Pay0014327.1 (mRNA) Melon (Payzawat) v1

Overview


Name	Pay0014327.1
Type	mRNA
Organism	Cucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	chr01: 16779010 .. 16779928 (+)
Sequence length	414
RNA-Seq Expression	Pay0014327.1
Synteny	Pay0014327.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTAATAACTGATACTAAGTTTTTCCTCTCGTCACACTTTGAAATGAAAGACCTGGGAGAAGCAGACGTAATCCTAGGTGTTAAAATCAGGAAAAACAAAACCAGTTTGTCTCTATGTCAATCTCATTACGTAGAGAAAATACTAAAGAAGTTTGATTCCTTTGATGTTTCTCCTGTGAGAACTCCCTTTGACGCTAGTAAACATCTTAAGAAGAATAAAGGAGATAGTGTGTCTCAACCCGAATATGCAAAGATCATAGGTAGTGTGATGTATTTAATGAATTACACTAGACCAGATATTGCATATGCTGTCAGTAGATTAAGTAGATATACACACAATCCTAATAGATACCACTGGGATGCCTACGCCATCTGTTGAGATATCTTAAAGGGACGATAGATTACTGTCTACACTTCAACAAATTTCCTGCCGTACTAGAAGGATATTGTGATGCAAACTGGGTCACAGATAATGATGAGGTTAACTCTACTAGTGGGTATGTATTTTTACTCGGAGGTGGAGCTATATCTTGGAAGTCTACAAAACAGACTTGTATAGCCAGATCCACGATGGAATCCGAGTTCATAGCACTAGAGTTGGCAGGACAGAGGCTGAGTGGATCAAAAATCTACTAGGAGATGTACCATTGTGGAGGACATCTGTACCGGTGTCCATACAGTGTGATTCGCAAGCCGCGATATGTACTGCCAAGAACAGTGTTTATAATGGTAAAAGTAGACACATACGTCTTAGGCATGCAGTAGTAAAACAACTGCTAAAGGAAGGAACCATCTCCTTGGAATTTGTTCGATCTGAGAAGAACTTGGTTGATCCTTTAACCAAAGGACTAACAAGAAAAATGGTATTAGATTCCTCTGTGAACATGGGCCTGAAGCCCTTCAGAGATCCATAG

mRNA sequence

ATGGAGTTAATAACTGATACTAAGTTTTTCCTCTCGTCACACTTTGAAATGAAAGACCTGGGAGAAGCAGACGTAATCCTAGGTGTTAAAATCAGGAAAAACAAAACCAGTTTGTCTCTATGTCAATCTCATTACGTAGAGAAAATACTAAAGAAGTTTGATTCCTTTGATGTTTCTCCTTGTGATTCGCAAGCCGCGATATGTACTGCCAAGAACAGTGTTTATAATGGTAAAAGTAGACACATACGTCTTAGGCATGCAGTAGTAAAACAACTGCTAAAGGAAGGAACCATCTCCTTGGAATTTGTTCGATCTGAGAAGAACTTGGTTGATCCTTTAACCAAAGGACTAACAAGAAAAATGGTATTAGATTCCTCTGTGAACATGGGCCTGAAGCCCTTCAGAGATCCATAG

Coding sequence (CDS)

ATGGAGTTAATAACTGATACTAAGTTTTTCCTCTCGTCACACTTTGAAATGAAAGACCTGGGAGAAGCAGACGTAATCCTAGGTGTTAAAATCAGGAAAAACAAAACCAGTTTGTCTCTATGTCAATCTCATTACGTAGAGAAAATACTAAAGAAGTTTGATTCCTTTGATGTTTCTCCTTGTGATTCGCAAGCCGCGATATGTACTGCCAAGAACAGTGTTTATAATGGTAAAAGTAGACACATACGTCTTAGGCATGCAGTAGTAAAACAACTGCTAAAGGAAGGAACCATCTCCTTGGAATTTGTTCGATCTGAGAAGAACTTGGTTGATCCTTTAACCAAAGGACTAACAAGAAAAATGGTATTAGATTCCTCTGTGAACATGGGCCTGAAGCCCTTCAGAGATCCATAG

Protein sequence

MELITDTKFFLSSHFEMKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSPCDSQAAICTAKNSVYNGKSRHIRLRHAVVKQLLKEGTISLEFVRSEKNLVDPLTKGLTRKMVLDSSVNMGLKPFRDP

Homology

[Close]

BLAST of Pay0014327.1 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 4.1e-05
Identity = 28/66 (42.42%), Postives = 40/66 (60.61%), Query Frame = 0


Query: 3    LITDTKFFLSSHFEMKDLGEADVILGVKIRKNKTS--LSLCQSHYVEKILKKFDSFDVSP 62
            LI   K  LS  F+MKDLG A  ILG+KI + +TS  L L Q  Y+E++L++F+  +  P
Sbjct: 1021 LIAKLKGDLSKSFDMKDLGPAQQILGMKIVRERTSRKLWLSQEKYIERVLERFNMKNAKP 1080

Query: 63   CDSQAA 67
              +  A
Sbjct: 1081 VSTPLA 1086

HSP 2 Score: 45.1 bits (105), Expect = 7.6e-04
Identity = 19/59 (32.20%), Postives = 41/59 (69.49%), Query Frame = 0


Query: 61   CDSQAAICTAKNSVYNGKSRHIRLRHAVVKQLLKEGTISLEFVRSEKNLVDPLTKGLTR 120
            CDSQ+AI  +KNS+Y+ +++HI +R+  +++++ + ++ +  + + +N  D LTK + R
Sbjct: 1255 CDSQSAIDLSKNSMYHARTKHIDVRYHWIREMVDDESLKVLKISTNENPADMLTKVVPR 1313

[Close]

BLAST of Pay0014327.1 vs. ExPASy TrEMBL
Match: A0A1S3B571 (uncharacterized protein LOC103485933 OS=Cucumis melo OX=3656 GN=LOC103485933 PE=4 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 4.1e-61
Identity = 122/137 (89.05%), Postives = 128/137 (93.43%), Query Frame = 0


Query: 1   MELITDTKFFLSSHFEMKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP 60
           MELITDTK FLSSHFEMKDLGEADVILGVK RKNKTSLSLCQSHYVEKILKKFDSFDVSP
Sbjct: 1   MELITDTKLFLSSHFEMKDLGEADVILGVKNRKNKTSLSLCQSHYVEKILKKFDSFDVSP 60

Query: 61  CDSQAAICTAKNSVYNGKSRHIRLRHAVVKQLLKEGTISLEFVRSEKNLVDPLTKGLTRK 120
            DSQ AICT KN+VYNGKSRHIRLRHA+VKQLLKE TIS+EFVRSEKNL +PLTKGLTRK
Sbjct: 61  YDSQDAICTTKNNVYNGKSRHIRLRHAIVKQLLKEETISMEFVRSEKNLANPLTKGLTRK 120

Query: 121 MVLDSSVNMGLKPFRDP 138
           +VLDS +NMGLKPF DP
Sbjct: 121 VVLDSPMNMGLKPFEDP 137

[Close]

BLAST of Pay0014327.1 vs. ExPASy TrEMBL
Match: A0A5A7TXL0 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G001310 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.2e-47
Identity = 114/179 (63.69%), Postives = 117/179 (65.36%), Query Frame = 0


Query: 17  MKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP---------------- 76
           MKDLGEAD+ILGVKIRKNKTSLSL QSHYVEKIL+KF+SFDVSP                
Sbjct: 1   MKDLGEADIILGVKIRKNKTSLSLYQSHYVEKILRKFESFDVSPVRTPFDASKHLKKNKG 60

Query: 77  ------------------------------------------CDSQAAICTAKNSVYNGK 136
                                                     CDSQAAICTAKNSVYNGK
Sbjct: 61  DSVSQPEYAKIIGTIDYCLHFNKFPAVLEGYCDANWVTDNDECDSQAAICTAKNSVYNGK 120

Query: 137 SRHIRLRHAVVKQLLKEGTISLEFVRSEKNLVDPLTKGLTRKMVLDSSVNMGLKPFRDP 138
           SRHIRLRHAVVKQLLKEGTISLEFVRSEKNL DPLTKGLTRKMVLDSSVNMGLKPF  P
Sbjct: 121 SRHIRLRHAVVKQLLKEGTISLEFVRSEKNLADPLTKGLTRKMVLDSSVNMGLKPFGYP 179

[Close]

BLAST of Pay0014327.1 vs. ExPASy TrEMBL
Match: A0A5D3C5T2 (Ty1-copia retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold70G00500 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 4.4e-47
Identity = 133/306 (43.46%), Postives = 135/306 (44.12%), Query Frame = 0


Query: 1    MELITDTKFFLSSHFEMKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP 60
            MELITDTK+FLSSHFEMKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP
Sbjct: 1041 MELITDTKYFLSSHFEMKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP 1100

Query: 61   ------------------------------------------------------------ 120
                                                                        
Sbjct: 1101 VRTPFDASKHLKKNKGDSVSQPEYAKIIGSVMYLMNYTRPDIAYAVSRLSRYTHNPNRYH 1160

Query: 121  ------------------------------------------------------------ 138
                                                                        
Sbjct: 1161 WDALRHLLRYLKGTIDYCLHFKKFPAVLEGYCDANWVTDNDEVNSTSGYVFLLGGGAISW 1220

[Close]

BLAST of Pay0014327.1 vs. ExPASy TrEMBL
Match: A0A5A7UYD5 (Ty1-copia retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold132G00940 PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 4.9e-46
Identity = 114/196 (58.16%), Postives = 116/196 (59.18%), Query Frame = 0


Query: 20  LGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP------------------- 79
           LGEADVILGVKIRKNKTSLSLCQSHYVEKILKKF+SFDVSP                   
Sbjct: 739 LGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFNSFDVSPVKTPFDARKHLKKNKGDSV 798

Query: 80  -----------------------------------------------------------C 138
                                                                      C
Sbjct: 799 SQPEYAKIIGTIDYCLHFNKFSAVLEGFCDANWVTNNDEVNSTGGRCTIVGDIYTGVHKC 858

[Close]

BLAST of Pay0014327.1 vs. ExPASy TrEMBL
Match: A0A5D3CMJ4 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold828G00100 PE=4 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 8.4e-46
Identity = 115/207 (55.56%), Postives = 116/207 (56.04%), Query Frame = 0


Query: 17  MKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP---------------- 76
           MKDLGEAD+ILGVKIRKNK SLSLCQSHYVEKILKKFD FDVSP                
Sbjct: 1   MKDLGEADIILGVKIRKNKASLSLCQSHYVEKILKKFDFFDVSPARTSFDASKHLKKNKR 60

Query: 77  ------------------------------------------------------------ 136
                                                                       
Sbjct: 61  DSVSQSEYAKIIGYCDANWVTDNDEVNSTSGSTMESEFIALELAGQEAEWIKNLLGDVPL 120

Query: 137 ----------CDSQAAICTAKNSVYNGKSRHIRLRHAVVKQLLKEGTISLEFVRSEKNLV 138
                     CDSQAAICTAKNSVYNGKSRHIRLRH VVKQLLKEGTISLEFVRSEKNL 
Sbjct: 121 WGTFVPVSIQCDSQAAICTAKNSVYNGKSRHIRLRHVVVKQLLKEGTISLEFVRSEKNLA 180

[Close]

BLAST of Pay0014327.1 vs. NCBI nr
Match: XP_008441939.1 (PREDICTED: uncharacterized protein LOC103485933 [Cucumis melo])

HSP 1 Score: 243.8 bits (621), Expect = 8.6e-61
Identity = 122/137 (89.05%), Postives = 128/137 (93.43%), Query Frame = 0


Query: 1   MELITDTKFFLSSHFEMKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP 60
           MELITDTK FLSSHFEMKDLGEADVILGVK RKNKTSLSLCQSHYVEKILKKFDSFDVSP
Sbjct: 1   MELITDTKLFLSSHFEMKDLGEADVILGVKNRKNKTSLSLCQSHYVEKILKKFDSFDVSP 60

Query: 61  CDSQAAICTAKNSVYNGKSRHIRLRHAVVKQLLKEGTISLEFVRSEKNLVDPLTKGLTRK 120
            DSQ AICT KN+VYNGKSRHIRLRHA+VKQLLKE TIS+EFVRSEKNL +PLTKGLTRK
Sbjct: 61  YDSQDAICTTKNNVYNGKSRHIRLRHAIVKQLLKEETISMEFVRSEKNLANPLTKGLTRK 120

Query: 121 MVLDSSVNMGLKPFRDP 138
           +VLDS +NMGLKPF DP
Sbjct: 121 VVLDSPMNMGLKPFEDP 137

[Close]

BLAST of Pay0014327.1 vs. NCBI nr
Match: KAA0048262.1 (hypothetical protein E6C27_scaffold63G001830 [Cucumis melo var. makuwa] >TYK07994.1 hypothetical protein E5676_scaffold265G001310 [Cucumis melo var. makuwa])

HSP 1 Score: 199.1 bits (505), Expect = 2.4e-47
Identity = 114/179 (63.69%), Postives = 117/179 (65.36%), Query Frame = 0


Query: 17  MKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP---------------- 76
           MKDLGEAD+ILGVKIRKNKTSLSL QSHYVEKIL+KF+SFDVSP                
Sbjct: 1   MKDLGEADIILGVKIRKNKTSLSLYQSHYVEKILRKFESFDVSPVRTPFDASKHLKKNKG 60

Query: 77  ------------------------------------------CDSQAAICTAKNSVYNGK 136
                                                     CDSQAAICTAKNSVYNGK
Sbjct: 61  DSVSQPEYAKIIGTIDYCLHFNKFPAVLEGYCDANWVTDNDECDSQAAICTAKNSVYNGK 120

Query: 137 SRHIRLRHAVVKQLLKEGTISLEFVRSEKNLVDPLTKGLTRKMVLDSSVNMGLKPFRDP 138
           SRHIRLRHAVVKQLLKEGTISLEFVRSEKNL DPLTKGLTRKMVLDSSVNMGLKPF  P
Sbjct: 121 SRHIRLRHAVVKQLLKEGTISLEFVRSEKNLADPLTKGLTRKMVLDSSVNMGLKPFGYP 179

[Close]

BLAST of Pay0014327.1 vs. NCBI nr
Match: TYK06518.1 (ty1-copia retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 197.2 bits (500), Expect = 9.2e-47
Identity = 133/306 (43.46%), Postives = 135/306 (44.12%), Query Frame = 0


Query: 1    MELITDTKFFLSSHFEMKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP 60
            MELITDTK+FLSSHFEMKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP
Sbjct: 1041 MELITDTKYFLSSHFEMKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP 1100

Query: 61   ------------------------------------------------------------ 120
                                                                        
Sbjct: 1101 VRTPFDASKHLKKNKGDSVSQPEYAKIIGSVMYLMNYTRPDIAYAVSRLSRYTHNPNRYH 1160

Query: 121  ------------------------------------------------------------ 138
                                                                        
Sbjct: 1161 WDALRHLLRYLKGTIDYCLHFKKFPAVLEGYCDANWVTDNDEVNSTSGYVFLLGGGAISW 1220

[Close]

BLAST of Pay0014327.1 vs. NCBI nr
Match: KAA0058501.1 (ty1-copia retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 193.7 bits (491), Expect = 1.0e-45
Identity = 114/196 (58.16%), Postives = 116/196 (59.18%), Query Frame = 0


Query: 20  LGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP------------------- 79
           LGEADVILGVKIRKNKTSLSLCQSHYVEKILKKF+SFDVSP                   
Sbjct: 739 LGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFNSFDVSPVKTPFDARKHLKKNKGDSV 798

Query: 80  -----------------------------------------------------------C 138
                                                                      C
Sbjct: 799 SQPEYAKIIGTIDYCLHFNKFSAVLEGFCDANWVTNNDEVNSTGGRCTIVGDIYTGVHKC 858

[Close]

BLAST of Pay0014327.1 vs. NCBI nr
Match: KAA0063384.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa] >TYK11646.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 193.0 bits (489), Expect = 1.7e-45
Identity = 115/207 (55.56%), Postives = 116/207 (56.04%), Query Frame = 0


Query: 17  MKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSP---------------- 76
           MKDLGEAD+ILGVKIRKNK SLSLCQSHYVEKILKKFD FDVSP                
Sbjct: 1   MKDLGEADIILGVKIRKNKASLSLCQSHYVEKILKKFDFFDVSPARTSFDASKHLKKNKR 60

Query: 77  ------------------------------------------------------------ 136
                                                                       
Sbjct: 61  DSVSQSEYAKIIGYCDANWVTDNDEVNSTSGSTMESEFIALELAGQEAEWIKNLLGDVPL 120

Query: 137 ----------CDSQAAICTAKNSVYNGKSRHIRLRHAVVKQLLKEGTISLEFVRSEKNLV 138
                     CDSQAAICTAKNSVYNGKSRHIRLRH VVKQLLKEGTISLEFVRSEKNL 
Sbjct: 121 WGTFVPVSIQCDSQAAICTAKNSVYNGKSRHIRLRHVVVKQLLKEGTISLEFVRSEKNLA 180

[Close]

BLAST of Pay0014327.1 vs. TAIR 10
Match: ATMG00810.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 1.2e-04
Identity = 25/69 (36.23%), Postives = 36/69 (52.17%), Query Frame = 0


Query: 9  FFLSSHFEMKDLGEADVILGVKIRKNKTSLSLCQSHYVEKILKKFDSFDVSPCDS----- 68
          F LSS F MKDLG     LG++I+ + + L L Q+ Y E+IL      D  P  +     
Sbjct: 25 FQLSSTFSMKDLGPVHYFLGIQIKTHPSGLFLSQTKYAEQILNNAGMLDCKPMSTPLPLK 84

Query: 69 -QAAICTAK 72
            +++ TAK
Sbjct: 85 LNSSVSTAK 93

The following BLAST results are available for this feature:

BLAST of Pay0014327.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10978	4.1e-05	42.42	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]

BLAST of Pay0014327.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B571	4.1e-61	89.05	uncharacterized protein LOC103485933 OS=Cucumis melo OX=3656 GN=LOC103485933 PE=...	[more]
A0A5A7TXL0	1.2e-47	63.69	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5D3C5T2	4.4e-47	43.46	Ty1-copia retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A5A7UYD5	4.9e-46	58.16	Ty1-copia retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A5D3CMJ4	8.4e-46	55.56	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var....	[more]

BLAST of Pay0014327.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008441939.1	8.6e-61	89.05	PREDICTED: uncharacterized protein LOC103485933 [Cucumis melo]	[more]
KAA0048262.1	2.4e-47	63.69	hypothetical protein E6C27_scaffold63G001830 [Cucumis melo var. makuwa] >TYK0799...	[more]
TYK06518.1	9.2e-47	43.46	ty1-copia retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0058501.1	1.0e-45	58.16	ty1-copia retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0063384.1	1.7e-45	55.56	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m...	[more]

BLAST of Pay0014327.1 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00810.1	1.2e-04	36.23	DNA/RNA polymerases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013103	Reverse transcriptase, RNA-dependent DNA polymerase	PFAM	PF07727	RVT_2	coord: 1..61 e-value: 3.1E-9 score: 36.8
None	No IPR available	CDD	cd09272	RNase_HI_RT_Ty1	coord: 61..120 e-value: 2.63154E-21 score: 81.3605

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Pay0014327	Pay0014327	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Pay0014327.1-exon	Pay0014327.1-exon-chr01:16779010..16779189	exon
Pay0014327.1-exon	Pay0014327.1-exon-chr01:16779695..16779928	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Pay0014327.1-cds	Pay0014327.1-cds-chr01:16779010..16779189	CDS
Pay0014327.1-cds	Pay0014327.1-cds-chr01:16779695..16779928	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Pay0014327.1	Pay0014327.1-protein	polypeptide