Moc08g05860.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g05860.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	1-aminocyclopropane-1-carboxylate synthase
Location	chr8: 4304862 .. 4306854 (-)
Sequence length	1440
RNA-Seq Expression	Moc08g05860.1
Synteny	Moc08g05860.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAATGTTATCTAGCAAAGCTAGCCATGATTCTCATGGCCAGGACTCCTCCTACTTCTTTGGGTGGCAAGAGTACGAGAAGAACCCCTACCACCCAACTCGTAACCCCACTGGAATTATTCAAATGGGTCTCGCTGAAAACGAGGTAATTTTTAAATTTTGTGTAATACCCACCTCCCAAAATTTGAAATAATTGCATGAAAATTATATATACATATTATTTTGGGTATGGGCAGCTGTCTTTCGACCTGGTGGAGGAGTGGCTGGATAGAAATCCAGATGCCTTGGGATTGAGAAGAAATGGGAAGTCAGTGTTCAGAGAGCTGGCGCTTTTCCAAGACTACCATGGCTTGCCCGCTTTTAAAAAAGTGAGTTACTAATTTCCAATTTCTCGGTCTAAGTTCTTTTTTTCCCCTTAATTTGAATTGAAAAATAATTGTGAGTTATGTTATGTGTGTGTGTAATAATAATTGTGTAGGAGCTGGTGGAGTTGATGGCGGAAATACGAGGAAACAAAGTGAAATTCGATGTCAACAACCTCGTCCTCACCGCTGGTGCAACTTCTGCCAATGAGATCCTTATGTTTTGTCTCGCTCAATCTGGAGAAGCCTTTCTCGTTCCCACCCCATACTATCCAGGGTAACCCCACATTCTAAATAAATAAATAAATAAATAAAAAATCAACTATTTCGTTAAAATCCCACGTTGATACATGAGTTACTCCTCTCACTCTCGATAGTTTTGAAATAAACCTCTATGAAGTATTGATATTTTGTATACAAAACTCTTACTTTTATCTGGAATCCTCTCGCATGGCTGAAAAATATTTATCTTAATTGATAAAATAATCTATCTCGTTAGTTTCAAAAAAAATCAAGCTCATCAGTTAAAAAAAAAAAGTTATATTTTAGTTATCATTTAGCGGTAGAGTATTATCTCAGATTGAAAAAAAAAAAAGAAATTTGAATTTTCTTTTTTTTATGGTTGGCAGGTTTGACAGGGACGTAAAATGGCGAACAGGGGCACAAATAATTCCAATCCAGTGTCCGAGTTCAAACGGATTCCGAATCAGCGGTTGGGCCATGGAGGAGGCGTGGGAACGAGCCGAGGAGCTGAAACTGAGAGTGAAAGGGGTTCTAATAACGAACCCCTCCAACCCATTGGGCACCACAATGGGCCGCGACGAGCTCAATTTACTTGTGGACTTCGCCGCCGCCAAAGGCATCCACATCGTCAGCGACGAGATTTACTCCGCCACCGTCTTCGACTCCCCCTCTTTCATAAGCATCACCGAAGCCCTTATCGATCGGAACCTCCAAAATTCCCCACTTTGGAACCGAATTCATGTCGTCTATAGCCTCTCCAAGGACCTCGGAGTCCCCGGGTTCAGAGTGGGCATGATTTATTCCAACGACCGACATGTCGTCGCAGCGGCCACCAAAATGTCCAGCTTCGGCCTCATCTCGTCGCAGACGCAGTACCTGCTGTCGCGGATGCTGTCGGACCGGGACTTCCGGGGGAATTATATGGACGAGACCAAGAGGCGGATCCGGAAGAGAAAGGGGATGCTTGTTTCGGGGCTCCGGAACGCCGGAATCGGGTGCTTGGACAGCAATTCGGGTCTTTTCTGCTGGGTGGACATGAGGCATCTCTTAAAAAACGCCACTTTCGAGGACGAAATGGAGCTGTGGAGGACCATCTTGTGCCAGGTTGGGCTCAATATCTCCCCCGGTTCGGCTTGCCACTGCTCCGAACCGGGTTGGTTCAGAATGTGCTTTGCCAATATGTCGGAACATACTCTCATGCTCGCGATAAGTCGTCTCAAGACATTCGTCGAATCCTCCTCTTCCGGTGGCGACGCTGATGCCAACGAGGAGTTCTGTCGAAGTAATCAGAAAGTTGTTGGTAGATCATACGGTAGGAAAAGGCTGTTAAGACGACTGCTTGAATCGGGGGTTTGTTCTTTGATGTATACGTGTTTTCCATGCTGA

mRNA sequence

ATGGCAATGTTATCTAGCAAAGCTAGCCATGATTCTCATGGCCAGGACTCCTCCTACTTCTTTGGGTGGCAAGAGTACGAGAAGAACCCCTACCACCCAACTCGTAACCCCACTGGAATTATTCAAATGGGTCTCGCTGAAAACGAGCTGTCTTTCGACCTGGTGGAGGAGTGGCTGGATAGAAATCCAGATGCCTTGGGATTGAGAAGAAATGGGAAGTCAGTGTTCAGAGAGCTGGCGCTTTTCCAAGACTACCATGGCTTGCCCGCTTTTAAAAAAGAGCTGGTGGAGTTGATGGCGGAAATACGAGGAAACAAAGTGAAATTCGATGTCAACAACCTCGTCCTCACCGCTGGTGCAACTTCTGCCAATGAGATCCTTATGTTTTGTCTCGCTCAATCTGGAGAAGCCTTTCTCGTTCCCACCCCATACTATCCAGGGTTTGACAGGGACGTAAAATGGCGAACAGGGGCACAAATAATTCCAATCCAGTGTCCGAGTTCAAACGGATTCCGAATCAGCGGTTGGGCCATGGAGGAGGCGTGGGAACGAGCCGAGGAGCTGAAACTGAGAGTGAAAGGGGTTCTAATAACGAACCCCTCCAACCCATTGGGCACCACAATGGGCCGCGACGAGCTCAATTTACTTGTGGACTTCGCCGCCGCCAAAGGCATCCACATCGTCAGCGACGAGATTTACTCCGCCACCGTCTTCGACTCCCCCTCTTTCATAAGCATCACCGAAGCCCTTATCGATCGGAACCTCCAAAATTCCCCACTTTGGAACCGAATTCATGTCGTCTATAGCCTCTCCAAGGACCTCGGAGTCCCCGGGTTCAGAGTGGGCATGATTTATTCCAACGACCGACATGTCGTCGCAGCGGCCACCAAAATGTCCAGCTTCGGCCTCATCTCGTCGCAGACGCAGTACCTGCTGTCGCGGATGCTGTCGGACCGGGACTTCCGGGGGAATTATATGGACGAGACCAAGAGGCGGATCCGGAAGAGAAAGGGGATGCTTGTTTCGGGGCTCCGGAACGCCGGAATCGGGTGCTTGGACAGCAATTCGGGTCTTTTCTGCTGGGTGGACATGAGGCATCTCTTAAAAAACGCCACTTTCGAGGACGAAATGGAGCTGTGGAGGACCATCTTGTGCCAGGTTGGGCTCAATATCTCCCCCGGTTCGGCTTGCCACTGCTCCGAACCGGGTTGGTTCAGAATGTGCTTTGCCAATATGTCGGAACATACTCTCATGCTCGCGATAAGTCGTCTCAAGACATTCGTCGAATCCTCCTCTTCCGGTGGCGACGCTGATGCCAACGAGGAGTTCTGTCGAAGTAATCAGAAAGTTGTTGGTAGATCATACGGTAGGAAAAGGCTGTTAAGACGACTGCTTGAATCGGGGGTTTGTTCTTTGATGTATACGTGTTTTCCATGCTGA

Coding sequence (CDS)

ATGGCAATGTTATCTAGCAAAGCTAGCCATGATTCTCATGGCCAGGACTCCTCCTACTTCTTTGGGTGGCAAGAGTACGAGAAGAACCCCTACCACCCAACTCGTAACCCCACTGGAATTATTCAAATGGGTCTCGCTGAAAACGAGCTGTCTTTCGACCTGGTGGAGGAGTGGCTGGATAGAAATCCAGATGCCTTGGGATTGAGAAGAAATGGGAAGTCAGTGTTCAGAGAGCTGGCGCTTTTCCAAGACTACCATGGCTTGCCCGCTTTTAAAAAAGAGCTGGTGGAGTTGATGGCGGAAATACGAGGAAACAAAGTGAAATTCGATGTCAACAACCTCGTCCTCACCGCTGGTGCAACTTCTGCCAATGAGATCCTTATGTTTTGTCTCGCTCAATCTGGAGAAGCCTTTCTCGTTCCCACCCCATACTATCCAGGGTTTGACAGGGACGTAAAATGGCGAACAGGGGCACAAATAATTCCAATCCAGTGTCCGAGTTCAAACGGATTCCGAATCAGCGGTTGGGCCATGGAGGAGGCGTGGGAACGAGCCGAGGAGCTGAAACTGAGAGTGAAAGGGGTTCTAATAACGAACCCCTCCAACCCATTGGGCACCACAATGGGCCGCGACGAGCTCAATTTACTTGTGGACTTCGCCGCCGCCAAAGGCATCCACATCGTCAGCGACGAGATTTACTCCGCCACCGTCTTCGACTCCCCCTCTTTCATAAGCATCACCGAAGCCCTTATCGATCGGAACCTCCAAAATTCCCCACTTTGGAACCGAATTCATGTCGTCTATAGCCTCTCCAAGGACCTCGGAGTCCCCGGGTTCAGAGTGGGCATGATTTATTCCAACGACCGACATGTCGTCGCAGCGGCCACCAAAATGTCCAGCTTCGGCCTCATCTCGTCGCAGACGCAGTACCTGCTGTCGCGGATGCTGTCGGACCGGGACTTCCGGGGGAATTATATGGACGAGACCAAGAGGCGGATCCGGAAGAGAAAGGGGATGCTTGTTTCGGGGCTCCGGAACGCCGGAATCGGGTGCTTGGACAGCAATTCGGGTCTTTTCTGCTGGGTGGACATGAGGCATCTCTTAAAAAACGCCACTTTCGAGGACGAAATGGAGCTGTGGAGGACCATCTTGTGCCAGGTTGGGCTCAATATCTCCCCCGGTTCGGCTTGCCACTGCTCCGAACCGGGTTGGTTCAGAATGTGCTTTGCCAATATGTCGGAACATACTCTCATGCTCGCGATAAGTCGTCTCAAGACATTCGTCGAATCCTCCTCTTCCGGTGGCGACGCTGATGCCAACGAGGAGTTCTGTCGAAGTAATCAGAAAGTTGTTGGTAGATCATACGGTAGGAAAAGGCTGTTAAGACGACTGCTTGAATCGGGGGTTTGTTCTTTGATGTATACGTGTTTTCCATGCTGA

Protein sequence

MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLDRNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGATSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEEAWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDSPSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSSFGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFCWVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLAISRLKTFVESSSSGGDADANEEFCRSNQKVVGRSYGRKRLLRRLLESGVCSLMYTCFPC

Homology

[Close]

BLAST of Moc08g05860.1 vs. NCBI nr
Match: AAQ14267.1 (1-aminocyclopropane-1-carboxylate synthase [Momordica charantia])

HSP 1 Score: 983.4 bits (2541), Expect = 6.9e-283
Identity = 479/479 (100.00%), Postives = 479/479 (100.00%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD
Sbjct: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
           RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA
Sbjct: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE
Sbjct: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS
Sbjct: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
           PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS
Sbjct: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC
Sbjct: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA
Sbjct: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420

Query: 421 ISRLKTFVESSSSGGDADANEEFCRSNQKVVGRSYGRKRLLRRLLESGVCSLMYTCFPC 480
           ISRLKTFVESSSSGGDADANEEFCRSNQKVVGRSYGRKRLLRRLLESGVCSLMYTCFPC
Sbjct: 421 ISRLKTFVESSSSGGDADANEEFCRSNQKVVGRSYGRKRLLRRLLESGVCSLMYTCFPC 479

[Close]

BLAST of Moc08g05860.1 vs. NCBI nr
Match: XP_022131648.1 (1-aminocyclopropane-1-carboxylate synthase CMA101-like [Momordica charantia])

HSP 1 Score: 980.7 bits (2534), Expect = 4.5e-282
Identity = 478/479 (99.79%), Postives = 478/479 (99.79%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD
Sbjct: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
           RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA
Sbjct: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE
Sbjct: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS
Sbjct: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
           PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS
Sbjct: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC
Sbjct: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA
Sbjct: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420

Query: 421 ISRLKTFVESSSSGGDADANEEFCRSNQKVVGRSYGRKRLLRRLLESGVCSLMYTCFPC 480
           ISRLKTFVESSSSGGDADANEE CRSNQKVVGRSYGRKRLLRRLLESGVCSLMYTCFPC
Sbjct: 421 ISRLKTFVESSSSGGDADANEEXCRSNQKVVGRSYGRKRLLRRLLESGVCSLMYTCFPC 479

[Close]

BLAST of Moc08g05860.1 vs. NCBI nr
Match: XP_038886265.1 (1-aminocyclopropane-1-carboxylate synthase CMA101-like [Benincasa hispida])

HSP 1 Score: 730.3 bits (1884), Expect = 1.1e-206
Identity = 349/433 (80.60%), Postives = 388/433 (89.61%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           MAMLS+KASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAEN+L +DL++EW++
Sbjct: 2   MAMLSTKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENKLCYDLLDEWME 61

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
            NPDALGL+RNG SVFRELALFQDYHGLPAFKK LVE +AEIRGNKVKFDVN LVLTAGA
Sbjct: 62  NNPDALGLKRNGVSVFRELALFQDYHGLPAFKKVLVESLAEIRGNKVKFDVNKLVLTAGA 121

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANEILMFCLA+ GEAFLVPTPYYPGFDRD+KWRTG QIIPI C SSNGFRI+  AMEE
Sbjct: 122 TSANEILMFCLAEPGEAFLVPTPYYPGFDRDLKWRTGVQIIPIHCSSSNGFRITAAAMEE 181

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           A E+A++LKLRVKGVLITNPSNPLGTT+ R+ELNL+VDFA AK IHIVSDEIYSATVF S
Sbjct: 182 AMEQAQKLKLRVKGVLITNPSNPLGTTLSRNELNLVVDFATAKAIHIVSDEIYSATVFSS 241

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
           P F      ++D+ LQ SP+W+RIHVVYSLSKDLG+PGFRVGMIYSND  VVAAATKMSS
Sbjct: 242 PDF----RTIMDQRLQKSPIWDRIHVVYSLSKDLGLPGFRVGMIYSNDPQVVAAATKMSS 301

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGLISSQTQYL+S++L D  FRGNYM+E K RI KRK ML SGLR AGIGCL  N+GLFC
Sbjct: 302 FGLISSQTQYLVSQILGDAKFRGNYMEEMKTRILKRKAMLESGLRKAGIGCLKGNAGLFC 361

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLLK  +FE+EMELW+ ILC+VGLN+SPGSACHCSEPGWFRMCFANMSEHTL +A
Sbjct: 362 WVDMRHLLKYPSFEEEMELWKRILCEVGLNVSPGSACHCSEPGWFRMCFANMSEHTLTVA 421

Query: 421 ISRLKTFVESSSS 434
           + RLK FV+S++S
Sbjct: 422 MCRLKAFVDSTAS 430

[Close]

BLAST of Moc08g05860.1 vs. NCBI nr
Match: KAG7029285.1 (1-aminocyclopropane-1-carboxylate synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 723.4 bits (1866), Expect = 1.3e-204
Identity = 349/428 (81.54%), Postives = 385/428 (89.95%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           MAMLS+KASHDSHGQDSSYF GWQEYEKNPYHPTRNPTGIIQMGLAENELSFD +E+WL 
Sbjct: 1   MAMLSTKASHDSHGQDSSYFIGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDQLEKWLA 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
            NPDALGLRRNG+SVFRELALFQ+YHGLPAFKK LVELMAEIRGNKVKFDVNNLVLTAGA
Sbjct: 61  NNPDALGLRRNGESVFRELALFQNYHGLPAFKKVLVELMAEIRGNKVKFDVNNLVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANEILMFCLA+ G+AFLVPTPYYPGFDRD+KWRT  QIIPI C SSNGFRI+  AMEE
Sbjct: 121 TSANEILMFCLAELGDAFLVPTPYYPGFDRDLKWRTHVQIIPIHCWSSNGFRITAAAMEE 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           A ERAE+L LRVKGVLITNPSNPLGTTM R+ELNL+VDFA AKGIHIVSDEIYSATV+++
Sbjct: 181 AMERAEKLNLRVKGVLITNPSNPLGTTMSRNELNLVVDFAKAKGIHIVSDEIYSATVYET 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
           P F      ++D +L+ S +W+RIHVVYSLSKDLG+PGFRVGMIYSN+  VVAAATKMSS
Sbjct: 241 PKF----RTIMDDSLKKSSIWDRIHVVYSLSKDLGLPGFRVGMIYSNNPKVVAAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGL+SSQTQYLLS+ML D  FR NYM+ETKRRIRKRKG+L SGL+ AGI CL  N+GLFC
Sbjct: 301 FGLVSSQTQYLLSQMLGDEKFRRNYMEETKRRIRKRKGLLESGLQKAGIRCLKGNAGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLLK+ TFE+EMELW+ IL QVG+N+SPGSACHCSEPGWFRMCFANMSEHTL +A
Sbjct: 361 WVDMRHLLKSGTFEEEMELWKRILSQVGINVSPGSACHCSEPGWFRMCFANMSEHTLRVA 420

Query: 421 ISRLKTFV 429
           + RLK FV
Sbjct: 421 MRRLKAFV 424

[Close]

BLAST of Moc08g05860.1 vs. NCBI nr
Match: XP_022962244.1 (1-aminocyclopropane-1-carboxylate synthase CMA101-like [Cucurbita moschata])

HSP 1 Score: 723.4 bits (1866), Expect = 1.3e-204
Identity = 349/428 (81.54%), Postives = 385/428 (89.95%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           MAMLS+KASHDSHGQDSSYF GWQEYEKNPYHPTRNPTGIIQMGLAENELSFD +E+WL 
Sbjct: 1   MAMLSTKASHDSHGQDSSYFIGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDQLEKWLA 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
            NPDALGLRRNG+SVFRELALFQ+YHGLPAFKK LVELMAEIRGNKVKFDVNNLVLTAGA
Sbjct: 61  NNPDALGLRRNGESVFRELALFQNYHGLPAFKKVLVELMAEIRGNKVKFDVNNLVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANEILMFCLA+ G+AFLVPTPYYPGFDRD+KWRT  QIIPI C SSNGFRI+  AMEE
Sbjct: 121 TSANEILMFCLAELGDAFLVPTPYYPGFDRDLKWRTHVQIIPIHCWSSNGFRITAAAMEE 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           A ERAE+L LRVKGVLITNPSNPLGTTM R+ELNL+VDFA AKGIHIVSDEIYSATV+++
Sbjct: 181 AMERAEKLNLRVKGVLITNPSNPLGTTMSRNELNLVVDFAKAKGIHIVSDEIYSATVYET 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
           P F      ++D +L+ S +W+RIHVVYSLSKDLG+PGFRVGMIYSN+  VVAAATKMSS
Sbjct: 241 PKF----RTIMDDSLKKSSIWDRIHVVYSLSKDLGLPGFRVGMIYSNNPKVVAAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGL+SSQTQYLLS+ML D  FR NYM+ETKRRIRKRKG+L SGL+ AGI CL  N+GLFC
Sbjct: 301 FGLVSSQTQYLLSQMLGDEKFRRNYMEETKRRIRKRKGLLESGLQKAGIRCLKGNAGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLLK+ TFE+EMELW+ IL QVG+N+SPGSACHCSEPGWFRMCFANMSEHTL +A
Sbjct: 361 WVDMRHLLKSGTFEEEMELWKRILSQVGINVSPGSACHCSEPGWFRMCFANMSEHTLTVA 420

Query: 421 ISRLKTFV 429
           + RLK FV
Sbjct: 421 MRRLKAFV 424

[Close]

BLAST of Moc08g05860.1 vs. ExPASy Swiss-Prot
Match: Q00257 (1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN=ACS2 PE=2 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 4.3e-187
Identity = 305/452 (67.48%), Postives = 378/452 (83.63%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           M MLS+KA+ +SHGQDSSYF GW+ YE NP+H T NP GIIQMGLAEN+LSFDL+E WL 
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
           +NPDA   +R+GKS+FRELALFQDYHGLPAFKK LVE MAEIRGNKV F+ NN+VLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANE LMFCLA++G+AFL+PTPYYPGFDRD+KWRTG +I+PI C SSNGF+I+  A+E+
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITQSALEQ 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           A++ A+   LRVKGVL+TNPSNPLGTTM RDELNL+ DF  +KGIH++SDEIYS TVF S
Sbjct: 181 AYKDAQTRNLRVKGVLVTNPSNPLGTTMNRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
           P F+S  E L +R+ ++  +W R+H+VYSLSKDLG+PGFRVG IYSND  VVAAATKMSS
Sbjct: 241 PGFVSAMEVLKERSSEDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGL+SSQTQYLLS MLSD+ F  +Y+ E ++R+++R+ MLVSGL+ AGI CLDSN+GLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTISYISENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLL++  FE E+ELW+ I+ +VGLNISPGS+CHC+EPGWFR+CFANMSE TL LA
Sbjct: 361 WVDMRHLLESDKFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420

Query: 421 ISRLKTFV---ESSSSGGDADANEEFCRSNQK 450
           + RLK+FV    S+++    + + + C++ +K
Sbjct: 421 VRRLKSFVTELRSTTTSNHRNHDNKICKNIKK 452

[Close]

BLAST of Moc08g05860.1 vs. ExPASy Swiss-Prot
Match: Q42881 (1-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum OX=4081 GN=ACS3 PE=1 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 1.2e-184
Identity = 300/449 (66.82%), Postives = 369/449 (82.18%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           M +LS KA+ +SHGQDSSYF GWQEYEKNPY   +NP GIIQMGLAEN+LSFDL+E WL 
Sbjct: 1   MKLLSEKATCNSHGQDSSYFLGWQEYEKNPYDEIQNPKGIIQMGLAENQLSFDLLESWLA 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
           +NPDA G +RNG+S+FRELALFQDYHGLPAFK  + + M+EIRGN+V FD NNLVLTAGA
Sbjct: 61  QNPDAAGFKRNGESIFRELALFQDYHGLPAFKNAMTKFMSEIRGNRVSFDSNNLVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANE LMFCLA  G+AFL+PTPYYPGFDRD+KWRTGA+I+PI C SSNGFRI+  A+EE
Sbjct: 121 TSANETLMFCLANQGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFRITESALEE 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           A+  A++  L+VKGVL+TNPSNPLGTT+ R+EL LL+ F   KGIH++SDEIYS TVF+S
Sbjct: 181 AYLDAKKRNLKVKGVLVTNPSNPLGTTLNRNELELLLTFIDEKGIHLISDEIYSGTVFNS 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
           P F+S+ E LI++N   + +W R+H+VYSLSKDLG+PGFR+G IYSND  VV+AATKMSS
Sbjct: 241 PGFVSVMEVLIEKNYMKTRVWERVHIVYSLSKDLGLPGFRIGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGL+SSQTQYLLS MLSD+ F   Y+ E ++R++KR  MLV GL++AGI CL+SN+GLFC
Sbjct: 301 FGLVSSQTQYLLSCMLSDKKFTKKYISENQKRLKKRHAMLVKGLKSAGINCLESNAGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLL +  F+ EM+LW+ I+  VGLNISPGS+CHC+EPGWFR+CFANMSE TL LA
Sbjct: 361 WVDMRHLLSSNNFDAEMDLWKKIVYDVGLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 ISRLKTFVESSSSGGDADANEEFCRSNQK 450
           + R+K FVES++       N++   +N K
Sbjct: 421 MRRIKDFVESTAPNATNHQNQQQSNANSK 449

[Close]

BLAST of Moc08g05860.1 vs. ExPASy Swiss-Prot
Match: Q9T065 (1-aminocyclopropane-1-carboxylate synthase 8 OS=Arabidopsis thaliana OX=3702 GN=ACS8 PE=1 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 3.3e-179
Identity = 294/432 (68.06%), Postives = 361/432 (83.56%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           M +LS KAS ++HGQDSSYF+GW+EYEKNPY   +NP GIIQMGLAEN+LSFDL+E WL 
Sbjct: 1   MGLLSKKASCNTHGQDSSYFWGWEEYEKNPYDEIKNPDGIIQMGLAENQLSFDLIESWLA 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
           +NPDA   +R G+S+FRELALFQDYHGLP+FK  + + M+E RGN+V F+ N LVLTAGA
Sbjct: 61  KNPDAANFQREGQSIFRELALFQDYHGLPSFKNAMADFMSENRGNRVSFNPNKLVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           T ANE LMFCLA  G+AFL+PTPYYPGFDRD+KWRTGA+I+PIQC S+NGFRI+  A+EE
Sbjct: 121 TPANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCKSANGFRITKVALEE 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           A+E+A++L L+VKGVLITNPSNPLGTT  R ELN L+DF + K IH++SDEIYS TVF +
Sbjct: 181 AYEQAQKLNLKVKGVLITNPSNPLGTTTTRTELNHLLDFISRKKIHLISDEIYSGTVFTN 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
           P FIS+ E L DR L+N+ +++R+H+VYSLSKDLG+PGFRVG+IYSND  VV+AATKMSS
Sbjct: 241 PGFISVMEVLKDRKLENTDVFDRVHIVYSLSKDLGLPGFRVGVIYSNDDFVVSAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGLISSQTQYLLS +LSD+ F  NY++E + R++ R   LVSGL  AGI CL SN+GLFC
Sbjct: 301 FGLISSQTQYLLSALLSDKTFTKNYLEENQIRLKNRHKKLVSGLEAAGIECLKSNAGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLLK+ TFE E+ELW+ I+ +V LNISPGS+CHC+EPGWFR+CFAN+SE TL +A
Sbjct: 361 WVDMRHLLKSNTFEAEIELWKKIVYEVKLNISPGSSCHCNEPGWFRVCFANLSEETLKVA 420

Query: 421 ISRLKTFVESSS 433
           + RLK FV+  S
Sbjct: 421 LDRLKRFVDGPS 432

[Close]

BLAST of Moc08g05860.1 vs. ExPASy Swiss-Prot
Match: P37821 (1-aminocyclopropane-1-carboxylate synthase OS=Malus domestica OX=3750 GN=ACS-1 PE=1 SV=2)

HSP 1 Score: 628.2 bits (1619), Expect = 7.4e-179
Identity = 296/429 (69.00%), Postives = 353/429 (82.28%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           M MLS  A+ +SHGQDSSYF GWQEYEKNPYH   N  GIIQMGLAEN+L FDL+E WL 
Sbjct: 1   MRMLSRNATFNSHGQDSSYFLGWQEYEKNPYHEVHNTNGIIQMGLAENQLCFDLLESWLA 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
           +NP+A   ++NG+S+F ELALFQDYHGLPAFKK +V+ MAEIRGNKV FD N+LVLTAGA
Sbjct: 61  KNPEAAAFKKNGESIFAELALFQDYHGLPAFKKAMVDFMAEIRGNKVTFDPNHLVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANE  +FCLA  GEA L+PTPYYPGFDRD+KWRTG +I+PI C SSNGF+I+  A+EE
Sbjct: 121 TSANETFIFCLADPGEAVLIPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITETALEE 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           A++ AE+  LRVKGVL+TNPSNPLGTTM R+EL LL+ F   KGIH++SDEIYS T F S
Sbjct: 181 AYQEAEKRNLRVKGVLVTNPSNPLGTTMTRNELYLLLSFVEDKGIHLISDEIYSGTAFSS 240

Query: 241 PSFISITEALIDRNL-QNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMS 300
           PSFIS+ E L DRN  +NS +W R+HVVYSLSKDLG+PGFRVG IYSND  VVAAATKMS
Sbjct: 241 PSFISVMEVLKDRNCDENSEVWQRVHVVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMS 300

Query: 301 SFGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLF 360
           SFGL+SSQTQ+LLS MLSD+    NY+ E  +R+++R+  LVSGL+ +GI CL+ N+GLF
Sbjct: 301 SFGLVSSQTQHLLSAMLSDKKLTKNYIAENHKRLKQRQKKLVSGLQKSGISCLNGNAGLF 360

Query: 361 CWVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLML 420
           CWVDMRHLL++ TFE EMELW+ I+ +V LNISPGS+CHC+EPGWFR+CFAN+ E TL L
Sbjct: 361 CWVDMRHLLRSNTFEAEMELWKKIVYEVHLNISPGSSCHCTEPGWFRVCFANLPERTLDL 420

Query: 421 AISRLKTFV 429
           A+ RLK FV
Sbjct: 421 AMQRLKAFV 429

[Close]

BLAST of Moc08g05860.1 vs. ExPASy Swiss-Prot
Match: Q9M2Y8 (1-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=ACS9 PE=1 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 9.0e-177
Identity = 288/433 (66.51%), Postives = 357/433 (82.45%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           M  LS K + ++HGQDSSYF GW+EYEKNPY   +NP GIIQMGLAEN+L FDL+E WL 
Sbjct: 1   MKQLSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLA 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
           +NPDA GL+++G+S+F+ELALFQDYHGLP FKK L E M EIRGN+V FD + +VL AG+
Sbjct: 61  KNPDAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANE LMFCLA+ G+AFL+PTPYYPGFDRD+KWRTGA+I+PI C SSNGF+I+  A+++
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           A+++A++L L+VKGVL+TNPSNPLGT + R ELNLLVDF  +K IH++SDEIYS TVF  
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
             F+S+ + L D+NL+NS +  R+H+VYSLSKDLG+PGFRVG IYSND  VV+AATKMSS
Sbjct: 241 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGL+SSQTQYLLS +LSD+ F   Y+DE ++R++ R+  LVSGL  AGI CL SN+GLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLL   TFE E+ELW+ I+  V LNISPGS+CHC+EPGWFR+CFANMSE TL LA
Sbjct: 361 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 ISRLKTFVESSSS 434
           + RLK +VES+ S
Sbjct: 421 MKRLKEYVESTDS 433

[Close]

BLAST of Moc08g05860.1 vs. ExPASy TrEMBL
Match: Q71T07 (1-aminocyclopropane-1-carboxylate synthase OS=Momordica charantia OX=3673 GN=ACS2 PE=3 SV=1)

HSP 1 Score: 983.4 bits (2541), Expect = 3.3e-283
Identity = 479/479 (100.00%), Postives = 479/479 (100.00%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD
Sbjct: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
           RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA
Sbjct: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE
Sbjct: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS
Sbjct: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
           PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS
Sbjct: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC
Sbjct: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA
Sbjct: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420

Query: 421 ISRLKTFVESSSSGGDADANEEFCRSNQKVVGRSYGRKRLLRRLLESGVCSLMYTCFPC 480
           ISRLKTFVESSSSGGDADANEEFCRSNQKVVGRSYGRKRLLRRLLESGVCSLMYTCFPC
Sbjct: 421 ISRLKTFVESSSSGGDADANEEFCRSNQKVVGRSYGRKRLLRRLLESGVCSLMYTCFPC 479

[Close]

BLAST of Moc08g05860.1 vs. ExPASy TrEMBL
Match: A0A6J1BU08 (1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Momordica charantia OX=3673 GN=LOC111004776 PE=3 SV=1)

HSP 1 Score: 980.7 bits (2534), Expect = 2.2e-282
Identity = 478/479 (99.79%), Postives = 478/479 (99.79%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD
Sbjct: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
           RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA
Sbjct: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE
Sbjct: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS
Sbjct: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
           PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS
Sbjct: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC
Sbjct: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA
Sbjct: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420

Query: 421 ISRLKTFVESSSSGGDADANEEFCRSNQKVVGRSYGRKRLLRRLLESGVCSLMYTCFPC 480
           ISRLKTFVESSSSGGDADANEE CRSNQKVVGRSYGRKRLLRRLLESGVCSLMYTCFPC
Sbjct: 421 ISRLKTFVESSSSGGDADANEEXCRSNQKVVGRSYGRKRLLRRLLESGVCSLMYTCFPC 479

[Close]

BLAST of Moc08g05860.1 vs. ExPASy TrEMBL
Match: A0A6J1HGI6 (1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Cucurbita moschata OX=3662 GN=LOC111462752 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 6.2e-205
Identity = 349/428 (81.54%), Postives = 385/428 (89.95%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           MAMLS+KASHDSHGQDSSYF GWQEYEKNPYHPTRNPTGIIQMGLAENELSFD +E+WL 
Sbjct: 1   MAMLSTKASHDSHGQDSSYFIGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDQLEKWLA 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
            NPDALGLRRNG+SVFRELALFQ+YHGLPAFKK LVELMAEIRGNKVKFDVNNLVLTAGA
Sbjct: 61  NNPDALGLRRNGESVFRELALFQNYHGLPAFKKVLVELMAEIRGNKVKFDVNNLVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANEILMFCLA+ G+AFLVPTPYYPGFDRD+KWRT  QIIPI C SSNGFRI+  AMEE
Sbjct: 121 TSANEILMFCLAELGDAFLVPTPYYPGFDRDLKWRTHVQIIPIHCWSSNGFRITAAAMEE 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           A ERAE+L LRVKGVLITNPSNPLGTTM R+ELNL+VDFA AKGIHIVSDEIYSATV+++
Sbjct: 181 AMERAEKLNLRVKGVLITNPSNPLGTTMSRNELNLVVDFAKAKGIHIVSDEIYSATVYET 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
           P F      ++D +L+ S +W+RIHVVYSLSKDLG+PGFRVGMIYSN+  VVAAATKMSS
Sbjct: 241 PKF----RTIMDDSLKKSSIWDRIHVVYSLSKDLGLPGFRVGMIYSNNPKVVAAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGL+SSQTQYLLS+ML D  FR NYM+ETKRRIRKRKG+L SGL+ AGI CL  N+GLFC
Sbjct: 301 FGLVSSQTQYLLSQMLGDEKFRRNYMEETKRRIRKRKGLLESGLQKAGIRCLKGNAGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLLK+ TFE+EMELW+ IL QVG+N+SPGSACHCSEPGWFRMCFANMSEHTL +A
Sbjct: 361 WVDMRHLLKSGTFEEEMELWKRILSQVGINVSPGSACHCSEPGWFRMCFANMSEHTLTVA 420

Query: 421 ISRLKTFV 429
           + RLK FV
Sbjct: 421 MRRLKAFV 424

[Close]

BLAST of Moc08g05860.1 vs. ExPASy TrEMBL
Match: A0A6J1K348 (1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Cucurbita maxima OX=3661 GN=LOC111491962 PE=3 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.8e-204
Identity = 348/428 (81.31%), Postives = 383/428 (89.49%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           MAMLS+KASHDSHGQDSSYF GWQEYEKNPYHPTRNPTGIIQMGLAENELSFD +E+WL 
Sbjct: 1   MAMLSTKASHDSHGQDSSYFIGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDQLEKWLA 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
            NPDALGLRRNG+SVFRELALFQ+YHGLPAFKK LVELMAEIRGNKVKFDVNNLVLTAGA
Sbjct: 61  NNPDALGLRRNGESVFRELALFQNYHGLPAFKKVLVELMAEIRGNKVKFDVNNLVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANEILMFCLA+ G+AFLVPTPYYPGFDRD+KWRT  QIIPI C SSNGFRI+  AMEE
Sbjct: 121 TSANEILMFCLAELGDAFLVPTPYYPGFDRDLKWRTHVQIIPIHCSSSNGFRITAAAMEE 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           A ERAE+L LRVKGVLITNPSNPLGTTM R ELNL+VDFA AKGIHIVSDEIYSATV+++
Sbjct: 181 AMERAEKLNLRVKGVLITNPSNPLGTTMSRKELNLVVDFAKAKGIHIVSDEIYSATVYET 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
           P F      ++D  L+ S +W+RIHVVYSLSKDLG+PGFRVGMIYSN+  VVAAATKMSS
Sbjct: 241 PKF----RTIMDDGLKKSSIWDRIHVVYSLSKDLGLPGFRVGMIYSNNPKVVAAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGL+SSQTQYLLS+ML D  FR NYM+ETKRRIRKRKG+L SGL+ AGI CL  N+GLFC
Sbjct: 301 FGLVSSQTQYLLSQMLGDEKFRRNYMEETKRRIRKRKGLLESGLQKAGIRCLKGNAGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLLK+ +FE+EMELW+ IL QVG+N+SPGSACHCSEPGWFRMCFANMSEHTL +A
Sbjct: 361 WVDMRHLLKSGSFEEEMELWKRILSQVGINVSPGSACHCSEPGWFRMCFANMSEHTLTVA 420

Query: 421 ISRLKTFV 429
           + RLK FV
Sbjct: 421 MRRLKAFV 424

[Close]

BLAST of Moc08g05860.1 vs. ExPASy TrEMBL
Match: A0A6A1UUK5 (Aminotran_1_2 domain-containing protein OS=Morella rubra OX=262757 GN=CJ030_MR8G001935 PE=3 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 3.9e-191
Identity = 323/463 (69.76%), Postives = 389/463 (84.02%), Query Frame = 0


Query: 3   MLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLDRN 62
           MLS+KAS DSHGQDS YF GW+EYEKNPYH  +NP GIIQMGLAEN+LSFDL+E WL  N
Sbjct: 1   MLSAKASCDSHGQDSFYFLGWEEYEKNPYHEVQNPVGIIQMGLAENQLSFDLLESWLGSN 60

Query: 63  PDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGATS 122
            D LGL+R G SVFRELALFQDYHG PAFK E+V+ MA++RG KV FD NNLVLTAG+TS
Sbjct: 61  SDVLGLKRKGISVFRELALFQDYHGFPAFKNEMVDFMAKLRGYKVAFDPNNLVLTAGSTS 120

Query: 123 ANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEEAW 182
           ANE LMFCLA+ GEAFL+PTPYYPGFDRD+KWRTG +I+PI C SSN FRI+  A+EEA+
Sbjct: 121 ANETLMFCLAEPGEAFLLPTPYYPGFDRDLKWRTGVEIVPIHCSSSNDFRITERALEEAY 180

Query: 183 ERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDSPS 242
           ++AE+L L+VKGVL+TNPSNPLG+TM +DELNLL+DFA  K +H+VSDEIYS TVFDSPS
Sbjct: 181 QQAEKLNLKVKGVLVTNPSNPLGSTMTKDELNLLIDFAVTKKVHVVSDEIYSGTVFDSPS 240

Query: 243 FISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSSFG 302
           FIS+TEA+ +R++  + +WNRIH+VYSLSKDLG+PGFRVGMIYSN++ VVAAATKMSSFG
Sbjct: 241 FISLTEAVTERSIDETDIWNRIHIVYSLSKDLGLPGFRVGMIYSNNQTVVAAATKMSSFG 300

Query: 303 LISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFCWV 362
           L+SSQTQYLLS MLSD+ F   Y++E +RR+++RK MLV+GLR AGIGCL+SN+GLFCWV
Sbjct: 301 LVSSQTQYLLSNMLSDKKFTSKYIEENQRRLKRRKEMLVAGLRGAGIGCLNSNAGLFCWV 360

Query: 363 DMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLAIS 422
           DMRHLL + TFE E ELW+ I+ +VGLNISPGS+CHCSEPGWFRMCFANMS+ TL +A+ 
Sbjct: 361 DMRHLLSSDTFEAEKELWKRIIFEVGLNISPGSSCHCSEPGWFRMCFANMSQETLKVAMR 420

Query: 423 RLKTFVESSSSGGDADANEEFCRSNQKVVGRSYGRKRLLRRLL 466
           R++ FVE SSS   A  N   C ++Q+  GR+  R  L R +L
Sbjct: 421 RIEAFVEFSSSTAAALDN---C-NHQRAAGRNSRRSSLPRWIL 459

[Close]

BLAST of Moc08g05860.1 vs. TAIR 10
Match: AT4G37770.1 (1-amino-cyclopropane-1-carboxylate synthase 8 )

HSP 1 Score: 629.4 bits (1622), Expect = 2.4e-180
Identity = 294/432 (68.06%), Postives = 361/432 (83.56%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           M +LS KAS ++HGQDSSYF+GW+EYEKNPY   +NP GIIQMGLAEN+LSFDL+E WL 
Sbjct: 1   MGLLSKKASCNTHGQDSSYFWGWEEYEKNPYDEIKNPDGIIQMGLAENQLSFDLIESWLA 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
           +NPDA   +R G+S+FRELALFQDYHGLP+FK  + + M+E RGN+V F+ N LVLTAGA
Sbjct: 61  KNPDAANFQREGQSIFRELALFQDYHGLPSFKNAMADFMSENRGNRVSFNPNKLVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           T ANE LMFCLA  G+AFL+PTPYYPGFDRD+KWRTGA+I+PIQC S+NGFRI+  A+EE
Sbjct: 121 TPANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCKSANGFRITKVALEE 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           A+E+A++L L+VKGVLITNPSNPLGTT  R ELN L+DF + K IH++SDEIYS TVF +
Sbjct: 181 AYEQAQKLNLKVKGVLITNPSNPLGTTTTRTELNHLLDFISRKKIHLISDEIYSGTVFTN 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
           P FIS+ E L DR L+N+ +++R+H+VYSLSKDLG+PGFRVG+IYSND  VV+AATKMSS
Sbjct: 241 PGFISVMEVLKDRKLENTDVFDRVHIVYSLSKDLGLPGFRVGVIYSNDDFVVSAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGLISSQTQYLLS +LSD+ F  NY++E + R++ R   LVSGL  AGI CL SN+GLFC
Sbjct: 301 FGLISSQTQYLLSALLSDKTFTKNYLEENQIRLKNRHKKLVSGLEAAGIECLKSNAGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLLK+ TFE E+ELW+ I+ +V LNISPGS+CHC+EPGWFR+CFAN+SE TL +A
Sbjct: 361 WVDMRHLLKSNTFEAEIELWKKIVYEVKLNISPGSSCHCNEPGWFRVCFANLSEETLKVA 420

Query: 421 ISRLKTFVESSS 433
           + RLK FV+  S
Sbjct: 421 LDRLKRFVDGPS 432

[Close]

BLAST of Moc08g05860.1 vs. TAIR 10
Match: AT3G49700.1 (1-aminocyclopropane-1-carboxylate synthase 9 )

HSP 1 Score: 621.3 bits (1601), Expect = 6.4e-178
Identity = 288/433 (66.51%), Postives = 357/433 (82.45%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           M  LS K + ++HGQDSSYF GW+EYEKNPY   +NP GIIQMGLAEN+L FDL+E WL 
Sbjct: 1   MKQLSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLA 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
           +NPDA GL+++G+S+F+ELALFQDYHGLP FKK L E M EIRGN+V FD + +VL AG+
Sbjct: 61  KNPDAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANE LMFCLA+ G+AFL+PTPYYPGFDRD+KWRTGA+I+PI C SSNGF+I+  A+++
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           A+++A++L L+VKGVL+TNPSNPLGT + R ELNLLVDF  +K IH++SDEIYS TVF  
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
             F+S+ + L D+NL+NS +  R+H+VYSLSKDLG+PGFRVG IYSND  VV+AATKMSS
Sbjct: 241 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGL+SSQTQYLLS +LSD+ F   Y+DE ++R++ R+  LVSGL  AGI CL SN+GLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLL   TFE E+ELW+ I+  V LNISPGS+CHC+EPGWFR+CFANMSE TL LA
Sbjct: 361 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 ISRLKTFVESSSS 434
           + RLK +VES+ S
Sbjct: 421 MKRLKEYVESTDS 433

[Close]

BLAST of Moc08g05860.1 vs. TAIR 10
Match: AT5G65800.1 (ACC synthase 5 )

HSP 1 Score: 619.0 bits (1595), Expect = 3.2e-177
Identity = 284/434 (65.44%), Postives = 356/434 (82.03%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           M  LS+K + + HGQDSSYF GW+EYEKNPY   +NP G+IQMGLAEN+L FDL+E WL 
Sbjct: 1   MKQLSTKVTSNGHGQDSSYFLGWEEYEKNPYDEIKNPNGMIQMGLAENQLCFDLIESWLT 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
           +NPDA  L+RNG+S+FRELALFQDYHG+P FKK + E M EIRGN+V FD   +VL AG+
Sbjct: 61  KNPDAASLKRNGQSIFRELALFQDYHGMPEFKKAMAEFMEEIRGNRVTFDPKKIVLAAGS 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANE LMFCLA+ G+AFL+PTPYYPGFDRD+KWRTGA+I+PI C SSNGF+I+  A+++
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDS 240
           A+++A++L L+VKGVL+TNPSNPLGT + R ELNLLVDF  +K IH++SDEIYS T+F  
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTALTRRELNLLVDFITSKNIHLISDEIYSGTMFGF 240

Query: 241 PSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSS 300
             FIS+ + L D+ L+++ +  R+HVVYSLSKDLG+PGFRVG IYSND  +V+AATKMSS
Sbjct: 241 EQFISVMDVLKDKKLEDTEVSKRVHVVYSLSKDLGLPGFRVGAIYSNDEMIVSAATKMSS 300

Query: 301 FGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFC 360
           FGL+SSQTQYLLS +LSD+ F   Y++E ++R++ R+  LVSGL +AGI CL SN+GLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSQYLEENQKRLKSRQRRLVSGLESAGITCLRSNAGLFC 360

Query: 361 WVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLA 420
           WVDMRHLL   TFE E++LW+ I+  V LNISPGS+CHC+EPGWFR+CFANMSE TL LA
Sbjct: 361 WVDMRHLLDTNTFEAELDLWKKIVYNVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 ISRLKTFVESSSSG 435
           + RLKTFVES+  G
Sbjct: 421 LKRLKTFVESTDCG 434

[Close]

BLAST of Moc08g05860.1 vs. TAIR 10
Match: AT4G08040.1 (1-aminocyclopropane-1-carboxylate synthase 11 )

HSP 1 Score: 614.4 bits (1583), Expect = 7.8e-176
Identity = 287/432 (66.44%), Postives = 357/432 (82.64%), Query Frame = 0


Query: 3   MLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLDRN 62
           MLSSK   DSHGQDSSYF GWQEYEKNP+H + N +GI+QMGLAEN+LSFDL+E+WL+ +
Sbjct: 1   MLSSKVVGDSHGQDSSYFLGWQEYEKNPFHESFNTSGIVQMGLAENQLSFDLIEKWLEEH 60

Query: 63  PDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGATS 122
           P+ LGL++N +SVFR+LALFQDYHGLPAFK  + + M +IR NKVKFD N +VLTAG+TS
Sbjct: 61  PEVLGLKKNDESVFRQLALFQDYHGLPAFKDAMAKFMGKIRENKVKFDTNKMVLTAGSTS 120

Query: 123 ANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEEAW 182
           ANE LMFCLA  G+AFL+P PYYPGFDRD+KWRTG +I+PI C SSNG++I+  A+E+A+
Sbjct: 121 ANETLMFCLANPGDAFLIPAPYYPGFDRDLKWRTGVEIVPIHCVSSNGYKITEDALEDAY 180

Query: 183 ERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAAKGIHIVSDEIYSATVFDSPS 242
           ERA +  L VKGVLITNPSNPLGT+  R+EL+LL+ F + K IH+VSDEIYS TVFDSP 
Sbjct: 181 ERALKHNLNVKGVLITNPSNPLGTSTTREELDLLLTFTSTKKIHMVSDEIYSGTVFDSPE 240

Query: 243 FISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMSSFG 302
           F S+ E   D+N+    L  +IHVVYSLSKDLG+PGFRVG+IYSN+  VV+AATKMSSFG
Sbjct: 241 FTSVLEVAKDKNM---GLDGKIHVVYSLSKDLGLPGFRVGLIYSNNEKVVSAATKMSSFG 300

Query: 303 LISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLFCWV 362
           LISSQTQ+LL+ +LSD  F  NY++E K+R+R+RK  LVSGL+ AGI CL SN+GLFCWV
Sbjct: 301 LISSQTQHLLANLLSDERFTTNYLEENKKRLRERKDRLVSGLKEAGISCLKSNAGLFCWV 360

Query: 363 DMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLMLAIS 422
           D+RHLLK+ TFE E  LW  I+C+VGLNISPGS+CHC EPGWFR+CFANMS+ T+ +A+ 
Sbjct: 361 DLRHLLKSNTFEAEHSLWTKIVCEVGLNISPGSSCHCDEPGWFRVCFANMSDQTMEVAMD 420

Query: 423 RLKTFVESSSSG 435
           R+K FV++++ G
Sbjct: 421 RVKGFVDNNNGG 429

[Close]

BLAST of Moc08g05860.1 vs. TAIR 10
Match: AT2G22810.1 (1-aminocyclopropane-1-carboxylate synthase 4 )

HSP 1 Score: 592.4 bits (1526), Expect = 3.2e-169
Identity = 283/434 (65.21%), Postives = 347/434 (79.95%), Query Frame = 0


Query: 1   MAMLSSKASHDSHGQDSSYFFGWQEYEKNPYHPTRNPTGIIQMGLAENELSFDLVEEWLD 60
           M  LS KA+ +SHGQ SSYF GW+EYEKNPY  T+NP GIIQMGLAEN+L FDL+E WL 
Sbjct: 1   MVQLSRKATCNSHGQVSSYFLGWEEYEKNPYDVTKNPQGIIQMGLAENQLCFDLLESWLA 60

Query: 61  RNPDALGLRRNGKSVFRELALFQDYHGLPAFKKELVELMAEIRGNKVKFDVNNLVLTAGA 120
           +N DA   +R+G+SVFRELALFQDYHGL +FK    + M+E RGN+V FD NNLVLTAGA
Sbjct: 61  QNTDAACFKRDGQSVFRELALFQDYHGLSSFKNAFADFMSENRGNRVSFDSNNLVLTAGA 120

Query: 121 TSANEILMFCLAQSGEAFLVPTPYYPGFDRDVKWRTGAQIIPIQCPSSNGFRISGWAMEE 180
           TSANE LMFCLA  G+AFL+PTPYYPGFDRD+KWRTG +I+PIQ  S+NGFRI+  A+EE
Sbjct: 121 TSANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGVEIVPIQSSSTNGFRITKLALEE 180

Query: 181 AWERAEELKLRVKGVLITNPSNPLGTTMGRDELNLLVDFAAA-KGIHIVSDEIYSATVFD 240
           A+E+A++L L VKG+LITNPSNPLGTT  + ELN+L DF    K IH+VSDEIYS TVF+
Sbjct: 181 AYEQAKKLDLNVKGILITNPSNPLGTTTTQTELNILFDFITKNKNIHLVSDEIYSGTVFN 240

Query: 241 SPSFISITEALIDRNLQNSPLWNRIHVVYSLSKDLGVPGFRVGMIYSNDRHVVAAATKMS 300
           S  FIS+ E L +  L+N+ + NR+H+V SLSKDLG+PGFRVG IYSND+ V++AATKMS
Sbjct: 241 SSEFISVMEILKNNQLENTDVLNRVHIVCSLSKDLGLPGFRVGAIYSNDKDVISAATKMS 300

Query: 301 SFGLISSQTQYLLSRMLSDRDFRGNYMDETKRRIRKRKGMLVSGLRNAGIGCLDSNSGLF 360
           SFGL+SSQTQYLLS +LSD+ F  NY+ E ++R++ R+  LV GL   GI CL SN+GLF
Sbjct: 301 SFGLVSSQTQYLLSSLLSDKKFTKNYLRENQKRLKNRQRKLVLGLEAIGIKCLKSNAGLF 360

Query: 361 CWVDMRHLLKNATFEDEMELWRTILCQVGLNISPGSACHCSEPGWFRMCFANMSEHTLML 420
           CWVDMR LL++ TFE EM+LW+ I+ +V LNISPGS+CHC EPGWFR+CFANM + TL L
Sbjct: 361 CWVDMRPLLRSKTFEAEMDLWKKIVYEVKLNISPGSSCHCEEPGWFRVCFANMIDETLKL 420

Query: 421 AISRLKTFVESSSS 434
           A+ RLK  V+  +S
Sbjct: 421 ALKRLKMLVDDENS 434

The following BLAST results are available for this feature:

BLAST of Moc08g05860.1 vs. NCBI nr


Match Name	E-value	Identity	Description
AAQ14267.1	6.9e-283	100.00	1-aminocyclopropane-1-carboxylate synthase [Momordica charantia]	[more]
XP_022131648.1	4.5e-282	99.79	1-aminocyclopropane-1-carboxylate synthase CMA101-like [Momordica charantia]	[more]
XP_038886265.1	1.1e-206	80.60	1-aminocyclopropane-1-carboxylate synthase CMA101-like [Benincasa hispida]	[more]
KAG7029285.1	1.3e-204	81.54	1-aminocyclopropane-1-carboxylate synthase, partial [Cucurbita argyrosperma subs...	[more]
XP_022962244.1	1.3e-204	81.54	1-aminocyclopropane-1-carboxylate synthase CMA101-like [Cucurbita moschata]	[more]

BLAST of Moc08g05860.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q00257	4.3e-187	67.48	1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN...	[more]
Q42881	1.2e-184	66.82	1-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum OX=4081 GN=...	[more]
Q9T065	3.3e-179	68.06	1-aminocyclopropane-1-carboxylate synthase 8 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
P37821	7.4e-179	69.00	1-aminocyclopropane-1-carboxylate synthase OS=Malus domestica OX=3750 GN=ACS-1 P...	[more]
Q9M2Y8	9.0e-177	66.51	1-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Moc08g05860.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q71T07	3.3e-283	100.00	1-aminocyclopropane-1-carboxylate synthase OS=Momordica charantia OX=3673 GN=ACS...	[more]
A0A6J1BU08	2.2e-282	99.79	1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Momordica charantia OX...	[more]
A0A6J1HGI6	6.2e-205	81.54	1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Cucurbita moschata OX=...	[more]
A0A6J1K348	1.8e-204	81.31	1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Cucurbita maxima OX=36...	[more]
A0A6A1UUK5	3.9e-191	69.76	Aminotran_1_2 domain-containing protein OS=Morella rubra OX=262757 GN=CJ030_MR8G...	[more]

BLAST of Moc08g05860.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37770.1	2.4e-180	68.06	1-amino-cyclopropane-1-carboxylate synthase 8	[more]
AT3G49700.1	6.4e-178	66.51	1-aminocyclopropane-1-carboxylate synthase 9	[more]
AT5G65800.1	3.2e-177	65.44	ACC synthase 5	[more]
AT4G08040.1	7.8e-176	66.44	1-aminocyclopropane-1-carboxylate synthase 11	[more]
AT2G22810.1	3.2e-169	65.21	1-aminocyclopropane-1-carboxylate synthase 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00753	ACCSYNTHASE	coord: 295..318 score: 76.78 coord: 189..213 score: 67.21 coord: 43..69 score: 58.11 coord: 76..92 score: 74.54 coord: 225..248 score: 63.87 coord: 113..133 score: 61.2 coord: 135..156 score: 81.0 coord: 263..287 score: 75.41
None	No IPR available	PANTHER	PTHR43795:SF44	1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE 11	coord: 3..440
None	No IPR available	PANTHER	PTHR43795	BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED	coord: 3..440
None	No IPR available	CDD	cd00609	AAT_like	coord: 45..425 e-value: 3.65279E-60 score: 199.105
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 63..318 e-value: 2.9E-157 score: 525.4
IPR004839	Aminotransferase, class I/classII	PFAM	PF00155	Aminotran_1_2	coord: 41..424 e-value: 9.4E-91 score: 304.7
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 29..423 e-value: 2.9E-157 score: 525.4
IPR004838	Aminotransferases, class-I, pyridoxal-phosphate-binding site	PROSITE	PS00105	AA_TRANSFER_CLASS_1	coord: 269..282
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 3..428

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc08g05860	Moc08g05860	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc08g05860.1.exon4	Moc08g05860.1.exon4	exon
Moc08g05860.1.exon3	Moc08g05860.1.exon3	exon
Moc08g05860.1.exon2	Moc08g05860.1.exon2	exon
Moc08g05860.1.exon1	Moc08g05860.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc08g05860.1	cds.Moc08g05860.1_4	CDS
cds.Moc08g05860.1	cds.Moc08g05860.1_3	CDS
cds.Moc08g05860.1	cds.Moc08g05860.1_2	CDS
cds.Moc08g05860.1	cds.Moc08g05860.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc08g05860.1	Moc08g05860.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0006520	cellular amino acid metabolic process
biological_process	GO:1901566	organonitrogen compound biosynthetic process
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0008483	transaminase activity