    DG+L+ GH GFR+VNRK+LKNAPCSVGILVDR  G  E+  R   S  +
Sbjct: 567 IILPFHKRLNPDGTLDAGHAGFRHVNRKILKNAPCSVGILVDRSFGQTEEAWRPGASMGI 626

Query: 634 AVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAENAARRAGTY-RVSVAEQEKE 693
           A+IFIGG+DDREAL +A +V+RHP VKL VIRFL D  ++NA +R+    R SV +QE+E
Sbjct: 627 AIIFIGGRDDREALAFAAQVARHPAVKLKVIRFLEDKSSQNAQKRSSILNRASVVDQEEE 686

Query: 694 MKLDDECFARFYEQHVA-GKQVAYIEKHLASSSEMYSTLKSLEGQYALIIVGR-GGKANT 753
           MKLDDECFA FYE+++A G +V+Y+EKHL +SSE ++ LKSL+G+Y L+IVGR GG+A++
Sbjct: 687 MKLDDECFAEFYERYIAGGGRVSYMEKHLTNSSETFTALKSLDGEYGLVIVGRGGGRASS 746

Query: 754 VLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKGELDGLDDDFSIM 792
            LT G+NDWQQCPELGPIGDVLSGS+F   TS+LIIQQ   +G+L+GL DDF+I+
Sbjct: 747 GLTTGLNDWQQCPELGPIGDVLSGSDFSHNTSMLIIQQQRTRGQLEGLHDDFTIL 801

BLAST of Sed0002074.1 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 3.3e-99
Identity = 228/758 (30.08%), Postives = 401/758 (52.90%), Query Frame = 0

Query: 33  FILGFFIMVCLCNGIHYFLKPFSQPQISSDTIVGLVVG-NLIRKQLELP-------TVKT 92
           F+L   ++V +     + LKPF QP++ S+ + G+V+G +++ +  +         +V  
Sbjct: 40  FVLQLTLVVVVTRFFVFILKPFRQPRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMV 99

Query: 93  LRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVAYAGMISTLIL--ACSITPFISFSK 152
           L  M +  ++ ++F++G+EMD  V+ K   R   +A  GM+   ++  A S +   S   
Sbjct: 100 LETMANVGLLYFLFLVGVEMDIMVVRKTGKRALTIAIGGMVLPFLIGAAFSFSMHRSEDH 159

Query: 153 TKEISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGRLVIAAGMHSDFISTLIICIGYL 212
             + ++IL L + LS TA PVL R +  LK+  ++IGR+ ++A + +D  + +++ +   
Sbjct: 160 LGQGTYILFLGVALSVTAFPVLARILAELKLINTEIGRISMSAALVNDMFAWILLALAIA 219

Query: 213 FCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINWVNTENPDGKPMKGPHLVLAVAF 272
             +  +T  + +      A  +   +FV +     I W+  + P+G+     H+ L +  
Sbjct: 220 LAESDKTSFASLWVMISSAVFIAVCVFVVRPG---IAWIIRKTPEGENFSEFHICLILTG 279

Query: 273 MAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVGKINYLLTTVFYPIFFFWMGVES 332
           +          G + +  AF+ G  +P  G +    + K+   ++ +  P+FF   G+++
Sbjct: 280 VMISGFITDAIGTHSVFGAFVFGLVIP-NGPLGLTLIEKLEDFVSGLLLPLFFAISGLKT 339

Query: 333 KLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAILGFHWPESVALGLLLTMKGHFHI 392
            ++  +     TW+ L L+  +A  GKV+GTVI     G    E + LGLLL  KG   +
Sbjct: 340 NIAAIQGP--ATWLTLFLVIFLACAGKVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEM 399

Query: 393 YLAIAAQTAGKTTTSTSIVMV-IAIFFTIVHAPKVVAHIIKRARKRTPAHRMALQLLDPS 452
            +    +        T   MV +A+  T V  P +V  + K  +K     R  +Q   P 
Sbjct: 400 IVLNVGKDQKVLDDETFATMVLVALVMTGVITP-IVTILYKPVKKSVSYKRRTIQQTKPD 459

Query: 453 SDLKLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTDMIELTDEIAATLVQGEGVDSM 512
           S+L++L+C+H P+N    IN++E S       + +YV  ++ELT   +A L+      S 
Sbjct: 460 SELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIYVLHLVELTGRASAMLIVHNTRKSG 519

Query: 513 TVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALSTLNSMAHDICILAEESMGALII 572
                      D I  A ++Y +++   + ++ + A+S  ++M  D+C LAE+   + II
Sbjct: 520 RPALNRTQAQSDHIINAFENY-EQHAAFVAVQPLTAISPYSTMHEDVCSLAEDKRVSFII 579

Query: 573 LPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILVDRGLGTVEKISRLCVSQNVAV 632
           +PFHK Q  DG +   +  +R VN+ +L+N+PCSVGILVDRGL    +++   VS  VAV
Sbjct: 580 IPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVGILVDRGLNGATRLNSNTVSLQVAV 639

Query: 633 IFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDAD-AENAARRA---GTYRVSVAEQEK 692
           +F GG DDREAL YA R+++HPG+ L+V+RF+ D D A+ A+ RA      ++   +  K
Sbjct: 640 LFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDEDEADTASTRATNDSDLKIPKMDHRK 699

Query: 693 EMKLDDECFARFYEQHVAGKQVAYIEKHLASSSEMYSTLKSLEGQYALIIVGRGGKANTV 752
           + +LDD+    F  ++   + + YIEK +++  E  + ++S++  + L IVGRG   ++ 
Sbjct: 700 QRQLDDDYINLFRAENAEYESIVYIEKLVSNGEETVAAVRSMDSSHDLFIVGRGEGMSSP 759

Query: 753 LTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQH 776
           LT G+ DW +CPELG IGD+L+ S+F    SVL++QQ+
Sbjct: 760 LTAGLTDWSECPELGAIGDLLASSDFAATVSVLVVQQY 789

BLAST of Sed0002074.1 vs. ExPASy Swiss-Prot
Match: Q9FFR9 (Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.6e-85
Identity = 227/771 (29.44%), Postives = 402/771 (52.14%), Query Frame = 0

Query: 34  ILGFFIMVCLCNGIHYFLKPFSQPQISSDTIVGLVVGN--LIRKQLELPTV------KTL 93
           IL   I++ L   + Y L+P  QP++ ++ I G+++G   L R +  L  V        L
Sbjct: 35  ILQIVIVIVLTRVLAYLLRPLRQPRVIAEVIGGIMLGPSLLGRSKAFLDAVFPKKSLTVL 94

Query: 94  RYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVAYAGMISTLILACSITPFISFSKTKE 153
             + +  ++ ++F+ GLE+D   L +   +   +A AG+     L    +  +  + +K 
Sbjct: 95  ETLANLGLLFFLFLAGLEIDTKALRRTGKKALGIALAGITLPFALGIGSSFVLKATISKG 154

Query: 154 I---SFILSLSIVLSSTASPVLTRSITSLKIGKSDIGRLVIAAGMHSDFISTLIICIGYL 213
           +   +F++ + + LS TA PVL R +  LK+  ++IGRL ++A   +D  + +++ +   
Sbjct: 155 VNSTAFLVFMGVALSITAFPVLARILAELKLLTTEIGRLAMSAAAVNDVAAWILLALAIA 214

Query: 214 FCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINWVNTENPDGKPMKGPHLVLAVAF 273
                 + +  +  F  G A +I + F+  + PIF  W++    +G+P++  ++   +A 
Sbjct: 215 LSGSNTSPLVSLWVFLSGCAFVIGASFI--IPPIF-RWISRRCHEGEPIEETYICATLAV 274

Query: 274 MAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVGKINYLLTTVFYPIFFFWMGVES 333
           +          G + +  AF+ G  +P+EG  +   V K+  L++ +F P++F   G+++
Sbjct: 275 VLVCGFITDAIGIHSMFGAFVVGVLIPKEGPFAGALVEKVEDLVSGLFLPLYFVASGLKT 334

Query: 334 KLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAILGFHWP--ESVALGLLLTMKGHF 393
            ++  +  Q  +W  L+L+ A A  GK++GT+  G  L F  P  E++ LG L+  KG  
Sbjct: 335 NVATIQGAQ--SWGLLVLVTATACFGKILGTL--GVSLAFKIPMREAITLGFLMNTKGLV 394

Query: 394 H-IYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVA--HIIKRARKRTPAHRMALQL 453
             I L I          + +I++++A+F T +  P V+A     +RA+K       A++ 
Sbjct: 395 ELIVLNIGKDRKVLNDQTFAIMVLMALFTTFITTPVVMAVYKPARRAKKEGEYKHRAVER 454

Query: 454 LDPSSDLKLLLCLHGPQNTTAAINIMELSRG-RANPGVIVYVTDMIELTDEIAATL---- 513
            + ++ L++L C HG  +  + IN++E SRG     G+ VY   + EL++  +A L    
Sbjct: 455 ENTNTQLRILTCFHGAGSIPSMINLLEASRGIEKGEGLCVYALHLRELSERSSAILMVHK 514

Query: 514 VQGEGVDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALSTLNSMAHDICILA 573
           V+  G+        GV    DQ+  A Q++   +R  + +R M A+S+++ +  DIC  A
Sbjct: 515 VRKNGMPFW--NRRGVNADADQVVVAFQAFQQLSR--VNVRPMTAISSMSDIHEDICTTA 574

Query: 574 EESMGALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILVDRGLGTVEKISR 633
                A++ILPFHK Q+ DGSL      +R+VNR+VL  APCSVGI VDRGLG   ++S 
Sbjct: 575 VRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRVLLQAPCSVGIFVDRGLGGSSQVSA 634

Query: 634 LCVSQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAENAARRAGTYRVSV 693
             VS +V V+F GG DDREAL Y  R++ HPG+ L+V RF+V  +             + 
Sbjct: 635 QDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVLTVFRFVVSPERVGEIVNVEVSNNNN 694

Query: 694 AEQE-KEMKLDDECFARFYEQHVAGKQVAYIEKHLASSS-EMYSTLKSLEGQYALIIVGR 753
             Q  K +K D+E  +   +     + V ++EK + +++ ++ S ++ +  +  L +VGR
Sbjct: 695 ENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQIENAAVDVRSAIEEVR-RSNLFLVGR 754

Query: 754 --GGKANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKG 780
             GG+    +   + +  +CPELGP+G +L   E   + SVL+IQQ+N  G
Sbjct: 755 MPGGE----IALAIRENSECPELGPVGSLLISPESSTKASVLVIQQYNGTG 789

BLAST of Sed0002074.1 vs. ExPASy Swiss-Prot
Match: Q9LUN4 (Cation/H(+) antiporter 19 OS=Arabidopsis thaliana OX=3702 GN=CHX19 PE=2 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 3.7e-82
Identity = 222/757 (29.33%), Postives = 387/757 (51.12%), Query Frame = 0

Query: 34  ILGFFIMVCLCNGIHYFLKPFSQPQISSDTIVGLVVG--NLIRKQLELPTV------KTL 93
           IL   ++V     + YFLKP  QP++ ++ I G+++G   L R +  L T+        L
Sbjct: 36  ILQIVLVVVFTRLLAYFLKPLKQPRVIAEIIGGILLGPSALGRSKAYLDTIFPKKSLTVL 95

Query: 94  RYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVAYAGMISTLILACSITPFISFSKTK- 153
             + +  ++ ++F++GLE+D   + K   +   +A AG+    I+    +  +S + +K 
Sbjct: 96  DTLANIGLLFFLFLVGLELDFAAIKKTGKKSLLIAIAGISLPFIVGVGTSFVLSATISKG 155

Query: 154 --EISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGRLVIAAGMHSDFISTLIICIGYL 213
             ++ FI+ + + LS TA PVL R +  LK+  +DIGR+ ++A   +D  + +++ +   
Sbjct: 156 VDQLPFIVFMGVALSITAFPVLARILAELKLLTTDIGRMAMSAAGVNDVAAWILLALAIA 215

Query: 214 FCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINWVNTENPDGKPMKGPHLVLAVAF 273
                 + +  +     G   +I ++   K     + ++    P+G+P+K  ++ + +  
Sbjct: 216 LSGDGTSPLVSVWVLLCGTGFVIFAVVAIKP---LLAYMARRCPEGEPVKELYVCVTLTV 275

Query: 274 MAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVGKINYLLTTVFYPIFFFWMGVES 333
           +          G + +  AF+ G   P+EG   R    KI  L++ +  P++F   G+++
Sbjct: 276 VLAASFVTDTIGIHALFGAFVVGIVAPKEGPFCRILTEKIEDLVSGLLLPLYFAASGLKT 335

Query: 334 KLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAILGFHWPESVALGLLLTMKGHFH- 393
            ++     Q  +W  L+L+      GK+VGTV +  +    + E+V LG L+  KG    
Sbjct: 336 DVTTIRGAQ--SWGLLVLVILTTCFGKIVGTVGSSMLCKVPFREAVTLGFLMNTKGLVEL 395

Query: 394 IYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHIIKRARKRTPAHRMALQLLDPS 453
           I L I          + +I++++A+F T +  P +V  I K ARK  P     +Q  D  
Sbjct: 396 IVLNIGKDRKVLNDQAFAILVLMALFTTFITTP-IVMLIYKPARKGAPYKHRTIQRKDHD 455

Query: 454 SDLKLLLCLHGPQNTTAAINIMELSRGRANPG-VIVYVTDMIELTDEIAATLVQGEGVDS 513
           S+L++L C H  +N    IN++E SRG    G + VY   ++EL++  +A  +  +  ++
Sbjct: 456 SELRILACFHSTRNIPTLINLIESSRGTGKKGRLCVYAMHLMELSERSSAIAMVHKARNN 515

Query: 514 MTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALSTLNSMAHDICILAEESMGALI 573
                  +    DQ+  A ++Y  ++   + +R M A+S L+S+  DIC  A +   A+I
Sbjct: 516 GLPIWNKIERSTDQMVIAFEAY--QHLRAVAVRPMTAISGLSSIHEDICTSAHQKRVAMI 575

Query: 574 ILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILVDRGLGTVEKISRLCVSQNVA 633
           +LPFHK QR DG++      F  VN++VL+ APCSVGILVDRGLG   ++    V+  V 
Sbjct: 576 LLPFHKHQRMDGAMESIGHRFHEVNQRVLQRAPCSVGILVDRGLGGTSQVVASEVAYKVV 635

Query: 634 VIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAENAARRAGTYRVSVAEQEKEMK 693
           + F GG DDREAL Y  ++  HPG+ L+V +F+    A    +R    +    E+EK+ K
Sbjct: 636 IPFFGGLDDREALAYGMKMVEHPGITLTVYKFVA---ARGTLKR--FEKSEHDEKEKKEK 695

Query: 694 LDDECFARFYEQHVAGKQ-VAYIEKHLASSSEMYSTLKSLEGQYALIIVGRGGKANTVLT 753
             DE F R       G + +AY E+ + S  ++ +TLKS+  +  L +VGR     +++ 
Sbjct: 696 ETDEEFVRELMNDPRGNESLAYEERVVESKDDIIATLKSM-SKCNLFVVGRNAAVASLVK 755

Query: 754 FGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHN 777
                   CPELGP+G +LS SEF    SVL++Q ++
Sbjct: 756 S-----TDCPELGPVGRLLSSSEFSTTASVLVVQGYD 773

BLAST of Sed0002074.1 vs. ExPASy Swiss-Prot
Match: Q8VYD4 (Cation/H(+) antiporter 23, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CHX23 PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.4e-68
Identity = 200/763 (26.21%), Postives = 361/763 (47.31%), Query Frame = 0

Query: 33  FILGFFIMVCLCNGIHYFLKPFSQPQISSDTIVGL-----VVGN---LIRKQLELPTVKT 92
           FI   F+       ++Y  +P   P   +  + GL     V+GN   +I           
Sbjct: 54  FITQLFVANLSYRVLYYLTRPLYLPPFVAQILCGLLFSPSVLGNTRFIIAHVFPYRFTMV 113

Query: 93  LRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVAYAGMISTLILACSITPFISF---- 152
           L    + A++  +F+LGL MD  ++     +   +A+ G    L++A  +  F+ +    
Sbjct: 114 LETFANLALVYNIFLLGLGMDLRMVRITELKPVIIAFTG----LLVALPVGAFLYYLPGN 173

Query: 153 -SKTKEISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGRLVIAAGMHSDFISTLIICI 212
               K IS  +  S+ L+ T  P L R +  LK+ +SD+GR  + A + +D  + +++  
Sbjct: 174 GHPDKIISGCVFWSVALACTNFPDLARILADLKLLRSDMGRTAMCAAIVTDLCTWVLLVF 233

Query: 213 GYLFCKCQETRISIIKGFQLGAAL-LIQSIFVAKVSPIFINWVNTENPDGKPMKGPHLVL 272
           G+       T   ++    +  A+ ++  IFV +     I W+  +      +   H+  
Sbjct: 234 GFASFSKSGTWNKMMPFVIITTAIFVLLCIFVIRPG---IAWIFAKTVKAGHVGDTHVWF 293

Query: 273 AVAFMAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVGKINYLLTTVFYPIFFFWM 332
            +  +          G + I  AFL G  +P +  +      K++  L+ +  P+F+   
Sbjct: 294 ILGGVVLCGLITDACGVHSITGAFLFGLSIPHDHIIRNMIEEKLHDFLSGILMPLFYIIC 353

Query: 333 GVESKLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAILGFHWPESVALGLLLTMKG 392
           G+ + +      Q      ++++   + + K+V TVI    +     ++ A+G L+  KG
Sbjct: 354 GLRADIGFM--LQFTDKFMMVVVICSSFLVKIVTTVITSLFMHIPMRDAFAIGALMNTKG 413

Query: 393 HFHIYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHIIKRARKRTPAHRMALQLL 452
              + +  A +      +     M IA+    +    ++A   K  +K        +Q +
Sbjct: 414 TLSLVVLNAGRDTKALDSPMYTHMTIALLVMSLVVEPLLAFAYKPKKKLAHYKHRTVQKI 473

Query: 453 DPSSDLKLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTDMIELTDEIAAT-LVQGEG 512
              ++L++L C+H   N +   N++++S       + V+   ++ELT    A+ L+  + 
Sbjct: 474 KGETELRVLACVHVLPNVSGITNLLQVSNATKQSPLSVFAIHLVELTGRTTASLLIMNDE 533

Query: 513 VDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALSTLNSMAHDICILAEESMG 572
                     V    DQI    ++ ++ N + +T++ + A+S   +M  DIC+LAE+   
Sbjct: 534 CKPKANFSDRVRAESDQIAETFEA-MEVNNDAMTVQTITAVSPYATMHEDICVLAEDKRV 593

Query: 573 ALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILVDRGLGTV--EKISRLCV 632
             IILP+HK    DG + EG++    +N+ VL +APCSVGILVDRG+  V  E      +
Sbjct: 594 CFIILPYHKHLTPDGRMGEGNSSHAEINQNVLSHAPCSVGILVDRGMAMVRSESFRGESM 653

Query: 633 SQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAENAARRAGTYRVSVAEQ 692
            + VA++F+GG DDREAL YA R+     +KL+V+RF+   +A  ++ +        AE 
Sbjct: 654 KREVAMLFVGGPDDREALSYAWRMVGQHVIKLTVVRFVPGREALISSGKV------AAEY 713

Query: 693 EKEMKLDDECFARFYEQHVAGKQVAYIEKHLASSSEMYSTLKSLE--GQYALIIVGRGGK 752
           E+E ++DDEC   F  + +    V YIEK +    +  +T++ +E    Y L +VGRG  
Sbjct: 714 EREKQVDDECIYEFNFKTMNDSSVKYIEKVVNDGQDTIATIREMEDNNSYDLYVVGRGYN 773

Query: 753 ANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHN 777
           +++ +T G+NDW   PELG IGD L+ S F +  SVL+IQQ++
Sbjct: 774 SDSPVTAGLNDWSSSPELGTIGDTLASSNFTMHASVLVIQQYS 800

BLAST of Sed0002074.1 vs. ExPASy TrEMBL
Match: A0A0A0KBF5 (Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G426570 PE=4 SV=1)

HSP 1 Score: 1366.7 bits (3536), Expect = 0.0e+00
Identity = 689/791 (87.10%), Postives = 742/791 (93.81%), Query Frame = 0

Query: 1   MARTRVHEPLRALANFNKCTIRLTEIFGRATRFILGFFIMVCLCNGIHYFLKPFSQPQIS 60
           MA+TR HEP R+LAN + CTIRLTEIFG+ATR ILGFFIM+ LCNG+HY L+PFSQP+I+
Sbjct: 1   MAKTRNHEPSRSLAN-DTCTIRLTEIFGKATRSILGFFIMMGLCNGVHYLLRPFSQPRIT 60

Query: 61  SDTIVGLVVGNLIRKQLELPTVKTLRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVA 120
           SDTIVGLVVGN IRKQL++ T+KTLRY+VDF M+CYMFVLGLE+DPYV+FKAPTRDAKVA
Sbjct: 61  SDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVIFKAPTRDAKVA 120

Query: 121 YAGMISTLILACSITPFISFSKTKEISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGR 180
           YAGMISTLILACSI PFI   K+KEISFILSLS VLSSTASPVLTR ITSLKIGKSDIGR
Sbjct: 121 YAGMISTLILACSIIPFIGMIKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGR 180

Query: 181 LVIAAGMHSDFISTLIICIGYLFCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINW 240
           LVIAAGMHSDFISTL+IC+GYLFCKCQETRIS IKGFQLG  LLIQ++  AKVSP+F+NW
Sbjct: 181 LVIAAGMHSDFISTLMICVGYLFCKCQETRISFIKGFQLGILLLIQAVLTAKVSPVFMNW 240

Query: 241 VNTENPDGKPMKGPHLVLAVAFMAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVG 300
           VN ENP+GKPMKGPHLVLAVAFMAFLC CPT+FGYNPILSAFLAGTFLPREGRVSRWA+G
Sbjct: 241 VNNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIG 300

Query: 301 KINYLLTTVFYPIFFFWMGVESKLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAIL 360
           KINYLLTTVFYPIFFFWMGVESKL+DFEP QIMTWVRLILLFAIAT+GKVVGTVIAGAIL
Sbjct: 301 KINYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVRLILLFAIATLGKVVGTVIAGAIL 360

Query: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHI 420
           GFHWPESVALGLLLTMKGHFHIYLAIAA+TAGK TTSTSIVMVI IFFTIVHAPKVVAHI
Sbjct: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSIVMVIVIFFTIVHAPKVVAHI 420

Query: 421 IKRARKRTPAHRMALQLLDPSSDLKLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTD 480
           I+RARKRTP HRMALQLLDPSS+LK+LLC+HGPQNT AAINIME+SRG ANPGV+VYVTD
Sbjct: 421 IQRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTD 480

Query: 481 MIELTDEIAATLVQGEGVDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALST 540
           MIELTDEIA+TLVQGEGVDS+TVTHTGVT +R+Q+T+AVQSYVDEN EGITLRRMLALST
Sbjct: 481 MIELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRMLALST 540

Query: 541 LNSMAHDICILAEESMGALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILV 600
            NSMA DICILAEE MGALIILPFHK+QR DGSL+EG T FRYVNRKVL++APCSVGILV
Sbjct: 541 FNSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQTAFRYVNRKVLRHAPCSVGILV 600

Query: 601 DRGLGTVEKISRLCVSQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAEN 660
           DRGLG+VEKISR  VSQNVAVIFIGGKDDREAL YAGRV+RHPGVKLSVIRFLVDADA N
Sbjct: 601 DRGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAVN 660

Query: 661 AARRAGTYRVSVAEQEKEMKLDDECFARFYEQHVAGKQVAYIEKHLASSSEMYSTLKSLE 720
           AARRAGTYR+SVAEQE+EM+ DDECFA FYE+HVAG  VAY+EKHLASSSE YSTLKSLE
Sbjct: 661 AARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLE 720

Query: 721 GQYALIIVGRGGKANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKGE 780
           GQYALIIVGRGGK NTVLTFGMNDWQQCPELGPIGD+LSGSEF VRTSVLII QHNLKGE
Sbjct: 721 GQYALIIVGRGGKVNTVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGE 780

Query: 781 LDGLDDDFSIM 792
           LDGLDDDFSIM
Sbjct: 781 LDGLDDDFSIM 790

BLAST of Sed0002074.1 vs. ExPASy TrEMBL
Match: A0A5D3CFY8 (Cation/H(+) antiporter 28 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00710 PE=4 SV=1)

HSP 1 Score: 1362.1 bits (3524), Expect = 0.0e+00
Identity = 682/791 (86.22%), Postives = 741/791 (93.68%), Query Frame = 0

Query: 1   MARTRVHEPLRALANFNKCTIRLTEIFGRATRFILGFFIMVCLCNGIHYFLKPFSQPQIS 60
           MA+TRVH+P R+LA  + CTIRLTEIFGRATR ILGFF+M+ LCNG+HY L+PFSQP+I+
Sbjct: 1   MAKTRVHQPSRSLA-IDSCTIRLTEIFGRATRSILGFFLMMVLCNGVHYLLRPFSQPRIT 60

Query: 61  SDTIVGLVVGNLIRKQLELPTVKTLRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVA 120
           SDTIVGL VGN +RKQL++ T+KTLRY+VDF M+CYMFVLGLE+DPYVLFKAPTRDAKVA
Sbjct: 61  SDTIVGLAVGNFVRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVLFKAPTRDAKVA 120

Query: 121 YAGMISTLILACSITPFISFSKTKEISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGR 180
           YAGMISTLILACSI PFISF+K+KEISFILSLS VLSSTASPVLTR ITSLKIGKSDIGR
Sbjct: 121 YAGMISTLILACSIIPFISFAKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGR 180

Query: 181 LVIAAGMHSDFISTLIICIGYLFCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINW 240
           LVIAAGMHSDFISTL+IC+GYLFCKCQETRIS++KGFQLG  LLIQ++  AKVSP+F+NW
Sbjct: 181 LVIAAGMHSDFISTLLICVGYLFCKCQETRISVMKGFQLGILLLIQAVLTAKVSPVFMNW 240

Query: 241 VNTENPDGKPMKGPHLVLAVAFMAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVG 300
           VN ENP+GKPMKGPHLVLAVAFMAFLC CPT+FGYNPILSAFLAGTFLPREGRVSRWA+G
Sbjct: 241 VNNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIG 300

Query: 301 KINYLLTTVFYPIFFFWMGVESKLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAIL 360
           KINYLLTTVFYPIFFFWMGVESKL+DFEP QIMTWV LILLFAIAT+GKVVGTVIAGAIL
Sbjct: 301 KINYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVTLILLFAIATIGKVVGTVIAGAIL 360

Query: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHI 420
           GFHWPESVALGLLLTMKGHFHIYLAIAA+TAGK TTSTS+VMVI IFFT+VHAPKVV  I
Sbjct: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSMVMVIVIFFTVVHAPKVVERI 420

Query: 421 IKRARKRTPAHRMALQLLDPSSDLKLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTD 480
           I+RARKRTP HRMALQLLDPSS+LK+LLC+HGPQNT AAINIME+SRG ANPGV+VYVTD
Sbjct: 421 IQRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTD 480

Query: 481 MIELTDEIAATLVQGEGVDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALST 540
           MIELTDEIA+TLVQGEGVDS+TVTHTGVT +R+Q+T+AVQSYVDEN EGITLRRMLALST
Sbjct: 481 MIELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRMLALST 540

Query: 541 LNSMAHDICILAEESMGALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILV 600
            NSMA DICILAEE MGALIILPFHK+QR DGSL+EG   FRYVNRKVL++APCSVGILV
Sbjct: 541 FNSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQAAFRYVNRKVLRHAPCSVGILV 600

Query: 601 DRGLGTVEKISRLCVSQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAEN 660
           DRGLG+VEKISR  VSQNVAVIFIGGKDDREAL YAGRV+RHPGVKLSVIRFLVDADAEN
Sbjct: 601 DRGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAEN 660

Query: 661 AARRAGTYRVSVAEQEKEMKLDDECFARFYEQHVAGKQVAYIEKHLASSSEMYSTLKSLE 720
           AARRAGTYR+SVAEQE EM+ DDECFA FYE+HVAG  VAY+EKHLASSSE YSTLKSLE
Sbjct: 661 AARRAGTYRISVAEQEDEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLE 720

Query: 721 GQYALIIVGRGGKANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKGE 780
           GQYALIIVGRGGK N+VLTFGMNDWQQCPELGPIGD+LSGSEF VRTSVLII QHNLKGE
Sbjct: 721 GQYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGE 780

Query: 781 LDGLDDDFSIM 792
           LDGLDDDFSIM
Sbjct: 781 LDGLDDDFSIM 790

BLAST of Sed0002074.1 vs. ExPASy TrEMBL
Match: A0A1S3CE27 (cation/H(+) antiporter 28 OS=Cucumis melo OX=3656 GN=LOC103499939 PE=4 SV=1)

HSP 1 Score: 1361.3 bits (3522), Expect = 0.0e+00
Identity = 681/791 (86.09%), Postives = 743/791 (93.93%), Query Frame = 0

Query: 1   MARTRVHEPLRALANFNKCTIRLTEIFGRATRFILGFFIMVCLCNGIHYFLKPFSQPQIS 60
           MA+TRVH+P R+LA  + CTIRLTEIFGRATR ILGFF+M+ LCNG+HY L+PFSQP+I+
Sbjct: 1   MAKTRVHQPSRSLA-IDSCTIRLTEIFGRATRSILGFFLMMVLCNGVHYLLRPFSQPRIT 60

Query: 61  SDTIVGLVVGNLIRKQLELPTVKTLRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVA 120
           SDTIVGLVVGN +RKQL++ T+KTLRY+VDF M+CYMFVLGLE+DPYVLFKAPTRDAKVA
Sbjct: 61  SDTIVGLVVGNFVRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVLFKAPTRDAKVA 120

Query: 121 YAGMISTLILACSITPFISFSKTKEISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGR 180
           YAG+ISTLILACSI PFIS++K+KEISFILSLS VLSSTASPVLTR ITSLKIGKSDIGR
Sbjct: 121 YAGLISTLILACSIIPFISYAKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGR 180

Query: 181 LVIAAGMHSDFISTLIICIGYLFCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINW 240
           LVIAAGMHSDFISTL+IC+GYLFCKCQETRIS++KGFQLG  LLIQ++  AKVSP+F+NW
Sbjct: 181 LVIAAGMHSDFISTLLICVGYLFCKCQETRISVMKGFQLGILLLIQAVLTAKVSPVFMNW 240

Query: 241 VNTENPDGKPMKGPHLVLAVAFMAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVG 300
           VN ENP+GKPMKGPHLVLAVAFMAFLC CPT+FGYNPILSAFLAGTFLPREGRVSRWA+G
Sbjct: 241 VNNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIG 300

Query: 301 KINYLLTTVFYPIFFFWMGVESKLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAIL 360
           KINYLLTTVFYPIFFFWMGVESKL+DFEP QIMTWVRL+LLFAIAT+GKVVGTVIAGAIL
Sbjct: 301 KINYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVRLMLLFAIATIGKVVGTVIAGAIL 360

Query: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHI 420
           GFHWPESVALGLLLTMKGHFHIYLAIAA+TAGK TTSTS+VMVI IFFT+VHAPKVV  I
Sbjct: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSMVMVIVIFFTVVHAPKVVECI 420

Query: 421 IKRARKRTPAHRMALQLLDPSSDLKLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTD 480
           I+RARKRTP HRMALQLLDPSS+LK+LLC+HGPQNT AAINIME+SRG ANPGV+VYVTD
Sbjct: 421 IQRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTD 480

Query: 481 MIELTDEIAATLVQGEGVDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALST 540
           MIELTDEIA+TLVQGEGVDS+TVTHTGVT +R+Q+T+AVQSYVDEN EGITLRRMLALST
Sbjct: 481 MIELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRMLALST 540

Query: 541 LNSMAHDICILAEESMGALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILV 600
            NSMA DICILAEE MGALIILPFHK+QR DGSL+EG   FRYVNRKVL++APCSVGILV
Sbjct: 541 FNSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQAAFRYVNRKVLRHAPCSVGILV 600

Query: 601 DRGLGTVEKISRLCVSQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAEN 660
           DRGLG+VEKISR  VSQNVAVIFIGGKDDREAL YAGRV+RHPGVKLSVIRFLVDADAEN
Sbjct: 601 DRGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAEN 660

Query: 661 AARRAGTYRVSVAEQEKEMKLDDECFARFYEQHVAGKQVAYIEKHLASSSEMYSTLKSLE 720
           AARRAGTYR+SVAEQE EM+ DDECFA FYE+HVAG  VAY+EKHLASSSE YSTLKSLE
Sbjct: 661 AARRAGTYRISVAEQEDEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLE 720

Query: 721 GQYALIIVGRGGKANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKGE 780
           GQYALIIVGRGGK N+VLTFGMNDWQQCPELGPIGD+LSGSEF VRTSVLII QHNLKGE
Sbjct: 721 GQYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGE 780

Query: 781 LDGLDDDFSIM 792
           LDGLDDDFSIM
Sbjct: 781 LDGLDDDFSIM 790

BLAST of Sed0002074.1 vs. ExPASy TrEMBL
Match: A0A5A7UWQ7 (Cation/H(+) antiporter 28 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00850 PE=4 SV=1)

HSP 1 Score: 1359.0 bits (3516), Expect = 0.0e+00
Identity = 680/791 (85.97%), Postives = 740/791 (93.55%), Query Frame = 0

Query: 1   MARTRVHEPLRALANFNKCTIRLTEIFGRATRFILGFFIMVCLCNGIHYFLKPFSQPQIS 60
           MA+TRVH+P R+LA  + CTIRLTEIFGRATR ILGFF+M+ LCNG+HY L+PFSQP+I+
Sbjct: 1   MAKTRVHQPSRSLA-IDSCTIRLTEIFGRATRSILGFFLMMVLCNGVHYLLRPFSQPRIT 60

Query: 61  SDTIVGLVVGNLIRKQLELPTVKTLRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVA 120
           SDTIVGL VGN +RKQL++ T+KTLRY+VDF M+CYMFVLGLE+DPYVLFKAPTRDAKVA
Sbjct: 61  SDTIVGLAVGNFVRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVLFKAPTRDAKVA 120

Query: 121 YAGMISTLILACSITPFISFSKTKEISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGR 180
           YAGMISTLILACSI PFISF+K+KEISFILSLS VLSSTASPVLTR ITSLKIGKSDIGR
Sbjct: 121 YAGMISTLILACSIIPFISFAKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGR 180

Query: 181 LVIAAGMHSDFISTLIICIGYLFCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINW 240
           LVIAAGMHSDFISTL+IC+GYLFCKCQETRIS++KGFQLG  LLIQ++  AKVSP+F+NW
Sbjct: 181 LVIAAGMHSDFISTLLICVGYLFCKCQETRISVMKGFQLGILLLIQAVLTAKVSPVFMNW 240

Query: 241 VNTENPDGKPMKGPHLVLAVAFMAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVG 300
           VN ENP+GKPMKGPHLVLAVAFMAFLC CPT+FGYNPILSAFLAGTFLPREGRVSRWA+G
Sbjct: 241 VNNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIG 300

Query: 301 KINYLLTTVFYPIFFFWMGVESKLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAIL 360
           KINYLLTTVFYPIFFFWMGVESKL+DFEP QIMTWV LILLFAIAT+GKVVGTVIAGAIL
Sbjct: 301 KINYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVTLILLFAIATIGKVVGTVIAGAIL 360

Query: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHI 420
           GFHWPESVALGLLLTMKGHFHIYLAIAA+TAGK TTSTS+VMVI IFFT+VHAPKVV  I
Sbjct: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSMVMVIVIFFTVVHAPKVVERI 420

Query: 421 IKRARKRTPAHRMALQLLDPSSDLKLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTD 480
           I+RARKRTP HRMALQLLDPSS+LK+LLC+HGPQNT AAINIME+SRG ANPGV+VYVTD
Sbjct: 421 IQRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTD 480

Query: 481 MIELTDEIAATLVQGEGVDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALST 540
           MIELTDEIA+TLVQGEGVDS+TVTHTG+T +R+Q+T+AVQSYVDEN EGITLRRMLALST
Sbjct: 481 MIELTDEIASTLVQGEGVDSVTVTHTGLTQMREQVTSAVQSYVDENGEGITLRRMLALST 540

Query: 541 LNSMAHDICILAEESMGALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILV 600
            NSMA DICILAEE MGALIILPFHK+Q  DGSL+EG   FRYVNRKVL++APCSVGILV
Sbjct: 541 FNSMAQDICILAEELMGALIILPFHKSQHGDGSLSEGQAAFRYVNRKVLRHAPCSVGILV 600

Query: 601 DRGLGTVEKISRLCVSQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAEN 660
           DRGLG+VEKISR  VSQNVAVIFIGGKDDREAL YAGRV+RHPGVKLSVIRFLVDADAEN
Sbjct: 601 DRGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAEN 660

Query: 661 AARRAGTYRVSVAEQEKEMKLDDECFARFYEQHVAGKQVAYIEKHLASSSEMYSTLKSLE 720
           AARRAGTYR+SVAEQE EM+ DDECFA FYE+HVAG  VAY+EKHLASSSE YSTLKSLE
Sbjct: 661 AARRAGTYRISVAEQEDEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLE 720

Query: 721 GQYALIIVGRGGKANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKGE 780
           GQYALIIVGRGGK N+VLTFGMNDWQQCPELGPIGD+LSGSEF VRTSVLII QHNLKGE
Sbjct: 721 GQYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGE 780

Query: 781 LDGLDDDFSIM 792
           LDGLDDDFSIM
Sbjct: 781 LDGLDDDFSIM 790

BLAST of Sed0002074.1 vs. ExPASy TrEMBL
Match: A0A6J1KUM2 (cation/H(+) antiporter 28-like OS=Cucurbita maxima OX=3661 GN=LOC111497762 PE=4 SV=1)

HSP 1 Score: 1338.9 bits (3464), Expect = 0.0e+00
Identity = 684/791 (86.47%), Postives = 725/791 (91.66%), Query Frame = 0

Query: 1   MARTRVHEPLRALANFNKCTIRLTEIFGRATRFILGFFIMVCLCNGIHYFLKPFSQPQIS 60
           MARTRV EP R LA  N C IRL+EIFGRATR ILGFFIM+ LCNG+HY L+PFSQP+IS
Sbjct: 1   MARTRVREPSRLLAG-NTCRIRLSEIFGRATRSILGFFIMMGLCNGVHYLLRPFSQPRIS 60

Query: 61  SDTIVGLVVGNLIRKQLELPTVKTLRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVA 120
           SDTIVGLVVGN IRKQL+L T+KTLRY+VDF MICYMF LGLEMDPY+LFKAPTRDAKVA
Sbjct: 61  SDTIVGLVVGNFIRKQLDLSTLKTLRYIVDFGMICYMFSLGLEMDPYILFKAPTRDAKVA 120

Query: 121 YAGMISTLILACSITPFISFSKTKEISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGR 180
           YAGMISTL+LACSITPFISFSKTKEISFILSLS VLSSTASPVLTR IT LKIGKSDIGR
Sbjct: 121 YAGMISTLVLACSITPFISFSKTKEISFILSLSTVLSSTASPVLTRLITRLKIGKSDIGR 180

Query: 181 LVIAAGMHSDFISTLIICIGYLFCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINW 240
           LVIAAGMHSDFISTLIICIGYLFCKCQE R+S  +GFQLG  LLIQ I  AKVSP+F+NW
Sbjct: 181 LVIAAGMHSDFISTLIICIGYLFCKCQEVRLSTTRGFQLGFVLLIQMIVAAKVSPVFMNW 240

Query: 241 VNTENPDGKPMKGPHLVLAVAFMAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVG 300
           VN ENP+GKPMKGPHLVLAVAFMAFLCSCPT+ GYNPILSAFLAGTFLPREGRVSRWA+G
Sbjct: 241 VNNENPEGKPMKGPHLVLAVAFMAFLCSCPTILGYNPILSAFLAGTFLPREGRVSRWAIG 300

Query: 301 KINYLLTTVFYPIFFFWMGVESKLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAIL 360
           KINYLL+TVFYPIFFFWMG ESK SDFEP QIMTWVRLILLF IATVGKVVGTVIAGAIL
Sbjct: 301 KINYLLSTVFYPIFFFWMGAESKWSDFEPGQIMTWVRLILLFVIATVGKVVGTVIAGAIL 360

Query: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHI 420
           GFHWPESVALGLLLTMKGHFHIYLAIAA+TAGKTTTSTSI MVI IFFTIVHAPKVVAHI
Sbjct: 361 GFHWPESVALGLLLTMKGHFHIYLAIAAKTAGKTTTSTSIGMVIVIFFTIVHAPKVVAHI 420

Query: 421 IKRARKRTPAHRMALQLLDPSSDLKLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTD 480
           IKRARKRTP HRMALQLLDPSS+LK+LLC+HG QNTTAAINIME+SRG A PGV+VY+TD
Sbjct: 421 IKRARKRTPTHRMALQLLDPSSELKILLCIHGLQNTTAAINIMEISRGMAKPGVVVYLTD 480

Query: 481 MIELTDEIAATLVQGEGVDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALST 540
           MIELTDEIAATLVQ EG  ++TVTH  V  +RDQITA VQSYVDEN +GITL RMLALS 
Sbjct: 481 MIELTDEIAATLVQVEGEGTLTVTHAEVRQMRDQITAVVQSYVDENGDGITLGRMLALSA 540

Query: 541 LNSMAHDICILAEESMGALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILV 600
            NSMA DICILAEE MGALIILPFHK+QRADGSL+EG+ GFRYVNRKVL+NAPCSVGILV
Sbjct: 541 FNSMAQDICILAEELMGALIILPFHKSQRADGSLSEGNVGFRYVNRKVLRNAPCSVGILV 600

Query: 601 DRGLGTVEKISRLCVSQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAEN 660
           DRGLGTVEKISR   SQNVAVIFI GKDDREAL YA RV+RHPGVKLSVIRFLVDA AEN
Sbjct: 601 DRGLGTVEKISRSYASQNVAVIFISGKDDREALAYAARVARHPGVKLSVIRFLVDAAAEN 660

Query: 661 AARRAGTYRVSVAEQEKEMKLDDECFARFYEQHVAGKQVAYIEKHLASSSEMYSTLKSLE 720
           AARRAGTYR+SVAEQE+EM+LDDECFA FYE+HVAG  VAY+EKHLASS+E YSTLK LE
Sbjct: 661 AARRAGTYRISVAEQEEEMRLDDECFATFYERHVAGGHVAYVEKHLASSAETYSTLKLLE 720

Query: 721 GQYALIIVGRGGKANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKGE 780
           GQYALIIVGRGGK NTVLTFGMNDWQQCPELGPIGDVLSGSEF VRTSVL+IQQHNLKGE
Sbjct: 721 GQYALIIVGRGGKVNTVLTFGMNDWQQCPELGPIGDVLSGSEFSVRTSVLVIQQHNLKGE 780

Query: 781 LDGLDDDFSIM 792
           LDGLDDDFSIM
Sbjct: 781 LDGLDDDFSIM 790

BLAST of Sed0002074.1 vs. TAIR 10
Match: AT3G52080.1 (cation/hydrogen exchanger 28 )

HSP 1 Score: 803.5 bits (2074), Expect = 1.5e-232
Identity = 413/775 (53.29%), Postives = 551/775 (71.10%), Query Frame = 0

Query: 34  ILGFFIMVCLCNGIHYFLKPFSQPQISSDTIVGLVVGNL--IRKQLELPTVKTLRYMVDF 93
           IL F  +  +   +HY +KP  QP +++D  +GL++GN+   R     P   TL  +++F
Sbjct: 27  ILVFIAIFVVRTLLHYLMKPLGQPYLTTDFAIGLILGNIPRFRGAFSGPYSITLNNIIEF 86

Query: 94  AMICYMFVLGLEMDPYVLFKAPTRDAKVAYAGMISTLILACSITPFISFSKTKEISFILS 153
            MIC+MFV+GLEM+P VL + PT+DA +AY  MI+T +LA   TPF+ ++KT    F L+
Sbjct: 87  GMICHMFVMGLEMNPSVLLRPPTKDAFIAYTSMITTFVLAFVTTPFLHYTKTSPYIFSLA 146

Query: 154 LSIVLSSTASPVLTRSITSLKIGKSDIGRLVIAAGMHSDFISTLIICIGYLFCKCQETRI 213
           LS++ SST SP+LTR I +LKI KSD+G+L  AAG+H+D ISTL+ C G++F   ++   
Sbjct: 147 LSLMASSTGSPILTRVIANLKIRKSDLGKLASAAGVHTDMISTLLYCFGFIFFPTEKPLA 206

Query: 214 ----SIIKGFQLGAALLIQSIFVAKVSPIFINWVNTENPDGKPMKGPHLVLAVAFMAFLC 273
                  +   +    L Q  F + VSPIF+NWVN ENP+GKP+KG HLV+++AF+  +C
Sbjct: 207 RPLHRFFRALLMFCLFLAQVTFTSIVSPIFLNWVNNENPEGKPLKGSHLVMSLAFVVLIC 266

Query: 274 SCPTM---FGYNPILSAFLAGTFLPREGRVSRWAVGKINYLLTTVFYPIFFFWMGVESKL 333
           S PT      YNPILSAF AG FLP +GR+S+W + KINYLL+TVFYPIFFFW+G    +
Sbjct: 267 SFPTWPPESMYNPILSAFTAGLFLPNKGRMSKWIINKINYLLSTVFYPIFFFWVGFIIHM 326

Query: 334 SDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAILGFHWPESVALGLLLTMKGHFHIYL 393
            +F+    M WVR   L      GKV GTV+ G +LG+H PE+ +LGLLLT KGHFH+YL
Sbjct: 327 RNFDITDKMAWVRFFSLLGTVIAGKVTGTVLCGLLLGYHVPETASLGLLLTTKGHFHVYL 386

Query: 394 AIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHIIKRARKRTPAHRMALQLLDPSSDL 453
           A  A    +  ++T  +++  I FT+V++P VV  IIKRARKR P H MALQ LDP+++L
Sbjct: 387 AALAIRTNRVKSTTGALIIFIIVFTVVYSPFVVMDIIKRARKRVPVHIMALQWLDPTTEL 446

Query: 454 KLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTDMIELTDEIAATLVQGEGV----DS 513
           ++L+ LHGP N  + +N+ME+  G   PG I Y TDM+ELTDEIAATL +G G     DS
Sbjct: 447 RILIGLHGPHNIGSTLNVMEICHGGREPGSIFYATDMVELTDEIAATLKKGGGAGQSNDS 506

Query: 514 MTVTHTGVTLIRDQITAAVQSYVD-ENREGITLRRMLALSTLNSMAHDICILAEESMGAL 573
           +TVT   VT +R+ ITAAV  Y +  N +G+T+RRMLALST  +MAHD+C LA+E M ++
Sbjct: 507 VTVTDRSVTEMRESITAAVNGYGELRNGQGVTVRRMLALSTFVTMAHDVCGLADELMVSI 566

Query: 574 IILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILVDRGLGTVEKISRLCVSQNV 633
           IILPFHK    DG+L+ GH GFR+VNRK+LKNAPCSVGILVDR  G  E+  R   S  +
Sbjct: 567 IILPFHKRLNPDGTLDAGHAGFRHVNRKILKNAPCSVGILVDRSFGQTEEAWRPGASMGI 626

Query: 634 AVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAENAARRAGTY-RVSVAEQEKE 693
           A+IFIGG+DDREAL +A +V+RHP VKL VIRFL D  ++NA +R+    R SV +QE+E
Sbjct: 627 AIIFIGGRDDREALAFAAQVARHPAVKLKVIRFLEDKSSQNAQKRSSILNRASVVDQEEE 686

Query: 694 MKLDDECFARFYEQHVA-GKQVAYIEKHLASSSEMYSTLKSLEGQYALIIVGR-GGKANT 753
           MKLDDECFA FYE+++A G +V+Y+EKHL +SSE ++ LKSL+G+Y L+IVGR GG+A++
Sbjct: 687 MKLDDECFAEFYERYIAGGGRVSYMEKHLTNSSETFTALKSLDGEYGLVIVGRGGGRASS 746

Query: 754 VLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKGELDGLDDDFSIM 792
            LT G+NDWQQCPELGPIGDVLSGS+F   TS+LIIQQ   +G+L+GL DDF+I+
Sbjct: 747 GLTTGLNDWQQCPELGPIGDVLSGSDFSHNTSMLIIQQQRTRGQLEGLHDDFTIL 801

BLAST of Sed0002074.1 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 364.4 bits (934), Expect = 2.4e-100
Identity = 228/758 (30.08%), Postives = 401/758 (52.90%), Query Frame = 0

Query: 33  FILGFFIMVCLCNGIHYFLKPFSQPQISSDTIVGLVVG-NLIRKQLELP-------TVKT 92
           F+L   ++V +     + LKPF QP++ S+ + G+V+G +++ +  +         +V  
Sbjct: 40  FVLQLTLVVVVTRFFVFILKPFRQPRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMV 99

Query: 93  LRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVAYAGMISTLIL--ACSITPFISFSK 152
           L  M +  ++ ++F++G+EMD  V+ K   R   +A  GM+   ++  A S +   S   
Sbjct: 100 LETMANVGLLYFLFLVGVEMDIMVVRKTGKRALTIAIGGMVLPFLIGAAFSFSMHRSEDH 159

Query: 153 TKEISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGRLVIAAGMHSDFISTLIICIGYL 212
             + ++IL L + LS TA PVL R +  LK+  ++IGR+ ++A + +D  + +++ +   
Sbjct: 160 LGQGTYILFLGVALSVTAFPVLARILAELKLINTEIGRISMSAALVNDMFAWILLALAIA 219

Query: 213 FCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINWVNTENPDGKPMKGPHLVLAVAF 272
             +  +T  + +      A  +   +FV +     I W+  + P+G+     H+ L +  
Sbjct: 220 LAESDKTSFASLWVMISSAVFIAVCVFVVRPG---IAWIIRKTPEGENFSEFHICLILTG 279

Query: 273 MAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVGKINYLLTTVFYPIFFFWMGVES 332
           +          G + +  AF+ G  +P  G +    + K+   ++ +  P+FF   G+++
Sbjct: 280 VMISGFITDAIGTHSVFGAFVFGLVIP-NGPLGLTLIEKLEDFVSGLLLPLFFAISGLKT 339

Query: 333 KLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAILGFHWPESVALGLLLTMKGHFHI 392
            ++  +     TW+ L L+  +A  GKV+GTVI     G    E + LGLLL  KG   +
Sbjct: 340 NIAAIQGP--ATWLTLFLVIFLACAGKVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEM 399

Query: 393 YLAIAAQTAGKTTTSTSIVMV-IAIFFTIVHAPKVVAHIIKRARKRTPAHRMALQLLDPS 452
            +    +        T   MV +A+  T V  P +V  + K  +K     R  +Q   P 
Sbjct: 400 IVLNVGKDQKVLDDETFATMVLVALVMTGVITP-IVTILYKPVKKSVSYKRRTIQQTKPD 459

Query: 453 SDLKLLLCLHGPQNTTAAINIMELSRGRANPGVIVYVTDMIELTDEIAATLVQGEGVDSM 512
           S+L++L+C+H P+N    IN++E S       + +YV  ++ELT   +A L+      S 
Sbjct: 460 SELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIYVLHLVELTGRASAMLIVHNTRKSG 519

Query: 513 TVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALSTLNSMAHDICILAEESMGALII 572
                      D I  A ++Y +++   + ++ + A+S  ++M  D+C LAE+   + II
Sbjct: 520 RPALNRTQAQSDHIINAFENY-EQHAAFVAVQPLTAISPYSTMHEDVCSLAEDKRVSFII 579

Query: 573 LPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILVDRGLGTVEKISRLCVSQNVAV 632
           +PFHK Q  DG +   +  +R VN+ +L+N+PCSVGILVDRGL    +++   VS  VAV
Sbjct: 580 IPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVGILVDRGLNGATRLNSNTVSLQVAV 639

Query: 633 IFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDAD-AENAARRA---GTYRVSVAEQEK 692
           +F GG DDREAL YA R+++HPG+ L+V+RF+ D D A+ A+ RA      ++   +  K
Sbjct: 640 LFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDEDEADTASTRATNDSDLKIPKMDHRK 699

Query: 693 EMKLDDECFARFYEQHVAGKQVAYIEKHLASSSEMYSTLKSLEGQYALIIVGRGGKANTV 752
           + +LDD+    F  ++   + + YIEK +++  E  + ++S++  + L IVGRG   ++ 
Sbjct: 700 QRQLDDDYINLFRAENAEYESIVYIEKLVSNGEETVAAVRSMDSSHDLFIVGRGEGMSSP 759

Query: 753 LTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQH 776
           LT G+ DW +CPELG IGD+L+ S+F    SVL++QQ+
Sbjct: 760 LTAGLTDWSECPELGAIGDLLASSDFAATVSVLVVQQY 789

BLAST of Sed0002074.1 vs. TAIR 10
Match: AT5G41610.1 (cation/H+ exchanger 18 )

HSP 1 Score: 317.8 bits (813), Expect = 2.5e-86
Identity = 227/771 (29.44%), Postives = 402/771 (52.14%), Query Frame = 0

Query: 34  ILGFFIMVCLCNGIHYFLKPFSQPQISSDTIVGLVVGN--LIRKQLELPTV------KTL 93
           IL   I++ L   + Y L+P  QP++ ++ I G+++G   L R +  L  V        L
Sbjct: 35  ILQIVIVIVLTRVLAYLLRPLRQPRVIAEVIGGIMLGPSLLGRSKAFLDAVFPKKSLTVL 94

Query: 94  RYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVAYAGMISTLILACSITPFISFSKTKE 153
             + +  ++ ++F+ GLE+D   L +   +   +A AG+     L    +  +  + +K 
Sbjct: 95  ETLANLGLLFFLFLAGLEIDTKALRRTGKKALGIALAGITLPFALGIGSSFVLKATISKG 154

Query: 154 I---SFILSLSIVLSSTASPVLTRSITSLKIGKSDIGRLVIAAGMHSDFISTLIICIGYL 213
           +   +F++ + + LS TA PVL R +  LK+  ++IGRL ++A   +D  + +++ +   
Sbjct: 155 VNSTAFLVFMGVALSITAFPVLARILAELKLLTTEIGRLAMSAAAVNDVAAWILLALAIA 214

Query: 214 FCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINWVNTENPDGKPMKGPHLVLAVAF 273
                 + +  +  F  G A +I + F+  + PIF  W++    +G+P++  ++   +A 
Sbjct: 215 LSGSNTSPLVSLWVFLSGCAFVIGASFI--IPPIF-RWISRRCHEGEPIEETYICATLAV 274

Query: 274 MAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVGKINYLLTTVFYPIFFFWMGVES 333
           +          G + +  AF+ G  +P+EG  +   V K+  L++ +F P++F   G+++
Sbjct: 275 VLVCGFITDAIGIHSMFGAFVVGVLIPKEGPFAGALVEKVEDLVSGLFLPLYFVASGLKT 334

Query: 334 KLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAILGFHWP--ESVALGLLLTMKGHF 393
            ++  +  Q  +W  L+L+ A A  GK++GT+  G  L F  P  E++ LG L+  KG  
Sbjct: 335 NVATIQGAQ--SWGLLVLVTATACFGKILGTL--GVSLAFKIPMREAITLGFLMNTKGLV 394

Query: 394 H-IYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVA--HIIKRARKRTPAHRMALQL 453
             I L I          + +I++++A+F T +  P V+A     +RA+K       A++ 
Sbjct: 395 ELIVLNIGKDRKVLNDQTFAIMVLMALFTTFITTPVVMAVYKPARRAKKEGEYKHRAVER 454

Query: 454 LDPSSDLKLLLCLHGPQNTTAAINIMELSRG-RANPGVIVYVTDMIELTDEIAATL---- 513
            + ++ L++L C HG  +  + IN++E SRG     G+ VY   + EL++  +A L    
Sbjct: 455 ENTNTQLRILTCFHGAGSIPSMINLLEASRGIEKGEGLCVYALHLRELSERSSAILMVHK 514

Query: 514 VQGEGVDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALSTLNSMAHDICILA 573
           V+  G+        GV    DQ+  A Q++   +R  + +R M A+S+++ +  DIC  A
Sbjct: 515 VRKNGMPFW--NRRGVNADADQVVVAFQAFQQLSR--VNVRPMTAISSMSDIHEDICTTA 574

Query: 574 EESMGALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILVDRGLGTVEKISR 633
                A++ILPFHK Q+ DGSL      +R+VNR+VL  APCSVGI VDRGLG   ++S 
Sbjct: 575 VRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRVLLQAPCSVGIFVDRGLGGSSQVSA 634

Query: 634 LCVSQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAENAARRAGTYRVSV 693
             VS +V V+F GG DDREAL Y  R++ HPG+ L+V RF+V  +             + 
Sbjct: 635 QDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVLTVFRFVVSPERVGEIVNVEVSNNNN 694

Query: 694 AEQE-KEMKLDDECFARFYEQHVAGKQVAYIEKHLASSS-EMYSTLKSLEGQYALIIVGR 753
             Q  K +K D+E  +   +     + V ++EK + +++ ++ S ++ +  +  L +VGR
Sbjct: 695 ENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQIENAAVDVRSAIEEVR-RSNLFLVGR 754

Query: 754 --GGKANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKG 780
             GG+    +   + +  +CPELGP+G +L   E   + SVL+IQQ+N  G
Sbjct: 755 MPGGE----IALAIRENSECPELGPVGSLLISPESSTKASVLVIQQYNGTG 789

BLAST of Sed0002074.1 vs. TAIR 10
Match: AT3G17630.1 (cation/H+ exchanger 19 )

HSP 1 Score: 307.8 bits (787), Expect = 2.6e-83
Identity = 222/757 (29.33%), Postives = 387/757 (51.12%), Query Frame = 0

Query: 34  ILGFFIMVCLCNGIHYFLKPFSQPQISSDTIVGLVVG--NLIRKQLELPTV------KTL 93
           IL   ++V     + YFLKP  QP++ ++ I G+++G   L R +  L T+        L
Sbjct: 36  ILQIVLVVVFTRLLAYFLKPLKQPRVIAEIIGGILLGPSALGRSKAYLDTIFPKKSLTVL 95

Query: 94  RYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVAYAGMISTLILACSITPFISFSKTK- 153
             + +  ++ ++F++GLE+D   + K   +   +A AG+    I+    +  +S + +K 
Sbjct: 96  DTLANIGLLFFLFLVGLELDFAAIKKTGKKSLLIAIAGISLPFIVGVGTSFVLSATISKG 155

Query: 154 --EISFILSLSIVLSSTASPVLTRSITSLKIGKSDIGRLVIAAGMHSDFISTLIICIGYL 213
             ++ FI+ + + LS TA PVL R +  LK+  +DIGR+ ++A   +D  + +++ +   
Sbjct: 156 VDQLPFIVFMGVALSITAFPVLARILAELKLLTTDIGRMAMSAAGVNDVAAWILLALAIA 215

Query: 214 FCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINWVNTENPDGKPMKGPHLVLAVAF 273
                 + +  +     G   +I ++   K     + ++    P+G+P+K  ++ + +  
Sbjct: 216 LSGDGTSPLVSVWVLLCGTGFVIFAVVAIKP---LLAYMARRCPEGEPVKELYVCVTLTV 275

Query: 274 MAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVGKINYLLTTVFYPIFFFWMGVES 333
           +          G + +  AF+ G   P+EG   R    KI  L++ +  P++F   G+++
Sbjct: 276 VLAASFVTDTIGIHALFGAFVVGIVAPKEGPFCRILTEKIEDLVSGLLLPLYFAASGLKT 335

Query: 334 KLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAILGFHWPESVALGLLLTMKGHFH- 393
            ++     Q  +W  L+L+      GK+VGTV +  +    + E+V LG L+  KG    
Sbjct: 336 DVTTIRGAQ--SWGLLVLVILTTCFGKIVGTVGSSMLCKVPFREAVTLGFLMNTKGLVEL 395

Query: 394 IYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVAHIIKRARKRTPAHRMALQLLDPS 453
           I L I          + +I++++A+F T +  P +V  I K ARK  P     +Q  D  
Sbjct: 396 IVLNIGKDRKVLNDQAFAILVLMALFTTFITTP-IVMLIYKPARKGAPYKHRTIQRKDHD 455

Query: 454 SDLKLLLCLHGPQNTTAAINIMELSRGRANPG-VIVYVTDMIELTDEIAATLVQGEGVDS 513
           S+L++L C H  +N    IN++E SRG    G + VY   ++EL++  +A  +  +  ++
Sbjct: 456 SELRILACFHSTRNIPTLINLIESSRGTGKKGRLCVYAMHLMELSERSSAIAMVHKARNN 515

Query: 514 MTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALSTLNSMAHDICILAEESMGALI 573
                  +    DQ+  A ++Y  ++   + +R M A+S L+S+  DIC  A +   A+I
Sbjct: 516 GLPIWNKIERSTDQMVIAFEAY--QHLRAVAVRPMTAISGLSSIHEDICTSAHQKRVAMI 575

Query: 574 ILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILVDRGLGTVEKISRLCVSQNVA 633
           +LPFHK QR DG++      F  VN++VL+ APCSVGILVDRGLG   ++    V+  V 
Sbjct: 576 LLPFHKHQRMDGAMESIGHRFHEVNQRVLQRAPCSVGILVDRGLGGTSQVVASEVAYKVV 635

Query: 634 VIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAENAARRAGTYRVSVAEQEKEMK 693
           + F GG DDREAL Y  ++  HPG+ L+V +F+    A    +R    +    E+EK+ K
Sbjct: 636 IPFFGGLDDREALAYGMKMVEHPGITLTVYKFVA---ARGTLKR--FEKSEHDEKEKKEK 695

Query: 694 LDDECFARFYEQHVAGKQ-VAYIEKHLASSSEMYSTLKSLEGQYALIIVGRGGKANTVLT 753
             DE F R       G + +AY E+ + S  ++ +TLKS+  +  L +VGR     +++ 
Sbjct: 696 ETDEEFVRELMNDPRGNESLAYEERVVESKDDIIATLKSM-SKCNLFVVGRNAAVASLVK 755

Query: 754 FGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHN 777
                   CPELGP+G +LS SEF    SVL++Q ++
Sbjct: 756 S-----TDCPELGPVGRLLSSSEFSTTASVLVVQGYD 773

BLAST of Sed0002074.1 vs. TAIR 10
Match: AT5G41610.2 (cation/H+ exchanger 18 )

HSP 1 Score: 302.0 bits (772), Expect = 1.4e-81
Identity = 211/716 (29.47%), Postives = 376/716 (52.51%), Query Frame = 0

Query: 81  TVKTLRYMVDFAMICYMFVLGLEMDPYVLFKAPTRDAKVAYAGMISTLILACSITPFISF 140
           ++  L  + +  ++ ++F+ GLE+D   L +   +   +A AG+     L    +  +  
Sbjct: 22  SLTVLETLANLGLLFFLFLAGLEIDTKALRRTGKKALGIALAGITLPFALGIGSSFVLKA 81

Query: 141 SKTKEI---SFILSLSIVLSSTASPVLTRSITSLKIGKSDIGRLVIAAGMHSDFISTLII 200
           + +K +   +F++ + + LS TA PVL R +  LK+  ++IGRL ++A   +D  + +++
Sbjct: 82  TISKGVNSTAFLVFMGVALSITAFPVLARILAELKLLTTEIGRLAMSAAAVNDVAAWILL 141

Query: 201 CIGYLFCKCQETRISIIKGFQLGAALLIQSIFVAKVSPIFINWVNTENPDGKPMKGPHLV 260
            +         + +  +  F  G A +I + F+  + PIF  W++    +G+P++  ++ 
Sbjct: 142 ALAIALSGSNTSPLVSLWVFLSGCAFVIGASFI--IPPIF-RWISRRCHEGEPIEETYIC 201

Query: 261 LAVAFMAFLCSCPTMFGYNPILSAFLAGTFLPREGRVSRWAVGKINYLLTTVFYPIFFFW 320
             +A +          G + +  AF+ G  +P+EG  +   V K+  L++ +F P++F  
Sbjct: 202 ATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPKEGPFAGALVEKVEDLVSGLFLPLYFVA 261

Query: 321 MGVESKLSDFEPEQIMTWVRLILLFAIATVGKVVGTVIAGAILGFHWP--ESVALGLLLT 380
            G+++ ++  +  Q  +W  L+L+ A A  GK++GT+  G  L F  P  E++ LG L+ 
Sbjct: 262 SGLKTNVATIQGAQ--SWGLLVLVTATACFGKILGTL--GVSLAFKIPMREAITLGFLMN 321

Query: 381 MKGHFH-IYLAIAAQTAGKTTTSTSIVMVIAIFFTIVHAPKVVA--HIIKRARKRTPAHR 440
            KG    I L I          + +I++++A+F T +  P V+A     +RA+K      
Sbjct: 322 TKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFITTPVVMAVYKPARRAKKEGEYKH 381

Query: 441 MALQLLDPSSDLKLLLCLHGPQNTTAAINIMELSRG-RANPGVIVYVTDMIELTDEIAAT 500
            A++  + ++ L++L C HG  +  + IN++E SRG     G+ VY   + EL++  +A 
Sbjct: 382 RAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGIEKGEGLCVYALHLRELSERSSAI 441

Query: 501 L----VQGEGVDSMTVTHTGVTLIRDQITAAVQSYVDENREGITLRRMLALSTLNSMAHD 560
           L    V+  G+        GV    DQ+  A Q++   +R  + +R M A+S+++ +  D
Sbjct: 442 LMVHKVRKNGMPFW--NRRGVNADADQVVVAFQAFQQLSR--VNVRPMTAISSMSDIHED 501

Query: 561 ICILAEESMGALIILPFHKTQRADGSLNEGHTGFRYVNRKVLKNAPCSVGILVDRGLGTV 620
           IC  A     A++ILPFHK Q+ DGSL      +R+VNR+VL  APCSVGI VDRGLG  
Sbjct: 502 ICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRVLLQAPCSVGIFVDRGLGGS 561

Query: 621 EKISRLCVSQNVAVIFIGGKDDREALVYAGRVSRHPGVKLSVIRFLVDADAENAARRAGT 680
            ++S   VS +V V+F GG DDREAL Y  R++ HPG+ L+V RF+V  +          
Sbjct: 562 SQVSAQDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVLTVFRFVVSPERVGEIVNVEV 621

Query: 681 YRVSVAEQE-KEMKLDDECFARFYEQHVAGKQVAYIEKHLASSS-EMYSTLKSLEGQYAL 740
              +   Q  K +K D+E  +   +     + V ++EK + +++ ++ S ++ +  +  L
Sbjct: 622 SNNNNENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQIENAAVDVRSAIEEVR-RSNL 681

Query: 741 IIVGR--GGKANTVLTFGMNDWQQCPELGPIGDVLSGSEFYVRTSVLIIQQHNLKG 780
            +VGR  GG+    +   + +  +CPELGP+G +L   E   + SVL+IQQ+N  G
Sbjct: 682 FLVGRMPGGE----IALAIRENSECPELGPVGSLLISPESSTKASVLVIQQYNGTG 721