Moc08g02330.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g02330.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RING-type E3 ubiquitin transferase
Location	chr8: 1578003 .. 1579055 (+)
Sequence length	1053
RNA-Seq Expression	Moc08g02330.1
Synteny	Moc08g02330.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCAAAATTTGTTTGGAAGTCATCGTCTTGATTGCTTCTCATTTTATTTCTTCATACAAAAAGATCAAAATTGGGAGTGGCGCACGCCTAATCTCCCTCTTCCACCACTCCCTTTCTTTCCTCCTTTCTCTCAGAGCTTCCCCTGTTCCATGGCGCTCACAATTAGCAGAAAACTCTTCCCTTCTACAACTAACTGTTCAGGCTGCCCCTACGACTGTCCTTTCGATTGCTACACTTTTCCAGACTTCGACTACTCGCCGCCGCCGCCGCCGCCTCACCAAAATCAAGCCCTTCAACCTTTCATCGTCGTCGTAATCGCTCTCTCCGTCGGGTTCTTCGCCGCTTTGAACTTCTACATGGTCGTCGTAAAGTGCCGTGGCCCCCGGAACTCGGGGGCGGCGGCCTCCCAATCCGACGGCGGAGAGGGAGAGTTACTGGACCGAAATCAAGTGGATCATCCAATTTGGTTCATCAACACCGTCGGCCTGCATCAATCCATCATCAATTCCATTGCGGCGTGCAAATTCAAGCAGGGGGAGGGTCTGATCGAAGGAACAGAGTGCTCTGTTTGTTTGAGCGAATTTCGACAAGGCGAGATGCTTAGATTGCTGCCCAAATGTAGCCATGCCTTTCATATTGGATGTGTAGACACTTGGCTCAGGTCTCACACCACTTGCCCTCTCTGTCGAGCCCTTATACTCACGGATTTGGGTGCGAGGCCCCCCAATTTGGGTTCTGTGAATCAGATCGAGGCTAATTCGGGCGGAAATGAGGAGACCCAGATGGAGAATGGGGAGATTAATGGCGAAGTTGTGGGGGAAAATCAGGCTGTTGTTGCTGTTTCTGGTGGGAGCTTGGAGAACAGGGGAGAAGTTGTTGATGAACAGAGAGAAGTTGTTGTTCAGGTTGGGGAGATTGTTCCAAAAGAGGGGATTACTGAATCTGATCAGAATCATGGAGTTGAAGGAAATGAGATTCTGATGAATAATCAGATGGATTCTTCTTCTGCTGAGTGTGGAGGTAGATCAGTTAAAATAATAGCTACATAA

mRNA sequence

ATGGAGCAAAATTTGTTTGGAAGTCATCGTCTTGATTGCTTCTCATTTTATTTCTTCATACAAAAAGATCAAAATTGGGAGTGGCGCACGCCTAATCTCCCTCTTCCACCACTCCCTTTCTTTCCTCCTTTCTCTCAGAGCTTCCCCTGTTCCATGGCGCTCACAATTAGCAGAAAACTCTTCCCTTCTACAACTAACTGTTCAGGCTGCCCCTACGACTGTCCTTTCGATTGCTACACTTTTCCAGACTTCGACTACTCGCCGCCGCCGCCGCCGCCTCACCAAAATCAAGCCCTTCAACCTTTCATCGTCGTCGTAATCGCTCTCTCCGTCGGGTTCTTCGCCGCTTTGAACTTCTACATGGTCGTCGTAAAGTGCCGTGGCCCCCGGAACTCGGGGGCGGCGGCCTCCCAATCCGACGGCGGAGAGGGAGAGTTACTGGACCGAAATCAAGTGGATCATCCAATTTGGTTCATCAACACCGTCGGCCTGCATCAATCCATCATCAATTCCATTGCGGCGTGCAAATTCAAGCAGGGGGAGGGTCTGATCGAAGGAACAGAGTGCTCTGTTTGTTTGAGCGAATTTCGACAAGGCGAGATGCTTAGATTGCTGCCCAAATGTAGCCATGCCTTTCATATTGGATGTGTAGACACTTGGCTCAGGTCTCACACCACTTGCCCTCTCTGTCGAGCCCTTATACTCACGGATTTGGGTGCGAGGCCCCCCAATTTGGGTTCTGTGAATCAGATCGAGGCTAATTCGGGCGGAAATGAGGAGACCCAGATGGAGAATGGGGAGATTAATGGCGAAGTTGTGGGGGAAAATCAGGCTGTTGTTGCTGTTTCTGGTGGGAGCTTGGAGAACAGGGGAGAAGTTGTTGATGAACAGAGAGAAGTTGTTGTTCAGGTTGGGGAGATTGTTCCAAAAGAGGGGATTACTGAATCTGATCAGAATCATGGAGTTGAAGGAAATGAGATTCTGATGAATAATCAGATGGATTCTTCTTCTGCTGAGTGTGGAGGTAGATCAGTTAAAATAATAGCTACATAA

Coding sequence (CDS)

ATGGAGCAAAATTTGTTTGGAAGTCATCGTCTTGATTGCTTCTCATTTTATTTCTTCATACAAAAAGATCAAAATTGGGAGTGGCGCACGCCTAATCTCCCTCTTCCACCACTCCCTTTCTTTCCTCCTTTCTCTCAGAGCTTCCCCTGTTCCATGGCGCTCACAATTAGCAGAAAACTCTTCCCTTCTACAACTAACTGTTCAGGCTGCCCCTACGACTGTCCTTTCGATTGCTACACTTTTCCAGACTTCGACTACTCGCCGCCGCCGCCGCCGCCTCACCAAAATCAAGCCCTTCAACCTTTCATCGTCGTCGTAATCGCTCTCTCCGTCGGGTTCTTCGCCGCTTTGAACTTCTACATGGTCGTCGTAAAGTGCCGTGGCCCCCGGAACTCGGGGGCGGCGGCCTCCCAATCCGACGGCGGAGAGGGAGAGTTACTGGACCGAAATCAAGTGGATCATCCAATTTGGTTCATCAACACCGTCGGCCTGCATCAATCCATCATCAATTCCATTGCGGCGTGCAAATTCAAGCAGGGGGAGGGTCTGATCGAAGGAACAGAGTGCTCTGTTTGTTTGAGCGAATTTCGACAAGGCGAGATGCTTAGATTGCTGCCCAAATGTAGCCATGCCTTTCATATTGGATGTGTAGACACTTGGCTCAGGTCTCACACCACTTGCCCTCTCTGTCGAGCCCTTATACTCACGGATTTGGGTGCGAGGCCCCCCAATTTGGGTTCTGTGAATCAGATCGAGGCTAATTCGGGCGGAAATGAGGAGACCCAGATGGAGAATGGGGAGATTAATGGCGAAGTTGTGGGGGAAAATCAGGCTGTTGTTGCTGTTTCTGGTGGGAGCTTGGAGAACAGGGGAGAAGTTGTTGATGAACAGAGAGAAGTTGTTGTTCAGGTTGGGGAGATTGTTCCAAAAGAGGGGATTACTGAATCTGATCAGAATCATGGAGTTGAAGGAAATGAGATTCTGATGAATAATCAGATGGATTCTTCTTCTGCTGAGTGTGGAGGTAGATCAGTTAAAATAATAGCTACATAA

Protein sequence

MEQNLFGSHRLDCFSFYFFIQKDQNWEWRTPNLPLPPLPFFPPFSQSFPCSMALTISRKLFPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSVGFFAALNFYMVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGARPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSSAECGGRSVKIIAT

Homology

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BLAST of Moc08g02330.1 vs. NCBI nr
Match: XP_022132045.1 (RING-H2 finger protein ATL54-like [Momordica charantia])

HSP 1 Score: 721.1 bits (1860), Expect = 4.7e-204
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MEQNLFGSHRLDCFSFYFFIQKDQNWEWRTPNLPLPPLPFFPPFSQSFPCSMALTISRKL 60
           MEQNLFGSHRLDCFSFYFFIQKDQNWEWRTPNLPLPPLPFFPPFSQSFPCSMALTISRKL
Sbjct: 1   MEQNLFGSHRLDCFSFYFFIQKDQNWEWRTPNLPLPPLPFFPPFSQSFPCSMALTISRKL 60

Query: 61  FPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSVGFFAALNFY 120
           FPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSVGFFAALNFY
Sbjct: 61  FPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSVGFFAALNFY 120

Query: 121 MVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINSIAACKFKQG 180
           MVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINSIAACKFKQG
Sbjct: 121 MVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINSIAACKFKQG 180

Query: 181 EGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGA 240
           EGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGA
Sbjct: 181 EGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGA 240

Query: 241 RPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRGEVVDEQREV 300
           RPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRGEVVDEQREV
Sbjct: 241 RPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRGEVVDEQREV 300

Query: 301 VVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSSAECGGRSVKIIAT 351
           VVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSSAECGGRSVKIIAT
Sbjct: 301 VVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSSAECGGRSVKIIAT 350

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BLAST of Moc08g02330.1 vs. NCBI nr
Match: XP_038884462.1 (RING-H2 finger protein ATL54-like [Benincasa hispida])

HSP 1 Score: 376.3 bits (965), Expect = 2.8e-100
Identity = 202/306 (66.01%), Postives = 226/306 (73.86%), Query Frame = 0


Query: 52  MALTISRKLFP-------STTNCSGCPYDCPFDCYTFPDFDYSPPPPPP----HQNQALQ 111
           MA+TISRKLFP        TTNCSGCPYDCPFDCY  PDF YS PPPPP    HQ Q   
Sbjct: 1   MAVTISRKLFPVATGTTQPTTNCSGCPYDCPFDCYGIPDFYYSQPPPPPPLADHQKQL-- 60

Query: 112 PFIVVVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPI 171
           P +++++ L VGFF A +FY++VVKCR    G  N G  A QSDGGEGE ++ NQVDHPI
Sbjct: 61  PTLIIILLLFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQVDHPI 120

Query: 172 WFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 231
           WFI T GL QS+INSI  CK+K+ EGLIEGTECSVCLSEF++ EMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 232 VDTWLRSHTTCPLCRALILTDLGA----RPPNLGSVNQIEANSGGNEETQMENGEINGEV 291
           VDTWLR+HTTCPLCRA ILTD  A    RPPNLG VNQ EAN G NEETQMEN EINGEV
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTASNSVRPPNLGPVNQNEANLGVNEETQMENEEINGEV 240

Query: 292 VGENQAVVAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMNNQ 339
           + EN+ V  VS G+ ENRG+VVDEQREV           GI ++D +  VEGNE   NNQ
Sbjct: 241 LVENEGV--VSRGNFENRGDVVDEQREV-----------GIVQTDDSK-VEGNESTRNNQ 290

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BLAST of Moc08g02330.1 vs. NCBI nr
Match: XP_008444871.1 (PREDICTED: RING-H2 finger protein ATL54-like [Cucumis melo] >KAA0065127.1 RING-H2 finger protein ATL54-like [Cucumis melo var. makuwa])

HSP 1 Score: 359.0 bits (920), Expect = 4.7e-95
Identity = 197/309 (63.75%), Postives = 223/309 (72.17%), Query Frame = 0


Query: 52  MALTISRKLFP-------STTNCSGCPYDCPFDCYTFPDFDYSPPPPPP----HQNQALQ 111
           MA+T SRKLFP        TTNCS CPYDCP+DCY FPD  +SPPPPPP    HQ Q   
Sbjct: 1   MAVTHSRKLFPVATGTTQPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPPLFDHQKQL-- 60

Query: 112 PFIVVVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPI 171
           P +++V+ L V FF A  FY++VVKCR    G  N GA A QSDGGEGE ++ NQVDHPI
Sbjct: 61  PTMIIVLILFVAFFIAFTFYIIVVKCRSWYSGSGNEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 172 WFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 231
           WFI T GL QS+INSI  CK+K+ EGLIEGT+CSVCLSEF++ EMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 232 VDTWLRSHTTCPLCRALILTDL------GARPPNLGSVNQIEANSGGNEETQMENGEING 291
           VDTWLR+HTTCPLCRA ILTD         RPPNLG +NQ E N G NE+TQMEN  ING
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTSNSNSIRPPNLGPLNQNEGNLGLNEDTQMENENING 240

Query: 292 EVVGENQ-AVVAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILM 338
           E V EN+  VV++S  S ENRG+VVDEQRE    V EIV KEG  ESD +  VEGNE   
Sbjct: 241 EAVRENEGGVVSISSESSENRGDVVDEQRE----VEEIVQKEGNFESDDSK-VEGNESTR 300

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BLAST of Moc08g02330.1 vs. NCBI nr
Match: XP_011649673.1 (RING-H2 finger protein ATL54 [Cucumis sativus] >KGN62717.1 hypothetical protein Csa_022380 [Cucumis sativus])

HSP 1 Score: 355.5 bits (911), Expect = 5.2e-94
Identity = 191/307 (62.21%), Postives = 221/307 (71.99%), Query Frame = 0


Query: 52  MALTISRKLFP-------STTNCSGCPYDCPFDCYTFPDFDYSPPPPPP----HQNQALQ 111
           MA+T+SRKLFP        TTNCS CPYDCP+DCY FPD  +SPPPPPP    HQ Q   
Sbjct: 1   MAVTLSRKLFPVATGTALPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPPLSDHQKQL-- 60

Query: 112 PFIVVVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPI 171
           P  ++++ L + FF A  FY++VVKCR    G  N GA A QSDGGEGE ++ NQVDHPI
Sbjct: 61  PTTIIILILFIAFFVAFTFYIIVVKCRSWYSGSANEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 172 WFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 231
           WFI T GL QS+INSI  CK+K+ EGLIEGT+CSVCLSEF++ EMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 232 VDTWLRSHTTCPLCRALILTDL----GARPPNLGSVNQIEANSGGNEETQMENGEINGEV 291
           VDTWLR+HTTCPLCRA ILTD       RPPN+G +NQ E N G NE+TQMEN   N E 
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTPNSVRPPNIGPLNQNEGNLGLNEDTQMENENTNREA 240

Query: 292 VGENQ-AVVAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMN- 338
           V EN+   V++S  S ENRG+ VDEQRE    V EIV KEG  ESD +  VEGNE + N 
Sbjct: 241 VRENEGGGVSISSESSENRGDAVDEQRE----VEEIVQKEGNFESDDSK-VEGNESMRND 300

[Close]

BLAST of Moc08g02330.1 vs. NCBI nr
Match: XP_022997232.1 (RING-H2 finger protein ATL54-like [Cucurbita maxima])

HSP 1 Score: 339.3 bits (869), Expect = 3.8e-89
Identity = 182/295 (61.69%), Postives = 213/295 (72.20%), Query Frame = 0


Query: 52  MALTISRKLFPSTT-------NCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIV 111
           MA+ I+RKLFP  T       NCSGCPYDCPFDC   PDF +SPPPP     + L   ++
Sbjct: 1   MAVGITRKLFPVETGTHQQSFNCSGCPYDCPFDCLDLPDFYFSPPPPFGDHQKQLPTIVI 60

Query: 112 VVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPIWFIN 171
           V++AL VGFF   +FY+++VK +    G  N GA ASQ DG +GE L+ NQVDHPIWFI 
Sbjct: 61  VILALFVGFFFTFSFYIIIVKWQAWRSGSGNQGAEASQLDGEDGEFLNENQVDHPIWFIT 120

Query: 172 TVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTW 231
           TVGL QSIINSI  CK+K+GEGLIEGT CSVCL+EF++ EMLRLLPKCSHAFHIGCVDTW
Sbjct: 121 TVGLQQSIINSITVCKYKKGEGLIEGTVCSVCLNEFQEDEMLRLLPKCSHAFHIGCVDTW 180

Query: 232 LRSHTTCPLCRALILTDLGA----RPPNLGSVNQIEANSGGNEETQMENGEINGEVVGEN 291
           LR+HTTCPLCRA ILTD       RPPN G VNQ EAN    EETQMEN E      GE 
Sbjct: 181 LRTHTTCPLCRAHILTDFATSNFPRPPNSGHVNQNEANLAVIEETQMENLE------GEE 240

Query: 292 QAVVAVSGGSLENRGEVVDEQREV-VVQVGEIVPKEGITESDQNHGVEGNEILMN 331
           Q+ V+  GG +ENRG+ VDEQR+V V Q+GEIVPKEG+ ESD +  +E NE +MN
Sbjct: 241 QSAVS-GGGGVENRGDAVDEQRDVGVPQIGEIVPKEGVFESDDSK-MEVNETMMN 287

[Close]

BLAST of Moc08g02330.1 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 164.1 bits (414), Expect = 2.9e-39
Identity = 107/263 (40.68%), Postives = 129/263 (49.05%), Query Frame = 0


Query: 52  MALTISRKLFPS-------TTNCSG--CPYDCPFDCYTFPD-FDYSPPPPP----PHQNQ 111
           MA    RKLFP+       T +CS   C   CP++CY  PD +  SP  PP    P  + 
Sbjct: 1   MARKKHRKLFPTLASETNKTLDCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSP 60

Query: 112 ALQPFI------------------------VVVIALSVGFFAALNFYMVVVKCRGPRNSG 171
              P I                         V+  L  GFF    F+   V      N G
Sbjct: 61  CPSPSISAVYLPSQDSSSSLDAISIITITGAVLAILLTGFFLVAKFFSDSV---NRVNQG 120

Query: 172 AAASQSDGG----EGELLDRNQVDHPIWFINTVGLHQSIINSIAACKFKQGEGLIEGTEC 231
              S ++      E E  DR QVDHPIW I T GL QSIINSI  C +K+G+GLIE T+C
Sbjct: 121 TYQSDNEDNDTVMEEEFQDREQVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDC 180

Query: 232 SVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGARPPNLGSVN 273
            VCL+EF + E LRLLPKC+HAFHI C+DTWL SHT CPLCRA I       P   G V 
Sbjct: 181 PVCLNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVE 240

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BLAST of Moc08g02330.1 vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.3e-35
Identity = 80/162 (49.38%), Postives = 103/162 (63.58%), Query Frame = 0


Query: 88  PPPPPPHQNQALQPFIVVVIALSVGFFAALNFYMVVVKCRGPRNSGAAASQSDGG----E 147
           PPPPPP +   L P  + V+  S+  F  L F++ +   +  R S A+ +  D      E
Sbjct: 30  PPPPPPRELSLLLPTSICVVG-SIILFLFLVFFLYLHITQQRRISAASVTPGDTNQQEDE 89

Query: 148 GELLDRNQVD-HPIWFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEML 207
            E  +R+  D H +W I TVGLH+S INSI    FK+GEG+I+GTECSVCL+EF + E L
Sbjct: 90  DETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECSVCLNEFEEDESL 149

Query: 208 RLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGARPPN 245
           RLLPKCSHAFH+ C+DTWL SH  CPLCRA +L  L   PP+
Sbjct: 150 RLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVL--LITEPPH 188

[Close]

BLAST of Moc08g02330.1 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 126.7 bits (317), Expect = 5.1e-28
Identity = 87/264 (32.95%), Postives = 120/264 (45.45%), Query Frame = 0


Query: 61  FPSTTNCSG--CPYDCPFDCYTFPDFDYSPPPP--------PPHQNQALQPFIVVVIALS 120
           +  T +CS   C   CP  CY    F + PPPP                 P ++ +I + 
Sbjct: 11  YEPTRDCSQGLCSMYCPQWCY----FIFPPPPPFDVAGTSADDSSGPVFSPLVIAIIGVL 70

Query: 121 VGFFAALNFYMVVVKCRGP----------RNSGAAASQSDGGEGELLDRNQV-DHPIWFI 180
              F  +++Y  + K  G            +SG AA     G G      Q   H  W +
Sbjct: 71  ASAFLLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRHGHGQSRSHESWNV 130

Query: 181 N-TVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVD 240
           +   GL +++IN I  CK+++G+G +  T+CSVCL EF  GE LRLLP+CSHAFH  C+D
Sbjct: 131 SPPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHAFHQQCID 190

Query: 241 TWLRSHTTCPLCRA-LILTDLGARPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQ 300
           TWL+SH+ CPLCRA +    +G   P                      G   GE  G+N 
Sbjct: 191 TWLKSHSNCPLCRANITFVTVGLASPE-------------------PEGCAPGETGGDNT 248

Query: 301 AVVAVSGGSLENRGEVVDEQREVV 302
             V V    LEN   + +EQ+E V
Sbjct: 251 HEVVVVMDGLEN---LCEEQQEAV 248

[Close]

BLAST of Moc08g02330.1 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.5e-27
Identity = 76/228 (33.33%), Postives = 118/228 (51.75%), Query Frame = 0


Query: 61  FPSTTNCSG--CPYDCPFDCYTFPDFDYSPPPPP--------PHQNQALQPFIVVVIALS 120
           + S  +CS   C   CP  CY        PPPPP           + +  P ++ +I + 
Sbjct: 11  YDSYNDCSQGICNIYCPQWCYLI-----FPPPPPSFFLDDDSSSSSSSFSPLLIALIGIL 70

Query: 121 VGFFAALNFYMVVVK-C--RGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQS 180
                 +++Y ++ K C      +S    + +  GEG      ++       N  GL++S
Sbjct: 71  TSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQRIS-----TNGDGLNES 130

Query: 181 IINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTC 240
           +I SI   K+K G+G ++G++CSVCLSEF + E LRLLPKC+HAFH+ C+DTWL+SH+ C
Sbjct: 131 MIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNC 190

Query: 241 PLCRALILTDLGARPPNLG-SVNQIEANSGGNEETQMENGEINGEVVG 275
           PLCRA + T +     ++G +V+ + AN   +        EIN  + G
Sbjct: 191 PLCRAFV-TGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAG 227

[Close]

BLAST of Moc08g02330.1 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 113.2 bits (282), Expect = 5.8e-24
Identity = 77/247 (31.17%), Postives = 119/247 (48.18%), Query Frame = 0


Query: 61  FPSTTNCSG--CPYDCPFDCYTFPDFDYSPPPP-------PPHQNQALQPFIVVVIALSV 120
           + S  +CS   C   CP  CY        PPPP           +    P ++ +I +  
Sbjct: 14  YDSYRDCSQGVCSVYCPQWCYVI-----FPPPPSFYLDDEDDSSSSDFSPLLIALIGILA 73

Query: 121 GFFAALNFYMVVVK-CRGPRNSGAAASQS-----------DGGEGELLDRNQVDHPIWFI 180
             F  +++Y ++ K C   R++ ++ S +            G      + N   +P   I
Sbjct: 74  SAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNN--NNNGATNPNQTI 133

Query: 181 ---NTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 240
                 GL +S+I SI   K+++ +G +E ++CSVCLSEF++ E LRLLPKC+HAFH+ C
Sbjct: 134 GGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPC 193

Query: 241 VDTWLRSHTTCPLCRALILTDLGARPPNLGSVNQIEAN---SGGNEET-----QMENGEI 276
           +DTWL+SH+ CPLCRA I+T       +L +   +  N   S G++        +EN   
Sbjct: 194 IDTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGDDSVVVVNLDLENSRS 253

[Close]

BLAST of Moc08g02330.1 vs. ExPASy TrEMBL
Match: A0A6J1BRC8 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111005011 PE=3 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 2.3e-204
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MEQNLFGSHRLDCFSFYFFIQKDQNWEWRTPNLPLPPLPFFPPFSQSFPCSMALTISRKL 60
           MEQNLFGSHRLDCFSFYFFIQKDQNWEWRTPNLPLPPLPFFPPFSQSFPCSMALTISRKL
Sbjct: 1   MEQNLFGSHRLDCFSFYFFIQKDQNWEWRTPNLPLPPLPFFPPFSQSFPCSMALTISRKL 60

Query: 61  FPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSVGFFAALNFY 120
           FPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSVGFFAALNFY
Sbjct: 61  FPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSVGFFAALNFY 120

Query: 121 MVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINSIAACKFKQG 180
           MVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINSIAACKFKQG
Sbjct: 121 MVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINSIAACKFKQG 180

Query: 181 EGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGA 240
           EGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGA
Sbjct: 181 EGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGA 240

Query: 241 RPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRGEVVDEQREV 300
           RPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRGEVVDEQREV
Sbjct: 241 RPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRGEVVDEQREV 300

Query: 301 VVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSSAECGGRSVKIIAT 351
           VVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSSAECGGRSVKIIAT
Sbjct: 301 VVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSSAECGGRSVKIIAT 350

[Close]

BLAST of Moc08g02330.1 vs. ExPASy TrEMBL
Match: A0A5A7VDK2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004620 PE=3 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 2.3e-95
Identity = 197/309 (63.75%), Postives = 223/309 (72.17%), Query Frame = 0


Query: 52  MALTISRKLFP-------STTNCSGCPYDCPFDCYTFPDFDYSPPPPPP----HQNQALQ 111
           MA+T SRKLFP        TTNCS CPYDCP+DCY FPD  +SPPPPPP    HQ Q   
Sbjct: 1   MAVTHSRKLFPVATGTTQPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPPLFDHQKQL-- 60

Query: 112 PFIVVVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPI 171
           P +++V+ L V FF A  FY++VVKCR    G  N GA A QSDGGEGE ++ NQVDHPI
Sbjct: 61  PTMIIVLILFVAFFIAFTFYIIVVKCRSWYSGSGNEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 172 WFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 231
           WFI T GL QS+INSI  CK+K+ EGLIEGT+CSVCLSEF++ EMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 232 VDTWLRSHTTCPLCRALILTDL------GARPPNLGSVNQIEANSGGNEETQMENGEING 291
           VDTWLR+HTTCPLCRA ILTD         RPPNLG +NQ E N G NE+TQMEN  ING
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTSNSNSIRPPNLGPLNQNEGNLGLNEDTQMENENING 240

Query: 292 EVVGENQ-AVVAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILM 338
           E V EN+  VV++S  S ENRG+VVDEQRE    V EIV KEG  ESD +  VEGNE   
Sbjct: 241 EAVRENEGGVVSISSESSENRGDVVDEQRE----VEEIVQKEGNFESDDSK-VEGNESTR 300

[Close]

BLAST of Moc08g02330.1 vs. ExPASy TrEMBL
Match: A0A1S3BAW8 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103488086 PE=3 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 2.3e-95
Identity = 197/309 (63.75%), Postives = 223/309 (72.17%), Query Frame = 0


Query: 52  MALTISRKLFP-------STTNCSGCPYDCPFDCYTFPDFDYSPPPPPP----HQNQALQ 111
           MA+T SRKLFP        TTNCS CPYDCP+DCY FPD  +SPPPPPP    HQ Q   
Sbjct: 1   MAVTHSRKLFPVATGTTQPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPPLFDHQKQL-- 60

Query: 112 PFIVVVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPI 171
           P +++V+ L V FF A  FY++VVKCR    G  N GA A QSDGGEGE ++ NQVDHPI
Sbjct: 61  PTMIIVLILFVAFFIAFTFYIIVVKCRSWYSGSGNEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 172 WFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 231
           WFI T GL QS+INSI  CK+K+ EGLIEGT+CSVCLSEF++ EMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 232 VDTWLRSHTTCPLCRALILTDL------GARPPNLGSVNQIEANSGGNEETQMENGEING 291
           VDTWLR+HTTCPLCRA ILTD         RPPNLG +NQ E N G NE+TQMEN  ING
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTSNSNSIRPPNLGPLNQNEGNLGLNEDTQMENENING 240

Query: 292 EVVGENQ-AVVAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILM 338
           E V EN+  VV++S  S ENRG+VVDEQRE    V EIV KEG  ESD +  VEGNE   
Sbjct: 241 EAVRENEGGVVSISSESSENRGDVVDEQRE----VEEIVQKEGNFESDDSK-VEGNESTR 300

[Close]

BLAST of Moc08g02330.1 vs. ExPASy TrEMBL
Match: A0A0A0LPJ4 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G369190 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 2.5e-94
Identity = 191/307 (62.21%), Postives = 221/307 (71.99%), Query Frame = 0


Query: 52  MALTISRKLFP-------STTNCSGCPYDCPFDCYTFPDFDYSPPPPPP----HQNQALQ 111
           MA+T+SRKLFP        TTNCS CPYDCP+DCY FPD  +SPPPPPP    HQ Q   
Sbjct: 1   MAVTLSRKLFPVATGTALPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPPLSDHQKQL-- 60

Query: 112 PFIVVVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPI 171
           P  ++++ L + FF A  FY++VVKCR    G  N GA A QSDGGEGE ++ NQVDHPI
Sbjct: 61  PTTIIILILFIAFFVAFTFYIIVVKCRSWYSGSANEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 172 WFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 231
           WFI T GL QS+INSI  CK+K+ EGLIEGT+CSVCLSEF++ EMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 232 VDTWLRSHTTCPLCRALILTDL----GARPPNLGSVNQIEANSGGNEETQMENGEINGEV 291
           VDTWLR+HTTCPLCRA ILTD       RPPN+G +NQ E N G NE+TQMEN   N E 
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTPNSVRPPNIGPLNQNEGNLGLNEDTQMENENTNREA 240

Query: 292 VGENQ-AVVAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMN- 338
           V EN+   V++S  S ENRG+ VDEQRE    V EIV KEG  ESD +  VEGNE + N 
Sbjct: 241 VRENEGGGVSISSESSENRGDAVDEQRE----VEEIVQKEGNFESDDSK-VEGNESMRND 300

[Close]

BLAST of Moc08g02330.1 vs. ExPASy TrEMBL
Match: A0A6J1K4G1 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492196 PE=3 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 1.9e-89
Identity = 182/295 (61.69%), Postives = 213/295 (72.20%), Query Frame = 0


Query: 52  MALTISRKLFPSTT-------NCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIV 111
           MA+ I+RKLFP  T       NCSGCPYDCPFDC   PDF +SPPPP     + L   ++
Sbjct: 1   MAVGITRKLFPVETGTHQQSFNCSGCPYDCPFDCLDLPDFYFSPPPPFGDHQKQLPTIVI 60

Query: 112 VVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPIWFIN 171
           V++AL VGFF   +FY+++VK +    G  N GA ASQ DG +GE L+ NQVDHPIWFI 
Sbjct: 61  VILALFVGFFFTFSFYIIIVKWQAWRSGSGNQGAEASQLDGEDGEFLNENQVDHPIWFIT 120

Query: 172 TVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTW 231
           TVGL QSIINSI  CK+K+GEGLIEGT CSVCL+EF++ EMLRLLPKCSHAFHIGCVDTW
Sbjct: 121 TVGLQQSIINSITVCKYKKGEGLIEGTVCSVCLNEFQEDEMLRLLPKCSHAFHIGCVDTW 180

Query: 232 LRSHTTCPLCRALILTDLGA----RPPNLGSVNQIEANSGGNEETQMENGEINGEVVGEN 291
           LR+HTTCPLCRA ILTD       RPPN G VNQ EAN    EETQMEN E      GE 
Sbjct: 181 LRTHTTCPLCRAHILTDFATSNFPRPPNSGHVNQNEANLAVIEETQMENLE------GEE 240

Query: 292 QAVVAVSGGSLENRGEVVDEQREV-VVQVGEIVPKEGITESDQNHGVEGNEILMN 331
           Q+ V+  GG +ENRG+ VDEQR+V V Q+GEIVPKEG+ ESD +  +E NE +MN
Sbjct: 241 QSAVS-GGGGVENRGDAVDEQRDVGVPQIGEIVPKEGVFESDDSK-MEVNETMMN 287

[Close]

BLAST of Moc08g02330.1 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 164.1 bits (414), Expect = 2.0e-40
Identity = 107/263 (40.68%), Postives = 129/263 (49.05%), Query Frame = 0


Query: 52  MALTISRKLFPS-------TTNCSG--CPYDCPFDCYTFPD-FDYSPPPPP----PHQNQ 111
           MA    RKLFP+       T +CS   C   CP++CY  PD +  SP  PP    P  + 
Sbjct: 1   MARKKHRKLFPTLASETNKTLDCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSP 60

Query: 112 ALQPFI------------------------VVVIALSVGFFAALNFYMVVVKCRGPRNSG 171
              P I                         V+  L  GFF    F+   V      N G
Sbjct: 61  CPSPSISAVYLPSQDSSSSLDAISIITITGAVLAILLTGFFLVAKFFSDSV---NRVNQG 120

Query: 172 AAASQSDGG----EGELLDRNQVDHPIWFINTVGLHQSIINSIAACKFKQGEGLIEGTEC 231
              S ++      E E  DR QVDHPIW I T GL QSIINSI  C +K+G+GLIE T+C
Sbjct: 121 TYQSDNEDNDTVMEEEFQDREQVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDC 180

Query: 232 SVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGARPPNLGSVN 273
            VCL+EF + E LRLLPKC+HAFHI C+DTWL SHT CPLCRA I       P   G V 
Sbjct: 181 PVCLNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVE 240

[Close]

BLAST of Moc08g02330.1 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 150.2 bits (378), Expect = 3.1e-36
Identity = 80/162 (49.38%), Postives = 103/162 (63.58%), Query Frame = 0


Query: 88  PPPPPPHQNQALQPFIVVVIALSVGFFAALNFYMVVVKCRGPRNSGAAASQSDGG----E 147
           PPPPPP +   L P  + V+  S+  F  L F++ +   +  R S A+ +  D      E
Sbjct: 30  PPPPPPRELSLLLPTSICVVG-SIILFLFLVFFLYLHITQQRRISAASVTPGDTNQQEDE 89

Query: 148 GELLDRNQVD-HPIWFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEML 207
            E  +R+  D H +W I TVGLH+S INSI    FK+GEG+I+GTECSVCL+EF + E L
Sbjct: 90  DETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECSVCLNEFEEDESL 149

Query: 208 RLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGARPPN 245
           RLLPKCSHAFH+ C+DTWL SH  CPLCRA +L  L   PP+
Sbjct: 150 RLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVL--LITEPPH 188

[Close]

BLAST of Moc08g02330.1 vs. TAIR 10
Match: AT4G33565.1 (RING/U-box superfamily protein )

HSP 1 Score: 134.4 bits (337), Expect = 1.7e-31
Identity = 63/124 (50.81%), Postives = 84/124 (67.74%), Query Frame = 0


Query: 152 VDHPIWFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHA 211
           VDHPIW I T+GL+ ++I+SI  C++ + +G++EGT+CSVCLSEF + E LRLLPKC HA
Sbjct: 180 VDHPIWHIRTIGLNPTVISSIKVCQYSKKDGVVEGTDCSVCLSEFEEEETLRLLPKCKHA 239

Query: 212 FHIGCVDTWLRSHTTCPLCRALILTDLGARPPNLGSVNQIEANSGGNEETQMENGEINGE 271
           FH+ C+DTWLRSHT CPLCRA          P + +   I+ +S G EE  +   E NG+
Sbjct: 240 FHLYCIDTWLRSHTNCPLCRA----------PIVEANTMIDDHSEGLEEISVMIPEENGD 293

Query: 272 VVGE 276
              E
Sbjct: 300 DTDE 293

[Close]

BLAST of Moc08g02330.1 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 1.8e-28
Identity = 76/228 (33.33%), Postives = 118/228 (51.75%), Query Frame = 0


Query: 61  FPSTTNCSG--CPYDCPFDCYTFPDFDYSPPPPP--------PHQNQALQPFIVVVIALS 120
           + S  +CS   C   CP  CY        PPPPP           + +  P ++ +I + 
Sbjct: 11  YDSYNDCSQGICNIYCPQWCYLI-----FPPPPPSFFLDDDSSSSSSSFSPLLIALIGIL 70

Query: 121 VGFFAALNFYMVVVK-C--RGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQS 180
                 +++Y ++ K C      +S    + +  GEG      ++       N  GL++S
Sbjct: 71  TSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQRIS-----TNGDGLNES 130

Query: 181 IINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTC 240
           +I SI   K+K G+G ++G++CSVCLSEF + E LRLLPKC+HAFH+ C+DTWL+SH+ C
Sbjct: 131 MIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNC 190

Query: 241 PLCRALILTDLGARPPNLG-SVNQIEANSGGNEETQMENGEINGEVVG 275
           PLCRA + T +     ++G +V+ + AN   +        EIN  + G
Sbjct: 191 PLCRAFV-TGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAG 227

[Close]

BLAST of Moc08g02330.1 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 4.1e-25
Identity = 77/247 (31.17%), Postives = 119/247 (48.18%), Query Frame = 0


Query: 61  FPSTTNCSG--CPYDCPFDCYTFPDFDYSPPPP-------PPHQNQALQPFIVVVIALSV 120
           + S  +CS   C   CP  CY        PPPP           +    P ++ +I +  
Sbjct: 14  YDSYRDCSQGVCSVYCPQWCYVI-----FPPPPSFYLDDEDDSSSSDFSPLLIALIGILA 73

Query: 121 GFFAALNFYMVVVK-CRGPRNSGAAASQS-----------DGGEGELLDRNQVDHPIWFI 180
             F  +++Y ++ K C   R++ ++ S +            G      + N   +P   I
Sbjct: 74  SAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNN--NNNGATNPNQTI 133

Query: 181 ---NTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 240
                 GL +S+I SI   K+++ +G +E ++CSVCLSEF++ E LRLLPKC+HAFH+ C
Sbjct: 134 GGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPC 193

Query: 241 VDTWLRSHTTCPLCRALILTDLGARPPNLGSVNQIEAN---SGGNEET-----QMENGEI 276
           +DTWL+SH+ CPLCRA I+T       +L +   +  N   S G++        +EN   
Sbjct: 194 IDTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGDDSVVVVNLDLENSRS 253

The following BLAST results are available for this feature:

BLAST of Moc08g02330.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132045.1	4.7e-204	100.00	RING-H2 finger protein ATL54-like [Momordica charantia]	[more]
XP_038884462.1	2.8e-100	66.01	RING-H2 finger protein ATL54-like [Benincasa hispida]	[more]
XP_008444871.1	4.7e-95	63.75	PREDICTED: RING-H2 finger protein ATL54-like [Cucumis melo] >KAA0065127.1 RING-H...	[more]
XP_011649673.1	5.2e-94	62.21	RING-H2 finger protein ATL54 [Cucumis sativus] >KGN62717.1 hypothetical protein ...	[more]
XP_022997232.1	3.8e-89	61.69	RING-H2 finger protein ATL54-like [Cucurbita maxima]	[more]

BLAST of Moc08g02330.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LFY8	2.9e-39	40.68	RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2	[more]
Q9LX93	4.3e-35	49.38	E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ...	[more]
Q7XLY8	5.1e-28	32.95	E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q9LF64	2.5e-27	33.33	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]
Q9SRQ8	5.8e-24	31.17	RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2	[more]

BLAST of Moc08g02330.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BRC8	2.3e-204	100.00	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11100501...	[more]
A0A5A7VDK2	2.3e-95	63.75	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A1S3BAW8	2.3e-95	63.75	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103488086 PE=3 ...	[more]
A0A0A0LPJ4	2.5e-94	62.21	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G369190 PE...	[more]
A0A6J1K4G1	1.9e-89	61.69	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492196 P...	[more]

BLAST of Moc08g02330.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G72220.1	2.0e-40	40.68	RING/U-box superfamily protein	[more]
AT5G10380.1	3.1e-36	49.38	RING/U-box superfamily protein	[more]
AT4G33565.1	1.7e-31	50.81	RING/U-box superfamily protein	[more]
AT5G17600.1	1.8e-28	33.33	RING/U-box superfamily protein	[more]
AT3G03550.1	4.1e-25	31.17	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 189..230 e-value: 1.2E-6 score: 38.1
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 188..231 e-value: 3.9E-13 score: 49.5
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 189..231 score: 12.607302
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 164..236 e-value: 9.7E-21 score: 74.8
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 63..327
None	No IPR available	PANTHER	PTHR45676:SF121	TRANSCRIPTION FACTOR C2H2 FAMILY-RELATED	coord: 63..327
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 188..231 e-value: 5.49041E-18 score: 74.6457
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 183..249

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc08g02330	Moc08g02330	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc08g02330.1.exon1	Moc08g02330.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc08g02330.1	cds.Moc08g02330.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc08g02330.1	Moc08g02330.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane