PI0003296.1 (mRNA) Melon (PI 482460) v1

Overview


Name	PI0003296.1
Type	mRNA
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Pectin lyase-like superfamily protein
Location	chr09: 1480487 .. 1484061 (+)
Sequence length	1713
RNA-Seq Expression	PI0003296.1
Synteny	PI0003296.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGATTCTTCTCTTTCTTCCTCACCATTTTCTTCCATTAATTAACAAAAATCCTCAAATCTTTTCTCCATTTCCACTTCCTTCTTGGATCTTTCTCCTTGTTTTCACTCTCAGATCTACTCCCCTTCGTCCCATCCTTCTTCTTCCTCAACTTCTTCTTTAAATCCTTACTTAATTCCCCCCAAAAAGCTTCTCTCAACTCCTTTCTCCTTGTTTTCTTTCCCATTTCTTCACAATGCTCCCCTTTTCCCTCTTCTTTTTCATCTTCTTCACTACCCTTTTCCCCTCTCCACTCGCCGTCGCCGACCTCACTACCTGCTCCGGTATTGTCCCCATGAAGTACAGGAACGACAAGATTTCCATTGCCGACTTTGGTGGAATCGGCGATGGCCGAACATTGAACACCAGAGCCTTTCGAGCTGCCATTTATCGGATTCAGCATTTAAGGAGAAGAGGGGGTACTTTGCTTTACATTCCTCCGGGGGTTTACTTGACCGAGAGCTTCAATCTCACTAGCCATATGACTCTCTATCTTGCTAAAGGCGCTGTTATCAAAGCTGTTCAGGTATTTACATCTCTCTCAAGTCTCAACTGAACTGAACTTCATTCTTCTTTTCCTAGTTTGTGATTTGTGTGGTTGTTCATGTTTAGGGTCTGTGGGTGTGTGTGTTTTTTTAATCGTCAATTCTGATATTGAGCTTGTTTTTGTTGGAGATTGGTATGAAATCTACATTCAAATAAAACAGAAAATGTAAACGGCAGAGAATTGACACAGAGATTACATGGTTGACTAACAGTGTATGTTCATGGCAGTGAGAGAACAATTCTCAATTTATTATTATTGGAGTGAAAATCTTATATTACAGATTTAGGAGGACCAGTAGTGTTATAGAATTTCTATATCGCACTCTTCTTAAACTCTAGAACCAGAGAAGTAAATAACATAAATAATGTCCCATAACAAATTCAAAACATTCATAATGATAATGGTTTCTAAATTAGGGGATTGGGATTTATGAGAATGAAAGTGGGTCCTGAATGGAAATTGCAAGAAGTGGTGGGTGGGTCTTTGATTTAAGCTGTGGCATGTGCATCCACTCTAAATTAATACTATTATATATGATTGGACCCTTGACAGGCTGTTCTTTGTCAGCCTCTGATGCTAACTTTAATTAGAATGTGATCCCGTTTTAGATGATTGGTCAATTCATAGCTCAAAATTGCTAATTTGTGATTGTTATTCTACTGATCTGGACATCAATTTCATGCAAACAAAATTGGTTGTCAGTTAGTTCATGTTCAAATATCTAATGTGTAAACTATGTCAAAATATTGGTGGGTTCTATTTAAATTACAATGATAATGACAACGTTGTTAACGAGTGATAGAAATTGTCTTAAATTTGTTAGGATAGCACATAACAAGAAACTCAAGAAGCACACAGATAGTTATTTGTAGCAATACCAAGAGAAGTTACGATATACTATAGCCTTTCTAGAGGCCTACTTAGTCTCTCCAAGTCCCACAATGGAAGCTTTCACCAAAAATAGTCTCCTAAGGCCCCTATCTCCCTTTGTTTAAGATCAAGTCTCTCCTAGCTAACCCATATGCTACCCTCACAACATCATGTTTATTTCTGCTAGCAGAAAGAGAAAAAGTGGTCTAATTTGTGCTATTTGATCTTTTTGAAATTAGAACATCAAGAAATTTATGCTGGTTTTTGAAATTAGAAACTGTGTATATGTTGTTAGGGCCTAAAAATTGACTCCTCTATGGTTGCTACTTACTAGCCTCCTATAAACGTTCTTTTTCTGGTGGGTCTGTGTGTATGCTAATGGACGGTCAATTTAGTGGTTACTAGATCCTGGGTCTAAAATATTTCACCATTTGTACTGTTGTCTCTACTATCCTTCCCATGAAATGATTTGCCGATGATCATGAGACTTGTCCGACCTCTGTAAATTTTCTCTTATGCTAAATAGCTGAAGATTCTTTTACTTTTACTTGCATTATGCAAATTTCTTTAGTAAGCATGTTGTTTTCGATTGATTGTCCAGGATTCTTCAAATTGGCCAGTAATAGCTCCGTTGCCGTCTTATGGAAGAGGGAGAGAGCGCCCTGGAGGAAGATATGTAAGCTTTATTCATGGAGATGGTGTTCATGATGTGGTGATCACTGGTATGAATCATCAAATTCTTTTAAATATGAAAGACATCGCAACCTTGGCTTACCTGTGCTGTTTTTGGAAAAGTTATGGATATTGATAGCCTGAGTAAAATTGTTATCTTAGGTGAGAATGGGACTATTGATGGCCAAGGTGATGCCTGGTGGAATATGTGGAGGACAGGAACTCTGAAGTACACTAGGCCTAATCTTGTTGAATTTATCAATTCATATAATATCATCATATCCAATGTAATGTTCCTGAATTCTCCGTTTTGGAACATTCACCCTGTTTACTGCAGGTAAGAATTAAAGTCTTATCTTCAATCTTGTTACAGCTCCATATAATTGCTCCTAACTCAGTTGTATAGTTCTATCTTACATTTTAATCTTTTATTGCAGCAACGTTGTTGTCAGATATGTCACAATATTGGCTCCTCGTGATTCCCCCAACACTGATGGAGTTGATCCAGGTGCACTTTCGGATCCTTCGGTGCTTCGTTGTTCTGATTACAAGAGCTAAACAAACTTGTTAAAACAAATGCTTATTGTAAATCATGTTCAAAATTCTCATTATATTAGCTTTGTATATCCTTGAGAGTAACAAAACTTGCAAACTGATGCTCCACAGATTCAAGCAATAACGTTTGCATAGAAGATTCCTACATTTCAACTGGAGATGATCTTGTAGCTGTGAAGAGTGGGTGGGATGAATATGGTATTGCTTATGGTCGCAGTAGTTACGACATTACCATCCGAAGAATTTCGGGGTCTAGTCCATTTGCCGGAGTTGCAGTTGGAAGTGAAACCTCTGGTGGAGTGGCAAATGTATTGGCAGAACACCTGAACTTTTACGACATGGGAGTTGGTATCAACATCAAGACAAACATCGGAAGGGGAGGGTTCATAAAGAACATAACTGTGTCCAATGTCTACATGGAGAATTCAAGAAAGGGTCTAAAGATTGCTGGCGATACCGGAGATCATCCCGATGACAAGTTCGACCCAAATGCTCTTCCTATTGTCAAAGACATCACGATTAAAAATGTTTGGGGCGTGAAAGTACAGCAAGCAGGTTCTATATATGGCTTGAGAGACTCTCCATTTACTGGTATATGTCTTTCGAATATCAACCTTCGCGGTGCAACAAGACCGAGATCAGTACCATGGACTTGCTCATATGTAAGTGGTGCAGCTCTTTTGGTTAGTCCATGGCCGTGCTCGGAGCTCACCAGCACAGAGCAAGATGGCCTGTGTTCTGATAACTTCTAAATACATGTACTTTGTATATTTGTTCTTTAAACTTGTGAAATTTTCACTTTCTCAGTTTGTAGCATCTATATTTCATTTGTCTCGAAAGTGTTTATGATGATGTTCAATATCATAATGGAAGTATCGAATATATTAATTTTT

mRNA sequence

CTGATTCTTCTCTTTCTTCCTCACCATTTTCTTCCATTAATTAACAAAAATCCTCAAATCTTTTCTCCATTTCCACTTCCTTCTTGGATCTTTCTCCTTGTTTTCACTCTCAGATCTACTCCCCTTCGTCCCATCCTTCTTCTTCCTCAACTTCTTCTTTAAATCCTTACTTAATTCCCCCCAAAAAGCTTCTCTCAACTCCTTTCTCCTTGTTTTCTTTCCCATTTCTTCACAATGCTCCCCTTTTCCCTCTTCTTTTTCATCTTCTTCACTACCCTTTTCCCCTCTCCACTCGCCGTCGCCGACCTCACTACCTGCTCCGGTATTGTCCCCATGAAGTACAGGAACGACAAGATTTCCATTGCCGACTTTGGTGGAATCGGCGATGGCCGAACATTGAACACCAGAGCCTTTCGAGCTGCCATTTATCGGATTCAGCATTTAAGGAGAAGAGGGGGTACTTTGCTTTACATTCCTCCGGGGGTTTACTTGACCGAGAGCTTCAATCTCACTAGCCATATGACTCTCTATCTTGCTAAAGGCGCTGTTATCAAAGCTGTTCAGGATTCTTCAAATTGGCCAGTAATAGCTCCGTTGCCGTCTTATGGAAGAGGGAGAGAGCGCCCTGGAGGAAGATATGTAAGCTTTATTCATGGAGATGGTGTTCATGATGTGGTGATCACTGGTGAGAATGGGACTATTGATGGCCAAGGTGATGCCTGGTGGAATATGTGGAGGACAGGAACTCTGAAGTACACTAGGCCTAATCTTGTTGAATTTATCAATTCATATAATATCATCATATCCAATGTAATGTTCCTGAATTCTCCGTTTTGGAACATTCACCCTGTTTACTGCAGCAACGTTGTTGTCAGATATGTCACAATATTGGCTCCTCGTGATTCCCCCAACACTGATGGAGTTGATCCAGATTCAAGCAATAACGTTTGCATAGAAGATTCCTACATTTCAACTGGAGATGATCTTGTAGCTGTGAAGAGTGGGTGGGATGAATATGGTATTGCTTATGGTCGCAGTAGTTACGACATTACCATCCGAAGAATTTCGGGGTCTAGTCCATTTGCCGGAGTTGCAGTTGGAAGTGAAACCTCTGGTGGAGTGGCAAATGTATTGGCAGAACACCTGAACTTTTACGACATGGGAGTTGGTATCAACATCAAGACAAACATCGGAAGGGGAGGGTTCATAAAGAACATAACTGTGTCCAATGTCTACATGGAGAATTCAAGAAAGGGTCTAAAGATTGCTGGCGATACCGGAGATCATCCCGATGACAAGTTCGACCCAAATGCTCTTCCTATTGTCAAAGACATCACGATTAAAAATGTTTGGGGCGTGAAAGTACAGCAAGCAGGTTCTATATATGGCTTGAGAGACTCTCCATTTACTGGTATATGTCTTTCGAATATCAACCTTCGCGGTGCAACAAGACCGAGATCAGTACCATGGACTTGCTCATATGTAAGTGGTGCAGCTCTTTTGGTTAGTCCATGGCCGTGCTCGGAGCTCACCAGCACAGAGCAAGATGGCCTGTGTTCTGATAACTTCTAAATACATGTACTTTGTATATTTGTTCTTTAAACTTGTGAAATTTTCACTTTCTCAGTTTGTAGCATCTATATTTCATTTGTCTCGAAAGTGTTTATGATGATGTTCAATATCATAATGGAAGTATCGAATATATTAATTTTT

Coding sequence (CDS)

ATGCTCCCCTTTTCCCTCTTCTTTTTCATCTTCTTCACTACCCTTTTCCCCTCTCCACTCGCCGTCGCCGACCTCACTACCTGCTCCGGTATTGTCCCCATGAAGTACAGGAACGACAAGATTTCCATTGCCGACTTTGGTGGAATCGGCGATGGCCGAACATTGAACACCAGAGCCTTTCGAGCTGCCATTTATCGGATTCAGCATTTAAGGAGAAGAGGGGGTACTTTGCTTTACATTCCTCCGGGGGTTTACTTGACCGAGAGCTTCAATCTCACTAGCCATATGACTCTCTATCTTGCTAAAGGCGCTGTTATCAAAGCTGTTCAGGATTCTTCAAATTGGCCAGTAATAGCTCCGTTGCCGTCTTATGGAAGAGGGAGAGAGCGCCCTGGAGGAAGATATGTAAGCTTTATTCATGGAGATGGTGTTCATGATGTGGTGATCACTGGTGAGAATGGGACTATTGATGGCCAAGGTGATGCCTGGTGGAATATGTGGAGGACAGGAACTCTGAAGTACACTAGGCCTAATCTTGTTGAATTTATCAATTCATATAATATCATCATATCCAATGTAATGTTCCTGAATTCTCCGTTTTGGAACATTCACCCTGTTTACTGCAGCAACGTTGTTGTCAGATATGTCACAATATTGGCTCCTCGTGATTCCCCCAACACTGATGGAGTTGATCCAGATTCAAGCAATAACGTTTGCATAGAAGATTCCTACATTTCAACTGGAGATGATCTTGTAGCTGTGAAGAGTGGGTGGGATGAATATGGTATTGCTTATGGTCGCAGTAGTTACGACATTACCATCCGAAGAATTTCGGGGTCTAGTCCATTTGCCGGAGTTGCAGTTGGAAGTGAAACCTCTGGTGGAGTGGCAAATGTATTGGCAGAACACCTGAACTTTTACGACATGGGAGTTGGTATCAACATCAAGACAAACATCGGAAGGGGAGGGTTCATAAAGAACATAACTGTGTCCAATGTCTACATGGAGAATTCAAGAAAGGGTCTAAAGATTGCTGGCGATACCGGAGATCATCCCGATGACAAGTTCGACCCAAATGCTCTTCCTATTGTCAAAGACATCACGATTAAAAATGTTTGGGGCGTGAAAGTACAGCAAGCAGGTTCTATATATGGCTTGAGAGACTCTCCATTTACTGGTATATGTCTTTCGAATATCAACCTTCGCGGTGCAACAAGACCGAGATCAGTACCATGGACTTGCTCATATGTAAGTGGTGCAGCTCTTTTGGTTAGTCCATGGCCGTGCTCGGAGCTCACCAGCACAGAGCAAGATGGCCTGTGTTCTGATAACTTCTAA

Protein sequence

MLPFSLFFFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLVEFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVLAEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGAALLVSPWPCSELTSTEQDGLCSDNF

Homology

[Close]

BLAST of PI0003296.1 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.4e-115
Identity = 203/409 (49.63%), Postives = 273/409 (66.75%), Query Frame = 0


Query: 37  RNDKISIADFGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHM 96
           R    S+ DFGG+GDG+TLNT+AF+ A+  +      GG  LY+P G +LT SF+LTSH 
Sbjct: 59  RAHSASLVDFGGVGDGQTLNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHF 118

Query: 97  TLYLAKGAVIKAVQDSSNWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTI 156
           TL+L + AV+ A QD S WPVI PLPSYGRGR+   GRY S I G  + DV+ITG+NGTI
Sbjct: 119 TLFLHRDAVLLASQDISQWPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTI 178

Query: 157 DGQGDAWWNMWRTGTLKYTRPNLVEFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYV 216
           DGQG  WW  +  G LKYTRP L+E + S +I ISN+  LNSP WN+HPVY  N++++ +
Sbjct: 179 DGQGGLWWQRFHGGKLKYTRPYLIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGI 238

Query: 217 TILAPRDSPNTDGVDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR 276
           TILAP  SPNTDG++PDS  N  IED YI +GDD VAVKSGWDEYGIAYG  +  + IRR
Sbjct: 239 TILAPVRSPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRR 298

Query: 277 ISGSSPFAGV-AVGSETSGGVANVLAEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYM 336
           ++  SP++ V A+GSE SGG+ +V AE +   +   GI IKT IGRGG++K+I V  + M
Sbjct: 299 LTCISPYSAVIALGSEMSGGIQDVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTM 358

Query: 337 ENSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGIC 396
           +  +    + G+ G H D+ +DP A P+++ I  +++    V  A  + G+   PFTGIC
Sbjct: 359 KTMKWAFWMTGNYGSHADNHYDPKAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGIC 418

Query: 397 LSNINLRGATRPRSVPWTCSYVSGAALLVSPWPCSEL--TSTEQDGLCS 443
           +SN+ +  A + + VPWTC+ V G +  V+P PCS L     E+  LC+
Sbjct: 419 ISNVTIHLAAKAKKVPWTCTDVEGISSGVTPTPCSTLPDQGPEKTSLCN 467

[Close]

BLAST of PI0003296.1 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 132.1 bits (331), Expect = 1.5e-29
Identity = 104/375 (27.73%), Postives = 176/375 (46.93%), Query Frame = 0


Query: 41  ISIADFGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPG-VYLTESFNLTS--HMT 100
           +S++DFG  GDG+T +T+AF  A  +       G   L +P G  YL +S  LT   +  
Sbjct: 68  VSVSDFGAKGDGKTDDTQAFVNAWKKA--CSSNGAVNLLVPKGNTYLLKSIQLTGPCNSI 127

Query: 101 LYLAKGAVIKAVQDSSNWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVIT-GENGTI 160
           L +     + A Q  S++  I+              +++ F   DGV+++ +  G+ G +
Sbjct: 128 LTVQIFGTLSASQKRSDYKDIS--------------KWIMF---DGVNNLSVDGGDTGVV 187

Query: 161 DGQGDAWW-NMWRTGTLK--YTRPNLVEFINSYNIIISNVMFLNSPFWNIHPVYCSNVVV 220
           DG G+ WW N  +    K     P  + F NS ++I+ N+   N+    I    CSNV V
Sbjct: 188 DGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQV 247

Query: 221 RYVTILAPRDSPNTDGVDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDIT 280
             V + AP DSPNTDG+   ++ N+ + +S I TGDD ++++SG           S ++ 
Sbjct: 248 SNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------SQNVQ 307

Query: 281 IRRISGSSPFAGVAVGS----ETSGGVANVLAEHLNFYDMGVGINIKTNIGRGGFIKNIT 340
           I  I+   P  G+++GS     +   V+ V  +         G+ IKT  G  G   NI 
Sbjct: 308 INDIT-CGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNII 367

Query: 341 VSNVYMENSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVKVQQAGSIYGL-RD 400
             N+ M+N +  + I  D  D      + +A+  VK++  +++ G    +    +   ++
Sbjct: 368 FQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAVQ-VKNVVYRDISGTSASENAITFNCSKN 410

Query: 401 SPFTGICLSNINLRG 404
            P  GI L  +N++G
Sbjct: 428 YPCQGIVLDRVNIKG 410

[Close]

BLAST of PI0003296.1 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 3.3e-24
Identity = 112/413 (27.12%), Postives = 180/413 (43.58%), Query Frame = 0


Query: 38  NDKISIADFGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMT 97
           ++ + +  FG +GDG T +++AF  A   +      G     +P G            MT
Sbjct: 21  SNALDVTQFGAVGDGVTDDSQAFLKAWEAV--CSGTGDGQFVVPAG------------MT 80

Query: 98  LYLAKGAVIKAVQDSSNWPVIAPL------PSYGRGRERPGGRYVSFIHGDGVHDVVITG 157
             L     +K      + PV   +      PS G  +    G    +I    +  +VI G
Sbjct: 81  FMLQP---LKFQGSCKSTPVFVQMLGKLVAPSKGNWK----GDKDQWILFTDIEGLVIEG 140

Query: 158 ENGTIDGQGDAWWNMWRTGTLKYTRPNLVEFINSYNIIISNVMFLNSPFWNIHPVYCSNV 217
           + G I+GQG +WW        K +RP  ++F +  N+ +S +  L+SP  +IH   C+ V
Sbjct: 141 D-GEINGQGSSWWEH------KGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYV 200

Query: 218 VVRYVTILAPRDSPNTDGVDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYD 277
            +  + I AP  SPNTDG+D  +S+NV I+D  I+TGDD +A+ SG     I    S  D
Sbjct: 201 TISSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHI----SGID 260

Query: 278 ITIRRISGSSPFAGVAVGSETSGG----VANVLAEHLNFYDMGVGINIKTNIGRGGFIKN 337
                     P  G+++GS    G    V NV  ++ NF     G  IKT  G  G+ + 
Sbjct: 261 --------CGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARM 320

Query: 338 ITVSNVYMENSRKGLKIAG--DTGDHPDDKFDPNALPIVKDITIKNVWGVKVQQAGSIYG 397
           IT + + ++N    + I    + GD  + K   ++   V  +   N  G    + G  + 
Sbjct: 321 ITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFR 380

Query: 398 LRDS-PFTGICLSNINLRGAT--RPRSVPWTCSYVSGAALLVSP-WPCSELTS 435
             +  P T I L ++ +  A+    +     C  V GA+ +  P   C EL++
Sbjct: 381 CSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVPGLECLELST 393

[Close]

BLAST of PI0003296.1 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 5.7e-24
Identity = 98/375 (26.13%), Postives = 162/375 (43.20%), Query Frame = 0


Query: 41  ISIADFGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYL 100
           +S+++FG  GDG+T +T+AF+ A  +       G T   +P G               YL
Sbjct: 68  VSVSNFGAKGDGKTDDTQAFKKAWKKA--CSTNGVTTFLVPKG-------------KTYL 127

Query: 101 AKGAVIKA-VQDSSNWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVIT-GENGTIDG 160
            K    +   +   N+ ++  L +  +  +     +   +  + V+++ I  G  G I+G
Sbjct: 128 LKSTRFRGPCKSLRNFQILGTLSASTKRSDYKDKNHWLIL--EDVNNLSIDGGSTGIING 187

Query: 161 QGDAWW-NMWRTGTLK--YTRPNLVEFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRY 220
            G  WW N  +    K     P  +   N  N+ + N+   N+    I    C+ V V  
Sbjct: 188 NGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSN 247

Query: 221 VTILAPRDSPNTDGVDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIR 280
           V I AP DSPNTDG+   ++ N+ + +S I TGDD ++++ G     I      +D+T  
Sbjct: 248 VEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQI------FDLT-- 307

Query: 281 RISGSSPFAGVAVGS----ETSGGVANVLAEHLNFYDMGVGINIKTNIGRGGFIKNITVS 340
                 P  G+++GS     +   V+ +  +   F +   G+ IKT  G  G  KNI   
Sbjct: 308 ----CGPGHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQ 367

Query: 341 NVYMENSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVKVQQAGSIYGLRDS-P 400
           N+ MEN +  + I  D  D  D   D  +   VK++  KN+ G             +  P
Sbjct: 368 NIRMENVKNPIIIDQDYCD-KDKCEDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYP 412

Query: 401 FTGICLSNINLRGAT 406
             GI L N+ ++G T
Sbjct: 428 CQGIVLENVKIKGGT 412

[Close]

BLAST of PI0003296.1 vs. ExPASy Swiss-Prot
Match: P18192 (Endo-polygalacturonase OS=Pectobacterium carotovorum subsp. carotovorum OX=555 GN=peh PE=3 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 3.4e-21
Identity = 97/304 (31.91%), Postives = 146/304 (48.03%), Query Frame = 0


Query: 84  VYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAPLPSYGRGRERPGGRYVSFIHGDG 143
           V+L+   +L S ++L + KG  ++AV ++ ++      PS     +  G    +FI    
Sbjct: 77  VFLSGPLSLPSGVSLLIDKGVTLRAVNNAKSF---ENAPSSCGVVDTNGKGCDAFITATS 136

Query: 144 VHDVVITGENGTIDGQGD--------AWWNM---WRTGTLKYTRPNLVEFINSYNIIISN 203
             +  I G  GTIDGQG         +WW++    +   LK   P L++   S N  + N
Sbjct: 137 TTNSGIYGP-GTIDGQGGVKLQDKKVSWWDLAADAKVKKLKQNTPRLIQINKSKNFTLYN 196

Query: 204 VMFLNSPFWNIHPVYC--SNVVVRYVTILAPRDSPNTDGVDPDSSNNVCIEDSYISTGDD 263
           V  +NSP  N H V+           TI  P  + NTDG+DP SS N+ I  S ISTGDD
Sbjct: 197 VSLINSP--NFHVVFSDGDGFTAWKTTIKTPSTARNTDGIDPMSSKNITIAHSNISTGDD 256

Query: 264 LVAVKSGWDEYGIAY-GRS-SYDITIRRISGSSPFAGVAVGSETSGGVANVLAEHLNFYD 323
            VA+K        AY GRS + +I+I      +   G+++GSET  GV NV  + L    
Sbjct: 257 NVAIK--------AYKGRSETRNISILHNEFGTGH-GMSIGSETM-GVYNVTVDDLIMTG 316

Query: 324 MGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNALPIVKDIT 373
              G+ IK++    G +  +  SNV M+N  K + I     D   +K + + +P   DIT
Sbjct: 317 TTNGLRIKSDKSAAGVVNGVRYSNVVMKNVAKPIVI-----DTVYEKKEGSNVPDWSDIT 359

[Close]

BLAST of PI0003296.1 vs. ExPASy TrEMBL
Match: A0A5A7TLS6 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001910 PE=3 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 3.5e-258
Identity = 436/445 (97.98%), Postives = 439/445 (98.65%), Query Frame = 0


Query: 1   MLPFSLFFFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           M+PFSLF FIFFTTLFPSPLAVADL TCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF
Sbjct: 1   MVPFSLFVFIFFTTLFPSPLAVADLITCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTE+FNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHG GVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGHGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240
           EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420
           LPIVKDITIKN WGVKVQQAGSIYGLRDSPFTGICLSNIN RG TRPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNAWGVKVQQAGSIYGLRDSPFTGICLSNINFRGTTRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTEQDGLCSDNF 446
           AL+VSPWPCSELTSTEQDGLCSDNF
Sbjct: 421 ALMVSPWPCSELTSTEQDGLCSDNF 445

[Close]

BLAST of PI0003296.1 vs. ExPASy TrEMBL
Match: A0A1S3ATJ9 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482817 PE=3 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 3.5e-258
Identity = 436/445 (97.98%), Postives = 439/445 (98.65%), Query Frame = 0


Query: 1   MLPFSLFFFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           M+PFSLF FIFFTTLFPSPLAVADL TCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF
Sbjct: 1   MVPFSLFVFIFFTTLFPSPLAVADLITCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTE+FNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHG GVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGHGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240
           EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420
           LPIVKDITIKN WGVKVQQAGSIYGLRDSPFTGICLSNIN RG TRPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNAWGVKVQQAGSIYGLRDSPFTGICLSNINFRGTTRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTEQDGLCSDNF 446
           AL+VSPWPCSELTSTEQDGLCSDNF
Sbjct: 421 ALMVSPWPCSELTSTEQDGLCSDNF 445

[Close]

BLAST of PI0003296.1 vs. ExPASy TrEMBL
Match: A0A0A0KK36 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G150430 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.2e-255
Identity = 430/445 (96.63%), Postives = 436/445 (97.98%), Query Frame = 0


Query: 1   MLPFSLFFFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           MLPF LF FIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF
Sbjct: 1   MLPFPLFIFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTE+FNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180
           LPSYGRGRERPGGRY+S IHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRP+LV
Sbjct: 121 LPSYGRGRERPGGRYISLIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPSLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240
           EF+NSYNIIISNVMFLNSPFWNIHPVYC NVVVRYVTILAPRDSPNTDGVDPDSSNNVCI
Sbjct: 181 EFVNSYNIIISNVMFLNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGR SYDITIRRISGSSPFAGVAVGSE SGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRCSYDITIRRISGSSPFAGVAVGSEASGGVANVL 300

Query: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGD GDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDAGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420
           LPIVKDITIKN+WGV VQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNIWGVNVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTEQDGLCSDNF 446
           A LVSPWPCSELTST+QDGLCSDNF
Sbjct: 421 ASLVSPWPCSELTSTDQDGLCSDNF 445

[Close]

BLAST of PI0003296.1 vs. ExPASy TrEMBL
Match: A0A6J1I1Q1 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111469049 PE=3 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 2.5e-248
Identity = 417/444 (93.92%), Postives = 430/444 (96.85%), Query Frame = 0


Query: 1   MLPFSLFFFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           MLPFSLF FI+F TL PSP A+A LTTCSGIVPM YRNDKISI DFGGIGDGRTLNT+AF
Sbjct: 1   MLPFSLFIFIYFATLLPSPFAIAQLTTCSGIVPMNYRNDKISITDFGGIGDGRTLNTKAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQ+L+RRGGTLLYIPPGVYLTESFNLTSHMTLYLAK AVIKAVQ SSNWPV+AP
Sbjct: 61  RAAIYRIQNLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQGSSNWPVVAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLK+TRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240
           EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDG+DPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFAGVAVGSETSGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHLNFYDMG+GINIKTNIGRGGFIKNITVSNVYME SRKGLKIAGDTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGIGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWGV VQQAGSIYGLR+SPFTGICLSNI+LRGA RPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNIHLRGAMRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTEQDGLCSDN 445
           ALLVSPWPCSELTST+QDG CS N
Sbjct: 421 ALLVSPWPCSELTSTQQDGSCSAN 444

[Close]

BLAST of PI0003296.1 vs. ExPASy TrEMBL
Match: A0A6J1EMW9 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111435810 PE=3 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 1.0e-246
Identity = 415/444 (93.47%), Postives = 427/444 (96.17%), Query Frame = 0


Query: 1   MLPFSLFFFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           MLPFSLF FI   TL PSP A+A LTTCSGIVPM YRNDKISI DFGGIGDGRTLNT+AF
Sbjct: 1   MLPFSLFIFIHLATLLPSPFAIAQLTTCSGIVPMNYRNDKISITDFGGIGDGRTLNTKAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHL+RRGGTLLYIPPGVYLTESFNLTSHMTLYLAK AVIKAVQ SSNWPV+AP
Sbjct: 61  RAAIYRIQHLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQGSSNWPVVAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLK+TRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240
           EFINSY+IIISNV FLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDG+DPDSSNNVCI
Sbjct: 181 EFINSYDIIISNVRFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFAGVAVGSETSGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHLNFYDMG+GINIKTNIGRGGFIKNITVSNVYME SRKGLKIAGDTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGIGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWGV VQQAGSIYGLR+SPFTGICLSNI+LRGATRPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNIHLRGATRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTEQDGLCSDN 445
           ALLVSPWPCS LTST+QDG CS N
Sbjct: 421 ALLVSPWPCSALTSTQQDGSCSAN 444

[Close]

BLAST of PI0003296.1 vs. NCBI nr
Match: XP_008437386.1 (PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042687.1 putative polygalacturonase [Cucumis melo var. makuwa] >TYK06090.1 putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 900.2 bits (2325), Expect = 7.1e-258
Identity = 436/445 (97.98%), Postives = 439/445 (98.65%), Query Frame = 0


Query: 1   MLPFSLFFFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           M+PFSLF FIFFTTLFPSPLAVADL TCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF
Sbjct: 1   MVPFSLFVFIFFTTLFPSPLAVADLITCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTE+FNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHG GVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGHGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240
           EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420
           LPIVKDITIKN WGVKVQQAGSIYGLRDSPFTGICLSNIN RG TRPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNAWGVKVQQAGSIYGLRDSPFTGICLSNINFRGTTRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTEQDGLCSDNF 446
           AL+VSPWPCSELTSTEQDGLCSDNF
Sbjct: 421 ALMVSPWPCSELTSTEQDGLCSDNF 445

[Close]

BLAST of PI0003296.1 vs. NCBI nr
Match: XP_004143871.1 (probable polygalacturonase [Cucumis sativus] >KGN50025.1 hypothetical protein Csa_000391 [Cucumis sativus])

HSP 1 Score: 891.7 bits (2303), Expect = 2.5e-255
Identity = 430/445 (96.63%), Postives = 436/445 (97.98%), Query Frame = 0


Query: 1   MLPFSLFFFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           MLPF LF FIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF
Sbjct: 1   MLPFPLFIFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTE+FNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180
           LPSYGRGRERPGGRY+S IHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRP+LV
Sbjct: 121 LPSYGRGRERPGGRYISLIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPSLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240
           EF+NSYNIIISNVMFLNSPFWNIHPVYC NVVVRYVTILAPRDSPNTDGVDPDSSNNVCI
Sbjct: 181 EFVNSYNIIISNVMFLNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGR SYDITIRRISGSSPFAGVAVGSE SGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRCSYDITIRRISGSSPFAGVAVGSEASGGVANVL 300

Query: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGD GDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDAGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420
           LPIVKDITIKN+WGV VQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNIWGVNVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTEQDGLCSDNF 446
           A LVSPWPCSELTST+QDGLCSDNF
Sbjct: 421 ASLVSPWPCSELTSTDQDGLCSDNF 445

[Close]

BLAST of PI0003296.1 vs. NCBI nr
Match: XP_038876129.1 (probable polygalacturonase [Benincasa hispida])

HSP 1 Score: 885.6 bits (2287), Expect = 1.8e-253
Identity = 426/445 (95.73%), Postives = 437/445 (98.20%), Query Frame = 0


Query: 1   MLPFSLFFFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           M+PF LF F +FTTLFPSPLAVADLTTCSGIVPMKYRNDKISI DFGGIGDGRTLNTRAF
Sbjct: 1   MVPFFLFIFFYFTTLFPSPLAVADLTTCSGIVPMKYRNDKISITDFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVI+AVQDSSNWPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIRAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLK+TRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240
           EFINSYNIIISNVMFLNSPFWNIHPVYC+NVVVRYVTILAPRDSPNTDGVDPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCTNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFAGVAVGSETSGGV NVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVENVL 300

Query: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHLNFYDMGVGINIKTNIGRGGFI+NITVSNVYME+SRKGLKIAGDTGDHPDDK+DPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKIAGDTGDHPDDKYDPNA 360

Query: 361 LPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWG  VQQAGSIYGLR+SPFTGICLSNINLRGA RPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGARRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTEQDGLCSDNF 446
           ALLVSPWPCSELTST+QDGLCSDNF
Sbjct: 421 ALLVSPWPCSELTSTQQDGLCSDNF 445

[Close]

BLAST of PI0003296.1 vs. NCBI nr
Match: XP_022970040.1 (probable polygalacturonase [Cucurbita maxima] >XP_022970041.1 probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 867.5 bits (2240), Expect = 5.1e-248
Identity = 417/444 (93.92%), Postives = 430/444 (96.85%), Query Frame = 0


Query: 1   MLPFSLFFFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           MLPFSLF FI+F TL PSP A+A LTTCSGIVPM YRNDKISI DFGGIGDGRTLNT+AF
Sbjct: 1   MLPFSLFIFIYFATLLPSPFAIAQLTTCSGIVPMNYRNDKISITDFGGIGDGRTLNTKAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQ+L+RRGGTLLYIPPGVYLTESFNLTSHMTLYLAK AVIKAVQ SSNWPV+AP
Sbjct: 61  RAAIYRIQNLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQGSSNWPVVAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLK+TRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240
           EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDG+DPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFAGVAVGSETSGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHLNFYDMG+GINIKTNIGRGGFIKNITVSNVYME SRKGLKIAGDTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGIGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWGV VQQAGSIYGLR+SPFTGICLSNI+LRGA RPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNIHLRGAMRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTEQDGLCSDN 445
           ALLVSPWPCSELTST+QDG CS N
Sbjct: 421 ALLVSPWPCSELTSTQQDGSCSAN 444

[Close]

BLAST of PI0003296.1 vs. NCBI nr
Match: XP_023550399.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 865.9 bits (2236), Expect = 1.5e-247
Identity = 416/444 (93.69%), Postives = 429/444 (96.62%), Query Frame = 0


Query: 1   MLPFSLFFFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           MLPFSLF FI+F TL PSP A+A LTTCSGIVPM YRNDKISI DFGGIGDGRTLNT+AF
Sbjct: 1   MLPFSLFIFIYFATLLPSPFAIAQLTTCSGIVPMNYRNDKISITDFGGIGDGRTLNTKAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHL+RRGGTLLYIPPGVYLTESFNLTSHMTLYLAK AVIKAVQ SSNWPV+AP
Sbjct: 61  RAAIYRIQHLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQGSSNWPVVAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLK+TRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240
           EFINSY+IIISNV FLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDG+DPDSSNNVCI
Sbjct: 181 EFINSYDIIISNVRFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFAGVAVGSETSGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHLNFYDMG+GINIKTNIGRGGFIKNITVSNVYME SRKGLKIAGDTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGIGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWGV VQQAGSIYGLR+SPFTGICLSNI+LRGA RPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNIHLRGAMRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTEQDGLCSDN 445
           ALLVSPWPCSELTST+QDG CS N
Sbjct: 421 ALLVSPWPCSELTSTQQDGSCSAN 444

[Close]

BLAST of PI0003296.1 vs. TAIR 10
Match: AT4G23820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 689.9 bits (1779), Expect = 1.4e-198
Identity = 324/435 (74.48%), Postives = 377/435 (86.67%), Query Frame = 0


Query: 12  FTTLFPSPLAVADL-TTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAFRAAIYRIQHL 71
           F+ LF S  ++ D   TCSGIVP++YR DKISI DFGG+GDGRT+NT+AFRAAIYRIQHL
Sbjct: 11  FSCLFVSSSSLGDSEATCSGIVPLRYRYDKISITDFGGVGDGRTVNTKAFRAAIYRIQHL 70

Query: 72  RRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAPLPSYGRGRER 131
           +RRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVI+AVQD+ NWP+I PLPSYGRGRE 
Sbjct: 71  KRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIRAVQDTWNWPLIDPLPSYGRGREL 130

Query: 132 PGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLVEFINSYNIII 191
           PGGRY+SFIHGDG+ DVVITG+NGTIDGQG+ WWNMWR+ TLKYTRPNL+EF +S  III
Sbjct: 131 PGGRYMSFIHGDGLRDVVITGQNGTIDGQGEVWWNMWRSRTLKYTRPNLIEFKDSKEIII 190

Query: 192 SNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCIEDSYISTGDD 251
           SNV+F NSPFWNIHPVYCSNVV+ +VTILAP+DSPNTDG+DPDSS NVCIEDSYISTGDD
Sbjct: 191 SNVIFQNSPFWNIHPVYCSNVVIHHVTILAPQDSPNTDGIDPDSSYNVCIEDSYISTGDD 250

Query: 252 LVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVLAEHLNFYDMG 311
           LVA+KSGWD+YGIAYGR S +ITIRRI+GSSPFAG+A+GSETSGG+ N++AEH+   +MG
Sbjct: 251 LVAIKSGWDQYGIAYGRPSSNITIRRITGSSPFAGIAIGSETSGGIKNIIAEHITLSNMG 310

Query: 312 VGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIK 371
           VG+NIKTNIGRGG+IKNI +S+VY++ ++ G+KIAGDTGDHPD+ ++PNALP+VK I IK
Sbjct: 311 VGVNIKTNIGRGGYIKNIKISDVYVDTAKYGIKIAGDTGDHPDENYNPNALPVVKGIHIK 370

Query: 372 NVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGAALLVSPWPCS 431
           NVWGV V+ AGSI GL+ SPFTGICLS INL G+       W CS VSG +L VSPWPCS
Sbjct: 371 NVWGVNVRNAGSIQGLKGSPFTGICLSEINLHGSLNSYKT-WKCSDVSGTSLKVSPWPCS 430

Query: 432 ELTSTEQDGLCSDNF 446
           EL +T    LCS  F
Sbjct: 431 ELRTTGGSNLCSSTF 444

[Close]

BLAST of PI0003296.1 vs. TAIR 10
Match: AT5G41870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 624.8 bits (1610), Expect = 5.4e-179
Identity = 298/443 (67.27%), Postives = 359/443 (81.04%), Query Frame = 0


Query: 3   PFSLFFFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAFRA 62
           P S+F     +T+ P  L++    TCSGIVPMK+RN+ +SI+DFG +GDG+TLNT+AF +
Sbjct: 7   PISVFVLFLLSTVLPHHLSLGAPITCSGIVPMKHRNEMLSISDFGAVGDGKTLNTKAFNS 66

Query: 63  AIYRIQHLRRRG-GTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAPL 122
           AI RI++      GTLLY+P GVYLT+SFNLTSHMTLYLA GAVIKAVQD+  WP+  PL
Sbjct: 67  AIDRIRNSNNSNEGTLLYVPRGVYLTQSFNLTSHMTLYLADGAVIKAVQDTEKWPLTDPL 126

Query: 123 PSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLVE 182
           PSYGRGRE PG RY+SFIHGDG++DVVITG NGTIDGQG+ WWNMWR GTLK+TRP L+E
Sbjct: 127 PSYGRGREHPGRRYISFIHGDGLNDVVITGRNGTIDGQGEPWWNMWRHGTLKFTRPGLIE 186

Query: 183 FINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCIE 242
           F NS NI++S+V+  NSPFW +HPVYCSNVVV +VTILAP DS NTDG+DPDSS+NVCIE
Sbjct: 187 FNNSTNILVSHVVLQNSPFWTLHPVYCSNVVVHHVTILAPTDSYNTDGIDPDSSSNVCIE 246

Query: 243 DSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVLA 302
           DSYISTGDDLVAVKSGWDEYGIAY R S DITIRRI+GSSPFAG+A+GSETSGG+ NV  
Sbjct: 247 DSYISTGDDLVAVKSGWDEYGIAYNRPSRDITIRRITGSSPFAGIAIGSETSGGIQNVTV 306

Query: 303 EHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNAL 362
           E++  Y+ G+GI+IKTNIGRGG I+ IT+S VY+E  R G+KI+GDTGDHPDDKF+ +AL
Sbjct: 307 ENITLYNSGIGIHIKTNIGRGGSIQGITISGVYLEKVRTGIKISGDTGDHPDDKFNTSAL 366

Query: 363 PIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGAA 422
           PIV+ ITIKNVWG+KV++AG + GL+DSPFT +C SN+ L G    RS  W CS V GAA
Sbjct: 367 PIVRGITIKNVWGIKVERAGMVQGLKDSPFTNLCFSNVTLTGT--KRSPIWKCSDVVGAA 426

Query: 423 LLVSPWPCSELTSTEQDGLCSDN 445
             V+P PC EL++T Q G   +N
Sbjct: 427 DKVNPTPCPELSATTQQGGSCEN 447

[Close]

BLAST of PI0003296.1 vs. TAIR 10
Match: AT4G33440.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 448.7 bits (1153), Expect = 5.3e-126
Identity = 219/412 (53.16%), Postives = 286/412 (69.42%), Query Frame = 0


Query: 24  DLTTCSGIVPMKYRNDKI--SIADFGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIP 83
           D  TCSG        ++I  SI DFGG+GDG+T NT AFR A+  ++     GG  L +P
Sbjct: 53  DPVTCSGFFNHDPFPNRIVMSITDFGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVP 112

Query: 84  PGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAPLPSYGRGRERPGGRYVSFIHG 143
            G +L+ SFNLTS+ TL+L +GA+I   +D   WP+I PLPSYGRGRERPGGR++S IHG
Sbjct: 113 EGTWLSGSFNLTSNFTLFLERGALILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHG 172

Query: 144 DGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLVEFINSYNIIISNVMFLNSPFW 203
           D + +VVITGENGTIDGQG  WW +W   TL +TR +L+E  NS+NI+ISN+  LNSPFW
Sbjct: 173 DNLTNVVITGENGTIDGQGKMWWELWWNRTLVHTRGHLIELKNSHNILISNLTLLNSPFW 232

Query: 204 NIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCIEDSYISTGDDLVAVKSGWDEY 263
            IHPVYCSNVV+R +TILAP ++PNTDG+DPDSS NVCIED YI +GDDLVAVKSGWD+Y
Sbjct: 233 TIHPVYCSNVVIRNMTILAPMNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQY 292

Query: 264 GIAYGRSSYDITIRRISGSS-PFAGVAVGSETSGGVANVLAEHLNFYDMGVGINIKTNIG 323
           G+A  R S +I IRRISG++   +GV +GSE SGG+ N+  E ++ +D   G+ IKT+ G
Sbjct: 293 GMAVARPSSNIVIRRISGTTRTCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTDKG 352

Query: 324 RGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVKVQQA 383
           RGG+I NIT +NV +E  +  ++ +  + DH DDK+DP ALP VK I I NV  +  ++A
Sbjct: 353 RGGYISNITFNNVLLEKVKVPIRFSSGSNDHSDDKWDPKALPRVKGIYISNVVSLNSRKA 412

Query: 384 GSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGAALLVSPWPCSEL 433
             + G+  + F  +CL N+ L G   P++  W C  VSG A  V P  C +L
Sbjct: 413 PMLLGVEGTSFQDVCLRNVTLLGL--PKTEKWKCKDVSGYASDVFPLSCPQL 462

[Close]

BLAST of PI0003296.1 vs. TAIR 10
Match: AT3G62110.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 448.0 bits (1151), Expect = 9.0e-126
Identity = 210/392 (53.57%), Postives = 279/392 (71.17%), Query Frame = 0


Query: 36  YRNDKISIADFGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSH 95
           +R   +SI +FG +GDG TLNT+AF+ A++ +     +GG  L++P G +LT SF+L SH
Sbjct: 38  HRPHSVSITEFGAVGDGVTLNTKAFQNALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISH 97

Query: 96  MTLYLAKGAVIKAVQDSSNWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGT 155
           +TL+L KGA I     S NWPV+ PLPSYGRGRE PG R+ S I+G  + DVVITGENGT
Sbjct: 98  LTLWLDKGATILGSTSSENWPVVDPLPSYGRGRELPGRRHRSLIYGQNLTDVVITGENGT 157

Query: 156 IDGQGDAWWNMWRTGTLKYTRPNLVEFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRY 215
           IDGQG  WW+ +R G L YTRP+LVE +NS  +IISN+ FLNSPFWNIHPVYC +VVV+ 
Sbjct: 158 IDGQGTVWWDWFRNGELNYTRPHLVELMNSTGLIISNLTFLNSPFWNIHPVYCRDVVVKN 217

Query: 216 VTILAPRDSPNTDGVDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIR 275
           +TILAP +SPNTDGVDPDSS NVCIED YI TGDDLV++KSGWDEYGI+Y R S  I I 
Sbjct: 218 LTILAPLESPNTDGVDPDSSTNVCIEDCYIVTGDDLVSIKSGWDEYGISYARPSSKIKIN 277

Query: 276 RISG-SSPFAGVAVGSETSGGVANVLAEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVY 335
           R++G ++  +G+A+GSE SGGV+ +  + L+ ++   GI IKT+ GRGG+++N+ + NV 
Sbjct: 278 RLTGQTTSSSGIAIGSEMSGGVSEIYIKDLHLFNSNTGIRIKTSAGRGGYVRNVHILNVK 337

Query: 336 MENSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGI 395
           ++N +K ++  G  G+HPD+K+DP ALP ++ IT +NV G  +  AG + G+    F  I
Sbjct: 338 LDNVKKAIRFTGKYGEHPDEKYDPKALPAIEKITFENVNGDGIGVAGLLEGIEGDVFKNI 397

Query: 396 CLSNINLRGATRPRSVPWTCSYVSGAALLVSP 427
           C  N+ LR     +  PW CS V G +  VSP
Sbjct: 398 CFLNVTLRVKKNSKKSPWECSNVRGYSQWVSP 429

[Close]

BLAST of PI0003296.1 vs. TAIR 10
Match: AT3G16850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 421.0 bits (1081), Expect = 1.2e-117
Identity = 217/435 (49.89%), Postives = 295/435 (67.82%), Query Frame = 0


Query: 2   LPFSLFFFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAFR 61
           +P +L + + FT L  S     +   C     +  R   +SI +FG +GDG+TLNT AF+
Sbjct: 3   MPVALVWLLAFTILLIS--GEGNNAICKENFKLDPRPHSVSILEFGAVGDGKTLNTIAFQ 62

Query: 62  AAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAPL 121
            A++ ++    +GG  LY+PPG +LT SFNLTSH+TL+L KGA I A  D S+W V++PL
Sbjct: 63  NAVFYLKSFADKGGAQLYVPPGKWLTGSFNLTSHLTLFLEKGATILASPDPSHWDVVSPL 122

Query: 122 PSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLVE 181
           PSYGRG E PG RY S I+GD + DVVITGENGT DGQG AWW    +G+L Y+RP+++E
Sbjct: 123 PSYGRGIELPGKRYRSLINGDNLIDVVITGENGTFDGQGAAWWEWLESGSLNYSRPHIIE 182

Query: 182 FINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCIE 241
           F++S +I+ISN+ FLN+P  NIHPVYCS + +R V I    DSP+  GV PDSS+NVCIE
Sbjct: 183 FVSSKHILISNLTFLNAPAINIHPVYCSQIHIRKVLIETSVDSPHVLGVAPDSSDNVCIE 242

Query: 242 DSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPF-AGVAVGSETSGGVANVL 301
           DS I+ G D V++KSGWD+YGI YGR +  + IR +   SP  AG++ GSE SGGV++V 
Sbjct: 243 DSTINVGHDAVSLKSGWDQYGIHYGRPTTAVHIRNLRLKSPTGAGISFGSEMSGGVSDVT 302

Query: 302 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 361
            E LN +   +G+  +T  GRGG+I+NIT+S+V + +    +   G TG HPDDKFD +A
Sbjct: 303 VERLNIHSSLIGVAFRTTRGRGGYIRNITISDVDLTSVDTAIVANGHTGSHPDDKFDRDA 362

Query: 362 LPIVKDITIKNVWGVKVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 421
           LP+V  I ++N  GV +  AG++ G+ +SPFT ICL++I+L+  TR     W CS VSG 
Sbjct: 363 LPVVTHIVMRNFTGVDIGVAGNLTGIGESPFTSICLADIHLQ--TRSEE-SWICSNVSGF 422

Query: 422 ALLVSPWPCSELTST 436
           +  VSP PC EL S+
Sbjct: 423 SDDVSPEPCQELMSS 432

The following BLAST results are available for this feature:

BLAST of PI0003296.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7PZL3	1.4e-115	49.63	Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ...	[more]
Q8RY29	1.5e-29	27.73	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]
Q9LW07	3.3e-24	27.12	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
O23147	5.7e-24	26.13	Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1	[more]
P18192	3.4e-21	31.91	Endo-polygalacturonase OS=Pectobacterium carotovorum subsp. carotovorum OX=555 G...	[more]

BLAST of PI0003296.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TLS6	3.5e-258	97.98	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3ATJ9	3.5e-258	97.98	probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482817 PE=3 SV=1	[more]
A0A0A0KK36	1.2e-255	96.63	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G150430 PE=3 SV=1	[more]
A0A6J1I1Q1	2.5e-248	93.92	probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111469049 PE=3 SV=1	[more]
A0A6J1EMW9	1.0e-246	93.47	probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111435810 PE=3 SV...	[more]

BLAST of PI0003296.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008437386.1	7.1e-258	97.98	PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042687.1 putative poly...	[more]
XP_004143871.1	2.5e-255	96.63	probable polygalacturonase [Cucumis sativus] >KGN50025.1 hypothetical protein Cs...	[more]
XP_038876129.1	1.8e-253	95.73	probable polygalacturonase [Benincasa hispida]	[more]
XP_022970040.1	5.1e-248	93.92	probable polygalacturonase [Cucurbita maxima] >XP_022970041.1 probable polygalac...	[more]
XP_023550399.1	1.5e-247	93.69	probable polygalacturonase [Cucurbita pepo subsp. pepo]	[more]

BLAST of PI0003296.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23820.1	1.4e-198	74.48	Pectin lyase-like superfamily protein	[more]
AT5G41870.1	5.4e-179	67.27	Pectin lyase-like superfamily protein	[more]
AT4G33440.1	5.3e-126	53.16	Pectin lyase-like superfamily protein	[more]
AT3G62110.1	9.0e-126	53.57	Pectin lyase-like superfamily protein	[more]
AT3G16850.1	1.2e-117	49.89	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 269..290 e-value: 3700.0 score: 3.1 coord: 325..346 e-value: 130.0 score: 11.4 coord: 208..234 e-value: 300.0 score: 10.2 coord: 296..317 e-value: 1100.0 score: 6.8 coord: 235..267 e-value: 990.0 score: 7.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 25..417 e-value: 1.1E-110 score: 372.4
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 137..418 e-value: 5.5E-41 score: 140.7
None	No IPR available	PANTHER	PTHR31339	PECTIN LYASE-RELATED	coord: 8..442
None	No IPR available	PANTHER	PTHR31339:SF60	POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEIN	coord: 8..442
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 33..434

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
PI0003296	PI0003296	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0003296.1-five_prime_utr	PI0003296.1-five_prime_utr-chr09:1480487..1480720	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0003296.1-exon	PI0003296.1-exon-chr09:1480487..1481050	exon
PI0003296.1-exon	PI0003296.1-exon-chr09:1482543..1482663	exon
PI0003296.1-exon	PI0003296.1-exon-chr09:1482776..1482950	exon
PI0003296.1-exon	PI0003296.1-exon-chr09:1483051..1483121	exon
PI0003296.1-exon	PI0003296.1-exon-chr09:1483280..1484061	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0003296.1-cds	PI0003296.1-cds-chr09:1480721..1481050	CDS
PI0003296.1-cds	PI0003296.1-cds-chr09:1482543..1482663	CDS
PI0003296.1-cds	PI0003296.1-cds-chr09:1482776..1482950	CDS
PI0003296.1-cds	PI0003296.1-cds-chr09:1483051..1483121	CDS
PI0003296.1-cds	PI0003296.1-cds-chr09:1483280..1483920	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0003296.1-three_prime_utr	PI0003296.1-three_prime_utr-chr09:1483921..1484061	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
PI0003296.1	PI0003296.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity