Moc08g33770.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g33770.1
TypemRNA
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptioneukaryotic initiation factor 4A-3
Locationchr8: 24559820 .. 24565594 (+)
Sequence length1233
RNA-Seq ExpressionMoc08g33770.1
SyntenyMoc08g33770.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCCGCAGCTACGACAAGCGTTGTGCCGCCTAATCGACGGCGATCAATTAATGCGCCGGAAGATAAGTTGGAATTCGAGACGACGGAGGGGGTTGAGCCGATCTCGAGTTTCGATCAGATGGGCATTAAGGACGATCTTCTCCGTGGAATATATGCATACGGGTTCGAGAAGCCCTCGGCCATTCAGCAGAGAGCCGTGAGACCGATTATCGAGGGGCGAGATGTCATTGCTCAGGCCCAGTCTGGAACAGGCAAGACGTCCATGATTGCCCTTACCGTTTGCCAAATGGTTGATACATCGAGTAGAGAGTGAGTATCAGATGTTGAATCTTCCATTTTCTCTCTTTGTCTAGTTCATGTATGTTGTTTAGTTAATTAGCTCAGGAAGCCAATTTCAAAGGCTAAAATTATTTGGATGTTAGTTTATTCCTTTCACAAGAACTTGTTATAACCTCTTGTTGTGGTTTTGGAAATTCTGAATCGAAATTTTCAATTAATAAAGTCTCAACAAAATAACGACACATTCGCTTCTTGTCCTCAAAGTTTGGAACATACATTCAGCTCATGGCGCAGGAGATTTTTCAATTTACATCTGATCTTATTGTTTTATGATCTTTAAAACTTCATTTTCTTATGGTCATCATGCATCTTAATTCCAAACAGAGTACAGGCCTTGATCTTGTCCCCTACGAGAGAATTGGCAACTCAGACAGAGAAAGTAATATTGGCCATTGGCGACTACATTAATATACAAGCACACGCTTGCATTGGCGGCAAAAGTGTAGGTGAAGATATCAGGAAGCTCGAGTTTGGAGTTCAGATTGTATCAGGAACTCCTGGAAGAGTCTGTGACATGATCAAGAGAAGGACATTGCGTACCAGAGCCATTAAGTTGTTAGTTCTGGTAAGTTTGGCTGCCCTTCAGATCGACTCTTTGTTACCATAATAATTAATGTTGTACAGTTTCTTTTGGAAATTTTCATACTTTCTAATTTCATTTTCTAATATTTACTTTTTCAGGATGAGTCTGACGAGATGTTGAGCAGAGGGTTTAAAGACCAGATTTATGATGTGTATAGATATCTCCCACCAGAACTTCAGGTTCTCTCTCTCTCTGTGTGTCTAAGTAATTGTGCCCGTGAGAAGCAGAAGAGGAAAAGTAAATTGAATGCAGTTGTGTCCTTGAATTGGTTGCCAAGCATGACTTTAACCTGTTTAGTATGAAAAATAAGATTTAATATACATGTTTTCATGCACATGCTGACCAATTTGTATGTACAATAACATTCTTTATGCAATAATGCATATAGTTGATTTATTGTTTTGTAAAGGAAACGGTTAAGACATATAAGACTACTGAGAAATAATAGGACTGTTTTACCAAAGTATTTTACAGAATGTCTTTTCATGGTTATTTACAAATCTTTGATGAGGTGGATATGGAGTTCTGTTTATCTTGATTTTAGTTTAGATATTTGCTCATATTTTGTTTCTTTCACGTTTTAGATTTTTTCAATTGCCATTATATGTGCTTGTATGAGTTCTATTAATTTAATACAGTCTATTTAGTTTCATATACTGATTTACCTTTTTGGTTATTGTCAACCAGTTCGTTTTTGCTTGGTTTTATATAGTTTTCTGTTGTTAGGAAGGATCCAGAACAAGATCTCCTGGTTAATTTTATGAGTTTTATCCCAATTTTAATTGCAGGTTGTCTTGATTTCTGCTACCCTTCCTCATGAAATCCTGGAGATGACAAACAAGTTCATGACGGATCCTGTGAGAATCCTTGTCAAGCGTGATGAGTTAACTTTGGAGGTTTGTAATTTTTTTTATTTGTGGAGACTAAATGCCTTTCGGATATATATCAGTACCCTGATGTATGCTGTTAAATTGCCAGGGTATTAAGCAATTCTTCGTTGCAGTTGAAAGGGAAGAGTGGAAGTTTGATACCTTATGTGATCTTTATGACACCTTGACTATCACTCAAGCTGTAATTTTCTGTAACACTAAGCGGAAGGTAATGTGATTTAAAATTTGTTTTTTAGATCTCGTTGAGTCCTCAAAATCTTATTAGGCTCATTCCGAAACAGCTAATTTTATAATTTTGGCCCTCTTATACATGTTTGCTTTTATGCATGATTCTGATATATTCCACTCGTCTAGAATAATATCTTATTATTGCTTAACCTTTGGTCCTTTTCCTTTTTTACTAATCTAATACAAATGTGTCTGGTGACAGAATGATGAGAAAAGTTTATGAGGTTTATATAACTTGACTTTTATTAACTAGAGAGAGAGATACACTGCATAACTGCATAATTATAATTTGAGGGATCCCCTACTCTCATAACTCCCGCTCATTACAACCAAAAATGTATCCTCTCTCTAGAGACCTCATTCTAAATATTTTCTGAAATCATACTCAAGCTAACTATTAGTTACAATGTATCCTACCATCATAACTATTAATCAGGCCTATCCCTTACCCTCTTTGTAGTATGTACTTTTAGTAATTGGAGGCCTATACGAGGTCTAGATCTCATCGCACTGATTCTTGAACTTCAGTTATCAATGCAAAATGTTTCATTTACCCATGCTAAAATATAGTATTAGTACGGGAATAGTTCGCTTCATTTGTTTGAATATTTTATTTATCTTCTGCCTCCAGGTTGAATGGTTAACTGAGAAGATGCGTAGTAATAACTTCACAGTTTCACATATGCATGGGGACATGCCTCAGAGGGAAAGAGATGCAATCATGGGGGAGTTCCGATCAGGAACTACTCGTGTCCTAATCACCACTGATGTTTGGGCACGAGGGCTTGATGTTCAGCAGGCAAGTTGTTGGTTCTTTTACTAATCTATTTTTCTGTCCATGCACCCAAATTCAAGTAACTCTGGTTGTAGTTGTCTCATTTAAATTCAACCTGGTAATCAATGCAGTTATATAAACGGTAATGTGGATCGAGTACTCTATGTTTCTATTTTTATAACTCCTTTAATTGTTGAGTCCTTTCTATAATGTTAGTAAAATTATTATAAGTGGATTTGGAGGTTATATCTTCCCGAATCATTTTTGGATATTCTGTGGATCATATGCACACAGTTCAATCGACTTCTAGTAATGAAGTCATGGACATACGGATAGATTCAGTTTATTCTGGAGTATTTTGTGGATCATATGCCTCAGTTATAGGGTTGTGTAAAAATGAACATCATGGAGAATCATAATGGTTTTATTTGAAGTATTCTCTAATAGAAGGTATATTAGAAAATAACTCTGTATGATGACATAGAGAAGAGAGTGGGTGTTGATTTTGGTAAGCACATATGATAATGTTTTGGCAGTTTACACTATCAATTGGTGTACCCCCCTGTAAATGTTAACAGGCATCATAAATATTTACGTGTTTGTTTTCCATCCACTATCTGATTATGCCAAGTATCTTGAGAAATCTATTAGTACAATTCTTATGTTTATTTGCATTAACTTTTGTCAGGTATCACTAGTGATAAACTACGACCTTCCAAACAATCGAGAACTTTACATTCACAGGATAGGAAGATCTGGTCGGTTTGGGCGCAAGGTATGACATTGTTCTGTATTTCAGTTGATTAATTTTGGAAGAAGTGTTTTTTTAGGTCTAAATATTTTTTAAACACTTTTGGAAGGTAACAAATTAAATGCTTCATCGAAAATCATTTTAAGTGTTCTTTTAATCTATTTTTAGAATTTCTCAACTACTTTTGATTATCGAAAAGTTTAACCAAACAAACTACCACTAATTTAAAAAAAAAACTTTTAGTCCTTTATAAGCACTTTAAAAAGCAGGTCATACATATAGTATTCCACTTGAAAGTAGACATAATGGGTGTAATGTATTGTAAGCAAACTCTAGTTTAGGGAGTTGACTGTGAGAGTCCTATTAGGAGTATAGGGAAATATTAGTTTGTTAGTATGAAGGGGTAGTACGGTAATTAGTATGGTAATTAGTTAGGGTGAGTTGTTATAAATAAGGGTAGTAGGGAGAGGGGAAGGATATCTTGGAGAATTGTATTTTGTGTAAGGAACTTGGGAGAGTTCTTGGGAGAGAGATAGCCCTCTCGAAAGGCTATCGGTTCTTGTATTCTGATACCTAACAAATTGGTATCAGAGCAAGTTCGTCCTGGCCATGAATCTTTCATTCGAGGCAGTTACGAGGATACAGAGATTATTGATAGGAATGGAGTTGCAGGAATTGTTGAGAGAAATGACGGAACGTTCAAATGGAATGGTTCGAATAACGAAAGAAATGCAAAAGGGATTTACTGTTAGAGAGGAGAAAGAGAGATCAAGGAAGGAAGAAGCAACGGCGAAGAGCATTCAGAAGTAGGGACGAAGGATTGTTGGAAGACGGCGATCAGACCCGAGAATGTGGACTGGAGTGCGATGGAAATTCAACCGTGGAGGGAAAGTATTGTTGTGCAGCGATTCATCGAGAAGAGAGGAGAAAAATTACCCTCAACCGAGTATGAGAAGCTAAGGAAGAGAGGAAGAAGGCAACACCGACCACAAAAGTTCGAAAAGGGGGATGAAGAAGAAATTAAAGCCCGAAGAAATACAAAGTCCGGACAAGGTGAACCAATGAATGTTGAGACGAAGGCCGAAATTCGAAGGGAAGTCGACCATGGATGATTTCCATGGAAAAATCGAAAACTCATCGGTGGAAGGAGTTCGAGTTCCAGAAGGGGGACGGAGGAGAAATCGGCGGGCAGAGAAGAAATGTCAAGAGGACGGAGAGCATGAGGTCAAAATCGCAATGTGGCCGACGGGTGAAAAAGCAGTCGATGAAGAACATGGAACGAAGAAGCGGCAGAAGTGGAAATGGTGGCGATGGAGAGGAGTACTAACGAGGGAGGAGACAAGACTCCAGCAAATACAGGGTCGAAGTTTCAAGGCGGTCGGCGAGGGAAGCAGTGTCGGACGAAGTGGCCGAAGGGGTGGAGGCGGTGATGGAAAGGTGAAGTGCGGGGGGGAGGAGAGTTCACGAAGATGAAATTAGGGTTAAACAGATTAAAAAAAGGGCCCAATCAACTGGAAAGGGGCAGCAGACTATTATGGATGATATGGATGATTGGGCCATGGCCTTGGGCAAAAGGAGTGTTGGTGGGTTTTTGGTTTGTTAATATTGGGTTGGGGTTATTTTTGACCTTTTGTTTTCTTTTCGGCGTTTTTGGATGGGTAGTTAAAAAGAAAAAAAAAATTGTTTTTGTGTATTCACACCTTGAGGACAAGGTGTCTTTGAAGGGCGCGGGGTAATGTGAGAGTCTTATTAGGAGTATAGGGGAATATTAGTTTGTTGGTATGAAGGGGTAGTATAGTTATTAGTTAGGGTGAGTTGTTATAAATAAGGGAAGGGTAGTAGGGAGAGGAGAAGGATATATTGGAGAATTGTATTTTGTGTAAGGAACTTGAGAGAGTTCTTGGGAGAGAGATGGCCCTCTCAAAAGGCTATCGGTTCTTGTTAGGTTCTTGTATTCGGATACCTAACACTGACAACGCTGCTGAATGATGTTCTACGTTTCCTTACTGCGGTGTTTGTTAATTTCCTAAACTTGAACTTGGCTGAAACTAAAAACTTTTTTAAGACGAAGTTTGTGTCGTTTAACGCAGGGTGTTGCCATTAACTTTGTGAAAAGCGATGACATAAAGATCCTGAGAGATATTGAGCAGTATTACAGTACCCAGATTGATGAGATGCCCATGAACGTTGCTGATTTGATTTAA

mRNA sequence

ATGGCGGCCGCAGCTACGACAAGCGTTGTGCCGCCTAATCGACGGCGATCAATTAATGCGCCGGAAGATAAGTTGGAATTCGAGACGACGGAGGGGGTTGAGCCGATCTCGAGTTTCGATCAGATGGGCATTAAGGACGATCTTCTCCGTGGAATATATGCATACGGGTTCGAGAAGCCCTCGGCCATTCAGCAGAGAGCCGTGAGACCGATTATCGAGGGGCGAGATGTCATTGCTCAGGCCCAGTCTGGAACAGGCAAGACGTCCATGATTGCCCTTACCGTTTGCCAAATGGTTGATACATCGAGTAGAGAAGTACAGGCCTTGATCTTGTCCCCTACGAGAGAATTGGCAACTCAGACAGAGAAAGTAATATTGGCCATTGGCGACTACATTAATATACAAGCACACGCTTGCATTGGCGGCAAAAGTGTAGGTGAAGATATCAGGAAGCTCGAGTTTGGAGTTCAGATTGTATCAGGAACTCCTGGAAGAGTCTGTGACATGATCAAGAGAAGGACATTGCGTACCAGAGCCATTAAGTTGTTAGTTCTGGATGAGTCTGACGAGATGTTGAGCAGAGGGTTTAAAGACCAGATTTATGATGTGTATAGATATCTCCCACCAGAACTTCAGGTTGTCTTGATTTCTGCTACCCTTCCTCATGAAATCCTGGAGATGACAAACAAGTTCATGACGGATCCTGTGAGAATCCTTGTCAAGCGTGATGAGTTAACTTTGGAGGGTATTAAGCAATTCTTCGTTGCAGTTGAAAGGGAAGAGTGGAAGTTTGATACCTTATGTGATCTTTATGACACCTTGACTATCACTCAAGCTGTAATTTTCTGTAACACTAAGCGGAAGGTTGAATGGTTAACTGAGAAGATGCGTAGTAATAACTTCACAGTTTCACATATGCATGGGGACATGCCTCAGAGGGAAAGAGATGCAATCATGGGGGAGTTCCGATCAGGAACTACTCGTGTCCTAATCACCACTGATGTTTGGGCACGAGGGCTTGATGTTCAGCAGGTATCACTAGTGATAAACTACGACCTTCCAAACAATCGAGAACTTTACATTCACAGGATAGGAAGATCTGGTCGGTTTGGGCGCAAGGGTGTTGCCATTAACTTTGTGAAAAGCGATGACATAAAGATCCTGAGAGATATTGAGCAGTATTACAGTACCCAGATTGATGAGATGCCCATGAACGTTGCTGATTTGATTTAA

Coding sequence (CDS)

ATGGCGGCCGCAGCTACGACAAGCGTTGTGCCGCCTAATCGACGGCGATCAATTAATGCGCCGGAAGATAAGTTGGAATTCGAGACGACGGAGGGGGTTGAGCCGATCTCGAGTTTCGATCAGATGGGCATTAAGGACGATCTTCTCCGTGGAATATATGCATACGGGTTCGAGAAGCCCTCGGCCATTCAGCAGAGAGCCGTGAGACCGATTATCGAGGGGCGAGATGTCATTGCTCAGGCCCAGTCTGGAACAGGCAAGACGTCCATGATTGCCCTTACCGTTTGCCAAATGGTTGATACATCGAGTAGAGAAGTACAGGCCTTGATCTTGTCCCCTACGAGAGAATTGGCAACTCAGACAGAGAAAGTAATATTGGCCATTGGCGACTACATTAATATACAAGCACACGCTTGCATTGGCGGCAAAAGTGTAGGTGAAGATATCAGGAAGCTCGAGTTTGGAGTTCAGATTGTATCAGGAACTCCTGGAAGAGTCTGTGACATGATCAAGAGAAGGACATTGCGTACCAGAGCCATTAAGTTGTTAGTTCTGGATGAGTCTGACGAGATGTTGAGCAGAGGGTTTAAAGACCAGATTTATGATGTGTATAGATATCTCCCACCAGAACTTCAGGTTGTCTTGATTTCTGCTACCCTTCCTCATGAAATCCTGGAGATGACAAACAAGTTCATGACGGATCCTGTGAGAATCCTTGTCAAGCGTGATGAGTTAACTTTGGAGGGTATTAAGCAATTCTTCGTTGCAGTTGAAAGGGAAGAGTGGAAGTTTGATACCTTATGTGATCTTTATGACACCTTGACTATCACTCAAGCTGTAATTTTCTGTAACACTAAGCGGAAGGTTGAATGGTTAACTGAGAAGATGCGTAGTAATAACTTCACAGTTTCACATATGCATGGGGACATGCCTCAGAGGGAAAGAGATGCAATCATGGGGGAGTTCCGATCAGGAACTACTCGTGTCCTAATCACCACTGATGTTTGGGCACGAGGGCTTGATGTTCAGCAGGTATCACTAGTGATAAACTACGACCTTCCAAACAATCGAGAACTTTACATTCACAGGATAGGAAGATCTGGTCGGTTTGGGCGCAAGGGTGTTGCCATTAACTTTGTGAAAAGCGATGACATAAAGATCCTGAGAGATATTGAGCAGTATTACAGTACCCAGATTGATGAGATGCCCATGAACGTTGCTGATTTGATTTAA

Protein sequence

MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Homology
BLAST of Moc08g33770.1 vs. NCBI nr
Match: XP_022153139.1 (eukaryotic initiation factor 4A-3 [Momordica charantia])

HSP 1 Score: 807.0 bits (2083), Expect = 7.6e-230
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of Moc08g33770.1 vs. NCBI nr
Match: XP_038905027.1 (eukaryotic initiation factor 4A-3 [Benincasa hispida])

HSP 1 Score: 799.3 bits (2063), Expect = 1.6e-227
Identity = 404/410 (98.54%), Postives = 408/410 (99.51%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR++NAPEDKLEFETTEGVEPI +FDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRTVNAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI 180
           T KVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ+VSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TAKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of Moc08g33770.1 vs. NCBI nr
Match: XP_022949081.1 (eukaryotic initiation factor 4A-3-like [Cucurbita moschata] >XP_022998447.1 eukaryotic initiation factor 4A-3-like [Cucurbita maxima] >XP_023525647.1 eukaryotic initiation factor 4A-3-like [Cucurbita pepo subsp. pepo] >KAG6606907.1 hypothetical protein SDJN03_00249, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 798.5 bits (2061), Expect = 2.7e-227
Identity = 404/410 (98.54%), Postives = 408/410 (99.51%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR+I APEDKLEFETTEGVEPI SFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPII+GRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ+VSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQ+ERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of Moc08g33770.1 vs. NCBI nr
Match: XP_004148574.1 (eukaryotic initiation factor 4A-3 [Cucumis sativus] >KGN58518.1 hypothetical protein Csa_001953 [Cucumis sativus])

HSP 1 Score: 797.7 bits (2059), Expect = 4.6e-227
Identity = 403/410 (98.29%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR+I+APEDKLEFETTEGVEPI +FDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDT+SREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ+VSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQ+ERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of Moc08g33770.1 vs. NCBI nr
Match: XP_022983495.1 (eukaryotic initiation factor 4A-3 [Cucurbita maxima] >KAG7018051.1 hypothetical protein SDJN02_19917, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 796.6 bits (2056), Expect = 1.0e-226
Identity = 401/410 (97.80%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR+++APEDKLEFETTEGVEPI SFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAVSAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQ+VDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQVVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAH+CIGGKSVGEDIRKLEFGVQ+VSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHSCIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLL+LDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLILDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQ+ERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of Moc08g33770.1 vs. ExPASy Swiss-Prot
Match: P41380 (Eukaryotic initiation factor 4A-3 OS=Nicotiana plumbaginifolia OX=4092 PE=2 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 2.3e-205
Identity = 358/389 (92.03%), Postives = 374/389 (96.14%), Query Frame = 0

Query: 22  EDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQA 81
           ED+L FET++GVEPI+SF +MGIKDDLLRG+Y YGFEKPSAIQQRAV PII GRDVIAQA
Sbjct: 3   EDRLVFETSKGVEPIASFAEMGIKDDLLRGVYQYGFEKPSAIQQRAVLPIISGRDVIAQA 62

Query: 82  QSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIG 141
           QSGTGKTSMIALTVCQ+VDT S EVQALILSPTRELA QTEKVILAIGDYIN+QAHACIG
Sbjct: 63  QSGTGKTSMIALTVCQIVDTKSSEVQALILSPTRELAAQTEKVILAIGDYINVQAHACIG 122

Query: 142 GKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIY 201
           GKSVGEDIRKLE GVQ+VSGTPGRVCDMIKRRTLRTR IKLL+LDESDEMLSRGFKDQIY
Sbjct: 123 GKSVGEDIRKLEHGVQVVSGTPGRVCDMIKRRTLRTRGIKLLILDESDEMLSRGFKDQIY 182

Query: 202 DVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREE 261
           DVYRYLPPELQVVLISATLP+EILE+T+KFMTDPVRILVKRDELTLEGIKQFFVAVE+EE
Sbjct: 183 DVYRYLPPELQVVLISATLPNEILEITSKFMTDPVRILVKRDELTLEGIKQFFVAVEKEE 242

Query: 262 WKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQRERDAIMGE 321
           WKFDTLCDLYDTLTITQAVIFCNTKRKV+WLT KMR NNFTVS MHGDMPQ+ERDAIM E
Sbjct: 243 WKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTSKMRENNFTVSSMHGDMPQKERDAIMAE 302

Query: 322 FRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK 381
           FR GTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK
Sbjct: 303 FRGGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK 362

Query: 382 SDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           SDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 363 SDDIKILRDIEQYYSTQIDEMPMNVADLI 391

BLAST of Moc08g33770.1 vs. ExPASy Swiss-Prot
Match: Q5VNM3 (Eukaryotic initiation factor 4A-III homolog A OS=Oryza sativa subsp. japonica OX=39947 GN=EIF4A3A PE=1 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 1.3e-203
Identity = 361/409 (88.26%), Postives = 383/409 (93.64%), Query Frame = 0

Query: 3   AAATTSVVPPNRRRSINAPEDK-LEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKPS 62
           AAATTS      RR   A +D+ L FET+ GVE ISSFDQMGI+DDLLRGIYAYGFEKPS
Sbjct: 2   AAATTS------RRGPGAMDDENLTFETSPGVEVISSFDQMGIRDDLLRGIYAYGFEKPS 61

Query: 63  AIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQT 122
           AIQQRAV PII GRDVIAQAQSGTGKTSMI+L+VCQ+VDT+ REVQALILSPTRELA QT
Sbjct: 62  AIQQRAVLPIISGRDVIAQAQSGTGKTSMISLSVCQIVDTAVREVQALILSPTRELAAQT 121

Query: 123 EKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAIK 182
           E+V+LAIGD+INIQ HACIGGKS+GEDIRKLE GV +VSGTPGRVCDMIKRRTLRTRAIK
Sbjct: 122 ERVMLAIGDFINIQVHACIGGKSIGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIK 181

Query: 183 LLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVK 242
           LL+LDE+DEML RGFKDQIYDVYRYLPPELQV LISATLPHEILEMT+KFMTDPVRILVK
Sbjct: 182 LLILDEADEMLGRGFKDQIYDVYRYLPPELQVCLISATLPHEILEMTSKFMTDPVRILVK 241

Query: 243 RDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNF 302
           RDELTLEGIKQFFVAVE+EEWKFDTLCDLYDTLTITQAVIFCNTKRKV+WLTE+MRSNNF
Sbjct: 242 RDELTLEGIKQFFVAVEKEEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTERMRSNNF 301

Query: 303 TVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYI 362
           TVS MHGDMPQ+ERDAIMGEFRSG TRVLITTDVWARGLDVQQVSLVINYDLPNNRELYI
Sbjct: 302 TVSAMHGDMPQKERDAIMGEFRSGATRVLITTDVWARGLDVQQVSLVINYDLPNNRELYI 361

Query: 363 HRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           HRIGRSGRFGRKGVAINFVK +DI+ILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 362 HRIGRSGRFGRKGVAINFVKKEDIRILRDIEQYYSTQIDEMPMNVADLI 404

BLAST of Moc08g33770.1 vs. ExPASy Swiss-Prot
Match: Q10I26 (Eukaryotic initiation factor 4A-III homolog B OS=Oryza sativa subsp. japonica OX=39947 GN=EIF4A3B PE=1 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 1.3e-203
Identity = 361/409 (88.26%), Postives = 383/409 (93.64%), Query Frame = 0

Query: 3   AAATTSVVPPNRRRSINAPEDK-LEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKPS 62
           AAATTS      RR   A +D+ L FET+ GVE ISSFDQMGI++DLLRGIYAYGFEKPS
Sbjct: 2   AAATTS------RRGPGAMDDENLTFETSPGVEVISSFDQMGIREDLLRGIYAYGFEKPS 61

Query: 63  AIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQT 122
           AIQQRAV PII GRDVIAQAQSGTGKTSMI+L+VCQ+VDT+ REVQALILSPTRELA QT
Sbjct: 62  AIQQRAVLPIISGRDVIAQAQSGTGKTSMISLSVCQIVDTAVREVQALILSPTRELAAQT 121

Query: 123 EKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAIK 182
           E+V+LAIGDYINIQ HACIGGKS+GEDIRKLE GV +VSGTPGRVCDMIKRRTLRTRAIK
Sbjct: 122 ERVMLAIGDYINIQVHACIGGKSIGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIK 181

Query: 183 LLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVK 242
           LL+LDE+DEML RGFKDQIYDVYRYLPPELQV LISATLPHEILEMT+KFMTDPVRILVK
Sbjct: 182 LLILDEADEMLGRGFKDQIYDVYRYLPPELQVCLISATLPHEILEMTSKFMTDPVRILVK 241

Query: 243 RDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNF 302
           RDELTLEGIKQFFVAVE+EEWKFDTLCDLYDTLTITQAVIFCNTKRKV+WLTE+MRSNNF
Sbjct: 242 RDELTLEGIKQFFVAVEKEEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTERMRSNNF 301

Query: 303 TVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYI 362
           TVS MHGDMPQ+ERDAIMGEFRSG TRVLITTDVWARGLDVQQVSLVINYDLPNNRELYI
Sbjct: 302 TVSAMHGDMPQKERDAIMGEFRSGATRVLITTDVWARGLDVQQVSLVINYDLPNNRELYI 361

Query: 363 HRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           HRIGRSGRFGRKGVAINFVK +DI+ILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 362 HRIGRSGRFGRKGVAINFVKKEDIRILRDIEQYYSTQIDEMPMNVADLI 404

BLAST of Moc08g33770.1 vs. ExPASy Swiss-Prot
Match: Q94A52 (Eukaryotic initiation factor 4A-III homolog OS=Arabidopsis thaliana OX=3702 GN=EIF4A3 PE=1 SV=2)

HSP 1 Score: 696.4 bits (1796), Expect = 1.9e-199
Identity = 345/389 (88.69%), Postives = 374/389 (96.14%), Query Frame = 0

Query: 22  EDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQA 81
           +DKL FETT+G+EPI+SF+ MGIK+D+LRG+Y YGFEKPSAIQQRAV PI++GRDVIAQA
Sbjct: 20  DDKLVFETTDGIEPITSFNDMGIKEDVLRGVYEYGFEKPSAIQQRAVMPILQGRDVIAQA 79

Query: 82  QSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIG 141
           QSGTGKTSMIAL+VCQ+VDTSSREVQALILSPTRELATQTEK I AIG + NIQAHACIG
Sbjct: 80  QSGTGKTSMIALSVCQVVDTSSREVQALILSPTRELATQTEKTIQAIGLHANIQAHACIG 139

Query: 142 GKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIY 201
           G SVGEDIRKLE GV +VSGTPGRVCDMIKRR+LRTRAIKLL+LDESDEMLSRGFKDQIY
Sbjct: 140 GNSVGEDIRKLEHGVHVVSGTPGRVCDMIKRRSLRTRAIKLLILDESDEMLSRGFKDQIY 199

Query: 202 DVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREE 261
           DVYRYLPP+LQV L+SATLPHEILEMT+KFMT+PV+ILVKRDELTLEGIKQFFVAVE+EE
Sbjct: 200 DVYRYLPPDLQVCLVSATLPHEILEMTSKFMTEPVKILVKRDELTLEGIKQFFVAVEKEE 259

Query: 262 WKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQRERDAIMGE 321
           WKFDTLCDLYDTLTITQAVIFCNTKRKV++L+EKMRS+NFTVS MHGDMPQ+ERDAIM E
Sbjct: 260 WKFDTLCDLYDTLTITQAVIFCNTKRKVDYLSEKMRSHNFTVSSMHGDMPQKERDAIMNE 319

Query: 322 FRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK 381
           FRSG +RVLITTDVWARG+DVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK
Sbjct: 320 FRSGDSRVLITTDVWARGIDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK 379

Query: 382 SDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           SDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 380 SDDIKILRDIEQYYSTQIDEMPMNVADLI 408

BLAST of Moc08g33770.1 vs. ExPASy Swiss-Prot
Match: Q7ZVA6 (Eukaryotic initiation factor 4A-III OS=Danio rerio OX=7955 GN=eif4a3 PE=2 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 1.7e-184
Identity = 317/404 (78.47%), Postives = 363/404 (89.85%), Query Frame = 0

Query: 7   TSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKPSAIQQR 66
           T+VVP  +R        K+EFET+E V+   +FD MG+++DLLRGIYAYGFEKPSAIQQR
Sbjct: 3   TAVVPVRKRILKEEDMTKIEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQR 62

Query: 67  AVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQTEKVIL 126
           A++ II+GRDVIAQ+QSGTGKT+   ++V Q +D   RE QALIL+PTRELA Q +KV+L
Sbjct: 63  AIKQIIKGRDVIAQSQSGTGKTATFCVSVLQCLDIQVRETQALILAPTRELAGQIQKVLL 122

Query: 127 AIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAIKLLVLD 186
           A+GDY+N+Q HACIGG +VGEDIRKL++G  +V+GTPGRV DMI+RR+LRTRAIK+LVLD
Sbjct: 123 ALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLD 182

Query: 187 ESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELT 246
           E+DEML++GFK+QIYDVYRYLPP  QV LISATLPHEILEMTNKFMTDP+RILVKRDELT
Sbjct: 183 EADEMLNKGFKEQIYDVYRYLPPATQVCLISATLPHEILEMTNKFMTDPIRILVKRDELT 242

Query: 247 LEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHM 306
           LEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKV+WLTEKMR  NFTVS M
Sbjct: 243 LEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSM 302

Query: 307 HGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGR 366
           HGDMPQ+ER++IM EFRSG +RVLI+TDVWARGLDV QVSL+INYDLPNNRELYIHRIGR
Sbjct: 303 HGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVSQVSLIINYDLPNNRELYIHRIGR 362

Query: 367 SGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           SGR+GRKGVAINFVK+DDI+ILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 363 SGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI 406

BLAST of Moc08g33770.1 vs. ExPASy TrEMBL
Match: A0A6J1DI60 (eukaryotic initiation factor 4A-3 OS=Momordica charantia OX=3673 GN=LOC111020708 PE=4 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 3.7e-230
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of Moc08g33770.1 vs. ExPASy TrEMBL
Match: A0A6J1K814 (eukaryotic initiation factor 4A-3-like OS=Cucurbita maxima OX=3661 GN=LOC111493076 PE=4 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.3e-227
Identity = 404/410 (98.54%), Postives = 408/410 (99.51%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR+I APEDKLEFETTEGVEPI SFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPII+GRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ+VSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQ+ERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of Moc08g33770.1 vs. ExPASy TrEMBL
Match: A0A6J1GB02 (eukaryotic initiation factor 4A-3-like OS=Cucurbita moschata OX=3662 GN=LOC111452540 PE=4 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.3e-227
Identity = 404/410 (98.54%), Postives = 408/410 (99.51%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR+I APEDKLEFETTEGVEPI SFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPII+GRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ+VSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQ+ERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of Moc08g33770.1 vs. ExPASy TrEMBL
Match: A0A0A0L9P0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G654480 PE=4 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.2e-227
Identity = 403/410 (98.29%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR+I+APEDKLEFETTEGVEPI +FDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDT+SREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ+VSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQ+ERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of Moc08g33770.1 vs. ExPASy TrEMBL
Match: A0A6J1J605 (eukaryotic initiation factor 4A-3 OS=Cucurbita maxima OX=3661 GN=LOC111482085 PE=4 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 5.0e-227
Identity = 401/410 (97.80%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRSINAPEDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR+++APEDKLEFETTEGVEPI SFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAVSAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQ+VDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQVVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAH+CIGGKSVGEDIRKLEFGVQ+VSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHSCIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLL+LDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLILDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360
           FTVSHMHGDMPQ+ERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of Moc08g33770.1 vs. TAIR 10
Match: AT3G19760.1 (eukaryotic initiation factor 4A-III )

HSP 1 Score: 696.4 bits (1796), Expect = 1.3e-200
Identity = 345/389 (88.69%), Postives = 374/389 (96.14%), Query Frame = 0

Query: 22  EDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQA 81
           +DKL FETT+G+EPI+SF+ MGIK+D+LRG+Y YGFEKPSAIQQRAV PI++GRDVIAQA
Sbjct: 20  DDKLVFETTDGIEPITSFNDMGIKEDVLRGVYEYGFEKPSAIQQRAVMPILQGRDVIAQA 79

Query: 82  QSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIG 141
           QSGTGKTSMIAL+VCQ+VDTSSREVQALILSPTRELATQTEK I AIG + NIQAHACIG
Sbjct: 80  QSGTGKTSMIALSVCQVVDTSSREVQALILSPTRELATQTEKTIQAIGLHANIQAHACIG 139

Query: 142 GKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIY 201
           G SVGEDIRKLE GV +VSGTPGRVCDMIKRR+LRTRAIKLL+LDESDEMLSRGFKDQIY
Sbjct: 140 GNSVGEDIRKLEHGVHVVSGTPGRVCDMIKRRSLRTRAIKLLILDESDEMLSRGFKDQIY 199

Query: 202 DVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREE 261
           DVYRYLPP+LQV L+SATLPHEILEMT+KFMT+PV+ILVKRDELTLEGIKQFFVAVE+EE
Sbjct: 200 DVYRYLPPDLQVCLVSATLPHEILEMTSKFMTEPVKILVKRDELTLEGIKQFFVAVEKEE 259

Query: 262 WKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQRERDAIMGE 321
           WKFDTLCDLYDTLTITQAVIFCNTKRKV++L+EKMRS+NFTVS MHGDMPQ+ERDAIM E
Sbjct: 260 WKFDTLCDLYDTLTITQAVIFCNTKRKVDYLSEKMRSHNFTVSSMHGDMPQKERDAIMNE 319

Query: 322 FRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK 381
           FRSG +RVLITTDVWARG+DVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK
Sbjct: 320 FRSGDSRVLITTDVWARGIDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK 379

Query: 382 SDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           SDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 380 SDDIKILRDIEQYYSTQIDEMPMNVADLI 408

BLAST of Moc08g33770.1 vs. TAIR 10
Match: AT1G51380.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 576.6 bits (1485), Expect = 1.6e-164
Identity = 286/389 (73.52%), Postives = 339/389 (87.15%), Query Frame = 0

Query: 22  EDKLEFETTEGVEPISSFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQA 81
           E+ L FETT+G++PI SFD MG+ D +LRG+Y YG++KPS IQQRA+ PI++GRDVIAQA
Sbjct: 7   EENLVFETTKGIKPIKSFDDMGMNDKVLRGVYDYGYKKPSEIQQRALVPILKGRDVIAQA 66

Query: 82  QSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIG 141
           QSGTGKTSMIA++VCQ+V+ SSR+VQ L+LSP+RELA+QTEK I AIG + NIQAHACIG
Sbjct: 67  QSGTGKTSMIAISVCQIVNISSRKVQVLVLSPSRELASQTEKTIQAIGAHTNIQAHACIG 126

Query: 142 GKSVGEDIRKLEFGVQIVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIY 201
           GKS+GEDI+KLE GV  VSGTPGRV DMIKR +L+T+A+KLLVLDESDEMLS+G KDQIY
Sbjct: 127 GKSIGEDIKKLERGVHAVSGTPGRVYDMIKRGSLQTKAVKLLVLDESDEMLSKGLKDQIY 186

Query: 202 DVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREE 261
           DVYR LP ++QV LISATLP EILEMT KFMTDPVRILVK DELTLEGIKQ++V V++EE
Sbjct: 187 DVYRALPHDIQVCLISATLPQEILEMTEKFMTDPVRILVKPDELTLEGIKQYYVDVDKEE 246

Query: 262 WKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQRERDAIMGE 321
           WKFDTLCDLY  LTI QA+IFCNT++KV+WLTEKMRS+NF VS MHGD  Q+ERD IM +
Sbjct: 247 WKFDTLCDLYGRLTINQAIIFCNTRQKVDWLTEKMRSSNFIVSSMHGDKRQKERDDIMNQ 306

Query: 322 FRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK 381
           FRS  +RVLI +DVWARG+DVQ VS VINYD+PNN ELYIHRIGR+GRFGR+GVAINFVK
Sbjct: 307 FRSFKSRVLIASDVWARGIDVQTVSHVINYDIPNNPELYIHRIGRAGRFGREGVAINFVK 366

Query: 382 SDDIKILRDIEQYYSTQIDEMPMNVADLI 411
           S D+K L+DIE++Y T+I EMP   ADL+
Sbjct: 367 SSDMKDLKDIERHYGTKIREMP---ADLV 392

BLAST of Moc08g33770.1 vs. TAIR 10
Match: AT3G13920.1 (eukaryotic translation initiation factor 4A1 )

HSP 1 Score: 503.4 bits (1295), Expect = 1.7e-142
Identity = 248/373 (66.49%), Postives = 306/373 (82.04%), Query Frame = 0

Query: 38  SFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQ 97
           SFD MG++++LLRGIYAYGFEKPSAIQQR + P  +G DVI QAQSGTGKT+     V Q
Sbjct: 40  SFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGVLQ 99

Query: 98  MVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ 157
            +D S  + QAL+L+PTRELA Q EKV+ A+GDY+ ++ HAC+GG SV ED R L+ GV 
Sbjct: 100 QLDFSLIQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVH 159

Query: 158 IVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLIS 217
           +V GTPGRV DM+KR++LR   IK+ VLDE+DEMLSRGFKDQIYD+++ LPP++QV + S
Sbjct: 160 VVVGTPGRVFDMLKRQSLRADNIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFS 219

Query: 218 ATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTIT 277
           AT+P E LE+T KFM+ PVRILVKRDELTLEGIKQF+V VE+EEWK +TLCDLY+TL IT
Sbjct: 220 ATMPPEALEITRKFMSKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAIT 279

Query: 278 QAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWA 337
           Q+VIF NT+RKV+WLT+KMRS + TVS  HGDM Q  RD IM EFRSG++RVLITTD+ A
Sbjct: 280 QSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLA 339

Query: 338 RGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYST 397
           RG+DVQQVSLVIN+DLP   E Y+HRIGRSGRFGRKGVAINFV  DD ++L DI+++Y+ 
Sbjct: 340 RGIDVQQVSLVINFDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNV 399

Query: 398 QIDEMPMNVADLI 411
            ++E+P NVADL+
Sbjct: 400 VVEELPSNVADLL 412

BLAST of Moc08g33770.1 vs. TAIR 10
Match: AT3G13920.3 (eukaryotic translation initiation factor 4A1 )

HSP 1 Score: 503.4 bits (1295), Expect = 1.7e-142
Identity = 248/373 (66.49%), Postives = 306/373 (82.04%), Query Frame = 0

Query: 38  SFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQ 97
           SFD MG++++LLRGIYAYGFEKPSAIQQR + P  +G DVI QAQSGTGKT+     V Q
Sbjct: 30  SFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGVLQ 89

Query: 98  MVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ 157
            +D S  + QAL+L+PTRELA Q EKV+ A+GDY+ ++ HAC+GG SV ED R L+ GV 
Sbjct: 90  QLDFSLIQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVH 149

Query: 158 IVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLIS 217
           +V GTPGRV DM+KR++LR   IK+ VLDE+DEMLSRGFKDQIYD+++ LPP++QV + S
Sbjct: 150 VVVGTPGRVFDMLKRQSLRADNIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFS 209

Query: 218 ATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTIT 277
           AT+P E LE+T KFM+ PVRILVKRDELTLEGIKQF+V VE+EEWK +TLCDLY+TL IT
Sbjct: 210 ATMPPEALEITRKFMSKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAIT 269

Query: 278 QAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWA 337
           Q+VIF NT+RKV+WLT+KMRS + TVS  HGDM Q  RD IM EFRSG++RVLITTD+ A
Sbjct: 270 QSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLA 329

Query: 338 RGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYST 397
           RG+DVQQVSLVIN+DLP   E Y+HRIGRSGRFGRKGVAINFV  DD ++L DI+++Y+ 
Sbjct: 330 RGIDVQQVSLVINFDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNV 389

Query: 398 QIDEMPMNVADLI 411
            ++E+P NVADL+
Sbjct: 390 VVEELPSNVADLL 402

BLAST of Moc08g33770.1 vs. TAIR 10
Match: AT1G54270.1 (eif4a-2 )

HSP 1 Score: 498.4 bits (1282), Expect = 5.4e-141
Identity = 245/373 (65.68%), Postives = 305/373 (81.77%), Query Frame = 0

Query: 38  SFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQ 97
           SFD MG++++LLRGIYAYGFEKPSAIQQR + P  +G DVI QAQSGTGKT+     V Q
Sbjct: 40  SFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGVLQ 99

Query: 98  MVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ 157
            +D +  + QAL+L+PTRELA Q EKV+ A+GDY  ++ HAC+GG SV ED R L+ GV 
Sbjct: 100 QLDYALLQCQALVLAPTRELAQQIEKVMRALGDYQGVKVHACVGGTSVREDQRILQAGVH 159

Query: 158 IVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLIS 217
           +V GTPGRV DM++R++LR   IK+ VLDE+DEMLSRGFKDQIYD+++ LPP++QV + S
Sbjct: 160 VVVGTPGRVFDMLRRQSLRPDCIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFS 219

Query: 218 ATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTIT 277
           AT+P E LE+T KFM+ PVRILVKRDELTLEGIKQF+V VE+E+WK +TLCDLY+TL IT
Sbjct: 220 ATMPPEALEITRKFMSKPVRILVKRDELTLEGIKQFYVNVEKEDWKLETLCDLYETLAIT 279

Query: 278 QAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQRERDAIMGEFRSGTTRVLITTDVWA 337
           Q+VIF NT+RKV+WLT+KMRS + TVS  HGDM Q  RD IM EFRSG++RVLITTD+ A
Sbjct: 280 QSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLA 339

Query: 338 RGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYST 397
           RG+DVQQVSLVIN+DLP   E Y+HRIGRSGRFGRKGVAINFV  DD ++L DI+++Y+ 
Sbjct: 340 RGIDVQQVSLVINFDLPTQPENYLHRIGRSGRFGRKGVAINFVTLDDQRMLFDIQKFYNV 399

Query: 398 QIDEMPMNVADLI 411
            ++E+P NVADL+
Sbjct: 400 VVEELPSNVADLL 412

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153139.17.6e-230100.00eukaryotic initiation factor 4A-3 [Momordica charantia][more]
XP_038905027.11.6e-22798.54eukaryotic initiation factor 4A-3 [Benincasa hispida][more]
XP_022949081.12.7e-22798.54eukaryotic initiation factor 4A-3-like [Cucurbita moschata] >XP_022998447.1 euka... [more]
XP_004148574.14.6e-22798.29eukaryotic initiation factor 4A-3 [Cucumis sativus] >KGN58518.1 hypothetical pro... [more]
XP_022983495.11.0e-22697.80eukaryotic initiation factor 4A-3 [Cucurbita maxima] >KAG7018051.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
P413802.3e-20592.03Eukaryotic initiation factor 4A-3 OS=Nicotiana plumbaginifolia OX=4092 PE=2 SV=1[more]
Q5VNM31.3e-20388.26Eukaryotic initiation factor 4A-III homolog A OS=Oryza sativa subsp. japonica OX... [more]
Q10I261.3e-20388.26Eukaryotic initiation factor 4A-III homolog B OS=Oryza sativa subsp. japonica OX... [more]
Q94A521.9e-19988.69Eukaryotic initiation factor 4A-III homolog OS=Arabidopsis thaliana OX=3702 GN=E... [more]
Q7ZVA61.7e-18478.47Eukaryotic initiation factor 4A-III OS=Danio rerio OX=7955 GN=eif4a3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DI603.7e-230100.00eukaryotic initiation factor 4A-3 OS=Momordica charantia OX=3673 GN=LOC111020708... [more]
A0A6J1K8141.3e-22798.54eukaryotic initiation factor 4A-3-like OS=Cucurbita maxima OX=3661 GN=LOC1114930... [more]
A0A6J1GB021.3e-22798.54eukaryotic initiation factor 4A-3-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A0A0L9P02.2e-22798.29Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G654480 PE=4 SV=1[more]
A0A6J1J6055.0e-22797.80eukaryotic initiation factor 4A-3 OS=Cucurbita maxima OX=3661 GN=LOC111482085 PE... [more]
Match NameE-valueIdentityDescription
AT3G19760.11.3e-20088.69eukaryotic initiation factor 4A-III [more]
AT1G51380.11.6e-16473.52DEA(D/H)-box RNA helicase family protein [more]
AT3G13920.11.7e-14266.49eukaryotic translation initiation factor 4A1 [more]
AT3G13920.31.7e-14266.49eukaryotic translation initiation factor 4A1 [more]
AT1G54270.15.4e-14165.68eif4a-2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 290..371
e-value: 1.4E-33
score: 127.5
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 263..371
e-value: 6.7E-31
score: 106.9
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 249..410
score: 26.796547
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 56..253
e-value: 2.7E-52
score: 189.7
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 68..238
score: 25.561119
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 27..245
e-value: 7.5E-65
score: 220.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 246..410
e-value: 7.6E-60
score: 202.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 105..393
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 62..224
e-value: 1.6E-41
score: 142.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR47958:SF55BNAC03G41130D PROTEINcoord: 22..403
NoneNo IPR availablePANTHERPTHR47958ATP-DEPENDENT RNA HELICASE DBP3coord: 22..403
NoneNo IPR availableCDDcd18045DEADc_EIF4AIII_DDX48coord: 39..239
e-value: 6.54573E-123
score: 352.54
NoneNo IPR availableCDDcd18787SF2_C_DEADcoord: 250..380
e-value: 1.9819E-62
score: 195.419
IPR014014RNA helicase, DEAD-box type, Q motifPROSITEPS51195Q_MOTIFcoord: 37..65
score: 10.976758

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Moc08g33770Moc08g33770gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Moc08g33770.1.exon1Moc08g33770.1.exon1exon
Moc08g33770.1.exon2Moc08g33770.1.exon2exon
Moc08g33770.1.exon3Moc08g33770.1.exon3exon
Moc08g33770.1.exon4Moc08g33770.1.exon4exon
Moc08g33770.1.exon5Moc08g33770.1.exon5exon
Moc08g33770.1.exon6Moc08g33770.1.exon6exon
Moc08g33770.1.exon7Moc08g33770.1.exon7exon
Moc08g33770.1.exon8Moc08g33770.1.exon8exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Moc08g33770.1cds.Moc08g33770.1CDS
cds.Moc08g33770.1cds.Moc08g33770.1_2CDS
cds.Moc08g33770.1cds.Moc08g33770.1_3CDS
cds.Moc08g33770.1cds.Moc08g33770.1_4CDS
cds.Moc08g33770.1cds.Moc08g33770.1_5CDS
cds.Moc08g33770.1cds.Moc08g33770.1_6CDS
cds.Moc08g33770.1cds.Moc08g33770.1_7CDS
cds.Moc08g33770.1cds.Moc08g33770.1_8CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Moc08g33770.1Moc08g33770.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006413 translational initiation
cellular_component GO:0071013 catalytic step 2 spliceosome
cellular_component GO:0005730 nucleolus
molecular_function GO:0005524 ATP binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003724 RNA helicase activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003743 translation initiation factor activity