PI0004274.1 (mRNA) Melon (PI 482460) v1

Overview


Name	PI0004274.1
Type	mRNA
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Integrase catalytic domain-containing protein
Location	chr01: 20299000 .. 20299266 (-)
Sequence length	267
RNA-Seq Expression	PI0004274.1
Synteny	PI0004274.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTGACGCAAGTGATATGGTGGTAGAGGCTATGCTAGGTCAAAAGAAAGATAAAGTGATCCACCCTATATACTACGCGAGCAAGACTCTCACTGATGTTCAAAAGAATTATACAACCACAAAGAAGGAATTGCTTGCAGTAGTATTCACGATTGAAAAGTTTAGGAGTTATATTGTTGGCTCCAAAGTTACGGTACATTCTGATCACTCTGTGATCAGATTCCTCATGGCGAAAAAGGACACCAAGCCGTGGTTAATTAGATGA

mRNA sequence

ATGTGTGACGCAAGTGATATGGTGGTAGAGGCTATGCTAGGTCAAAAGAAAGATAAAGTGATCCACCCTATATACTACGCGAGCAAGACTCTCACTGATGTTCAAAAGAATTATACAACCACAAAGAAGGAATTGCTTGCAGTAGTATTCACGATTGAAAAGTTTAGGAGTTATATTGTTGGCTCCAAAGTTACGGTACATTCTGATCACTCTGTGATCAGATTCCTCATGGCGAAAAAGGACACCAAGCCGTGGTTAATTAGATGA

Coding sequence (CDS)

ATGTGTGACGCAAGTGATATGGTGGTAGAGGCTATGCTAGGTCAAAAGAAAGATAAAGTGATCCACCCTATATACTACGCGAGCAAGACTCTCACTGATGTTCAAAAGAATTATACAACCACAAAGAAGGAATTGCTTGCAGTAGTATTCACGATTGAAAAGTTTAGGAGTTATATTGTTGGCTCCAAAGTTACGGTACATTCTGATCACTCTGTGATCAGATTCCTCATGGCGAAAAAGGACACCAAGCCGTGGTTAATTAGATGA

Protein sequence

MCDASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIVGSKVTVHSDHSVIRFLMAKKDTKPWLIR

Homology

[Close]

BLAST of PI0004274.1 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.7e-09
Identity = 34/86 (39.53%), Postives = 51/86 (59.30%), Query Frame = 0


Query: 3   DASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIVGS 62
           DASD+ + A+L Q      HP+ Y S+TL + + NY+T +KELLA+V+  + FR Y++G 
Sbjct: 514 DASDVALGAVLSQDG----HPLSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYLLGR 573

Query: 63  KVTVHSDHSVIRFLMAKKDTKPWLIR 89
              + SDH  + +L   KD    L R
Sbjct: 574 HFEISSDHQPLSWLYRMKDPNSKLTR 595

[Close]

BLAST of PI0004274.1 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.9e-08
Identity = 31/79 (39.24%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 3   DASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIVGS 62
           DAS++ + A+L Q      HPI + S+TL D + NY+  +KELLA+V+  + FR Y++G 
Sbjct: 513 DASNLALGAVLSQNG----HPISFISRTLNDHELNYSAIEKELLAIVWATKTFRHYLLGR 572

Query: 63  KVTVHSDHSVIRFLMAKKD 82
           +  + SDH  +R+L   K+
Sbjct: 573 QFLIASDHQPLRWLHNLKE 587

[Close]

BLAST of PI0004274.1 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.8e-07
Identity = 25/80 (31.25%), Postives = 50/80 (62.50%), Query Frame = 0


Query: 3   DASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIVGS 62
           DAS+  + A+L Q       PI Y S++L   ++NY T +KE+LA++++++  R+Y+ G+
Sbjct: 440 DASNWAIGAVLSQDDQGRDRPIAYISRSLNKTEENYATIEKEMLAIIWSLDNLRAYLYGA 499

Query: 63  -KVTVHSDHSVIRFLMAKKD 82
             + V++DH  + F +  ++
Sbjct: 500 GTIKVYTDHQPLTFALGNRN 519

[Close]

BLAST of PI0004274.1 vs. ExPASy Swiss-Prot
Match: P10394 (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 1.2e-05
Identity = 30/86 (34.88%), Postives = 45/86 (52.33%), Query Frame = 0


Query: 3   DASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIVGS 62
           DAS     A+L Q  +    P+ YAS+  T  + N +TT++EL A+ + I  FR YI G 
Sbjct: 621 DASKQACGAVLTQNHNGHQLPVAYASRAFTKGESNKSTTEQELAAIHWAIIHFRPYIYGK 680

Query: 63  KVTVHSDHSVIRFLMAKKDTKPWLIR 89
             TV +DH  + +L +  +    L R
Sbjct: 681 HFTVKTDHRPLTYLFSMVNPSSKLTR 706

[Close]

BLAST of PI0004274.1 vs. ExPASy Swiss-Prot
Match: P10401 (Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.5e-05
Identity = 27/81 (33.33%), Postives = 50/81 (61.73%), Query Frame = 0


Query: 3   DASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIVGS 62
           DAS   + A+L Q+      PI   S+TL   ++NY T ++ELLA+V+ + K ++++ GS
Sbjct: 500 DASASGIGAVLSQEG----RPITMISRTLKQPEQNYATNERELLAIVWALGKLQNFLYGS 559

Query: 63  K-VTVHSDHSVIRFLMAKKDT 83
           + + + +DH  + F +A ++T
Sbjct: 560 REINIFTDHQPLTFAVADRNT 576

[Close]

BLAST of PI0004274.1 vs. ExPASy TrEMBL
Match: A0A5D3DS34 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G001700 PE=4 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.7e-29
Identity = 67/88 (76.14%), Postives = 75/88 (85.23%), Query Frame = 0


Query: 1   MCDASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIV 60
           MCDASD+VV  M GQKKDKV  PIYYA KTL + ++NYTT +KELLA+VF IEKFRSYIV
Sbjct: 78  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 137

Query: 61  GSKVTVHSDHSVIRFLMAKKDTKPWLIR 89
           GSKVT+HSDHS IR+LMAKKD K WLIR
Sbjct: 138 GSKVTIHSDHSAIRYLMAKKDAKSWLIR 165

[Close]

BLAST of PI0004274.1 vs. ExPASy TrEMBL
Match: A0A5A7V586 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold154G002210 PE=4 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.7e-29
Identity = 67/88 (76.14%), Postives = 75/88 (85.23%), Query Frame = 0


Query: 1   MCDASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIV 60
           MCDASD+VV  M GQKKDKV  PIYYA KTL + ++NYTT +KELLA+VF IEKFRSYIV
Sbjct: 52  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 111

Query: 61  GSKVTVHSDHSVIRFLMAKKDTKPWLIR 89
           GSKVT+HSDHS IR+LMAKKD K WLIR
Sbjct: 112 GSKVTIHSDHSAIRYLMAKKDAKSWLIR 139

[Close]

BLAST of PI0004274.1 vs. ExPASy TrEMBL
Match: A0A5A7V8R9 (RT_RNaseH domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G003510 PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 3.3e-27
Identity = 64/87 (73.56%), Postives = 74/87 (85.06%), Query Frame = 0


Query: 1   MCDASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIV 60
           MCDASD+V+ AMLGQK DKVI+PIY  SKTL + Q+NYTTT+KELLAVVF IEK+ SYIV
Sbjct: 30  MCDASDVVIGAMLGQKNDKVIYPIYNMSKTLNEAQENYTTTEKELLAVVFAIEKYVSYIV 89

Query: 61  GSKVTVHSDHSVIRFLMAKKDTKPWLI 88
           GSKVT+HSDH  IR+LM KKD +P LI
Sbjct: 90  GSKVTIHSDHFAIRYLMVKKDAQPQLI 116

[Close]

BLAST of PI0004274.1 vs. ExPASy TrEMBL
Match: A0A2G9HH15 (Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_10756 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 5.6e-27
Identity = 60/88 (68.18%), Postives = 73/88 (82.95%), Query Frame = 0


Query: 1    MCDASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIV 60
            MCDASD  + A+LGQ+KDK+   IYYASKTL D Q NYTTT+KELLAVVF  +KFRSY+V
Sbjct: 1195 MCDASDFAIGAVLGQRKDKIFRSIYYASKTLNDAQLNYTTTEKELLAVVFAFDKFRSYLV 1254

Query: 61   GSKVTVHSDHSVIRFLMAKKDTKPWLIR 89
            G+KV V++DH+ IR+L+ KKD KPWLIR
Sbjct: 1255 GTKVIVYTDHAAIRYLIEKKDAKPWLIR 1282

[Close]

BLAST of PI0004274.1 vs. ExPASy TrEMBL
Match: A0A6J1DHH9 (LOW QUALITY PROTEIN: uncharacterized protein LOC111020110 OS=Momordica charantia OX=3673 GN=LOC111020110 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 9.5e-27
Identity = 59/88 (67.05%), Postives = 74/88 (84.09%), Query Frame = 0


Query: 1   MCDASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIV 60
           MCDASD  + AMLGQ+K+K++HP+YYASKTLT  Q NYTTT+KELLAVVF  +KFRSY++
Sbjct: 352 MCDASDYAIGAMLGQRKNKILHPVYYASKTLTGAQLNYTTTEKELLAVVFAFDKFRSYLI 411

Query: 61  GSKVTVHSDHSVIRFLMAKKDTKPWLIR 89
           G+KV V +DHS +++L AKKD KP LIR
Sbjct: 412 GTKVIVFTDHSALKYLFAKKDAKPCLIR 439

[Close]

BLAST of PI0004274.1 vs. NCBI nr
Match: KAA0062414.1 (Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa])

HSP 1 Score: 137.5 bits (345), Expect = 5.5e-29
Identity = 67/88 (76.14%), Postives = 75/88 (85.23%), Query Frame = 0


Query: 1   MCDASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIV 60
           MCDASD+VV  M GQKKDKV  PIYYA KTL + ++NYTT +KELLA+VF IEKFRSYIV
Sbjct: 52  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 111

Query: 61  GSKVTVHSDHSVIRFLMAKKDTKPWLIR 89
           GSKVT+HSDHS IR+LMAKKD K WLIR
Sbjct: 112 GSKVTIHSDHSAIRYLMAKKDAKSWLIR 139

[Close]

BLAST of PI0004274.1 vs. NCBI nr
Match: TYK26567.1 (Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa])

HSP 1 Score: 137.5 bits (345), Expect = 5.5e-29
Identity = 67/88 (76.14%), Postives = 75/88 (85.23%), Query Frame = 0


Query: 1   MCDASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIV 60
           MCDASD+VV  M GQKKDKV  PIYYA KTL + ++NYTT +KELLA+VF IEKFRSYIV
Sbjct: 78  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 137

Query: 61  GSKVTVHSDHSVIRFLMAKKDTKPWLIR 89
           GSKVT+HSDHS IR+LMAKKD K WLIR
Sbjct: 138 GSKVTIHSDHSAIRYLMAKKDAKSWLIR 165

[Close]

BLAST of PI0004274.1 vs. NCBI nr
Match: XP_017970395.1 (PREDICTED: uncharacterized protein LOC108660654 [Theobroma cacao])

HSP 1 Score: 131.3 bits (329), Expect = 4.0e-27
Identity = 63/88 (71.59%), Postives = 75/88 (85.23%), Query Frame = 0


Query: 1    MCDASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIV 60
            MCDASD  V A+LGQ+KDK+ H IYYASKTL + QKNYTTT+KELLAVVFT +KF SY+V
Sbjct: 924  MCDASDYAVGAVLGQRKDKIFHSIYYASKTLNEAQKNYTTTEKELLAVVFTFDKFHSYLV 983

Query: 61   GSKVTVHSDHSVIRFLMAKKDTKPWLIR 89
            G+KV V+ DHS I++L+AKKDTKP LIR
Sbjct: 984  GTKVIVYIDHSAIKYLIAKKDTKPRLIR 1011

[Close]

BLAST of PI0004274.1 vs. NCBI nr
Match: WP_217833163.1 (Ty3/Gypsy family RNase HI domain-containing protein, partial [Synechococcus sp. PCC 7002])

HSP 1 Score: 131.0 bits (328), Expect = 5.2e-27
Identity = 59/88 (67.05%), Postives = 77/88 (87.50%), Query Frame = 0


Query: 1  MCDASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIV 60
          MCDAS+ VV A+LGQ+K+K++HPIYYASKTL   Q+NYTTT+KE+LA+VF ++KFR+Y +
Sbjct: 3  MCDASNHVVGAVLGQRKEKIMHPIYYASKTLNSFQENYTTTEKEMLAIVFAVDKFRAYFI 62

Query: 61 GSKVTVHSDHSVIRFLMAKKDTKPWLIR 89
          GSKVT++SDHS I++LM KKD KP LIR
Sbjct: 63 GSKVTIYSDHSAIKYLMVKKDAKPRLIR 90

[Close]

BLAST of PI0004274.1 vs. NCBI nr
Match: KAA0062295.1 (hypothetical protein E6C27_scaffold154G00380 [Cucumis melo var. makuwa] >TYK26691.1 hypothetical protein E5676_scaffold313G003510 [Cucumis melo var. makuwa])

HSP 1 Score: 130.6 bits (327), Expect = 6.8e-27
Identity = 64/87 (73.56%), Postives = 74/87 (85.06%), Query Frame = 0


Query: 1   MCDASDMVVEAMLGQKKDKVIHPIYYASKTLTDVQKNYTTTKKELLAVVFTIEKFRSYIV 60
           MCDASD+V+ AMLGQK DKVI+PIY  SKTL + Q+NYTTT+KELLAVVF IEK+ SYIV
Sbjct: 30  MCDASDVVIGAMLGQKNDKVIYPIYNMSKTLNEAQENYTTTEKELLAVVFAIEKYVSYIV 89

Query: 61  GSKVTVHSDHSVIRFLMAKKDTKPWLI 88
           GSKVT+HSDH  IR+LM KKD +P LI
Sbjct: 90  GSKVTIHSDHFAIRYLMVKKDAQPQLI 116

The following BLAST results are available for this feature:

BLAST of PI0004274.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P04323	1.7e-09	39.53	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast...	[more]
P20825	1.9e-08	39.24	Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste...	[more]
Q8I7P9	2.8e-07	31.25	Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast...	[more]
P10394	1.2e-05	34.88	Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaste...	[more]
P10401	1.5e-05	33.33	Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogas...	[more]

BLAST of PI0004274.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DS34	2.7e-29	76.14	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. mak...	[more]
A0A5A7V586	2.7e-29	76.14	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. mak...	[more]
A0A5A7V8R9	3.3e-27	73.56	RT_RNaseH domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A2G9HH15	5.6e-27	68.18	Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_10756 PE=...	[more]
A0A6J1DHH9	9.5e-27	67.05	LOW QUALITY PROTEIN: uncharacterized protein LOC111020110 OS=Momordica charantia...	[more]

BLAST of PI0004274.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0062414.1	5.5e-29	76.14	Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuw...	[more]
TYK26567.1	5.5e-29	76.14	Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuw...	[more]
XP_017970395.1	4.0e-27	71.59	PREDICTED: uncharacterized protein LOC108660654 [Theobroma cacao]	[more]
WP_217833163.1	5.2e-27	67.05	Ty3/Gypsy family RNase HI domain-containing protein, partial [Synechococcus sp. ...	[more]
KAA0062295.1	6.8e-27	73.56	hypothetical protein E6C27_scaffold154G00380 [Cucumis melo var. makuwa] >TYK2669...	[more]

BLAST of PI0004274.1 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.20.370		coord: 1..68 e-value: 7.0E-10 score: 40.7
None	No IPR available	CDD	cd09274	RNase_HI_RT_Ty3	coord: 1..88 e-value: 2.07386E-36 score: 117.208
IPR041373	Reverse transcriptase, RNase H-like domain	PFAM	PF17917	RT_RNaseH	coord: 2..86 e-value: 5.3E-25 score: 87.8
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 1..87

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
PI0004274	PI0004274	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0004274.1-exon	PI0004274.1-exon-chr01:20299000..20299266	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0004274.1-cds	PI0004274.1-cds-chr01:20299000..20299266	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
PI0004274.1	PI0004274.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0003676	nucleic acid binding