Moc10g01010.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g01010.1
TypemRNA
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPyruvate kinase
Locationchr10: 621624 .. 626706 (-)
Sequence length1722
RNA-Seq ExpressionMoc10g01010.1
SyntenyMoc10g01010.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCAGGTGGTGGCTACAAGGTCGATTTGCAGCACATTCCTGTGCCCTAGCTCGGGATCTGTGCAGGACCGGGCGGACAAGGCTCGGTCTTTTGGTTTTGGCTCGAAAGTGCTGGCGCACGAGAAGAAGAGTTGGAACTTTGTTTCCAGGAGAAGTCTAGTCACAGCGAAAAGAGCTGCTCAAGCTGCAGTTGTTCCAGTATCACCTGAGGATGTTCAGAAGGTTTGTGATCTTTTGAGCTGTTAACTTTTTTAGGAAAATGCTTTTATTTGCTTGTTACGAAAAGATTGAGAAAACAAGGGGAGCTGGAGTCATGGAATTCGAATATCTGTAAGAATTAAGGAGCTGTATGACAGGCACGTAGCATTCAGGGAGCTAAATGGTGTATTTTATTATATTGCTTCAAGCTGTACGGATACATAATTGGGTATAATTCCTGCATTTGGATGCGTAGAGTTTTGTACGGCTGGAAATTCAGAATGCATATTGCTGTTGCTGAGAAAGTAAATCATCAGAAAATGAGATTTTCCCCTTATATATAGTAATATAACATGAATTAACATATATATATGAATGGTTGCCGAGAATCCTCTTCTAGTTTTCTTATTTTGTTTACCAATGGAAATGGATACTGTGTAGTCTTCATTGGCTGTAAATGCTAGTGATTAGTGAATTTAAAATTTCTCGTCTTAATATGTTTCTAAATCTTGCTTATCAGGCAGATGAGCAATTTGAACATTTACGAGCAGTTCAACAACTTGGTGATACACCGGTTGGAATGTGGTCCAAACCAACAGTTAGACGTAAGACAAAGATTGTGTGCACGATTGGTCCTTCGACTAACACGAAGGAAATGATTTGGAAACTGGCCGAGGCTGGAATGAATGTTGCTCGATTGAATATGTCTCATGGAGACCATGCATCTCATCAGAAAGTTATAGATTTAGTCAGAGAATACAATGCGCAATCACAAGACAACTGCATTGCGATCATGCTCGACACCAAGGCAAGTTCATTTTCATATTTTCATATGTTCTGTACTTTCATTAACCTAGCAACAAATGTTTTTCAATGTAACAAGTTCAATTTTCCTGGAATTTACAAATATTGTATTATCCATCTTTACTCTGCCAGAGGATAAGGATGCACCACCAAGGTTAAAAGAATCTTCTAAAAACAAGTCTACAGTTTCAACTTTACAATGCATTTTGTTTGTTGACTGCTGAAATGCTAATGTCATACATACCCTTTTGTTTTATTCACATTTTTGTCTCTTTTAAGCCTTGATTTTTTCCTTGTGCTGAATATTGACAAACGGTAGACCGATTCATTATGTAACCAATTACGTTTTCTAATTTTTTTTTTGTTTTTTTGGTAAATTTGGTATAACCATTACGTCATTCAGGGTTTTTCTTTTTAATCATGTATTTCATTTTGCAGGGTCCTGAGGTTAGGAGTGGTGACCTACCACAACCAATATTGTTAGAGACTGGACAGGAATTCACATTTTCCATACGGAGAGGTGTTAGTACTCCAGATTCTGTCAGCGTAAACTATGACGATTTTGTTAATGATGTAGAAGTTGGGGACATGCTTCTTGTTGATGGTATGCCGCATTCTTTTATTTGATTCTCCTTTCTGTGTGTATATATCCTAATGATTAAAATCTTAGTTTACATTCCATGCGGTGAAAAGTCTTGGCATTGTAGCATATGATTTTTGACGTTGTAGTATTATCTTGATATACTTATGTGGCTTCATTTTTCTTTTGGGGACATGTTAATTTCATTTAATGACTGTATAAAGATACCCTTATATTTGTATTTTAATGCTAATTTCACTTTTTTTTTTCTTTCTCATACGTTGTAAACTATCCTTACTATTTTAAATGAAATTTTCATTTTACATCTTAAGATTATTACTACTTGAAATTTATATCAAGAAGAGATAAAGCTTTTTTGTCATCATCTCATGCATTGCAGCAACATTGACTCTTGAAATCTTGTTATAGGTGGAATGATGTCATTGATGGTGAAGTCTAAGACAGAGGATTCGGTGAAGTGTGAAGTTGTTGATGGAGGAGAGCTCAAGTCCAGACGACATTTAAATGTTCGAGGGAAAAGTGCAACACTGCCTTCCATTACTGGTTCGATTTTGAATTAGCATTTCCTACAAATTTCACTTTTTATTGAAGTTTCCTTTTTAGTAGTGGACAACGTGGGTGTTCTGGATTTTTTTTTTTTAATATTTTTCTGCTGTGACCCAACAAATATGTTGTACTGCTTTATGTATTTTTGCAGAGAAGGACTGGGATGACATAAAATTTGGAGTTGACAACAAAGTTGATTTTTATGCCGTCTCTTTCGTTAAAGATGCACAAGTGGTTCATGAGTTGAAGAATTATCTTAAAAGTAAACAACTGCATATCTATGTTGTGTTTATAAAGTCGATTTGGATGCTAATAAATTGTCTGACTCTGCAGGCTGTGATGCTGACATTCATATAATTGTAAAAATAGAAAGCGCAGACTCCATACCAAATTTGCATTCAATTATTACAGCATCTGATGGGGTAATTATTAGATTTCTTTACAGAATCGGACTGGATCTTCATGAGTTAATGTTGAATTCAGTTCAAGCTTTTTCAATTTGGTTGATACTTTGGGGCTTGATTGCTAATTTAAGGGGCAATCCCTTTTATCATTCTAGGCAATGGTTGCAAGAGGTGATCTTGGTGCGGAACTCCCTATTGAAGAGGTTCCACTTCTGCAGGTAGTTAATGTTTCCCAATGAAAGAGAGGCAGCGGAGATTATTTCCCCTTGTGCCTTTTTTTCACTCTTAGAAGAGATGTTTAGTGTTTTATTCGCCAAACTTCCTAAACATGCTTGATTGAAATGAAGGAGGAGATAATAAACATGTGTCGTGGCATGGGGAAGGCTGTCATTGTGGCAACTAACATGCTTGAGAGTATGATTGTTCATCCAACACCAACCAGAGCAGAAGTATCTGACATTGCTATTGCTGTTAGAGAGGGTGCTGATGCAGTCATGCTTTCTGGAGAAACTGCTCACGGAAAGTGAGTTATTATTTTGAGTTCCTTAGTACATTAGTTAGAACATGTGATTGGCCCATATTTTTGGGGCATATTATATTGTTTTGTGCACTTAGGAATTTTTGGGTCAGATTATGTTGTTTCTTTAGATCTTGCGATGGTTCCCAAGATTAAGTGTTCTACGGTGGGTACAGAATGATATGATTCTGCAAAACAATAATTCATATTGATTAATTGGTTTTTAAAAGCTATAAGGCATGATACGTGCAGATATTGAGCGCATTACGAAATAAAATAGGAATAAATAAGTATGTTCTTGCATTTTGAACACCGACTTTGCCCATTTACAGATTCCCATTAAAAGCTGTGAAAGTAATGCATACAGTTGCTTTACGGACTGAAGCGACCATAGCCGGTGGTCAAATGCCATTGAATCTCGGCCAAACATTCAAGGTTGTTTAAAAATTAGTAATTATTTAATACTTCAGTAGGAACATAATTTTGTGCTCTTTCCACTCTTCTGTTTTTTCATTAGTTCTATTTCTCCATGACATTGACTTGGTTACTTTTCTTTATGTAGAACCATATGAGCGAGATGTTTGCTTATCATGCAACAATGATGTCCAACACTCTTGGAACCTCAATAGTTGTCTTCACTAGAACTGGTTTTATGGCTATCCTCCTTAGCCATTATCGACCATCTGGCACAACATTTGCCTTTACAAACGAGTGAGTTGCTTGTGTACTTGAAAGCCAATCATATATCATGTCACCTAGATACGATTGCATTCAAGATTCTGGTTCTTTTCCTTTTTGTTGTTTTTTATTTTATTGTCTGACAGAACCAAAACTTGTTTTCATCGAACTGCTTTATTATTCTCTAGTCTCCTATTTCTTTCCTCCACATTTATGTGTCAATACTTCATGATCTTGATAGTTCTTTCTATTGTGCTCAAAGTATTGGCATAAGGTGCTCTTATTGATGTAGGCATCACATACAAAGATACAAAAATTCGAGTCCATTATTGATATAAATGTTTGCCATTTCAATATTGGTCCACTGAGTTAATCTGCATCTAGTAGGATCTTATAGAAAAGGCTAGTATCATGGATGAAAATGCTTTCTTCCTGTATGTGGCCAAAGGAGTAATTCATGAGTAGGAAATATAAAATTTAGTCGGGTTCTTGGATTTTAGTTATGATAGTATCACTATCGTTAAATACCCTACTGTGCCATGCAGTAAAACAATACAACAGAGGTTGGCTTTATATCAGGGAGTGTGCCCCATATACATGCAGTTCTCTGATGATGCTGAGAAGACCTTTGCTGATGCCTTATCTCTGCTTCAGGTATGGGTCCTATCTGCAATTTGTTTTCTCTTTTCGCTTCTTTTATCTGGAGGGAACCTTTTTAATGCCTTCTCTGAAATAGTACGAATACTATGTTTCGTTTCCATTTAGCAATGATTTTATTGCATAATATGGCAGGTTATTATAACTTAGAAGGTGCAGGGTTGTAGTTTAAATTATTTATTATTCTTGTCCTCGTGTATTCTCCAGTAATATGGTGATGCATATGGTCATTGTTCACTTAGATGACCATTTAAGAAAGTTTGATCTTCAACTTGGAAATCTTCATGGAAGCTTTTTCAAATTCTATCCCAACAGTTTTACCACATCTCTAACTAATCCCAAAAATACCAATCAGTGGGAAGAACCCCAAATAAGAAAGGTACTAGTAACTTGAGAGACATTGTCCTTGAAATCAAAACTGAGTGGATCTCGACTGAACTTTCCCCTGATATTGCCATCGGCAGATGAAAGAGATGCGATCTCCGTACTATAATTCCACTTGATCTGCACTTAAATTTTCTGGTGAGCATGTACCTTGTTCTGACTCATTAAGTTTGTTGTGTACAGAGGCAAGGAATGGTGAAGGAAGGAGAAGAGGTAGCACTCCTTCAGAGTGGCAGACAACCCATATGGCGGTTCCCATCTACTCACAATATTCAAGTGCGTAAAGTGTAG

mRNA sequence

ATGTCTCAGGTGGTGGCTACAAGGTCGATTTGCAGCACATTCCTGTGCCCTAGCTCGGGATCTGTGCAGGACCGGGCGGACAAGGCTCGGTCTTTTGGTTTTGGCTCGAAAGTGCTGGCGCACGAGAAGAAGAGTTGGAACTTTGTTTCCAGGAGAAGTCTAGTCACAGCGAAAAGAGCTGCTCAAGCTGCAGTTGTTCCAGTATCACCTGAGGATGTTCAGAAGGCAGATGAGCAATTTGAACATTTACGAGCAGTTCAACAACTTGGTGATACACCGGTTGGAATGTGGTCCAAACCAACAGTTAGACGTAAGACAAAGATTGTGTGCACGATTGGTCCTTCGACTAACACGAAGGAAATGATTTGGAAACTGGCCGAGGCTGGAATGAATGTTGCTCGATTGAATATGTCTCATGGAGACCATGCATCTCATCAGAAAGTTATAGATTTAGTCAGAGAATACAATGCGCAATCACAAGACAACTGCATTGCGATCATGCTCGACACCAAGGGTCCTGAGGTTAGGAGTGGTGACCTACCACAACCAATATTGTTAGAGACTGGACAGGAATTCACATTTTCCATACGGAGAGGTGTTAGTACTCCAGATTCTGTCAGCGTAAACTATGACGATTTTGTTAATGATGTAGAAGTTGGGGACATGCTTCTTGTTGATGGTGGAATGATGTCATTGATGGTGAAGTCTAAGACAGAGGATTCGGTGAAGTGTGAAGTTGTTGATGGAGGAGAGCTCAAGTCCAGACGACATTTAAATGTTCGAGGGAAAAGTGCAACACTGCCTTCCATTACTGAGAAGGACTGGGATGACATAAAATTTGGAGTTGACAACAAAGTTGATTTTTATGCCGTCTCTTTCGTTAAAGATGCACAAGTGGTTCATGAGTTGAAGAATTATCTTAAAAGCTGTGATGCTGACATTCATATAATTGTAAAAATAGAAAGCGCAGACTCCATACCAAATTTGCATTCAATTATTACAGCATCTGATGGGGCAATGGTTGCAAGAGGTGATCTTGGTGCGGAACTCCCTATTGAAGAGGTTCCACTTCTGCAGGAGGAGATAATAAACATGTGTCGTGGCATGGGGAAGGCTGTCATTGTGGCAACTAACATGCTTGAGAGTATGATTGTTCATCCAACACCAACCAGAGCAGAAGTATCTGACATTGCTATTGCTGTTAGAGAGGGTGCTGATGCAGTCATGCTTTCTGGAGAAACTGCTCACGGAAAATTCCCATTAAAAGCTGTGAAAGTAATGCATACAGTTGCTTTACGGACTGAAGCGACCATAGCCGGTGGTCAAATGCCATTGAATCTCGGCCAAACATTCAAGAACCATATGAGCGAGATGTTTGCTTATCATGCAACAATGATGTCCAACACTCTTGGAACCTCAATAGTTGTCTTCACTAGAACTGGTTTTATGGCTATCCTCCTTAGCCATTATCGACCATCTGGCACAACATTTGCCTTTACAAACGATAAAACAATACAACAGAGGTTGGCTTTATATCAGGGAGTGTGCCCCATATACATGCAGTTCTCTGATGATGCTGAGAAGACCTTTGCTGATGCCTTATCTCTGCTTCAGAGGCAAGGAATGGTGAAGGAAGGAGAAGAGGTAGCACTCCTTCAGAGTGGCAGACAACCCATATGGCGGTTCCCATCTACTCACAATATTCAAGTGCGTAAAGTGTAG

Coding sequence (CDS)

ATGTCTCAGGTGGTGGCTACAAGGTCGATTTGCAGCACATTCCTGTGCCCTAGCTCGGGATCTGTGCAGGACCGGGCGGACAAGGCTCGGTCTTTTGGTTTTGGCTCGAAAGTGCTGGCGCACGAGAAGAAGAGTTGGAACTTTGTTTCCAGGAGAAGTCTAGTCACAGCGAAAAGAGCTGCTCAAGCTGCAGTTGTTCCAGTATCACCTGAGGATGTTCAGAAGGCAGATGAGCAATTTGAACATTTACGAGCAGTTCAACAACTTGGTGATACACCGGTTGGAATGTGGTCCAAACCAACAGTTAGACGTAAGACAAAGATTGTGTGCACGATTGGTCCTTCGACTAACACGAAGGAAATGATTTGGAAACTGGCCGAGGCTGGAATGAATGTTGCTCGATTGAATATGTCTCATGGAGACCATGCATCTCATCAGAAAGTTATAGATTTAGTCAGAGAATACAATGCGCAATCACAAGACAACTGCATTGCGATCATGCTCGACACCAAGGGTCCTGAGGTTAGGAGTGGTGACCTACCACAACCAATATTGTTAGAGACTGGACAGGAATTCACATTTTCCATACGGAGAGGTGTTAGTACTCCAGATTCTGTCAGCGTAAACTATGACGATTTTGTTAATGATGTAGAAGTTGGGGACATGCTTCTTGTTGATGGTGGAATGATGTCATTGATGGTGAAGTCTAAGACAGAGGATTCGGTGAAGTGTGAAGTTGTTGATGGAGGAGAGCTCAAGTCCAGACGACATTTAAATGTTCGAGGGAAAAGTGCAACACTGCCTTCCATTACTGAGAAGGACTGGGATGACATAAAATTTGGAGTTGACAACAAAGTTGATTTTTATGCCGTCTCTTTCGTTAAAGATGCACAAGTGGTTCATGAGTTGAAGAATTATCTTAAAAGCTGTGATGCTGACATTCATATAATTGTAAAAATAGAAAGCGCAGACTCCATACCAAATTTGCATTCAATTATTACAGCATCTGATGGGGCAATGGTTGCAAGAGGTGATCTTGGTGCGGAACTCCCTATTGAAGAGGTTCCACTTCTGCAGGAGGAGATAATAAACATGTGTCGTGGCATGGGGAAGGCTGTCATTGTGGCAACTAACATGCTTGAGAGTATGATTGTTCATCCAACACCAACCAGAGCAGAAGTATCTGACATTGCTATTGCTGTTAGAGAGGGTGCTGATGCAGTCATGCTTTCTGGAGAAACTGCTCACGGAAAATTCCCATTAAAAGCTGTGAAAGTAATGCATACAGTTGCTTTACGGACTGAAGCGACCATAGCCGGTGGTCAAATGCCATTGAATCTCGGCCAAACATTCAAGAACCATATGAGCGAGATGTTTGCTTATCATGCAACAATGATGTCCAACACTCTTGGAACCTCAATAGTTGTCTTCACTAGAACTGGTTTTATGGCTATCCTCCTTAGCCATTATCGACCATCTGGCACAACATTTGCCTTTACAAACGATAAAACAATACAACAGAGGTTGGCTTTATATCAGGGAGTGTGCCCCATATACATGCAGTTCTCTGATGATGCTGAGAAGACCTTTGCTGATGCCTTATCTCTGCTTCAGAGGCAAGGAATGGTGAAGGAAGGAGAAGAGGTAGCACTCCTTCAGAGTGGCAGACAACCCATATGGCGGTTCCCATCTACTCACAATATTCAAGTGCGTAAAGTGTAG

Protein sequence

MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRAAQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVVHELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Homology
BLAST of Moc10g01010.1 vs. NCBI nr
Match: XP_022140989.1 (plastidial pyruvate kinase 2 [Momordica charantia])

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA
Sbjct: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420
           EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP
Sbjct: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Moc10g01010.1 vs. NCBI nr
Match: XP_038874991.1 (plastidial pyruvate kinase 2 [Benincasa hispida])

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 546/573 (95.29%), Postives = 560/573 (97.73%), Query Frame = 0

Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQ+R D+AR FG GSKV AHEKKSWNFV RRSL+T+KRA
Sbjct: 1   MSQVVATRSINSTFLCPSSGSVQERVDRARLFGIGSKVFAHEKKSWNFVYRRSLITSKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQA VVPVSPEDVQKADEQFEH+RAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHIRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLE+GQEFTF+IRRGVST DSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELK YLKSCDADIH+IVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420
           EIIN+CR MGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGETAHGKFP
Sbjct: 361 EIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQFSDDAE+TF DALS+LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFTDALSMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Moc10g01010.1 vs. NCBI nr
Match: XP_008439317.1 (PREDICTED: plastidial pyruvate kinase 2 [Cucumis melo])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 546/573 (95.29%), Postives = 561/573 (97.91%), Query Frame = 0

Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQ+R D+ARSFG GSKVLAHEKKSWN   RRSL+T+KRA
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQERLDRARSFGTGSKVLAHEKKSWNLSYRRSLITSKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLETGQEFTF+IRRGVST D+VSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLETGQEFTFTIRRGVSTADTVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELK YLKSCDADIH+IVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420
           EIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQFS+DAE+TF DAL++LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSEDAEQTFTDALTMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Moc10g01010.1 vs. NCBI nr
Match: TYK23021.1 (plastidial pyruvate kinase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 545/573 (95.11%), Postives = 560/573 (97.73%), Query Frame = 0

Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQ+R D+ARSFG GSKVLAHEKKSWN   RRSL+T+KRA
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQERLDRARSFGTGSKVLAHEKKSWNLSYRRSLITSKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLETGQEFTF+IRRGVST D+VSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLETGQEFTFTIRRGVSTADTVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELK YLKSCDADIH+IVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420
           EIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
            KAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 FKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQFS+DAE+TF DAL++LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSEDAEQTFTDALTMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Moc10g01010.1 vs. NCBI nr
Match: XP_023552329.1 (plastidial pyruvate kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 542/573 (94.59%), Postives = 560/573 (97.73%), Query Frame = 0

Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQDR D+ARSFG GSK+L HEKKSWNF  RRSL+T+K A
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQDRVDRARSFGIGSKLLGHEKKSWNFAHRRSLITSKGA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
            QA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPST+TKE
Sbjct: 61  TQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTDTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLE+GQEFTF+IRRGVST DSVSVNYDDFVNDVEVGDM+LVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDMILVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELK YLKSCDADIH+IVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420
           EIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCP+YMQFSDDAE+TF+DALS+LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPLYMQFSDDAEQTFSDALSMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVAL+QSG+QPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALVQSGKQPIWRFPSTHNIQVRKV 573

BLAST of Moc10g01010.1 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 3.3e-266
Identity = 470/579 (81.17%), Postives = 514/579 (88.77%), Query Frame = 0

Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADK-ARSFGFGSKVLAHEKKSWNFV---SRRSLVT 60
           M+QVVATRSI  + L P+ GSV  R++K  +   F  KVL +E K    V   SRR + T
Sbjct: 1   MAQVVATRSIQGSMLSPNGGSVSTRSEKLLKPASFAVKVLGNEAKRSGRVSVRSRRVVDT 60

Query: 61  AKRAA--QAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGP 120
             R+A  +  V+PVSPEDV   +EQ E L  +QQ GDT VGMWSKPTVRRKTKIVCT+GP
Sbjct: 61  TVRSARVETEVIPVSPEDVPNREEQLERLLEMQQFGDTSVGMWSKPTVRRKTKIVCTVGP 120

Query: 121 STNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPE 180
           STNT+EMIWKLAEAGMNVAR+NMSHGDHASH+KVIDLV+EYNAQ++DN IAIMLDTKGPE
Sbjct: 121 STNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPE 180

Query: 181 VRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMV 240
           VRSGDLPQPI+L+ GQEFTF+I RGVSTP  VSVNYDDFVNDVE GDMLLVDGGMMS MV
Sbjct: 181 VRSGDLPQPIMLDPGQEFTFTIERGVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMV 240

Query: 241 KSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFV 300
           KSKT+DSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDW+DIKFGV+NKVDFYAVSFV
Sbjct: 241 KSKTKDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFV 300

Query: 301 KDAQVVHELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE 360
           KDAQVVHELK YL++  ADIH+IVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE
Sbjct: 301 KDAQVVHELKKYLQNSGADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE 360

Query: 361 VPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGET 420
           VP+LQEEIIN+CR MGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGET
Sbjct: 361 VPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGET 420

Query: 421 AHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSI 480
           AHGKFPLKA  VMHTVALRTEATI  G+MP NLGQ FKNHMSEMFAYHATMMSNTLGTS 
Sbjct: 421 AHGKFPLKAAGVMHTVALRTEATITSGEMPPNLGQAFKNHMSEMFAYHATMMSNTLGTST 480

Query: 481 VVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADAL 540
           VVFTRTGFMAILLSHYRPSGT +AFTN+K IQQRLALYQGVCPIYM+F+DDAE+TFA+AL
Sbjct: 481 VVFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANAL 540

Query: 541 SLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           + L +QGMVK+GEE+A++QSG QPIWR  STHNIQVRKV
Sbjct: 541 ATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

BLAST of Moc10g01010.1 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 1.4e-211
Identity = 367/470 (78.09%), Postives = 418/470 (88.94%), Query Frame = 0

Query: 104 RKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNC 163
           RKTKIVCTIGPST+++EMIWKLAEAGMNVARLNMSHGDHASHQ+ IDLV+EYNAQ +D  
Sbjct: 91  RKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDKV 150

Query: 164 IAIMLDTKGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDML 223
           IAIMLDTKGPEV SGD+P+PILL+ GQEF FSI+RGVST D+VSVNYDDF+NDVE GD+L
Sbjct: 151 IAIMLDTKGPEVISGDVPKPILLKEGQEFNFSIKRGVSTEDTVSVNYDDFINDVEAGDIL 210

Query: 224 LVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVD 283
           LVDGGMMSL VKSKT D VKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGV+
Sbjct: 211 LVDGGMMSLAVKSKTSDIVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVN 270

Query: 284 NKVDFYAVSFVKDAQVVHELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVAR 343
           N+VDFYAVSFVKDA+VVHELK+YLKSC+ADIH+IVKIESADSIPNLHSII+ASDGAMVAR
Sbjct: 271 NQVDFYAVSFVKDAKVVHELKDYLKSCNADIHVIVKIESADSIPNLHSIISASDGAMVAR 330

Query: 344 GDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVRE 403
           GDLGAELPIEEVPLLQE+II  C+ M K VIVATNMLESMI HPTPTRAEVSDI+IAVRE
Sbjct: 331 GDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPTPTRAEVSDISIAVRE 390

Query: 404 GADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHA 463
           GADAVMLSGETAHGK+PLKAVKVMH VALRTE+++       +    +K+HM EMFA+H+
Sbjct: 391 GADAVMLSGETAHGKYPLKAVKVMHIVALRTESSLQKSTSSPSQSAAYKSHMGEMFAFHS 450

Query: 464 TMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFS 523
           + M+NTL T I+VFTRTG MAI+LSH RPS T FAFTN++ ++QRLALY GV PIYM+FS
Sbjct: 451 SSMANTLSTPIIVFTRTGSMAIILSHNRPSSTVFAFTNNERVKQRLALYHGVVPIYMEFS 510

Query: 524 DDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
            DAE+TF+ A+ LL  + +VK+G+ V L+QSG QPIWR  STH+IQVRKV
Sbjct: 511 SDAEETFSRAIKLLLSKSLVKDGQYVTLVQSGAQPIWRRHSTHHIQVRKV 560

BLAST of Moc10g01010.1 vs. ExPASy Swiss-Prot
Match: Q93Z53 (Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP3 PE=1 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 6.9e-208
Identity = 366/471 (77.71%), Postives = 411/471 (87.26%), Query Frame = 0

Query: 103 RRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDN 162
           RRKTKIVCTIGPS++++EMIWKLAEAGMNVARLNMSHGDHASHQ  IDLV+EYN+   D 
Sbjct: 98  RRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDK 157

Query: 163 CIAIMLDTKGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDM 222
            IAIMLDTKGPEVRSGD+PQPI LE GQEF F+I+RGVS  D+VSVNYDDFVNDVEVGD+
Sbjct: 158 AIAIMLDTKGPEVRSGDVPQPIFLEEGQEFNFTIKRGVSLKDTVSVNYDDFVNDVEVGDI 217

Query: 223 LLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGV 282
           LLVDGGMMSL VKSKT D VKC V+DGGEL+SRRHLNVRGKSATLPSIT+KDW+DIKFGV
Sbjct: 218 LLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLNVRGKSATLPSITDKDWEDIKFGV 277

Query: 283 DNKVDFYAVSFVKDAQVVHELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVA 342
           DN+VDFYAVSFVKDA+VVHELKNYLK+C ADI +IVKIESADSI NL SII+A DGAMVA
Sbjct: 278 DNQVDFYAVSFVKDAKVVHELKNYLKTCSADISVIVKIESADSIKNLPSIISACDGAMVA 337

Query: 343 RGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVR 402
           RGDLGAELPIEEVPLLQEEII  CR + K VIVATNMLESMI HPTPTRAEVSDIAIAVR
Sbjct: 338 RGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTPTRAEVSDIAIAVR 397

Query: 403 EGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYH 462
           EGADA+MLSGETAHGKFPLKAV VMHTVALRTEA++   +   +    +K HM +MFA+H
Sbjct: 398 EGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLP-VRTSASRTTAYKGHMGQMFAFH 457

Query: 463 ATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQF 522
           A++M+NTL + ++VFTRTG MA+LLSHYRPS T FAFTN + I QRLALYQGV PIYM+F
Sbjct: 458 ASIMANTLSSPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQRLALYQGVMPIYMEF 517

Query: 523 SDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           SDDAE T+A +L LLQ + M+KEG+ V L+QSG QPIWR  STH IQVRK+
Sbjct: 518 SDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREESTHLIQVRKI 567

BLAST of Moc10g01010.1 vs. ExPASy Swiss-Prot
Match: P55964 (Pyruvate kinase isozyme G, chloroplastic (Fragment) OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 7.2e-181
Identity = 325/418 (77.75%), Postives = 364/418 (87.08%), Query Frame = 0

Query: 156 NAQSQDNCIAIMLDTKGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVN 215
           NAQS DN ++IMLDTKGPEVRSGD+PQP+L E GQEF  +IRRGVST D+VSVNYDDFVN
Sbjct: 1   NAQSHDNVVSIMLDTKGPEVRSGDVPQPMLKE-GQEFNPTIRRGVSTQDTVSVNYDDFVN 60

Query: 216 DVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDW 275
           DV VGD+LLVDGGMMSL VKSKT D VKC VVDGGELKSRRHLNVRGKSA LPSIT+KDW
Sbjct: 61  DVVVGDILLVDGGMMSLAVKSKTSDLVKCVVVDGGELKSRRHLNVRGKSARLPSITDKDW 120

Query: 276 DDIKFGVDNKVDFYAVSFVKDAQVVHELKNYLKSCDADIHIIVKIESADSIPNLHSIITA 335
            DIKFGVDN+VDFYAVSFVKDA+VVHELK YLK C+ADIH+IVKIESADSIPNLHSII+A
Sbjct: 121 GDIKFGVDNQVDFYAVSFVKDAKVVHELKEYLKRCNADIHVIVKIESADSIPNLHSIISA 180

Query: 336 SDGAMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVS 395
           SDGAMVARGDLGAELPIEEVPLLQE+II  C  M K VIVATNMLESMI HPTPTRAEVS
Sbjct: 181 SDGAMVARGDLGAELPIEEVPLLQEDIIRRCHSMQKPVIVATNMLESMINHPTPTRAEVS 240

Query: 396 DIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHM 455
           DIAIAVREGADAVMLSGETAHGK+PLKAV+VMHTVALRTE++      P   G  +K HM
Sbjct: 241 DIAIAVREGADAVMLSGETAHGKYPLKAVRVMHTVALRTESSSPVNTTPPAQG-AYKGHM 300

Query: 456 SEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGV 515
            EMFA+HAT+M+NTL T I+VFTRTG MA+LLSHY+P+ T FAFTN++ I+QRL+LY+GV
Sbjct: 301 GEMFAFHATIMANTLNTPIIVFTRTGSMAVLLSHYQPASTIFAFTNEERIKQRLSLYRGV 360

Query: 516 CPIYMQFSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
            PIYM+FS DAE+TF+ AL LL  +G++ EGE V L+QSG QPIWR  STH+IQVRKV
Sbjct: 361 MPIYMEFSSDAEETFSRALQLLLNKGLLVEGEHVTLVQSGAQPIWRQESTHHIQVRKV 416

BLAST of Moc10g01010.1 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 6.1e-103
Identity = 219/476 (46.01%), Postives = 304/476 (63.87%), Query Frame = 0

Query: 104 RKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNC 163
           R+TK+VCTIGP+T   E +  LA  GMNVAR+NM HG    H+ VI+ VR  N + +   
Sbjct: 104 RRTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTREWHKSVIERVRRLN-EEKGFA 163

Query: 164 IAIMLDTKGPEVRSGDL--PQPILLETGQEFTFSIRRGVS-TPD-SVSVNYDDFVNDVEV 223
           +AIM+DT+G E+  GDL        E G+ +TFS+R   S  P+ +++VNYD F  DV+V
Sbjct: 164 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDSPRPERTINVNYDGFAEDVKV 223

Query: 224 GDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSITE 283
           GD LLVDGGM+   V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 224 GDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISS 283

Query: 284 KDWDDIKFGVDNKVDFYAVSFVKDAQVVHELKNYL--KSCDADIHIIVKIESADSIPNLH 343
           KDW DI FG+   VDF A+SFVK A+V++ LK+Y+  +S D+DI +I KIES DS+ NL 
Sbjct: 284 KDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSRDSDIAVIAKIESIDSLKNLE 343

Query: 344 SIITASDGAMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPT 403
            II ASDGAMVARGDLGA++P+E+VP  Q+ I+ +CR + K VIVA+ +LESMI +PTPT
Sbjct: 344 EIIRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLNKPVIVASQLLESMIEYPTPT 403

Query: 404 RAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVAL------RTEATIAGGQMP 463
           RAEV+D++ AVR+ ADA+MLSGE+A G++P KA+ V+ +V++      R E      ++P
Sbjct: 404 RAEVADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSVSVRIEKWWREEKHHEAMELP 463

Query: 464 LNLGQTFKNHMSEMFAYHATMMSNTLGT-SIVVFTRTGFMAILLSHYRPSGTTFAFTNDK 523
             +G T+ + +SE     A  M+N LG  ++ V+T+ G MA LLS  RP    FAFT   
Sbjct: 464 A-IGSTYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMASLLSRCRPDCPIFAFTTTT 523

Query: 524 TIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPI 560
           ++++RL L  G+ P  + F+DD E       SLL+ +GM+K G+ V  +    Q I
Sbjct: 524 SVRRRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIKSGDLVIAVSDMLQSI 577

BLAST of Moc10g01010.1 vs. ExPASy TrEMBL
Match: A0A6J1CHB6 (Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111011509 PE=3 SV=1)

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA
Sbjct: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420
           EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP
Sbjct: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Moc10g01010.1 vs. ExPASy TrEMBL
Match: A0A1S3AY45 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103484139 PE=3 SV=1)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 546/573 (95.29%), Postives = 561/573 (97.91%), Query Frame = 0

Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQ+R D+ARSFG GSKVLAHEKKSWN   RRSL+T+KRA
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQERLDRARSFGTGSKVLAHEKKSWNLSYRRSLITSKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLETGQEFTF+IRRGVST D+VSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLETGQEFTFTIRRGVSTADTVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELK YLKSCDADIH+IVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420
           EIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQFS+DAE+TF DAL++LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSEDAEQTFTDALTMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Moc10g01010.1 vs. ExPASy TrEMBL
Match: A0A5D3DH87 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G001310 PE=3 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 545/573 (95.11%), Postives = 560/573 (97.73%), Query Frame = 0

Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQ+R D+ARSFG GSKVLAHEKKSWN   RRSL+T+KRA
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQERLDRARSFGTGSKVLAHEKKSWNLSYRRSLITSKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLETGQEFTF+IRRGVST D+VSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLETGQEFTFTIRRGVSTADTVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELK YLKSCDADIH+IVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420
           EIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
            KAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 FKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQFS+DAE+TF DAL++LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSEDAEQTFTDALTMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Moc10g01010.1 vs. ExPASy TrEMBL
Match: A0A6J1E5B9 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430719 PE=3 SV=1)

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 542/573 (94.59%), Postives = 560/573 (97.73%), Query Frame = 0

Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQDR D+ARSFG GSK+L HEKKSWNF  RRSL+T+K A
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQDRVDRARSFGIGSKLLGHEKKSWNFAYRRSLITSKGA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
            QA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPST+TKE
Sbjct: 61  TQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTDTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLE+GQEFTF+IRRGVST DSVSVNYDDFVNDVEVGDM+LVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDMILVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELK YLKSCDADIH+IVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420
           EIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCP+YMQFSDDAE+TF+DALS+LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPLYMQFSDDAEQTFSDALSMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVAL+QSG+QPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALVQSGKQPIWRFPSTHNIQVRKV 573

BLAST of Moc10g01010.1 vs. ExPASy TrEMBL
Match: A0A6J1J4F4 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482565 PE=3 SV=1)

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 542/573 (94.59%), Postives = 559/573 (97.56%), Query Frame = 0

Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSV DR D+ARSFG GSK+L HEKKSWNF  RRSL+T+K A
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVHDRVDRARSFGIGSKLLRHEKKSWNFAYRRSLITSKGA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
            QA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPST+TKE
Sbjct: 61  TQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTDTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLE+GQEFTF+IRRGVST DSVSVNYDDFVNDVEVGDM+LVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDMILVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELK YLKSCDADIH+IVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420
           EIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQFSDDAE+TF+DALS+LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFSDALSMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVAL+QSG+QPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALVQSGKQPIWRFPSTHNIQVRKV 573

BLAST of Moc10g01010.1 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 918.7 bits (2373), Expect = 2.3e-267
Identity = 470/579 (81.17%), Postives = 514/579 (88.77%), Query Frame = 0

Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADK-ARSFGFGSKVLAHEKKSWNFV---SRRSLVT 60
           M+QVVATRSI  + L P+ GSV  R++K  +   F  KVL +E K    V   SRR + T
Sbjct: 1   MAQVVATRSIQGSMLSPNGGSVSTRSEKLLKPASFAVKVLGNEAKRSGRVSVRSRRVVDT 60

Query: 61  AKRAA--QAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGP 120
             R+A  +  V+PVSPEDV   +EQ E L  +QQ GDT VGMWSKPTVRRKTKIVCT+GP
Sbjct: 61  TVRSARVETEVIPVSPEDVPNREEQLERLLEMQQFGDTSVGMWSKPTVRRKTKIVCTVGP 120

Query: 121 STNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPE 180
           STNT+EMIWKLAEAGMNVAR+NMSHGDHASH+KVIDLV+EYNAQ++DN IAIMLDTKGPE
Sbjct: 121 STNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPE 180

Query: 181 VRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMV 240
           VRSGDLPQPI+L+ GQEFTF+I RGVSTP  VSVNYDDFVNDVE GDMLLVDGGMMS MV
Sbjct: 181 VRSGDLPQPIMLDPGQEFTFTIERGVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMV 240

Query: 241 KSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFV 300
           KSKT+DSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDW+DIKFGV+NKVDFYAVSFV
Sbjct: 241 KSKTKDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFV 300

Query: 301 KDAQVVHELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE 360
           KDAQVVHELK YL++  ADIH+IVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE
Sbjct: 301 KDAQVVHELKKYLQNSGADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE 360

Query: 361 VPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGET 420
           VP+LQEEIIN+CR MGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGET
Sbjct: 361 VPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGET 420

Query: 421 AHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSI 480
           AHGKFPLKA  VMHTVALRTEATI  G+MP NLGQ FKNHMSEMFAYHATMMSNTLGTS 
Sbjct: 421 AHGKFPLKAAGVMHTVALRTEATITSGEMPPNLGQAFKNHMSEMFAYHATMMSNTLGTST 480

Query: 481 VVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADAL 540
           VVFTRTGFMAILLSHYRPSGT +AFTN+K IQQRLALYQGVCPIYM+F+DDAE+TFA+AL
Sbjct: 481 VVFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANAL 540

Query: 541 SLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           + L +QGMVK+GEE+A++QSG QPIWR  STHNIQVRKV
Sbjct: 541 ATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

BLAST of Moc10g01010.1 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 724.9 bits (1870), Expect = 4.9e-209
Identity = 366/471 (77.71%), Postives = 411/471 (87.26%), Query Frame = 0

Query: 103 RRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDN 162
           RRKTKIVCTIGPS++++EMIWKLAEAGMNVARLNMSHGDHASHQ  IDLV+EYN+   D 
Sbjct: 98  RRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDK 157

Query: 163 CIAIMLDTKGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDM 222
            IAIMLDTKGPEVRSGD+PQPI LE GQEF F+I+RGVS  D+VSVNYDDFVNDVEVGD+
Sbjct: 158 AIAIMLDTKGPEVRSGDVPQPIFLEEGQEFNFTIKRGVSLKDTVSVNYDDFVNDVEVGDI 217

Query: 223 LLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGV 282
           LLVDGGMMSL VKSKT D VKC V+DGGEL+SRRHLNVRGKSATLPSIT+KDW+DIKFGV
Sbjct: 218 LLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLNVRGKSATLPSITDKDWEDIKFGV 277

Query: 283 DNKVDFYAVSFVKDAQVVHELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVA 342
           DN+VDFYAVSFVKDA+VVHELKNYLK+C ADI +IVKIESADSI NL SII+A DGAMVA
Sbjct: 278 DNQVDFYAVSFVKDAKVVHELKNYLKTCSADISVIVKIESADSIKNLPSIISACDGAMVA 337

Query: 343 RGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVR 402
           RGDLGAELPIEEVPLLQEEII  CR + K VIVATNMLESMI HPTPTRAEVSDIAIAVR
Sbjct: 338 RGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTPTRAEVSDIAIAVR 397

Query: 403 EGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYH 462
           EGADA+MLSGETAHGKFPLKAV VMHTVALRTEA++   +   +    +K HM +MFA+H
Sbjct: 398 EGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLP-VRTSASRTTAYKGHMGQMFAFH 457

Query: 463 ATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQF 522
           A++M+NTL + ++VFTRTG MA+LLSHYRPS T FAFTN + I QRLALYQGV PIYM+F
Sbjct: 458 ASIMANTLSSPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQRLALYQGVMPIYMEF 517

Query: 523 SDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           SDDAE T+A +L LLQ + M+KEG+ V L+QSG QPIWR  STH IQVRK+
Sbjct: 518 SDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREESTHLIQVRKI 567

BLAST of Moc10g01010.1 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 373.6 bits (958), Expect = 2.8e-103
Identity = 221/475 (46.53%), Postives = 300/475 (63.16%), Query Frame = 0

Query: 104 RKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNC 163
           R+TK++CTIGP+T   E +  LA  GMNVARLNM HG    H+ VI  VR  N + +   
Sbjct: 117 RRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLN-EEKGFA 176

Query: 164 IAIMLDTKGPEVRSGDL--PQPILLETGQEFTFSIRR-GVSTPD-SVSVNYDDFVNDVEV 223
           +AIM+DT+G E+  GDL        E G+ +TF++R    S P+ ++SV+YD F  DV V
Sbjct: 177 VAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRV 236

Query: 224 GDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSITE 283
           GD LLVDGGM+   V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 237 GDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISS 296

Query: 284 KDWDDIKFGVDNKVDFYAVSFVKDAQVVHELKNYL--KSCDADIHIIVKIESADSIPNLH 343
           KDW DI FG+   VDF AVSFVK A+V++ LK+YL  +S   +I +I KIES DS+ NL 
Sbjct: 297 KDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLE 356

Query: 344 SIITASDGAMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPT 403
            II ASDGAMVARGDLGA++P+E+VP  Q+ I+ +CR + K VIVA+ +LESMI +PTPT
Sbjct: 357 EIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPT 416

Query: 404 RAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVALRTE----ATIAGGQMPLN 463
           RAEV+D++ AVR+ +DA+MLSGE+A G+FP KA+ V+ TV+LR E           +PL 
Sbjct: 417 RAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQ 476

Query: 464 -LGQTFKNHMSEMFAYHATMMSNTLGT-SIVVFTRTGFMAILLSHYRPSGTTFAFTNDKT 523
            +G +F + +SE     A  M+N LG  ++ V+T +G MA L+S  RP    FAFT   +
Sbjct: 477 AIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTTS 536

Query: 524 IQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPI 560
           +++RL L  G+ P  + FSDD E       SLL+ +GM+K G+ V  +    Q I
Sbjct: 537 VRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSI 590

BLAST of Moc10g01010.1 vs. TAIR 10
Match: AT3G25960.1 (Pyruvate kinase family protein )

HSP 1 Score: 273.9 bits (699), Expect = 3.0e-73
Identity = 176/477 (36.90%), Postives = 265/477 (55.56%), Query Frame = 0

Query: 95  GMWSKPTVRRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVRE 154
           G  +   +R KTKIVCT+GP++ + EMI KL +AGMNVAR N SHG H+ HQ+ +D +R 
Sbjct: 7   GQATNGALRSKTKIVCTLGPASRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLRT 66

Query: 155 YNAQSQDNCIAIMLDTKGPEVRSGDLPQ--PILLETGQEFTFSIRRGV-STPDSVSVNYD 214
               +   C A+MLDTK P +R+G L +  PI L+ GQE T SI   +    +++S++Y 
Sbjct: 67  AMDNTGILC-AVMLDTKSPVIRTGFLKEGKPIQLKQGQEITISIDYKIQGDSNTISMSYK 126

Query: 215 DFVNDVEVGDMLLVDGGMMSLMVKS--KTEDSVKCEVVDGGELKSRRHLNVRGKSATLPS 274
               D++ GD++L   G +SL V S  K    V+C   +   L  R+++N+ G    LP+
Sbjct: 127 KLAEDLKPGDVILCSDGTISLNVLSCDKYLGLVRCRCENSALLGERKNVNLPGIVVDLPT 186

Query: 275 ITEKDWDDI-KFGVDNKVDFYAVSFVKDAQVVHELKNYLKSCDADIHIIVKIESADSIPN 334
           +TEKD +DI ++GV NK+D  A+SFV+    + +++  L      I ++ K+E+ + + N
Sbjct: 187 LTEKDKEDIMQWGVPNKIDIIALSFVRKGSDLIQVRKLLGEHSKSIMLMSKVENQEGVMN 246

Query: 335 LHSIITASDGAMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPT 394
              I+  SD  MVARGDLG E+PIE++ L Q+ +IN     GK V+ AT MLESM V P 
Sbjct: 247 FDKILENSDAFMVARGDLGMEIPIEKMFLAQKTMINKANAHGKPVVTATQMLESMTVSPR 306

Query: 395 PTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGG---QMPL 454
           PTRAE +D+A AV +G D VMLSGETA G  P  AV  M  +    E  I      +  L
Sbjct: 307 PTRAEATDVANAVLDGTDCVMLSGETAAGAHPETAVLTMSRICKEAEDFIDYDILHKKTL 366

Query: 455 NLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPS-------GTTFA 514
            +     + +  + A   +   +   ++IVV TR G+ A L++ YRPS           A
Sbjct: 367 GMVSLPLSPIESLAASAVSTARSVFASAIVVLTRGGYTAELVAKYRPSVPILSVIMPEIA 426

Query: 515 FTNDKT--IQQRLALYQGVCPIY-MQFSDDAEKTFADALSLLQRQGMVKEGEEVALL 553
             +D    + +R  +Y+G+ P+      D  E+    A+   + +G+ K G+ +  L
Sbjct: 427 ECSDSVAHVARRGLIYRGIIPVVGCSARDSTEEMIRLAIGFAKTKGICKTGDSIVAL 482

BLAST of Moc10g01010.1 vs. TAIR 10
Match: AT3G55650.1 (Pyruvate kinase family protein )

HSP 1 Score: 272.7 bits (696), Expect = 6.7e-73
Identity = 181/493 (36.71%), Postives = 268/493 (54.36%), Query Frame = 0

Query: 95  GMWSKPTVRRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVRE 154
           G  +   +R KTKI+CT+GP + + EMI KL +AGMNVAR N SHG H+ HQ+ +D +R 
Sbjct: 7   GQATNGALRSKTKIICTLGPVSRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLR- 66

Query: 155 YNAQSQDN---CIAIMLDTKGPEVRSGDLPQ--PILLETGQEFTFSIRRGVSTPDSV-SV 214
               + DN     A+MLDTKGPE+R+G L +  PI L  GQE T SI   +    +V S+
Sbjct: 67  ---TAMDNTGILSAVMLDTKGPEIRTGFLKEGKPIQLNQGQEITISIDYMIEGDSNVISM 126

Query: 215 NYDDFVNDVEVGDMLLVDGGMMSLMVKS--KTEDSVKCEVVDGGELKSRRHLNVRGKSAT 274
           +Y     DV+ GD++L   G +SL V S  K+   V+C   +   L  R+++N+ G    
Sbjct: 127 SYKKLAEDVKPGDVILCSDGTISLTVLSCDKSFGLVRCRCENSAILGERKNVNLPGIVVD 186

Query: 275 LPSITEKDWDD-IKFGVDNKVDFYAVSFVKDAQVVHELKNYLKSCDADIHIIVKIESADS 334
           LP++TEKD +D I++GV NK+D  A+SFV+    + E++  L     +I ++ K+E+ + 
Sbjct: 187 LPTLTEKDKEDIIQWGVPNKIDIIALSFVRKGSDLTEVRRLLGEHSKNIMLMSKVENQEG 246

Query: 335 IPNLHSIITASDGAMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIV 394
           + N   I+  SD  MVARGDLG E+PIE++ L Q+ +I M   +GK V+ AT MLESM V
Sbjct: 247 VMNCEKILENSDAFMVARGDLGMEIPIEKMFLAQKTMIKMANALGKPVVTATQMLESMTV 306

Query: 395 HPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGG---Q 454
            P PTRAE +D+A AV +G D VMLSGETA G  P  AV  M  +    E  I      +
Sbjct: 307 SPRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPEAAVLTMSRICKEAEDFIDYDILHK 366

Query: 455 MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPS-------GT 514
             L +     + +  + A   +   +   ++IVV T+ G+ A L++ YRPS         
Sbjct: 367 KTLGMVSLPLSPIESLAASVVSTAQSVFASAIVVLTKGGYTAELVAKYRPSVPILSVIVP 426

Query: 515 TFAFTNDKTIQ---------QRLALYQGVCPIYMQFS-------DDAEKTFADALSLLQR 553
             A  ND  +          +R  +Y+ + P+    S       D  E+    A+   + 
Sbjct: 427 EIAQGNDMEMSCSDSVAHAARRGLIYRRIIPVVATGSSARDSNKDATEEMINLAIGFAKT 486

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140989.10.0e+00100.00plastidial pyruvate kinase 2 [Momordica charantia][more]
XP_038874991.10.0e+0095.29plastidial pyruvate kinase 2 [Benincasa hispida][more]
XP_008439317.10.0e+0095.29PREDICTED: plastidial pyruvate kinase 2 [Cucumis melo][more]
TYK23021.10.0e+0095.11plastidial pyruvate kinase 2 [Cucumis melo var. makuwa][more]
XP_023552329.10.0e+0094.59plastidial pyruvate kinase 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FLW93.3e-26681.17Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
Q405461.4e-21178.09Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q93Z536.9e-20877.71Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
P559647.2e-18177.75Pyruvate kinase isozyme G, chloroplastic (Fragment) OS=Ricinus communis OX=3988 ... [more]
Q431176.1e-10346.01Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CHB60.0e+00100.00Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111011509 PE=3 SV=1[more]
A0A1S3AY450.0e+0095.29Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103484139 PE=3 SV=1[more]
A0A5D3DH870.0e+0095.11Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G0013... [more]
A0A6J1E5B90.0e+0094.59Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430719 PE=3 SV=1[more]
A0A6J1J4F40.0e+0094.59Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482565 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G52920.12.3e-26781.17plastidic pyruvate kinase beta subunit 1 [more]
AT1G32440.14.9e-20977.71plastidial pyruvate kinase 3 [more]
AT3G22960.12.8e-10346.53Pyruvate kinase family protein [more]
AT3G25960.13.0e-7336.90Pyruvate kinase family protein [more]
AT3G55650.16.7e-7336.71Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 368..392
score: 62.22
coord: 393..411
score: 65.02
coord: 412..428
score: 55.66
coord: 162..178
score: 56.29
coord: 343..367
score: 53.05
coord: 286..300
score: 42.56
coord: 316..342
score: 47.41
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 104..570
e-value: 1.4E-154
score: 513.1
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 35..573
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 445..573
e-value: 1.4E-26
score: 94.5
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 422..573
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 174..267
e-value: 3.6E-132
score: 442.1
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 104..439
e-value: 3.4E-118
score: 394.5
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 106..431
e-value: 3.6E-132
score: 442.1
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 460..553
e-value: 2.7E-14
score: 53.4
NoneNo IPR availablePANTHERPTHR11817:SF2PLASTIDIAL PYRUVATE KINASE 2coord: 35..573
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 314..326
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 95..438
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 173..264

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Moc10g01010Moc10g01010gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Moc10g01010.1.exon12Moc10g01010.1.exon12exon
Moc10g01010.1.exon11Moc10g01010.1.exon11exon
Moc10g01010.1.exon10Moc10g01010.1.exon10exon
Moc10g01010.1.exon9Moc10g01010.1.exon9exon
Moc10g01010.1.exon8Moc10g01010.1.exon8exon
Moc10g01010.1.exon7Moc10g01010.1.exon7exon
Moc10g01010.1.exon6Moc10g01010.1.exon6exon
Moc10g01010.1.exon5Moc10g01010.1.exon5exon
Moc10g01010.1.exon4Moc10g01010.1.exon4exon
Moc10g01010.1.exon3Moc10g01010.1.exon3exon
Moc10g01010.1.exon2Moc10g01010.1.exon2exon
Moc10g01010.1.exon1Moc10g01010.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Moc10g01010.1cds.Moc10g01010.1_12CDS
cds.Moc10g01010.1cds.Moc10g01010.1_11CDS
cds.Moc10g01010.1cds.Moc10g01010.1_10CDS
cds.Moc10g01010.1cds.Moc10g01010.1_9CDS
cds.Moc10g01010.1cds.Moc10g01010.1_8CDS
cds.Moc10g01010.1cds.Moc10g01010.1_7CDS
cds.Moc10g01010.1cds.Moc10g01010.1_6CDS
cds.Moc10g01010.1cds.Moc10g01010.1_5CDS
cds.Moc10g01010.1cds.Moc10g01010.1_4CDS
cds.Moc10g01010.1cds.Moc10g01010.1_3CDS
cds.Moc10g01010.1cds.Moc10g01010.1_2CDS
cds.Moc10g01010.1cds.Moc10g01010.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Moc10g01010.1Moc10g01010.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
molecular_function GO:0003824 catalytic activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity