Sed0000633.1 (mRNA) Chayote v1

Overview
NameSed0000633.1
TypemRNA
OrganismSechium edule (Chayote v1)
DescriptionProtein kinase domain-containing protein
LocationLG02: 35602600 .. 35606068 (+)
Sequence length1374
RNA-Seq ExpressionSed0000633.1
SyntenySed0000633.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTCTTGGGCGTTATTTTCCATCTACACGTTTTGAGTGCTAACGCTAAGCTTATGTTGGCTCTTCATAGAAATATTTTCTTGAGTTATTGACAATTTTGATTCATTGAGTTGTATAGTACGGCCATGTCTTTGTAGCAGCATTTTTTGGTCGTGTCAAACCGTGTCGTTTAGAGGCTCTTCGGGGCGGCTAAGGGTTGTAAAAAATGGTTTATATGTGTAGATAAGTGCAATGACTTCTTGTTGCTTTGTAATCCTCAATAGCTGCAAAGGAAACAGCAGCTCTGAGAAACCTGCAGAAGGTGAGAACCAGGTAACTTTGTTTTTTTTTTCAAGATTCTATATTCTATAGTTGTTTTTAAAATGGGGTTCTATAATTTCTGCTATTTTGGTGGTTCCAAGGGAGCCTTGTTGGGTTGAGGCTTTCAAAGCATACATCCTTGGAAGTTCGATAAATGAGCTTTTAATTGTAAAATTCCTTAGGGTGTGTTTGGGGCACCAATTTGGTTATGATAGACGGAGGGTTATGATAAATGTTGGGTTATGATAAGCGGTGTATTATTATAACCCGTGTTTGAGGGCAGGTTATTAATAACTGTATTTGAGGGGCTGGTTATTGTAACAAAAATAATATATGTAAGGTTTAGATAAACGGGTAGATTTAAATAAACGGATATGCAGGTTTAGATAACTATCGACTATAATAAACGGTGGGGCAAATATGAGTTAGGTTATTATAACAAACTCGGCCGTTTACATAAACGGGGACTCAAACACACCCTTATTGTCTCATGGATCTTAACTTTGGGATGGGTGCGGGTAACCTCGAGTATAGTGGAGTTCAACTCCGATTTCCATGTTTAAAAAAAAGATATTTTGATATTTTGGTGACCCAAAAAAGATTTTCTATTTCTCTGTATTAGATAATATGAAGTTTCATTCAAAATTAATTAGTCAATATCTATTATAATGATGTTGAAGTCTTTTCACCTTTCCAACGTGAAACAAAGGGTACAAACTATCTCCAACAAACACTCTTGATTACTAATTACTTTGTGCCTTTGTTTTTTTAACAAAATGAAAGGATGAATTGATTTGCCTGAACAGAAATTTGATACAGCCATAGTCTGCAGTTTTTGGTAGTATTGCTTCCTTGTTGATGATGAATTGATTTTTTTGGGTTGTACTTAGGTTTAGGGATTGCTGTTACCAACAATGTACGCATATTTACTTATAATTCACTGAGGTCAGCAACTAGTAATTTTCATCCATCAAACAGAATAGGTGTGGGAGGTTATGGAGCTGTGTATAAAGTAAGACCATTGATGCATTTTAATCTGTAACATGATCCCTGCAGATACATTTTCATAGGATGCATTTTAATATGTCATGATTTGCAATTTCAGGGAGTTTTAAGGGATGGTACCGATGTTGCAGTCAAGTCATTGTCTGCAGGATCAGCCCAAGGGACTCGTGAATTTTTAACCGAGATAAATATGATATCAAATATACGACATCGAAATCTCGTTGAACTGATTGGTTGTTGCGTTGAAGGTACTCATCGAATTTTGGTGTATGAATATCTAGAGAACAACAGCCTTGCTAGTACTTTGCTTGGTAAGTTTTCAGAGCATTTAAGTTGTTTTTGTGTATCCTAATTCAGTATAAATATGTACCATGTTGTATCTCTAAAAGTTCTTATTCTAGCTAAACATATAGTGGCTGTTTGGACCACCAGTTACTAGTTAACCCATAACCCTTCATCCCCAGCTCTAATAACTCTTCAACCTTGTTAAAATAACCCCTCTTTCAGAGGTTACCATAACTCGTGTATAATGCCAACAAAAGTAGAGTTATAGTAACATCTTGTACAACTTAATAATTAAGAGGTTATAAGTAGTAATTCTCACTCTCAAATGTTGTTGAATAAAGAAAAACTCATGTTATAATAATCTGCCTCAAATACATACCATTATAAATTTACAATAATTCACTGTTTATAATAATTCAACCGTTGCTCCAAACAATCCTCTCTTGAAAATCAGAAATTCGAGTATGTTAAAATGTGTTTCTTATTGTTTGTATTATCTTCTGGCGATATCTAGTCTTTATTTAGTAGAAATCATATCGGTTTAGTTAACTATGATCAATATAAGATTATCGCAAGCCAACTATCATTTCAGGCACAAAGAGTAAGCATGTTATTCTGGATTGGCCAAAAAGAGCTAAGATTTGTGTTGGTACAGCTCTTGGTCTTGCATTTCTACATGAGGAAGCTGAACCAACGGTTGTTCATAGAGATATCAAGGCTAGCAATATACTTCTTGACATGAATTTTCATCCTAAAATAGGAGACTTTGGATTGGCTAAGCTTTTCCCAGACAACGTTACTCATGTTAGTACGCGAGTGGCCGGAACAATGTAAGCATACTATATATATTTCATTCTCTTGATATTTGGAACTTTACTTCTAAATTTATGCCGAGAGACCTTTTATGCACACCTTATCTATTTACTGTGATGTTCCGATCGTGGATGACCTTTTATGTTGAGAGATCTTTTGATATTTCATTTGCTTATGCGTACCTTAATTAATTTCATAGGATTGCTATCTGACTCTTTTATATTTAATTGCTAAGGTAATTCGTAGAAAATTAATTTTTAAGTAGGTCGTCCTATTGTCCGATCTTATAACCACTAATCACTGTGGTTCATGAGTCATACGCACCATCAAAGTTCTCCTCCAACCATTTTGTGGGTATGTACAGTGGATATTTGGCCCCTGAATATGCACTTTTAGGACAATTGACAAAGAAAGCAGATGTATACAGTTTTGGTGTGGTGGTGCTGGAAGTAATCAGTAGAACTAGCAGCAGTAAAGCAGCATTCGGAGAACAGATGTCGAGTCTCGTCGAATGGGTACGAACAACGCTTACCAGTGCTTGTAACTAATAAGATATGGTAATGTAAATGAAGTTGATTTGATTCTTGATTTGTGGGAGTTGTAGACATGGAAATTAAGACAAGAAGGACGGGTGGTAGAGCTCATTGATCCGGAACTGATGGAATATCCAAAAGCAGAGGTGATGCGTTTCATAACAGTTGCACTCTTTTGTACACAAGCAGCTGCAAATCAAAGACCAAACATGAAACAAGTTGTGGAAATGCTTTCAAAAGAAGTGCATATTAATGAAAAACTATTGACTGAGCCAGGAGTGTACAAAACTAGGAAGTTTAATGCTTCTGCTGCTGCTAGTTCAAAGGGATCACAATCCTCCCATACAGACCAAAGGTAAGAAAGCTATGAAGTCTTGTGCATCTTCTACTAAATTTAATAGTACATATAGTCTGACCCAATTGATTCCTAGATGATATACTTGGCCACTGCTTCTCTATTCCCCTTGTAATTTCAATATTTTAGTGGATTAACTTCTGCCTGTGTTTATGAATTCTTTTAACTATTTTCTTG

mRNA sequence

ATTTTCTTGGGCGTTATTTTCCATCTACACGTTTTGAGTGCTAACGCTAAGCTTATGTTGGCTCTTCATAGAAATATTTTCTTGAGTTATTGACAATTTTGATTCATTGAGTTGTATAGTACGGCCATGTCTTTGTAGCAGCATTTTTTGGTCGTGTCAAACCGTGTCGTTTAGAGGCTCTTCGGGGCGGCTAAGGGTTGTAAAAAATGGTTTATATGTGTAGATAAGTGCAATGACTTCTTGTTGCTTTGTAATCCTCAATAGCTGCAAAGGAAACAGCAGCTCTGAGAAACCTGCAGAAGGTGAGAACCAGGGAGTTTTAAGGGATGGTACCGATGTTGCAGTCAAGTCATTGTCTGCAGGATCAGCCCAAGGGACTCGTGAATTTTTAACCGAGATAAATATGATATCAAATATACGACATCGAAATCTCGTTGAACTGATTGGTTGTTGCGTTGAAGGTACTCATCGAATTTTGGTGTATGAATATCTAGAGAACAACAGCCTTGCTAGTACTTTGCTTGGCACAAAGAGTAAGCATGTTATTCTGGATTGGCCAAAAAGAGCTAAGATTTGTGTTGGTACAGCTCTTGGTCTTGCATTTCTACATGAGGAAGCTGAACCAACGGTTGTTCATAGAGATATCAAGGCTAGCAATATACTTCTTGACATGAATTTTCATCCTAAAATAGGAGACTTTGGATTGGCTAAGCTTTTCCCAGACAACGTTACTCATGTTAGTACGCGAGTGGCCGGAACAATTGGATATTTGGCCCCTGAATATGCACTTTTAGGACAATTGACAAAGAAAGCAGATGTATACAGTTTTGGTGTGGTGGTGCTGGAAGTAATCAGTAGAACTAGCAGCAGTAAAGCAGCATTCGGAGAACAGATGTCGAGTCTCGTCGAATGGACATGGAAATTAAGACAAGAAGGACGGGTGGTAGAGCTCATTGATCCGGAACTGATGGAATATCCAAAAGCAGAGGTGATGCGTTTCATAACAGTTGCACTCTTTTGTACACAAGCAGCTGCAAATCAAAGACCAAACATGAAACAAGTTGTGGAAATGCTTTCAAAAGAAGTGCATATTAATGAAAAACTATTGACTGAGCCAGGAGTGTACAAAACTAGGAAGTTTAATGCTTCTGCTGCTGCTAGTTCAAAGGGATCACAATCCTCCCATACAGACCAAAGGTAAGAAAGCTATGAAGTCTTGTGCATCTTCTACTAAATTTAATAGTACATATAGTCTGACCCAATTGATTCCTAGATGATATACTTGGCCACTGCTTCTCTATTCCCCTTGTAATTTCAATATTTTAGTGGATTAACTTCTGCCTGTGTTTATGAATTCTTTTAACTATTTTCTTG

Coding sequence (CDS)

ATGACTTCTTGTTGCTTTGTAATCCTCAATAGCTGCAAAGGAAACAGCAGCTCTGAGAAACCTGCAGAAGGTGAGAACCAGGGAGTTTTAAGGGATGGTACCGATGTTGCAGTCAAGTCATTGTCTGCAGGATCAGCCCAAGGGACTCGTGAATTTTTAACCGAGATAAATATGATATCAAATATACGACATCGAAATCTCGTTGAACTGATTGGTTGTTGCGTTGAAGGTACTCATCGAATTTTGGTGTATGAATATCTAGAGAACAACAGCCTTGCTAGTACTTTGCTTGGCACAAAGAGTAAGCATGTTATTCTGGATTGGCCAAAAAGAGCTAAGATTTGTGTTGGTACAGCTCTTGGTCTTGCATTTCTACATGAGGAAGCTGAACCAACGGTTGTTCATAGAGATATCAAGGCTAGCAATATACTTCTTGACATGAATTTTCATCCTAAAATAGGAGACTTTGGATTGGCTAAGCTTTTCCCAGACAACGTTACTCATGTTAGTACGCGAGTGGCCGGAACAATTGGATATTTGGCCCCTGAATATGCACTTTTAGGACAATTGACAAAGAAAGCAGATGTATACAGTTTTGGTGTGGTGGTGCTGGAAGTAATCAGTAGAACTAGCAGCAGTAAAGCAGCATTCGGAGAACAGATGTCGAGTCTCGTCGAATGGACATGGAAATTAAGACAAGAAGGACGGGTGGTAGAGCTCATTGATCCGGAACTGATGGAATATCCAAAAGCAGAGGTGATGCGTTTCATAACAGTTGCACTCTTTTGTACACAAGCAGCTGCAAATCAAAGACCAAACATGAAACAAGTTGTGGAAATGCTTTCAAAAGAAGTGCATATTAATGAAAAACTATTGACTGAGCCAGGAGTGTACAAAACTAGGAAGTTTAATGCTTCTGCTGCTGCTAGTTCAAAGGGATCACAATCCTCCCATACAGACCAAAGGTAA

Protein sequence

MTSCCFVILNSCKGNSSSEKPAEGENQGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYEYLENNSLASTLLGTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNILLDMNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVLEVISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPELMEYPKAEVMRFITVALFCTQAAANQRPNMKQVVEMLSKEVHINEKLLTEPGVYKTRKFNASAAASSKGSQSSHTDQR
Homology
BLAST of Sed0000633.1 vs. NCBI nr
Match: XP_022145592.1 (cold-responsive protein kinase 1 isoform X2 [Momordica charantia])

HSP 1 Score: 553.1 bits (1424), Expect = 1.5e-153
Identity = 280/324 (86.42%), Postives = 302/324 (93.21%), Query Frame = 0

Query: 1   MTSCCFVILNSCKGNSSSEKPAEGENQGVLRDGTDVAVKSLSAGSAQGTREFLTEINMIS 60
           MTSCCF ILN CKGNSSSE+PAEGENQGVLRDGT VAVKSLSA SAQGTREFLTEINMIS
Sbjct: 7   MTSCCFAILNCCKGNSSSEEPAEGENQGVLRDGTHVAVKSLSAESAQGTREFLTEINMIS 66

Query: 61  NIRHRNLVELIGCCVEGTHRILVYEYLENNSLASTLLGTKSKHVILDWPKRAKICVGTAL 120
           NIRH NLVELIGCC+EG+HRILVYEYLENNSLASTLLGTKSKHVILDWPKRA+IC+GTAL
Sbjct: 67  NIRHHNLVELIGCCIEGSHRILVYEYLENNSLASTLLGTKSKHVILDWPKRAQICLGTAL 126

Query: 121 GLAFLHEEAEPTVVHRDIKASNILLDMNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYL 180
           GLAFLHEEAE +VVHRDIKASNILLD NFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYL
Sbjct: 127 GLAFLHEEAELSVVHRDIKASNILLDRNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYL 186

Query: 181 APEYALLGQLTKKADVYSFGVVVLEVISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVEL 240
           APEYALLGQLTKKADVYSFGV++LEVIS +SSSK AFGE++  LVEWTWKL++EGRV EL
Sbjct: 187 APEYALLGQLTKKADVYSFGVLMLEVISGSSSSKTAFGEELLILVEWTWKLKEEGRVAEL 246

Query: 241 IDPELMEYPKAEVMRFITVALFCTQAAANQRPNMKQVVEMLSKEVHINEKLLTEPGVYK- 300
           IDPEL++YPKAEVMRFI VALFCTQAAANQRP MKQVVEMLS+EVH+NEKLLT+PGVYK 
Sbjct: 247 IDPELIDYPKAEVMRFIIVALFCTQAAANQRPTMKQVVEMLSREVHLNEKLLTKPGVYKG 306

Query: 301 --TRKFNASAAASSKGSQSSHTDQ 322
             +RK N  AA SS+G+ SSHTD+
Sbjct: 307 HRSRKLN-GAAISSEGTSSSHTDK 329

BLAST of Sed0000633.1 vs. NCBI nr
Match: XP_023527443.1 (cold-responsive protein kinase 1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 546.2 bits (1406), Expect = 1.9e-151
Identity = 276/323 (85.45%), Postives = 300/323 (92.88%), Query Frame = 0

Query: 1   MTSCCFVILNSCKGNSSSEKPAEGENQGVLRDGTDVAVKSLSAGSAQGTREFLTEINMIS 60
           M SCCF ILN CKGNSSSE+PAEGENQGVLRDGT+VAVKSLSA S QGTREFLTEINMIS
Sbjct: 1   MASCCFAILNCCKGNSSSEEPAEGENQGVLRDGTNVAVKSLSAESTQGTREFLTEINMIS 60

Query: 61  NIRHRNLVELIGCCVEGTHRILVYEYLENNSLASTLLGTKSKHVILDWPKRAKICVGTAL 120
           +IRH+NLVELIGCCVEGTHRILVYEYLENNSLA+TLLGT SKHV+LDWP RAKIC+GTAL
Sbjct: 61  DIRHQNLVELIGCCVEGTHRILVYEYLENNSLANTLLGTLSKHVVLDWPMRAKICLGTAL 120

Query: 121 GLAFLHEEAEPTVVHRDIKASNILLDMNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYL 180
           GLAFLHEEAEP+VVHRDIKASNILLD NF PKIGDFGLAKLFPDNVTHVSTRVAGT+GYL
Sbjct: 121 GLAFLHEEAEPSVVHRDIKASNILLDRNFDPKIGDFGLAKLFPDNVTHVSTRVAGTVGYL 180

Query: 181 APEYALLGQLTKKADVYSFGVVVLEVISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVEL 240
           APEYALLGQLTKKADVYSFGV++LEVIS +SS+KAAFGE++  LVEWTWKL+QEGR+VEL
Sbjct: 181 APEYALLGQLTKKADVYSFGVLMLEVISGSSSTKAAFGEELLILVEWTWKLKQEGRLVEL 240

Query: 241 IDPELMEYPKAEVMRFITVALFCTQAAANQRPNMKQVVEMLSKEVHINEKLLTEPGVY-- 300
           IDPEL+ YPKAEV+RFITVALFCTQAAANQRP MKQVVEMLS+EVH+NEKLLTEPGVY  
Sbjct: 241 IDPELINYPKAEVVRFITVALFCTQAAANQRPTMKQVVEMLSREVHLNEKLLTEPGVYKG 300

Query: 301 -KTRKFNASAAAS--SKGSQSSH 319
            KTRK N +AAA+  S G+ SSH
Sbjct: 301 HKTRKLNGAAAAASISGGTSSSH 323

BLAST of Sed0000633.1 vs. NCBI nr
Match: XP_022983244.1 (cold-responsive protein kinase 1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 544.7 bits (1402), Expect = 5.5e-151
Identity = 276/323 (85.45%), Postives = 298/323 (92.26%), Query Frame = 0

Query: 1   MTSCCFVILNSCKGNSSSEKPAEGENQGVLRDGTDVAVKSLSAGSAQGTREFLTEINMIS 60
           MTSCCF ILN CKGNSSSE+PAEGENQGVLRDGT+VAVKSLSA S+QGTREFLTEINMIS
Sbjct: 1   MTSCCFAILNCCKGNSSSEEPAEGENQGVLRDGTNVAVKSLSAESSQGTREFLTEINMIS 60

Query: 61  NIRHRNLVELIGCCVEGTHRILVYEYLENNSLASTLLGTKSKHVILDWPKRAKICVGTAL 120
           +IRH+NLVELIGCCVEGTHRILVYEYLENNSLA+TLLGT SKHV+LDWP RAKIC+GTAL
Sbjct: 61  DIRHQNLVELIGCCVEGTHRILVYEYLENNSLANTLLGTLSKHVVLDWPMRAKICLGTAL 120

Query: 121 GLAFLHEEAEPTVVHRDIKASNILLDMNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYL 180
           GLAFLHEEAEP+VVHRDIKASNILLD NF PKIGDFGLAKLFPDNVTHVSTRVAGT+GYL
Sbjct: 121 GLAFLHEEAEPSVVHRDIKASNILLDRNFDPKIGDFGLAKLFPDNVTHVSTRVAGTVGYL 180

Query: 181 APEYALLGQLTKKADVYSFGVVVLEVISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVEL 240
           APEYALLGQLTKKADVYSFGV++LEVIS +SS+KAAFGE++  LVEWTWKL+QEGR+VEL
Sbjct: 181 APEYALLGQLTKKADVYSFGVLMLEVISGSSSTKAAFGEELLILVEWTWKLKQEGRLVEL 240

Query: 241 IDPELMEYPKAEVMRFITVALFCTQAAANQRPNMKQVVEMLSKEVHINEKLLTEPGVY-- 300
           IDPEL+ YPKAEV+RFITVALFCTQAAANQRP MKQVVEMLS+EVH+NE LLTEPGVY  
Sbjct: 241 IDPELINYPKAEVVRFITVALFCTQAAANQRPTMKQVVEMLSREVHLNENLLTEPGVYKG 300

Query: 301 -KTRKFN--ASAAASSKGSQSSH 319
            KTRK N     AA S GS SSH
Sbjct: 301 HKTRKLNGGGGGAAISGGSSSSH 323

BLAST of Sed0000633.1 vs. NCBI nr
Match: XP_022934472.1 (cold-responsive protein kinase 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 540.8 bits (1392), Expect = 8.0e-150
Identity = 274/323 (84.83%), Postives = 298/323 (92.26%), Query Frame = 0

Query: 1   MTSCCFVILNSCKGNSSSEKPAEGENQGVLRDGTDVAVKSLSAGSAQGTREFLTEINMIS 60
           M SCCF ILN CKGNSSSE+PAEGENQGVLRDGT+VAVKSLSA S QGTREFLTEINMIS
Sbjct: 1   MASCCFAILNCCKGNSSSEEPAEGENQGVLRDGTNVAVKSLSAESTQGTREFLTEINMIS 60

Query: 61  NIRHRNLVELIGCCVEGTHRILVYEYLENNSLASTLLGTKSKHVILDWPKRAKICVGTAL 120
           +IRH+NLVELIGCCVEGTHRILVYEYLENNSLA+TLLG+ SK V+LDWP RAKIC+GTAL
Sbjct: 61  DIRHQNLVELIGCCVEGTHRILVYEYLENNSLANTLLGSLSKDVVLDWPMRAKICLGTAL 120

Query: 121 GLAFLHEEAEPTVVHRDIKASNILLDMNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYL 180
           GLAFLHEEAEP+VVHRDIKASNILLD NF PKIGDFGLAKLFPDNVTHVSTRVAGT+GYL
Sbjct: 121 GLAFLHEEAEPSVVHRDIKASNILLDRNFDPKIGDFGLAKLFPDNVTHVSTRVAGTVGYL 180

Query: 181 APEYALLGQLTKKADVYSFGVVVLEVISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVEL 240
           APEYALLGQLTKKADVYSFGV++LEVIS +SS+KAAFGE++  LVEWTWKL+QEGR+VEL
Sbjct: 181 APEYALLGQLTKKADVYSFGVLMLEVISGSSSTKAAFGEELLILVEWTWKLKQEGRLVEL 240

Query: 241 IDPELMEYPKAEVMRFITVALFCTQAAANQRPNMKQVVEMLSKEVHINEKLLTEPGVY-- 300
           IDPEL+ YPKAEV+RFITVALFCTQAAANQRP MKQVVEMLS+EVH+NEKLLTEPGVY  
Sbjct: 241 IDPELINYPKAEVVRFITVALFCTQAAANQRPTMKQVVEMLSREVHLNEKLLTEPGVYKG 300

Query: 301 -KTRKFNASAAAS--SKGSQSSH 319
            KTRK N  AAA+  S G+ SSH
Sbjct: 301 HKTRKLNGGAAAASISGGTSSSH 323

BLAST of Sed0000633.1 vs. NCBI nr
Match: XP_022145585.1 (putative serine/threonine-protein kinase isoform X1 [Momordica charantia] >XP_022145586.1 putative serine/threonine-protein kinase isoform X1 [Momordica charantia] >XP_022145587.1 putative serine/threonine-protein kinase isoform X1 [Momordica charantia] >XP_022145588.1 putative serine/threonine-protein kinase isoform X1 [Momordica charantia] >XP_022145589.1 putative serine/threonine-protein kinase isoform X1 [Momordica charantia] >XP_022145590.1 putative serine/threonine-protein kinase isoform X1 [Momordica charantia] >XP_022145591.1 putative serine/threonine-protein kinase isoform X1 [Momordica charantia])

HSP 1 Score: 528.1 bits (1359), Expect = 5.3e-146
Identity = 277/359 (77.16%), Postives = 300/359 (83.57%), Query Frame = 0

Query: 1   MTSCCFVILNSCKGNSSSEKPAEG-----------------------------------E 60
           MTSCCF ILN CKGNSSSE+PAEG                                    
Sbjct: 7   MTSCCFAILNCCKGNSSSEEPAEGIAVTNNVRIFSYNSLRSATGNFHPSSRIGAGGYGVV 66

Query: 61  NQGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYE 120
            +GVLRDGT VAVKSLSA SAQGTREFLTEINMISNIRH NLVELIGCC+EG+HRILVYE
Sbjct: 67  YKGVLRDGTHVAVKSLSAESAQGTREFLTEINMISNIRHHNLVELIGCCIEGSHRILVYE 126

Query: 121 YLENNSLASTLLGTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNILL 180
           YLENNSLASTLLGTKSKHVILDWPKRA+IC+GTALGLAFLHEEAE +VVHRDIKASNILL
Sbjct: 127 YLENNSLASTLLGTKSKHVILDWPKRAQICLGTALGLAFLHEEAELSVVHRDIKASNILL 186

Query: 181 DMNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVLE 240
           D NFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGV++LE
Sbjct: 187 DRNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVLMLE 246

Query: 241 VISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPELMEYPKAEVMRFITVALFCTQ 300
           VIS +SSSK AFGE++  LVEWTWKL++EGRV ELIDPEL++YPKAEVMRFI VALFCTQ
Sbjct: 247 VISGSSSSKTAFGEELLILVEWTWKLKEEGRVAELIDPELIDYPKAEVMRFIIVALFCTQ 306

Query: 301 AAANQRPNMKQVVEMLSKEVHINEKLLTEPGVYK---TRKFNASAAASSKGSQSSHTDQ 322
           AAANQRP MKQVVEMLS+EVH+NEKLLT+PGVYK   +RK N  AA SS+G+ SSHTD+
Sbjct: 307 AAANQRPTMKQVVEMLSREVHLNEKLLTKPGVYKGHRSRKLN-GAAISSEGTSSSHTDK 364

BLAST of Sed0000633.1 vs. ExPASy Swiss-Prot
Match: C0LGH3 (Probable LRR receptor-like serine/threonine-protein kinase At1g56140 OS=Arabidopsis thaliana OX=3702 GN=At1g56140 PE=2 SV=2)

HSP 1 Score: 295.4 bits (755), Expect = 7.6e-79
Identity = 152/287 (52.96%), Postives = 196/287 (68.29%), Query Frame = 0

Query: 27  QGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYEY 86
           +G L DG +VAVK LS GS QG  +F+ EI  IS ++HRNLV+L GCC EG HR+LVYEY
Sbjct: 709 KGKLNDGREVAVKLLSVGSRQGKGQFVAEIVAISAVQHRNLVKLYGCCYEGEHRLLVYEY 768

Query: 87  LENNSLASTLLGTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNILLD 146
           L N SL   L G K+ H  LDW  R +IC+G A GL +LHEEA   +VHRD+KASNILLD
Sbjct: 769 LPNGSLDQALFGEKTLH--LDWSTRYEICLGVARGLVYLHEEARLRIVHRDVKASNILLD 828

Query: 147 MNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVLEV 206
               PK+ DFGLAKL+ D  TH+STRVAGTIGYLAPEYA+ G LT+K DVY+FGVV LE+
Sbjct: 829 SKLVPKVSDFGLAKLYDDKKTHISTRVAGTIGYLAPEYAMRGHLTEKTDVYAFGVVALEL 888

Query: 207 ISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPELMEYPKAEVMRFITVALFCTQA 266
           +S   +S     ++   L+EW W L ++GR VELID +L E+   E  R I +AL CTQ 
Sbjct: 889 VSGRPNSDENLEDEKRYLLEWAWNLHEKGREVELIDHQLTEFNMEEGKRMIGIALLCTQT 948

Query: 267 AANQRPNMKQVVEMLSKEVHINEKLLTEPGVYKTRKFNASAAASSKG 314
           +   RP M +VV MLS +V +++ + ++PG     +F+ + A+S  G
Sbjct: 949 SHALRPPMSRVVAMLSGDVEVSD-VTSKPGYLTDWRFDDTTASSISG 992

BLAST of Sed0000633.1 vs. ExPASy Swiss-Prot
Match: Q93YN1 (Cold-responsive protein kinase 1 OS=Arabidopsis thaliana OX=3702 GN=CRPK1 PE=1 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.7e-78
Identity = 149/274 (54.38%), Postives = 190/274 (69.34%), Query Frame = 0

Query: 27  QGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYEY 86
           +G L+DG   A+K LSA S QG +EFLTEIN+IS I+H NLV+L GCCVEG HRILVY +
Sbjct: 57  KGCLKDGKLAAIKVLSAESRQGVKEFLTEINVISEIQHENLVKLYGCCVEGNHRILVYNF 116

Query: 87  LENNSLASTLL--GTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNIL 146
           LENNSL  TLL  G     +  DW  RA ICVG A GLAFLHEE  P ++HRDIKASNIL
Sbjct: 117 LENNSLDKTLLAGGYTRSGIQFDWSSRANICVGVAKGLAFLHEEVRPHIIHRDIKASNIL 176

Query: 147 LDMNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVL 206
           LD    PKI DFGLA+L P N+THVSTRVAGTIGYLAPEYA+ GQLT+KAD+YSFGV+++
Sbjct: 177 LDKYLSPKISDFGLARLMPPNMTHVSTRVAGTIGYLAPEYAVRGQLTRKADIYSFGVLLM 236

Query: 207 EVISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPELM-EYPKAEVMRFITVALFC 266
           E++S  S+       +   L+E  W+L +   +V+L+D  L   +   E  R++ + L C
Sbjct: 237 EIVSGRSNKNTRLPTEYQYLLERAWELYERNELVDLVDSGLNGVFDAEEACRYLKIGLLC 296

Query: 267 TQAAANQRPNMKQVVEMLSKEVHINEKLLTEPGV 298
           TQ +   RP+M  VV +L+ E  I+ K ++ PG+
Sbjct: 297 TQDSPKLRPSMSTVVRLLTGEKDIDYKKISRPGL 330

BLAST of Sed0000633.1 vs. ExPASy Swiss-Prot
Match: C0LGH2 (Probable LRR receptor-like serine/threonine-protein kinase At1g56130 OS=Arabidopsis thaliana OX=3702 GN=At1g56130 PE=2 SV=2)

HSP 1 Score: 289.7 bits (740), Expect = 4.2e-77
Identity = 152/293 (51.88%), Postives = 194/293 (66.21%), Query Frame = 0

Query: 27  QGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYEY 86
           +G L DG  VAVK LS GS QG  +F+ EI  IS++ HRNLV+L GCC EG HR+LVYEY
Sbjct: 710 KGNLNDGRVVAVKLLSVGSRQGKGQFVAEIVAISSVLHRNLVKLYGCCFEGEHRMLVYEY 769

Query: 87  LENNSLASTLLGTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNILLD 146
           L N SL   L G K+ H  LDW  R +IC+G A GL +LHEEA   +VHRD+KASNILLD
Sbjct: 770 LPNGSLDQALFGDKTLH--LDWSTRYEICLGVARGLVYLHEEASVRIVHRDVKASNILLD 829

Query: 147 MNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVLEV 206
               P+I DFGLAKL+ D  TH+STRVAGTIGYLAPEYA+ G LT+K DVY+FGVV LE+
Sbjct: 830 SRLVPQISDFGLAKLYDDKKTHISTRVAGTIGYLAPEYAMRGHLTEKTDVYAFGVVALEL 889

Query: 207 ISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPELMEYPKAEVMRFITVALFCTQA 266
           +S   +S     E+   L+EW W L ++ R +ELID +L ++   E  R I +AL CTQ 
Sbjct: 890 VSGRPNSDENLEEEKKYLLEWAWNLHEKSRDIELIDDKLTDFNMEEAKRMIGIALLCTQT 949

Query: 267 AANQRPNMKQVVEMLSKEVHINEKLLTEPGVYKTRKFNASAAASSKGSQSSHT 320
           +   RP M +VV MLS +V I + + ++PG     +F+ +  +S  G Q   T
Sbjct: 950 SHALRPPMSRVVAMLSGDVEIGD-VTSKPGYVSDWRFDDTTGSSLSGFQIKDT 999

BLAST of Sed0000633.1 vs. ExPASy Swiss-Prot
Match: C0LGG8 (Probable LRR receptor-like serine/threonine-protein kinase At1g53430 OS=Arabidopsis thaliana OX=3702 GN=At1g53430 PE=1 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 5.5e-77
Identity = 149/300 (49.67%), Postives = 199/300 (66.33%), Query Frame = 0

Query: 27  QGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYEY 86
           +GVL DG  +AVK LS+ S QG REF+TEI MIS ++H NLV+L GCC+EG   +LVYEY
Sbjct: 685 KGVLADGMTIAVKQLSSKSKQGNREFVTEIGMISALQHPNLVKLYGCCIEGKELLLVYEY 744

Query: 87  LENNSLASTLLGTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNILLD 146
           LENNSLA  L GT+ + + LDW  R KIC+G A GLA+LHEE+   +VHRDIKA+N+LLD
Sbjct: 745 LENNSLARALFGTEKQRLHLDWSTRNKICIGIAKGLAYLHEESRLKIVHRDIKATNVLLD 804

Query: 147 MNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVLEV 206
           ++ + KI DFGLAKL  D  TH+STR+AGTIGY+APEYA+ G LT KADVYSFGVV LE+
Sbjct: 805 LSLNAKISDFGLAKLNDDENTHISTRIAGTIGYMAPEYAMRGYLTDKADVYSFGVVCLEI 864

Query: 207 ISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPEL-MEYPKAEVMRFITVALFCTQ 266
           +S  S++     E+   L++W + L+++G ++EL+DP+L   + K E MR + +AL CT 
Sbjct: 865 VSGKSNTNYRPKEEFVYLLDWAYVLQEQGSLLELVDPDLGTSFSKKEAMRMLNIALLCTN 924

Query: 267 AAANQRPNMKQVVEMLSKEVHINEKLL---TEPGVYKTRKFNASAAASSKGSQSSHTDQR 323
            +   RP M  VV ML  ++ +   L+    +P      +F A    S        T  R
Sbjct: 925 PSPTLRPPMSSVVSMLEGKIKVQPPLVKREADPSGSAAMRFKALELLSQDSESQVSTYAR 984

BLAST of Sed0000633.1 vs. ExPASy Swiss-Prot
Match: C0LGG9 (Probable LRR receptor-like serine/threonine-protein kinase At1g53440 OS=Arabidopsis thaliana OX=3702 GN=At1g53440 PE=2 SV=2)

HSP 1 Score: 287.7 bits (735), Expect = 1.6e-76
Identity = 146/296 (49.32%), Postives = 201/296 (67.91%), Query Frame = 0

Query: 27  QGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYEY 86
           +GVL DG  +AVK LS+ S QG REF+TEI MIS ++H NLV+L GCC+EG   +LVYEY
Sbjct: 683 KGVLADGMTIAVKQLSSKSKQGNREFVTEIGMISALQHPNLVKLYGCCIEGKELLLVYEY 742

Query: 87  LENNSLASTLLGTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNILLD 146
           LENNSLA  L GT+ + + LDW  R K+C+G A GLA+LHEE+   +VHRDIKA+N+LLD
Sbjct: 743 LENNSLARALFGTEKQRLHLDWSTRNKVCIGIAKGLAYLHEESRLKIVHRDIKATNVLLD 802

Query: 147 MNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVLEV 206
           ++ + KI DFGLAKL  +  TH+STR+AGTIGY+APEYA+ G LT KADVYSFGVV LE+
Sbjct: 803 LSLNAKISDFGLAKLDEEENTHISTRIAGTIGYMAPEYAMRGYLTDKADVYSFGVVCLEI 862

Query: 207 ISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPEL-MEYPKAEVMRFITVALFCTQ 266
           +S  S++     E+   L++W + L+++G ++EL+DP+L   + K E MR + +AL CT 
Sbjct: 863 VSGKSNTNYRPKEEFIYLLDWAYVLQEQGSLLELVDPDLGTSFSKKEAMRMLNIALLCTN 922

Query: 267 AAANQRPNMKQVVEMLSKEVHINEKLLTEPGVYKTRKFNASAAASSKGSQSSHTDQ 322
            +   RP M  VV ML  ++ +   L+        R+ + S +A+ +     H  Q
Sbjct: 923 PSPTLRPPMSSVVSMLQGKIKVQPPLV-------KREADPSGSAAMRFKALEHLSQ 971

BLAST of Sed0000633.1 vs. ExPASy TrEMBL
Match: A0A6J1CX22 (cold-responsive protein kinase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015002 PE=4 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 7.5e-154
Identity = 280/324 (86.42%), Postives = 302/324 (93.21%), Query Frame = 0

Query: 1   MTSCCFVILNSCKGNSSSEKPAEGENQGVLRDGTDVAVKSLSAGSAQGTREFLTEINMIS 60
           MTSCCF ILN CKGNSSSE+PAEGENQGVLRDGT VAVKSLSA SAQGTREFLTEINMIS
Sbjct: 7   MTSCCFAILNCCKGNSSSEEPAEGENQGVLRDGTHVAVKSLSAESAQGTREFLTEINMIS 66

Query: 61  NIRHRNLVELIGCCVEGTHRILVYEYLENNSLASTLLGTKSKHVILDWPKRAKICVGTAL 120
           NIRH NLVELIGCC+EG+HRILVYEYLENNSLASTLLGTKSKHVILDWPKRA+IC+GTAL
Sbjct: 67  NIRHHNLVELIGCCIEGSHRILVYEYLENNSLASTLLGTKSKHVILDWPKRAQICLGTAL 126

Query: 121 GLAFLHEEAEPTVVHRDIKASNILLDMNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYL 180
           GLAFLHEEAE +VVHRDIKASNILLD NFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYL
Sbjct: 127 GLAFLHEEAELSVVHRDIKASNILLDRNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYL 186

Query: 181 APEYALLGQLTKKADVYSFGVVVLEVISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVEL 240
           APEYALLGQLTKKADVYSFGV++LEVIS +SSSK AFGE++  LVEWTWKL++EGRV EL
Sbjct: 187 APEYALLGQLTKKADVYSFGVLMLEVISGSSSSKTAFGEELLILVEWTWKLKEEGRVAEL 246

Query: 241 IDPELMEYPKAEVMRFITVALFCTQAAANQRPNMKQVVEMLSKEVHINEKLLTEPGVYK- 300
           IDPEL++YPKAEVMRFI VALFCTQAAANQRP MKQVVEMLS+EVH+NEKLLT+PGVYK 
Sbjct: 247 IDPELIDYPKAEVMRFIIVALFCTQAAANQRPTMKQVVEMLSREVHLNEKLLTKPGVYKG 306

Query: 301 --TRKFNASAAASSKGSQSSHTDQ 322
             +RK N  AA SS+G+ SSHTD+
Sbjct: 307 HRSRKLN-GAAISSEGTSSSHTDK 329

BLAST of Sed0000633.1 vs. ExPASy TrEMBL
Match: A0A6J1J6S5 (cold-responsive protein kinase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481880 PE=4 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 2.7e-151
Identity = 276/323 (85.45%), Postives = 298/323 (92.26%), Query Frame = 0

Query: 1   MTSCCFVILNSCKGNSSSEKPAEGENQGVLRDGTDVAVKSLSAGSAQGTREFLTEINMIS 60
           MTSCCF ILN CKGNSSSE+PAEGENQGVLRDGT+VAVKSLSA S+QGTREFLTEINMIS
Sbjct: 1   MTSCCFAILNCCKGNSSSEEPAEGENQGVLRDGTNVAVKSLSAESSQGTREFLTEINMIS 60

Query: 61  NIRHRNLVELIGCCVEGTHRILVYEYLENNSLASTLLGTKSKHVILDWPKRAKICVGTAL 120
           +IRH+NLVELIGCCVEGTHRILVYEYLENNSLA+TLLGT SKHV+LDWP RAKIC+GTAL
Sbjct: 61  DIRHQNLVELIGCCVEGTHRILVYEYLENNSLANTLLGTLSKHVVLDWPMRAKICLGTAL 120

Query: 121 GLAFLHEEAEPTVVHRDIKASNILLDMNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYL 180
           GLAFLHEEAEP+VVHRDIKASNILLD NF PKIGDFGLAKLFPDNVTHVSTRVAGT+GYL
Sbjct: 121 GLAFLHEEAEPSVVHRDIKASNILLDRNFDPKIGDFGLAKLFPDNVTHVSTRVAGTVGYL 180

Query: 181 APEYALLGQLTKKADVYSFGVVVLEVISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVEL 240
           APEYALLGQLTKKADVYSFGV++LEVIS +SS+KAAFGE++  LVEWTWKL+QEGR+VEL
Sbjct: 181 APEYALLGQLTKKADVYSFGVLMLEVISGSSSTKAAFGEELLILVEWTWKLKQEGRLVEL 240

Query: 241 IDPELMEYPKAEVMRFITVALFCTQAAANQRPNMKQVVEMLSKEVHINEKLLTEPGVY-- 300
           IDPEL+ YPKAEV+RFITVALFCTQAAANQRP MKQVVEMLS+EVH+NE LLTEPGVY  
Sbjct: 241 IDPELINYPKAEVVRFITVALFCTQAAANQRPTMKQVVEMLSREVHLNENLLTEPGVYKG 300

Query: 301 -KTRKFN--ASAAASSKGSQSSH 319
            KTRK N     AA S GS SSH
Sbjct: 301 HKTRKLNGGGGGAAISGGSSSSH 323

BLAST of Sed0000633.1 vs. ExPASy TrEMBL
Match: A0A6J1F2V1 (cold-responsive protein kinase 1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441641 PE=4 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 3.9e-150
Identity = 274/323 (84.83%), Postives = 298/323 (92.26%), Query Frame = 0

Query: 1   MTSCCFVILNSCKGNSSSEKPAEGENQGVLRDGTDVAVKSLSAGSAQGTREFLTEINMIS 60
           M SCCF ILN CKGNSSSE+PAEGENQGVLRDGT+VAVKSLSA S QGTREFLTEINMIS
Sbjct: 1   MASCCFAILNCCKGNSSSEEPAEGENQGVLRDGTNVAVKSLSAESTQGTREFLTEINMIS 60

Query: 61  NIRHRNLVELIGCCVEGTHRILVYEYLENNSLASTLLGTKSKHVILDWPKRAKICVGTAL 120
           +IRH+NLVELIGCCVEGTHRILVYEYLENNSLA+TLLG+ SK V+LDWP RAKIC+GTAL
Sbjct: 61  DIRHQNLVELIGCCVEGTHRILVYEYLENNSLANTLLGSLSKDVVLDWPMRAKICLGTAL 120

Query: 121 GLAFLHEEAEPTVVHRDIKASNILLDMNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYL 180
           GLAFLHEEAEP+VVHRDIKASNILLD NF PKIGDFGLAKLFPDNVTHVSTRVAGT+GYL
Sbjct: 121 GLAFLHEEAEPSVVHRDIKASNILLDRNFDPKIGDFGLAKLFPDNVTHVSTRVAGTVGYL 180

Query: 181 APEYALLGQLTKKADVYSFGVVVLEVISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVEL 240
           APEYALLGQLTKKADVYSFGV++LEVIS +SS+KAAFGE++  LVEWTWKL+QEGR+VEL
Sbjct: 181 APEYALLGQLTKKADVYSFGVLMLEVISGSSSTKAAFGEELLILVEWTWKLKQEGRLVEL 240

Query: 241 IDPELMEYPKAEVMRFITVALFCTQAAANQRPNMKQVVEMLSKEVHINEKLLTEPGVY-- 300
           IDPEL+ YPKAEV+RFITVALFCTQAAANQRP MKQVVEMLS+EVH+NEKLLTEPGVY  
Sbjct: 241 IDPELINYPKAEVVRFITVALFCTQAAANQRPTMKQVVEMLSREVHLNEKLLTEPGVYKG 300

Query: 301 -KTRKFNASAAAS--SKGSQSSH 319
            KTRK N  AAA+  S G+ SSH
Sbjct: 301 HKTRKLNGGAAAASISGGTSSSH 323

BLAST of Sed0000633.1 vs. ExPASy TrEMBL
Match: A0A6J1CWR1 (putative serine/threonine-protein kinase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015002 PE=3 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.6e-146
Identity = 277/359 (77.16%), Postives = 300/359 (83.57%), Query Frame = 0

Query: 1   MTSCCFVILNSCKGNSSSEKPAEG-----------------------------------E 60
           MTSCCF ILN CKGNSSSE+PAEG                                    
Sbjct: 7   MTSCCFAILNCCKGNSSSEEPAEGIAVTNNVRIFSYNSLRSATGNFHPSSRIGAGGYGVV 66

Query: 61  NQGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYE 120
            +GVLRDGT VAVKSLSA SAQGTREFLTEINMISNIRH NLVELIGCC+EG+HRILVYE
Sbjct: 67  YKGVLRDGTHVAVKSLSAESAQGTREFLTEINMISNIRHHNLVELIGCCIEGSHRILVYE 126

Query: 121 YLENNSLASTLLGTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNILL 180
           YLENNSLASTLLGTKSKHVILDWPKRA+IC+GTALGLAFLHEEAE +VVHRDIKASNILL
Sbjct: 127 YLENNSLASTLLGTKSKHVILDWPKRAQICLGTALGLAFLHEEAELSVVHRDIKASNILL 186

Query: 181 DMNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVLE 240
           D NFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGV++LE
Sbjct: 187 DRNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVLMLE 246

Query: 241 VISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPELMEYPKAEVMRFITVALFCTQ 300
           VIS +SSSK AFGE++  LVEWTWKL++EGRV ELIDPEL++YPKAEVMRFI VALFCTQ
Sbjct: 247 VISGSSSSKTAFGEELLILVEWTWKLKEEGRVAELIDPELIDYPKAEVMRFIIVALFCTQ 306

Query: 301 AAANQRPNMKQVVEMLSKEVHINEKLLTEPGVYK---TRKFNASAAASSKGSQSSHTDQ 322
           AAANQRP MKQVVEMLS+EVH+NEKLLT+PGVYK   +RK N  AA SS+G+ SSHTD+
Sbjct: 307 AAANQRPTMKQVVEMLSREVHLNEKLLTKPGVYKGHRSRKLN-GAAISSEGTSSSHTDK 364

BLAST of Sed0000633.1 vs. ExPASy TrEMBL
Match: A0A6J1IYR4 (putative serine/threonine-protein kinase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481880 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 9.2e-144
Identity = 273/358 (76.26%), Postives = 296/358 (82.68%), Query Frame = 0

Query: 1   MTSCCFVILNSCKGNSSSEKPAEG-----------------------------------E 60
           MTSCCF ILN CKGNSSSE+PAEG                                    
Sbjct: 1   MTSCCFAILNCCKGNSSSEEPAEGIAVNNNVRIFSYNSLRSATSNFHPSSRIGAGGYGVV 60

Query: 61  NQGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYE 120
            +GVLRDGT+VAVKSLSA S+QGTREFLTEINMIS+IRH+NLVELIGCCVEGTHRILVYE
Sbjct: 61  YKGVLRDGTNVAVKSLSAESSQGTREFLTEINMISDIRHQNLVELIGCCVEGTHRILVYE 120

Query: 121 YLENNSLASTLLGTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNILL 180
           YLENNSLA+TLLGT SKHV+LDWP RAKIC+GTALGLAFLHEEAEP+VVHRDIKASNILL
Sbjct: 121 YLENNSLANTLLGTLSKHVVLDWPMRAKICLGTALGLAFLHEEAEPSVVHRDIKASNILL 180

Query: 181 DMNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVLE 240
           D NF PKIGDFGLAKLFPDNVTHVSTRVAGT+GYLAPEYALLGQLTKKADVYSFGV++LE
Sbjct: 181 DRNFDPKIGDFGLAKLFPDNVTHVSTRVAGTVGYLAPEYALLGQLTKKADVYSFGVLMLE 240

Query: 241 VISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPELMEYPKAEVMRFITVALFCTQ 300
           VIS +SS+KAAFGE++  LVEWTWKL+QEGR+VELIDPEL+ YPKAEV+RFITVALFCTQ
Sbjct: 241 VISGSSSTKAAFGEELLILVEWTWKLKQEGRLVELIDPELINYPKAEVVRFITVALFCTQ 300

Query: 301 AAANQRPNMKQVVEMLSKEVHINEKLLTEPGVY---KTRKFN--ASAAASSKGSQSSH 319
           AAANQRP MKQVVEMLS+EVH+NE LLTEPGVY   KTRK N     AA S GS SSH
Sbjct: 301 AAANQRPTMKQVVEMLSREVHLNENLLTEPGVYKGHKTRKLNGGGGGAAISGGSSSSH 358

BLAST of Sed0000633.1 vs. TAIR 10
Match: AT3G09010.1 (Protein kinase superfamily protein )

HSP 1 Score: 427.2 bits (1097), Expect = 1.2e-119
Identity = 213/274 (77.74%), Postives = 241/274 (87.96%), Query Frame = 0

Query: 27  QGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYEY 86
           +GVLRDGT VAVKSLSA S QGTREFLTEIN+ISNI H NLV+LIGCC+EG +RILVYEY
Sbjct: 62  KGVLRDGTQVAVKSLSAESKQGTREFLTEINLISNIHHPNLVKLIGCCIEGNNRILVYEY 121

Query: 87  LENNSLASTLLGTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNILLD 146
           LENNSLAS LLG++S++V LDW KRA ICVGTA GLAFLHEE EP VVHRDIKASNILLD
Sbjct: 122 LENNSLASVLLGSRSRYVPLDWSKRAAICVGTASGLAFLHEEVEPHVVHRDIKASNILLD 181

Query: 147 MNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVLEV 206
            NF PKIGDFGLAKLFPDNVTHVSTRVAGT+GYLAPEYALLGQLTKKADVYSFG++VLEV
Sbjct: 182 SNFSPKIGDFGLAKLFPDNVTHVSTRVAGTVGYLAPEYALLGQLTKKADVYSFGILVLEV 241

Query: 207 ISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPELMEYPKAEVMRFITVALFCTQA 266
           IS  SS++AAFG++   LVEW WKLR+E R++E +DPEL ++P  EV RFI VALFCTQA
Sbjct: 242 ISGNSSTRAAFGDEYMVLVEWVWKLREERRLLECVDPELTKFPADEVTRFIKVALFCTQA 301

Query: 267 AANQRPNMKQVVEML-SKEVHINEKLLTEPGVYK 300
           AA +RPNMKQV+EML  KE+++NE  LTEPGVY+
Sbjct: 302 AAQKRPNMKQVMEMLRRKELNLNEDALTEPGVYR 335

BLAST of Sed0000633.1 vs. TAIR 10
Match: AT1G56120.1 (Leucine-rich repeat transmembrane protein kinase )

HSP 1 Score: 297.0 bits (759), Expect = 1.9e-80
Identity = 155/293 (52.90%), Postives = 195/293 (66.55%), Query Frame = 0

Query: 27   QGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYEY 86
            +G L DG +VAVK LS GS QG  +F+ EI  IS++ HRNLV+L GCC EG HR+LVYEY
Sbjct: 726  KGNLNDGREVAVKQLSIGSRQGKGQFVAEIIAISSVLHRNLVKLYGCCFEGDHRLLVYEY 785

Query: 87   LENNSLASTLLGTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNILLD 146
            L N SL   L G KS H  LDW  R +IC+G A GL +LHEEA   ++HRD+KASNILLD
Sbjct: 786  LPNGSLDQALFGDKSLH--LDWSTRYEICLGVARGLVYLHEEASVRIIHRDVKASNILLD 845

Query: 147  MNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVLEV 206
                PK+ DFGLAKL+ D  TH+STRVAGTIGYLAPEYA+ G LT+K DVY+FGVV LE+
Sbjct: 846  SELVPKVSDFGLAKLYDDKKTHISTRVAGTIGYLAPEYAMRGHLTEKTDVYAFGVVALEL 905

Query: 207  ISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPELMEYPKAEVMRFITVALFCTQA 266
            +S   +S     E    L+EW W L ++ R VELID EL EY   EV R I +AL CTQ+
Sbjct: 906  VSGRKNSDENLEEGKKYLLEWAWNLHEKNRDVELIDDELSEYNMEEVKRMIGIALLCTQS 965

Query: 267  AANQRPNMKQVVEMLSKEVHINEKLLTEPGVYKTRKFNASAAASSKGSQSSHT 320
            +   RP M +VV MLS +  +N+   ++PG      F+ + ++S    Q+  T
Sbjct: 966  SYALRPPMSRVVAMLSGDAEVND-ATSKPGYLTDCTFDDTTSSSFSNFQTKDT 1015

BLAST of Sed0000633.1 vs. TAIR 10
Match: AT1G56140.1 (Leucine-rich repeat transmembrane protein kinase )

HSP 1 Score: 295.4 bits (755), Expect = 5.4e-80
Identity = 152/287 (52.96%), Postives = 196/287 (68.29%), Query Frame = 0

Query: 27  QGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYEY 86
           +G L DG +VAVK LS GS QG  +F+ EI  IS ++HRNLV+L GCC EG HR+LVYEY
Sbjct: 709 KGKLNDGREVAVKLLSVGSRQGKGQFVAEIVAISAVQHRNLVKLYGCCYEGEHRLLVYEY 768

Query: 87  LENNSLASTLLGTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNILLD 146
           L N SL   L G K+ H  LDW  R +IC+G A GL +LHEEA   +VHRD+KASNILLD
Sbjct: 769 LPNGSLDQALFGEKTLH--LDWSTRYEICLGVARGLVYLHEEARLRIVHRDVKASNILLD 828

Query: 147 MNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVLEV 206
               PK+ DFGLAKL+ D  TH+STRVAGTIGYLAPEYA+ G LT+K DVY+FGVV LE+
Sbjct: 829 SKLVPKVSDFGLAKLYDDKKTHISTRVAGTIGYLAPEYAMRGHLTEKTDVYAFGVVALEL 888

Query: 207 ISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPELMEYPKAEVMRFITVALFCTQA 266
           +S   +S     ++   L+EW W L ++GR VELID +L E+   E  R I +AL CTQ 
Sbjct: 889 VSGRPNSDENLEDEKRYLLEWAWNLHEKGREVELIDHQLTEFNMEEGKRMIGIALLCTQT 948

Query: 267 AANQRPNMKQVVEMLSKEVHINEKLLTEPGVYKTRKFNASAAASSKG 314
           +   RP M +VV MLS +V +++ + ++PG     +F+ + A+S  G
Sbjct: 949 SHALRPPMSRVVAMLSGDVEVSD-VTSKPGYLTDWRFDDTTASSISG 992

BLAST of Sed0000633.1 vs. TAIR 10
Match: AT1G16670.1 (Protein kinase superfamily protein )

HSP 1 Score: 294.3 bits (752), Expect = 1.2e-79
Identity = 149/274 (54.38%), Postives = 190/274 (69.34%), Query Frame = 0

Query: 27  QGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYEY 86
           +G L+DG   A+K LSA S QG +EFLTEIN+IS I+H NLV+L GCCVEG HRILVY +
Sbjct: 57  KGCLKDGKLAAIKVLSAESRQGVKEFLTEINVISEIQHENLVKLYGCCVEGNHRILVYNF 116

Query: 87  LENNSLASTLL--GTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNIL 146
           LENNSL  TLL  G     +  DW  RA ICVG A GLAFLHEE  P ++HRDIKASNIL
Sbjct: 117 LENNSLDKTLLAGGYTRSGIQFDWSSRANICVGVAKGLAFLHEEVRPHIIHRDIKASNIL 176

Query: 147 LDMNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVL 206
           LD    PKI DFGLA+L P N+THVSTRVAGTIGYLAPEYA+ GQLT+KAD+YSFGV+++
Sbjct: 177 LDKYLSPKISDFGLARLMPPNMTHVSTRVAGTIGYLAPEYAVRGQLTRKADIYSFGVLLM 236

Query: 207 EVISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPELM-EYPKAEVMRFITVALFC 266
           E++S  S+       +   L+E  W+L +   +V+L+D  L   +   E  R++ + L C
Sbjct: 237 EIVSGRSNKNTRLPTEYQYLLERAWELYERNELVDLVDSGLNGVFDAEEACRYLKIGLLC 296

Query: 267 TQAAANQRPNMKQVVEMLSKEVHINEKLLTEPGV 298
           TQ +   RP+M  VV +L+ E  I+ K ++ PG+
Sbjct: 297 TQDSPKLRPSMSTVVRLLTGEKDIDYKKISRPGL 330

BLAST of Sed0000633.1 vs. TAIR 10
Match: AT1G56130.1 (Leucine-rich repeat transmembrane protein kinase )

HSP 1 Score: 289.7 bits (740), Expect = 3.0e-78
Identity = 152/293 (51.88%), Postives = 194/293 (66.21%), Query Frame = 0

Query: 27  QGVLRDGTDVAVKSLSAGSAQGTREFLTEINMISNIRHRNLVELIGCCVEGTHRILVYEY 86
           +G L DG  VAVK LS GS QG  +F+ EI  IS++ HRNLV+L GCC EG HR+LVYEY
Sbjct: 710 KGNLNDGRVVAVKLLSVGSRQGKGQFVAEIVAISSVLHRNLVKLYGCCFEGEHRMLVYEY 769

Query: 87  LENNSLASTLLGTKSKHVILDWPKRAKICVGTALGLAFLHEEAEPTVVHRDIKASNILLD 146
           L N SL   L G K+ H  LDW  R +IC+G A GL +LHEEA   +VHRD+KASNILLD
Sbjct: 770 LPNGSLDQALFGDKTLH--LDWSTRYEICLGVARGLVYLHEEASVRIVHRDVKASNILLD 829

Query: 147 MNFHPKIGDFGLAKLFPDNVTHVSTRVAGTIGYLAPEYALLGQLTKKADVYSFGVVVLEV 206
               P+I DFGLAKL+ D  TH+STRVAGTIGYLAPEYA+ G LT+K DVY+FGVV LE+
Sbjct: 830 SRLVPQISDFGLAKLYDDKKTHISTRVAGTIGYLAPEYAMRGHLTEKTDVYAFGVVALEL 889

Query: 207 ISRTSSSKAAFGEQMSSLVEWTWKLRQEGRVVELIDPELMEYPKAEVMRFITVALFCTQA 266
           +S   +S     E+   L+EW W L ++ R +ELID +L ++   E  R I +AL CTQ 
Sbjct: 890 VSGRPNSDENLEEEKKYLLEWAWNLHEKSRDIELIDDKLTDFNMEEAKRMIGIALLCTQT 949

Query: 267 AANQRPNMKQVVEMLSKEVHINEKLLTEPGVYKTRKFNASAAASSKGSQSSHT 320
           +   RP M +VV MLS +V I + + ++PG     +F+ +  +S  G Q   T
Sbjct: 950 SHALRPPMSRVVAMLSGDVEIGD-VTSKPGYVSDWRFDDTTGSSLSGFQIKDT 999

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145592.11.5e-15386.42cold-responsive protein kinase 1 isoform X2 [Momordica charantia][more]
XP_023527443.11.9e-15185.45cold-responsive protein kinase 1 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022983244.15.5e-15185.45cold-responsive protein kinase 1 isoform X2 [Cucurbita maxima][more]
XP_022934472.18.0e-15084.83cold-responsive protein kinase 1 isoform X2 [Cucurbita moschata][more]
XP_022145585.15.3e-14677.16putative serine/threonine-protein kinase isoform X1 [Momordica charantia] >XP_02... [more]
Match NameE-valueIdentityDescription
C0LGH37.6e-7952.96Probable LRR receptor-like serine/threonine-protein kinase At1g56140 OS=Arabidop... [more]
Q93YN11.7e-7854.38Cold-responsive protein kinase 1 OS=Arabidopsis thaliana OX=3702 GN=CRPK1 PE=1 S... [more]
C0LGH24.2e-7751.88Probable LRR receptor-like serine/threonine-protein kinase At1g56130 OS=Arabidop... [more]
C0LGG85.5e-7749.67Probable LRR receptor-like serine/threonine-protein kinase At1g53430 OS=Arabidop... [more]
C0LGG91.6e-7649.32Probable LRR receptor-like serine/threonine-protein kinase At1g53440 OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1CX227.5e-15486.42cold-responsive protein kinase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1J6S52.7e-15185.45cold-responsive protein kinase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1F2V13.9e-15084.83cold-responsive protein kinase 1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1CWR12.6e-14677.16putative serine/threonine-protein kinase isoform X1 OS=Momordica charantia OX=36... [more]
A0A6J1IYR49.2e-14476.26putative serine/threonine-protein kinase isoform X1 OS=Cucurbita maxima OX=3661 ... [more]
Match NameE-valueIdentityDescription
AT3G09010.11.2e-11977.74Protein kinase superfamily protein [more]
AT1G56120.11.9e-8052.90Leucine-rich repeat transmembrane protein kinase [more]
AT1G56140.15.4e-8052.96Leucine-rich repeat transmembrane protein kinase [more]
AT1G16670.11.2e-7954.38Protein kinase superfamily protein [more]
AT1G56130.13.0e-7851.88Leucine-rich repeat transmembrane protein kinase [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 6..281
e-value: 1.0E-13
score: 61.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 1..292
score: 31.128027
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 88..299
e-value: 4.7E-57
score: 194.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 26..87
e-value: 4.8E-23
score: 83.2
NoneNo IPR availablePANTHERPTHR27006:SF385LEUCINE-RICH REPEAT RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATEDcoord: 28..313
NoneNo IPR availablePANTHERPTHR27006PROMASTIGOTE SURFACE ANTIGEN PROTEIN PSAcoord: 28..313
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 28..283
e-value: 1.00879E-97
score: 287.632
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 32..281
e-value: 2.4E-42
score: 145.0
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 133..145
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 28..281

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Sed0000633Sed0000633gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0000633.1-five_prime_utrSed0000633.1-five_prime_utr-LG02:35602600..35602831five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0000633.1-exonSed0000633.1-exon-LG02:35602600..35602912exon
Sed0000633.1-exonSed0000633.1-exon-LG02:35604006..35604216exon
Sed0000633.1-exonSed0000633.1-exon-LG02:35604783..35605020exon
Sed0000633.1-exonSed0000633.1-exon-LG02:35605368..35605518exon
Sed0000633.1-exonSed0000633.1-exon-LG02:35605608..35606068exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0000633.1-cdsSed0000633.1-cds-LG02:35602832..35602912CDS
Sed0000633.1-cdsSed0000633.1-cds-LG02:35604006..35604216CDS
Sed0000633.1-cdsSed0000633.1-cds-LG02:35604783..35605020CDS
Sed0000633.1-cdsSed0000633.1-cds-LG02:35605368..35605518CDS
Sed0000633.1-cdsSed0000633.1-cds-LG02:35605608..35605895CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0000633.1-three_prime_utrSed0000633.1-three_prime_utr-LG02:35605896..35606068three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Sed0000633.1Sed0000633.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity