PI0000287.1 (mRNA) Melon (PI 482460) v1

Overview


Name	PI0000287.1
Type	mRNA
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Protein DOUBLE-STRAND BREAK FORMATION
Location	chr12: 16133652 .. 16137344 (+)
Sequence length	1528
RNA-Seq Expression	PI0000287.1
Synteny	PI0000287.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGATTTTGAAGAACAAAGCTTTCCCACCAATTATTAATTGATGTTTTGTTCATACTCTCTCTTTCTTTCGCGCATCAGAAGCCGAAGGTTTGGTTTCGCTATTCACTCTCAACTCTTATCCAACTCATTCAATCGAATTTTCCATCCTTAATCTCCATTTATTATCAATTGTTACATTTTCTCTGATTCTTTACCGCTCAAGATTTCCACAGATTTGATGATTCTACTTTGCGAATTCTGGAATTATTTCCCGCTTCCAAAGACGCGACGTCGTTGATGGATGTTAAATCCAGCTTTAAAGAACTTCTCAGATTTGAATCTCTATCCATCATTCGTGAAACCGCTGAGAAAACTGATGAACAAAAGCTTCTAGTCATCGAATTTCTTGTTCGAGCTTTTGCCCTTGTTGGGGACATTGAGGTGACTATTTATTTGCATAACTCTCTGTGATCGTTCATGCTTTTAGAACGAATTGCCTAGTTTTTTGTATCTGTGATCCTTCAGTTTTTCTTCTGTAATCGTTCATGAATTTCCTAGTTTTGATGTCTGTACTCTATGCTAAATGATGAATTTGTTAGCAGATGTGAATGTTTCTCTAATGTTCTACTTATACTTTCATGCTTCTAGAGTTGCTTAGCTTTGAGATACGAGGCCTTGAATTTTCGGGTACTGAAGTCTTTCAATCAACCATGGCTTCAAGTATCACACGCAGAATGGTTAAACTTCGCTGAGCATTCATTGCATGCTGGCTTTTTTTCAATTTCCATAAAGGTAAGGGGATTTCTCCTATTTATGTAATTGCTTAAGATTTTTCTTTAACACTTTGGTTGAGGATTCTGTCCAATAAAGTTCTACATATTATGTCCTGCATTGGACCACTTATGTATCTACTTATTATGAGAATATGAGATATGAAGTGAGACCAAATGCTTAATAAAAATGCATTTGTCTTTTGGGAAATTAAGAATGCATATGAAGTGGATGTTTGAGGTATGCTGGTGCTATGAATAGTAGTTCAACGAAAAGCCTTGTCTGAAGAATTCTGAAATAAGGAAAGAATAGCTCAGCATAAACAGTGGCTAAGTTTTGCTGGTGATATGCTTGTTGTGGCTAAGTTCATAAGGAAAAGCTTTGTCAGTGATGGTGCTATGCAAATTGTGGGTAGGTTTCAAGAAAGAGAAAGGAAACATCATCAAACTGAACAAATCTAAAGGCTAGTTATTAAGTAGAGAAAACGATGGATAAGGAGAGCCTTTGTCCGAATTTCTGTGAGTTAGTTTTCACATGGTTTGAATTGAAGAATATGCTCATGAAGAACATTGAAGAACATGCATATTTATTAAACGCGAAAAGATTGATTATAGATTTCAAGATTTTGCAGTGAACACTGATATTATCACAAAGTCAAATCAATGGTAAGAAAGAAGAAAAACTCAATGTATGTTGCACAACTTCTACCCATTAGTGCAACAGCAGTAGTGAGTTCATGATAACGGCTTACCTAGACTCGATACACTAATGTGGTAAGATTTCTGTTTGAATGAGCTCTACTGGGACATAATTAGAACAAGAATAATGCCATTGCTGCCAATAATCTTTCTACCATCTGCACAACTAACTCAGTTTACCTCAGTGTATGGTAGGAGAGACCAATTGGGCTACGAAGACAATAAGGTAAGTGGAACATTAAAACAGCTGGGAAATCTGTTGTAGGAGCATTGAGGAATTTATGAAATTGGTAACAGGAAACTGAGGTTTGGTAGAGTAATAGTCCAACGCAATTTAAGACCATAGAAATGGGTATGTCTTGTTAAATCACTACTGAACCAAAAAACTTAAGTTTATGGATTATGGAAAATGTAATTATATCAATACTTTAATACTTCCCCACATTTGTGGGTGTAGAAAGTTGTAGAAAACTCGACAAGTGGAAATCAATATTAATTGGAGTGAAAATGACATTGTATGGTTTGAAACTTTGAACATAGGACCTCCTGTCTTTGTACTATGTGTTAGATGCACAACATCGTTTTTCACTCCAGGCCTATCAACTTTTAGTTCTTGACATTATTTATTTTATCTCGTGTCTTATTCTCTAATTGCAGCTCTAATTTATAGTATTCAAGTACTTTTCTCCTTTAATACAATGAAATATGGTGAAGATTTAATTAGTCTCATTCAATAGGGAGAACTTATTTACACTTTTCTTTGGTAGGCATATGAGCAAGCACTGTCAAGCCTTCAGCAGAGTGATACTGCGAACTACACATCACATGGTTCCTTTAAAAGCACAGAAGTTATCGAGAAGATAAATAGACTCAAAGATCATGCTCTGAATTTAGCTGGTTCCCATTCTGGTAGGGATCTTAGTCGAGTTAAAAGATTTGATGATCAATTCAATCACTAAATTAGCTGTATGCTTTGGGTTCTAATTTGATTAAGAACTAACTGTTTCACTTTTTATCGAAGGCATTGCATCTGTTGATGATTGACTTTCATGGATAAGATTTTTGACTTCTTCAAACTTCGTGCTTTTTGACTCCCTTGTTGTGTCTTAACCTAACATCCAGTGCTTCCATCATAATATTATTTTCAAACAACTTGTTATTCATAAGGAGAAAAAAGAAAATAACCAAATGCCTTCTATTTCTAATACATAGTTTTAATAACTAATAGTTTGGAATGTACACAATCTTTTAGTCCAAATACCATCTGACTCCTTGATCATTGTGTCTTATTAAACCAGTTCAAGCTCTCACATCTGATTATTTGAAAAAGAAAGTAACTGAAAGGAACAGAAAGATTTCTTCATCCTGCACAAAAAAGTTTACAGCGAGCACTCTATTCAGAAATGGTATCAGAAACTACAATGCAAGAAAGCTGCATGAATATCGAAGTTTTGGAGGGGTTAACCAGAAAGTCGCACAAAATCCAGTTTTGTGATCAGACCTACATTTAGTCTCTCTTCATACCCGTGTTACTAGATAAATGTTCAGGTTAGTTTTACACGCATGAGTAACATGAGTAACTTTTCCTCTTATCAGTGTAATAGTTCTTTACACGCATGGTGAAGATTTAATTAGTCTCAGTGTAATAGTTCTTTATAATTTTACAATGACAGTAACTAGCGGATGTGAACTTTGATGGCATAGAACCGTAATTCACTTAACTTGCGGTTTAGGAGTAGGGTTAAAGTTGATGCTATGCATTTGTTCTTTAATCAATACCAAGAGGGGGCTAGACTTTATTATCGAGTGATGTAAATGATGTTCGGTGAGACTTGAGTTAAGTAGGAAATAGATGAGTAGTTTGGCGAGAGAATTGAGTCTAAATAATATTTCTTCTCTCAATAGGTTGAGATAACTAGAAGAGGCTCTATCGAATTCCCCGTGACTGTTTCTTCCCACATCTTTTTTTGAAAGAGAAGTTTAAAAGCTATTGCGCTAGATAATTTATTCTTGCTTATATCATAGACTGGTTAATGCTATTTAGCTTAGAAGTATGGACAATTTTGTTTGACTAGTGTGCCATGTCCGGATGATGTGTGTGCATTTGCAGGTATATGTATTATTAGACTATAGTAAAAGCAATAGAAGTACTTTTGGTTTATCAAAGTGTGATTACCAGACGATTTCTCCTGCGCTAGATAATTCTTTTCATTTTATTTTATTTATAAATTTGATTGGGATTGAG

mRNA sequence

GGATTTTGAAGAACAAAGCTTTCCCACCAATTATTAATTGATGTTTTGTTCATACTCTCTCTTTCTTTCGCGCATCAGAAGCCGAAGATTTGATGATTCTACTTTGCGAATTCTGGAATTATTTCCCGCTTCCAAAGACGCGACGTCGTTGATGGATGTTAAATCCAGCTTTAAAGAACTTCTCAGATTTGAATCTCTATCCATCATTCGTGAAACCGCTGAGAAAACTGATGAACAAAAGCTTCTAGTCATCGAATTTCTTGTTCGAGCTTTTGCCCTTGTTGGGGACATTGAGAGTTGCTTAGCTTTGAGATACGAGGCCTTGAATTTTCGGGTACTGAAGTCTTTCAATCAACCATGGCTTCAAGTATCACACGCAGAATGGTTAAACTTCGCTGAGCATTCATTGCATGCTGGCTTTTTTTCAATTTCCATAAAGGCATATGAGCAAGCACTGTCAAGCCTTCAGCAGAGTGATACTGCGAACTACACATCACATGGTTCCTTTAAAAGCACAGAAGTTATCGAGAAGATAAATAGACTCAAAGATCATGCTCTGAATTTAGCTGGTTCCCATTCTGTTCAAGCTCTCACATCTGATTATTTGAAAAAGAAAGTAACTGAAAGGAACAGAAAGATTTCTTCATCCTGCACAAAAAAGTTTACAGCGAGCACTCTATTCAGAAATGGTATCAGAAACTACAATGCAAGAAAGCTGCATGAATATCGAAGTTTTGGAGGGGTTAACCAGAAAGTCGCACAAAATCCAGTTTTGTGATCAGACCTACATTTAGTCTCTCTTCATACCCGTGTTACTAGATAAATGTTCAGGTTAGTTTTACACGCATGAGTAACATGAGTAACTTTTCCTCTTATCAGTGTAATAGTTCTTTACACGCATGGTGAAGATTTAATTAGTCTCAGTGTAATAGTTCTTTATAATTTTACAATGACAGTAACTAGCGGATGTGAACTTTGATGGCATAGAACCGTAATTCACTTAACTTGCGGTTTAGGAGTAGGGTTAAAGTTGATGCTATGCATTTGTTCTTTAATCAATACCAAGAGGGGGCTAGACTTTATTATCGAGTGATGTAAATGATGTTCGGTGAGACTTGAGTTAAGTAGGAAATAGATGAGTAGTTTGGCGAGAGAATTGAGTCTAAATAATATTTCTTCTCTCAATAGGTTGAGATAACTAGAAGAGGCTCTATCGAATTCCCCGTGACTGTTTCTTCCCACATCTTTTTTTGAAAGAGAAGTTTAAAAGCTATTGCGCTAGATAATTTATTCTTGCTTATATCATAGACTGGTTAATGCTATTTAGCTTAGAAGTATGGACAATTTTGTTTGACTAGTGTGCCATGTCCGGATGATGTGTGTGCATTTGCAGGTATATGTATTATTAGACTATAGTAAAAGCAATAGAAGTACTTTTGGTTTATCAAAGTGTGATTACCAGACGATTTCTCCTGCGCTAGATAATTCTTTTCATTTTATTTTATTTATAAATTTGATTGGGATTGAG

Coding sequence (CDS)

ATGTTTTGTTCATACTCTCTCTTTCTTTCGCGCATCAGAAGCCGAAGATTTGATGATTCTACTTTGCGAATTCTGGAATTATTTCCCGCTTCCAAAGACGCGACGTCGTTGATGGATGTTAAATCCAGCTTTAAAGAACTTCTCAGATTTGAATCTCTATCCATCATTCGTGAAACCGCTGAGAAAACTGATGAACAAAAGCTTCTAGTCATCGAATTTCTTGTTCGAGCTTTTGCCCTTGTTGGGGACATTGAGAGTTGCTTAGCTTTGAGATACGAGGCCTTGAATTTTCGGGTACTGAAGTCTTTCAATCAACCATGGCTTCAAGTATCACACGCAGAATGGTTAAACTTCGCTGAGCATTCATTGCATGCTGGCTTTTTTTCAATTTCCATAAAGGCATATGAGCAAGCACTGTCAAGCCTTCAGCAGAGTGATACTGCGAACTACACATCACATGGTTCCTTTAAAAGCACAGAAGTTATCGAGAAGATAAATAGACTCAAAGATCATGCTCTGAATTTAGCTGGTTCCCATTCTGTTCAAGCTCTCACATCTGATTATTTGAAAAAGAAAGTAACTGAAAGGAACAGAAAGATTTCTTCATCCTGCACAAAAAAGTTTACAGCGAGCACTCTATTCAGAAATGGTATCAGAAACTACAATGCAAGAAAGCTGCATGAATATCGAAGTTTTGGAGGGGTTAACCAGAAAGTCGCACAAAATCCAGTTTTGTGA

Protein sequence

MFCSYSLFLSRIRSRRFDDSTLRILELFPASKDATSLMDVKSSFKELLRFESLSIIRETAEKTDEQKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAEHSLHAGFFSISIKAYEQALSSLQQSDTANYTSHGSFKSTEVIEKINRLKDHALNLAGSHSVQALTSDYLKKKVTERNRKISSSCTKKFTASTLFRNGIRNYNARKLHEYRSFGGVNQKVAQNPVL

Homology

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BLAST of PI0000287.1 vs. ExPASy Swiss-Prot
Match: Q8RX33 (Protein DOUBLE-STRAND BREAK FORMATION OS=Arabidopsis thaliana OX=3702 GN=DFO PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 4.8e-33
Identity = 80/174 (45.98%), Postives = 112/174 (64.37%), Query Frame = 0


Query: 7   LFLSRIRSRRFDDSTLRILELFPASKDATSLMDVKSSFKELLRFESLSIIRETAEKTDEQ 66
           LF++R++ RRFD+ +LRILEL   + +  S ++V+S  ++ +R ES+ I  E   ++   
Sbjct: 38  LFINRVQDRRFDEESLRILELSLVAMNVKSFLEVRSRLRDFMRSESVVIFGELTGESMVA 97

Query: 67  KLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAEHSLHAG 126
           KL V+EF  RAFAL+GD+ESCLA+RYEALN R LKS +  WL VSH+EW  FA  S+  G
Sbjct: 98  KLSVLEFFARAFALLGDMESCLAMRYEALNLRQLKSPSCLWLGVSHSEWTKFAVQSMENG 157

Query: 127 FFSISIKAYEQALSSLQQSDTANYTSHGSFKSTEVIEKINRLKDHALNLAGSHS 181
           F SI+ KA E AL SL++       S  +    +  EK+ RL+D A +L  SHS
Sbjct: 158 FPSIAGKASENALLSLKKDSLIEPKSEDNSDILDAAEKVRRLRDSAASLTSSHS 211

[Close]

BLAST of PI0000287.1 vs. ExPASy TrEMBL
Match: A0A1S3CL48 (uncharacterized protein LOC103502216 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502216 PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 2.8e-124
Identity = 235/245 (95.92%), Postives = 241/245 (98.37%), Query Frame = 0


Query: 1   MFCSYSLFLSRIRSRRFDDSTLRILELFPASKDATSLMDVKSSFKELLRFESLSIIRETA 60
           MFCSYSLFLSR+RSRRFDDSTLRILELFPASKDATSL DVKSSF ELLRFESLSIIRETA
Sbjct: 1   MFCSYSLFLSRLRSRRFDDSTLRILELFPASKDATSLADVKSSFSELLRFESLSIIRETA 60

Query: 61  EKTDEQKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAE 120
           EKTD+QKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAE
Sbjct: 61  EKTDDQKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAE 120

Query: 121 HSLHAGFFSISIKAYEQALSSLQQSDTANYTSHGSFKSTEVIEKINRLKDHALNLAGSHS 180
           HSLHAGFFSI+IKAYEQALSSLQQSDTANYTSHGSFK TEVIEKINRLKDHALNL+GSHS
Sbjct: 121 HSLHAGFFSIAIKAYEQALSSLQQSDTANYTSHGSFKHTEVIEKINRLKDHALNLSGSHS 180

Query: 181 VQALTSDYLKKKVTERNRKISSSCTKKFTASTLFRNGIRNYNARKLHEYRSFGGVNQKVA 240
           VQALTSDYLKKKVTER+RKISSSCT+KFTASTLFRNGIRNYNARKLHEYRS GGVNQKVA
Sbjct: 181 VQALTSDYLKKKVTERSRKISSSCTRKFTASTLFRNGIRNYNARKLHEYRSIGGVNQKVA 240

Query: 241 QNPVL 246
           QNPVL
Sbjct: 241 QNPVL 245

[Close]

BLAST of PI0000287.1 vs. ExPASy TrEMBL
Match: A0A0A0LSV1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171020 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 3.9e-118
Identity = 225/237 (94.94%), Postives = 231/237 (97.47%), Query Frame = 0


Query: 1   MFCSYSLFLSRIRSRRFDDSTLRILELFPASKDATSLMDVKSSFKELLRFESLSIIRETA 60
           MFCSYSLFLSR+RSRRFDDSTLRILE FPASKDATSLMDV SSFKE+LRFESLSIIRET+
Sbjct: 1   MFCSYSLFLSRLRSRRFDDSTLRILESFPASKDATSLMDVNSSFKEVLRFESLSIIRETS 60

Query: 61  EKTDEQKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAE 120
           EKTD+ KLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAE
Sbjct: 61  EKTDDHKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAE 120

Query: 121 HSLHAGFFSISIKAYEQALSSLQQSDTANYTSHGSFKSTEVIEKINRLKDHALNLAGSHS 180
           HSLHAGFFSISIKAYEQALSSLQQSDTANYTSHGSFK TEV+EKINRLKDHALNLAGSHS
Sbjct: 121 HSLHAGFFSISIKAYEQALSSLQQSDTANYTSHGSFKRTEVMEKINRLKDHALNLAGSHS 180

Query: 181 VQALTSDYLKKKVTERNRKISSSCTKKFTASTLFRNGIRNYNARKLHEYRSFGGVNQ 238
           VQALTSDYLKKKVTERNRKISSSCT+KFTASTLF NGIRNYNARKLHEYRSF GVNQ
Sbjct: 181 VQALTSDYLKKKVTERNRKISSSCTRKFTASTLFINGIRNYNARKLHEYRSFEGVNQ 237

[Close]

BLAST of PI0000287.1 vs. ExPASy TrEMBL
Match: A0A6J1I136 (uncharacterized protein LOC111469552 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469552 PE=4 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 5.1e-102
Identity = 201/240 (83.75%), Postives = 213/240 (88.75%), Query Frame = 0


Query: 5   YSLFLSRIRSRRFDDSTLRILELFPASKDATSLMDVKSSFKELLRFESLSIIRETAEKTD 64
           YSLF SR+RSRRFDDSTLRILE F ASKD    MDVKS  KELLRFESLSIIRET EKTD
Sbjct: 9   YSLFCSRLRSRRFDDSTLRILEFFSASKDTMYFMDVKSGVKELLRFESLSIIRETVEKTD 68

Query: 65  EQKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAEHSLH 124
           +QKLLVIEFLVRAFALVGDIESCLALRYEALNFR LKSFNQP LQVSHAEWLNFAEHSL+
Sbjct: 69  DQKLLVIEFLVRAFALVGDIESCLALRYEALNFRELKSFNQPRLQVSHAEWLNFAEHSLN 128

Query: 125 AGFFSISIKAYEQALSSLQQSDTANYTSHGSFKSTEVIEKINRLKDHALNLAGSHSVQAL 184
           AGFFSI+IKAYEQALSSLQQSDTANYTSHGS K  EVIEKI RLKDHAL  AGSHSVQAL
Sbjct: 129 AGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKRAEVIEKIKRLKDHALKSAGSHSVQAL 188

Query: 185 TSDYLKKKVTERNRKISSSCTKKFTASTLFRNGIRNYNARKLHEYRSFGGVNQKVAQNPV 244
           TS+YLKKKVTERNRKISSSCT+KFTASTLFRNGIRN+NA+KLHEY++  G+       P+
Sbjct: 189 TSEYLKKKVTERNRKISSSCTRKFTASTLFRNGIRNHNAKKLHEYQALEGLTSNRLLGPL 248

[Close]

BLAST of PI0000287.1 vs. ExPASy TrEMBL
Match: A0A6J1I645 (uncharacterized protein LOC111469552 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469552 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.1e-101
Identity = 200/234 (85.47%), Postives = 212/234 (90.60%), Query Frame = 0


Query: 5   YSLFLSRIRSRRFDDSTLRILELFPASKDATSLMDVKSSFKELLRFESLSIIRETAEKTD 64
           YSLF SR+RSRRFDDSTLRILE F ASKD    MDVKS  KELLRFESLSIIRET EKTD
Sbjct: 9   YSLFCSRLRSRRFDDSTLRILEFFSASKDTMYFMDVKSGVKELLRFESLSIIRETVEKTD 68

Query: 65  EQKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAEHSLH 124
           +QKLLVIEFLVRAFALVGDIESCLALRYEALNFR LKSFNQP LQVSHAEWLNFAEHSL+
Sbjct: 69  DQKLLVIEFLVRAFALVGDIESCLALRYEALNFRELKSFNQPRLQVSHAEWLNFAEHSLN 128

Query: 125 AGFFSISIKAYEQALSSLQQSDTANYTSHGSFKSTEVIEKINRLKDHALNLAGSHSVQAL 184
           AGFFSI+IKAYEQALSSLQQSDTANYTSHGS K  EVIEKI RLKDHAL  AGSHSVQAL
Sbjct: 129 AGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKRAEVIEKIKRLKDHALKSAGSHSVQAL 188

Query: 185 TSDYLKKKVTERNRKISSSCTKKFTASTLFRNGIRNYNARKLHEYRSFGGVNQK 239
           TS+YLKKKVTERNRKISSSCT+KFTASTLFRNGIRN+NA+KLHEY++  G+  +
Sbjct: 189 TSEYLKKKVTERNRKISSSCTRKFTASTLFRNGIRNHNAKKLHEYQALEGLTSE 242

[Close]

BLAST of PI0000287.1 vs. ExPASy TrEMBL
Match: A0A6J1HPP0 (uncharacterized protein LOC111464906 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464906 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 9.6e-101
Identity = 198/234 (84.62%), Postives = 212/234 (90.60%), Query Frame = 0


Query: 5   YSLFLSRIRSRRFDDSTLRILELFPASKDATSLMDVKSSFKELLRFESLSIIRETAEKTD 64
           YSLF SR+RSRR DDSTLRILE F ASKD  SLMDVKS  KELL FESLSIIRET EKTD
Sbjct: 9   YSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSIIRETVEKTD 68

Query: 65  EQKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAEHSLH 124
           +QKLLVIEFLVRAFALVGDIESCLALRYEALNFR LKSFNQP LQVSHAEWLNFAEHSL+
Sbjct: 69  DQKLLVIEFLVRAFALVGDIESCLALRYEALNFRELKSFNQPRLQVSHAEWLNFAEHSLN 128

Query: 125 AGFFSISIKAYEQALSSLQQSDTANYTSHGSFKSTEVIEKINRLKDHALNLAGSHSVQAL 184
           AGFFSI+IKAYEQALSSLQQSDTANYTSHGS K  EVIEKI RLKDHAL  AGSHSVQAL
Sbjct: 129 AGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKDHALKSAGSHSVQAL 188

Query: 185 TSDYLKKKVTERNRKISSSCTKKFTASTLFRNGIRNYNARKLHEYRSFGGVNQK 239
           TS+YLKK+VTERNRKISSSCT+KFTASTLFRNGIRN+NA++LHEY++  G+  +
Sbjct: 189 TSEYLKKRVTERNRKISSSCTRKFTASTLFRNGIRNHNAKRLHEYQALEGLTSE 242

[Close]

BLAST of PI0000287.1 vs. NCBI nr
Match: XP_008464290.1 (PREDICTED: uncharacterized protein LOC103502216 isoform X1 [Cucumis melo] >XP_008464291.1 PREDICTED: uncharacterized protein LOC103502216 isoform X1 [Cucumis melo] >XP_008464292.1 PREDICTED: uncharacterized protein LOC103502216 isoform X1 [Cucumis melo] >XP_016903176.1 PREDICTED: uncharacterized protein LOC103502216 isoform X1 [Cucumis melo] >XP_016903177.1 PREDICTED: uncharacterized protein LOC103502216 isoform X1 [Cucumis melo] >XP_016903178.1 PREDICTED: uncharacterized protein LOC103502216 isoform X1 [Cucumis melo] >XP_016903179.1 PREDICTED: uncharacterized protein LOC103502216 isoform X1 [Cucumis melo])

HSP 1 Score: 454.5 bits (1168), Expect = 5.7e-124
Identity = 235/245 (95.92%), Postives = 241/245 (98.37%), Query Frame = 0


Query: 1   MFCSYSLFLSRIRSRRFDDSTLRILELFPASKDATSLMDVKSSFKELLRFESLSIIRETA 60
           MFCSYSLFLSR+RSRRFDDSTLRILELFPASKDATSL DVKSSF ELLRFESLSIIRETA
Sbjct: 1   MFCSYSLFLSRLRSRRFDDSTLRILELFPASKDATSLADVKSSFSELLRFESLSIIRETA 60

Query: 61  EKTDEQKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAE 120
           EKTD+QKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAE
Sbjct: 61  EKTDDQKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAE 120

Query: 121 HSLHAGFFSISIKAYEQALSSLQQSDTANYTSHGSFKSTEVIEKINRLKDHALNLAGSHS 180
           HSLHAGFFSI+IKAYEQALSSLQQSDTANYTSHGSFK TEVIEKINRLKDHALNL+GSHS
Sbjct: 121 HSLHAGFFSIAIKAYEQALSSLQQSDTANYTSHGSFKHTEVIEKINRLKDHALNLSGSHS 180

Query: 181 VQALTSDYLKKKVTERNRKISSSCTKKFTASTLFRNGIRNYNARKLHEYRSFGGVNQKVA 240
           VQALTSDYLKKKVTER+RKISSSCT+KFTASTLFRNGIRNYNARKLHEYRS GGVNQKVA
Sbjct: 181 VQALTSDYLKKKVTERSRKISSSCTRKFTASTLFRNGIRNYNARKLHEYRSIGGVNQKVA 240

Query: 241 QNPVL 246
           QNPVL
Sbjct: 241 QNPVL 245

[Close]

BLAST of PI0000287.1 vs. NCBI nr
Match: XP_004139510.2 (protein DOUBLE-STRAND BREAK FORMATION isoform X1 [Cucumis sativus] >KGN64980.1 hypothetical protein Csa_022826 [Cucumis sativus])

HSP 1 Score: 434.1 bits (1115), Expect = 8.0e-118
Identity = 225/237 (94.94%), Postives = 231/237 (97.47%), Query Frame = 0


Query: 1   MFCSYSLFLSRIRSRRFDDSTLRILELFPASKDATSLMDVKSSFKELLRFESLSIIRETA 60
           MFCSYSLFLSR+RSRRFDDSTLRILE FPASKDATSLMDV SSFKE+LRFESLSIIRET+
Sbjct: 1   MFCSYSLFLSRLRSRRFDDSTLRILESFPASKDATSLMDVNSSFKEVLRFESLSIIRETS 60

Query: 61  EKTDEQKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAE 120
           EKTD+ KLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAE
Sbjct: 61  EKTDDHKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAE 120

Query: 121 HSLHAGFFSISIKAYEQALSSLQQSDTANYTSHGSFKSTEVIEKINRLKDHALNLAGSHS 180
           HSLHAGFFSISIKAYEQALSSLQQSDTANYTSHGSFK TEV+EKINRLKDHALNLAGSHS
Sbjct: 121 HSLHAGFFSISIKAYEQALSSLQQSDTANYTSHGSFKRTEVMEKINRLKDHALNLAGSHS 180

Query: 181 VQALTSDYLKKKVTERNRKISSSCTKKFTASTLFRNGIRNYNARKLHEYRSFGGVNQ 238
           VQALTSDYLKKKVTERNRKISSSCT+KFTASTLF NGIRNYNARKLHEYRSF GVNQ
Sbjct: 181 VQALTSDYLKKKVTERNRKISSSCTRKFTASTLFINGIRNYNARKLHEYRSFEGVNQ 237

[Close]

BLAST of PI0000287.1 vs. NCBI nr
Match: XP_038895344.1 (protein DOUBLE-STRAND BREAK FORMATION isoform X1 [Benincasa hispida])

HSP 1 Score: 380.6 bits (976), Expect = 1.0e-101
Identity = 202/242 (83.47%), Postives = 214/242 (88.43%), Query Frame = 0


Query: 1   MFCS----YSLFLSRIRSRRFDDSTLRILELFPASKDATSLMDVKSSFKELLRFESLSII 60
           M CS    YSLF SR+RSRRFDDSTLRILE FPASKDA SLMDVKS  KE LRFESLSII
Sbjct: 1   MSCSAAEQYSLFRSRLRSRRFDDSTLRILEFFPASKDAMSLMDVKSDLKEFLRFESLSII 60

Query: 61  RETAEKTDEQKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWL 120
           RETAEKTD+QKLLVIEFLVRAFALVGDIESCLALRYEALNFR+LKSFNQPWLQVSHAEWL
Sbjct: 61  RETAEKTDDQKLLVIEFLVRAFALVGDIESCLALRYEALNFRLLKSFNQPWLQVSHAEWL 120

Query: 121 NFAEHSLHAGFFSISIKAYEQALSSLQQSDTANYTSHGSFKSTEVIEKINRLKDHALNLA 180
           NFAEHSL AGFFSI+IKAYEQALSSLQQ+DT NYTSHGS K  EVIEKI RLKDHAL  A
Sbjct: 121 NFAEHSLRAGFFSIAIKAYEQALSSLQQNDTENYTSHGSLKRIEVIEKIKRLKDHALRSA 180

Query: 181 GSHSVQALTSDYLKKKVTERNRKISSSCTKKFTASTLFRNGIRNYNARKLHEYRSFGGVN 239
           GSHSVQALTS+YL KKVTERN KISSSCT+K TASTLFRNG RN+NA+KLHEY+   G+ 
Sbjct: 181 GSHSVQALTSEYLNKKVTERNIKISSSCTRKSTASTLFRNGFRNHNAKKLHEYQVLEGLT 240

[Close]

BLAST of PI0000287.1 vs. NCBI nr
Match: XP_022970621.1 (uncharacterized protein LOC111469552 isoform X2 [Cucurbita maxima])

HSP 1 Score: 380.6 bits (976), Expect = 1.0e-101
Identity = 201/240 (83.75%), Postives = 213/240 (88.75%), Query Frame = 0


Query: 5   YSLFLSRIRSRRFDDSTLRILELFPASKDATSLMDVKSSFKELLRFESLSIIRETAEKTD 64
           YSLF SR+RSRRFDDSTLRILE F ASKD    MDVKS  KELLRFESLSIIRET EKTD
Sbjct: 9   YSLFCSRLRSRRFDDSTLRILEFFSASKDTMYFMDVKSGVKELLRFESLSIIRETVEKTD 68

Query: 65  EQKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAEHSLH 124
           +QKLLVIEFLVRAFALVGDIESCLALRYEALNFR LKSFNQP LQVSHAEWLNFAEHSL+
Sbjct: 69  DQKLLVIEFLVRAFALVGDIESCLALRYEALNFRELKSFNQPRLQVSHAEWLNFAEHSLN 128

Query: 125 AGFFSISIKAYEQALSSLQQSDTANYTSHGSFKSTEVIEKINRLKDHALNLAGSHSVQAL 184
           AGFFSI+IKAYEQALSSLQQSDTANYTSHGS K  EVIEKI RLKDHAL  AGSHSVQAL
Sbjct: 129 AGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKRAEVIEKIKRLKDHALKSAGSHSVQAL 188

Query: 185 TSDYLKKKVTERNRKISSSCTKKFTASTLFRNGIRNYNARKLHEYRSFGGVNQKVAQNPV 244
           TS+YLKKKVTERNRKISSSCT+KFTASTLFRNGIRN+NA+KLHEY++  G+       P+
Sbjct: 189 TSEYLKKKVTERNRKISSSCTRKFTASTLFRNGIRNHNAKKLHEYQALEGLTSNRLLGPL 248

[Close]

BLAST of PI0000287.1 vs. NCBI nr
Match: XP_023520164.1 (uncharacterized protein LOC111783465 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 379.8 bits (974), Expect = 1.8e-101
Identity = 199/231 (86.15%), Postives = 213/231 (92.21%), Query Frame = 0


Query: 5   YSLFLSRIRSRRFDDSTLRILELFPASKDATSLMDVKSSFKELLRFESLSIIRETAEKTD 64
           YSLF SR+RSRRFDDSTLRILE F ASKD  SLMDVKS  KELLRFESLSIIRET +KTD
Sbjct: 9   YSLFCSRLRSRRFDDSTLRILEFFSASKDTMSLMDVKSGVKELLRFESLSIIRETVDKTD 68

Query: 65  EQKLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAEHSLH 124
           +QKLLVIEFLVRAFALVGDIESCLALRYEALNFR LKSFNQP LQVSHAEWLNFAEHSL+
Sbjct: 69  DQKLLVIEFLVRAFALVGDIESCLALRYEALNFRELKSFNQPRLQVSHAEWLNFAEHSLN 128

Query: 125 AGFFSISIKAYEQALSSLQQSDTANYTSHGSFKSTEVIEKINRLKDHALNLAGSHSVQAL 184
           AGFFSI++KAYEQALSSLQQSDTANYTSHGS K  EVIEKI RLKDH+L  AGSHSVQAL
Sbjct: 129 AGFFSIAMKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKDHSLKSAGSHSVQAL 188

Query: 185 TSDYLKKKVTERNRKISSSCTKKFTASTLFRNGIRNYNARKLHEYRSFGGV 236
           TS+YLKKKVTERNRKISSSCT+KFTASTLFRNGIRN+NA+KLHEY++  G+
Sbjct: 189 TSEYLKKKVTERNRKISSSCTRKFTASTLFRNGIRNHNAKKLHEYQALEGL 239

[Close]

BLAST of PI0000287.1 vs. TAIR 10
Match: AT1G07060.1 (unknown protein; Has 30 Blast hits to 30 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 30; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 142.9 bits (359), Expect = 3.4e-34
Identity = 80/174 (45.98%), Postives = 112/174 (64.37%), Query Frame = 0


Query: 7   LFLSRIRSRRFDDSTLRILELFPASKDATSLMDVKSSFKELLRFESLSIIRETAEKTDEQ 66
           LF++R++ RRFD+ +LRILEL   + +  S ++V+S  ++ +R ES+ I  E   ++   
Sbjct: 38  LFINRVQDRRFDEESLRILELSLVAMNVKSFLEVRSRLRDFMRSESVVIFGELTGESMVA 97

Query: 67  KLLVIEFLVRAFALVGDIESCLALRYEALNFRVLKSFNQPWLQVSHAEWLNFAEHSLHAG 126
           KL V+EF  RAFAL+GD+ESCLA+RYEALN R LKS +  WL VSH+EW  FA  S+  G
Sbjct: 98  KLSVLEFFARAFALLGDMESCLAMRYEALNLRQLKSPSCLWLGVSHSEWTKFAVQSMENG 157

Query: 127 FFSISIKAYEQALSSLQQSDTANYTSHGSFKSTEVIEKINRLKDHALNLAGSHS 181
           F SI+ KA E AL SL++       S  +    +  EK+ RL+D A +L  SHS
Sbjct: 158 FPSIAGKASENALLSLKKDSLIEPKSEDNSDILDAAEKVRRLRDSAASLTSSHS 211

The following BLAST results are available for this feature:

BLAST of PI0000287.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RX33	4.8e-33	45.98	Protein DOUBLE-STRAND BREAK FORMATION OS=Arabidopsis thaliana OX=3702 GN=DFO PE=...	[more]

BLAST of PI0000287.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CL48	2.8e-124	95.92	uncharacterized protein LOC103502216 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0LSV1	3.9e-118	94.94	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171020 PE=4 SV=1	[more]
A0A6J1I136	5.1e-102	83.75	uncharacterized protein LOC111469552 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1I645	1.1e-101	85.47	uncharacterized protein LOC111469552 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1HPP0	9.6e-101	84.62	uncharacterized protein LOC111464906 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of PI0000287.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008464290.1	5.7e-124	95.92	PREDICTED: uncharacterized protein LOC103502216 isoform X1 [Cucumis melo] >XP_00...	[more]
XP_004139510.2	8.0e-118	94.94	protein DOUBLE-STRAND BREAK FORMATION isoform X1 [Cucumis sativus] >KGN64980.1 h...	[more]
XP_038895344.1	1.0e-101	83.47	protein DOUBLE-STRAND BREAK FORMATION isoform X1 [Benincasa hispida]	[more]
XP_022970621.1	1.0e-101	83.75	uncharacterized protein LOC111469552 isoform X2 [Cucurbita maxima]	[more]
XP_023520164.1	1.8e-101	86.15	uncharacterized protein LOC111783465 isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of PI0000287.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G07060.1	3.4e-34	45.98	unknown protein; Has 30 Blast hits to 30 proteins in 10 species: Archae - 0; Bac...	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044969	Protein DOUBLE-STRAND BREAK FORMATION	PANTHER	PTHR37176	F10K1.23	coord: 4..238

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
PI0000287	PI0000287	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0000287.1-five_prime_utr	PI0000287.1-five_prime_utr-chr12:16133652..16133691	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0000287.1-exon	PI0000287.1-exon-chr12:16133652..16133738	exon
PI0000287.1-exon	PI0000287.1-exon-chr12:16133864..16134071	exon
PI0000287.1-exon	PI0000287.1-exon-chr12:16134279..16134422	exon
PI0000287.1-exon	PI0000287.1-exon-chr12:16135867..16136008	exon
PI0000287.1-exon	PI0000287.1-exon-chr12:16136398..16137344	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0000287.1-cds	PI0000287.1-cds-chr12:16133692..16133738	CDS
PI0000287.1-cds	PI0000287.1-cds-chr12:16133864..16134071	CDS
PI0000287.1-cds	PI0000287.1-cds-chr12:16134279..16134422	CDS
PI0000287.1-cds	PI0000287.1-cds-chr12:16135867..16136008	CDS
PI0000287.1-cds	PI0000287.1-cds-chr12:16136398..16136594	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0000287.1-three_prime_utr	PI0000287.1-three_prime_utr-chr12:16136595..16137344	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
PI0000287.1	PI0000287.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042138	meiotic DNA double-strand break formation