Pay0016163.1 (mRNA) Melon (Payzawat) v1

Overview


Name	Pay0016163.1
Type	mRNA
Organism	Cucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description	ATP-dependent Clp protease proteolytic subunit
Location	chr09: 14235865 .. 14236569 (-)
Sequence length	339
RNA-Seq Expression	Pay0016163.1
Synteny	Pay0016163.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCCCGTCACCACATCTCAGCCGCTGCCTCAATCGTTCGCTGCCAGTGCTGACTCCAGTCGCAAGCTCAACTAAATCTGACTCCTGCTGGTGCCGTGATGCTAATTCCAACCGTGTTGCAACTCCTGACGCCACCAAGATCTGACTCCAGCCACCTCCTTCTCGGCTCACGCTGCCGCACACTGCCGCATACTATCAGACAATGTTTCCAATTCGAAGCCTTTCAGTTTCCGTTGCAAAGCTACAAATTACTTTACTCCCTCCATCCTCATTTGCCTCCTCCATCTCCATCGGCCCTAATCTCCAAATCCCACAATAACATCCCAATTTGAAGGACTTCTTCTCTTATATTGATACTTTTGTTTGCTTTTGGATTAGAGAATTATTCGTTGTGGTGGAGCCCTTGATGATGATATGTCGAATATCATTGTTGCTCAACTTCTATACCTTGATGCTATTGATCCCAATGAGGTTCTCTTTCTTTTCTCTCTATTCATTTTAAGGAAGTAGCTCATATATAGTTCGTCAGGTTTTCTTATAACTGATTAACATAATCTTTTGGTATGCTTAACAGGATATTATGATGTATGTAAATTCCCGTGTTGAGTTACAGCTGGTAATATTGAAACCGGTGGAAACCATATCACTGATTGTTTGTTCTGTTGGAAACTGGGACGAGCTTTATCTTTTAACTACTGATTAA

mRNA sequence

ATGCCCCCGTCACCACATCTCAGCCGCTGCCTCAATCGTTCGCTGCCAGTGCTGACTCCAGTCGCAAGCTCAACTAAATCTGACTCCTGCTGGTGCCGTGATGCTAATTCCAACCGTAGAATTATTCGTTGTGGTGGAGCCCTTGATGATGATATGTCGAATATCATTGTTGCTCAACTTCTATACCTTGATGCTATTGATCCCAATGAGGATATTATGATGTATGTAAATTCCCGTGTTGAGTTACAGCTGGTAATATTGAAACCGGTGGAAACCATATCACTGATTGTTTGTTCTGTTGGAAACTGGGACGAGCTTTATCTTTTAACTACTGATTAA

Coding sequence (CDS)

ATGCCCCCGTCACCACATCTCAGCCGCTGCCTCAATCGTTCGCTGCCAGTGCTGACTCCAGTCGCAAGCTCAACTAAATCTGACTCCTGCTGGTGCCGTGATGCTAATTCCAACCGTAGAATTATTCGTTGTGGTGGAGCCCTTGATGATGATATGTCGAATATCATTGTTGCTCAACTTCTATACCTTGATGCTATTGATCCCAATGAGGATATTATGATGTATGTAAATTCCCGTGTTGAGTTACAGCTGGTAATATTGAAACCGGTGGAAACCATATCACTGATTGTTTGTTCTGTTGGAAACTGGGACGAGCTTTATCTTTTAACTACTGATTAA

Protein sequence

MPPSPHLSRCLNRSLPVLTPVASSTKSDSCWCRDANSNRRIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNSRVELQLVILKPVETISLIVCSVGNWDELYLLTTD

Homology

[Close]

BLAST of Pay0016163.1 vs. ExPASy Swiss-Prot
Match: Q9S834 (ATP-dependent Clp protease proteolytic subunit 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLPP5 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 2.8e-12
Identity = 33/39 (84.62%), Postives = 38/39 (97.44%), Query Frame = 0


Query: 40  RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
           RIIRCGGA+DDDM+NIIVAQLLYLDA+DP +DI+MYVNS
Sbjct: 123 RIIRCGGAVDDDMANIIVAQLLYLDAVDPTKDIVMYVNS 161

[Close]

BLAST of Pay0016163.1 vs. ExPASy Swiss-Prot
Match: B9M0Y1 (ATP-dependent Clp protease proteolytic subunit OS=Geobacter daltonii (strain DSM 22248 / JCM 15807 / FRC-32) OX=316067 GN=clpP PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.3e-06
Identity = 23/39 (58.97%), Postives = 34/39 (87.18%), Query Frame = 0


Query: 40 RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
          RII  GGA+DD +SN+++AQLL+L+A DP++DI +Y+NS
Sbjct: 27 RIIFLGGAIDDTVSNLVIAQLLFLEAEDPDKDIHLYINS 65

[Close]

BLAST of Pay0016163.1 vs. ExPASy Swiss-Prot
Match: A5GFA0 (ATP-dependent Clp protease proteolytic subunit OS=Geobacter uraniireducens (strain Rf4) OX=351605 GN=clpP PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 2.3e-06
Identity = 22/39 (56.41%), Postives = 34/39 (87.18%), Query Frame = 0


Query: 40 RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
          RI+  GGA+DD +SN+++AQLL+L+A DP++DI +Y+NS
Sbjct: 27 RIVFLGGAIDDAISNLVIAQLLFLEAEDPDKDIHLYINS 65

[Close]

BLAST of Pay0016163.1 vs. ExPASy Swiss-Prot
Match: B5EI27 (ATP-dependent Clp protease proteolytic subunit OS=Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) OX=404380 GN=clpP PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 5.1e-06
Identity = 21/39 (53.85%), Postives = 34/39 (87.18%), Query Frame = 0


Query: 40 RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
          RII  GG +DD+++N+++AQLL+L+A DP++DI +Y+NS
Sbjct: 27 RIIFLGGGIDDNVANLVIAQLLFLEAEDPDKDIHLYINS 65

[Close]

BLAST of Pay0016163.1 vs. ExPASy Swiss-Prot
Match: C6E2T0 (ATP-dependent Clp protease proteolytic subunit OS=Geobacter sp. (strain M21) OX=443144 GN=clpP PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 5.1e-06
Identity = 21/39 (53.85%), Postives = 34/39 (87.18%), Query Frame = 0


Query: 40 RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
          RII  GG +DD+++N+++AQLL+L+A DP++DI +Y+NS
Sbjct: 27 RIIFLGGGIDDNVANLVIAQLLFLEAEDPDKDIHLYINS 65

[Close]

BLAST of Pay0016163.1 vs. ExPASy TrEMBL
Match: A0A1S3BPQ7 (uncharacterized protein LOC103491874 OS=Cucumis melo OX=3656 GN=LOC103491874 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.4e-14
Identity = 49/69 (71.01%), Postives = 54/69 (78.26%), Query Frame = 0


Query: 12 NRSLPVLTPVASSTKSDSCWCRDANSNR--------RIIRCGGALDDDMSNIIVAQLLYL 71
          +R  P+ TPVASSTKSDS   RDANS+R        RIIRCGGALDDDM+NIIVAQLLYL
Sbjct: 23 HRMPPMPTPVASSTKSDSYCYRDANSSRVATSYATQRIIRCGGALDDDMANIIVAQLLYL 82

Query: 72 DAIDPNEDI 73
          DAIDPN+ I
Sbjct: 83 DAIDPNKKI 91

[Close]

BLAST of Pay0016163.1 vs. ExPASy TrEMBL
Match: A0A445FJU8 (ATP-dependent Clp protease proteolytic subunit 5, chloroplastic isoform A OS=Glycine soja OX=3848 GN=D0Y65_052205 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 9.3e-11
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 0


Query: 40 RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
          RI+RCGGA+DDDMSNIIVAQLLYLDAIDPN+DI+MYVNS
Sbjct: 42 RIVRCGGAVDDDMSNIIVAQLLYLDAIDPNKDIVMYVNS 80

[Close]

BLAST of Pay0016163.1 vs. ExPASy TrEMBL
Match: A0A5B6ZC53 (ATP-dependent Clp protease proteolytic subunit OS=Davidia involucrata OX=16924 GN=Din_011085 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.6e-10
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 0


Query: 40  RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
           RIIRCGGA+DDDM+NIIVAQLLYLDAIDPN+DI+MYVNS
Sbjct: 120 RIIRCGGAVDDDMANIIVAQLLYLDAIDPNKDIVMYVNS 158

[Close]

BLAST of Pay0016163.1 vs. ExPASy TrEMBL
Match: A0A5B6ZBE3 (ATP-dependent Clp protease proteolytic subunit OS=Davidia involucrata OX=16924 GN=Din_011088 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.6e-10
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 0


Query: 40  RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
           RIIRCGGA+DDDM+NIIVAQLLYLDAIDPN+DI+MYVNS
Sbjct: 124 RIIRCGGAVDDDMANIIVAQLLYLDAIDPNKDIVMYVNS 162

[Close]

BLAST of Pay0016163.1 vs. ExPASy TrEMBL
Match: A0A5B6ZBH3 (ATP-dependent Clp protease proteolytic subunit OS=Davidia involucrata OX=16924 GN=Din_011083 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.6e-10
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 0


Query: 40  RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
           RIIRCGGA+DDDM+NIIVAQLLYLDAIDPN+DI+MYVNS
Sbjct: 120 RIIRCGGAVDDDMANIIVAQLLYLDAIDPNKDIVMYVNS 158

[Close]

BLAST of Pay0016163.1 vs. NCBI nr
Match: XP_008450213.1 (PREDICTED: uncharacterized protein LOC103491874 [Cucumis melo])

HSP 1 Score: 88.2 bits (217), Expect = 4.9e-14
Identity = 49/69 (71.01%), Postives = 54/69 (78.26%), Query Frame = 0


Query: 12 NRSLPVLTPVASSTKSDSCWCRDANSNR--------RIIRCGGALDDDMSNIIVAQLLYL 71
          +R  P+ TPVASSTKSDS   RDANS+R        RIIRCGGALDDDM+NIIVAQLLYL
Sbjct: 23 HRMPPMPTPVASSTKSDSYCYRDANSSRVATSYATQRIIRCGGALDDDMANIIVAQLLYL 82

Query: 72 DAIDPNEDI 73
          DAIDPN+ I
Sbjct: 83 DAIDPNKKI 91

[Close]

BLAST of Pay0016163.1 vs. NCBI nr
Match: KAG4928663.1 (hypothetical protein JHK85_055149 [Glycine max] >KHN43450.1 ATP-dependent Clp protease proteolytic subunit 5, chloroplastic [Glycine soja])

HSP 1 Score: 76.3 bits (186), Expect = 1.9e-10
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 0


Query: 40 RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
          RI+RCGGA+DDDMSNIIVAQLLYLDAIDPN+DI+MYVNS
Sbjct: 43 RIVRCGGAVDDDMSNIIVAQLLYLDAIDPNKDIVMYVNS 81

[Close]

BLAST of Pay0016163.1 vs. NCBI nr
Match: KAG5084173.1 (hypothetical protein JHK84_054211 [Glycine max] >KAG5086948.1 hypothetical protein JHK82_054345 [Glycine max] >RZB49106.1 ATP-dependent Clp protease proteolytic subunit 5, chloroplastic isoform A [Glycine soja] >RZB49107.1 ATP-dependent Clp protease proteolytic subunit 5, chloroplastic isoform B [Glycine soja])

HSP 1 Score: 76.3 bits (186), Expect = 1.9e-10
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 0


Query: 40 RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
          RI+RCGGA+DDDMSNIIVAQLLYLDAIDPN+DI+MYVNS
Sbjct: 42 RIVRCGGAVDDDMSNIIVAQLLYLDAIDPNKDIVMYVNS 80

[Close]

BLAST of Pay0016163.1 vs. NCBI nr
Match: KAG4916693.1 (hypothetical protein JHK87_054250 [Glycine soja])

HSP 1 Score: 76.3 bits (186), Expect = 1.9e-10
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 0


Query: 40 RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
          RI+RCGGA+DDDMSNIIVAQLLYLDAIDPN+DI+MYVNS
Sbjct: 42 RIVRCGGAVDDDMSNIIVAQLLYLDAIDPNKDIVMYVNS 80

[Close]

BLAST of Pay0016163.1 vs. NCBI nr
Match: XP_030947084.1 (ATP-dependent Clp protease proteolytic subunit 5, chloroplastic [Quercus lobata])

HSP 1 Score: 75.5 bits (184), Expect = 3.3e-10
Identity = 34/39 (87.18%), Postives = 39/39 (100.00%), Query Frame = 0


Query: 40  RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
           RIIRCGGA+DDDM+NIIVAQLLYLDA+DPN+DI+MYVNS
Sbjct: 119 RIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDILMYVNS 157

[Close]

BLAST of Pay0016163.1 vs. TAIR 10
Match: AT1G02560.1 (nuclear encoded CLP protease 5 )

HSP 1 Score: 72.8 bits (177), Expect = 2.0e-13
Identity = 33/39 (84.62%), Postives = 38/39 (97.44%), Query Frame = 0


Query: 40  RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
           RIIRCGGA+DDDM+NIIVAQLLYLDA+DP +DI+MYVNS
Sbjct: 123 RIIRCGGAVDDDMANIIVAQLLYLDAVDPTKDIVMYVNS 161

[Close]

BLAST of Pay0016163.1 vs. TAIR 10
Match: AT5G23140.1 (nuclear-encoded CLP protease P7 )

HSP 1 Score: 44.7 bits (104), Expect = 5.8e-05
Identity = 20/39 (51.28%), Postives = 31/39 (79.49%), Query Frame = 0


Query: 40 RIIRCGGALDDDMSNIIVAQLLYLDAIDPNEDIMMYVNS 79
          RII   G ++DD S+++VAQLLYL++ +P++ I MY+NS
Sbjct: 57 RIICINGPINDDTSHVVVAQLLYLESENPSKPIHMYLNS 95

[Close]

BLAST of Pay0016163.1 vs. TAIR 10
Match: AT5G45390.1 (CLP protease P4 )

HSP 1 Score: 41.2 bits (95), Expect = 6.4e-04
Identity = 24/75 (32.00%), Postives = 40/75 (53.33%), Query Frame = 0


Query: 4   SPHLSRCLNRSLPVLTPVASSTKSDSCWCRDANSNRRIIRCGGALDDDMSNIIVAQLLYL 63
           SP   R  N S+ +     S+ +             RI+  G ++DD +++ I++QLL L
Sbjct: 52  SPSPLRFANASIEMSQTQESAIRGAESDVMGLLLRERIVFLGSSIDDFVADAIMSQLLLL 111

Query: 64  DAIDPNEDIMMYVNS 79
           DA DP +DI +++NS
Sbjct: 112 DAKDPKKDIKLFINS 126

The following BLAST results are available for this feature:

BLAST of Pay0016163.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S834	2.8e-12	84.62	ATP-dependent Clp protease proteolytic subunit 5, chloroplastic OS=Arabidopsis t...	[more]
B9M0Y1	1.3e-06	58.97	ATP-dependent Clp protease proteolytic subunit OS=Geobacter daltonii (strain DSM...	[more]
A5GFA0	2.3e-06	56.41	ATP-dependent Clp protease proteolytic subunit OS=Geobacter uraniireducens (stra...	[more]
B5EI27	5.1e-06	53.85	ATP-dependent Clp protease proteolytic subunit OS=Geobacter bemidjiensis (strain...	[more]
C6E2T0	5.1e-06	53.85	ATP-dependent Clp protease proteolytic subunit OS=Geobacter sp. (strain M21) OX=...	[more]

BLAST of Pay0016163.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BPQ7	2.4e-14	71.01	uncharacterized protein LOC103491874 OS=Cucumis melo OX=3656 GN=LOC103491874 PE=...	[more]
A0A445FJU8	9.3e-11	89.74	ATP-dependent Clp protease proteolytic subunit 5, chloroplastic isoform A OS=Gly...	[more]
A0A5B6ZC53	1.6e-10	89.74	ATP-dependent Clp protease proteolytic subunit OS=Davidia involucrata OX=16924 G...	[more]
A0A5B6ZBE3	1.6e-10	89.74	ATP-dependent Clp protease proteolytic subunit OS=Davidia involucrata OX=16924 G...	[more]
A0A5B6ZBH3	1.6e-10	89.74	ATP-dependent Clp protease proteolytic subunit OS=Davidia involucrata OX=16924 G...	[more]

BLAST of Pay0016163.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008450213.1	4.9e-14	71.01	PREDICTED: uncharacterized protein LOC103491874 [Cucumis melo]	[more]
KAG4928663.1	1.9e-10	89.74	hypothetical protein JHK85_055149 [Glycine max] >KHN43450.1 ATP-dependent Clp pr...	[more]
KAG5084173.1	1.9e-10	89.74	hypothetical protein JHK84_054211 [Glycine max] >KAG5086948.1 hypothetical prote...	[more]
KAG4916693.1	1.9e-10	89.74	hypothetical protein JHK87_054250 [Glycine soja]	[more]
XP_030947084.1	3.3e-10	87.18	ATP-dependent Clp protease proteolytic subunit 5, chloroplastic [Quercus lobata]	[more]

BLAST of Pay0016163.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02560.1	2.0e-13	84.62	nuclear encoded CLP protease 5	[more]
AT5G23140.1	5.8e-05	51.28	nuclear-encoded CLP protease P7	[more]
AT5G45390.1	6.4e-04	32.00	CLP protease P4	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023562	Clp protease proteolytic subunit /Translocation-enhancing protein TepA	PFAM	PF00574	CLP_protease	coord: 38..79 e-value: 2.8E-9 score: 37.1
IPR023562	Clp protease proteolytic subunit /Translocation-enhancing protein TepA	PANTHER	PTHR10381	ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT	coord: 38..98
None	No IPR available	GENE3D	3.90.226.10		coord: 17..79 e-value: 2.6E-10 score: 42.1
None	No IPR available	PANTHER	PTHR10381:SF12	ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT 5, CHLOROPLASTIC	coord: 38..98
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 32..78

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Pay0016163	Pay0016163	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Pay0016163.1-exon	Pay0016163.1-exon-chr09:14235865..14235993	exon
Pay0016163.1-exon	Pay0016163.1-exon-chr09:14236097..14236189	exon
Pay0016163.1-exon	Pay0016163.1-exon-chr09:14236453..14236569	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Pay0016163.1-cds	Pay0016163.1-cds-chr09:14235865..14235993	CDS
Pay0016163.1-cds	Pay0016163.1-cds-chr09:14236097..14236189	CDS
Pay0016163.1-cds	Pay0016163.1-cds-chr09:14236453..14236569	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Pay0016163.1	Pay0016163.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009526	plastid envelope
molecular_function	GO:0004176	ATP-dependent peptidase activity
molecular_function	GO:0004252	serine-type endopeptidase activity