PI0023612.1 (mRNA) Melon (PI 482460) v1

Overview
NamePI0023612.1
TypemRNA
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionprotein NRT1/ PTR FAMILY 6.2-like
Locationchr02: 18733068 .. 18737242 (+)
Sequence length2019
RNA-Seq ExpressionPI0023612.1
SyntenyPI0023612.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACAACACTCTCATTTCAAACTTCCAATATTAACCATTTTAAAACAAACTCCAACTCCTCTCTCTTACACACCCTTTCTTCTATCTCACTCACCACACATCTTCTCCATATTTTCAACCTTAAAAAATGGTTGGTCTTTTTTATTTTTAACAAAAAATAAAGACTCAAATTTTCTTCTTTAGCTTTATTTTTGTCTTATTTTAAATGGGTGTTTTTAAAAATTTGATTATTTTTCTTTTGGTTTTGGGTTAGGATGGGAAGAAGCTAAGTTGGACAGTAGCTGATGCTGTGGATTACAAAGGTTATCCAGCAGATAAAACTAAGACTGGTGGTTGGATTCCTGCTGCTCTTATTTTAGGTATCTTAATTATTTATTATTCATTATTACTTCATTAATGTATTTAATACTCCAACCTAACTAAGTATTATCGCAAATGATTAATTGTAATTTAGGTTACATCTAGTCCCATGCATTTGTCTCTGGTATACAGTAAAAAATAAAAATAAAGAAAAAAACAATTCATACATATATATATTTAAAAATTTACAACCGTCTAAACTGCACAAAGATATAGATGCACCACATGCAACTTCCAAATTTTTTTTTCTTCTTATCAAACTGTGATTTATTCTTTTTATAATTTTTGCTGATTCTATAAAGGTGTTTTCTTTCCGATATCTATTTTGTTCAAAAAGAGAATCAAGAAAAAGTTCCACTTTTTCGCTTTGAACCTTCTTTAATTATTTAATAAACACAACTAAATTAACATTTTCATATTGTGCATATAATTAACAAATAATATTTCAAATTACTCTCTCCTTTTATTTCACACAATAATATAAAAATAGCTTTAAAAAAAAATTTATTCTACGTATTATGACAGTCCTAGAAATTTTTCGGTTACGTTGTTTCTTTCTCAGTGTTTTTCCCGAAAAAATAATAGTGATGAAATTTTTTTGCTGAAAAAATAGAAAGTAGGCAGAGATTCCTTTAAGATTCGTTTTGGGAGGTTTGTTTTTTTGGTTTTTCCATGAAGGTTTTGATCAGCAGATTCTGTGCCAACAACAAAACTCCAAATTTAAAAAGGAGAATAATTGAGAGGGACGTACGTGTATAGTATAGATAGCAGATTAGTGACGTTTTTTTTCTCCTTTTTTGTGAGTATATGGGAATTTAATGACACAATCTTATGTCACTTCATCACAATCAATAATTATTCAATGTTTCATGTGGCCTTTTTTATCACTTTGTTTTCCGTTGTAGATTAAATTTATAAGTATATGTTTAAGAAATTGTTGACAAAGATTTAGTTGGATAAATGATTGACTACTGATTAGATAATTCTAAAACATGACTATGTAATCTTTTTTCTTAATATTAGATCACAATTCTTTTAAACCAAAATTATATAAACAAGTTGTGCATGTAAACTTGAATTTTGCTTAAAAAAGAACTTAGGACATCGTGAAGAAATGAAATTGTTTAGTTTGGAGAATTGTTAAGAGAATTAATTAGTTAGGAAGTGTATGGTATAAAATATTAATAAGTTATAAAAATTGATTTTGTTTTTATTTGTGGGACCTACAAAATATTCGGACCAAACAGATAGTGGAATTACCCTTACTGTGTTTTTTCTTTTAGGCCAACATAAAGTACCTTTTTCTTGTTCTATATATATACACCTTATTTTTATTTTGTTTTTTTTTTATTAATATTTAAATCACAAACCTCCTAAGCCTCGAGAAGATGGTAAAGAAAATAAGTAATTGAAACTTTGCTCAAAGATATTACTTAAGTAGCGTCTGATACAAAAAGGAGACTAAAAGTCAAATAATGTTTTTTTTAATCGTGGGACCTACAAACTTGGTTAACTCTCCTCCTCCTTGGGCCAAATATGGCCATTATAAAATAGTTATTTTCCTATTCTCATTTCTATTACTTCAAACAAAACCAAAACTCCTACTTTGGAATTTCTTTAAAAAAGGAAAAAGTGACAGTAGTTATTAGTAACAAATATTGAATCATAAGAAGTATTAAGCTTTACATAAGTTAGTTTTTAATTTACAGGTATTGAAATATGTGAAAGGCTGTCAACAATGGGAATAGCAGTGAATCTTGTGACTTATTTGGGCGGGGTAATGCATTTGCCCAGTGCAGCTTCAGCCAACATTGTTACAGATTTCATGGGAACTTGCTTTCTCCTCTGTTTGCTTGGAGGCTTTCTTGCTGATTCTTTCCTTGGTCGATATTACACCATTCTCATCTCTGCCATCATTCAAACACTGGTACATGTTCATCAAACTAAAAATTCAATTCATGAACTTGTCATATAATTAAAGATAGTAAGTAACAATGGTTTAAATTTTGGCACTTTAGGGGACTGCTGCACTTGCAGTTTCAACAAAACTACCACAGCTTCGTCCGCCGCCATGCAATGGCACCAAAACCTGCAAGGAAGCAAATGGATTTCAAATGGGAATTTTGTATCTAGCTTTGTATCTCATTGCTTTAGGGACTGGTGGCTTAAAATCAAGCATTTCGGGTTTCGGAACTGATCAGTTTGATGATACAGATGAGAAAGAAAAGGCTAAGATGGCCTATTTCTTTAACAGATTCTTCTTCTTTATCAGCACTGGGACTTTGATGGCTGTCACAGTGTTGGTTTATATTCAAGATGAAGTGGGTCGAAGTTGGGCGTATGGGATTTGTTCTGTTTCCATGCTTGGCGCGATTTTGTTGTTCCTTTCAGGAACCAAGAGGTACAGATACAAGAAGAGCATGGGGAGCCCTGTAGTTCATATTCTACAAGTCATTGCTGCTGCAATCAAGAAGAGAAAATTGGAGCTTCCTCAGAATGTTGGTTTGCTTTATGAGGATTCTCCTGATCAGGCTTCAAGAATTGAGCACACAAAACAATTCCAGTAAGATCATTGATCATCTACAATTTTCAAATGATTCAATCAATCATATTCATAGTATACAACAAACATCATGTGAAGTTCACGAAATTAATCTTTCCTCAAAAAATTCTTAAAGTATATTGATTTGTGATTCCTTTTTGTATAGATTCTTGGACAAGGCTGCAATTGTTGCAGAAGGCGATTTCGAGACGCATGGTGCTGTTTCGGCTCTAAATCCATGGAAGCTAAGCTCAGTGACGAGAGTGGAGGAGGTGAAAATGATGGCAAGATTGCTGCCAATATGGGCCACAACCATCATCTTCTGGACTACATATGCCCAAATGATCACATTCTCAGTTCAACAAGCATCCACAATGGAAAGATCAATTGGATCTTTCCAGATTCCAGCAGGCTCTCTCACTGTCTTCTTTGTAGCAGCCATATTAATAACTTTGGGTGTTTATGATCGTCTTATCATGCCTCTTTGGAAGAAATGGAAAGGAAAACAAGGTAAATTCAAATCCATCTAAATACCAATCTCTATTTGTATAGACATTTTTTCAAAGAATCCTGAATAGATATCTTCTTATGCTACAGGTTTCACAAACATACAAAGAATGGCCATAGGCCTTGTCCTATCAATACTTGGAATGGCAGCAGCGGTACTAGCGGAGAGGAAAAGGCTGGCAGTGGCGAAAGCAGTTGGTGGCGACACAACTACCCTACCTATAAGTGTGTTCTTATTGATCCCACAATTTTTCTTGGTGGGGGCAGGTGAAGCCTTCATATACACAGGGCAACTTGATTTCTTCATAACCAAATCTCCCAAAGGGATGAAAACTATGAGTACCGGTCTCTTCCTCTCAACATTATCCCTCGGGTTTTTCATCAGTAGTTTCTTGGTTTCAGTTGTGAAGAGGGTGACAGGAAACGATGGAGAAGGTTGGCTTGCAGACAATATAAATTATGGCAGACTTGACTGCTTCTATGGACTATTGACCATATTAAGTATCATAAACTTTGTTGCTTTTCTGGTATGTGTTATATGGTACATGCCACAAAAGCGCAAAGACTTTCAAAAGAATGGCATAGTCAATGGCAACTTGGCTGAAGAGAAATGCTAATGAGAACTATAAAAGAAAAACAAACCAAAGTGTTGTGTAATGTTTTGACTTTCCTTTCAATTCTCCCCTGCATTCTAAATTATCAGCTAAAGTTTGGATTGAGAATGTAACATGCTTTATGTATAAAGTATTGCCAGTTTCACTCATCA

mRNA sequence

CAACAACACTCTCATTTCAAACTTCCAATATTAACCATTTTAAAACAAACTCCAACTCCTCTCTCTTACACACCCTTTCTTCTATCTCACTCACCACACATCTTCTCCATATTTTCAACCTTAAAAAATGGATGGGAAGAAGCTAAGTTGGACAGTAGCTGATGCTGTGGATTACAAAGGTTATCCAGCAGATAAAACTAAGACTGGTGGTTGGATTCCTGCTGCTCTTATTTTAGGTATTGAAATATGTGAAAGGCTGTCAACAATGGGAATAGCAGTGAATCTTGTGACTTATTTGGGCGGGGTAATGCATTTGCCCAGTGCAGCTTCAGCCAACATTGTTACAGATTTCATGGGAACTTGCTTTCTCCTCTGTTTGCTTGGAGGCTTTCTTGCTGATTCTTTCCTTGGTCGATATTACACCATTCTCATCTCTGCCATCATTCAAACACTGGGGACTGCTGCACTTGCAGTTTCAACAAAACTACCACAGCTTCGTCCGCCGCCATGCAATGGCACCAAAACCTGCAAGGAAGCAAATGGATTTCAAATGGGAATTTTGTATCTAGCTTTGTATCTCATTGCTTTAGGGACTGGTGGCTTAAAATCAAGCATTTCGGGTTTCGGAACTGATCAGTTTGATGATACAGATGAGAAAGAAAAGGCTAAGATGGCCTATTTCTTTAACAGATTCTTCTTCTTTATCAGCACTGGGACTTTGATGGCTGTCACAGTGTTGGTTTATATTCAAGATGAAGTGGGTCGAAGTTGGGCGTATGGGATTTGTTCTGTTTCCATGCTTGGCGCGATTTTGTTGTTCCTTTCAGGAACCAAGAGGTACAGATACAAGAAGAGCATGGGGAGCCCTGTAGTTCATATTCTACAAGTCATTGCTGCTGCAATCAAGAAGAGAAAATTGGAGCTTCCTCAGAATGTTGGTTTGCTTTATGAGGATTCTCCTGATCAGGCTTCAAGAATTGAGCACACAAAACAATTCCAATTCTTGGACAAGGCTGCAATTGTTGCAGAAGGCGATTTCGAGACGCATGGTGCTGTTTCGGCTCTAAATCCATGGAAGCTAAGCTCAGTGACGAGAGTGGAGGAGGTGAAAATGATGGCAAGATTGCTGCCAATATGGGCCACAACCATCATCTTCTGGACTACATATGCCCAAATGATCACATTCTCAGTTCAACAAGCATCCACAATGGAAAGATCAATTGGATCTTTCCAGATTCCAGCAGGCTCTCTCACTGTCTTCTTTGTAGCAGCCATATTAATAACTTTGGGTGTTTATGATCGTCTTATCATGCCTCTTTGGAAGAAATGGAAAGGAAAACAAGGTTTCACAAACATACAAAGAATGGCCATAGGCCTTGTCCTATCAATACTTGGAATGGCAGCAGCGGTACTAGCGGAGAGGAAAAGGCTGGCAGTGGCGAAAGCAGTTGGTGGCGACACAACTACCCTACCTATAAGTGTGTTCTTATTGATCCCACAATTTTTCTTGGTGGGGGCAGGTGAAGCCTTCATATACACAGGGCAACTTGATTTCTTCATAACCAAATCTCCCAAAGGGATGAAAACTATGAGTACCGGTCTCTTCCTCTCAACATTATCCCTCGGGTTTTTCATCAGTAGTTTCTTGGTTTCAGTTGTGAAGAGGGTGACAGGAAACGATGGAGAAGGTTGGCTTGCAGACAATATAAATTATGGCAGACTTGACTGCTTCTATGGACTATTGACCATATTAAGTATCATAAACTTTGTTGCTTTTCTGGTATGTGTTATATGGTACATGCCACAAAAGCGCAAAGACTTTCAAAAGAATGGCATAGTCAATGGCAACTTGGCTGAAGAGAAATGCTAATGAGAACTATAAAAGAAAAACAAACCAAAGTGTTGTGTAATGTTTTGACTTTCCTTTCAATTCTCCCCTGCATTCTAAATTATCAGCTAAAGTTTGGATTGAGAATGTAACATGCTTTATGTATAAAGTATTGCCAGTTTCACTCATCA

Coding sequence (CDS)

ATGGATGGGAAGAAGCTAAGTTGGACAGTAGCTGATGCTGTGGATTACAAAGGTTATCCAGCAGATAAAACTAAGACTGGTGGTTGGATTCCTGCTGCTCTTATTTTAGGTATTGAAATATGTGAAAGGCTGTCAACAATGGGAATAGCAGTGAATCTTGTGACTTATTTGGGCGGGGTAATGCATTTGCCCAGTGCAGCTTCAGCCAACATTGTTACAGATTTCATGGGAACTTGCTTTCTCCTCTGTTTGCTTGGAGGCTTTCTTGCTGATTCTTTCCTTGGTCGATATTACACCATTCTCATCTCTGCCATCATTCAAACACTGGGGACTGCTGCACTTGCAGTTTCAACAAAACTACCACAGCTTCGTCCGCCGCCATGCAATGGCACCAAAACCTGCAAGGAAGCAAATGGATTTCAAATGGGAATTTTGTATCTAGCTTTGTATCTCATTGCTTTAGGGACTGGTGGCTTAAAATCAAGCATTTCGGGTTTCGGAACTGATCAGTTTGATGATACAGATGAGAAAGAAAAGGCTAAGATGGCCTATTTCTTTAACAGATTCTTCTTCTTTATCAGCACTGGGACTTTGATGGCTGTCACAGTGTTGGTTTATATTCAAGATGAAGTGGGTCGAAGTTGGGCGTATGGGATTTGTTCTGTTTCCATGCTTGGCGCGATTTTGTTGTTCCTTTCAGGAACCAAGAGGTACAGATACAAGAAGAGCATGGGGAGCCCTGTAGTTCATATTCTACAAGTCATTGCTGCTGCAATCAAGAAGAGAAAATTGGAGCTTCCTCAGAATGTTGGTTTGCTTTATGAGGATTCTCCTGATCAGGCTTCAAGAATTGAGCACACAAAACAATTCCAATTCTTGGACAAGGCTGCAATTGTTGCAGAAGGCGATTTCGAGACGCATGGTGCTGTTTCGGCTCTAAATCCATGGAAGCTAAGCTCAGTGACGAGAGTGGAGGAGGTGAAAATGATGGCAAGATTGCTGCCAATATGGGCCACAACCATCATCTTCTGGACTACATATGCCCAAATGATCACATTCTCAGTTCAACAAGCATCCACAATGGAAAGATCAATTGGATCTTTCCAGATTCCAGCAGGCTCTCTCACTGTCTTCTTTGTAGCAGCCATATTAATAACTTTGGGTGTTTATGATCGTCTTATCATGCCTCTTTGGAAGAAATGGAAAGGAAAACAAGGTTTCACAAACATACAAAGAATGGCCATAGGCCTTGTCCTATCAATACTTGGAATGGCAGCAGCGGTACTAGCGGAGAGGAAAAGGCTGGCAGTGGCGAAAGCAGTTGGTGGCGACACAACTACCCTACCTATAAGTGTGTTCTTATTGATCCCACAATTTTTCTTGGTGGGGGCAGGTGAAGCCTTCATATACACAGGGCAACTTGATTTCTTCATAACCAAATCTCCCAAAGGGATGAAAACTATGAGTACCGGTCTCTTCCTCTCAACATTATCCCTCGGGTTTTTCATCAGTAGTTTCTTGGTTTCAGTTGTGAAGAGGGTGACAGGAAACGATGGAGAAGGTTGGCTTGCAGACAATATAAATTATGGCAGACTTGACTGCTTCTATGGACTATTGACCATATTAAGTATCATAAACTTTGTTGCTTTTCTGGTATGTGTTATATGGTACATGCCACAAAAGCGCAAAGACTTTCAAAAGAATGGCATAGTCAATGGCAACTTGGCTGAAGAGAAATGCTAA

Protein sequence

MDGKKLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKLPQLRPPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKAKMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRYKKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVAEGDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQASTMERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITKSPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLTILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC
Homology
BLAST of PI0023612.1 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 8.2e-241
Identity = 435/562 (77.40%), Postives = 483/562 (85.94%), Query Frame = 0

Query: 5   KLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMHLP 64
           K SWTVADAVDYKG PADK+KTGGWI AALILGIE+ ERLSTMGIAVNLVTYL   MHLP
Sbjct: 4   KGSWTVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLP 63

Query: 65  SAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKLPQLR 124
           S+ SANIVTDFMGT FLLCLLGGFLADSFLGR+ TI I + IQ LGT ALAV+TKLP+LR
Sbjct: 64  SSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELR 123

Query: 125 PPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKAKMAY 184
           PP C+  + C  A  FQM ILY++LYLIALGTGGLKSSISGFG+DQFDD D KEKA MA+
Sbjct: 124 PPTCHHGEACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAF 183

Query: 185 FFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRYKKSM 244
           FFNRFFFFIS GTL+AVTVLVY+QDEVGRSWAYGIC+VSM  AI++FL GTKRYRYKKS 
Sbjct: 184 FFNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQ 243

Query: 245 GSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVAEGDF 304
           GSPVV I QVIAAA +KRK+ELPQ++  LYED+P +  RIEHT QF  LDKAAIVAEGDF
Sbjct: 244 GSPVVQIFQVIAAAFRKRKMELPQSIVYLYEDNP-EGIRIEHTDQFHLLDKAAIVAEGDF 303

Query: 305 E-THGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQASTMER 364
           E T   V+  NPWKLSSVT+VEEVKMM RLLPIWATTIIFWTTYAQMITFSV+QASTM R
Sbjct: 304 EQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRR 363

Query: 365 SIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILG 424
           +IGSF+IPAGSLTVFFVAAILITL VYDR IMP WKKWKGK GF+++QR+AIGLVLS  G
Sbjct: 364 NIGSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKKWKGKPGFSSLQRIAIGLVLSTAG 423

Query: 425 MAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITKSPK 484
           MAAA L E+KRL+VAK+      TLPISVFLL+PQFFLVGAGEAFIYTGQLDFFIT+SPK
Sbjct: 424 MAAAALVEQKRLSVAKS--SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPK 483

Query: 485 GMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGE-GWLADNINYGRLDCFYGLLTIL 544
           GMKTMSTGLFL+TLSLGFF+SSFLVS+VKRVT    + GWLADNIN+GRLD FY LL IL
Sbjct: 484 GMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVIL 543

Query: 545 SIINFVAFLVCVIWYMPQKRKD 565
           S INFV +++C +W+ P K KD
Sbjct: 544 SGINFVVYIICALWFKPTKGKD 562

BLAST of PI0023612.1 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 1.5e-165
Identity = 305/563 (54.17%), Postives = 404/563 (71.76%), Query Frame = 0

Query: 12  DAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMHLPSAASANI 71
           DA D++G PAD++KTGGW  AA+IL IE  ERL+T+GI VNLVTYL G MHL +A +AN 
Sbjct: 14  DAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANT 73

Query: 72  VTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKLPQLRPPPCNGT 131
           VT+F+GT F+LCLLGGF+AD+FLGRY TI I A IQ  G + L +ST +P LRPP CN T
Sbjct: 74  VTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPT 133

Query: 132 KT--CKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKAKMAYFFNRF 191
            +  C++A+G Q+ +LYLALYL ALGTGG+K+S+SGFG+DQFD+T+ KE++KM YFFNRF
Sbjct: 134 TSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRF 193

Query: 192 FFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRYKKSMGSPVV 251
           FF I+ G+L+AVTVLVY+QD+VGR W YGIC+ +++ A+ +FL+GT RYR+KK +GSP+ 
Sbjct: 194 FFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMT 253

Query: 252 HILQVIAAAIKKRKLELPQNVGLLY--------EDSPDQASRIEHTKQFQFLDKAAIVAE 311
            +  VI AA + RKLELP +   LY        E S     ++ HT+QF+ LDKAAI   
Sbjct: 254 QVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAI--- 313

Query: 312 GDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQASTM 371
            D E     +  N W LS++T VEEVK + R+LPIWAT I+FWT +AQ+ T SV Q+ T+
Sbjct: 314 RDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETL 373

Query: 372 ERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKK-WKGKQGFTNIQRMAIGLVLS 431
           +RSIGSF+IP  S+ VF+V  +L+T  VYDR+ + L KK +    G   +QR+ +GL   
Sbjct: 374 DRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFG 433

Query: 432 ILGMAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 491
            + MA A L E KRL  A A G    TLP+  +LLIPQ+ +VG GEA IYTGQLDFF+ +
Sbjct: 434 SMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRE 493

Query: 492 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 551
            PKGMK MSTGL LSTL+LGFF SS LV++V++ TG     W+AD++N GRL  FY L+ 
Sbjct: 494 CPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGK-AHPWIADDLNKGRLYNFYWLVA 553

Query: 552 ILSIINFVAFLVCVIWYMPQKRK 564
           +L  +NF+ FLV   WY+ ++++
Sbjct: 554 VLVALNFLIFLVFSKWYVYKEKR 572

BLAST of PI0023612.1 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.9e-144
Identity = 279/548 (50.91%), Postives = 376/548 (68.61%), Query Frame = 0

Query: 12  DAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMHLPSAASANI 71
           +A DY+G P DK+KTGGW+ A LILG E+ ER+  MGI++NLVTYL G +H+ SA SA I
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 72  VTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKLPQLRPPPCNGT 131
           VT+FMGT  LL LLGGFLAD+ LGRY  + ISA +  LG   L V+T +  +RPP C+  
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 132 K----TCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKAKMAYFFN 191
           +     C EANG Q+ +LY+ALY IALG GG+KS++SGFG+DQFD +D KE+ +M +FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 192 RFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRYKKSMGSP 251
           RF+F IS G+L AV  LVY+QD VGR W YGI + +M+ A ++ L GTKRYR+KK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 252 VVHILQVIAAAIKKRKLELPQNVGLL--YEDSPDQASRIEHTKQFQFLDKAAIVAEGDFE 311
              I +V   A KKRK   P +  LL  Y++     + + HT+  + LDKAAI       
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLLNGYDN-----TTVPHTEMLKCLDKAAISKNESSP 315

Query: 312 THGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQASTMERSI 371
           +       +PW +S+VT+VEEVK++ +L+PIWAT I+FWT Y+QM TF+V+QA+ M+R +
Sbjct: 316 SSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKL 375

Query: 372 GSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKK-WKGKQGFTNIQRMAIGLVLSILGM 431
           GSF +PAGS + F +  IL+   + +R+ +PL ++  K  QG T++QR+ +GLV S+  M
Sbjct: 376 GSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAM 435

Query: 432 AAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITKSPKG 491
           A A + E  R     AV  D     IS F L+PQ+FLVGAGEAF Y GQL+FFI ++P+ 
Sbjct: 436 AVAAVIENARREA--AVNNDK---KISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPER 495

Query: 492 MKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLTILSI 551
           MK+MSTGLFLST+S+GFF+SS LVS+V RVT    + WL  N+N  RL+ FY LL +L  
Sbjct: 496 MKSMSTGLFLSTISMGFFVSSLLVSLVDRVT---DKSWLRSNLNKARLNYFYWLLVVLGA 550

Query: 552 INFVAFLV 553
           +NF+ F+V
Sbjct: 556 LNFLIFIV 550

BLAST of PI0023612.1 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 3.2e-120
Identity = 240/570 (42.11%), Postives = 367/570 (64.39%), Query Frame = 0

Query: 1   MDGKKLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           M+  K  +T    +D    PA+K KTG W     ILG E CERL+  G++ NL+ YL   
Sbjct: 1   MEDDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQ 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120
           M++ + +++  V+++ GTC+   L+G F+AD++LGRY+TI    +I   G   L +S  +
Sbjct: 61  MNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASV 120

Query: 121 PQLRPPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180
           P L  P C+G +TC    G Q  I ++ALYLIALGTGG+K  +S FG DQFDDTDEKEK 
Sbjct: 121 PGL-TPTCSG-ETCHATAG-QTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKE 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240
             + FFN F+F I+ G ++A +VLV+IQ  VG  W  G+ +V+M  A++ F +G+  YR 
Sbjct: 181 SKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRL 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQAS-----RIEHTKQFQFLDK 300
           +K  GSP+  +LQVI A+ +K K+++P++  LLYE+   ++S     ++EHTK   F DK
Sbjct: 241 QKPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDK 300

Query: 301 AAIVAEGDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSV 360
           AA+  E D    GA  + + WKL +VT+VEE+K + RLLPIWAT I+F + Y+QM T  V
Sbjct: 301 AAVETESD--NKGAAKS-SSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFV 360

Query: 361 QQASTMERSIG-SFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKG-KQGFTNIQRM 420
            Q +T+++ +G +F+IP+ SL++F   ++L    VYD+LI+P  +K+ G ++GFT +QR+
Sbjct: 361 LQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRI 420

Query: 421 AIGLVLSILGMAAAVLAERKRLAVAKAVG-GDTTTLPISVFLLIPQFFLVGAGEAFIYTG 480
            IGLV+SI  M +A + E  RL   +     +  T+P+++F  +PQ+FLVG  E F + G
Sbjct: 421 GIGLVISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIG 480

Query: 481 QLDFFITKSPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGE-GWLADNINYGR 540
           QL+FF  ++P  M+++ + L L+ ++ G ++S+FLV++V +VT + G  GW+A N+N G 
Sbjct: 481 QLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGH 540

Query: 541 LDCFYGLLTILSIINFVAFLVCVIWYMPQK 562
           LD F+ LL  LS +NF+ +L    WY  +K
Sbjct: 541 LDYFFWLLAGLSFLNFLVYLWIAKWYTYKK 564

BLAST of PI0023612.1 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 4.2e-120
Identity = 236/553 (42.68%), Postives = 354/553 (64.01%), Query Frame = 0

Query: 8   WTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMHLPSAA 67
           +T    VD    PA+K KTG W     ILG E CERL+  G+  NLV YL   ++  +A 
Sbjct: 7   YTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNAT 66

Query: 68  SANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKLPQLRPPP 127
           +AN VT++ GTC++  L+G F+AD++LGRY+TI     I   G   L +S  +P L+P  
Sbjct: 67  AANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGN 126

Query: 128 CNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKAKMAYFFN 187
           CN   TC   N  Q  + ++ALY+IALGTGG+K  +S FG DQFD+ DE EK K + FFN
Sbjct: 127 CN-ADTC-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFN 186

Query: 188 RFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRYKKSMGSP 247
            F+F I+ G L+A TVLV+IQ  VG  W +G+ +V+M+ A+  F  G++ YR ++  GSP
Sbjct: 187 WFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSP 246

Query: 248 VVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQAS-----RIEHTKQFQFLDKAAIVAEG 307
           +  I QVI AA +K  +++P++  LL+E + D+++     ++ HT   +F DKAA+ ++ 
Sbjct: 247 LTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQS 306

Query: 308 DFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQASTME 367
           D    G V   NPW+L SVT+VEE+K +  LLP+WAT I+F T Y+QM T  V Q +TM+
Sbjct: 307 DSIKDGEV---NPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMD 366

Query: 368 RSIG-SFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKW-KGKQGFTNIQRMAIGLVLS 427
           + +G +F+IP+ SL++F   ++L    VYD+ I+PL +K+ + ++GFT +QRM IGLV+S
Sbjct: 367 QHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVS 426

Query: 428 ILGMAAAVLAERKRLAVAKAVGG-DTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFIT 487
           I  M  A + E  RL   K     D   + +S+F  IPQ+ L+G  E F + GQL+FF  
Sbjct: 427 IFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYD 486

Query: 488 KSPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGE-GWLADNINYGRLDCFYGL 547
           ++P  M+++ + L L+T++LG ++S+ LV+VV ++T  +G+ GW+ DN+N G LD F+ L
Sbjct: 487 QAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYL 546

Query: 548 LTILSIINFVAFL 552
           L  LS +NF+ +L
Sbjct: 547 LATLSFLNFLVYL 554

BLAST of PI0023612.1 vs. ExPASy TrEMBL
Match: L0EJZ7 (Nitrate transporter NRT1.4B OS=Cucumis sativus OX=3659 GN=Csa_1G324370 PE=2 SV=1)

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 571/580 (98.45%), Postives = 576/580 (99.31%), Query Frame = 0

Query: 1   MDGKKLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDGKKLSWTVADAVDYKG+PADK+KTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISA+IQTLGTAALAVSTKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISALIQTLGTAALAVSTKL 120

Query: 121 PQLRPPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180
           PQLRPPPC+GTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300
           KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA
Sbjct: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300

Query: 301 EGDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360
           EGDFE HGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST
Sbjct: 301 EGDFEKHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480
           ILGMAAA LAE KRL VAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAESKRLGVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADN+NYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNVNYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 581
           ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of PI0023612.1 vs. ExPASy TrEMBL
Match: A0A5A7TZJ0 (Protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G00850 PE=3 SV=1)

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 566/580 (97.59%), Postives = 573/580 (98.79%), Query Frame = 0

Query: 1   MDGKKLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDGKKLSWTVADAVDYKG+PADK+KTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTA LAVSTKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTATLAVSTKL 120

Query: 121 PQLRPPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180
           PQLRPPPC+GTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSML AILLF+SGTKRYRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLIAILLFVSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300
           KKSMGSPVV ILQVIAAAIKKRKLELPQNVGLLYEDS D ASRIEHTKQFQFLDKAAIVA
Sbjct: 241 KKSMGSPVVQILQVIAAAIKKRKLELPQNVGLLYEDSSDHASRIEHTKQFQFLDKAAIVA 300

Query: 301 EGDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360
           EGDF+ HGAVSALNPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST
Sbjct: 301 EGDFQAHGAVSALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480
           ILGMAAA LAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFL+TLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 581
           ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of PI0023612.1 vs. ExPASy TrEMBL
Match: A0A1S3B9G1 (protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo OX=3656 GN=LOC103487457 PE=3 SV=1)

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 566/580 (97.59%), Postives = 573/580 (98.79%), Query Frame = 0

Query: 1   MDGKKLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDGKKLSWTVADAVDYKG+PADK+KTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTA LAVSTKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTATLAVSTKL 120

Query: 121 PQLRPPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180
           PQLRPPPC+GTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSML AILLF+SGTKRYRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLIAILLFVSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300
           KKSMGSPVV ILQVIAAAIKKRKLELPQNVGLLYEDS D ASRIEHTKQFQFLDKAAIVA
Sbjct: 241 KKSMGSPVVQILQVIAAAIKKRKLELPQNVGLLYEDSSDHASRIEHTKQFQFLDKAAIVA 300

Query: 301 EGDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360
           EGDF+ HGAVSALNPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST
Sbjct: 301 EGDFQAHGAVSALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480
           ILGMAAA LAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFL+TLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 581
           ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of PI0023612.1 vs. ExPASy TrEMBL
Match: A0A5D3DTZ7 (Protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold876G00110 PE=3 SV=1)

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 565/580 (97.41%), Postives = 573/580 (98.79%), Query Frame = 0

Query: 1   MDGKKLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDGKKLSWTVADAVDYKG+PADK+KTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTA LAVSTKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTATLAVSTKL 120

Query: 121 PQLRPPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180
           PQLRPPPC+GTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSML AILLF+SGTKRYRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLIAILLFVSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300
           KKS+GSPVV ILQVIAAAIKKRKLELPQNVGLLYEDS D ASRIEHTKQFQFLDKAAIVA
Sbjct: 241 KKSIGSPVVQILQVIAAAIKKRKLELPQNVGLLYEDSSDHASRIEHTKQFQFLDKAAIVA 300

Query: 301 EGDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360
           EGDF+ HGAVSALNPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST
Sbjct: 301 EGDFQAHGAVSALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480
           ILGMAAA LAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFL+TLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 581
           ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of PI0023612.1 vs. ExPASy TrEMBL
Match: A0A6J1JHI8 (protein NRT1/ PTR FAMILY 6.2-like OS=Cucurbita maxima OX=3661 GN=LOC111485151 PE=3 SV=1)

HSP 1 Score: 1068.9 bits (2763), Expect = 7.3e-309
Identity = 543/580 (93.62%), Postives = 562/580 (96.90%), Query Frame = 0

Query: 1   MDGKKLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDG KLSWTVADAVDYKG+PA+K+KTGGW+PAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDG-KLSWTVADAVDYKGFPAEKSKTGGWVPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISA IQTLGTAALAVSTKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAVSTKL 120

Query: 121 PQLRPPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180
           PQLRPPPC+G K CKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFD+TDEKEKA
Sbjct: 121 PQLRPPPCDGIKDCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDETDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240
           KMA+FFNRFF FISTGTLMAVTVLVYIQDEVGRSWAYGICSVSML AI LFLSGTKRYRY
Sbjct: 181 KMAFFFNRFFLFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAISLFLSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300
           KKS GSPVVHILQVIAAAIKKRKLELPQNVG+LYEDS +Q SRIEHTKQFQFLDKAAIV 
Sbjct: 241 KKSKGSPVVHILQVIAAAIKKRKLELPQNVGMLYEDSSEQGSRIEHTKQFQFLDKAAIVE 300

Query: 301 EGDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360
           +GDFE HGA SALNPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST
Sbjct: 301 QGDFEAHGAASALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           MERSIGSFQIPAGSLTVFFVAAILITLGVYDR+IMPLWKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRIIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480
           ILGMAAA +AERKRLAVAKAVGGDT TLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAAIAERKRLAVAKAVGGDTATLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540
           +PKGMKTMSTGLFL+TLSLGFFISSFLVSVVKRVTG DGEGWLADNINYGRLDCFYGLLT
Sbjct: 481 APKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGTDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 581
           ILS+INFVAFLVCVIWY+PQKRKD QK+GIVN N+AEEKC
Sbjct: 541 ILSLINFVAFLVCVIWYVPQKRKDQQKSGIVNSNMAEEKC 579

BLAST of PI0023612.1 vs. NCBI nr
Match: XP_011656291.1 (protein NRT1/ PTR FAMILY 6.2 [Cucumis sativus] >AGA60121.1 nitrate transporter NRT1.4B [Cucumis sativus] >KGN65321.1 hypothetical protein Csa_019823 [Cucumis sativus])

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 571/580 (98.45%), Postives = 576/580 (99.31%), Query Frame = 0

Query: 1   MDGKKLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDGKKLSWTVADAVDYKG+PADK+KTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISA+IQTLGTAALAVSTKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISALIQTLGTAALAVSTKL 120

Query: 121 PQLRPPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180
           PQLRPPPC+GTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300
           KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA
Sbjct: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300

Query: 301 EGDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360
           EGDFE HGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST
Sbjct: 301 EGDFEKHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480
           ILGMAAA LAE KRL VAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAESKRLGVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADN+NYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNVNYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 581
           ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of PI0023612.1 vs. NCBI nr
Match: XP_008444009.1 (PREDICTED: protein NRT1/ PTR FAMILY 6.2-like [Cucumis melo] >KAA0046795.1 protein NRT1/ PTR FAMILY 6.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 566/580 (97.59%), Postives = 573/580 (98.79%), Query Frame = 0

Query: 1   MDGKKLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDGKKLSWTVADAVDYKG+PADK+KTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTA LAVSTKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTATLAVSTKL 120

Query: 121 PQLRPPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180
           PQLRPPPC+GTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSML AILLF+SGTKRYRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLIAILLFVSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300
           KKSMGSPVV ILQVIAAAIKKRKLELPQNVGLLYEDS D ASRIEHTKQFQFLDKAAIVA
Sbjct: 241 KKSMGSPVVQILQVIAAAIKKRKLELPQNVGLLYEDSSDHASRIEHTKQFQFLDKAAIVA 300

Query: 301 EGDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360
           EGDF+ HGAVSALNPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST
Sbjct: 301 EGDFQAHGAVSALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480
           ILGMAAA LAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFL+TLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 581
           ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of PI0023612.1 vs. NCBI nr
Match: TYK26929.1 (protein NRT1/ PTR FAMILY 6.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 565/580 (97.41%), Postives = 573/580 (98.79%), Query Frame = 0

Query: 1   MDGKKLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDGKKLSWTVADAVDYKG+PADK+KTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTA LAVSTKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTATLAVSTKL 120

Query: 121 PQLRPPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180
           PQLRPPPC+GTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSML AILLF+SGTKRYRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLIAILLFVSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300
           KKS+GSPVV ILQVIAAAIKKRKLELPQNVGLLYEDS D ASRIEHTKQFQFLDKAAIVA
Sbjct: 241 KKSIGSPVVQILQVIAAAIKKRKLELPQNVGLLYEDSSDHASRIEHTKQFQFLDKAAIVA 300

Query: 301 EGDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360
           EGDF+ HGAVSALNPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST
Sbjct: 301 EGDFQAHGAVSALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480
           ILGMAAA LAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFL+TLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 581
           ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of PI0023612.1 vs. NCBI nr
Match: XP_038879372.1 (protein NRT1/ PTR FAMILY 6.2-like [Benincasa hispida])

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 559/580 (96.38%), Postives = 571/580 (98.45%), Query Frame = 0

Query: 1   MDGKKLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDGKKLSWTVADAVDYKG+PADK+KTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120
           MHLPS+ASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL
Sbjct: 61  MHLPSSASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120

Query: 121 PQLRPPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180
           PQLRPPPC+ TKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA
Sbjct: 121 PQLRPPPCDDTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEV RSWAYGICSVSML AILLFLSGT++YRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVDRSWAYGICSVSMLVAILLFLSGTRKYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300
           KKS+GSPVVHILQVIAAAIKKRK+ELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA
Sbjct: 241 KKSIGSPVVHILQVIAAAIKKRKMELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300

Query: 301 EGDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360
           EGDFE HGAVS LNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST
Sbjct: 301 EGDFEKHGAVSVLNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480
           ILGMAAA LAERKRLAVA+AVGGDT TLPISVFLLIPQFFLVGAGEAFIYTGQLDFFIT+
Sbjct: 421 ILGMAAAALAERKRLAVAEAVGGDTATLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITR 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFLSTLS GFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLSTLSFGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 581
           ILSIINFVAFLVCVIWYMPQKR+DFQK+ IVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKREDFQKSSIVNGNLAEEKC 580

BLAST of PI0023612.1 vs. NCBI nr
Match: KAG6589853.1 (Protein NRT1/ PTR FAMILY 6.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1071.2 bits (2769), Expect = 3.1e-309
Identity = 544/580 (93.79%), Postives = 563/580 (97.07%), Query Frame = 0

Query: 1   MDGKKLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDG KLSWTVADAVDYKG+PA+K+KTGGW+PAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDG-KLSWTVADAVDYKGFPAEKSKTGGWVPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISA IQTLGTAALAVSTKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAVSTKL 120

Query: 121 PQLRPPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180
           PQLRPPPC+G K CKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFD+TDEKEKA
Sbjct: 121 PQLRPPPCDGIKDCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDETDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240
           KMA+FFNRFF FISTGTLMAVTVLVYIQDEVGRSWAYGICSVSML AI LFLSGTKRYRY
Sbjct: 181 KMAFFFNRFFLFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAISLFLSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300
           KKS GSPVVHILQVIAAAIKKRKLELPQNVG+LYEDS +Q SRIEHTKQFQFLDKAAIV 
Sbjct: 241 KKSKGSPVVHILQVIAAAIKKRKLELPQNVGMLYEDSSEQGSRIEHTKQFQFLDKAAIVE 300

Query: 301 EGDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360
           +GDFE HGA SALNPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST
Sbjct: 301 QGDFEAHGAASALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           MERSIGSFQIPAGSLTVFFVAAILITLGVYDR+IMPLWKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRIIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480
           ILGMAAA +AERKRLAVAKAVGGDT TLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAAIAERKRLAVAKAVGGDTATLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFL+TLSLGFFISSFLVSVVKRVTG DGEGWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGTDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 581
           ILS+INFVAFLVCVIWY+PQKRKD QK+GIVNGN+AEE+C
Sbjct: 541 ILSLINFVAFLVCVIWYVPQKRKDQQKSGIVNGNMAEERC 579

BLAST of PI0023612.1 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 834.3 bits (2154), Expect = 5.8e-242
Identity = 435/562 (77.40%), Postives = 483/562 (85.94%), Query Frame = 0

Query: 5   KLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMHLP 64
           K SWTVADAVDYKG PADK+KTGGWI AALILGIE+ ERLSTMGIAVNLVTYL   MHLP
Sbjct: 4   KGSWTVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLP 63

Query: 65  SAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKLPQLR 124
           S+ SANIVTDFMGT FLLCLLGGFLADSFLGR+ TI I + IQ LGT ALAV+TKLP+LR
Sbjct: 64  SSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELR 123

Query: 125 PPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKAKMAY 184
           PP C+  + C  A  FQM ILY++LYLIALGTGGLKSSISGFG+DQFDD D KEKA MA+
Sbjct: 124 PPTCHHGEACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAF 183

Query: 185 FFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRYKKSM 244
           FFNRFFFFIS GTL+AVTVLVY+QDEVGRSWAYGIC+VSM  AI++FL GTKRYRYKKS 
Sbjct: 184 FFNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQ 243

Query: 245 GSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVAEGDF 304
           GSPVV I QVIAAA +KRK+ELPQ++  LYED+P +  RIEHT QF  LDKAAIVAEGDF
Sbjct: 244 GSPVVQIFQVIAAAFRKRKMELPQSIVYLYEDNP-EGIRIEHTDQFHLLDKAAIVAEGDF 303

Query: 305 E-THGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQASTMER 364
           E T   V+  NPWKLSSVT+VEEVKMM RLLPIWATTIIFWTTYAQMITFSV+QASTM R
Sbjct: 304 EQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRR 363

Query: 365 SIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILG 424
           +IGSF+IPAGSLTVFFVAAILITL VYDR IMP WKKWKGK GF+++QR+AIGLVLS  G
Sbjct: 364 NIGSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKKWKGKPGFSSLQRIAIGLVLSTAG 423

Query: 425 MAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITKSPK 484
           MAAA L E+KRL+VAK+      TLPISVFLL+PQFFLVGAGEAFIYTGQLDFFIT+SPK
Sbjct: 424 MAAAALVEQKRLSVAKS--SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPK 483

Query: 485 GMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGE-GWLADNINYGRLDCFYGLLTIL 544
           GMKTMSTGLFL+TLSLGFF+SSFLVS+VKRVT    + GWLADNIN+GRLD FY LL IL
Sbjct: 484 GMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVIL 543

Query: 545 SIINFVAFLVCVIWYMPQKRKD 565
           S INFV +++C +W+ P K KD
Sbjct: 544 SGINFVVYIICALWFKPTKGKD 562

BLAST of PI0023612.1 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 584.3 bits (1505), Expect = 1.1e-166
Identity = 305/563 (54.17%), Postives = 404/563 (71.76%), Query Frame = 0

Query: 12  DAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMHLPSAASANI 71
           DA D++G PAD++KTGGW  AA+IL IE  ERL+T+GI VNLVTYL G MHL +A +AN 
Sbjct: 14  DAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANT 73

Query: 72  VTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKLPQLRPPPCNGT 131
           VT+F+GT F+LCLLGGF+AD+FLGRY TI I A IQ  G + L +ST +P LRPP CN T
Sbjct: 74  VTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPT 133

Query: 132 KT--CKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKAKMAYFFNRF 191
            +  C++A+G Q+ +LYLALYL ALGTGG+K+S+SGFG+DQFD+T+ KE++KM YFFNRF
Sbjct: 134 TSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRF 193

Query: 192 FFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRYKKSMGSPVV 251
           FF I+ G+L+AVTVLVY+QD+VGR W YGIC+ +++ A+ +FL+GT RYR+KK +GSP+ 
Sbjct: 194 FFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMT 253

Query: 252 HILQVIAAAIKKRKLELPQNVGLLY--------EDSPDQASRIEHTKQFQFLDKAAIVAE 311
            +  VI AA + RKLELP +   LY        E S     ++ HT+QF+ LDKAAI   
Sbjct: 254 QVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAI--- 313

Query: 312 GDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQASTM 371
            D E     +  N W LS++T VEEVK + R+LPIWAT I+FWT +AQ+ T SV Q+ T+
Sbjct: 314 RDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETL 373

Query: 372 ERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKK-WKGKQGFTNIQRMAIGLVLS 431
           +RSIGSF+IP  S+ VF+V  +L+T  VYDR+ + L KK +    G   +QR+ +GL   
Sbjct: 374 DRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFG 433

Query: 432 ILGMAAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 491
            + MA A L E KRL  A A G    TLP+  +LLIPQ+ +VG GEA IYTGQLDFF+ +
Sbjct: 434 SMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRE 493

Query: 492 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 551
            PKGMK MSTGL LSTL+LGFF SS LV++V++ TG     W+AD++N GRL  FY L+ 
Sbjct: 494 CPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGK-AHPWIADDLNKGRLYNFYWLVA 553

Query: 552 ILSIINFVAFLVCVIWYMPQKRK 564
           +L  +NF+ FLV   WY+ ++++
Sbjct: 554 VLVALNFLIFLVFSKWYVYKEKR 572

BLAST of PI0023612.1 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 514.2 bits (1323), Expect = 1.3e-145
Identity = 279/548 (50.91%), Postives = 376/548 (68.61%), Query Frame = 0

Query: 12  DAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMHLPSAASANI 71
           +A DY+G P DK+KTGGW+ A LILG E+ ER+  MGI++NLVTYL G +H+ SA SA I
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 72  VTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKLPQLRPPPCNGT 131
           VT+FMGT  LL LLGGFLAD+ LGRY  + ISA +  LG   L V+T +  +RPP C+  
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 132 K----TCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKAKMAYFFN 191
           +     C EANG Q+ +LY+ALY IALG GG+KS++SGFG+DQFD +D KE+ +M +FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 192 RFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRYKKSMGSP 251
           RF+F IS G+L AV  LVY+QD VGR W YGI + +M+ A ++ L GTKRYR+KK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 252 VVHILQVIAAAIKKRKLELPQNVGLL--YEDSPDQASRIEHTKQFQFLDKAAIVAEGDFE 311
              I +V   A KKRK   P +  LL  Y++     + + HT+  + LDKAAI       
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLLNGYDN-----TTVPHTEMLKCLDKAAISKNESSP 315

Query: 312 THGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQASTMERSI 371
           +       +PW +S+VT+VEEVK++ +L+PIWAT I+FWT Y+QM TF+V+QA+ M+R +
Sbjct: 316 SSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKL 375

Query: 372 GSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKK-WKGKQGFTNIQRMAIGLVLSILGM 431
           GSF +PAGS + F +  IL+   + +R+ +PL ++  K  QG T++QR+ +GLV S+  M
Sbjct: 376 GSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAM 435

Query: 432 AAAVLAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITKSPKG 491
           A A + E  R     AV  D     IS F L+PQ+FLVGAGEAF Y GQL+FFI ++P+ 
Sbjct: 436 AVAAVIENARREA--AVNNDK---KISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPER 495

Query: 492 MKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLTILSI 551
           MK+MSTGLFLST+S+GFF+SS LVS+V RVT    + WL  N+N  RL+ FY LL +L  
Sbjct: 496 MKSMSTGLFLSTISMGFFVSSLLVSLVDRVT---DKSWLRSNLNKARLNYFYWLLVVLGA 550

Query: 552 INFVAFLV 553
           +NF+ F+V
Sbjct: 556 LNFLIFIV 550

BLAST of PI0023612.1 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 433.7 bits (1114), Expect = 2.3e-121
Identity = 240/570 (42.11%), Postives = 367/570 (64.39%), Query Frame = 0

Query: 1   MDGKKLSWTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           M+  K  +T    +D    PA+K KTG W     ILG E CERL+  G++ NL+ YL   
Sbjct: 1   MEDDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQ 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120
           M++ + +++  V+++ GTC+   L+G F+AD++LGRY+TI    +I   G   L +S  +
Sbjct: 61  MNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASV 120

Query: 121 PQLRPPPCNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180
           P L  P C+G +TC    G Q  I ++ALYLIALGTGG+K  +S FG DQFDDTDEKEK 
Sbjct: 121 PGL-TPTCSG-ETCHATAG-QTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKE 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240
             + FFN F+F I+ G ++A +VLV+IQ  VG  W  G+ +V+M  A++ F +G+  YR 
Sbjct: 181 SKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRL 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQAS-----RIEHTKQFQFLDK 300
           +K  GSP+  +LQVI A+ +K K+++P++  LLYE+   ++S     ++EHTK   F DK
Sbjct: 241 QKPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDK 300

Query: 301 AAIVAEGDFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSV 360
           AA+  E D    GA  + + WKL +VT+VEE+K + RLLPIWAT I+F + Y+QM T  V
Sbjct: 301 AAVETESD--NKGAAKS-SSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFV 360

Query: 361 QQASTMERSIG-SFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKG-KQGFTNIQRM 420
            Q +T+++ +G +F+IP+ SL++F   ++L    VYD+LI+P  +K+ G ++GFT +QR+
Sbjct: 361 LQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRI 420

Query: 421 AIGLVLSILGMAAAVLAERKRLAVAKAVG-GDTTTLPISVFLLIPQFFLVGAGEAFIYTG 480
            IGLV+SI  M +A + E  RL   +     +  T+P+++F  +PQ+FLVG  E F + G
Sbjct: 421 GIGLVISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIG 480

Query: 481 QLDFFITKSPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGE-GWLADNINYGR 540
           QL+FF  ++P  M+++ + L L+ ++ G ++S+FLV++V +VT + G  GW+A N+N G 
Sbjct: 481 QLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGH 540

Query: 541 LDCFYGLLTILSIINFVAFLVCVIWYMPQK 562
           LD F+ LL  LS +NF+ +L    WY  +K
Sbjct: 541 LDYFFWLLAGLSFLNFLVYLWIAKWYTYKK 564

BLAST of PI0023612.1 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 433.3 bits (1113), Expect = 3.0e-121
Identity = 236/553 (42.68%), Postives = 354/553 (64.01%), Query Frame = 0

Query: 8   WTVADAVDYKGYPADKTKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMHLPSAA 67
           +T    VD    PA+K KTG W     ILG E CERL+  G+  NLV YL   ++  +A 
Sbjct: 7   YTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNAT 66

Query: 68  SANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKLPQLRPPP 127
           +AN VT++ GTC++  L+G F+AD++LGRY+TI     I   G   L +S  +P L+P  
Sbjct: 67  AANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGN 126

Query: 128 CNGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKAKMAYFFN 187
           CN   TC   N  Q  + ++ALY+IALGTGG+K  +S FG DQFD+ DE EK K + FFN
Sbjct: 127 CN-ADTC-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFN 186

Query: 188 RFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRYKKSMGSP 247
            F+F I+ G L+A TVLV+IQ  VG  W +G+ +V+M+ A+  F  G++ YR ++  GSP
Sbjct: 187 WFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSP 246

Query: 248 VVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQAS-----RIEHTKQFQFLDKAAIVAEG 307
           +  I QVI AA +K  +++P++  LL+E + D+++     ++ HT   +F DKAA+ ++ 
Sbjct: 247 LTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQS 306

Query: 308 DFETHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQASTME 367
           D    G V   NPW+L SVT+VEE+K +  LLP+WAT I+F T Y+QM T  V Q +TM+
Sbjct: 307 DSIKDGEV---NPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMD 366

Query: 368 RSIG-SFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKW-KGKQGFTNIQRMAIGLVLS 427
           + +G +F+IP+ SL++F   ++L    VYD+ I+PL +K+ + ++GFT +QRM IGLV+S
Sbjct: 367 QHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVS 426

Query: 428 ILGMAAAVLAERKRLAVAKAVGG-DTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFIT 487
           I  M  A + E  RL   K     D   + +S+F  IPQ+ L+G  E F + GQL+FF  
Sbjct: 427 IFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYD 486

Query: 488 KSPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGE-GWLADNINYGRLDCFYGL 547
           ++P  M+++ + L L+T++LG ++S+ LV+VV ++T  +G+ GW+ DN+N G LD F+ L
Sbjct: 487 QAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYL 546

Query: 548 LTILSIINFVAFL 552
           L  LS +NF+ +L
Sbjct: 547 LATLSFLNFLVYL 554

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZY48.2e-24177.40Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1[more]
Q050851.5e-16554.17Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1[more]
Q9LVE01.9e-14450.91Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1[more]
Q9LFB83.2e-12042.11Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Q9M3904.2e-12042.68Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
L0EJZ70.0e+0098.45Nitrate transporter NRT1.4B OS=Cucumis sativus OX=3659 GN=Csa_1G324370 PE=2 SV=1[more]
A0A5A7TZJ00.0e+0097.59Protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A1S3B9G10.0e+0097.59protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo OX=3656 GN=LOC103487457 PE=3 S... [more]
A0A5D3DTZ70.0e+0097.41Protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A6J1JHI87.3e-30993.62protein NRT1/ PTR FAMILY 6.2-like OS=Cucurbita maxima OX=3661 GN=LOC111485151 PE... [more]
Match NameE-valueIdentityDescription
XP_011656291.10.0e+0098.45protein NRT1/ PTR FAMILY 6.2 [Cucumis sativus] >AGA60121.1 nitrate transporter N... [more]
XP_008444009.10.0e+0097.59PREDICTED: protein NRT1/ PTR FAMILY 6.2-like [Cucumis melo] >KAA0046795.1 protei... [more]
TYK26929.10.0e+0097.41protein NRT1/ PTR FAMILY 6.2-like [Cucumis melo var. makuwa][more]
XP_038879372.10.0e+0096.38protein NRT1/ PTR FAMILY 6.2-like [Benincasa hispida][more]
KAG6589853.13.1e-30993.79Protein NRT1/ PTR FAMILY 6.2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT2G26690.15.8e-24277.40Major facilitator superfamily protein [more]
AT1G12110.11.1e-16654.17nitrate transporter 1.1 [more]
AT3G21670.11.3e-14550.91Major facilitator superfamily protein [more]
AT5G01180.12.3e-12142.11peptide transporter 5 [more]
AT3G54140.13.0e-12142.68peptide transporter 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 3..565
e-value: 1.0E-186
score: 623.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 23..563
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 97..522
e-value: 1.1E-105
score: 353.7
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 9..567
NoneNo IPR availablePANTHERPTHR11654:SF85PROTEIN NRT1/ PTR FAMILY 6.2coord: 9..567
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 86..110

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
PI0023612PI0023612gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0023612.1-five_prime_utrPI0023612.1-five_prime_utr-chr02:18733068..18733194five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0023612.1-exonPI0023612.1-exon-chr02:18733068..18733197exon
PI0023612.1-exonPI0023612.1-exon-chr02:18733321..18733426exon
PI0023612.1-exonPI0023612.1-exon-chr02:18735137..18735354exon
PI0023612.1-exonPI0023612.1-exon-chr02:18735446..18735990exon
PI0023612.1-exonPI0023612.1-exon-chr02:18736136..18736479exon
PI0023612.1-exonPI0023612.1-exon-chr02:18736567..18737242exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0023612.1-cdsPI0023612.1-cds-chr02:18733195..18733197CDS
PI0023612.1-cdsPI0023612.1-cds-chr02:18733321..18733426CDS
PI0023612.1-cdsPI0023612.1-cds-chr02:18735137..18735354CDS
PI0023612.1-cdsPI0023612.1-cds-chr02:18735446..18735990CDS
PI0023612.1-cdsPI0023612.1-cds-chr02:18736136..18736479CDS
PI0023612.1-cdsPI0023612.1-cds-chr02:18736567..18737093CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0023612.1-three_prime_utrPI0023612.1-three_prime_utr-chr02:18737094..18737242three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
PI0023612.1PI0023612.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
biological_process GO:0006817 phosphate ion transport
biological_process GO:0050896 response to stimulus
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity