PI0017450.1 (mRNA) Melon (PI 482460) v1

Overview


Name	PI0017450.1
Type	mRNA
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	EF hand calcium-binding protein family
Location	chr03: 25421069 .. 25421995 (+)
Sequence length	927
RNA-Seq Expression	PI0017450.1
Synteny	PI0017450.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTCTTCTTCTTCTTCTTCTTCGCAGAATTAGGGATTTTTCCACGTTTTCTAGACTCTTTAATTCATTAATTAATACCACCAATATTTTTCCCGCACAATTCTTTATTCAACAATCGACTTCATTGAATGGGTGTAACAACATAAAAAGTAATGGTCGACCATCTACTCCACCAGTTGACGGCAACTACCATTCTCATTTACCAATTCCGACGGTCAAACCACGGTACCGCTACGCGCATTCCCTTTCATATAATTTCACACCAGATTTTGCCCTAATCACCCAATTCTTCAACTTCATTTCTCTCTTACTTACCCTTCACTCGAATTCAAAATCATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAAGTCGACGGCGATGGCGATGGGAAACTCTCTCCGCCAGAACTGCAGCGGTGTATAGCGGGAGTTGGGGGCAGTTTGACGATCGAGGAGGCGGAGACGGTGGTTGAGAAATTGGATTCGGACGGTGATGGGTTGGTGGGATGGGATGAATTTGTTGAATTTGTGGAAGGAGTTGGAGAGGATGAAAAAGTGAATGATTTGAAAGAAGCGTTTAGGATGTATGAAATGGATGGTTGTGGATTTATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAAGCTTGGGGAATCAAGGAGTATTGAGGATTGCAAGAAAATGATTGCTAAATTTGATCTTGATTCTGATGGTGTTCTTAATTTTGATGAGTTTAAGTTCATGATGTCTTGAACTTTCTTCCATTGTTTTTTTGTAGCTTCTCTCAACTTTTTTTTTGTAATGTAATATGATTGCTTCTTTGATTTTGTTTTTCTTTAATGGGTTGCTTTCTCTTTCTTTTTCTCCAAACTTGAAGCCAG

mRNA sequence

TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTCTTCTTCTTCTTCTTCTTCGCAGAATTAGGGATTTTTCCACGTTTTCTAGACTCTTTAATTCATTAATTAATACCACCAATATTTTTCCCGCACAATTCTTTATTCAACAATCGACTTCATTGAATGGGTGTAACAACATAAAAAGTAATGGTCGACCATCTACTCCACCAGTTGACGGCAACTACCATTCTCATTTACCAATTCCGACGGTCAAACCACGGTACCGCTACGCGCATTCCCTTTCATATAATTTCACACCAGATTTTGCCCTAATCACCCAATTCTTCAACTTCATTTCTCTCTTACTTACCCTTCACTCGAATTCAAAATCATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAAGTCGACGGCGATGGCGATGGGAAACTCTCTCCGCCAGAACTGCAGCGGTGTATAGCGGGAGTTGGGGGCAGTTTGACGATCGAGGAGGCGGAGACGGTGGTTGAGAAATTGGATTCGGACGGTGATGGGTTGGTGGGATGGGATGAATTTGTTGAATTTGTGGAAGGAGTTGGAGAGGATGAAAAAGTGAATGATTTGAAAGAAGCGTTTAGGATGTATGAAATGGATGGTTGTGGATTTATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAAGCTTGGGGAATCAAGGAGTATTGAGGATTGCAAGAAAATGATTGCTAAATTTGATCTTGATTCTGATGGTGTTCTTAATTTTGATGAGTTTAAGTTCATGATGTCTTGAACTTTCTTCCATTGTTTTTTTGTAGCTTCTCTCAACTTTTTTTTTGTAATGTAATATGATTGCTTCTTTGATTTTGTTTTTCTTTAATGGGTTGCTTTCTCTTTCTTTTTCTCCAAACTTGAAGCCAG

Coding sequence (CDS)

ATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAAGTCGACGGCGATGGCGATGGGAAACTCTCTCCGCCAGAACTGCAGCGGTGTATAGCGGGAGTTGGGGGCAGTTTGACGATCGAGGAGGCGGAGACGGTGGTTGAGAAATTGGATTCGGACGGTGATGGGTTGGTGGGATGGGATGAATTTGTTGAATTTGTGGAAGGAGTTGGAGAGGATGAAAAAGTGAATGATTTGAAAGAAGCGTTTAGGATGTATGAAATGGATGGTTGTGGATTTATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAAGCTTGGGGAATCAAGGAGTATTGAGGATTGCAAGAAAATGATTGCTAAATTTGATCTTGATTCTGATGGTGTTCTTAATTTTGATGAGTTTAAGTTCATGATGTCTTGA

Protein sequence

MDKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIAKFDLDSDGVLNFDEFKFMMS

Homology

[Close]

BLAST of PI0017450.1 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.4e-29
Identity = 70/139 (50.36%), Postives = 94/139 (67.63%), Query Frame = 0


Query: 5   ALYEKVFKQVDGDGDGKLSPPELQRCI-AGVGGSLTIEEAETVVEKLDSDGDGLVGWDEF 64
           A + +VF   D D DGK+S  EL+ C+ A +G  ++ EEAE +V   D+D DGL+  +EF
Sbjct: 6   AEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEF 65

Query: 65  VEF---VEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 124
            +    +E   E+E+   L EAFRMYEM+G G IT  SLKRMLSKLG  + IE+C+ MI 
Sbjct: 66  TKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMIC 125

Query: 125 KFDLDSDGVLNFDEFKFMM 140
           +FDLD DGV++F+EFK MM
Sbjct: 126 RFDLDGDGVISFEEFKIMM 144

[Close]

BLAST of PI0017450.1 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.6e-28
Identity = 66/138 (47.83%), Postives = 93/138 (67.39%), Query Frame = 0


Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGWD 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ ++
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIAK 121
           EF + ++   E+EK  +LK AFR+Y  +G   IT +SLK ML KLGESR+ +DC+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLDSDGVLNFDEFKFMM 140
           FDL++DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]

BLAST of PI0017450.1 vs. ExPASy Swiss-Prot
Match: Q9FIH9 (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 3.5e-28
Identity = 65/132 (49.24%), Postives = 92/132 (69.70%), Query Frame = 0


Query: 10  VFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVEFVEG 69
           VF  +D + DGK+S  ELQ C++ +GG+L+  E E VV+  D DGDG + ++EF++ +EG
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  V--GEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIAKFDLDSD 129
               ++E+  +LKEAF MY M+G  FIT  SL+R LS+LGES +++ CK MI  FD + D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]

BLAST of PI0017450.1 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 8.6e-27
Identity = 64/138 (46.38%), Postives = 92/138 (66.67%), Query Frame = 0


Query: 8   EKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVE-F 67
           +KVF++ D +GDGK+S  EL+  I  +  + + EE  T++++ D DG+G +  DEFV  F
Sbjct: 19  KKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALF 78

Query: 68  VEGVG----EDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIAKF 127
             G+G        V+DLKEAF +Y++DG G I+ K L  ++  LGE  S++DCKKMI+K 
Sbjct: 79  QIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISKV 138

Query: 128 DLDSDGVLNFDEFKFMMS 141
           D+D DG +NFDEFK MMS
Sbjct: 139 DIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of PI0017450.1 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.5e-26
Identity = 67/143 (46.85%), Postives = 92/143 (64.34%), Query Frame = 0


Query: 7   YEKVFKQVDGDGDGKLSPPELQRCI-AGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVE 66
           + +VF   D DGDGK+S  EL+ C+ A +G  +  EE + ++   D+DGDGL+  +EFV 
Sbjct: 7   FRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFVR 66

Query: 67  FV---EGVGEDEKVND------LKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCK 126
            V   E  G++E+ +D      L+EAF MYEM+G G IT  SLK MLSKLG    + +C+
Sbjct: 67  LVTEMEADGDEEEDDDDETCRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDVAECQ 126

Query: 127 KMIAKFDLDSDGVLNFDEFKFMM 140
            MI +FD++ DGVL FDEFK MM
Sbjct: 127 AMICRFDMNGDGVLTFDEFKTMM 149

[Close]

BLAST of PI0017450.1 vs. ExPASy TrEMBL
Match: A0A0A0LMM5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G406780 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 2.6e-71
Identity = 137/140 (97.86%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQRCI GVGGSLTIEEAETVVEKLDSDGDGLVGW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60

Query: 61  DEFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120
           DEFVEFVEGVGE+EKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSI+DCKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120

Query: 121 KFDLDSDGVLNFDEFKFMMS 141
           KFDLDSDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

[Close]

BLAST of PI0017450.1 vs. ExPASy TrEMBL
Match: A0A5A7UNE9 (Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00360 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 4.5e-71
Identity = 137/140 (97.86%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQRCI GVGGSLTIEEAETVVEKLDS+GDGLVGW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSNGDGLVGW 60

Query: 61  DEFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120
           DEFVEFVEGVGE+EKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120

Query: 121 KFDLDSDGVLNFDEFKFMMS 141
           KFDLDSDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

[Close]

BLAST of PI0017450.1 vs. ExPASy TrEMBL
Match: A0A1S3B3Z8 (putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103485494 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 4.5e-71
Identity = 137/140 (97.86%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQRCI GVGGSLTIEEAETVVEKLDS+GDGLVGW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSNGDGLVGW 60

Query: 61  DEFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120
           DEFVEFVEGVGE+EKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120

Query: 121 KFDLDSDGVLNFDEFKFMMS 141
           KFDLDSDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

[Close]

BLAST of PI0017450.1 vs. ExPASy TrEMBL
Match: A0A6J1FET5 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111444836 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.0e-63
Identity = 123/140 (87.86%), Postives = 133/140 (95.00%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRC+AGVGGSLT+EEAE VVE LD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 61

Query: 61  DEFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120
            EFV FVEGVGE+EKV+DLKEAFRMYEMDGCGFITTKSLKRMLS+LGESRSIE+C KMIA
Sbjct: 62  GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 121

Query: 121 KFDLDSDGVLNFDEFKFMMS 141
           KFDL+SDGVLNFDEF FMMS
Sbjct: 122 KFDLNSDGVLNFDEFMFMMS 141

[Close]

BLAST of PI0017450.1 vs. ExPASy TrEMBL
Match: A0A6J1JZS3 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111488916 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.7e-63
Identity = 123/140 (87.86%), Postives = 133/140 (95.00%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRC+AGVGGSLT+EEAE  VE LD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMAVENLDTDGDGLMGW 61

Query: 61  DEFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120
            EFV FVE VGE+EKV+DLKEAFRMYEMDGCGFITTKSLKRMLS+LGESRSIE+CKKMIA
Sbjct: 62  GEFVAFVEEVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 121

Query: 121 KFDLDSDGVLNFDEFKFMMS 141
           KFDL+SDGVLNFDEFKFMMS
Sbjct: 122 KFDLNSDGVLNFDEFKFMMS 141

[Close]

BLAST of PI0017450.1 vs. NCBI nr
Match: XP_004138612.1 (calcium-binding protein CML38 [Cucumis sativus] >KGN63180.1 hypothetical protein Csa_021832 [Cucumis sativus])

HSP 1 Score: 277.7 bits (709), Expect = 5.5e-71
Identity = 137/140 (97.86%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQRCI GVGGSLTIEEAETVVEKLDSDGDGLVGW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60

Query: 61  DEFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120
           DEFVEFVEGVGE+EKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSI+DCKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120

Query: 121 KFDLDSDGVLNFDEFKFMMS 141
           KFDLDSDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

[Close]

BLAST of PI0017450.1 vs. NCBI nr
Match: XP_008441354.1 (PREDICTED: putative calcium-binding protein CML19 [Cucumis melo] >KAA0056590.1 putative calcium-binding protein CML19 [Cucumis melo var. makuwa] >TYK24272.1 putative calcium-binding protein CML19 [Cucumis melo var. makuwa])

HSP 1 Score: 276.9 bits (707), Expect = 9.3e-71
Identity = 137/140 (97.86%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQRCI GVGGSLTIEEAETVVEKLDS+GDGLVGW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSNGDGLVGW 60

Query: 61  DEFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120
           DEFVEFVEGVGE+EKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120

Query: 121 KFDLDSDGVLNFDEFKFMMS 141
           KFDLDSDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

[Close]

BLAST of PI0017450.1 vs. NCBI nr
Match: XP_038886217.1 (putative calcium-binding protein CML19 [Benincasa hispida])

HSP 1 Score: 271.2 bits (692), Expect = 5.1e-69
Identity = 133/140 (95.00%), Postives = 138/140 (98.57%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQRC+AGVGG LTIEEAETVVE LDSDGDGL+GW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAGVGGVLTIEEAETVVENLDSDGDGLLGW 60

Query: 61  DEFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120
           DEFVEFVEGVGE+EKV+DLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSI+DCKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120

Query: 121 KFDLDSDGVLNFDEFKFMMS 141
           KFDLDSDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

[Close]

BLAST of PI0017450.1 vs. NCBI nr
Match: XP_022938684.1 (putative calcium-binding protein CML19 [Cucurbita moschata] >XP_023550776.1 putative calcium-binding protein CML19 isoform X2 [Cucurbita pepo subsp. pepo] >KAG7015957.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 251.5 bits (641), Expect = 4.2e-63
Identity = 123/140 (87.86%), Postives = 133/140 (95.00%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRC+AGVGGSLT+EEAE VVE LD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 61

Query: 61  DEFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120
            EFV FVEGVGE+EKV+DLKEAFRMYEMDGCGFITTKSLKRMLS+LGESRSIE+C KMIA
Sbjct: 62  GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 121

Query: 121 KFDLDSDGVLNFDEFKFMMS 141
           KFDL+SDGVLNFDEF FMMS
Sbjct: 122 KFDLNSDGVLNFDEFMFMMS 141

[Close]

BLAST of PI0017450.1 vs. NCBI nr
Match: KAG6578374.1 (Protoporphyrinogen oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 251.5 bits (641), Expect = 4.2e-63
Identity = 123/140 (87.86%), Postives = 133/140 (95.00%), Query Frame = 0


Query: 1    MDKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
            MDK+ALYEKVFKQVDGDGDGKLSP ELQRC+AGVGGSLT+EEAE VVE LD+DGDGL+GW
Sbjct: 1309 MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 1368

Query: 61   DEFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120
             EFV FVEGVGE+EKV+DLKEAFRMYEMDGCGFITTKSLKRMLS+LGESRSIE+C KMIA
Sbjct: 1369 GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 1428

Query: 121  KFDLDSDGVLNFDEFKFMMS 141
            KFDL+SDGVLNFDEF FMMS
Sbjct: 1429 KFDLNSDGVLNFDEFMFMMS 1448

[Close]

BLAST of PI0017450.1 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 127.1 bits (318), Expect = 1.1e-29
Identity = 66/138 (47.83%), Postives = 93/138 (67.39%), Query Frame = 0


Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGWD 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ ++
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIAK 121
           EF + ++   E+EK  +LK AFR+Y  +G   IT +SLK ML KLGESR+ +DC+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLDSDGVLNFDEFKFMM 140
           FDL++DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]

BLAST of PI0017450.1 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 127.1 bits (318), Expect = 1.1e-29
Identity = 66/138 (47.83%), Postives = 93/138 (67.39%), Query Frame = 0


Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGWD 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ ++
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVEFVEGVGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIAK 121
           EF + ++   E+EK  +LK AFR+Y  +G   IT +SLK ML KLGESR+ +DC+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLDSDGVLNFDEFKFMM 140
           FDL++DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]

BLAST of PI0017450.1 vs. TAIR 10
Match: AT5G42380.1 (calmodulin like 37 )

HSP 1 Score: 125.9 bits (315), Expect = 2.5e-29
Identity = 65/132 (49.24%), Postives = 92/132 (69.70%), Query Frame = 0


Query: 10  VFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVEFVEG 69
           VF  +D + DGK+S  ELQ C++ +GG+L+  E E VV+  D DGDG + ++EF++ +EG
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  V--GEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIAKFDLDSD 129
               ++E+  +LKEAF MY M+G  FIT  SL+R LS+LGES +++ CK MI  FD + D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]

BLAST of PI0017450.1 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 121.3 bits (303), Expect = 6.1e-28
Identity = 64/138 (46.38%), Postives = 92/138 (66.67%), Query Frame = 0


Query: 8   EKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVE-F 67
           +KVF++ D +GDGK+S  EL+  I  +  + + EE  T++++ D DG+G +  DEFV  F
Sbjct: 19  KKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALF 78

Query: 68  VEGVG----EDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIAKF 127
             G+G        V+DLKEAF +Y++DG G I+ K L  ++  LGE  S++DCKKMI+K 
Sbjct: 79  QIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISKV 138

Query: 128 DLDSDGVLNFDEFKFMMS 141
           D+D DG +NFDEFK MMS
Sbjct: 139 DIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of PI0017450.1 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 117.1 bits (292), Expect = 1.1e-26
Identity = 65/135 (48.15%), Postives = 87/135 (64.44%), Query Frame = 0


Query: 8   EKVFKQVDGDGDGKLSPPELQRCIAGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVEFV 67
           E VF  +D + DG++S  EL++    +G  ++ EEAE  V+  D DGDG++  +EF   +
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 68  EG---VGEDEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIAKFDL 127
           +G     E+EK   + EAFRMY  DG   IT  SLK ML KLGESR+ +DCK MI  FDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 128 DSDGVLNFDEFKFMM 140
           ++DGVL+FDEF  MM
Sbjct: 144 NADGVLSFDEFALMM 158

The following BLAST results are available for this feature:

BLAST of PI0017450.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RYJ8	1.4e-29	50.36	Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SRE6	1.6e-28	47.83	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q9FIH9	3.5e-28	49.24	Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1	[more]
P25070	8.6e-27	46.38	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q8RYJ9	2.5e-26	46.85	Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of PI0017450.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LMM5	2.6e-71	97.86	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G406780 PE=4 SV=1	[more]
A0A5A7UNE9	4.5e-71	97.86	Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3B3Z8	4.5e-71	97.86	putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103485494 P...	[more]
A0A6J1FET5	2.0e-63	87.86	putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1JZS3	2.7e-63	87.86	putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114889...	[more]

BLAST of PI0017450.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138612.1	5.5e-71	97.86	calcium-binding protein CML38 [Cucumis sativus] >KGN63180.1 hypothetical protein...	[more]
XP_008441354.1	9.3e-71	97.86	PREDICTED: putative calcium-binding protein CML19 [Cucumis melo] >KAA0056590.1 p...	[more]
XP_038886217.1	5.1e-69	95.00	putative calcium-binding protein CML19 [Benincasa hispida]	[more]
XP_022938684.1	4.2e-63	87.86	putative calcium-binding protein CML19 [Cucurbita moschata] >XP_023550776.1 puta...	[more]
KAG6578374.1	4.2e-63	87.86	Protoporphyrinogen oxidase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of PI0017450.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76650.1	1.1e-29	47.83	calmodulin-like 38	[more]
AT1G76650.3	1.1e-29	47.83	calmodulin-like 38	[more]
AT5G42380.1	2.5e-29	49.24	calmodulin like 37	[more]
AT5G37770.1	6.1e-28	46.38	EF hand calcium-binding protein family	[more]
AT1G76640.1	1.1e-26	48.15	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 114..140 e-value: 1.1 score: 17.5 coord: 42..70 e-value: 11.0 score: 9.4 coord: 6..34 e-value: 0.056 score: 22.6 coord: 78..106 e-value: 0.19 score: 20.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 6..67 e-value: 3.9E-14 score: 52.8 coord: 78..139 e-value: 2.0E-10 score: 40.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 38..73 score: 13.007053
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 2..37 score: 12.923368
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 110..140 score: 14.513405
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 74..109 score: 11.361225
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 78..140 e-value: 1.48001E-14 score: 61.7949
None	No IPR available	GENE3D	1.10.238.10		coord: 1..71 e-value: 2.1E-15 score: 59.2
None	No IPR available	GENE3D	1.10.238.10		coord: 72..140 e-value: 5.9E-19 score: 70.1
None	No IPR available	PANTHER	PTHR10891:SF858	EF HAND CALCIUM-BINDING FAMILY PROTEIN	coord: 2..140
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 2..140
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 51..63
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 15..27
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 123..135
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 3..139

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
PI0017450	PI0017450	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0017450.1-five_prime_utr	PI0017450.1-five_prime_utr-chr03:25421069..25421444	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0017450.1-exon	PI0017450.1-exon-chr03:25421069..25421995	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0017450.1-cds	PI0017450.1-cds-chr03:25421445..25421867	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0017450.1-three_prime_utr	PI0017450.1-three_prime_utr-chr03:25421868..25421995	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
PI0017450.1	PI0017450.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding