Moc08g22460.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g22460.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease T(2)
Location	chr8: 16464641 .. 16470224 (-)
Sequence length	654
RNA-Seq Expression	Moc08g22460.1
Synteny	Moc08g22460.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAAGACAGAAAGTGGAGTGTTGATGTTGGGCTTAGTTGTTGCAGTGATAATTTGTGAGGCTGCTGCTTACGATTACTTTCAGATGGTGGAACAGTGGCAGCCTGCAACCTGCAGCGACAAGAAGCTGAGATTTGGATGCAGGCAGAAGCCGAAGCAAGTGTTTACTATCCATGGCTTGTGGCCAAGTAACTATTCTAGCGCAAGGTCCCAACCCTGCAGGGGCACTAGATTTGATGTCACCCAGGTATTACTTCTTTTTCTTTATTGTTTATTGTTCCCGTTTTCTGTTTCTGGTTTGTTGTTGTTAAAAGGATCCCACATTGTCAATTGGTTTTGAGATTGAACCCATGAATCTTGATATGGTATTAGAACTCAAAATGTTCAAACCAGCAATCGGTCCAAAAATGGGATCCGGCCAAGATCGATGAGTCAAAAACAGCCACGATCTGAGAGGGCATGTTTAGGAACCCAAATTGAAAAGAAAATTGACCTTACAATGGTTTATAATAGACATGGGTTACTCATCTAATTTTCAATTGGTTTTGAGATGAAACCCATGAATATTGATATGGTATCAGAGCCCAAAAACGCTCAAACGAGCATCCGGTCCAAGATCCGGCCAAGATCGATGAGCCAAAAAGAGTCACGATCTTGAGGGGCATGTTCGGTTTATAATAGACATGAGTTACTCCTCTCATTACGAATTGGTTCTAGATGGAACACTTTGTTAGAAGTAGAATATTTCCGTGTTCTTGTTTTTGTTTTTAGTATTTTTAAGTACAAAAACTTTTGAGATGAGGATTGAAACCACGACTTCTTGATTAGAGATATATGTCTTAACTTAGATTATTTTATTATTATTATTATTTTTAGTATTTTTAGTACAACATACTAGGGTGGACATTCAAATAGTCGGACATAGTATCTAAATCAAGTTGAGCTATACTCGAATTGACTATTTTTATTTGAAAAAAAAGAATTAAATATAAAACAAGAGATAATGTTTGTCGACACATGAGACTCAAACATAGACATGTAAGTTGAACATATTCTGCCTACATCAATAGAGCTGAGAGTACACATTGGATATTGGAGGCATTTAACCTAAAACCGAGAAACATATAAACAAAAACTATTTTTTATAGTTTCCAAATAATCGAAGTCCTTTTTTGAAAACATTTCTGAAATATTAGAAAGGATAAACAAAAAATAATAAACAGGAACGTTGTCAAATGGACACTTAATTTCTATATCTTAAAGAGTATCGAGTAGGTCGAAAACTCTACAAGATGTGGCTATCTAGCAATAAACAAGAACGTTGTCAAATGAACCTTTAATTTCTATATTTTAAAGAGCATCGAGTAGGCCGAAAACTCTACCAGATGTGGCTATCTAGTTTTTGTACTTTAATAAGTGTCTGAGAATTTGAATAGGTGGTTGAAATTTCAATATTTTTTGAACTAATAAAGATAAAAATAATGCTTAATTAATAGGTTCAATGTCGTTTTGTAACTAGTAAACACAAATAATTCCTTTGCAAAAAAGACAAAAAATAATACTTAATTAATTAAGGTAACGACAGGTATAGCCATTTAAAATAGGTGATTTCAAAAAAAAAGCCGAACAAGTTCCACTTTTGAAAAATTACCAATTTAAAAATACGAAAATCTCTAGTAATATTTAGGTATGAAATATCCCTTTCATCATTTTTAAATCAATATATTATTTATCCTTTTTTATAGGAGAATCATACTTATATTTTTTTCTAATGAATCATTCAAATATTTTGAAAATCTTACTTATATTTTTTAATTCAAATATTTTTCCAAGACAATTTTACTAACAAACCTAAACGGAAAAAAATATTAATTAAATTAAAATCTAAAACAAAATTTAAACAAAAAAAACATTTTAGAAATAGAAATACCAAATAAATTTAAATAACAAAATCATTTTAGAAATGGAAATACCAATGCCGAAGGTGGAAACAAATACAGTAACAAACATGATGAACAAATATAAATAAATACAGTAACAAGCTAGCATGATGAACAAATACGGTGAACAAATTAACAAATATAGTGAACAAATGAACAGATATGGTGAACATATAACAAATACGATGAATAGATGAACAAATACCTGGACTTTAAAATGAACATATATTGTGAAAAATAGGGTGAACACATAATGTTTTTTTTTACACAATTAACACAAAAATATAAAAAAGAATCTTCCTAAAAAGGTACCGTAAAAACTCATACTATTGACCTCTCTCTTCTTTACATCCGTCACTCCCCACCCCAGTTATAGCTTTGTCACCCGCCTTGTTGCTCCCCCATTGCATCCCCTTTCTCCCTTGCTCAATTCCATCCACTCCCCGCCCCACCTACAACTATGTCACTCGCTTTGTTGCTCCACCGTTGTCTCCCTTGCTCAATCCCGTCCATTCCCTGCCTTGTTATCATCCGGCCCTTAGCTCAATTTCTCGTCCATTCCCGTTATCTCCCTCGCGATATCCATTGATCATGTCTCACCTTCTGTGTTGTCCTCTTTGAGTTATCTTATGTTTTATTCAAATATATATTTAAAATGTTAGATATATGATTTCTATAATATAGGAGATCAAATGTAGGAGAATAGACATAATATAACAAGAAACAAATTTGAGAATCTATGAGGGGAGTATTATTTTGATTTTGAAAATACTAACTAACTAGAAAGGGTAAGATGGTCAAAATTTTTACCGTTTGAAAAATCGAAACGAACATTTTATTAACAAAAACATCTAGCCATTTTTCAAAACAAGTGACAAATAGCGACACCTATCCTAAATTCTCTAATTATAATATTAGATATTTTTTAAAAAAATTTAGGTCCTATTAGAATTTTTTTAAATATAGCAGAGTTTAGAGACCTAGCTATTAAATTTGTTTCGAGTATAATGATTAAACAAAATAGTTTCATATATAAATTGAGTGATCAAACTTGTTCTATATCCTCCAGCTTTTGTTTGATTTTATGGAGATTTAAAAATGTTCTATATATATTTATCGTTAGAAATAATCAACTTGTCCATTTGGTAACGTTTTTGTTTATTGTTTTCTGTTTTTTTAATCAAGAAAAACATATATAATTATATTTGGTAACTATTTTTTGTTTTTTACGGATTATAAGAATATATATATATAGATATAATTTATGTTTTGGAAAAAAAAAATGAAACAGAACAAGAAACAATAAACTTGATTTTGTTGCCTCCAAGATTTCTATATAATTTTAGAAACATTTTTCAAAAATAAAAAACAAGATAAATAAATAGTTGTCAAATACGTAGAATTTTCAATAAAATTGGAAAAAAATAACATGGGAACATTAACGAATTTACCCTCAGCGATCTAGCGATTTAATAATCTATGAAGTTTGATTATTGTGTATATTTATTATCATTTCAAAATTCAATTATATTTTTGAACTTTTAATTTTGATATATAAAGTAACCTAACGCAATTAAAATACCACGCACAAAAAAGGTATACCACGGGACACGGGGATAAAATAAAGTGAGATTAATGAATAAATTTCACTTAAAATTCTATTAGTTTTTGTTCATTCTAATTTTATCGAGTTGACCCGCTGATTATTTTACTGTTTAAAAAGAGTTTATGAACTATTTAGACTATAGTTATAGTTAATATATATTCAATTGTTTTATTATTGATCGGTATAAGTGCAATTAAATTTTTTTATTATTTATTATTTTTTTCATGTTAAATATTTTTTATGTCATAAATTTAACTTCATAAATGTATATCAATTGTTTGGATTGCATTTTTATCTTAACCTAACATATAAAGAATAAAAGTCATTCTACTCTTATTCAAATATAAATAATCTACCTTCCATTACGAATTTATGTTACATTAAAATACATGTTAATTTTTTACCACACATATATATATATATATATATATATATTTTAGTACACATGTGGGAGGAATTTCAAACTAACAACTTCTTAATTAGAGGTAGGTGTCAATTACCGATCAATACTCGTTTTAGTCATTCACATTAAATTTAAAAAGAAAATAATCTAAACATAATTAAGAAAAAGGGAAAAAATAGGATCCGCATTGCACATATAAATGCCTTATTATCGGGTAAGAAATTTCAAATACCCACAAAATAGTTTCCTACTCGAGGCATAATTCTTTGAAGATTTTCTCTAGCCAAAGTCGTCATTTTCTTTTTCTTTTTTGGAAAAGAAAAAACTTAATCAGTAAAAGATTTTAATATTTAAAATTTATAATAAAATTGGATTAAAGAGTGAACCTTTTTTTTTTCTCCCACCACCATATTTCTGTTGAGATTAAATTATAATTATGTATTTCGAAATGTGTCACCTGTTTTTCAAATTTTCAAATATAACATAAATTTTCACTTATATTAATAGTATATGAGCTGATACTCCTATTAATAGTATATTAGTAGTATATCAGACACTCATATCACTAGTATATCAGATGTATATCACATACATATTGCACAAATATTCATATTAGTAGTATATTAGATTTATATCACATACATATTGCAAATATATCAGAGACTCATATCAGTAATATATCAGATGTATATATCACGCATATATATAAGTAGTCAGATATATTACAGATGTATATCATTTTGGTAAAGATGTCATTTTAAAATTGGACTTTCGTTTTTTATTTTAAAAAAAATGCTATACTTGAAAAACTAAGTGTAGTTTCTATTTTTCTAATTTTACCAAATATATTTTTGCCATATTTACAACTAACCCTAGAATTTATCTAACCAGCATACTAAGTATTTCATTGATGTAGCCAACCATTGGGTCTAAATATATAAACATAACAGTAATTTTAATGATACAATATAATTAAAACATTTCAAAGTTTGAAAATGCTAGCCAACATGAGTTTTAGGTAAGTAGTACGTGTCTCTGACTAAAAGGTCAAGAGCTCAAATCTCCAATCCCAAATATTGTACTAAAAAAAGGTTGAAAATGCTAAATAAACACAATATTTATAAATAAATACAATGTTCAAATTTTAGATGCTAAATTTATTTAACATTGGTTTAATGTTTGAAAAATTCATATAAAAAATTGTACTGTTTTTCATCAGATCGCTCCCATAAGAGCACAACTAAACCAATATTGGCCGGACGTGGTGAATGGAAACAACCAGAGGTTTTGGGAGCATGAGTGGTCAAAGCATGGGACCTGTTCCGAGAAGCAATTCCCAGAAAGAGCTTATTTCCAAAACGCTTTAGATATTGCAAAAAGATATGATCTACTGTCCATCCTCAAGAGGGCAGGCCAAAACCCAGATGGAAAAGTCAAGCAAAGAGCCAACATTGAAGCTGCCATTAGTAGAGCCATTGGAAAACGGCCTGTGCTTCGCTGCAATTTCAACAAGTCCAATGCTAGACAGCTGCACGAGATCGTCCTATGTGTCGCTAAGAACGGTGTCGCACTCGAGAGCTGTACCAAGTTTGCTGCTGCCACATGCCCAAACACTTTTGTGTGGGGTAGTTGA

mRNA sequence

ATGGGAAAGACAGAAAGTGGAGTGTTGATGTTGGGCTTAGTTGTTGCAGTGATAATTTGTGAGGCTGCTGCTTACGATTACTTTCAGATGGTGGAACAGTGGCAGCCTGCAACCTGCAGCGACAAGAAGCTGAGATTTGGATGCAGGCAGAAGCCGAAGCAAGTGTTTACTATCCATGGCTTGTGGCCAAGTAACTATTCTAGCGCAAGGTCCCAACCCTGCAGGGGCACTAGATTTGATATCGCTCCCATAAGAGCACAACTAAACCAATATTGGCCGGACGTGGTGAATGGAAACAACCAGAGGTTTTGGGAGCATGAGTGGTCAAAGCATGGGACCTGTTCCGAGAAGCAATTCCCAGAAAGAGCTTATTTCCAAAACGCTTTAGATATTGCAAAAAGATATGATCTACTGTCCATCCTCAAGAGGGCAGGCCAAAACCCAGATGGAAAAGTCAAGCAAAGAGCCAACATTGAAGCTGCCATTAGTAGAGCCATTGGAAAACGGCCTGTGCTTCGCTGCAATTTCAACAAGTCCAATGCTAGACAGCTGCACGAGATCGTCCTATGTGTCGCTAAGAACGGTGTCGCACTCGAGAGCTGTACCAAGTTTGCTGCTGCCACATGCCCAAACACTTTTGTGTGGGGTAGTTGA

Coding sequence (CDS)

ATGGGAAAGACAGAAAGTGGAGTGTTGATGTTGGGCTTAGTTGTTGCAGTGATAATTTGTGAGGCTGCTGCTTACGATTACTTTCAGATGGTGGAACAGTGGCAGCCTGCAACCTGCAGCGACAAGAAGCTGAGATTTGGATGCAGGCAGAAGCCGAAGCAAGTGTTTACTATCCATGGCTTGTGGCCAAGTAACTATTCTAGCGCAAGGTCCCAACCCTGCAGGGGCACTAGATTTGATATCGCTCCCATAAGAGCACAACTAAACCAATATTGGCCGGACGTGGTGAATGGAAACAACCAGAGGTTTTGGGAGCATGAGTGGTCAAAGCATGGGACCTGTTCCGAGAAGCAATTCCCAGAAAGAGCTTATTTCCAAAACGCTTTAGATATTGCAAAAAGATATGATCTACTGTCCATCCTCAAGAGGGCAGGCCAAAACCCAGATGGAAAAGTCAAGCAAAGAGCCAACATTGAAGCTGCCATTAGTAGAGCCATTGGAAAACGGCCTGTGCTTCGCTGCAATTTCAACAAGTCCAATGCTAGACAGCTGCACGAGATCGTCCTATGTGTCGCTAAGAACGGTGTCGCACTCGAGAGCTGTACCAAGTTTGCTGCTGCCACATGCCCAAACACTTTTGTGTGGGGTAGTTGA

Protein sequence

MGKTESGVLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHGLWPSNYSSARSQPCRGTRFDIAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEKQFPERAYFQNALDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFNKSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVWGS

Homology

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BLAST of Moc08g22460.1 vs. NCBI nr
Match: XP_022159207.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 450.3 bits (1157), Expect = 9.5e-123
Identity = 217/220 (98.64%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 1   MGKTESGVLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHG 60
           MGKTESGVLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHG
Sbjct: 1   MGKTESGVLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHG 60

Query: 61  LWPSNYSSARSQPCRGTRFD---IAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEK 120
           LWPSNYSSARSQPCRGTRFD   IAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEK
Sbjct: 61  LWPSNYSSARSQPCRGTRFDVTQIAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEK 120

Query: 121 QFPERAYFQNALDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFN 180
           QFPERAYFQNALDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFN
Sbjct: 121 QFPERAYFQNALDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFN 180

Query: 181 KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVWGS 218
           KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVWGS
Sbjct: 181 KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVWGS 220

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BLAST of Moc08g22460.1 vs. NCBI nr
Match: XP_022158938.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 422.2 bits (1084), Expect = 2.8e-114
Identity = 201/219 (91.78%), Postives = 210/219 (95.89%), Query Frame = 0


Query: 1   MGKTESGVLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHG 60
           MGK ESGVLMLGLVV VIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHG
Sbjct: 1   MGKAESGVLMLGLVVVVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHG 60

Query: 61  LWPSNYSSARSQPCRGTRFD---IAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEK 120
            WPSNYS ARS+PCRG+RFD   IAPIRAQLNQYWPDVVNGN+QRFWEHEWSKHGTCSEK
Sbjct: 61  SWPSNYSRARSKPCRGSRFDITKIAPIRAQLNQYWPDVVNGNHQRFWEHEWSKHGTCSEK 120

Query: 121 QFPERAYFQNALDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFN 180
           QFP RAYFQNAL+IAKR+DLLSILK+AG NPDGKVKQRA+IEAAI++AIGKRPVLRCNFN
Sbjct: 121 QFPGRAYFQNALNIAKRHDLLSILKKAGHNPDGKVKQRASIEAAITKAIGKRPVLRCNFN 180

Query: 181 KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVWG 217
           KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVWG
Sbjct: 181 KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVWG 219

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BLAST of Moc08g22460.1 vs. NCBI nr
Match: XP_038906187.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 220.7 bits (561), Expect = 1.2e-53
Identity = 117/215 (54.42%), Postives = 138/215 (64.19%), Query Frame = 0


Query: 8   VLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHGLWPSNYS 67
           V+ +  +  ++    A YDYFQMV+QW PATCS   +R  CR +P  +FTIHGLWPSNYS
Sbjct: 7   VISIFSLALLVFSVKAQYDYFQMVQQWPPATCSGVGVR--CRAQPPSMFTIHGLWPSNYS 66

Query: 68  SARSQPCRGTRFD---IAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEKQFPERAY 127
           +A    C G  FD   IA IR QLN YWPDV+ GNNQRFW+HEW  HGTCS+  F +R Y
Sbjct: 67  TAH-LVCTGLPFDPTQIAAIRPQLNTYWPDVIRGNNQRFWQHEWDAHGTCSDPPFNQRQY 126

Query: 128 FQNALDIA--KRYDLLSILKRAGQNPDG-KVKQRANIEAAISRAIGKRPVLRCNFN-KSN 187
           FQ  L+I     YDLL+IL  AG  P    V+Q   IE AI  A GK P LRCN N ++ 
Sbjct: 127 FQTTLNIRMNHNYDLLAILNAAGLGPTATMVRQYQAIENAIQAATGKIPGLRCNTNAQTG 186

Query: 188 ARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVW 216
           +RQL EI+LC  KNGV L  CT FA  TCP  FVW
Sbjct: 187 SRQLFEIILCFDKNGVTLIDCTPFAGITCPLQFVW 218

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BLAST of Moc08g22460.1 vs. NCBI nr
Match: XP_022934593.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 213.8 bits (543), Expect = 1.5e-51
Identity = 113/225 (50.22%), Postives = 142/225 (63.11%), Query Frame = 0


Query: 1   MGKTESGVLMLGLV-VAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIH 60
           M K+ S V +   V V +I+C  A +DYFQMV+QW PA CS  K+R  C  +P  +FTIH
Sbjct: 1   MAKSPSIVSIFNFVFVVLILCVNAQFDYFQMVQQWPPAKCSSVKVR--CHVQPPTMFTIH 60

Query: 61  GLWPSNYSSAR----SQPCRGTRFDIAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCS 120
           GLWPSNYS+AR     QP   T+  IA +R QLN YWPDV+ G NQ+FW+HEW +HG CS
Sbjct: 61  GLWPSNYSNARLVCAPQPFNPTQ--IAALRPQLNTYWPDVIQGKNQQFWQHEWDRHGICS 120

Query: 121 EKQFPERAYFQNALDIAKR--YDLLSILKRAGQNP-DGKVKQRANIEAAISRAIGKRPVL 180
              F +  YFQ  L+I     YDLL+IL  AG  P D  +++  +IE AI  A GK+P L
Sbjct: 121 NPPFNQLQYFQITLNIRTNHSYDLLTILNAAGLGPSDTNIREYKDIEGAIQAATGKKPGL 180

Query: 181 RCNFN--KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVW 216
           RCN N      +QL EI+LC  K+G+ L  CT+FA  TCP  FVW
Sbjct: 181 RCNINAQSKEKKQLLEIILCFDKDGITLIDCTEFAGITCPPQFVW 221

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BLAST of Moc08g22460.1 vs. NCBI nr
Match: XP_023514499.1 (ribonuclease MC-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 211.1 bits (536), Expect = 9.7e-51
Identity = 112/219 (51.14%), Postives = 137/219 (62.56%), Query Frame = 0


Query: 1   MGKTESGVLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHG 60
           MGK    V +  L + + +C  A +DYFQMV+QW PATCS  +    C   P  +FTIHG
Sbjct: 1   MGKAPFIVSIFNLAL-LTLCVKAQFDYFQMVQQWPPATCSGVR----CYANPPAMFTIHG 60

Query: 61  LWPSNYSSARSQPCRGTRFDIAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEKQFP 120
           LWPSNYS+     C G  F+   +  QLN YWPDV++GNNQRFW+HEW  HGTCS+  F 
Sbjct: 61  LWPSNYSNV-PLICTGQPFN-PTLVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFN 120

Query: 121 ERAYFQNALDIA--KRYDLLSILKRAGQNPDG-KVKQRANIEAAISRAIGKRPVLRCNFN 180
           +  YFQ  L+I     YDLL+IL  AG  P G   +Q   IE AI  A GK+P LRCN N
Sbjct: 121 QLQYFQTTLNIRTNHNYDLLAILNTAGLGPTGTNTRQYGAIEGAIQAATGKKPGLRCNKN 180

Query: 181 -KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVW 216
            +S  +QL EI+LC  KNGV L  CT+FA  TCP+ FVW
Sbjct: 181 AQSKKKQLFEIILCFDKNGVTLIDCTQFAGITCPSQFVW 212

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BLAST of Moc08g22460.1 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 5.4e-36
Identity = 76/195 (38.97%), Postives = 112/195 (57.44%), Query Frame = 0


Query: 25  YDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHGLWPSNYSSARSQPCRGTRFDIAPI 84
           +D F  V+QW PA CS +K    C     + FTIHGLWP    ++ +   +G+ FDI  I
Sbjct: 1   FDSFWFVQQWPPAVCSFQK-SGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKI 60

Query: 85  ---RAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEKQFPERAYFQNALDIAKRYDLLSIL 144
              ++QLN  WP+V+  NNQ+FW HEW+KHGTCSE  F + AYF+ A+D+   YD++  L
Sbjct: 61  SHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGAL 120

Query: 145 KRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFN-KSNARQLHEIVLCVAKNGVALES 204
           +     P+G+ K R  I+  +    GK P LRC  + ++    L ++V C A++G  L  
Sbjct: 121 RPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLID 180

Query: 205 CTKFAAATCPNTFVW 216
           CT+    TC   F++
Sbjct: 181 CTR---DTCGANFIF 191

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BLAST of Moc08g22460.1 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 5.2e-31
Identity = 70/192 (36.46%), Postives = 104/192 (54.17%), Query Frame = 0


Query: 25  YDYFQMVEQWQPATC-SDKKLRFGCRQKPKQVFTIHGLWPSNYSSARSQPCRGTR-FD-- 84
           +D+F  V+QW  + C + KK  +    KP   F IHGLWP+         C  ++ FD  
Sbjct: 30  FDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASKPFDSS 89

Query: 85  -IAPIRAQLNQYWPDVV--NGNNQRFWEHEWSKHGTCSEKQFPERAYFQNALDIAKRYDL 144
            I+ +   + + WP +   +G+ + FWEHEW KHGTCSE    +  YFQ AL++ ++ +L
Sbjct: 90  TISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQKTNL 149

Query: 145 LSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFNKSNARQLHEIVLCVAKNGVA 204
           L  L +AG NPDGK     +I  +I  +IG  P + CN + S   QL+++ LCV ++G  
Sbjct: 150 LGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRSGSG 209

Query: 205 LESCTKFAAATC 210
           L  C  F    C
Sbjct: 210 LIECPVFPHGKC 221

[Close]

BLAST of Moc08g22460.1 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.9e-25
Identity = 65/209 (31.10%), Postives = 103/209 (49.28%), Query Frame = 0


Query: 8   VLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDK-KLRFGCRQKPKQVFTIHGLWPSNY 67
           + +L L    +   A  +D+F  V QW  A C  +    +    KP   F IHGLWP+  
Sbjct: 5   IFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYK 64

Query: 68  SSARSQPCR-GTRFD---IAPIRAQLNQYWPDV--VNGNNQRFWEHEWSKHGTCSEKQFP 127
           +    Q C   +RFD   ++ + + L + WP +   + +  +FW HEW KHGTC+E +  
Sbjct: 65  TGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELD 124

Query: 128 ERAYFQNALDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFNKSN 187
           +  YF+  L + ++ +LL  L  AG  PD K  +  +IE  I + +G  P + CN + S+
Sbjct: 125 QHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSH 184

Query: 188 ARQLHEIVLCVAKNGVALESCTKFAAATC 210
             QL++I LCV  +     +C       C
Sbjct: 185 NSQLYQIYLCVDTSASKFINCPVMPHGRC 213

[Close]

BLAST of Moc08g22460.1 vs. ExPASy Swiss-Prot
Match: O80322 (Ribonuclease S-1 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 7.3e-25
Identity = 62/197 (31.47%), Postives = 99/197 (50.25%), Query Frame = 0


Query: 2   GKTESGVLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHGL 61
           G T    ++  L+V ++      YDYFQ  +Q+QPA C+       C     ++FT+HGL
Sbjct: 5   GMTYMFTMVFSLIVLILSSSTVGYDYFQFTQQYQPAVCNSNPT--PCNDPTDKLFTVHGL 64

Query: 62  WPSNYSSARSQPCRGTRFD---IAPIRAQLNQYWPDVVN-GNNQRFWEHEWSKHGTCSEK 121
           WPSN +    + C+ T  +   I  + AQL   WP+V+N  ++  FWE EW KHGTC   
Sbjct: 65  WPSNRNGPDPEKCKTTALNSQKIGNMTAQLEIIWPNVLNRSDHVGFWEKEWIKHGTCGYP 124

Query: 122 QFPERAYFQNA---LDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAISRAIGK-RPVLR 181
              +  ++      + I ++ ++ +IL +A   P+G  +   +IE AI R     +P  +
Sbjct: 125 TIKDDMHYLQTVIRMYITQKQNVSAILSKAAIQPNGTNRPLVDIENAIRRGTNNTKPKFK 184

Query: 182 CNFNKSNARQLHEIVLC 191
           C  N     +L E+ LC
Sbjct: 185 CQKNTRTTTELVEVTLC 199

[Close]

BLAST of Moc08g22460.1 vs. ExPASy Swiss-Prot
Match: Q40966 (Ribonuclease S-4 OS=Pyrus pyrifolia OX=3767 PE=1 SV=2)

HSP 1 Score: 113.2 bits (282), Expect = 3.6e-24
Identity = 63/208 (30.29%), Postives = 103/208 (49.52%), Query Frame = 0


Query: 2   GKTESGVLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHGL 61
           G T    ++L L+V +       +DYFQ  +Q+QPA C+       C     ++FT+HGL
Sbjct: 5   GMTYMFTMVLSLIVLIFSASTVGFDYFQFTQQYQPAVCNSNPT--PCNDPTDKLFTVHGL 64

Query: 62  WPSNYSSARSQPCRGTRFD---IAPIRAQLNQYWPDVVN-GNNQRFWEHEWSKHGTCSEK 121
           WPSN +    + C+ T  +   I  + AQL   WP+V+N  ++  FWE EW KHGTC   
Sbjct: 65  WPSNRNGPDPEKCKTTTMNSQKIGNMTAQLEIIWPNVLNRSDHVGFWEREWLKHGTCGYP 124

Query: 122 QFPERAYFQNA---LDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAI-SRAIGKRPVLR 181
              +  ++      + I ++ ++ +IL +A   P+G  +   +IE AI S     +P  +
Sbjct: 125 TIKDDMHYLKTVIKMYITQKQNVSAILSKATIQPNGNNRSLVDIENAIRSGNNNTKPKFK 184

Query: 182 CNFNKSNARQLHEIVLCVAKNGVALESC 202
           C  N     +L E+ LC  ++     +C
Sbjct: 185 CQKNTRTTTELVEVTLCSNRDLTKFINC 210

[Close]

BLAST of Moc08g22460.1 vs. ExPASy TrEMBL
Match: A0A6J1DZ67 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111025626 PE=3 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 4.6e-123
Identity = 217/220 (98.64%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 1   MGKTESGVLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHG 60
           MGKTESGVLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHG
Sbjct: 1   MGKTESGVLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHG 60

Query: 61  LWPSNYSSARSQPCRGTRFD---IAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEK 120
           LWPSNYSSARSQPCRGTRFD   IAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEK
Sbjct: 61  LWPSNYSSARSQPCRGTRFDVTQIAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEK 120

Query: 121 QFPERAYFQNALDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFN 180
           QFPERAYFQNALDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFN
Sbjct: 121 QFPERAYFQNALDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFN 180

Query: 181 KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVWGS 218
           KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVWGS
Sbjct: 181 KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVWGS 220

[Close]

BLAST of Moc08g22460.1 vs. ExPASy TrEMBL
Match: A0A6J1DXG5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025393 PE=3 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.3e-114
Identity = 201/219 (91.78%), Postives = 210/219 (95.89%), Query Frame = 0


Query: 1   MGKTESGVLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHG 60
           MGK ESGVLMLGLVV VIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHG
Sbjct: 1   MGKAESGVLMLGLVVVVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHG 60

Query: 61  LWPSNYSSARSQPCRGTRFD---IAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEK 120
            WPSNYS ARS+PCRG+RFD   IAPIRAQLNQYWPDVVNGN+QRFWEHEWSKHGTCSEK
Sbjct: 61  SWPSNYSRARSKPCRGSRFDITKIAPIRAQLNQYWPDVVNGNHQRFWEHEWSKHGTCSEK 120

Query: 121 QFPERAYFQNALDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFN 180
           QFP RAYFQNAL+IAKR+DLLSILK+AG NPDGKVKQRA+IEAAI++AIGKRPVLRCNFN
Sbjct: 121 QFPGRAYFQNALNIAKRHDLLSILKKAGHNPDGKVKQRASIEAAITKAIGKRPVLRCNFN 180

Query: 181 KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVWG 217
           KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVWG
Sbjct: 181 KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVWG 219

[Close]

BLAST of Moc08g22460.1 vs. ExPASy TrEMBL
Match: A0A6J1F837 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 7.3e-52
Identity = 113/225 (50.22%), Postives = 142/225 (63.11%), Query Frame = 0


Query: 1   MGKTESGVLMLGLV-VAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIH 60
           M K+ S V +   V V +I+C  A +DYFQMV+QW PA CS  K+R  C  +P  +FTIH
Sbjct: 1   MAKSPSIVSIFNFVFVVLILCVNAQFDYFQMVQQWPPAKCSSVKVR--CHVQPPTMFTIH 60

Query: 61  GLWPSNYSSAR----SQPCRGTRFDIAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCS 120
           GLWPSNYS+AR     QP   T+  IA +R QLN YWPDV+ G NQ+FW+HEW +HG CS
Sbjct: 61  GLWPSNYSNARLVCAPQPFNPTQ--IAALRPQLNTYWPDVIQGKNQQFWQHEWDRHGICS 120

Query: 121 EKQFPERAYFQNALDIAKR--YDLLSILKRAGQNP-DGKVKQRANIEAAISRAIGKRPVL 180
              F +  YFQ  L+I     YDLL+IL  AG  P D  +++  +IE AI  A GK+P L
Sbjct: 121 NPPFNQLQYFQITLNIRTNHSYDLLTILNAAGLGPSDTNIREYKDIEGAIQAATGKKPGL 180

Query: 181 RCNFN--KSNARQLHEIVLCVAKNGVALESCTKFAAATCPNTFVW 216
           RCN N      +QL EI+LC  K+G+ L  CT+FA  TCP  FVW
Sbjct: 181 RCNINAQSKEKKQLLEIILCFDKDGITLIDCTEFAGITCPPQFVW 221

[Close]

BLAST of Moc08g22460.1 vs. ExPASy TrEMBL
Match: A0A6J1IAR7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 6.2e-51
Identity = 110/209 (52.63%), Postives = 132/209 (63.16%), Query Frame = 0


Query: 14  VVAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHGLWPSNYSSARSQP 73
           +V + I   A +DYFQMV+QW PATCS  +    C   P  +FTIHGLWPS YS+     
Sbjct: 14  LVLLTIGVKAQFDYFQMVQQWPPATCSGVR----CYANPPAMFTIHGLWPSTYSNV-PPV 73

Query: 74  CRGTRFD---IAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEKQFPERAYFQNALD 133
           C G  FD   IA +  QLN YWPDV+NGNNQRFW+HEW  HGTCS+  F +  YFQ  L+
Sbjct: 74  CAGQPFDRAQIATLEPQLNTYWPDVINGNNQRFWKHEWDNHGTCSDPPFNQLQYFQTTLN 133

Query: 134 IA--KRYDLLSILKRAGQNPDG-KVKQRANIEAAISRAIGKRPVLRCNFN-KSNARQLHE 193
           I     YDLL+IL  AG  P     +Q   IE AI  A GK+P LRCN N +S  +QL E
Sbjct: 134 IRTNHNYDLLAILNTAGLGPTATTTRQYQAIEGAIQAATGKKPGLRCNKNAQSKRKQLFE 193

Query: 194 IVLCVAKNGVALESCTKFAAATCPNTFVW 216
           I+LC  KNGV L +CT+FA  TCP+ FVW
Sbjct: 194 IILCFDKNGVTLINCTQFAGITCPSQFVW 217

[Close]

BLAST of Moc08g22460.1 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 8.0e-51
Identity = 109/208 (52.40%), Postives = 131/208 (62.98%), Query Frame = 0


Query: 15  VAVIICEAAAYDYFQMVEQWQPATCSDKKLRFGCRQKPKQVFTIHGLWPSNYSSARSQPC 74
           V +  C  A +DYFQMV+QW PATCS  +    C   P  +FTIHGLWPSNYS+     C
Sbjct: 14  VLLTSCVKAQFDYFQMVQQWPPATCSGVR----CYANPPAMFTIHGLWPSNYSNV-PLVC 73

Query: 75  RGTRFD---IAPIRAQLNQYWPDVVNGNNQRFWEHEWSKHGTCSEKQFPERAYFQNALDI 134
            G  F+   IA +  QLN YWPDV++GNNQRFW+HEW  HGTCS+  F +  YFQ  L+I
Sbjct: 74  AGQPFNPAQIATLVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQLQYFQTTLNI 133

Query: 135 A--KRYDLLSILKRAGQNPDG-KVKQRANIEAAISRAIGKRPVLRCNFN-KSNARQLHEI 194
                YDLL+IL  AG  P     +Q   IE AI  A GK+P LRCN N +S  +QL EI
Sbjct: 134 RTNHNYDLLAILNTAGLGPTATNTRQYGAIEGAIQAATGKKPGLRCNKNAQSKKKQLFEI 193

Query: 195 VLCVAKNGVALESCTKFAAATCPNTFVW 216
           +LC  KNGV L  CT+FA  TCP+ FVW
Sbjct: 194 ILCFDKNGVTLIDCTQFAGITCPSQFVW 216

[Close]

BLAST of Moc08g22460.1 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 136.0 bits (341), Expect = 3.7e-32
Identity = 70/192 (36.46%), Postives = 104/192 (54.17%), Query Frame = 0


Query: 25  YDYFQMVEQWQPATC-SDKKLRFGCRQKPKQVFTIHGLWPSNYSSARSQPCRGTR-FD-- 84
           +D+F  V+QW  + C + KK  +    KP   F IHGLWP+         C  ++ FD  
Sbjct: 30  FDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASKPFDSS 89

Query: 85  -IAPIRAQLNQYWPDVV--NGNNQRFWEHEWSKHGTCSEKQFPERAYFQNALDIAKRYDL 144
            I+ +   + + WP +   +G+ + FWEHEW KHGTCSE    +  YFQ AL++ ++ +L
Sbjct: 90  TISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQKTNL 149

Query: 145 LSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFNKSNARQLHEIVLCVAKNGVA 204
           L  L +AG NPDGK     +I  +I  +IG  P + CN + S   QL+++ LCV ++G  
Sbjct: 150 LGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRSGSG 209

Query: 205 LESCTKFAAATC 210
           L  C  F    C
Sbjct: 210 LIECPVFPHGKC 221

[Close]

BLAST of Moc08g22460.1 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.3e-29
Identity = 68/194 (35.05%), Postives = 97/194 (50.00%), Query Frame = 0


Query: 25  YDYFQMVEQWQPATC-SDKKLRFGCRQKPKQVFTIHGLWPSNYSSARSQPC----RGTRF 84
           +D+F  V QW  A C + +   +    KP   F IHGLWP+    +    C    +  R 
Sbjct: 28  FDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPNYNGGSWPSNCDPDSQFDRS 87

Query: 85  DIAPIRAQLNQYWPDVVNGNNQ--RFWEHEWSKHGTCSEKQFPERAYFQNALDIAKRYDL 144
            I+ + + L + WP +   +N+   FWEHEW KHGTCSE    +  YF+NAL + ++ +L
Sbjct: 88  QISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHEYFENALKLKQKANL 147

Query: 145 LSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFNKSNARQLHEIVLCVAKNGVA 204
           L ILK +G NPD        I  AI   IG  P + CN +     QLH+I +CV  +G  
Sbjct: 148 LQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDPERNAQLHQIYICVDTSGTE 207

Query: 205 LESCTKFAAATCPN 212
              C      +CP+
Sbjct: 208 FIECPVLPRGSCPS 221

[Close]

BLAST of Moc08g22460.1 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 117.5 bits (293), Expect = 1.4e-26
Identity = 65/209 (31.10%), Postives = 103/209 (49.28%), Query Frame = 0


Query: 8   VLMLGLVVAVIICEAAAYDYFQMVEQWQPATCSDK-KLRFGCRQKPKQVFTIHGLWPSNY 67
           + +L L    +   A  +D+F  V QW  A C  +    +    KP   F IHGLWP+  
Sbjct: 5   IFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYK 64

Query: 68  SSARSQPCR-GTRFD---IAPIRAQLNQYWPDV--VNGNNQRFWEHEWSKHGTCSEKQFP 127
           +    Q C   +RFD   ++ + + L + WP +   + +  +FW HEW KHGTC+E +  
Sbjct: 65  TGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELD 124

Query: 128 ERAYFQNALDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAISRAIGKRPVLRCNFNKSN 187
           +  YF+  L + ++ +LL  L  AG  PD K  +  +IE  I + +G  P + CN + S+
Sbjct: 125 QHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSH 184

Query: 188 ARQLHEIVLCVAKNGVALESCTKFAAATC 210
             QL++I LCV  +     +C       C
Sbjct: 185 NSQLYQIYLCVDTSASKFINCPVMPHGRC 213

[Close]

BLAST of Moc08g22460.1 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 94.0 bits (232), Expect = 1.6e-19
Identity = 62/220 (28.18%), Postives = 98/220 (44.55%), Query Frame = 0


Query: 7   GVLMLGLVVAVIICEAAA-----YDYFQMVEQWQPATCSDKKLRFGCRQKPK----QVFT 66
           G++++ L++   +  +++     +++F  V  W  A C  +K   GC    K      F 
Sbjct: 3   GIIIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQK---GCCPPTKGNTASDFI 62

Query: 67  IHGLWPSNYSSARSQPCRGTR-FDIAPIR---AQLNQYWPD----VVNGNNQRFWEHEWS 126
           IHGLWP   +      C  T  FDI+ I     Q+ + W +        N    WEHEW+
Sbjct: 63  IHGLWPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWN 122

Query: 127 KHGTCSEKQFPERAYFQNALDIAKRYDLLSILKRAGQNPDGKVKQRANIEAAISRAIGKR 186
           KHGTC +  F + +YF+  L    +  LL+IL + G  P+        I+ AI  AIG  
Sbjct: 123 KHGTCVQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFA 182

Query: 187 PVLRCNFNKSNARQLHEIVLCVAKNGVALESCTKFAAATC 210
           P + CN +    +QL +I +C+         C      +C
Sbjct: 183 PGIECNEDVKGNKQLFQIYICLDNYAKEFVECPYVPDKSC 219

[Close]

BLAST of Moc08g22460.1 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 77.4 bits (189), Expect = 1.6e-14
Identity = 60/189 (31.75%), Postives = 82/189 (43.39%), Query Frame = 0


Query: 25  YDYFQMVEQWQPAT-------CSDKKLRFGCRQKPKQVFTIHGLWPSNYSSARSQPCRGT 84
           +DYF +  QW P T       C  K         P Q FTIHGLWP     +    C  +
Sbjct: 32  FDYFALSLQW-PGTYCRGTRHCCSKNACCRGSDAPTQ-FTIHGLWPDYNDGSWPSCCYRS 91

Query: 85  RF---DIAPIRAQLNQYWPDV-------VNGNNQRFWEHEWSKHGTCSEKQF-PERAYFQ 144
            F   +I+ +   L +YWP +        NG    FW HEW KHGTCS   F  E  YF 
Sbjct: 92  DFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFL 151

Query: 145 NALDIAKRYDLLSILKRAGQNPDGKVK-QRANIEAAISRAIGKRPVLRCNFNKSNARQLH 195
             L++  ++++  +L +AG       K     I  AI  A    P + C   K +A  + 
Sbjct: 152 TTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVC---KRDA--ID 211

The following BLAST results are available for this feature:

BLAST of Moc08g22460.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159207.1	9.5e-123	98.64	ribonuclease MC-like [Momordica charantia]	[more]
XP_022158938.1	2.8e-114	91.78	ribonuclease MC-like [Momordica charantia]	[more]
XP_038906187.1	1.2e-53	54.42	ribonuclease MC-like [Benincasa hispida]	[more]
XP_022934593.1	1.5e-51	50.22	ribonuclease MC-like [Cucurbita moschata]	[more]
XP_023514499.1	9.7e-51	51.14	ribonuclease MC-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc08g22460.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	5.4e-36	38.97	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	5.2e-31	36.46	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P42815	1.9e-25	31.10	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
O80322	7.3e-25	31.47	Ribonuclease S-1 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]
Q40966	3.6e-24	30.29	Ribonuclease S-4 OS=Pyrus pyrifolia OX=3767 PE=1 SV=2	[more]

BLAST of Moc08g22460.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DZ67	4.6e-123	98.64	Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111025626 PE=3 SV=1	[more]
A0A6J1DXG5	1.3e-114	91.78	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025393 PE=3 SV=1	[more]
A0A6J1F837	7.3e-52	50.22	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1	[more]
A0A6J1IAR7	6.2e-51	52.63	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1	[more]
A0A6J1EXD5	8.0e-51	52.40	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1	[more]

BLAST of Moc08g22460.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	3.7e-32	36.46	ribonuclease 1	[more]
AT1G14220.1	1.3e-29	35.05	Ribonuclease T2 family protein	[more]
AT1G26820.1	1.4e-26	31.10	ribonuclease 3	[more]
AT1G14210.1	1.6e-19	28.18	Ribonuclease T2 family protein	[more]
AT2G39780.1	1.6e-14	31.75	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 25..201 e-value: 4.5E-42 score: 144.3
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 17..210
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 25..209 e-value: 1.0E-54 score: 187.4
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 19..215
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 17..210
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 56..63
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 103..114
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 25..215 e-value: 4.75222E-68 score: 205.259

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc08g22460	Moc08g22460	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc08g22460.1.exon2	Moc08g22460.1.exon2	exon
Moc08g22460.1.exon1	Moc08g22460.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc08g22460.1	cds.Moc08g22460.1_2	CDS
cds.Moc08g22460.1	cds.Moc08g22460.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc08g22460.1	Moc08g22460.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding