PI0029177.1 (mRNA) Melon (PI 482460) v1

Overview


Name	PI0029177.1
Type	mRNA
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	MBOAT (membrane bound O-acyl transferase) family protein
Location	chr01: 12367327 .. 12368390 (+)
Sequence length	1029
RNA-Seq Expression	PI0029177.1
Synteny	PI0029177.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGTCATGTATGGAGAACTCAAAAGCTTCATGAAAGTCTCTCTCACAATCATGATCTCTCTATTATACTCATATTTTATATCTTCAAAGCTTCCCAAAGGCAAATTTAGGCTCATCTCTCTCTTCCCTATTTTCTTTCTCTTCGCCCTTCTTCCCCTCTCTCTGTCCTCTATCTTCCTCACCGGTACCGTCGCCTTCTTCATCACTTGGCTCACAACCTTCAAACTTCTTCTATTTTCATTCAATTTAGGTCCTCTTGTTTCCGACCCACCATTGACATTCCCTCTCTTCGCTTCAATTGCTTGTCTTCCAATCAAAATAAAGCCAAAAAAAATAGACCCAGATTATAAATATCTCGAAAAATATGCAAATCCCAAGTTGGGCCTAAATTTGCCTGCCAAAATCTTGATTTTTGCCATCTTGATCGCCGTCGATGACCACATTGACCAGCTTTGGCCAAATGTGAAGATTTGTGTCTACTGTGTGACACTATATCTCTTCGTTGATTTTCTTCTTGGAGTGTCGAGTGCTATTGTTGGATCGGCATTCGATGGTGTTGTGCTTGAACCATCGTCAAATGAGCCTTACTTGGCAACATCTCTCCAAGATTTTTGGGGCAGGAGGTGGAATTTGTTGGTGTCGAATACTTTGCGTTATGGCATTTACAGGCCGGTTCGAGCAATAGTGGATGGTGTAGTGGGGAAGAGGTGGGTGGCCGGAACGGGGTTATGGCGGTGTTTGTCGCTTCGGGAGTGATGCATGAGCAATTGGTGTATTACATTACTAGAGAAGTGCCAACTTGGGATGTCACATGGTTCTTTATTCTTCAGGGGGTGTGTGTGGTGGTGGAATTTGAGTTAAAGACGGTTGTAGGACGACGATGGCAGCCACCTACAGTGGTGGCATGGCTGTTAACGTTGTCGTTTTTGGTTGTCACTGCTTCATGGTTCTTTTTTCCGCCACTAATGAGAGCTGGTACTTTTGCTGGACTTCTTAAGGAATTGAAGATGGTGGAAGAGTTTGTGTGGAAACTCACTTTAAGTTTATTCCGAAAATACTAG

mRNA sequence

ATGGTTGTCATGTATGGAGAACTCAAAAGCTTCATGAAAGTCTCTCTCACAATCATGATCTCTCTATTATACTCATATTTTATATCTTCAAAGCTTCCCAAAGGCAAATTTAGGCTCATCTCTCTCTTCCCTATTTTCTTTCTCTTCGCCCTTCTTCCCCTCTCTCTGTCCTCTATCTTCCTCACCGGTACCGTCGCCTTCTTCATCACTTGGCTCACAACCTTCAAACTTCTTCTATTTTCATTCAATTTAGGTCCTCTTGTTTCCGACCCACCATTGACATTCCCTCTCTTCGCTTCAATTGCTTGTCTTCCAATCAAAATAAAGCCAAAAAAAATAGACCCAGATTATAAATATCTCGAAAAATATGCAAATCCCAAGTTGGGCCTAAATTTGCCTGCCAAAATCTTGATTTTTGCCATCTTGATCGCCGTCGATGACCACATTGACCAGCTTTGGCCAAATGTGAAGATTTGTGTCTACTGTGTGACACTATATCTCTTCGTTGATTTTCTTCTTGGAGTGTCGAGTGCTATTGTTGGATCGGCATTCGATGGTGTTGTGCTTGAACCATCGTCAAATGAGCCTTACTTGGCAACATCTCTCCAAGATTTTTGGGGCAGGAGGTGGAATTTGTTGGTGTCGAATACTTTGCGTTATGGCATTTACAGGCCGGTGGGTGGCCGGAACGGGGTTATGGCGGTGTTTGTCGCTTCGGGAGTGATGCATGAGCAATTGGTGTATTACATTACTAGAGAAGTGCCAACTTGGGATGTCACATGGTTCTTTATTCTTCAGGGGGTGTGTGTGGTGGTGGAATTTGAGTTAAAGACGGTTGTAGGACGACGATGGCAGCCACCTACAGTGGTGGCATGGCTGTTAACGTTGTCGTTTTTGGTTGTCACTGCTTCATGGTTCTTTTTTCCGCCACTAATGAGAGCTGGTACTTTTGCTGGACTTCTTAAGGAATTGAAGATGGTGGAAGAGTTTGTGTGGAAACTCACTTTAAGTTTATTCCGAAAATACTAG

Coding sequence (CDS)

ATGGTTGTCATGTATGGAGAACTCAAAAGCTTCATGAAAGTCTCTCTCACAATCATGATCTCTCTATTATACTCATATTTTATATCTTCAAAGCTTCCCAAAGGCAAATTTAGGCTCATCTCTCTCTTCCCTATTTTCTTTCTCTTCGCCCTTCTTCCCCTCTCTCTGTCCTCTATCTTCCTCACCGGTACCGTCGCCTTCTTCATCACTTGGCTCACAACCTTCAAACTTCTTCTATTTTCATTCAATTTAGGTCCTCTTGTTTCCGACCCACCATTGACATTCCCTCTCTTCGCTTCAATTGCTTGTCTTCCAATCAAAATAAAGCCAAAAAAAATAGACCCAGATTATAAATATCTCGAAAAATATGCAAATCCCAAGTTGGGCCTAAATTTGCCTGCCAAAATCTTGATTTTTGCCATCTTGATCGCCGTCGATGACCACATTGACCAGCTTTGGCCAAATGTGAAGATTTGTGTCTACTGTGTGACACTATATCTCTTCGTTGATTTTCTTCTTGGAGTGTCGAGTGCTATTGTTGGATCGGCATTCGATGGTGTTGTGCTTGAACCATCGTCAAATGAGCCTTACTTGGCAACATCTCTCCAAGATTTTTGGGGCAGGAGGTGGAATTTGTTGGTGTCGAATACTTTGCGTTATGGCATTTACAGGCCGGTGGGTGGCCGGAACGGGGTTATGGCGGTGTTTGTCGCTTCGGGAGTGATGCATGAGCAATTGGTGTATTACATTACTAGAGAAGTGCCAACTTGGGATGTCACATGGTTCTTTATTCTTCAGGGGGTGTGTGTGGTGGTGGAATTTGAGTTAAAGACGGTTGTAGGACGACGATGGCAGCCACCTACAGTGGTGGCATGGCTGTTAACGTTGTCGTTTTTGGTTGTCACTGCTTCATGGTTCTTTTTTCCGCCACTAATGAGAGCTGGTACTTTTGCTGGACTTCTTAAGGAATTGAAGATGGTGGAAGAGTTTGTGTGGAAACTCACTTTAAGTTTATTCCGAAAATACTAG

Protein sequence

MVVMYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTGTVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYLEKYANPKLGLNLPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIVGSAFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPVGGRNGVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELKTVVGRRWQPPTVVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFVWKLTLSLFRKY

Homology

[Close]

BLAST of PI0029177.1 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.0e-57
Identity = 144/343 (41.98%), Postives = 207/343 (60.35%), Query Frame = 0


Query: 4   MYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTG 63
           M  ELK+F+K+ ++ +IS+ Y Y++S+ +  G FRL+S+ P+  LF + PL  S +  +G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 64  TVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYLEKY 123
            +AFF++WL  FKL+LFSF+ GPL S  P T   F  I C P  IKP++ +P+ +     
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL-SPLPRTLSRFICITCFP--IKPQQ-NPNIQ----- 120

Query: 124 ANPKLGLNLPA-KILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIVGS 183
            N K+ + L A K++IF +L+ + ++   L P + +    + ++L ++ +  +  A+V  
Sbjct: 121 -NYKIPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFI 180

Query: 184 AFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPVGGRN------------ 243
              G  LEP SNEPYLATSLQDFWGRRWNL+V   LR  +Y P                 
Sbjct: 181 TL-GCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFL 240

Query: 244 GVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELK--TVVGRRWQPPT 303
           GV A F+ SG +HE L +Y+TREVPT +VTWFF+L GVC V E  +K  T V R W+   
Sbjct: 241 GVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSP 300

Query: 304 VVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFV 332
            V+ LLT+ F+VVT+ WFFF PL+R+G    L  E  M  +FV
Sbjct: 301 TVSRLLTVGFVVVTSGWFFF-PLIRSGIIERLASEALMCIDFV 331

[Close]

BLAST of PI0029177.1 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.3e-57
Identity = 138/344 (40.12%), Postives = 197/344 (57.27%), Query Frame = 0


Query: 7   ELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTGTVA 66
           ELK+ +KV ++ +IS+ Y Y+I  ++  G  R +S+ P+  LF +LPL  SS+ L+   A
Sbjct: 4   ELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITA 63

Query: 67  FFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYLEKYANP 126
           FF+TWL  FKL+LFSF+ GPL+   P  FP F    C PIK++               NP
Sbjct: 64  FFLTWLANFKLILFSFDKGPLI-PIPTNFPRFFCFTCFPIKVQ--------------QNP 123

Query: 127 KLGLNLP-----AKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIVG 186
           K   +LP      K+ IFA+L+ +  +   L P + + +Y V LYL ++ +L     +V 
Sbjct: 124 KSQNHLPKLVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVF 183

Query: 187 SAFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRP---VGGRN-------- 246
            +  G  LEP SN+PYLATSLQDFWGRRWNL+V   LR  +Y P   V  R         
Sbjct: 184 ISL-GCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 243

Query: 247 -GVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFEL--KTVVGRRWQPP 306
            G++A F+ SG++HE L +Y+ RE+PT +VT FF+L GVC  VE  +  KT V +RW+  
Sbjct: 244 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 303

Query: 307 TVVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFV 332
             V+ +LT+ F+ VT  W F P L R+G       E  ++ EF+
Sbjct: 304 PGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFI 331

[Close]

BLAST of PI0029177.1 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.8e-55
Identity = 132/349 (37.82%), Postives = 193/349 (55.30%), Query Frame = 0


Query: 8   LKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTGTVAF 67
           + SF+K    ++ISL Y++FI+  +PKG  RLI  FP+F +F ++P  + S+ L G  AF
Sbjct: 1   MASFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAF 60

Query: 68  FITWLTTFKLLLFSFNLGPLVSD-PPLTFPLFASIACLPIKIKPKKIDPDYKYLEKYANP 127
           FI WL  FKLLLF+   GPL S+  PL+ P+F +++CLPIKI   ++ P       +   
Sbjct: 61  FIAWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKI---QLSPKPTKTHSHEGS 120

Query: 128 KLG-LNLPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIVGSAFD 187
             G L    K +   ++I   ++  +L   V + +Y + +Y  ++ +L  ++A V  A  
Sbjct: 121 TEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAV-RAMS 180

Query: 188 GVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRP------VGGRN-----GVMA 247
            + LEP  N+PYLATSLQDFWGRRWNL+V+  LR  +Y P      V G N         
Sbjct: 181 DLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLGPNYSQILAAFG 240

Query: 248 VFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELKTVVGRRWQPPTVVAWLL 307
            FV SG+MHE + +Y+ R  P W + WFF++ G C  VE  +K  +  RW+ P  ++ +L
Sbjct: 241 TFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVL 300

Query: 308 TLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFV----WKLTLSLF 340
           TL+F++VTA W F P   R       L E   +  F      KLT  LF
Sbjct: 301 TLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKLTAYLF 345

[Close]

BLAST of PI0029177.1 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.1e-54
Identity = 136/346 (39.31%), Postives = 192/346 (55.49%), Query Frame = 0


Query: 4   MYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTG 63
           M  EL S +KV +  +IS+ Y Y+ S+++  G FRL+S+ P+  LF +LPL +SS+  +G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 64  TVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYLEKY 123
           + AFF++WL  FKL+LFSF+ GPL    P     F    C PIK++              
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLF-PVPSNLSRFVCFTCFPIKLQ-------------- 120

Query: 124 ANPKLGLNLP-----AKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSA 183
            NPK    +P      K+  F +L+ + ++   + P V + +Y + +YL  + LL     
Sbjct: 121 QNPKPQNQMPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKV 180

Query: 184 IVGSAFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV---GGRN----- 243
           ++  +     LEP  NEPYL+TSLQDFWGRRWNL+V   LR  +Y PV    GR      
Sbjct: 181 LLSISL-WCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQ 240

Query: 244 ----GVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELK-TVVGRRWQ 303
               GV A F+ SGV+HE + +Y TRE PT +VT FF+L GVC   E   K T + RRW+
Sbjct: 241 ALFLGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWK 300

Query: 304 PPTVVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFV 332
              +V+ LLT+ F+V+T  W FFP L R+G    L  E  +   FV
Sbjct: 301 VSQMVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFV 330

[Close]

BLAST of PI0029177.1 vs. ExPASy Swiss-Prot
Match: Q9FGN1 (Probable long-chain-alcohol O-fatty-acyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=At5g51420 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 5.9e-53
Identity = 138/360 (38.33%), Postives = 201/360 (55.83%), Query Frame = 0


Query: 4   MYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTG 63
           M  ELK+ +KVS++++IS+ Y Y++ +++  G FR +S+ PI  LF +LPL  +S+ L+G
Sbjct: 1   MEEELKNLIKVSVSVIISISYCYYVPTRIKPGIFRFLSVLPICALFRVLPLFFASVHLSG 60

Query: 64  TVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYLEKY 123
             A FI+WL  FKL+LFSFN GPL    P     F   AC PIK++      DY +  +Y
Sbjct: 61  YTALFISWLANFKLILFSFNQGPLF-PLPSNLTRFICFACFPIKLQQNPKRRDYFFQWEY 120

Query: 124 ANPKLGLN-LPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIVGS 183
               L  N    K++I ++++ + +HI  ++P V   +Y + LYL ++ LL + +A    
Sbjct: 121 PIEVLFSNQFVTKVVILSVVLHMYNHIQHIYPIVLFVLYPLHLYLVLEILLKLFNAFFSI 180

Query: 184 AFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV-----GGRN------- 243
           A D   LEP  NEPYLA SL+DFWG  W L++   L   +Y  +     G  N       
Sbjct: 181 ALD-CELEPQLNEPYLAYSLRDFWGHWWTLMLPTILLPDVYARMRRITEGKMNSENALYL 240

Query: 244 GVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVV-EFELKTVVGRRWQP--- 303
           GV   F+ SG +HE L +YITRE PT +VT FF+L GVC+V  +  LK  +  RW     
Sbjct: 241 GVFVTFLVSGALHEFLFFYITRERPTGEVTLFFVLHGVCIVAYDARLKKKIA-RWIGFEF 300

Query: 304 -PTVVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFVWKL---TLSLFRKY 343
            P ++  ++ + F+VVTA W FFPPL+R G       E  +   FV  L   +L   RK+
Sbjct: 301 CPCLILQVMVMGFVVVTAGWLFFPPLVRTGKIQRFANEALLFIGFVRNLFNESLFFMRKF 357

[Close]

BLAST of PI0029177.1 vs. ExPASy TrEMBL
Match: A0A0A0K576 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G179120 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.1e-166
Identity = 307/354 (86.72%), Postives = 321/354 (90.68%), Query Frame = 0


Query: 1   MVVMYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIF 60
           M VM GELKSF KVSLTIM+SLLYSYFISSKLPKGKFRLISLFP F LFALLPLSLSS+F
Sbjct: 1   MAVMDGELKSFTKVSLTIMVSLLYSYFISSKLPKGKFRLISLFPTFSLFALLPLSLSSVF 60

Query: 61  LTGTVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYL 120
           L+GTVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLF ++ACLPIKIKP++IDPDYKYL
Sbjct: 61  LSGTVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFVTVACLPIKIKPREIDPDYKYL 120

Query: 121 EKYANPKLGLNLPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIV 180
           EKYANPKLGLNLPAKIL+FAILIA  DHIDQ  PNVKICVYCVTLYLFV+FLLGVSSAIV
Sbjct: 121 EKYANPKLGLNLPAKILLFAILIAAGDHIDQFRPNVKICVYCVTLYLFVEFLLGVSSAIV 180

Query: 181 GSAFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRP----VGG-------- 240
            S+FDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRP    VGG        
Sbjct: 181 RSSFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPVRAAVGGVVGKRWAA 240

Query: 241 RNGVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELKTVVGRRWQPPT 300
             GVMAVFV SGVMHEQLVYYITREVPTW+VTWFF+LQGVCVV+EFELKTVVGRRWQPP 
Sbjct: 241 GTGVMAVFVVSGVMHEQLVYYITREVPTWEVTWFFVLQGVCVVLEFELKTVVGRRWQPPA 300

Query: 301 VVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFVWKLTLSLFRKY 343
            VAWLLTLSFLVVTASW FFPPLMRAG FAGLLKELKMVE  VWKL LSLF+KY
Sbjct: 301 AVAWLLTLSFLVVTASWLFFPPLMRAGIFAGLLKELKMVERLVWKLILSLFQKY 354

[Close]

BLAST of PI0029177.1 vs. ExPASy TrEMBL
Match: A0A5A7U1M1 (Putative long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G002890 PE=3 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 8.2e-159
Identity = 296/352 (84.09%), Postives = 313/352 (88.92%), Query Frame = 0


Query: 1   MVVMYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIF 60
           MVV+ GELK FMKVSLTIM+SLLYSYFISSKLPKGKFRLISLFP+FFLFALLPLSLSS+F
Sbjct: 1   MVVVDGELKRFMKVSLTIMVSLLYSYFISSKLPKGKFRLISLFPVFFLFALLPLSLSSVF 60

Query: 61  LTGTVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYL 120
           LTGT+AFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFAS+ACLPIKIKPK+IDP+YKY 
Sbjct: 61  LTGTIAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASVACLPIKIKPKEIDPNYKYP 120

Query: 121 EKYANPKLGLNLPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIV 180
           EKY NPK    LP KIL+FAILIA +DHIDQL PNVKIC YC+TLYLFVDFLLGVSSAIV
Sbjct: 121 EKYVNPK----LPTKILLFAILIAANDHIDQLRPNVKICFYCLTLYLFVDFLLGVSSAIV 180

Query: 181 GSAFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV------------GG 240
           GSAFDGVVLEP SNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV              
Sbjct: 181 GSAFDGVVLEPPSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPVRAAVDGVVGKRWAA 240

Query: 241 RNGVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELKTVVGRRWQPPT 300
             GVMAVFVASGVMHEQLVYYI+REVPTW+VTWFF+L GVCVV+EFELK VVG RWQPP 
Sbjct: 241 GTGVMAVFVASGVMHEQLVYYISREVPTWEVTWFFVLHGVCVVLEFELKRVVGGRWQPPA 300

Query: 301 VVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFVWKLTLSLFR 341
           VVAWLLTLSF+VVTASW FFP LMRA  FA LLKELKMVEEFVWKL LSLF+
Sbjct: 301 VVAWLLTLSFMVVTASWLFFPQLMRADIFAVLLKELKMVEEFVWKLILSLFQ 348

[Close]

BLAST of PI0029177.1 vs. ExPASy TrEMBL
Match: A0A1S3BIW5 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo OX=3656 GN=LOC103490377 PE=3 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 8.2e-159
Identity = 296/352 (84.09%), Postives = 313/352 (88.92%), Query Frame = 0


Query: 1   MVVMYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIF 60
           MVV+ GELK FMKVSLTIM+SLLYSYFISSKLPKGKFRLISLFP+FFLFALLPLSLSS+F
Sbjct: 1   MVVVDGELKRFMKVSLTIMVSLLYSYFISSKLPKGKFRLISLFPVFFLFALLPLSLSSVF 60

Query: 61  LTGTVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYL 120
           LTGT+AFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFAS+ACLPIKIKPK+IDP+YKY 
Sbjct: 61  LTGTIAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASVACLPIKIKPKEIDPNYKYP 120

Query: 121 EKYANPKLGLNLPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIV 180
           EKY NPK    LP KIL+FAILIA +DHIDQL PNVKIC YC+TLYLFVDFLLGVSSAIV
Sbjct: 121 EKYVNPK----LPTKILLFAILIAANDHIDQLRPNVKICFYCLTLYLFVDFLLGVSSAIV 180

Query: 181 GSAFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV------------GG 240
           GSAFDGVVLEP SNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV              
Sbjct: 181 GSAFDGVVLEPPSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPVRAAVDGVVGKRWAA 240

Query: 241 RNGVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELKTVVGRRWQPPT 300
             GVMAVFVASGVMHEQLVYYI+REVPTW+VTWFF+L GVCVV+EFELK VVG RWQPP 
Sbjct: 241 GTGVMAVFVASGVMHEQLVYYISREVPTWEVTWFFVLHGVCVVLEFELKRVVGGRWQPPA 300

Query: 301 VVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFVWKLTLSLFR 341
           VVAWLLTLSF+VVTASW FFP LMRA  FA LLKELKMVEEFVWKL LSLF+
Sbjct: 301 VVAWLLTLSFMVVTASWLFFPQLMRADIFAVLLKELKMVEEFVWKLILSLFQ 348

[Close]

BLAST of PI0029177.1 vs. ExPASy TrEMBL
Match: A0A6J1FCN8 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita moschata OX=3662 GN=LOC111442895 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.5e-131
Identity = 245/343 (71.43%), Postives = 277/343 (80.76%), Query Frame = 0


Query: 4   MYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTG 63
           M GELKSF+KV ++IM SLLYSYFI+SK+PKGKFR  SL PIF LFA+LPLSLS++FLTG
Sbjct: 1   MEGELKSFIKVLISIMASLLYSYFIASKIPKGKFRFFSLLPIFSLFAVLPLSLSTVFLTG 60

Query: 64  TVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYLEKY 123
           TV FFITWLTT+KLLLFSFNLGPLVSDPPL FPLFASIACLPIKI PKK DPD+K L+K 
Sbjct: 61  TVTFFITWLTTYKLLLFSFNLGPLVSDPPLKFPLFASIACLPIKIMPKKTDPDHKNLKKV 120

Query: 124 ANPKLGLNLPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIVGSA 183
            NPKL LNLPAKI+IFA  IA  DHID+L PN KICVY V LY+FVD LLGV S      
Sbjct: 121 PNPKLPLNLPAKIVIFAGFIAAGDHIDRLRPNAKICVYSVLLYIFVDILLGVPSVFFRLM 180

Query: 184 FDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV------------GGRNG 243
           F GV LEP SNEPYL TSLQDFWGRRWNLLVSN+LRY +Y+PV                 
Sbjct: 181 FGGVELEPGSNEPYLMTSLQDFWGRRWNLLVSNSLRYSVYKPVRAAVEGLIGPTWAAGTA 240

Query: 244 VMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELKTVVGRRWQPPTVVA 303
           VMAVFV SGVMHEQ+VYY+TRE PTW+ TWFF++QGVCVVVE  LK  VGRRW+PP+VVA
Sbjct: 241 VMAVFVVSGVMHEQMVYYLTREFPTWETTWFFVVQGVCVVVESGLKAAVGRRWRPPSVVA 300

Query: 304 WLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFVWKL 335
            LLTL+ L+VTA+W FFPP ++AGTFA LL+ELKMV +F+WKL
Sbjct: 301 GLLTLAVLMVTAAWLFFPPFLKAGTFASLLRELKMVGKFLWKL 343

[Close]

BLAST of PI0029177.1 vs. ExPASy TrEMBL
Match: A0A6J1CCA4 (LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Momordica charantia OX=3673 GN=LOC111010365 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 1.1e-99
Identity = 208/348 (59.77%), Postives = 245/348 (70.40%), Query Frame = 0


Query: 4   MYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTG 63
           M  EL + +KVSL +  SLLYSYF++SKLPKGKFRL+SL PIF LFALLP  LS     G
Sbjct: 1   MAAELHALIKVSLCVFASLLYSYFVASKLPKGKFRLLSLLPIFPLFALLPXLLS-----G 60

Query: 64  TVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIK---PKKIDPDYKYL 123
            VAFFITWL +FKLL FSF+ GPL SDPPL FPLFASIAC PI+IK   PK + P     
Sbjct: 61  IVAFFITWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLP 120

Query: 124 EKYANPKLGLNLPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIV 183
           +   +PKL LNLPAK L+FA L+A DD    L  N K+C YC  LY F+D +LG+S+AIV
Sbjct: 121 D---SPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIV 180

Query: 184 GSAFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV----GGRNG----- 243
            SAF G+ LE  SNEPYLATSLQDFWGRRWNLLVSNTLRY +YRPV    G R G     
Sbjct: 181 RSAF-GIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYXVYRPVRAALGRRRGAAPAA 240

Query: 244 VMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELKTVVGRRWQPPTVVA 303
           V A FV SG+MHE L YY+TR VP W+VT FF+L GVCVVVEF +K  VG +W+    V+
Sbjct: 241 VFATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRLHWAVS 300

Query: 304 WLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFVWKLTLSLF 340
             LT++F+VVT +W FFPPL+R G   G+L+E K   EFV     S+F
Sbjct: 301 GPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVKAQVSSVF 339

[Close]

BLAST of PI0029177.1 vs. NCBI nr
Match: XP_004151617.2 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis sativus] >KGN44084.1 hypothetical protein Csa_015517 [Cucumis sativus])

HSP 1 Score: 594.0 bits (1530), Expect = 8.4e-166
Identity = 307/354 (86.72%), Postives = 321/354 (90.68%), Query Frame = 0


Query: 1   MVVMYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIF 60
           M VM GELKSF KVSLTIM+SLLYSYFISSKLPKGKFRLISLFP F LFALLPLSLSS+F
Sbjct: 1   MAVMDGELKSFTKVSLTIMVSLLYSYFISSKLPKGKFRLISLFPTFSLFALLPLSLSSVF 60

Query: 61  LTGTVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYL 120
           L+GTVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLF ++ACLPIKIKP++IDPDYKYL
Sbjct: 61  LSGTVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFVTVACLPIKIKPREIDPDYKYL 120

Query: 121 EKYANPKLGLNLPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIV 180
           EKYANPKLGLNLPAKIL+FAILIA  DHIDQ  PNVKICVYCVTLYLFV+FLLGVSSAIV
Sbjct: 121 EKYANPKLGLNLPAKILLFAILIAAGDHIDQFRPNVKICVYCVTLYLFVEFLLGVSSAIV 180

Query: 181 GSAFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRP----VGG-------- 240
            S+FDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRP    VGG        
Sbjct: 181 RSSFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPVRAAVGGVVGKRWAA 240

Query: 241 RNGVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELKTVVGRRWQPPT 300
             GVMAVFV SGVMHEQLVYYITREVPTW+VTWFF+LQGVCVV+EFELKTVVGRRWQPP 
Sbjct: 241 GTGVMAVFVVSGVMHEQLVYYITREVPTWEVTWFFVLQGVCVVLEFELKTVVGRRWQPPA 300

Query: 301 VVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFVWKLTLSLFRKY 343
            VAWLLTLSFLVVTASW FFPPLMRAG FAGLLKELKMVE  VWKL LSLF+KY
Sbjct: 301 AVAWLLTLSFLVVTASWLFFPPLMRAGIFAGLLKELKMVERLVWKLILSLFQKY 354

[Close]

BLAST of PI0029177.1 vs. NCBI nr
Match: XP_008448090.1 (PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo] >KAA0048137.1 putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa] >TYK08117.1 putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 569.7 bits (1467), Expect = 1.7e-158
Identity = 296/352 (84.09%), Postives = 313/352 (88.92%), Query Frame = 0


Query: 1   MVVMYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIF 60
           MVV+ GELK FMKVSLTIM+SLLYSYFISSKLPKGKFRLISLFP+FFLFALLPLSLSS+F
Sbjct: 1   MVVVDGELKRFMKVSLTIMVSLLYSYFISSKLPKGKFRLISLFPVFFLFALLPLSLSSVF 60

Query: 61  LTGTVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYL 120
           LTGT+AFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFAS+ACLPIKIKPK+IDP+YKY 
Sbjct: 61  LTGTIAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASVACLPIKIKPKEIDPNYKYP 120

Query: 121 EKYANPKLGLNLPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIV 180
           EKY NPK    LP KIL+FAILIA +DHIDQL PNVKIC YC+TLYLFVDFLLGVSSAIV
Sbjct: 121 EKYVNPK----LPTKILLFAILIAANDHIDQLRPNVKICFYCLTLYLFVDFLLGVSSAIV 180

Query: 181 GSAFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV------------GG 240
           GSAFDGVVLEP SNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV              
Sbjct: 181 GSAFDGVVLEPPSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPVRAAVDGVVGKRWAA 240

Query: 241 RNGVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELKTVVGRRWQPPT 300
             GVMAVFVASGVMHEQLVYYI+REVPTW+VTWFF+L GVCVV+EFELK VVG RWQPP 
Sbjct: 241 GTGVMAVFVASGVMHEQLVYYISREVPTWEVTWFFVLHGVCVVLEFELKRVVGGRWQPPA 300

Query: 301 VVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFVWKLTLSLFR 341
           VVAWLLTLSF+VVTASW FFP LMRA  FA LLKELKMVEEFVWKL LSLF+
Sbjct: 301 VVAWLLTLSFMVVTASWLFFPQLMRADIFAVLLKELKMVEEFVWKLILSLFQ 348

[Close]

BLAST of PI0029177.1 vs. NCBI nr
Match: XP_038899806.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Benincasa hispida])

HSP 1 Score: 559.3 bits (1440), Expect = 2.3e-155
Identity = 286/348 (82.18%), Postives = 307/348 (88.22%), Query Frame = 0


Query: 4   MYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTG 63
           M GELKSF+KVSLTIM+SLLYSYFI+SK+PKGKFRL SLFP+FFLFALLP SLSSIFLTG
Sbjct: 1   MDGELKSFIKVSLTIMVSLLYSYFIASKIPKGKFRLFSLFPVFFLFALLPPSLSSIFLTG 60

Query: 64  TVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYLEKY 123
           TVAFFITWLTTFKL LFSFNLGPLVSDPPLTFPLFAS+ACLPIKIKPK+ D DYKYL+KY
Sbjct: 61  TVAFFITWLTTFKLFLFSFNLGPLVSDPPLTFPLFASVACLPIKIKPKETDQDYKYLKKY 120

Query: 124 ANPKLGLNLPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIVGSA 183
           +NPKLGLNLPAKIL+FAILIA+DD ID+L PN K+CVYC TLYLF+D LLGVSSA V  A
Sbjct: 121 SNPKLGLNLPAKILLFAILIAIDDRIDRLRPNAKLCVYCATLYLFLDVLLGVSSAFVRLA 180

Query: 184 FDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV------------GGRNG 243
           FD VVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV                G
Sbjct: 181 FDNVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPVRAAVEGVVGKRLAAGTG 240

Query: 244 VMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELKTVVGRRWQPPTVVA 303
           VMAVFVASGVMHEQLVYYITREVPTW+VTWFF+LQGV VVVE ELK VVGR W+PP VVA
Sbjct: 241 VMAVFVASGVMHEQLVYYITREVPTWEVTWFFVLQGVSVVVESELKAVVGRWWRPPAVVA 300

Query: 304 WLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFVWKLTLSLF 340
           WLLTL+FLVVTA+W FFPPL+RAG FAGLL+ELKMVEEF WKL L LF
Sbjct: 301 WLLTLTFLVVTAAWLFFPPLLRAGIFAGLLRELKMVEEFAWKLVLRLF 348

[Close]

BLAST of PI0029177.1 vs. NCBI nr
Match: XP_022936223.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita moschata])

HSP 1 Score: 479.2 bits (1232), Expect = 3.0e-131
Identity = 245/343 (71.43%), Postives = 277/343 (80.76%), Query Frame = 0


Query: 4   MYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTG 63
           M GELKSF+KV ++IM SLLYSYFI+SK+PKGKFR  SL PIF LFA+LPLSLS++FLTG
Sbjct: 1   MEGELKSFIKVLISIMASLLYSYFIASKIPKGKFRFFSLLPIFSLFAVLPLSLSTVFLTG 60

Query: 64  TVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYLEKY 123
           TV FFITWLTT+KLLLFSFNLGPLVSDPPL FPLFASIACLPIKI PKK DPD+K L+K 
Sbjct: 61  TVTFFITWLTTYKLLLFSFNLGPLVSDPPLKFPLFASIACLPIKIMPKKTDPDHKNLKKV 120

Query: 124 ANPKLGLNLPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIVGSA 183
            NPKL LNLPAKI+IFA  IA  DHID+L PN KICVY V LY+FVD LLGV S      
Sbjct: 121 PNPKLPLNLPAKIVIFAGFIAAGDHIDRLRPNAKICVYSVLLYIFVDILLGVPSVFFRLM 180

Query: 184 FDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV------------GGRNG 243
           F GV LEP SNEPYL TSLQDFWGRRWNLLVSN+LRY +Y+PV                 
Sbjct: 181 FGGVELEPGSNEPYLMTSLQDFWGRRWNLLVSNSLRYSVYKPVRAAVEGLIGPTWAAGTA 240

Query: 244 VMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELKTVVGRRWQPPTVVA 303
           VMAVFV SGVMHEQ+VYY+TRE PTW+ TWFF++QGVCVVVE  LK  VGRRW+PP+VVA
Sbjct: 241 VMAVFVVSGVMHEQMVYYLTREFPTWETTWFFVVQGVCVVVESGLKAAVGRRWRPPSVVA 300

Query: 304 WLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFVWKL 335
            LLTL+ L+VTA+W FFPP ++AGTFA LL+ELKMV +F+WKL
Sbjct: 301 GLLTLAVLMVTAAWLFFPPFLKAGTFASLLRELKMVGKFLWKL 343

[Close]

BLAST of PI0029177.1 vs. NCBI nr
Match: KAG6592068.1 (Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 475.3 bits (1222), Expect = 4.4e-130
Identity = 244/343 (71.14%), Postives = 275/343 (80.17%), Query Frame = 0


Query: 4   MYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTG 63
           M GELKSF+KV ++IM SLLYSYFI+SK+PKGKFR  SL PIF LFA+LPLSLS++FLTG
Sbjct: 1   MEGELKSFIKVLISIMASLLYSYFIASKIPKGKFRFFSLLPIFSLFAVLPLSLSTVFLTG 60

Query: 64  TVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYLEKY 123
           TV FFITWLTT+KLLLFSFNLGPLVSDPPL FPLFASIACLPIKIKPKK DPD+K L+K 
Sbjct: 61  TVTFFITWLTTYKLLLFSFNLGPLVSDPPLKFPLFASIACLPIKIKPKKTDPDHKNLKKI 120

Query: 124 ANPKLGLNLPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIVGSA 183
            NPKL LNLPAKI+IFA  IA  DHID+L PN KICVY V LY FVD LLGV S      
Sbjct: 121 PNPKLPLNLPAKIVIFAGFIAAGDHIDRLRPNAKICVYSVLLYFFVDILLGVPSVFFQLM 180

Query: 184 FDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV------------GGRNG 243
           F GV LEP SNEPYL TSLQDFWGRRWNLLVSN+LRY +Y+PV                 
Sbjct: 181 FGGVELEPGSNEPYLMTSLQDFWGRRWNLLVSNSLRYSVYKPVQEAVEGLIGPTWAAGVA 240

Query: 244 VMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELKTVVGRRWQPPTVVA 303
           VMAVFV SGVMHEQ+VYY+TRE PTW+ TWF ++QGVCVVVE  LK  VGRRW+PP+VVA
Sbjct: 241 VMAVFVVSGVMHEQMVYYLTREFPTWETTWFLVVQGVCVVVESGLKAAVGRRWRPPSVVA 300

Query: 304 WLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFVWKL 335
            LLTL+ L+VTA+W FFPP ++AGTF  LL+ELKMV +F+WKL
Sbjct: 301 GLLTLAVLMVTAAWLFFPPFLKAGTFESLLRELKMVGKFLWKL 343

[Close]

BLAST of PI0029177.1 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 225.3 bits (573), Expect = 7.3e-59
Identity = 144/343 (41.98%), Postives = 207/343 (60.35%), Query Frame = 0


Query: 4   MYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTG 63
           M  ELK+F+K+ ++ +IS+ Y Y++S+ +  G FRL+S+ P+  LF + PL  S +  +G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 64  TVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYLEKY 123
            +AFF++WL  FKL+LFSF+ GPL S  P T   F  I C P  IKP++ +P+ +     
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL-SPLPRTLSRFICITCFP--IKPQQ-NPNIQ----- 120

Query: 124 ANPKLGLNLPA-KILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIVGS 183
            N K+ + L A K++IF +L+ + ++   L P + +    + ++L ++ +  +  A+V  
Sbjct: 121 -NYKIPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFI 180

Query: 184 AFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPVGGRN------------ 243
              G  LEP SNEPYLATSLQDFWGRRWNL+V   LR  +Y P                 
Sbjct: 181 TL-GCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFL 240

Query: 244 GVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELK--TVVGRRWQPPT 303
           GV A F+ SG +HE L +Y+TREVPT +VTWFF+L GVC V E  +K  T V R W+   
Sbjct: 241 GVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSP 300

Query: 304 VVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFV 332
            V+ LLT+ F+VVT+ WFFF PL+R+G    L  E  M  +FV
Sbjct: 301 TVSRLLTVGFVVVTSGWFFF-PLIRSGIIERLASEALMCIDFV 331

[Close]

BLAST of PI0029177.1 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 224.2 bits (570), Expect = 1.6e-58
Identity = 138/344 (40.12%), Postives = 197/344 (57.27%), Query Frame = 0


Query: 7   ELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTGTVA 66
           ELK+ +KV ++ +IS+ Y Y+I  ++  G  R +S+ P+  LF +LPL  SS+ L+   A
Sbjct: 4   ELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITA 63

Query: 67  FFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYLEKYANP 126
           FF+TWL  FKL+LFSF+ GPL+   P  FP F    C PIK++               NP
Sbjct: 64  FFLTWLANFKLILFSFDKGPLI-PIPTNFPRFFCFTCFPIKVQ--------------QNP 123

Query: 127 KLGLNLP-----AKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIVG 186
           K   +LP      K+ IFA+L+ +  +   L P + + +Y V LYL ++ +L     +V 
Sbjct: 124 KSQNHLPKLVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVF 183

Query: 187 SAFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRP---VGGRN-------- 246
            +  G  LEP SN+PYLATSLQDFWGRRWNL+V   LR  +Y P   V  R         
Sbjct: 184 ISL-GCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 243

Query: 247 -GVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFEL--KTVVGRRWQPP 306
            G++A F+ SG++HE L +Y+ RE+PT +VT FF+L GVC  VE  +  KT V +RW+  
Sbjct: 244 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 303

Query: 307 TVVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFV 332
             V+ +LT+ F+ VT  W F P L R+G       E  ++ EF+
Sbjct: 304 PGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFI 331

[Close]

BLAST of PI0029177.1 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 217.2 bits (552), Expect = 2.0e-56
Identity = 132/349 (37.82%), Postives = 193/349 (55.30%), Query Frame = 0


Query: 8   LKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTGTVAF 67
           + SF+K    ++ISL Y++FI+  +PKG  RLI  FP+F +F ++P  + S+ L G  AF
Sbjct: 1   MASFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAF 60

Query: 68  FITWLTTFKLLLFSFNLGPLVSD-PPLTFPLFASIACLPIKIKPKKIDPDYKYLEKYANP 127
           FI WL  FKLLLF+   GPL S+  PL+ P+F +++CLPIKI   ++ P       +   
Sbjct: 61  FIAWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKI---QLSPKPTKTHSHEGS 120

Query: 128 KLG-LNLPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIVGSAFD 187
             G L    K +   ++I   ++  +L   V + +Y + +Y  ++ +L  ++A V  A  
Sbjct: 121 TEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAV-RAMS 180

Query: 188 GVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRP------VGGRN-----GVMA 247
            + LEP  N+PYLATSLQDFWGRRWNL+V+  LR  +Y P      V G N         
Sbjct: 181 DLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLGPNYSQILAAFG 240

Query: 248 VFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELKTVVGRRWQPPTVVAWLL 307
            FV SG+MHE + +Y+ R  P W + WFF++ G C  VE  +K  +  RW+ P  ++ +L
Sbjct: 241 TFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVL 300

Query: 308 TLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFV----WKLTLSLF 340
           TL+F++VTA W F P   R       L E   +  F      KLT  LF
Sbjct: 301 TLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKLTAYLF 345

[Close]

BLAST of PI0029177.1 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 213.8 bits (543), Expect = 2.2e-55
Identity = 136/346 (39.31%), Postives = 192/346 (55.49%), Query Frame = 0


Query: 4   MYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTG 63
           M  EL S +KV +  +IS+ Y Y+ S+++  G FRL+S+ P+  LF +LPL +SS+  +G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 64  TVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYLEKY 123
           + AFF++WL  FKL+LFSF+ GPL    P     F    C PIK++              
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLF-PVPSNLSRFVCFTCFPIKLQ-------------- 120

Query: 124 ANPKLGLNLP-----AKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSA 183
            NPK    +P      K+  F +L+ + ++   + P V + +Y + +YL  + LL     
Sbjct: 121 QNPKPQNQMPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKV 180

Query: 184 IVGSAFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV---GGRN----- 243
           ++  +     LEP  NEPYL+TSLQDFWGRRWNL+V   LR  +Y PV    GR      
Sbjct: 181 LLSISL-WCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQ 240

Query: 244 ----GVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVVEFELK-TVVGRRWQ 303
               GV A F+ SGV+HE + +Y TRE PT +VT FF+L GVC   E   K T + RRW+
Sbjct: 241 ALFLGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWK 300

Query: 304 PPTVVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFV 332
              +V+ LLT+ F+V+T  W FFP L R+G    L  E  +   FV
Sbjct: 301 VSQMVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFV 330

[Close]

BLAST of PI0029177.1 vs. TAIR 10
Match: AT5G51420.1 (long-chain-alcohol O-fatty-acyltransferase family protein / wax synthase family protein )

HSP 1 Score: 209.5 bits (532), Expect = 4.2e-54
Identity = 138/360 (38.33%), Postives = 201/360 (55.83%), Query Frame = 0


Query: 4   MYGELKSFMKVSLTIMISLLYSYFISSKLPKGKFRLISLFPIFFLFALLPLSLSSIFLTG 63
           M  ELK+ +KVS++++IS+ Y Y++ +++  G FR +S+ PI  LF +LPL  +S+ L+G
Sbjct: 1   MEEELKNLIKVSVSVIISISYCYYVPTRIKPGIFRFLSVLPICALFRVLPLFFASVHLSG 60

Query: 64  TVAFFITWLTTFKLLLFSFNLGPLVSDPPLTFPLFASIACLPIKIKPKKIDPDYKYLEKY 123
             A FI+WL  FKL+LFSFN GPL    P     F   AC PIK++      DY +  +Y
Sbjct: 61  YTALFISWLANFKLILFSFNQGPLF-PLPSNLTRFICFACFPIKLQQNPKRRDYFFQWEY 120

Query: 124 ANPKLGLN-LPAKILIFAILIAVDDHIDQLWPNVKICVYCVTLYLFVDFLLGVSSAIVGS 183
               L  N    K++I ++++ + +HI  ++P V   +Y + LYL ++ LL + +A    
Sbjct: 121 PIEVLFSNQFVTKVVILSVVLHMYNHIQHIYPIVLFVLYPLHLYLVLEILLKLFNAFFSI 180

Query: 184 AFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPV-----GGRN------- 243
           A D   LEP  NEPYLA SL+DFWG  W L++   L   +Y  +     G  N       
Sbjct: 181 ALD-CELEPQLNEPYLAYSLRDFWGHWWTLMLPTILLPDVYARMRRITEGKMNSENALYL 240

Query: 244 GVMAVFVASGVMHEQLVYYITREVPTWDVTWFFILQGVCVVV-EFELKTVVGRRWQP--- 303
           GV   F+ SG +HE L +YITRE PT +VT FF+L GVC+V  +  LK  +  RW     
Sbjct: 241 GVFVTFLVSGALHEFLFFYITRERPTGEVTLFFVLHGVCIVAYDARLKKKIA-RWIGFEF 300

Query: 304 -PTVVAWLLTLSFLVVTASWFFFPPLMRAGTFAGLLKELKMVEEFVWKL---TLSLFRKY 343
            P ++  ++ + F+VVTA W FFPPL+R G       E  +   FV  L   +L   RK+
Sbjct: 301 CPCLILQVMVMGFVVVTAGWLFFPPLVRTGKIQRFANEALLFIGFVRNLFNESLFFMRKF 357

The following BLAST results are available for this feature:

BLAST of PI0029177.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJ74	1.0e-57	41.98	Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX...	[more]
Q9FJ76	2.3e-57	40.12	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9SV07	2.8e-55	37.82	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9FJ75	3.1e-54	39.31	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FGN1	5.9e-53	38.33	Probable long-chain-alcohol O-fatty-acyltransferase 11 OS=Arabidopsis thaliana O...	[more]

BLAST of PI0029177.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K576	4.1e-166	86.72	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G179120 PE=...	[more]
A0A5A7U1M1	8.2e-159	84.09	Putative long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo var. makuw...	[more]
A0A1S3BIW5	8.2e-159	84.09	probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo OX=3656 GN...	[more]
A0A6J1FCN8	1.5e-131	71.43	probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita moschata OX=3...	[more]
A0A6J1CCA4	1.1e-99	59.77	LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Mo...	[more]

BLAST of PI0029177.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004151617.2	8.4e-166	86.72	probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis sativus] >KGN4408...	[more]
XP_008448090.1	1.7e-158	84.09	PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo] ...	[more]
XP_038899806.1	2.3e-155	82.18	probable long-chain-alcohol O-fatty-acyltransferase 5 [Benincasa hispida]	[more]
XP_022936223.1	3.0e-131	71.43	probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita moschata]	[more]
KAG6592068.1	4.4e-130	71.14	Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sor...	[more]

BLAST of PI0029177.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55360.1	7.3e-59	41.98	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55340.1	1.6e-58	40.12	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT3G51970.1	2.0e-56	37.82	acyl-CoA sterol acyl transferase 1	[more]
AT5G55350.1	2.2e-55	39.31	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G51420.1	4.2e-54	38.33	long-chain-alcohol O-fatty-acyltransferase family protein / wax synthase family ...	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 191..264 e-value: 4.2E-10 score: 39.8
None	No IPR available	PANTHER	PTHR31595:SF8	(MEMBRANE BOUND O-ACYL TRANSFERASE) FAMILY PROTEIN, PUTATIVE-RELATED	coord: 4..337
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 4..337

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
PI0029177	PI0029177	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0029177.1-exon	PI0029177.1-exon-chr01:12367327..12368001	exon
PI0029177.1-exon	PI0029177.1-exon-chr01:12368037..12368390	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0029177.1-cds	PI0029177.1-cds-chr01:12367327..12368001	CDS
PI0029177.1-cds	PI0029177.1-cds-chr01:12368037..12368390	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
PI0029177.1	PI0029177.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity