Sed0000048.1 (mRNA) Chayote v1

BLAST of Sed0000048.1 vs. NCBI nr
Match: XP_038877776.1 (pectinesterase 2 [Benincasa hispida])

HSP 1 Score: 882.1 bits (2278), Expect = 2.3e-252
Identity = 439/502 (87.45%), Postives = 468/502 (93.23%), Query Frame = 0

Query: 10  VVIILLVFPSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPISNKSHFLKIL 69
           VV+I+L+FP  I+ YSE E+KNWCSQTPYPAPCE FLKTK+TT  K+SPI+ KSHF +IL
Sbjct: 10  VVVIVLIFPLDIYCYSEIEIKNWCSQTPYPAPCEEFLKTKATT--KRSPIATKSHFFEIL 69

Query: 70  VETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRTTDRCSQTDAQTW 129
           VETALE AVSA QNALSLGPKCR +KE+TAWTDCVDLY+QIITRLNRT DRC+  DAQTW
Sbjct: 70  VETALERAVSARQNALSLGPKCRNSKERTAWTDCVDLYDQIITRLNRTVDRCTPVDAQTW 129

Query: 130 LSASLTALDTCRTGFEELGVSAFGYSLTANNVSKLISDGLSVNKPPSPKGFAPPRTEDGF 189
           LSA+LTAL+TCRTG EELGVSAFGY LTANNVSKLISDGLSVNKP SP+GF PP  EDGF
Sbjct: 130 LSAALTALETCRTGLEELGVSAFGYPLTANNVSKLISDGLSVNKPASPEGFEPPTMEDGF 189

Query: 190 PTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAAKGGGRFVIYVKAGVYSENV 249
           PTWVRPGDR+LLQS +P A+ VVA+DGSG+F TVK AVAAAKGGGRFVIYVKAGVYSENV
Sbjct: 190 PTWVRPGDRRLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSENV 249

Query: 250 EINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARDMTFRNTAGASNH 309
           EINAKNVMLIGDGIGK+VITGSRSVGGGSTTFRSATVAVDGD FIARD+TFRNTAGA NH
Sbjct: 250 EINAKNVMLIGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNH 309

Query: 310 QAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDCNI 369
           QAVALRSGSDLSVFYRC FEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDCNI
Sbjct: 310 QAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDCNI 369

Query: 370 IARDPPNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-SGVKAYLGRPWRQYSRTVFMK 429
           IARDPPNKT+T+TAQGRSDPNQNTGISIHNCR+TSAGG SGVKAYLGRPWRQYSRTV MK
Sbjct: 370 IARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWRQYSRTVVMK 429

Query: 430 SSIGGFISPAGWLEWSGNFALNTLYYGEFMNTGAGASTANRVKWKGYRVITSAAEASKFT 489
           SSIGGFISPAGW+EWSGNFALNTLYYGE+MNTG GASTANRVKWKGYRVITSAAEASKFT
Sbjct: 430 SSIGGFISPAGWMEWSGNFALNTLYYGEYMNTGPGASTANRVKWKGYRVITSAAEASKFT 489

Query: 490 AGNFIAGGSWLPGTGVPFTAGL 511
            GNFIAGGSWLP TGVPFT+GL
Sbjct: 490 VGNFIAGGSWLPSTGVPFTSGL 509

BLAST of Sed0000048.1 vs. NCBI nr
Match: XP_022147136.1 (pectinesterase 2 [Momordica charantia])

HSP 1 Score: 864.8 bits (2233), Expect = 3.8e-247
Identity = 437/517 (84.53%), Postives = 473/517 (91.49%), Query Frame = 0

Query: 1   MAAIK---SLSFV-VIILLVFPSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKK 60
           MA IK   SLS V ++++LVFPSAIFGYSE E+K+WCSQTPYPAPCEHFL+TK+ T    
Sbjct: 24  MATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAAT-SNN 83

Query: 61  SPISNKSHFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNR 120
           SPI++KSHFLKILVETAL TAVSAH++A+SLGPKCR ++EK+AW DCV LY+QII+RLNR
Sbjct: 84  SPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNR 143

Query: 121 TTDRCSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTANNVSKLISDGLSVNKPPS 180
           T DRCS++DAQTWLSA+LTAL+TCRTGF+ELGVSA GY LTANNVSKLISDGLSVN PPS
Sbjct: 144 TADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPS 203

Query: 181 PKGFAPPRTEDGFPTWVRPGDRKLLQSAAP--SADAVVAKDGSGDFTTVKAAVAAAKGGG 240
           P GF PP   DGFP WV PGDRKLLQS +P  +AD VVAKDGSGDF TV AAVAAAKGGG
Sbjct: 204 PDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGG 263

Query: 241 RFVIYVKAGVYSENVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFI 300
           RFVIYVKAGVYSENVEI AKNVML+GDGIGK+VITGSRSVGGGSTTFRSATVAVDGD FI
Sbjct: 264 RFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFI 323

Query: 301 ARDMTFRNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVD 360
           ARD+TFRNTAGA NHQAVALRSGSDLSVFYRC FEGFQDTLYVYAERQFYKQCDIYGTVD
Sbjct: 324 ARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVD 383

Query: 361 FIFGNAAVVIQDCNIIARDPPNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-SGVKAY 420
           FIFGNAAVVIQD NIIARDPPNKT+T+TAQGRSDPNQNTGISIHNCRVTSAGG SGVKAY
Sbjct: 384 FIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAY 443

Query: 421 LGRPWRQYSRTVFMKSSIGGFISPAGWLEWSGNFALNTLYYGEFMNTGAGASTANRVKWK 480
           LGRPWRQYSRTVFMKS+IGGFI+PAGW+EWSGNFALNTLYYGE+ NTG G+STANRVKWK
Sbjct: 444 LGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWK 503

Query: 481 GYRVITSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           GY VITSAAEASKFT GNFIAGGSWLP TGVPFT+GL
Sbjct: 504 GYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539

BLAST of Sed0000048.1 vs. NCBI nr
Match: XP_008438193.1 (PREDICTED: pectinesterase 2 [Cucumis melo] >KAA0049055.1 pectinesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 857.4 bits (2214), Expect = 6.1e-245
Identity = 423/512 (82.62%), Postives = 469/512 (91.60%), Query Frame = 0

Query: 1   MAAIKSLSFVVIILLVFPSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPIS 60
           MA    +  VV+++L+FP  +  YSE E+KNWCSQTPYPAPCE FLKTK++T KK +PI+
Sbjct: 1   MARHSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKAST-KKSTPIT 60

Query: 61  NKSHFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRTTDR 120
            KSHF +ILVETALE AVSAH+NALSLGPKCR +KEKTAWTDCVDLY++IITRLNRT+DR
Sbjct: 61  TKSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDR 120

Query: 121 CSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTANNVSKLISDGLSVNKPPSPKGF 180
           C+  DAQTWLSA+LTAL+TCRTGF+ELG+SAFGY LTANNVSKLISDGLSVNKP SP+G+
Sbjct: 121 CTPADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGY 180

Query: 181 APPR-TEDGFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAAKGGGRFVIY 240
            P    +DGFPTWVRPGDRKLLQS +P A+ VVA+DGSG+F TVK AVAAAKGGGRFVIY
Sbjct: 181 EPTTIMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIY 240

Query: 241 VKAGVYSENVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARDMT 300
           VK+GVY+EN++I AKNVM++GDGIGK++ITGSRSVGGGSTTFRSATVAVDGD FIARD+T
Sbjct: 241 VKSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDIT 300

Query: 301 FRNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGN 360
           FRNTAGA NHQAVALRSGSDLSVFYRC FEG+QDTLYVYAERQFYKQCDIYGTVDFIFGN
Sbjct: 301 FRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGN 360

Query: 361 AAVVIQDCNIIARDPPNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-SGVKAYLGRPW 420
           AAVV+QDCNIIARDPPNKT+T+TAQGRSDPNQNTGISIHNCR+TSAGG SGVKAYLGRPW
Sbjct: 361 AAVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPW 420

Query: 421 RQYSRTVFMKSSIGGFISPAGWLEWSGNFALNTLYYGEFMNTGAGASTANRVKWKGYRVI 480
           RQYSRTV MKSSIGGFISPAGW+ WSGNFALNTLYYGE+MNTG GASTANRV WKGYRVI
Sbjct: 421 RQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVI 480

Query: 481 TSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           TSAAEASKFT GNFIAGGSWLP TGVPFT+GL
Sbjct: 481 TSAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of Sed0000048.1 vs. NCBI nr
Match: XP_004133929.1 (pectinesterase 2 [Cucumis sativus] >KGN56617.1 hypothetical protein Csa_010246 [Cucumis sativus])

HSP 1 Score: 856.3 bits (2211), Expect = 1.4e-244
Identity = 422/511 (82.58%), Postives = 466/511 (91.19%), Query Frame = 0

Query: 1   MAAIKSLSFVVIILLVFPSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPIS 60
           MA    + FVV+I+L+FPS I  YSE E+KNWCSQTPYPAPCE FLKTK+TT  KK+PI+
Sbjct: 1   MARHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATT--KKTPIT 60

Query: 61  NKSHFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRTTDR 120
            KSHF +ILVETALE AVSAH+NALSLGPKCR +KEKTAWTDCVDLY+QIITRLNRT+ R
Sbjct: 61  TKSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSAR 120

Query: 121 CSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTANNVSKLISDGLSVNKPPSPKGF 180
           CS  DAQTWLSA+LTAL+TCRTGFEELG+SAFGY LTANNVSKLISDGLSVNKP SP+G+
Sbjct: 121 CSPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGY 180

Query: 181 APPRTEDGFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAAKGGGRFVIYV 240
            P    DGFPTWV PG+RKLLQS +P AD VVA+DGSG+F TVK A++AAKGGGRFVIY+
Sbjct: 181 EPTTMTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYI 240

Query: 241 KAGVYSENVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARDMTF 300
           K+GVY+EN++I AKNVM++GDGIGK++ITGSRSVGGGSTTFRSATVAVDGD FIARD+TF
Sbjct: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300

Query: 301 RNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360
           RNTAGA NHQAVALRSGSDLSVFYRC FEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360

Query: 361 AVVIQDCNIIARDPPNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-SGVKAYLGRPWR 420
           AVV+QDCNIIARDPPNKT+T+TAQGRSDPNQNTGISIHNCR+TS+GG SGVKAYLGRPWR
Sbjct: 361 AVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWR 420

Query: 421 QYSRTVFMKSSIGGFISPAGWLEWSGNFALNTLYYGEFMNTGAGASTANRVKWKGYRVIT 480
           QYSRTV MKSSIGGFISPAGW+ WSGNFALNTLYY E+MNTG GASTANRV WKGY VIT
Sbjct: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVIT 480

Query: 481 SAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           SA+EASKFT GNFIAGGSWLP TGVPFT+GL
Sbjct: 481 SASEASKFTVGNFIAGGSWLPSTGVPFTSGL 509

BLAST of Sed0000048.1 vs. NCBI nr
Match: TYK17508.1 (pectinesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 854.7 bits (2207), Expect = 3.9e-244
Identity = 421/512 (82.23%), Postives = 468/512 (91.41%), Query Frame = 0

Query: 1   MAAIKSLSFVVIILLVFPSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPIS 60
           MA    +  +V+++L+FP  +  YSE E+KNWCSQTPYPAPCE FLKTK++T KK +PI+
Sbjct: 1   MARHSIVFLIVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKAST-KKSTPIT 60

Query: 61  NKSHFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRTTDR 120
            KSHF +ILVETALE AVSAH+NALSLGPKCR +KEKTAWTDCVDLY++IITRLNRT+DR
Sbjct: 61  TKSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDR 120

Query: 121 CSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTANNVSKLISDGLSVNKPPSPKGF 180
           C+  DAQTWLSA+LTAL+TCRTGF+ELG+SAFGY LTANNVSKLISDGLSVNKP SP+G+
Sbjct: 121 CTPADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGY 180

Query: 181 APPR-TEDGFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAAKGGGRFVIY 240
            P    +DGFPTWVRPGDRKLLQS +P A+ VVA+DGSG+F TVK AVAAAK GGRFVIY
Sbjct: 181 EPTTIMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKSGGRFVIY 240

Query: 241 VKAGVYSENVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARDMT 300
           VK+GVY+EN++I AKNVM++GDGIGK++ITGSRSVGGGSTTFRSATVAVDGD FIARD+T
Sbjct: 241 VKSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDIT 300

Query: 301 FRNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGN 360
           FRNTAGA NHQAVALRSGSDLSVFYRC FEG+QDTLYVYAERQFYKQCDIYGTVDFIFGN
Sbjct: 301 FRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGN 360

Query: 361 AAVVIQDCNIIARDPPNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-SGVKAYLGRPW 420
           AAVV+QDCNIIARDPPNKT+T+TAQGRSDPNQNTGISIHNCR+TSAGG SGVKAYLGRPW
Sbjct: 361 AAVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPW 420

Query: 421 RQYSRTVFMKSSIGGFISPAGWLEWSGNFALNTLYYGEFMNTGAGASTANRVKWKGYRVI 480
           RQYSRTV MKSSIGGFISPAGW+ WSGNFALNTLYYGE+MNTG GASTANRV WKGYRVI
Sbjct: 421 RQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVI 480

Query: 481 TSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           TSAAEASKFT GNFIAGGSWLP TGVPFT+GL
Sbjct: 481 TSAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of Sed0000048.1 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 633.6 bits (1633), Expect = 1.9e-180
Identity = 322/512 (62.89%), Postives = 392/512 (76.56%), Query Frame = 0

Query: 7   LSFVVIILLVFPSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPISNKSHFL 66
           ++FV++ +LV  S + GY++ +VK WCSQTP P PCE+FL    T      PI ++S FL
Sbjct: 9   INFVILCILV-ASTVSGYNQKDVKAWCSQTPNPKPCEYFL----THNSNNEPIKSESEFL 68

Query: 67  KILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRTTD---RCSQ 126
           KI ++  L+ A+ A  +A +LGPKCR  +EK AW DC+ LY+  ++++N T D   +CS+
Sbjct: 69  KISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSK 128

Query: 127 TDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTANNVSKLISDGLSVNKPPSPKGFAPP 186
            DAQTWLS +LT LDTCR GF ELGV+     L +NNVS L+ + L++NK P    + PP
Sbjct: 129 LDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKVPF--NYTPP 188

Query: 187 RTEDGFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAAKGGGRFVIYVKAG 246
             +DGFP+WV+PGDRKLLQS+ P  +AVVAKDGSG+F T+K A+ AA G GRFVIYVK G
Sbjct: 189 E-KDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQG 248

Query: 247 VYSENVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARDMTFRNT 306
           VYSEN+EI  KNVML GDGIGK++ITGS+SVGGG+TTF SATVA  GD FIAR +TFRNT
Sbjct: 249 VYSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 308

Query: 307 AGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVV 366
           AGASN QAVALRSGSDLSVFY+CSFE +QDTLYV++ RQFY+ CD+YGTVDFIFGNAA V
Sbjct: 309 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 368

Query: 367 IQDCNIIARDPPNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-----SGVKAYLGRPW 426
           +Q+CNI AR P +KT TITAQGRSDPNQNTGI IHN RVT+A          K YLGRPW
Sbjct: 369 LQNCNIFARRPRSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPW 428

Query: 427 RQYSRTVFMKSSIGGFISPAGWLEWSGNFALNTLYYGEFMNTGAGASTANRVKWKGYRVI 486
           RQYSRTVFMK+S+   I P GWLEW GNFAL TL+Y EF NTG GAST+ RV W G+RV+
Sbjct: 429 RQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVL 488

Query: 487 TSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
            SA+EASKFT G F+AGGSW+P + VPFT+GL
Sbjct: 489 GSASEASKFTVGTFLAGGSWIP-SSVPFTSGL 511

BLAST of Sed0000048.1 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 1.0e-178
Identity = 314/518 (60.62%), Postives = 397/518 (76.64%), Query Frame = 0

Query: 3   AIKSLSFVVIILLVFPSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPISNK 62
           A++ L  V ++L       FGYS  EVK+WC +TP P PCE+FL    T K   + I   
Sbjct: 2   ALRILITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFL----TQKTDVTSIKQD 61

Query: 63  SHFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRTTDR-- 122
           + F KI ++ ALE A +A     +LG KCR  +EK AW DC +LY   + +LN+T++   
Sbjct: 62  TDFYKISLQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSP 121

Query: 123 -CSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTANNVSKLISDGLSVNKPPSPKG 182
            C++ D QTWLS +LT L+TCR   E+LGV  +   L +NNV+KLIS+ LS+NK P    
Sbjct: 122 GCTKVDKQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNKVP---- 181

Query: 183 FAPPRTEDGFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAAK--GGGRFV 242
           +  P  +DGFPTWV+PGDRKLLQ+  P A+ VVA+DGSG+  T++ AVAAA   GG R+V
Sbjct: 182 YNEPSYKDGFPTWVKPGDRKLLQT-TPRANIVVAQDGSGNVKTIQEAVAAASRAGGSRYV 241

Query: 243 IYVKAGVYSENVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARD 302
           IY+KAG Y+EN+E+  KN+M +GDGIGK++ITGS+SVGGG+TTF+SATVAV GD FIARD
Sbjct: 242 IYIKAGTYNENIEVKLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARD 301

Query: 303 MTFRNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIF 362
           +T RNTAG +NHQAVALRSGSDLSVFYRCSFEG+QDTLYV+++RQFY++CDIYGTVDFIF
Sbjct: 302 ITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIF 361

Query: 363 GNAAVVIQDCNIIARDPPNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-----SGVKA 422
           GNAAVV+Q+CNI AR PPN+T T+TAQGR+DPNQ+TGI IHNCRVT+A       S VK 
Sbjct: 362 GNAAVVLQNCNIFARKPPNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKT 421

Query: 423 YLGRPWRQYSRTVFMKSSIGGFISPAGWLEWSGNFALNTLYYGEFMNTGAGASTANRVKW 482
           +LGRPW+QYSRTV++K+ +   I+PAGW+EWSG+FALNTLYY E+MNTG G+STANRVKW
Sbjct: 422 FLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKW 481

Query: 483 KGYRVITSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           +GY V+TS ++ S+FT GNFIAG SWLP T VPFT+GL
Sbjct: 482 RGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of Sed0000048.1 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.7e-120
Identity = 248/534 (46.44%), Postives = 336/534 (62.92%), Query Frame = 0

Query: 6   SLSFVVIILLVF--PSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPISNKS 65
           S+ F+ +ILLV      +   ++    +WC +TPYP PC+ + K  +  ++     +  S
Sbjct: 9   SILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQP----TQLS 68

Query: 66  HFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRT------ 125
            F  +LVE A++ A+SA     + G  C  +K++    DC+DLY   I +LNRT      
Sbjct: 69  EFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSP 128

Query: 126 ----TDRCSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTAN-NVSKLISDGLSVN 185
                  C+  DAQTWLS +LT  +TCR G  +L V+ F   + +N  +S LIS+ L+VN
Sbjct: 129 KAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVN 188

Query: 186 KPPSPKGFAPPRT--EDGFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAA 245
                 G     T  + GFPTW+   D++LL+  A  A+ VVAKDGSG F TV+AA+  A
Sbjct: 189 GALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVA 248

Query: 246 ----KGGGRFVIYVKAGVYSENVEINAKN--VMLIGDGIGKSVITGSRSVGGGSTTFRSA 305
                  GRFVIYVK G+Y EN+ +   N  +ML+GDG+  ++ITG RSV GG TT+ SA
Sbjct: 249 GRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSA 308

Query: 306 TVAVDGDAFIARDMTFRNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFY 365
           T  ++G  FIA+ +TFRNTAG +  QAVALRS SDLS+FY+CS EG+QDTL V+++RQFY
Sbjct: 309 TAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFY 368

Query: 366 KQCDIYGTVDFIFGNAAVVIQDCNIIARDP-PNKTVTITAQGRSDPNQNTGISIHNCRVT 425
           ++C IYGTVDFIFGNAA V Q+C I+ R P   +   ITAQGR+DP QNTGISIHN R+ 
Sbjct: 369 RECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRIL 428

Query: 426 SAGG-----SGVKAYLGRPWRQYSRTVFMKSSIGGFISPAGWLEW--SGNFALNTLYYGE 485
            A         VK Y+GRPW ++SRTV +++ +   +SP GW  W     F L+TL+Y E
Sbjct: 429 PAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAE 488

Query: 486 FMNTGAGASTANRVKWKGYRVITSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           + NTG  +ST  RV WKG+ V+  A++AS FT G FIAG +WLP TG+PFT+GL
Sbjct: 489 YKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Sed0000048.1 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 1.4e-119
Identity = 248/532 (46.62%), Postives = 332/532 (62.41%), Query Frame = 0

Query: 6   SLSFVVIILLVFP--SAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPISNKS 65
           SL  ++I+LL     + +   +   +  WC +TPYP PC+ +    S  +      +  S
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLP----TQIS 72

Query: 66  HFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRTTD---- 125
            F  +LVE A++ AVSA     +    C   K++    DC++LY   + +LNRT      
Sbjct: 73  EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSS 132

Query: 126 ----RCSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTAN-NVSKLISDGLSVNKP 185
               RC+  DAQTWLS +LT  +TCR G  +L VS F   + +N  +S LIS+ L+VN  
Sbjct: 133 KTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGA 192

Query: 186 PSPKGFAPPRTED--GFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAA-- 245
               G     T D  GFPTWV   +R+LLQ  +  A+ VVAKDGSG F TV+AA+  A  
Sbjct: 193 LLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGR 252

Query: 246 --KGGGRFVIYVKAGVYSE--NVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATV 305
                GRFVIYVK G+Y E  NV +N  N+ML+GDG+  ++ITG RSV GG TT+ SAT 
Sbjct: 253 RKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATA 312

Query: 306 AVDGDAFIARDMTFRNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQ 365
            ++G  FIA+ + F+NTAG +  QAVALRS SDLS+FYRCS EG+QDTL V+++RQFY++
Sbjct: 313 GIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRE 372

Query: 366 CDIYGTVDFIFGNAAVVIQDCNIIARDP-PNKTVTITAQGRSDPNQNTGISIHNCRVTSA 425
           C IYGTVDFIFGNAAVV Q+C I+ R P   +   ITAQGR+D  QNTGISIHN  +  A
Sbjct: 373 CYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPA 432

Query: 426 GG-----SGVKAYLGRPWRQYSRTVFMKSSIGGFISPAGWLEWS--GNFALNTLYYGEFM 485
                    VK Y+GRPW  YSRTV +K+ I   +SP GW  W+    + L+TL+Y E+ 
Sbjct: 433 PDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYK 492

Query: 486 NTGAGASTANRVKWKGYRVITSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           N G  +ST  RV+WKG+ V++ A++AS F+ G FIAG +WLPG+G+PFT+ L
Sbjct: 493 NIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of Sed0000048.1 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 7.0e-119
Identity = 240/508 (47.24%), Postives = 320/508 (62.99%), Query Frame = 0

Query: 32  WCSQTPYPAPCEHFLKTKSTTKKKKSPISNKSHFLKILVETALETAVSAHQNALSLGPKC 91
           WC++TP+   C ++ +  S       P   +S FL++LV+ AL+ AV  H   +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 92  RTAKEKTAWTDCVDLYNQIITRLNRTTD----------RCSQTDAQTWLSASLTALDTCR 151
              + K AW+DCV+L+   + +LNRT            +C+  DAQTWLS + T ++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 152 TGFEELGVSAFGYSLTAN-NVSKLISDGLSVN--KPPSPKGFAPPRTEDGFPTWVRPGDR 211
           +G E+L VS F   + +N N+S LI + L+VN               ++ FP+WV   +R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 212 KLLQSAA---PSADAVVAKDGSGDFTTVKAAV---AAAKGGGRFVIYVKAGVYSENVEI- 271
           +LL SA+    S   VVA+D SG F +++AA+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 272 -NAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARDMTFRNTAGASNHQ 331
            +  N+ML+GDG  K++IT  RSV  G TT+ SAT    G  F+A+DMTF NTAG    Q
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLRGQ 386

Query: 332 AVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDCNII 391
           AVA+RS SDLSVFYR    GFQDTLY++++RQF+++C I GT+DFIFGNAAVV Q+C I+
Sbjct: 387 AVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCMIL 446

Query: 392 ARDP-PNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-----SGVKAYLGRPWRQYSRT 451
            R P   +   ITAQGR DP QNTGI+IH+ R+ +A          K YLGRPW+ YSR 
Sbjct: 447 VRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYSRV 506

Query: 452 VFMKSSIGGFISPAGWLEW--SGNFALNTLYYGEFMNTGAGASTANRVKWKGYRVITSAA 511
             MK+ I   ISP GW  W    NFALNT++YGE+ N G G+ST  RV+WKG+  ITS A
Sbjct: 507 TIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITSTA 566

BLAST of Sed0000048.1 vs. ExPASy TrEMBL
Match: A0A6J1D1F9 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016139 PE=3 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 1.8e-247
Identity = 437/517 (84.53%), Postives = 473/517 (91.49%), Query Frame = 0

Query: 1   MAAIK---SLSFV-VIILLVFPSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKK 60
           MA IK   SLS V ++++LVFPSAIFGYSE E+K+WCSQTPYPAPCEHFL+TK+ T    
Sbjct: 24  MATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAAT-SNN 83

Query: 61  SPISNKSHFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNR 120
           SPI++KSHFLKILVETAL TAVSAH++A+SLGPKCR ++EK+AW DCV LY+QII+RLNR
Sbjct: 84  SPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNR 143

Query: 121 TTDRCSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTANNVSKLISDGLSVNKPPS 180
           T DRCS++DAQTWLSA+LTAL+TCRTGF+ELGVSA GY LTANNVSKLISDGLSVN PPS
Sbjct: 144 TADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPS 203

Query: 181 PKGFAPPRTEDGFPTWVRPGDRKLLQSAAP--SADAVVAKDGSGDFTTVKAAVAAAKGGG 240
           P GF PP   DGFP WV PGDRKLLQS +P  +AD VVAKDGSGDF TV AAVAAAKGGG
Sbjct: 204 PDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGG 263

Query: 241 RFVIYVKAGVYSENVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFI 300
           RFVIYVKAGVYSENVEI AKNVML+GDGIGK+VITGSRSVGGGSTTFRSATVAVDGD FI
Sbjct: 264 RFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFI 323

Query: 301 ARDMTFRNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVD 360
           ARD+TFRNTAGA NHQAVALRSGSDLSVFYRC FEGFQDTLYVYAERQFYKQCDIYGTVD
Sbjct: 324 ARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVD 383

Query: 361 FIFGNAAVVIQDCNIIARDPPNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-SGVKAY 420
           FIFGNAAVVIQD NIIARDPPNKT+T+TAQGRSDPNQNTGISIHNCRVTSAGG SGVKAY
Sbjct: 384 FIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAY 443

Query: 421 LGRPWRQYSRTVFMKSSIGGFISPAGWLEWSGNFALNTLYYGEFMNTGAGASTANRVKWK 480
           LGRPWRQYSRTVFMKS+IGGFI+PAGW+EWSGNFALNTLYYGE+ NTG G+STANRVKWK
Sbjct: 444 LGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWK 503

Query: 481 GYRVITSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           GY VITSAAEASKFT GNFIAGGSWLP TGVPFT+GL
Sbjct: 504 GYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539

BLAST of Sed0000048.1 vs. ExPASy TrEMBL
Match: A0A5A7U603 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002830 PE=3 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.9e-245
Identity = 423/512 (82.62%), Postives = 469/512 (91.60%), Query Frame = 0

Query: 1   MAAIKSLSFVVIILLVFPSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPIS 60
           MA    +  VV+++L+FP  +  YSE E+KNWCSQTPYPAPCE FLKTK++T KK +PI+
Sbjct: 1   MARHSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKAST-KKSTPIT 60

Query: 61  NKSHFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRTTDR 120
            KSHF +ILVETALE AVSAH+NALSLGPKCR +KEKTAWTDCVDLY++IITRLNRT+DR
Sbjct: 61  TKSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDR 120

Query: 121 CSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTANNVSKLISDGLSVNKPPSPKGF 180
           C+  DAQTWLSA+LTAL+TCRTGF+ELG+SAFGY LTANNVSKLISDGLSVNKP SP+G+
Sbjct: 121 CTPADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGY 180

Query: 181 APPR-TEDGFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAAKGGGRFVIY 240
            P    +DGFPTWVRPGDRKLLQS +P A+ VVA+DGSG+F TVK AVAAAKGGGRFVIY
Sbjct: 181 EPTTIMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIY 240

Query: 241 VKAGVYSENVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARDMT 300
           VK+GVY+EN++I AKNVM++GDGIGK++ITGSRSVGGGSTTFRSATVAVDGD FIARD+T
Sbjct: 241 VKSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDIT 300

Query: 301 FRNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGN 360
           FRNTAGA NHQAVALRSGSDLSVFYRC FEG+QDTLYVYAERQFYKQCDIYGTVDFIFGN
Sbjct: 301 FRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGN 360

Query: 361 AAVVIQDCNIIARDPPNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-SGVKAYLGRPW 420
           AAVV+QDCNIIARDPPNKT+T+TAQGRSDPNQNTGISIHNCR+TSAGG SGVKAYLGRPW
Sbjct: 361 AAVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPW 420

Query: 421 RQYSRTVFMKSSIGGFISPAGWLEWSGNFALNTLYYGEFMNTGAGASTANRVKWKGYRVI 480
           RQYSRTV MKSSIGGFISPAGW+ WSGNFALNTLYYGE+MNTG GASTANRV WKGYRVI
Sbjct: 421 RQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVI 480

Query: 481 TSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           TSAAEASKFT GNFIAGGSWLP TGVPFT+GL
Sbjct: 481 TSAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of Sed0000048.1 vs. ExPASy TrEMBL
Match: A0A1S3AVW4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483370 PE=3 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.9e-245
Identity = 423/512 (82.62%), Postives = 469/512 (91.60%), Query Frame = 0

Query: 1   MAAIKSLSFVVIILLVFPSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPIS 60
           MA    +  VV+++L+FP  +  YSE E+KNWCSQTPYPAPCE FLKTK++T KK +PI+
Sbjct: 1   MARHSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKAST-KKSTPIT 60

Query: 61  NKSHFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRTTDR 120
            KSHF +ILVETALE AVSAH+NALSLGPKCR +KEKTAWTDCVDLY++IITRLNRT+DR
Sbjct: 61  TKSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDR 120

Query: 121 CSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTANNVSKLISDGLSVNKPPSPKGF 180
           C+  DAQTWLSA+LTAL+TCRTGF+ELG+SAFGY LTANNVSKLISDGLSVNKP SP+G+
Sbjct: 121 CTPADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGY 180

Query: 181 APPR-TEDGFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAAKGGGRFVIY 240
            P    +DGFPTWVRPGDRKLLQS +P A+ VVA+DGSG+F TVK AVAAAKGGGRFVIY
Sbjct: 181 EPTTIMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIY 240

Query: 241 VKAGVYSENVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARDMT 300
           VK+GVY+EN++I AKNVM++GDGIGK++ITGSRSVGGGSTTFRSATVAVDGD FIARD+T
Sbjct: 241 VKSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDIT 300

Query: 301 FRNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGN 360
           FRNTAGA NHQAVALRSGSDLSVFYRC FEG+QDTLYVYAERQFYKQCDIYGTVDFIFGN
Sbjct: 301 FRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGN 360

Query: 361 AAVVIQDCNIIARDPPNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-SGVKAYLGRPW 420
           AAVV+QDCNIIARDPPNKT+T+TAQGRSDPNQNTGISIHNCR+TSAGG SGVKAYLGRPW
Sbjct: 361 AAVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPW 420

Query: 421 RQYSRTVFMKSSIGGFISPAGWLEWSGNFALNTLYYGEFMNTGAGASTANRVKWKGYRVI 480
           RQYSRTV MKSSIGGFISPAGW+ WSGNFALNTLYYGE+MNTG GASTANRV WKGYRVI
Sbjct: 421 RQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVI 480

Query: 481 TSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           TSAAEASKFT GNFIAGGSWLP TGVPFT+GL
Sbjct: 481 TSAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of Sed0000048.1 vs. ExPASy TrEMBL
Match: A0A0A0L7N4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126810 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 6.6e-245
Identity = 422/511 (82.58%), Postives = 466/511 (91.19%), Query Frame = 0

Query: 1   MAAIKSLSFVVIILLVFPSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPIS 60
           MA    + FVV+I+L+FPS I  YSE E+KNWCSQTPYPAPCE FLKTK+TT  KK+PI+
Sbjct: 1   MARHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATT--KKTPIT 60

Query: 61  NKSHFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRTTDR 120
            KSHF +ILVETALE AVSAH+NALSLGPKCR +KEKTAWTDCVDLY+QIITRLNRT+ R
Sbjct: 61  TKSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSAR 120

Query: 121 CSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTANNVSKLISDGLSVNKPPSPKGF 180
           CS  DAQTWLSA+LTAL+TCRTGFEELG+SAFGY LTANNVSKLISDGLSVNKP SP+G+
Sbjct: 121 CSPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGY 180

Query: 181 APPRTEDGFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAAKGGGRFVIYV 240
            P    DGFPTWV PG+RKLLQS +P AD VVA+DGSG+F TVK A++AAKGGGRFVIY+
Sbjct: 181 EPTTMTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYI 240

Query: 241 KAGVYSENVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARDMTF 300
           K+GVY+EN++I AKNVM++GDGIGK++ITGSRSVGGGSTTFRSATVAVDGD FIARD+TF
Sbjct: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300

Query: 301 RNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360
           RNTAGA NHQAVALRSGSDLSVFYRC FEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360

Query: 361 AVVIQDCNIIARDPPNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-SGVKAYLGRPWR 420
           AVV+QDCNIIARDPPNKT+T+TAQGRSDPNQNTGISIHNCR+TS+GG SGVKAYLGRPWR
Sbjct: 361 AVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWR 420

Query: 421 QYSRTVFMKSSIGGFISPAGWLEWSGNFALNTLYYGEFMNTGAGASTANRVKWKGYRVIT 480
           QYSRTV MKSSIGGFISPAGW+ WSGNFALNTLYY E+MNTG GASTANRV WKGY VIT
Sbjct: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVIT 480

Query: 481 SAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           SA+EASKFT GNFIAGGSWLP TGVPFT+GL
Sbjct: 481 SASEASKFTVGNFIAGGSWLPSTGVPFTSGL 509

BLAST of Sed0000048.1 vs. ExPASy TrEMBL
Match: A0A5D3D1Y4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003750 PE=3 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 1.9e-244
Identity = 421/512 (82.23%), Postives = 468/512 (91.41%), Query Frame = 0

Query: 1   MAAIKSLSFVVIILLVFPSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPIS 60
           MA    +  +V+++L+FP  +  YSE E+KNWCSQTPYPAPCE FLKTK++T KK +PI+
Sbjct: 1   MARHSIVFLIVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKAST-KKSTPIT 60

Query: 61  NKSHFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRTTDR 120
            KSHF +ILVETALE AVSAH+NALSLGPKCR +KEKTAWTDCVDLY++IITRLNRT+DR
Sbjct: 61  TKSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDR 120

Query: 121 CSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTANNVSKLISDGLSVNKPPSPKGF 180
           C+  DAQTWLSA+LTAL+TCRTGF+ELG+SAFGY LTANNVSKLISDGLSVNKP SP+G+
Sbjct: 121 CTPADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGY 180

Query: 181 APPR-TEDGFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAAKGGGRFVIY 240
            P    +DGFPTWVRPGDRKLLQS +P A+ VVA+DGSG+F TVK AVAAAK GGRFVIY
Sbjct: 181 EPTTIMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKSGGRFVIY 240

Query: 241 VKAGVYSENVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARDMT 300
           VK+GVY+EN++I AKNVM++GDGIGK++ITGSRSVGGGSTTFRSATVAVDGD FIARD+T
Sbjct: 241 VKSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDIT 300

Query: 301 FRNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGN 360
           FRNTAGA NHQAVALRSGSDLSVFYRC FEG+QDTLYVYAERQFYKQCDIYGTVDFIFGN
Sbjct: 301 FRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGN 360

Query: 361 AAVVIQDCNIIARDPPNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-SGVKAYLGRPW 420
           AAVV+QDCNIIARDPPNKT+T+TAQGRSDPNQNTGISIHNCR+TSAGG SGVKAYLGRPW
Sbjct: 361 AAVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPW 420

Query: 421 RQYSRTVFMKSSIGGFISPAGWLEWSGNFALNTLYYGEFMNTGAGASTANRVKWKGYRVI 480
           RQYSRTV MKSSIGGFISPAGW+ WSGNFALNTLYYGE+MNTG GASTANRV WKGYRVI
Sbjct: 421 RQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVI 480

Query: 481 TSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           TSAAEASKFT GNFIAGGSWLP TGVPFT+GL
Sbjct: 481 TSAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of Sed0000048.1 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 633.6 bits (1633), Expect = 1.3e-181
Identity = 322/512 (62.89%), Postives = 392/512 (76.56%), Query Frame = 0

Query: 7   LSFVVIILLVFPSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPISNKSHFL 66
           ++FV++ +LV  S + GY++ +VK WCSQTP P PCE+FL    T      PI ++S FL
Sbjct: 9   INFVILCILV-ASTVSGYNQKDVKAWCSQTPNPKPCEYFL----THNSNNEPIKSESEFL 68

Query: 67  KILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRTTD---RCSQ 126
           KI ++  L+ A+ A  +A +LGPKCR  +EK AW DC+ LY+  ++++N T D   +CS+
Sbjct: 69  KISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSK 128

Query: 127 TDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTANNVSKLISDGLSVNKPPSPKGFAPP 186
            DAQTWLS +LT LDTCR GF ELGV+     L +NNVS L+ + L++NK P    + PP
Sbjct: 129 LDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKVPF--NYTPP 188

Query: 187 RTEDGFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAAKGGGRFVIYVKAG 246
             +DGFP+WV+PGDRKLLQS+ P  +AVVAKDGSG+F T+K A+ AA G GRFVIYVK G
Sbjct: 189 E-KDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQG 248

Query: 247 VYSENVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARDMTFRNT 306
           VYSEN+EI  KNVML GDGIGK++ITGS+SVGGG+TTF SATVA  GD FIAR +TFRNT
Sbjct: 249 VYSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 308

Query: 307 AGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVV 366
           AGASN QAVALRSGSDLSVFY+CSFE +QDTLYV++ RQFY+ CD+YGTVDFIFGNAA V
Sbjct: 309 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 368

Query: 367 IQDCNIIARDPPNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-----SGVKAYLGRPW 426
           +Q+CNI AR P +KT TITAQGRSDPNQNTGI IHN RVT+A          K YLGRPW
Sbjct: 369 LQNCNIFARRPRSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPW 428

Query: 427 RQYSRTVFMKSSIGGFISPAGWLEWSGNFALNTLYYGEFMNTGAGASTANRVKWKGYRVI 486
           RQYSRTVFMK+S+   I P GWLEW GNFAL TL+Y EF NTG GAST+ RV W G+RV+
Sbjct: 429 RQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVL 488

Query: 487 TSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
            SA+EASKFT G F+AGGSW+P + VPFT+GL
Sbjct: 489 GSASEASKFTVGTFLAGGSWIP-SSVPFTSGL 511

BLAST of Sed0000048.1 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 434.5 bits (1116), Expect = 1.2e-121
Identity = 248/534 (46.44%), Postives = 336/534 (62.92%), Query Frame = 0

Query: 6   SLSFVVIILLVF--PSAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPISNKS 65
           S+ F+ +ILLV      +   ++    +WC +TPYP PC+ + K  +  ++     +  S
Sbjct: 9   SILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQP----TQLS 68

Query: 66  HFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRT------ 125
            F  +LVE A++ A+SA     + G  C  +K++    DC+DLY   I +LNRT      
Sbjct: 69  EFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSP 128

Query: 126 ----TDRCSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTAN-NVSKLISDGLSVN 185
                  C+  DAQTWLS +LT  +TCR G  +L V+ F   + +N  +S LIS+ L+VN
Sbjct: 129 KAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVN 188

Query: 186 KPPSPKGFAPPRT--EDGFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAA 245
                 G     T  + GFPTW+   D++LL+  A  A+ VVAKDGSG F TV+AA+  A
Sbjct: 189 GALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVA 248

Query: 246 ----KGGGRFVIYVKAGVYSENVEINAKN--VMLIGDGIGKSVITGSRSVGGGSTTFRSA 305
                  GRFVIYVK G+Y EN+ +   N  +ML+GDG+  ++ITG RSV GG TT+ SA
Sbjct: 249 GRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSA 308

Query: 306 TVAVDGDAFIARDMTFRNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFY 365
           T  ++G  FIA+ +TFRNTAG +  QAVALRS SDLS+FY+CS EG+QDTL V+++RQFY
Sbjct: 309 TAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFY 368

Query: 366 KQCDIYGTVDFIFGNAAVVIQDCNIIARDP-PNKTVTITAQGRSDPNQNTGISIHNCRVT 425
           ++C IYGTVDFIFGNAA V Q+C I+ R P   +   ITAQGR+DP QNTGISIHN R+ 
Sbjct: 369 RECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRIL 428

Query: 426 SAGG-----SGVKAYLGRPWRQYSRTVFMKSSIGGFISPAGWLEW--SGNFALNTLYYGE 485
            A         VK Y+GRPW ++SRTV +++ +   +SP GW  W     F L+TL+Y E
Sbjct: 429 PAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAE 488

Query: 486 FMNTGAGASTANRVKWKGYRVITSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           + NTG  +ST  RV WKG+ V+  A++AS FT G FIAG +WLP TG+PFT+GL
Sbjct: 489 YKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Sed0000048.1 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 431.4 bits (1108), Expect = 1.0e-120
Identity = 248/532 (46.62%), Postives = 332/532 (62.41%), Query Frame = 0

Query: 6   SLSFVVIILLVFP--SAIFGYSENEVKNWCSQTPYPAPCEHFLKTKSTTKKKKSPISNKS 65
           SL  ++I+LL     + +   +   +  WC +TPYP PC+ +    S  +      +  S
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLP----TQIS 72

Query: 66  HFLKILVETALETAVSAHQNALSLGPKCRTAKEKTAWTDCVDLYNQIITRLNRTTD---- 125
            F  +LVE A++ AVSA     +    C   K++    DC++LY   + +LNRT      
Sbjct: 73  EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSS 132

Query: 126 ----RCSQTDAQTWLSASLTALDTCRTGFEELGVSAFGYSLTAN-NVSKLISDGLSVNKP 185
               RC+  DAQTWLS +LT  +TCR G  +L VS F   + +N  +S LIS+ L+VN  
Sbjct: 133 KTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGA 192

Query: 186 PSPKGFAPPRTED--GFPTWVRPGDRKLLQSAAPSADAVVAKDGSGDFTTVKAAVAAA-- 245
               G     T D  GFPTWV   +R+LLQ  +  A+ VVAKDGSG F TV+AA+  A  
Sbjct: 193 LLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGR 252

Query: 246 --KGGGRFVIYVKAGVYSE--NVEINAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATV 305
                GRFVIYVK G+Y E  NV +N  N+ML+GDG+  ++ITG RSV GG TT+ SAT 
Sbjct: 253 RKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATA 312

Query: 306 AVDGDAFIARDMTFRNTAGASNHQAVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQ 365
            ++G  FIA+ + F+NTAG +  QAVALRS SDLS+FYRCS EG+QDTL V+++RQFY++
Sbjct: 313 GIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRE 372

Query: 366 CDIYGTVDFIFGNAAVVIQDCNIIARDP-PNKTVTITAQGRSDPNQNTGISIHNCRVTSA 425
           C IYGTVDFIFGNAAVV Q+C I+ R P   +   ITAQGR+D  QNTGISIHN  +  A
Sbjct: 373 CYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPA 432

Query: 426 GG-----SGVKAYLGRPWRQYSRTVFMKSSIGGFISPAGWLEWS--GNFALNTLYYGEFM 485
                    VK Y+GRPW  YSRTV +K+ I   +SP GW  W+    + L+TL+Y E+ 
Sbjct: 433 PDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYK 492

Query: 486 NTGAGASTANRVKWKGYRVITSAAEASKFTAGNFIAGGSWLPGTGVPFTAGL 511
           N G  +ST  RV+WKG+ V++ A++AS F+ G FIAG +WLPG+G+PFT+ L
Sbjct: 493 NIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of Sed0000048.1 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 429.1 bits (1102), Expect = 5.0e-120
Identity = 240/508 (47.24%), Postives = 320/508 (62.99%), Query Frame = 0

Query: 32  WCSQTPYPAPCEHFLKTKSTTKKKKSPISNKSHFLKILVETALETAVSAHQNALSLGPKC 91
           WC++TP+   C ++ +  S       P   +S FL++LV+ AL+ AV  H   +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 92  RTAKEKTAWTDCVDLYNQIITRLNRTTD----------RCSQTDAQTWLSASLTALDTCR 151
              + K AW+DCV+L+   + +LNRT            +C+  DAQTWLS + T ++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 152 TGFEELGVSAFGYSLTAN-NVSKLISDGLSVN--KPPSPKGFAPPRTEDGFPTWVRPGDR 211
           +G E+L VS F   + +N N+S LI + L+VN               ++ FP+WV   +R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 212 KLLQSAA---PSADAVVAKDGSGDFTTVKAAV---AAAKGGGRFVIYVKAGVYSENVEI- 271
           +LL SA+    S   VVA+D SG F +++AA+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 272 -NAKNVMLIGDGIGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARDMTFRNTAGASNHQ 331
            +  N+ML+GDG  K++IT  RSV  G TT+ SAT    G  F+A+DMTF NTAG    Q
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLRGQ 386

Query: 332 AVALRSGSDLSVFYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDCNII 391
           AVA+RS SDLSVFYR    GFQDTLY++++RQF+++C I GT+DFIFGNAAVV Q+C I+
Sbjct: 387 AVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCMIL 446

Query: 392 ARDP-PNKTVTITAQGRSDPNQNTGISIHNCRVTSAGG-----SGVKAYLGRPWRQYSRT 451
            R P   +   ITAQGR DP QNTGI+IH+ R+ +A          K YLGRPW+ YSR 
Sbjct: 447 VRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYSRV 506

Query: 452 VFMKSSIGGFISPAGWLEW--SGNFALNTLYYGEFMNTGAGASTANRVKWKGYRVITSAA 511
             MK+ I   ISP GW  W    NFALNT++YGE+ N G G+ST  RV+WKG+  ITS A
Sbjct: 507 TIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITSTA 566

BLAST of Sed0000048.1 vs. TAIR 10
Match: AT4G00190.1 (pectin methylesterase 38 )

HSP 1 Score: 423.7 bits (1088), Expect = 2.1e-118
Identity = 239/491 (48.68%), Postives = 311/491 (63.34%), Query Frame = 0

Query: 33  CSQTPYPAPCEHFLKTKSTTKKKKSPISNKSHFLKILVETALETAVSAHQNALSLGPKCR 92
           C +TP+P  C+       T   K  PI + + FL++ VE  L+ AV A  +   L P+  
Sbjct: 8   CDETPHPGECK-------TLLIKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFG 67

Query: 93  TAKEKTAWTDCVDLYNQIITRLNRTT----DRCSQTDAQTWLSASLTALDTCRTGFEELG 152
           +   K AW +C+DLY Q I RLN +     + CS++D Q WLS +LT LDTC+    ELG
Sbjct: 68  S---KQAWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELG 127

Query: 153 VSAFGYSLTANNVSKLISDGLSVNKPPSPKGFAPPRTEDGFPTWVRPGDRKLLQSAAPSA 212
           VS+  +SL +  +   + + L++NK            ++G    +     K L S     
Sbjct: 128 VSS--HSLESITID--VINTLAINK---------RMEQNGKEFGISKITMKTL-SIGEKV 187

Query: 213 DAVVAKDGSGDFTTVKAAVAAA----KGGGRFVIYVKAGVYSE--NVEINAKNVMLIGDG 272
           D VVA+DGSGD+ T++ AV  A    KG  R+VI+VK GVY E  NV I + N+M+ GDG
Sbjct: 188 DVVVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMITGDG 247

Query: 273 IGKSVITGSRSVGGGSTTFRSATVAVDGDAFIARDMTFRNTAGASNHQAVALRSGSDLSV 332
           IGK++ITG +S G G +T++SAT   +GD F+ RD+T RNTAG  NHQAVALRS SD+SV
Sbjct: 248 IGKTIITGDKSKGRGFSTYKSATFVAEGDGFVGRDITIRNTAGPENHQAVALRSNSDMSV 307

Query: 333 FYRCSFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDCNIIARDPPNKTVTIT 392
           FYRCS EG+QDTLYV++ RQF+++CDIYGTVDFIFGNAA V+Q+C I AR+PPN   TIT
Sbjct: 308 FYRCSIEGYQDTLYVHSGRQFFRECDIYGTVDFIFGNAAAVLQNCRIFARNPPNGVNTIT 367

Query: 393 AQGRSDPNQNTGISIHNCRVTSAGG---SGVKAYLGRPWRQYSRTVFMKSSIGGFISPAG 452
           AQ R +PNQ TGI IHN  V  A G    GVK YLGRPWR Y+RTV + + +   I P G
Sbjct: 368 AQSRFNPNQTTGIVIHNSVVKGAPGVQLGGVKTYLGRPWRSYARTVVIGTYLDTLIEPNG 427

Query: 453 WLEWSGNFALNTLYYGEFMNTGAGASTANRVKWKGYRVITSAAEASKFTAGNFIAGGSWL 511
           W++W    AL+TLYYGE+ N+G G+ T NRV W G+ VI+   EA +FT   FI   SWL
Sbjct: 428 WIDWDNVTALSTLYYGEYQNSGPGSGTENRVDWAGFHVISDIQEAREFTLPKFIDSASWL 474