Pay0005923.1 (mRNA) Melon (Payzawat) v1

BLAST of Pay0005923.1 vs. ExPASy Swiss-Prot
Match: O22494 (Glutathione synthetase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=GSH2 PE=2 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 3.5e-209
Identity = 371/558 (66.49%), Postives = 441/558 (79.03%), Query Frame = 0

Query: 1   MGSSHSSCSLT-PTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFK-----LQPFLFSSS 60
           MGS  SS S++  T +T HF              +Q  + NS+NF      L+P L  SS
Sbjct: 1   MGSGCSSPSISLTTIATSHF-------------QSQESLSNSLNFYSPTRFLEPHLLKSS 60

Query: 61  S-SSQVLPLKCRKAAEFETQAK-SLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDR 120
                  PLKC K  E +TQ + S K + D H ID +L+Q++  DALVW  L GL+VGDR
Sbjct: 61  KIFIPKSPLKCAKVPEMQTQLEDSAKPIVDPHDIDSKLVQKLANDALVWCPLRGLLVGDR 120

Query: 121 SVKRSGEVPGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSR 180
           + +RSG +PGV + H+P+AL+P  FPE+HW QACE++ IFNELVDRVS DG+FLQ+SLSR
Sbjct: 121 NSERSGTIPGVDMVHAPVALIPMSFPESHWKQACEVAPIFNELVDRVSQDGEFLQQSLSR 180

Query: 181 TKKGDAFTSRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGL 240
           T+K D FTSRLL+IHSKML+I+K EEIRLGLHRSDYMLD++TKLLLQ+ELNTIS SFPGL
Sbjct: 181 TRKADPFTSRLLEIHSKMLEINKLEEIRLGLHRSDYMLDEQTKLLLQIELNTISSSFPGL 240

Query: 241 SCLVSELHRFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVIQAE 300
           SCLVSELHR LL +Y E +  D   IP N A NQ+AE LAKAW EY DP AVI+FV+QAE
Sbjct: 241 SCLVSELHRSLLQQYREDIASDPNRIPANNAVNQFAEALAKAWNEYGDPRAVIIFVVQAE 300

Query: 301 ERNMYDQLWLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVLDGQAIAVVYFRAGYTP 360
           ERNMYDQ WLS +LR+RH + T+RKTLAEID  G L+ DGTLV+DGQA+AV+YFRAGY P
Sbjct: 301 ERNMYDQHWLSASLRERHQVTTIRKTLAEIDALGELQQDGTLVVDGQAVAVIYFRAGYAP 360

Query: 361 RDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIR 420
            DY SESEW ARLLMEQS AVKCPSISYHL G+KKIQQELA+PNVLERFL++++DIAK+R
Sbjct: 361 SDYHSESEWKARLLMEQSRAVKCPSISYHLAGSKKIQQELAKPNVLERFLENKDDIAKLR 420

Query: 421 KCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGA 480
           KCFAGLWSLD+ DIVK AIE PEL+VMKPQREGGGNNIYG+DVR ALLKLQ+EG+  D A
Sbjct: 421 KCFAGLWSLDESDIVKDAIERPELYVMKPQREGGGNNIYGEDVRGALLKLQKEGTGSDAA 480

Query: 481 YILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSS 540
           YILMQRIFP +  +I +R+GI HK+  +SELGI+G YLRNK +V++NQQ GYLMRTKVSS
Sbjct: 481 YILMQRIFPKISHSILMREGISHKEETISELGIYGTYLRNKTEVLINQQAGYLMRTKVSS 540

Query: 541 SNEGGVAAGFAVLDSIYL 551
           S+EGGVAAGFAVLDSIYL
Sbjct: 541 SDEGGVAAGFAVLDSIYL 545

BLAST of Pay0005923.1 vs. ExPASy Swiss-Prot
Match: O23732 (Glutathione synthetase, chloroplastic OS=Brassica juncea OX=3707 GN=GSH2 PE=2 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 1.5e-204
Identity = 341/513 (66.47%), Postives = 427/513 (83.24%), Query Frame = 0

Query: 38  IRNSINFKLQPFLFSSSSSSQVLPLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYD 97
           + +S+    Q F+F  +   +  PL+C  +   E    S K + D    D   +Q++VYD
Sbjct: 22  LSSSLKLNPQSFIFHLNLRKRP-PLRCLSSLTME----SPKPILDFEKFDDGFVQKLVYD 81

Query: 98  ALVWSSLHGLVVGDRSVKRSGEVPGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVD 157
           ALVWSSLHGLVVGD++ +RSG VPGVG+ H+PIALLP  FPE++WNQACE++ IFNELVD
Sbjct: 82  ALVWSSLHGLVVGDKTHQRSGTVPGVGMMHAPIALLPTPFPESYWNQACEVAPIFNELVD 141

Query: 158 RVSMDGKFLQESLSRTKKGDAFTSRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLL 217
           R+S+DGKF+Q+SLSRT+K D FTSRLL+IHSKML+ +K+EEIRLGLHRSDYMLD++TK L
Sbjct: 142 RISLDGKFIQDSLSRTRKADVFTSRLLEIHSKMLESNKREEIRLGLHRSDYMLDEETKSL 201

Query: 218 LQVELNTISCSFPGLSCLVSELHRFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTE 277
           LQ+E+NTISCSFPG   LV+ELH+ LL  +G+ LGLD K +P N + +Q+A+ +AKAW E
Sbjct: 202 LQIEMNTISCSFPGFGRLVTELHQSLLRSHGDHLGLDSKRVPRNGSTSQFADAMAKAWLE 261

Query: 278 YNDPSAVILFVIQAEERNMYDQLWLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVLD 337
           YN+P AV++ V+Q +ERNMYDQ WLS  LR++HNI T+RK+LAE++ EG +  DGTL + 
Sbjct: 262 YNNPRAVVMVVVQPDERNMYDQHWLSSVLREKHNIVTIRKSLAEVETEGRVHEDGTLTVG 321

Query: 338 GQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNV 397
           GQA++VVY+R+GYTPRDYPSESEW+ARLL+EQSSAVKCPSI+YHL GTKKIQQELA+P V
Sbjct: 322 GQAVSVVYYRSGYTPRDYPSESEWNARLLIEQSSAVKCPSIAYHLAGTKKIQQELAKPGV 381

Query: 398 LERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVRE 457
           LERF+D+++DIAK+RKCFAGLWSLDD +I+KKAIE PELFVMKPQREGGGNNIYGDDVRE
Sbjct: 382 LERFMDNKDDIAKLRKCFAGLWSLDDPEIIKKAIEKPELFVMKPQREGGGNNIYGDDVRE 441

Query: 458 ALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVI 517
            LL+LQ EG EE+ AYILMQRIFP V     +RDG+ HKD A+SELG++GAYLRNK++VI
Sbjct: 442 NLLRLQREGEEENAAYILMQRIFPKVSNVFLLRDGVYHKDQAISELGVYGAYLRNKERVI 501

Query: 518 VNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYL 551
           +N+ +GYLMRTKVSSS+EGGVAAG+AVLDSIYL
Sbjct: 502 INEHSGYLMRTKVSSSDEGGVAAGYAVLDSIYL 529

BLAST of Pay0005923.1 vs. ExPASy Swiss-Prot
Match: P46416 (Glutathione synthetase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GSH2 PE=2 SV=3)

HSP 1 Score: 697.2 bits (1798), Expect = 1.5e-199
Identity = 350/553 (63.29%), Postives = 442/553 (79.93%), Query Frame = 0

Query: 1   MGSSHSSCSLTPTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFKLQP--FLFSSSSS-S 60
           MGS  SS S + + +     FS S  +PS          +S + KL P  FLF +  +  
Sbjct: 1   MGSGCSSLSYSSSSTCNATVFSISS-SPS----------SSSSLKLNPSSFLFQNPKTLR 60

Query: 61  QVLPLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRS 120
              PL+C ++ + E+Q    K + D+  +D E +Q++VYDALVWSSLHGLVVGD+S ++S
Sbjct: 61  NQSPLRCGRSFKMESQ----KPIFDLEKLDDEFVQKLVYDALVWSSLHGLVVGDKSYQKS 120

Query: 121 GEVPGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGD 180
           G VPGVGL H+PIALLP  FPE +W QAC ++ +FNEL+DRVS+DGKFLQ+SLSRTKK D
Sbjct: 121 GNVPGVGLMHAPIALLPTAFPEAYWKQACNVTPLFNELIDRVSLDGKFLQDSLSRTKKVD 180

Query: 181 AFTSRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVS 240
            FTSRLLDIHSKML+ +KKE+IRLGLHR DYMLD++T  LLQ+E+NTISCSFPGLS LVS
Sbjct: 181 VFTSRLLDIHSKMLERNKKEDIRLGLHRFDYMLDEETNSLLQIEMNTISCSFPGLSRLVS 240

Query: 241 ELHRFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVIQAEERNMY 300
           +LH+ LL  YG+ +G+D + +P N +  Q+A+ LAKAW EY++P AV++ ++Q EERNMY
Sbjct: 241 QLHQSLLRSYGDQIGIDSERVPINTSTIQFADALAKAWLEYSNPRAVVMVIVQPEERNMY 300

Query: 301 DQLWLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVLDGQAIAVVYFRAGYTPRDYPS 360
           DQ  LS  LR++HNI  +RKTLAE+++EG ++ D TL++ GQA+AVVYFR+GYTP D+PS
Sbjct: 301 DQHLLSSILREKHNIVVIRKTLAEVEKEGSVQEDETLIVGGQAVAVVYFRSGYTPNDHPS 360

Query: 361 ESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAG 420
           ESEW+ARLL+E+SSAVKCPSI+YHL G+KKIQQELA+P VLERFLD++EDIAK+RKCFAG
Sbjct: 361 ESEWNARLLIEESSAVKCPSIAYHLTGSKKIQQELAKPGVLERFLDNKEDIAKLRKCFAG 420

Query: 421 LWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQ 480
           LWSLDD +IVK+AIE P LFVMKPQREGGGNNIYGDDVRE LL+LQ+EG E + AYILMQ
Sbjct: 421 LWSLDDSEIVKQAIEKPGLFVMKPQREGGGNNIYGDDVRENLLRLQKEGEEGNAAYILMQ 480

Query: 481 RIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGG 540
           RIFP V     VR+G+ HK  A+SELG++GAYLR+KD+VIVN+Q+GYLMRTK++SS+EGG
Sbjct: 481 RIFPKVSNMFLVREGVYHKHQAISELGVYGAYLRSKDEVIVNEQSGYLMRTKIASSDEGG 538

Query: 541 VAAGFAVLDSIYL 551
           VAAGF VLDSIYL
Sbjct: 541 VAAGFGVLDSIYL 538

BLAST of Pay0005923.1 vs. ExPASy Swiss-Prot
Match: P35668 (Glutathione synthetase OS=Xenopus laevis OX=8355 GN=gss PE=2 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 7.6e-95
Identity = 199/474 (41.98%), Postives = 293/474 (61.81%), Query Frame = 0

Query: 87  DQELLQEMVYDALVWSSLHGLVVGDRSVKRSGEVPGVGLSHSPIALLPGLFPETHWNQAC 146
           D +LL+E+   A+  + L G+++  +    S +V    +S +P ALLP   P+  + QA 
Sbjct: 11  DTKLLEELAPIAIDAALLQGVLMRTKESPNSSDV----VSFAPFALLPSPVPKALFEQAK 70

Query: 147 ELSSIFNELVDRVSMDGKFLQESLSRTKKGDAFTSRLLDIHSKMLQIDKKEEIRLGLHRS 206
            +   FN LVDR+S D  FL++ LS T K D F  RL  IH ++ Q D  +E+ LG++RS
Sbjct: 71  CVQEDFNTLVDRISQDTSFLEQVLSSTIKVDDFIRRLFAIHKQVQQEDCTQEVFLGINRS 130

Query: 207 DYML---DDKTKLLLQVELNTISCSFPGLSCLVSELHRFLLGEYGEVLGLDYKNIPENYA 266
           DYM    DD T  L Q+E+NTI+ SF GL+     +H+ +L    +    +  +I  N A
Sbjct: 131 DYMFDCRDDGTPALKQIEINTIAASFGGLASRTPAVHQHVLKFLRK--SEESSSILTNDA 190

Query: 267 ANQYAEVLAKAWTEYNDPSAVILFVIQAEERNMYDQLWLSVNLRKRHNIKTLRKTLAEID 326
                  +A AW  Y    A ++F+++ E+RN+ DQ ++   L KR N++ +R+ LA++ 
Sbjct: 191 VEGIGWGIAHAWALYGSVDATVMFLVENEQRNILDQRFIEAELCKR-NVRVIRRRLADVF 250

Query: 327 REGVLRSDGTLVLDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLV 386
             G L  +  L +DG  +AV YFR GY P+DY +E +W ARL++E+S AVKCP +   LV
Sbjct: 251 ERGTLDEERHLFIDGYEVAVAYFRTGYVPQDY-TEQDWEARLMLERSRAVKCPDVPTQLV 310

Query: 387 GTKKIQQELARPNVLERFL-DSEEDIAKIRKCFAGLWSLD----DKDIVKKAIETPELFV 446
           GTKK+QQEL+RP +LE+FL D  E +A+IR+ F GL+SLD      + V+ A+  P+ FV
Sbjct: 311 GTKKVQQELSRPQILEKFLPDKPEAVARIRETFTGLYSLDIGEEGDEAVRVALANPDQFV 370

Query: 447 MKPQREGGGNNIYGDDVREALLKLQE-EGSEEDGAYILMQRIFPSVFPTIFVR-DGICHK 506
           +KPQREGGGNN+YG++++E   KLQE + SEE  +YILM +I P       +R  G    
Sbjct: 371 LKPQREGGGNNLYGEELKE---KLQECKDSEERTSYILMDKINPKPLKNCLLRAGGRVQI 430

Query: 507 DHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYL 551
              +SELG+FG Y+R++D++I   Q G+L+RTK    ++GGVAAG AVLD+ YL
Sbjct: 431 SECISELGMFGVYVRHRDQMIYYDQVGHLLRTKAIEHSDGGVAAGVAVLDNPYL 473

BLAST of Pay0005923.1 vs. ExPASy Swiss-Prot
Match: Q54E83 (Glutathione synthetase OS=Dictyostelium discoideum OX=44689 GN=gshB PE=3 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.2e-92
Identity = 198/476 (41.60%), Postives = 295/476 (61.97%), Query Frame = 0

Query: 88  QELLQEMVYDALVWSSLHGLVVGDRSVKRSGEVPGVGLSHSPIALLPGLFPETHWNQACE 147
           +E L ++    + W+  +GL++  +  +   +   V ++H P +L P    +  +N+AC+
Sbjct: 2   EEKLNDLKEQGIDWAFANGLIMIKKPTEEEAKNNVVNVTHVPFSLYPSKMNKKLFNEACK 61

Query: 148 LSSIFNELVDRVSMDGKFLQESLSRTKKGDAFTSRLLDIHSKMLQIDKKEEIRLGLHRSD 207
           L+  +N LV  +S D  FLQ +L      D FT  LL+I  K+++   K++I LG+ RSD
Sbjct: 62  LAEDYNLLVHNISKDYDFLQNTLKDV--FDDFTQMLLNIQRKVVKEGIKQKISLGIFRSD 121

Query: 208 YMLDDK----TKLLLQVELNTISCSFPGLSCLVSELHRFLLGEYG-EVLGLDYKNIPENY 267
           YM  +K     + + QVELNTIS S   +S  V  LH++L+G       G D  N P N 
Sbjct: 122 YMFHNKIEGEEERIYQVELNTISSSLAVVSNRVFNLHKYLIGRNDLNDNGYDLLNHPTNQ 181

Query: 268 AANQYAEVLAKAWTEYN-DPSAVILFVIQAEERNMYDQLWLSVNLRKRHNIKTLRKTLAE 327
           +  + ++ +A A   YN + S+V+L +IQ  ERN+YDQ  L   L   H+IK +R+T+ E
Sbjct: 182 SDKEISDSIALAHKLYNKEKSSVVLMIIQEGERNIYDQKGLEFQLWSNHSIKLIRRTMKE 241

Query: 328 IDREGVL--RSDGTLVLDGQAIAVVYFRAGYTPRDYPSE--SEWSARLLMEQSSAVKCPS 387
           I++   L   +   L++DG  I+V Y+RAGYTP DY S    EW ARLL+E+S A+KCP+
Sbjct: 242 INQCAKLDEENGSVLIVDGMEISVAYYRAGYTPNDYTSSGGDEWKARLLIERSLAIKCPT 301

Query: 388 ISYHLVGTKKIQQELARPNVLERFLDSE-EDIAKIRKCFAGLWSLDDKDI----VKKAIE 447
           I++HLVG KKIQQ LA+P VLE+F++++ E + ++++ F GL+SL  +DI    VK+AIE
Sbjct: 302 IAHHLVGVKKIQQVLAQPGVLEKFINNDKESLQRVKRSFTGLYSLSKEDIDMSVVKEAIE 361

Query: 448 TPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDG 507
           +P+ +VMKPQREGGGNNIY D V  AL  +    SEE  +YILM +I    F T  VRD 
Sbjct: 362 SPQNYVMKPQREGGGNNIYNDQVAIALKSM---SSEELSSYILMDKIMSKSFKTHVVRDR 421

Query: 508 ICHKDHAVSELGIFGAYLRN-KDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDS 548
              +   + ELGI+  ++ N  D +++N+Q G L+RTK ++S+E GVAAGF +LDS
Sbjct: 422 QLLEIEGLYELGIYSVFISNGDDDIVLNKQAGILLRTKTANSDEVGVAAGFGLLDS 472

BLAST of Pay0005923.1 vs. ExPASy TrEMBL
Match: A0A1S3BQG6 (Glutathione synthetase OS=Cucumis melo OX=3656 GN=LOC103492200 PE=3 SV=1)

HSP 1 Score: 1087.8 bits (2812), Expect = 0.0e+00
Identity = 544/551 (98.73%), Postives = 549/551 (99.64%), Query Frame = 0

Query: 1   MGSSHSSCSLTPTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFKLQPFLFSSSSSSQVL 60
           MGSSHSSCSLTPTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFK QPFLFSSSSSSQVL
Sbjct: 1   MGSSHSSCSLTPTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFKSQPFLFSSSSSSQVL 60

Query: 61  PLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSGEV 120
           PLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSGEV
Sbjct: 61  PLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSGEV 120

Query: 121 PGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAFT 180
           PGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAFT
Sbjct: 121 PGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAFT 180

Query: 181 SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELH 240
           SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELH
Sbjct: 181 SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELH 240

Query: 241 RFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVIQAEERNMYDQL 300
           RFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFV+QAEERNMYDQL
Sbjct: 241 RFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVVQAEERNMYDQL 300

Query: 301 WLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVLDGQAIAVVYFRAGYTPRDYPSESE 360
           WLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLV+DGQAIAVVYFRAGYTPRDYPSESE
Sbjct: 301 WLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVVDGQAIAVVYFRAGYTPRDYPSESE 360

Query: 361 WSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGLWS 420
           WSARLLME SSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGLWS
Sbjct: 361 WSARLLMELSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGLWS 420

Query: 421 LDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQRIF 480
           LDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQ+EGSEEDGAYILMQRIF
Sbjct: 421 LDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQKEGSEEDGAYILMQRIF 480

Query: 481 PSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGGVAA 540
           PSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVN+Q+GYLMRTKVSSSNEGGVAA
Sbjct: 481 PSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNEQSGYLMRTKVSSSNEGGVAA 540

Query: 541 GFAVLDSIYLT 552
           GFAVLDSIYLT
Sbjct: 541 GFAVLDSIYLT 551

BLAST of Pay0005923.1 vs. ExPASy TrEMBL
Match: A0A0A0LW32 (Glutathione synthetase OS=Cucumis sativus OX=3659 GN=Csa_1G571280 PE=3 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 8.5e-291
Identity = 509/553 (92.04%), Postives = 528/553 (95.48%), Query Frame = 0

Query: 1   MGSSHS--SCSLTPTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFKLQPFLFSSSSSSQ 60
           MGSSHS  S SLTPTHSTKHFYFS     PSKLYPNQNQIRN INFK  PFL  SSSSSQ
Sbjct: 1   MGSSHSSRSLSLTPTHSTKHFYFS-----PSKLYPNQNQIRNLINFKSHPFL-CSSSSSQ 60

Query: 61  VLPLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSG 120
           VLPLKC KA EF+TQAKSL+TMADVHGID+ELLQEMVYDALVWSSLHGLVVGD+SVKRSG
Sbjct: 61  VLPLKCSKAGEFDTQAKSLRTMADVHGIDRELLQEMVYDALVWSSLHGLVVGDKSVKRSG 120

Query: 121 EVPGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDA 180
           EVPGVGLSHSPIALLPGLFP THWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDA
Sbjct: 121 EVPGVGLSHSPIALLPGLFPGTHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDA 180

Query: 181 FTSRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSE 240
           FTSRLLDIHSKMLQI KKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSE
Sbjct: 181 FTSRLLDIHSKMLQIGKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSE 240

Query: 241 LHRFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVIQAEERNMYD 300
           LHR LLGEYG+VLGLDY+N+PENYAAN +A+VLAKAW EYNDPSAVILFV+QAEERNMYD
Sbjct: 241 LHRALLGEYGKVLGLDYRNVPENYAANHFADVLAKAWIEYNDPSAVILFVVQAEERNMYD 300

Query: 301 QLWLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVLDGQAIAVVYFRAGYTPRDYPSE 360
           Q WLSVNLRKRHN+KTLRKTLAEIDREGVLRSDG L++DGQAIAVVYFRAGYTPRDYPSE
Sbjct: 301 QHWLSVNLRKRHNVKTLRKTLAEIDREGVLRSDGKLIVDGQAIAVVYFRAGYTPRDYPSE 360

Query: 361 SEWSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGL 420
           SEW ARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSE+DIAKIRKCFAGL
Sbjct: 361 SEWRARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEDDIAKIRKCFAGL 420

Query: 421 WSLDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQR 480
           WSLDD+DIV KAIETPELFVMKPQREGGGNNIYGDDVREALL+LQ+EGSEEDGAYILMQR
Sbjct: 421 WSLDDQDIVNKAIETPELFVMKPQREGGGNNIYGDDVREALLRLQKEGSEEDGAYILMQR 480

Query: 481 IFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGGV 540
           IFPSV PTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVN+Q+GYLMRTKVSSSNEGGV
Sbjct: 481 IFPSVSPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNEQSGYLMRTKVSSSNEGGV 540

Query: 541 AAGFAVLDSIYLT 552
           AAGFAVLDSIYLT
Sbjct: 541 AAGFAVLDSIYLT 547

BLAST of Pay0005923.1 vs. ExPASy TrEMBL
Match: A0A6J1E426 (Glutathione synthetase OS=Momordica charantia OX=3673 GN=LOC111025887 PE=3 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 5.6e-266
Identity = 464/551 (84.21%), Postives = 498/551 (90.38%), Query Frame = 0

Query: 1   MGSSHSSCSLTPTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFKLQPFLFSSSSSSQVL 60
           MGS+HSSCSL PT  T  F F P PI  S   PNQNQIRNSINF   PFL  SSSSS+VL
Sbjct: 1   MGSAHSSCSLIPTSPTNQFCFPPPPINSSNFRPNQNQIRNSINFTSHPFL--SSSSSKVL 60

Query: 61  PLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSGEV 120
           PL+C K  EFETQ K LKTMAD+HGIDQELLQ+MVYDALVWSSL+GLVV DRSVKRSG V
Sbjct: 61  PLRCSKVGEFETQVKFLKTMADIHGIDQELLQKMVYDALVWSSLNGLVVADRSVKRSGRV 120

Query: 121 PGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAFT 180
           PGVGL HSPIALLPGLFPET+W QACEL+SIFNELVDRVS+DGKFLQESLSRTKK D FT
Sbjct: 121 PGVGLVHSPIALLPGLFPETYWTQACELASIFNELVDRVSLDGKFLQESLSRTKKVDDFT 180

Query: 181 SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELH 240
           SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLD+KTKLLLQVELNTIS SF GLSCLVSELH
Sbjct: 181 SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDEKTKLLLQVELNTISSSFAGLSCLVSELH 240

Query: 241 RFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVIQAEERNMYDQL 300
             +L +YGEVLGLD + IP+N+A NQYAEVLAKAWTEYN+ SAVIL V+QAEERNMYDQ 
Sbjct: 241 SDILSQYGEVLGLDSRKIPKNHAVNQYAEVLAKAWTEYNNASAVILIVVQAEERNMYDQH 300

Query: 301 WLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVLDGQAIAVVYFRAGYTPRDYPSESE 360
           WLS NLRKRHN+KTLRKTLAEIDREG L+SDGTL++DGQAIAVVYFRAGYTP DYPSESE
Sbjct: 301 WLSYNLRKRHNVKTLRKTLAEIDREGELQSDGTLLVDGQAIAVVYFRAGYTPSDYPSESE 360

Query: 361 WSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGLWS 420
           W +RLLMEQSSA+KCPSISYHL GTKKIQQELA+PNVLERF+DS+EDIAKIRKCFAGLWS
Sbjct: 361 WRSRLLMEQSSAIKCPSISYHLAGTKKIQQELAKPNVLERFVDSKEDIAKIRKCFAGLWS 420

Query: 421 LDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQRIF 480
           LDD D VKKAIETP+LFVMKPQREGGGNNIYGDDVR+ALL+LQ+EG+EED AYILMQRIF
Sbjct: 421 LDDTDTVKKAIETPDLFVMKPQREGGGNNIYGDDVRKALLRLQKEGNEEDAAYILMQRIF 480

Query: 481 PSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGGVAA 540
           PSVFPTIFVRDGI HKDHA+SELGIFGAYLRN+DKVIVN   GYLMRTK SSSNEGGVAA
Sbjct: 481 PSVFPTIFVRDGIYHKDHAISELGIFGAYLRNRDKVIVNDHCGYLMRTKTSSSNEGGVAA 540

Query: 541 GFAVLDSIYLT 552
           GFAVLDS+YLT
Sbjct: 541 GFAVLDSVYLT 549

BLAST of Pay0005923.1 vs. ExPASy TrEMBL
Match: A0A6J1KP26 (Glutathione synthetase OS=Cucurbita maxima OX=3661 GN=LOC111495981 PE=3 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 5.4e-261
Identity = 462/552 (83.70%), Postives = 499/552 (90.40%), Query Frame = 0

Query: 1   MGSSHSSCSLTPTHSTKHFYFSPSPIAPSKLYP-NQNQIRNSINFKLQPFLFSSSSSSQV 60
           MGS+HSSCSL  T STKHF FS S I  SKL+P +QNQIR SI F   PF     SSSQ 
Sbjct: 7   MGSAHSSCSLVTTSSTKHFCFSQSSICVSKLHPTSQNQIR-SIKFTSHPF-HCCCSSSQH 66

Query: 61  LPLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSGE 120
           LPL+C +  EFE+Q KSLKTMAD+HGIDQELL++MVYDALVWSSLHGLVVGD+SVKRSG+
Sbjct: 67  LPLRCNQVGEFESQVKSLKTMADIHGIDQELLKKMVYDALVWSSLHGLVVGDKSVKRSGK 126

Query: 121 VPGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAF 180
           VPGVGL H+PIALLPGLFPETHW QACEL SIFNELVDRVS+DGKFLQESLSRTKK DAF
Sbjct: 127 VPGVGLVHAPIALLPGLFPETHWKQACELVSIFNELVDRVSLDGKFLQESLSRTKKVDAF 186

Query: 181 TSRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSEL 240
           TSRLLDIHSKMLQIDKKEEIRLGLHRSDYMLD+KTKLLLQVELNTISCSF   S LVS+L
Sbjct: 187 TSRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDEKTKLLLQVELNTISCSFAEFSHLVSQL 246

Query: 241 HRFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVIQAEERNMYDQ 300
           H  LLG+YGEVLGLD + IPEN+A NQYAEVL KAWTEYN+PSAVI+ V+QAEERNMYDQ
Sbjct: 247 HSDLLGQYGEVLGLDSRMIPENHAGNQYAEVLVKAWTEYNNPSAVIMIVVQAEERNMYDQ 306

Query: 301 LWLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVLDGQAIAVVYFRAGYTPRDYPSES 360
             LSVNLRKRH IKTLRKTLAEIDREG L+SDGTL++DGQA+AVVY+RAGYTP DYPSE 
Sbjct: 307 HCLSVNLRKRHYIKTLRKTLAEIDREGELQSDGTLLVDGQAVAVVYYRAGYTPTDYPSEL 366

Query: 361 EWSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGLW 420
           EW ARLLMEQSSAVKCPSISYHLVGTKKIQQELA+PNVLERFLD++EDIAKIRKCFAGLW
Sbjct: 367 EWKARLLMEQSSAVKCPSISYHLVGTKKIQQELAQPNVLERFLDNKEDIAKIRKCFAGLW 426

Query: 421 SLDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQRI 480
           SLDDKDIVKKAIE P+LFVMKPQREGGGNNIYGDDVR+ L++LQ+EG+ ED AYILMQRI
Sbjct: 427 SLDDKDIVKKAIEAPDLFVMKPQREGGGNNIYGDDVRKTLVRLQKEGNVEDAAYILMQRI 486

Query: 481 FPSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGGVA 540
           FPSV+PTIFVRDGICHKDHA+SELGIFGAYLRNKDKVIVN Q GYLM TKVSSSNEGGVA
Sbjct: 487 FPSVYPTIFVRDGICHKDHAISELGIFGAYLRNKDKVIVNDQCGYLMTTKVSSSNEGGVA 546

Query: 541 AGFAVLDSIYLT 552
           AGFAVLDSIYLT
Sbjct: 547 AGFAVLDSIYLT 556

BLAST of Pay0005923.1 vs. ExPASy TrEMBL
Match: K9JF36 (Glutathione synthetase OS=Cucumis sativus OX=3659 GN=GSH2 PE=2 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 5.4e-261
Identity = 448/472 (94.92%), Postives = 465/472 (98.52%), Query Frame = 0

Query: 80  MADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSGEVPGVGLSHSPIALLPGLFPE 139
           MADVHGID+ELLQEMVYDALVWSSLHGLVVGD+SVKRSGEVPGVGLSHSPIALLPGLFP 
Sbjct: 1   MADVHGIDRELLQEMVYDALVWSSLHGLVVGDKSVKRSGEVPGVGLSHSPIALLPGLFPG 60

Query: 140 THWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAFTSRLLDIHSKMLQIDKKEEI 199
           THWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAFTSRLLDIHSKMLQIDKKEEI
Sbjct: 61  THWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAFTSRLLDIHSKMLQIDKKEEI 120

Query: 200 RLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELHRFLLGEYGEVLGLDYKNIP 259
           RLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELHR LLGEYG+VLGLDY+N+P
Sbjct: 121 RLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELHRALLGEYGKVLGLDYRNVP 180

Query: 260 ENYAANQYAEVLAKAWTEYNDPSAVILFVIQAEERNMYDQLWLSVNLRKRHNIKTLRKTL 319
           ENYAANQ+A+VLAKAW EYNDPSAVILFV+QAEERNMYDQ WLSVNLRKRHN+KTLRKTL
Sbjct: 181 ENYAANQFADVLAKAWIEYNDPSAVILFVVQAEERNMYDQHWLSVNLRKRHNVKTLRKTL 240

Query: 320 AEIDREGVLRSDGTLVLDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSIS 379
           AEIDREGVLRSDG L++DGQAIAVVYFRAGYTPRDYPSESEW ARLLMEQSSAVKCPSIS
Sbjct: 241 AEIDREGVLRSDGKLIVDGQAIAVVYFRAGYTPRDYPSESEWRARLLMEQSSAVKCPSIS 300

Query: 380 YHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVM 439
           YHLVGTKKIQQELARPNVLERFLDSE+DIAKIRKCFAGLWSLDD+DIVKKAIETPELFVM
Sbjct: 301 YHLVGTKKIQQELARPNVLERFLDSEDDIAKIRKCFAGLWSLDDQDIVKKAIETPELFVM 360

Query: 440 KPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHA 499
           KPQREGGGNNIYGDDVREALL+LQ+EGSEEDGAYILMQRIFPSV PTIFVRDGICHKDHA
Sbjct: 361 KPQREGGGNNIYGDDVREALLRLQKEGSEEDGAYILMQRIFPSVSPTIFVRDGICHKDHA 420

Query: 500 VSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 552
           VSELGIFGAYLRNKDKVIVN+Q+GYLMRTKVSSSNEGGVAAGFAVLDSIYLT
Sbjct: 421 VSELGIFGAYLRNKDKVIVNEQSGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 472

BLAST of Pay0005923.1 vs. NCBI nr
Match: XP_008450698.1 (PREDICTED: glutathione synthetase, chloroplastic [Cucumis melo])

HSP 1 Score: 1087.8 bits (2812), Expect = 0.0e+00
Identity = 544/551 (98.73%), Postives = 549/551 (99.64%), Query Frame = 0

Query: 1   MGSSHSSCSLTPTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFKLQPFLFSSSSSSQVL 60
           MGSSHSSCSLTPTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFK QPFLFSSSSSSQVL
Sbjct: 1   MGSSHSSCSLTPTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFKSQPFLFSSSSSSQVL 60

Query: 61  PLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSGEV 120
           PLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSGEV
Sbjct: 61  PLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSGEV 120

Query: 121 PGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAFT 180
           PGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAFT
Sbjct: 121 PGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAFT 180

Query: 181 SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELH 240
           SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELH
Sbjct: 181 SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELH 240

Query: 241 RFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVIQAEERNMYDQL 300
           RFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFV+QAEERNMYDQL
Sbjct: 241 RFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVVQAEERNMYDQL 300

Query: 301 WLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVLDGQAIAVVYFRAGYTPRDYPSESE 360
           WLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLV+DGQAIAVVYFRAGYTPRDYPSESE
Sbjct: 301 WLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVVDGQAIAVVYFRAGYTPRDYPSESE 360

Query: 361 WSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGLWS 420
           WSARLLME SSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGLWS
Sbjct: 361 WSARLLMELSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGLWS 420

Query: 421 LDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQRIF 480
           LDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQ+EGSEEDGAYILMQRIF
Sbjct: 421 LDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQKEGSEEDGAYILMQRIF 480

Query: 481 PSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGGVAA 540
           PSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVN+Q+GYLMRTKVSSSNEGGVAA
Sbjct: 481 PSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNEQSGYLMRTKVSSSNEGGVAA 540

Query: 541 GFAVLDSIYLT 552
           GFAVLDSIYLT
Sbjct: 541 GFAVLDSIYLT 551

BLAST of Pay0005923.1 vs. NCBI nr
Match: XP_004135633.1 (glutathione synthetase, chloroplastic [Cucumis sativus])

HSP 1 Score: 1008.8 bits (2607), Expect = 1.8e-290
Identity = 509/553 (92.04%), Postives = 528/553 (95.48%), Query Frame = 0

Query: 1   MGSSHS--SCSLTPTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFKLQPFLFSSSSSSQ 60
           MGSSHS  S SLTPTHSTKHFYFS     PSKLYPNQNQIRN INFK  PFL  SSSSSQ
Sbjct: 1   MGSSHSSRSLSLTPTHSTKHFYFS-----PSKLYPNQNQIRNLINFKSHPFL-CSSSSSQ 60

Query: 61  VLPLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSG 120
           VLPLKC KA EF+TQAKSL+TMADVHGID+ELLQEMVYDALVWSSLHGLVVGD+SVKRSG
Sbjct: 61  VLPLKCSKAGEFDTQAKSLRTMADVHGIDRELLQEMVYDALVWSSLHGLVVGDKSVKRSG 120

Query: 121 EVPGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDA 180
           EVPGVGLSHSPIALLPGLFP THWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDA
Sbjct: 121 EVPGVGLSHSPIALLPGLFPGTHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDA 180

Query: 181 FTSRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSE 240
           FTSRLLDIHSKMLQI KKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSE
Sbjct: 181 FTSRLLDIHSKMLQIGKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSE 240

Query: 241 LHRFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVIQAEERNMYD 300
           LHR LLGEYG+VLGLDY+N+PENYAAN +A+VLAKAW EYNDPSAVILFV+QAEERNMYD
Sbjct: 241 LHRALLGEYGKVLGLDYRNVPENYAANHFADVLAKAWIEYNDPSAVILFVVQAEERNMYD 300

Query: 301 QLWLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVLDGQAIAVVYFRAGYTPRDYPSE 360
           Q WLSVNLRKRHN+KTLRKTLAEIDREGVLRSDG L++DGQAIAVVYFRAGYTPRDYPSE
Sbjct: 301 QHWLSVNLRKRHNVKTLRKTLAEIDREGVLRSDGKLIVDGQAIAVVYFRAGYTPRDYPSE 360

Query: 361 SEWSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGL 420
           SEW ARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSE+DIAKIRKCFAGL
Sbjct: 361 SEWRARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEDDIAKIRKCFAGL 420

Query: 421 WSLDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQR 480
           WSLDD+DIV KAIETPELFVMKPQREGGGNNIYGDDVREALL+LQ+EGSEEDGAYILMQR
Sbjct: 421 WSLDDQDIVNKAIETPELFVMKPQREGGGNNIYGDDVREALLRLQKEGSEEDGAYILMQR 480

Query: 481 IFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGGV 540
           IFPSV PTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVN+Q+GYLMRTKVSSSNEGGV
Sbjct: 481 IFPSVSPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNEQSGYLMRTKVSSSNEGGV 540

Query: 541 AAGFAVLDSIYLT 552
           AAGFAVLDSIYLT
Sbjct: 541 AAGFAVLDSIYLT 547

BLAST of Pay0005923.1 vs. NCBI nr
Match: XP_038877693.1 (glutathione synthetase, chloroplastic [Benincasa hispida])

HSP 1 Score: 992.3 bits (2564), Expect = 1.7e-285
Identity = 498/551 (90.38%), Postives = 520/551 (94.37%), Query Frame = 0

Query: 1   MGSSHSSCSLTPTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFKLQPFLFSSSSSSQVL 60
           MGS+HSSCSLTPT STKHF FS     PSKLYPNQN+IRN I F   PFL  S  SSQVL
Sbjct: 1   MGSAHSSCSLTPTSSTKHFCFS-----PSKLYPNQNRIRNFIKFTSHPFL-CSYYSSQVL 60

Query: 61  PLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSGEV 120
           PLKC KA EFETQ KSLKTMAD+HGIDQELLQ+MVYDALVWSSLHGLVVGD+SVKRSGEV
Sbjct: 61  PLKCSKAVEFETQVKSLKTMADIHGIDQELLQKMVYDALVWSSLHGLVVGDKSVKRSGEV 120

Query: 121 PGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAFT 180
           PGVGLSHSPIALLPGLFPETH  QACELSSIFNELVDRVSMDGKFLQESLSRTKKGD FT
Sbjct: 121 PGVGLSHSPIALLPGLFPETHRKQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDVFT 180

Query: 181 SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELH 240
           SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELH
Sbjct: 181 SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELH 240

Query: 241 RFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVIQAEERNMYDQL 300
           R LL +YGEVLGLDY+ IPEN+AANQYAEVLAKAWTEYNDPSAVI+FV+Q EERNMYDQ 
Sbjct: 241 RDLLSQYGEVLGLDYRKIPENHAANQYAEVLAKAWTEYNDPSAVIMFVVQVEERNMYDQH 300

Query: 301 WLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVLDGQAIAVVYFRAGYTPRDYPSESE 360
           WLSVNLRKRHN+KTLRKTLAE+DREGVLR+DGTL++DGQAIAVVYFRAGYTPRDYPSESE
Sbjct: 301 WLSVNLRKRHNVKTLRKTLAEVDREGVLRTDGTLLVDGQAIAVVYFRAGYTPRDYPSESE 360

Query: 361 WSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGLWS 420
           W ARLLMEQSSAVKCPSISYHLVGTKKIQQELA+PNVLERFLDS+EDIAKIRKCFAGLWS
Sbjct: 361 WRARLLMEQSSAVKCPSISYHLVGTKKIQQELAKPNVLERFLDSKEDIAKIRKCFAGLWS 420

Query: 421 LDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQRIF 480
           LDDKDIVKKAIETP+LFVMKPQREGGGNNIYGDDVREALL+LQ+EGSEED AYILMQRIF
Sbjct: 421 LDDKDIVKKAIETPDLFVMKPQREGGGNNIYGDDVREALLRLQKEGSEEDAAYILMQRIF 480

Query: 481 PSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGGVAA 540
           PSVFPTIFVRDGICHKDHA+SELGIFGAYLRNKDKVIVN  +GYLMRTKVSSSNEGGVAA
Sbjct: 481 PSVFPTIFVRDGICHKDHAISELGIFGAYLRNKDKVIVNDHSGYLMRTKVSSSNEGGVAA 540

Query: 541 GFAVLDSIYLT 552
           GFAVLDSIYLT
Sbjct: 541 GFAVLDSIYLT 545

BLAST of Pay0005923.1 vs. NCBI nr
Match: XP_022159486.1 (glutathione synthetase, chloroplastic [Momordica charantia])

HSP 1 Score: 926.4 bits (2393), Expect = 1.2e-265
Identity = 464/551 (84.21%), Postives = 498/551 (90.38%), Query Frame = 0

Query: 1   MGSSHSSCSLTPTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFKLQPFLFSSSSSSQVL 60
           MGS+HSSCSL PT  T  F F P PI  S   PNQNQIRNSINF   PFL  SSSSS+VL
Sbjct: 1   MGSAHSSCSLIPTSPTNQFCFPPPPINSSNFRPNQNQIRNSINFTSHPFL--SSSSSKVL 60

Query: 61  PLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSGEV 120
           PL+C K  EFETQ K LKTMAD+HGIDQELLQ+MVYDALVWSSL+GLVV DRSVKRSG V
Sbjct: 61  PLRCSKVGEFETQVKFLKTMADIHGIDQELLQKMVYDALVWSSLNGLVVADRSVKRSGRV 120

Query: 121 PGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAFT 180
           PGVGL HSPIALLPGLFPET+W QACEL+SIFNELVDRVS+DGKFLQESLSRTKK D FT
Sbjct: 121 PGVGLVHSPIALLPGLFPETYWTQACELASIFNELVDRVSLDGKFLQESLSRTKKVDDFT 180

Query: 181 SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSELH 240
           SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLD+KTKLLLQVELNTIS SF GLSCLVSELH
Sbjct: 181 SRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDEKTKLLLQVELNTISSSFAGLSCLVSELH 240

Query: 241 RFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVIQAEERNMYDQL 300
             +L +YGEVLGLD + IP+N+A NQYAEVLAKAWTEYN+ SAVIL V+QAEERNMYDQ 
Sbjct: 241 SDILSQYGEVLGLDSRKIPKNHAVNQYAEVLAKAWTEYNNASAVILIVVQAEERNMYDQH 300

Query: 301 WLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVLDGQAIAVVYFRAGYTPRDYPSESE 360
           WLS NLRKRHN+KTLRKTLAEIDREG L+SDGTL++DGQAIAVVYFRAGYTP DYPSESE
Sbjct: 301 WLSYNLRKRHNVKTLRKTLAEIDREGELQSDGTLLVDGQAIAVVYFRAGYTPSDYPSESE 360

Query: 361 WSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGLWS 420
           W +RLLMEQSSA+KCPSISYHL GTKKIQQELA+PNVLERF+DS+EDIAKIRKCFAGLWS
Sbjct: 361 WRSRLLMEQSSAIKCPSISYHLAGTKKIQQELAKPNVLERFVDSKEDIAKIRKCFAGLWS 420

Query: 421 LDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQRIF 480
           LDD D VKKAIETP+LFVMKPQREGGGNNIYGDDVR+ALL+LQ+EG+EED AYILMQRIF
Sbjct: 421 LDDTDTVKKAIETPDLFVMKPQREGGGNNIYGDDVRKALLRLQKEGNEEDAAYILMQRIF 480

Query: 481 PSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGGVAA 540
           PSVFPTIFVRDGI HKDHA+SELGIFGAYLRN+DKVIVN   GYLMRTK SSSNEGGVAA
Sbjct: 481 PSVFPTIFVRDGIYHKDHAISELGIFGAYLRNRDKVIVNDHCGYLMRTKTSSSNEGGVAA 540

Query: 541 GFAVLDSIYLT 552
           GFAVLDS+YLT
Sbjct: 541 GFAVLDSVYLT 549

BLAST of Pay0005923.1 vs. NCBI nr
Match: XP_023001954.1 (glutathione synthetase, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 909.8 bits (2350), Expect = 1.1e-260
Identity = 462/552 (83.70%), Postives = 499/552 (90.40%), Query Frame = 0

Query: 1   MGSSHSSCSLTPTHSTKHFYFSPSPIAPSKLYP-NQNQIRNSINFKLQPFLFSSSSSSQV 60
           MGS+HSSCSL  T STKHF FS S I  SKL+P +QNQIR SI F   PF     SSSQ 
Sbjct: 7   MGSAHSSCSLVTTSSTKHFCFSQSSICVSKLHPTSQNQIR-SIKFTSHPF-HCCCSSSQH 66

Query: 61  LPLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRSGE 120
           LPL+C +  EFE+Q KSLKTMAD+HGIDQELL++MVYDALVWSSLHGLVVGD+SVKRSG+
Sbjct: 67  LPLRCNQVGEFESQVKSLKTMADIHGIDQELLKKMVYDALVWSSLHGLVVGDKSVKRSGK 126

Query: 121 VPGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGDAF 180
           VPGVGL H+PIALLPGLFPETHW QACEL SIFNELVDRVS+DGKFLQESLSRTKK DAF
Sbjct: 127 VPGVGLVHAPIALLPGLFPETHWKQACELVSIFNELVDRVSLDGKFLQESLSRTKKVDAF 186

Query: 181 TSRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVSEL 240
           TSRLLDIHSKMLQIDKKEEIRLGLHRSDYMLD+KTKLLLQVELNTISCSF   S LVS+L
Sbjct: 187 TSRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDEKTKLLLQVELNTISCSFAEFSHLVSQL 246

Query: 241 HRFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVIQAEERNMYDQ 300
           H  LLG+YGEVLGLD + IPEN+A NQYAEVL KAWTEYN+PSAVI+ V+QAEERNMYDQ
Sbjct: 247 HSDLLGQYGEVLGLDSRMIPENHAGNQYAEVLVKAWTEYNNPSAVIMIVVQAEERNMYDQ 306

Query: 301 LWLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVLDGQAIAVVYFRAGYTPRDYPSES 360
             LSVNLRKRH IKTLRKTLAEIDREG L+SDGTL++DGQA+AVVY+RAGYTP DYPSE 
Sbjct: 307 HCLSVNLRKRHYIKTLRKTLAEIDREGELQSDGTLLVDGQAVAVVYYRAGYTPTDYPSEL 366

Query: 361 EWSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGLW 420
           EW ARLLMEQSSAVKCPSISYHLVGTKKIQQELA+PNVLERFLD++EDIAKIRKCFAGLW
Sbjct: 367 EWKARLLMEQSSAVKCPSISYHLVGTKKIQQELAQPNVLERFLDNKEDIAKIRKCFAGLW 426

Query: 421 SLDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQRI 480
           SLDDKDIVKKAIE P+LFVMKPQREGGGNNIYGDDVR+ L++LQ+EG+ ED AYILMQRI
Sbjct: 427 SLDDKDIVKKAIEAPDLFVMKPQREGGGNNIYGDDVRKTLVRLQKEGNVEDAAYILMQRI 486

Query: 481 FPSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGGVA 540
           FPSV+PTIFVRDGICHKDHA+SELGIFGAYLRNKDKVIVN Q GYLM TKVSSSNEGGVA
Sbjct: 487 FPSVYPTIFVRDGICHKDHAISELGIFGAYLRNKDKVIVNDQCGYLMTTKVSSSNEGGVA 546

Query: 541 AGFAVLDSIYLT 552
           AGFAVLDSIYLT
Sbjct: 547 AGFAVLDSIYLT 556

BLAST of Pay0005923.1 vs. TAIR 10
Match: AT5G27380.1 (glutathione synthetase 2 )

HSP 1 Score: 697.2 bits (1798), Expect = 1.1e-200
Identity = 350/553 (63.29%), Postives = 442/553 (79.93%), Query Frame = 0

Query: 1   MGSSHSSCSLTPTHSTKHFYFSPSPIAPSKLYPNQNQIRNSINFKLQP--FLFSSSSS-S 60
           MGS  SS S + + +     FS S  +PS          +S + KL P  FLF +  +  
Sbjct: 1   MGSGCSSLSYSSSSTCNATVFSISS-SPS----------SSSSLKLNPSSFLFQNPKTLR 60

Query: 61  QVLPLKCRKAAEFETQAKSLKTMADVHGIDQELLQEMVYDALVWSSLHGLVVGDRSVKRS 120
              PL+C ++ + E+Q    K + D+  +D E +Q++VYDALVWSSLHGLVVGD+S ++S
Sbjct: 61  NQSPLRCGRSFKMESQ----KPIFDLEKLDDEFVQKLVYDALVWSSLHGLVVGDKSYQKS 120

Query: 121 GEVPGVGLSHSPIALLPGLFPETHWNQACELSSIFNELVDRVSMDGKFLQESLSRTKKGD 180
           G VPGVGL H+PIALLP  FPE +W QAC ++ +FNEL+DRVS+DGKFLQ+SLSRTKK D
Sbjct: 121 GNVPGVGLMHAPIALLPTAFPEAYWKQACNVTPLFNELIDRVSLDGKFLQDSLSRTKKVD 180

Query: 181 AFTSRLLDIHSKMLQIDKKEEIRLGLHRSDYMLDDKTKLLLQVELNTISCSFPGLSCLVS 240
            FTSRLLDIHSKML+ +KKE+IRLGLHR DYMLD++T  LLQ+E+NTISCSFPGLS LVS
Sbjct: 181 VFTSRLLDIHSKMLERNKKEDIRLGLHRFDYMLDEETNSLLQIEMNTISCSFPGLSRLVS 240

Query: 241 ELHRFLLGEYGEVLGLDYKNIPENYAANQYAEVLAKAWTEYNDPSAVILFVIQAEERNMY 300
           +LH+ LL  YG+ +G+D + +P N +  Q+A+ LAKAW EY++P AV++ ++Q EERNMY
Sbjct: 241 QLHQSLLRSYGDQIGIDSERVPINTSTIQFADALAKAWLEYSNPRAVVMVIVQPEERNMY 300

Query: 301 DQLWLSVNLRKRHNIKTLRKTLAEIDREGVLRSDGTLVLDGQAIAVVYFRAGYTPRDYPS 360
           DQ  LS  LR++HNI  +RKTLAE+++EG ++ D TL++ GQA+AVVYFR+GYTP D+PS
Sbjct: 301 DQHLLSSILREKHNIVVIRKTLAEVEKEGSVQEDETLIVGGQAVAVVYFRSGYTPNDHPS 360

Query: 361 ESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAG 420
           ESEW+ARLL+E+SSAVKCPSI+YHL G+KKIQQELA+P VLERFLD++EDIAK+RKCFAG
Sbjct: 361 ESEWNARLLIEESSAVKCPSIAYHLTGSKKIQQELAKPGVLERFLDNKEDIAKLRKCFAG 420

Query: 421 LWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQ 480
           LWSLDD +IVK+AIE P LFVMKPQREGGGNNIYGDDVRE LL+LQ+EG E + AYILMQ
Sbjct: 421 LWSLDDSEIVKQAIEKPGLFVMKPQREGGGNNIYGDDVRENLLRLQKEGEEGNAAYILMQ 480

Query: 481 RIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGG 540
           RIFP V     VR+G+ HK  A+SELG++GAYLR+KD+VIVN+Q+GYLMRTK++SS+EGG
Sbjct: 481 RIFPKVSNMFLVREGVYHKHQAISELGVYGAYLRSKDEVIVNEQSGYLMRTKIASSDEGG 538

Query: 541 VAAGFAVLDSIYL 551
           VAAGF VLDSIYL
Sbjct: 541 VAAGFGVLDSIYL 538