Moc08g13720.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g13720.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Cytochrome p450
Location	chr8: 10417076 .. 10417408 (+)
Sequence length	333
RNA-Seq Expression	Moc08g13720.1
Synteny	Moc08g13720.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAGGATGGAGACATTGTGGGGGAAGGACCGGTTCGAGTTTAAACCGGGCCGGTGGCTGGCCGAACCGGATGGCCGGGTGAAGTTGGTGAGTCCATACAAATTTCCGATATTTCAGGCCGGTCCGAGAGTTTGTCTGGGGAAGGAAATGGCTTTTATTCAGATGAAGTATGTGGTGGCTTCAATTCTGGGCCGGTTCAGAATCAGACCCGTCGCTGTGGACGACCCGGTTTTCGTGCCGCTGTTGACGGCCCACATGGCCGGCGGCTTCAGAGTTTTGTTTCAGAGGAGAGAGAGGGCCGGTTTAAGAGAGAAAAATTCCCCAATTTGA

mRNA sequence

ATGGGGAGGATGGAGACATTGTGGGGGAAGGACCGGTTCGAGTTTAAACCGGGCCGGTGGCTGGCCGAACCGGATGGCCGGGTGAAGTTGGTGAGTCCATACAAATTTCCGATATTTCAGGCCGGTCCGAGAGTTTGTCTGGGGAAGGAAATGGCTTTTATTCAGATGAAGTATGTGGTGGCTTCAATTCTGGGCCGGTTCAGAATCAGACCCGTCGCTGTGGACGACCCGGTTTTCGTGCCGCTGTTGACGGCCCACATGGCCGGCGGCTTCAGAGTTTTGTTTCAGAGGAGAGAGAGGGCCGGTTTAAGAGAGAAAAATTCCCCAATTTGA

Coding sequence (CDS)

ATGGGGAGGATGGAGACATTGTGGGGGAAGGACCGGTTCGAGTTTAAACCGGGCCGGTGGCTGGCCGAACCGGATGGCCGGGTGAAGTTGGTGAGTCCATACAAATTTCCGATATTTCAGGCCGGTCCGAGAGTTTGTCTGGGGAAGGAAATGGCTTTTATTCAGATGAAGTATGTGGTGGCTTCAATTCTGGGCCGGTTCAGAATCAGACCCGTCGCTGTGGACGACCCGGTTTTCGTGCCGCTGTTGACGGCCCACATGGCCGGCGGCTTCAGAGTTTTGTTTCAGAGGAGAGAGAGGGCCGGTTTAAGAGAGAAAAATTCCCCAATTTGA

Protein sequence

MGRMETLWGKDRFEFKPGRWLAEPDGRVKLVSPYKFPIFQAGPRVCLGKEMAFIQMKYVVASILGRFRIRPVAVDDPVFVPLLTAHMAGGFRVLFQRRERAGLREKNSPI

Homology

[Close]

BLAST of Moc08g13720.1 vs. NCBI nr
Match: XP_022982702.1 (cytochrome P450 94B3 [Cucurbita maxima])

HSP 1 Score: 184.1 bits (466), Expect = 6.5e-43
Identity = 89/115 (77.39%), Postives = 95/115 (82.61%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR-----VKLVSPYKFPIFQAGPRVCLGKEMAFIQ 60
           MGRME LWGKD FEFKP RWL EPDGR     VKLVSPYKFPIFQAGPRVCLGKEMAFIQ
Sbjct: 392 MGRMECLWGKDWFEFKPSRWLLEPDGRGWRRGVKLVSPYKFPIFQAGPRVCLGKEMAFIQ 451

Query: 61  MKYVVASILGRFRIRPVAVDDPVFVPLLTAHMAGGFRVLFQRRERAGLREKNSPI 111
           MKYVVASIL +FRI+P+A D PVFVPLLTAHMAGGF+VL QRRE+     +  PI
Sbjct: 452 MKYVVASILSQFRIKPIAADHPVFVPLLTAHMAGGFKVLCQRREKEERERERKPI 506

[Close]

BLAST of Moc08g13720.1 vs. NCBI nr
Match: KAG6588901.1 (Cytochrome P450 94B3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 183.3 bits (464), Expect = 1.1e-42
Identity = 89/108 (82.41%), Postives = 95/108 (87.96%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR-----VKLVSPYKFPIFQAGPRVCLGKEMAFIQ 60
           MGRME +WGKDRFEFKP RWL EP+GR     VKLVSPYKFPIFQAGPRVCLGKEMAFIQ
Sbjct: 391 MGRMEAVWGKDRFEFKPSRWLLEPEGRGRRRGVKLVSPYKFPIFQAGPRVCLGKEMAFIQ 450

Query: 61  MKYVVASILGRFRIRPVAV---DDPVFVPLLTAHMAGGFRVLFQRRER 101
           MKYVVASIL RFRI+P+A    DDPVFVPLLTAHMAGGF+VLFQRRE+
Sbjct: 451 MKYVVASILSRFRIKPIAAAASDDPVFVPLLTAHMAGGFKVLFQRREK 498

[Close]

BLAST of Moc08g13720.1 vs. NCBI nr
Match: XP_022928304.1 (cytochrome P450 94B3-like [Cucurbita moschata])

HSP 1 Score: 182.2 bits (461), Expect = 2.5e-42
Identity = 90/119 (75.63%), Postives = 98/119 (82.35%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR-----VKLVSPYKFPIFQAGPRVCLGKEMAFIQ 60
           MGRME +WGKDRFEFKP RWL EP+GR     VKLVSPYKFPIFQAGPRVCLGKEMAFIQ
Sbjct: 399 MGRMEEVWGKDRFEFKPSRWLLEPEGRGRRRGVKLVSPYKFPIFQAGPRVCLGKEMAFIQ 458

Query: 61  MKYVVASILGRFRIRPVAV------DDPVFVPLLTAHMAGGFRVLFQRRERAGLREKNS 109
           MKYVVASIL RFRI+P+A       DDPVFVPLLTAHMAGGF+VLFQRRE+     K++
Sbjct: 459 MKYVVASILSRFRIKPIATAAAAASDDPVFVPLLTAHMAGGFKVLFQRREKERENSKHT 517

[Close]

BLAST of Moc08g13720.1 vs. NCBI nr
Match: XP_023529331.1 (cytochrome P450 94B3-like [Cucurbita pepo subsp. pepo] >XP_023529333.1 cytochrome P450 94B3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 181.8 bits (460), Expect = 3.2e-42
Identity = 89/116 (76.72%), Postives = 97/116 (83.62%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR-----VKLVSPYKFPIFQAGPRVCLGKEMAFIQ 60
           MGRME +WGKDRFEFKP RWL EP+GR     VKLVSPYKFPIFQAGPRVCLGKEMAFIQ
Sbjct: 398 MGRMEAVWGKDRFEFKPSRWLLEPEGRGRRRGVKLVSPYKFPIFQAGPRVCLGKEMAFIQ 457

Query: 61  MKYVVASILGRFRIRPVAV----DDPVFVPLLTAHMAGGFRVLFQRRERAGLREKN 108
           MKYVVASIL +FRI+P+      DDPVFVPLLTAHMAGGF+VLFQRRE+   RE +
Sbjct: 458 MKYVVASILSQFRIKPIVAASDSDDPVFVPLLTAHMAGGFKVLFQRREKEKERENS 513

[Close]

BLAST of Moc08g13720.1 vs. NCBI nr
Match: KAG6581095.1 (Cytochrome P450 94B1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 180.6 bits (457), Expect = 7.1e-42
Identity = 87/105 (82.86%), Postives = 92/105 (87.62%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR-----VKLVSPYKFPIFQAGPRVCLGKEMAFIQ 60
           MGRME LWGKD FEFKP RWL EPDGR     VKLVSPYKFPIFQAGPRVCLGKEMAFIQ
Sbjct: 392 MGRMERLWGKDWFEFKPSRWLLEPDGRGRRRGVKLVSPYKFPIFQAGPRVCLGKEMAFIQ 451

Query: 61  MKYVVASILGRFRIRPVAVDDPVFVPLLTAHMAGGFRVLFQRRER 101
           MKYVVASIL +FRI+P+A D PVFVPLLTAHMAGGF+VL QRRE+
Sbjct: 452 MKYVVASILSQFRIKPIAADHPVFVPLLTAHMAGGFKVLCQRREK 496

[Close]

BLAST of Moc08g13720.1 vs. ExPASy Swiss-Prot
Match: Q9SMP5 (Cytochrome P450 94B3 OS=Arabidopsis thaliana OX=3702 GN=CYP94B3 PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 3.7e-33
Identity = 68/102 (66.67%), Postives = 76/102 (74.51%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR----VKLVSPYKFPIFQAGPRVCLGKEMAFIQM 60
           MGRMETLWG D  EF P RW     G     +K +SPYKFP+FQAGPRVC+GKEMAF+QM
Sbjct: 398 MGRMETLWGTDSEEFNPNRWFDSEPGSTRPVLKPISPYKFPVFQAGPRVCVGKEMAFMQM 457

Query: 61  KYVVASILGRFRIRPVAVDDPVFVPLLTAHMAGGFRVLFQRR 99
           KYVV S+L RF I PV  D PVFVPLLTAHMAGG +V  +RR
Sbjct: 458 KYVVGSVLSRFEIVPVNKDRPVFVPLLTAHMAGGLKVKIKRR 499

[Close]

BLAST of Moc08g13720.1 vs. ExPASy Swiss-Prot
Match: Q9FMV7 (Cytochrome P450 94B1 OS=Arabidopsis thaliana OX=3702 GN=CYP94B1 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 4.1e-32
Identity = 67/105 (63.81%), Postives = 78/105 (74.29%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR-----VKLVSPYKFPIFQAGPRVCLGKEMAFIQ 60
           MGRME +WGKD  EFKP RW  E         +K VS +KFP+FQAGPRVC+GKEMAF Q
Sbjct: 400 MGRMEKVWGKDWDEFKPNRWFEEEPSYGTKPVLKSVSSFKFPVFQAGPRVCIGKEMAFTQ 459

Query: 61  MKYVVASILGRFRIRPVAVDDPVFVPLLTAHMAGGFRVLFQRRER 101
           MKYVV S+L RF+I PV  + PVFVPLLTAHMAGG +V  +RRE+
Sbjct: 460 MKYVVGSVLSRFKIIPVCNNRPVFVPLLTAHMAGGLKVKIKRREQ 504

[Close]

BLAST of Moc08g13720.1 vs. ExPASy Swiss-Prot
Match: O81117 (Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 9.1e-24
Identity = 53/105 (50.48%), Postives = 72/105 (68.57%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGRVKLV----SPYKFPIFQAGPRVCLGKEMAFIQM 60
           MGRM++LWG D  EF+P RWL + +   K V      Y +P+FQAGPRVCLGKEMAF+QM
Sbjct: 409 MGRMKSLWGDDWAEFRPERWLEKDEVNGKWVFVGRDSYSYPVFQAGPRVCLGKEMAFMQM 468

Query: 61  KYVVASILGRFRIRPVA--VDDPVFVPLLTAHMAGGFRVLFQRRE 100
           K +VA I+G+F++ P A    +P F+  L++ M GGF V  Q+R+
Sbjct: 469 KRIVAGIVGKFKVVPEAHLAQEPGFISFLSSQMEGGFPVTIQKRD 513

[Close]

BLAST of Moc08g13720.1 vs. ExPASy Swiss-Prot
Match: P98188 (Cytochrome P450 94A2 OS=Vicia sativa OX=3908 GN=CYP94A2 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 8.5e-22
Identity = 52/103 (50.49%), Postives = 69/103 (66.99%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPD-GRVKLV--SPYKFPIFQAGPRVCLGKEMAFIQMK 60
           MGR E +WG D  EF+P RWL+  + G+   V    Y +P+FQAGPRVC+GKEMAF+QMK
Sbjct: 407 MGRSEKIWGPDWAEFRPERWLSRDEVGKWSFVGIDYYSYPVFQAGPRVCIGKEMAFLQMK 466

Query: 61  YVVASILGRFRIRPVAVD--DPVFVPLLTAHMAGGFRVLFQRR 99
            VVA I+GRFR+ P  V+  +P +    T+ M GGF V  ++R
Sbjct: 467 RVVAGIMGRFRVVPAMVEGIEPEYTAHFTSVMKGGFPVKIEKR 509

[Close]

BLAST of Moc08g13720.1 vs. ExPASy Swiss-Prot
Match: Q9ZUX1 (Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 2.3e-19
Identity = 47/101 (46.53%), Postives = 65/101 (64.36%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGRVKLVSPYKFPIFQAGPRVCLGKEMAFIQMKYVV 60
           MGRM+ +WG D  EFKP RWL + +G+ +  +P K+P+FQAG RVC+GKEMA ++MK + 
Sbjct: 395 MGRMDRIWGPDYEEFKPERWL-DNEGKFRPENPVKYPVFQAGARVCIGKEMAIMEMKSIA 454

Query: 61  ASILGRFRIR---PVAVDDPVFVPLLTAHMAGGFRVLFQRR 99
            +I+ RF  R   P   +   F P LTA + GG  V+ Q R
Sbjct: 455 VAIIRRFETRVASPETTETLRFAPGLTATVNGGLPVMIQER 494

[Close]

BLAST of Moc08g13720.1 vs. ExPASy TrEMBL
Match: A0A6J1IXA5 (cytochrome P450 94B3 OS=Cucurbita maxima OX=3661 GN=LOC111481487 PE=3 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 3.1e-43
Identity = 89/115 (77.39%), Postives = 95/115 (82.61%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR-----VKLVSPYKFPIFQAGPRVCLGKEMAFIQ 60
           MGRME LWGKD FEFKP RWL EPDGR     VKLVSPYKFPIFQAGPRVCLGKEMAFIQ
Sbjct: 392 MGRMECLWGKDWFEFKPSRWLLEPDGRGWRRGVKLVSPYKFPIFQAGPRVCLGKEMAFIQ 451

Query: 61  MKYVVASILGRFRIRPVAVDDPVFVPLLTAHMAGGFRVLFQRRERAGLREKNSPI 111
           MKYVVASIL +FRI+P+A D PVFVPLLTAHMAGGF+VL QRRE+     +  PI
Sbjct: 452 MKYVVASILSQFRIKPIAADHPVFVPLLTAHMAGGFKVLCQRREKEERERERKPI 506

[Close]

BLAST of Moc08g13720.1 vs. ExPASy TrEMBL
Match: A0A6J1EKG0 (cytochrome P450 94B3-like OS=Cucurbita moschata OX=3662 GN=LOC111435168 PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.2e-42
Identity = 90/119 (75.63%), Postives = 98/119 (82.35%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR-----VKLVSPYKFPIFQAGPRVCLGKEMAFIQ 60
           MGRME +WGKDRFEFKP RWL EP+GR     VKLVSPYKFPIFQAGPRVCLGKEMAFIQ
Sbjct: 399 MGRMEEVWGKDRFEFKPSRWLLEPEGRGRRRGVKLVSPYKFPIFQAGPRVCLGKEMAFIQ 458

Query: 61  MKYVVASILGRFRIRPVAV------DDPVFVPLLTAHMAGGFRVLFQRRERAGLREKNS 109
           MKYVVASIL RFRI+P+A       DDPVFVPLLTAHMAGGF+VLFQRRE+     K++
Sbjct: 459 MKYVVASILSRFRIKPIATAAAAASDDPVFVPLLTAHMAGGFKVLFQRREKERENSKHT 517

[Close]

BLAST of Moc08g13720.1 vs. ExPASy TrEMBL
Match: A0A6J1F3A4 (cytochrome P450 94B1-like OS=Cucurbita moschata OX=3662 GN=LOC111441980 PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 3.5e-42
Identity = 87/105 (82.86%), Postives = 92/105 (87.62%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR-----VKLVSPYKFPIFQAGPRVCLGKEMAFIQ 60
           MGRME LWGKD FEFKP RWL EPDGR     VKLVSPYKFPIFQAGPRVCLGKEMAFIQ
Sbjct: 392 MGRMERLWGKDWFEFKPSRWLLEPDGRGRRRGVKLVSPYKFPIFQAGPRVCLGKEMAFIQ 451

Query: 61  MKYVVASILGRFRIRPVAVDDPVFVPLLTAHMAGGFRVLFQRRER 101
           MKYVVASIL +FRI+P+A D PVFVPLLTAHMAGGF+VL QRRE+
Sbjct: 452 MKYVVASILSQFRIKPIAADHPVFVPLLTAHMAGGFKVLCQRREK 496

[Close]

BLAST of Moc08g13720.1 vs. ExPASy TrEMBL
Match: A0A6J1JG29 (cytochrome P450 94B1-like OS=Cucurbita maxima OX=3661 GN=LOC111486590 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 5.0e-41
Identity = 86/112 (76.79%), Postives = 96/112 (85.71%), Query Frame = 0


Query: 3   RMETLWGKDRFEFKPGRWLAEPDGR-----VKLVSPYKFPIFQAGPRVCLGKEMAFIQMK 62
           RME +WGKDRFEFKP RWL EP+GR     VKLVSPYKFPIFQAGPRVCLGKEMAFIQMK
Sbjct: 26  RMEAVWGKDRFEFKPSRWLLEPEGRGRRRGVKLVSPYKFPIFQAGPRVCLGKEMAFIQMK 85

Query: 63  YVVASILGRFRIRPVAV---DDPVFVPLLTAHMAGGFRVLFQRRERAGLREK 107
           YVVASIL +FRI+P+A    +DPVFVPLLTAHMAGGF+VLFQRRE+   +E+
Sbjct: 86  YVVASILSQFRIKPIAATASNDPVFVPLLTAHMAGGFKVLFQRREKEKEKER 137

[Close]

BLAST of Moc08g13720.1 vs. ExPASy TrEMBL
Match: A0A0A0LBW1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G585890 PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 8.5e-41
Identity = 86/111 (77.48%), Postives = 92/111 (82.88%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR------VKLVSPYKFPIFQAGPRVCLGKEMAFI 60
           MGRM  LWGKD+FEFKP RW+ E DGR      VKLVSPYKFPIFQAGPRVCLGKEMAF+
Sbjct: 384 MGRMVALWGKDQFEFKPSRWVVEQDGRGGRRRGVKLVSPYKFPIFQAGPRVCLGKEMAFL 443

Query: 61  QMKYVVASILGRFRIRPVAVDDPVFVPLLTAHMAGGFRVLFQRRERAGLRE 106
           QMKYVVASIL +FRI+PVA D PVFVPLLTAHMAGGF+V  QRRER   RE
Sbjct: 444 QMKYVVASILSQFRIKPVAGDHPVFVPLLTAHMAGGFKVFIQRRERERERE 494

[Close]

BLAST of Moc08g13720.1 vs. TAIR 10
Match: AT3G48520.1 (cytochrome P450, family 94, subfamily B, polypeptide 3 )

HSP 1 Score: 142.1 bits (357), Expect = 2.6e-34
Identity = 68/102 (66.67%), Postives = 76/102 (74.51%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR----VKLVSPYKFPIFQAGPRVCLGKEMAFIQM 60
           MGRMETLWG D  EF P RW     G     +K +SPYKFP+FQAGPRVC+GKEMAF+QM
Sbjct: 398 MGRMETLWGTDSEEFNPNRWFDSEPGSTRPVLKPISPYKFPVFQAGPRVCVGKEMAFMQM 457

Query: 61  KYVVASILGRFRIRPVAVDDPVFVPLLTAHMAGGFRVLFQRR 99
           KYVV S+L RF I PV  D PVFVPLLTAHMAGG +V  +RR
Sbjct: 458 KYVVGSVLSRFEIVPVNKDRPVFVPLLTAHMAGGLKVKIKRR 499

[Close]

BLAST of Moc08g13720.1 vs. TAIR 10
Match: AT5G63450.1 (cytochrome P450, family 94, subfamily B, polypeptide 1 )

HSP 1 Score: 138.7 bits (348), Expect = 2.9e-33
Identity = 67/105 (63.81%), Postives = 78/105 (74.29%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR-----VKLVSPYKFPIFQAGPRVCLGKEMAFIQ 60
           MGRME +WGKD  EFKP RW  E         +K VS +KFP+FQAGPRVC+GKEMAF Q
Sbjct: 400 MGRMEKVWGKDWDEFKPNRWFEEEPSYGTKPVLKSVSSFKFPVFQAGPRVCIGKEMAFTQ 459

Query: 61  MKYVVASILGRFRIRPVAVDDPVFVPLLTAHMAGGFRVLFQRRER 101
           MKYVV S+L RF+I PV  + PVFVPLLTAHMAGG +V  +RRE+
Sbjct: 460 MKYVVGSVLSRFKIIPVCNNRPVFVPLLTAHMAGGLKVKIKRREQ 504

[Close]

BLAST of Moc08g13720.1 vs. TAIR 10
Match: AT3G01900.1 (cytochrome P450, family 94, subfamily B, polypeptide 2 )

HSP 1 Score: 137.9 bits (346), Expect = 4.9e-33
Identity = 64/103 (62.14%), Postives = 77/103 (74.76%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGR----VKLVSPYKFPIFQAGPRVCLGKEMAFIQM 60
           MGRME LWG+D  EFKP RW    D      +K V+P+KFP+FQAGPRVCLG+EMA++QM
Sbjct: 389 MGRMEELWGEDWDEFKPNRWAESYDKTCCRVLKKVNPFKFPVFQAGPRVCLGEEMAYVQM 448

Query: 61  KYVVASILGRFRIRPVAVDDPVFVPLLTAHMAGGFRVLFQRRE 100
           KY+VASIL RF I P+  D P FVP+LTAHMAGG +V   RR+
Sbjct: 449 KYIVASILDRFEIEPIPTDKPDFVPMLTAHMAGGMQVRVHRRD 491

[Close]

BLAST of Moc08g13720.1 vs. TAIR 10
Match: AT3G56630.1 (cytochrome P450, family 94, subfamily D, polypeptide 2 )

HSP 1 Score: 102.4 bits (254), Expect = 2.3e-22
Identity = 49/99 (49.49%), Postives = 65/99 (65.66%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGRVKLVSPYKFPIFQAGPRVCLGKEMAFIQMKYVV 60
           MGRME++WGKD   F P RW+ E +G  +  +PYKFP F AGPR+CLGKEMA+IQMK +V
Sbjct: 400 MGRMESIWGKDCDRFDPERWIDETNGGFRGENPYKFPAFHAGPRMCLGKEMAYIQMKSIV 459

Query: 61  ASILGRFRIR-PVAVDDPVFVPLLTAHMAGGFRVLFQRR 99
           A++L RF +  P   + P  +  +T  + GG  V  Q R
Sbjct: 460 AAVLERFVVEVPGKKERPEILMSVTLRIRGGLNVRVQER 498

[Close]

BLAST of Moc08g13720.1 vs. TAIR 10
Match: AT2G27690.1 (cytochrome P450, family 94, subfamily C, polypeptide 1 )

HSP 1 Score: 96.3 bits (238), Expect = 1.6e-20
Identity = 47/101 (46.53%), Postives = 65/101 (64.36%), Query Frame = 0


Query: 1   MGRMETLWGKDRFEFKPGRWLAEPDGRVKLVSPYKFPIFQAGPRVCLGKEMAFIQMKYVV 60
           MGRM+ +WG D  EFKP RWL + +G+ +  +P K+P+FQAG RVC+GKEMA ++MK + 
Sbjct: 395 MGRMDRIWGPDYEEFKPERWL-DNEGKFRPENPVKYPVFQAGARVCIGKEMAIMEMKSIA 454

Query: 61  ASILGRFRIR---PVAVDDPVFVPLLTAHMAGGFRVLFQRR 99
            +I+ RF  R   P   +   F P LTA + GG  V+ Q R
Sbjct: 455 VAIIRRFETRVASPETTETLRFAPGLTATVNGGLPVMIQER 494

The following BLAST results are available for this feature:

BLAST of Moc08g13720.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022982702.1	6.5e-43	77.39	cytochrome P450 94B3 [Cucurbita maxima]	[more]
KAG6588901.1	1.1e-42	82.41	Cytochrome P450 94B3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022928304.1	2.5e-42	75.63	cytochrome P450 94B3-like [Cucurbita moschata]	[more]
XP_023529331.1	3.2e-42	76.72	cytochrome P450 94B3-like [Cucurbita pepo subsp. pepo] >XP_023529333.1 cytochrom...	[more]
KAG6581095.1	7.1e-42	82.86	Cytochrome P450 94B1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Moc08g13720.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SMP5	3.7e-33	66.67	Cytochrome P450 94B3 OS=Arabidopsis thaliana OX=3702 GN=CYP94B3 PE=1 SV=1	[more]
Q9FMV7	4.1e-32	63.81	Cytochrome P450 94B1 OS=Arabidopsis thaliana OX=3702 GN=CYP94B1 PE=1 SV=1	[more]
O81117	9.1e-24	50.48	Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2	[more]
P98188	8.5e-22	50.49	Cytochrome P450 94A2 OS=Vicia sativa OX=3908 GN=CYP94A2 PE=2 SV=1	[more]
Q9ZUX1	2.3e-19	46.53	Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1	[more]

BLAST of Moc08g13720.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IXA5	3.1e-43	77.39	cytochrome P450 94B3 OS=Cucurbita maxima OX=3661 GN=LOC111481487 PE=3 SV=1	[more]
A0A6J1EKG0	1.2e-42	75.63	cytochrome P450 94B3-like OS=Cucurbita moschata OX=3662 GN=LOC111435168 PE=3 SV=...	[more]
A0A6J1F3A4	3.5e-42	82.86	cytochrome P450 94B1-like OS=Cucurbita moschata OX=3662 GN=LOC111441980 PE=3 SV=...	[more]
A0A6J1JG29	5.0e-41	76.79	cytochrome P450 94B1-like OS=Cucurbita maxima OX=3661 GN=LOC111486590 PE=4 SV=1	[more]
A0A0A0LBW1	8.5e-41	77.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G585890 PE=3 SV=1	[more]

BLAST of Moc08g13720.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48520.1	2.6e-34	66.67	cytochrome P450, family 94, subfamily B, polypeptide 3	[more]
AT5G63450.1	2.9e-33	63.81	cytochrome P450, family 94, subfamily B, polypeptide 1	[more]
AT3G01900.1	4.9e-33	62.14	cytochrome P450, family 94, subfamily B, polypeptide 2	[more]
AT3G56630.1	2.3e-22	49.49	cytochrome P450, family 94, subfamily D, polypeptide 2	[more]
AT2G27690.1	1.6e-20	46.53	cytochrome P450, family 94, subfamily C, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 1..76 e-value: 4.6E-12 score: 45.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..107 e-value: 8.4E-21 score: 76.0
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 1..98
None	No IPR available	PANTHER	PTHR24296	CYTOCHROME P450	coord: 1..97
None	No IPR available	PANTHER	PTHR24296:SF5	CYTOCHROME P450 94B3	coord: 1..97

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc08g13720	Moc08g13720	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc08g13720.1.exon1	Moc08g13720.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc08g13720.1	cds.Moc08g13720.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc08g13720.1	Moc08g13720.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen