Moc07g05850.1 (mRNA) Bitter gourd (OHB3-1) v2

BLAST of Moc07g05850.1 vs. NCBI nr
Match: XP_022137317.1 (uncharacterized protein LOC111008813 [Momordica charantia])

HSP 1 Score: 978.0 bits (2527), Expect = 5.0e-281
Identity = 500/528 (94.70%), Postives = 505/528 (95.64%), Query Frame = 0

Query: 191 QAESSHNPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEGSLNDGDLGESPFTLDVL 250
           +AESS NPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEG LNDGDLGESPFT DVL
Sbjct: 3   KAESSRNPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVL 62

Query: 251 EAPIPPKFKAPTVKPYDGLKDPKDYVEVFEGLMDFQAASDAIKCRVFQIALTGSARLWYR 310
           EAPIPPKFKAPTVKPYDG KDPKDYVEVFE LMDFQAASDAIKCR F+IALTGSARLWYR
Sbjct: 63  EAPIPPKFKAPTVKPYDGSKDPKDYVEVFESLMDFQAASDAIKCRAFRIALTGSARLWYR 122

Query: 311 RLPARSISTYSQLRREFLVQFSSRHYDKNTATHLATIRQKEGETLREYVTRFQEEQLKVA 370
           RLPA SISTYSQLRREFL  FSSRHYDK TATHLATIRQKEGETLREYVTRFQEEQLKVA
Sbjct: 123 RLPAXSISTYSQLRREFLAXFSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKVA 182

Query: 371 HCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKI 430
           HCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKI
Sbjct: 183 HCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKI 242

Query: 431 GQGRSGKDIEKADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIE 490
           G+GRSGKDIE ADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIE
Sbjct: 243 GRGRSGKDIENADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIE 302

Query: 491 ESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDCWELKRQIEDPIQDGYFKKFV 550
           ESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSD WELKRQIE+ IQDGYFKKFV
Sbjct: 303 ESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDYWELKRQIENLIQDGYFKKFV 362

Query: 551 GKPRTSSAEKKEERKRSRTPHRRTDRPAVINTIFGGPSGGQFGHKRKELARAARREVCII 610
           GKPRTSSAEKKEERKRSRTP RRTDRPAVINTIFGGPSGGQ G KRKELARAARREVCII
Sbjct: 363 GKPRTSSAEKKEERKRSRTPPRRTDRPAVINTIFGGPSGGQSGRKRKELARAARREVCII 422

Query: 611 REQRPTCPITFDSTDLEEVHLPHNDALVIAPLIDHVVVRRVLIDGGASANILSLPTYLAL 670
           REQRPTCPITFD  DLEEVHLPHNDALVIAPLIDHVVV RVL+DGG SANILSLPTYLAL
Sbjct: 423 REQRPTCPITFDGADLEEVHLPHNDALVIAPLIDHVVVXRVLVDGGTSANILSLPTYLAL 482

Query: 671 GWTRSQLTKSPTPLVGFSGESVIPEGCIDLLVTLGQDQTQVTQMAEFV 719
           GWTRSQL KSPTPLVGFSGESVIPEG IDL VTLGQDQTQVTQMAEFV
Sbjct: 483 GWTRSQLKKSPTPLVGFSGESVIPEGFIDLPVTLGQDQTQVTQMAEFV 530

BLAST of Moc07g05850.1 vs. NCBI nr
Match: XP_022150760.1 (uncharacterized protein LOC111018823 [Momordica charantia])

HSP 1 Score: 974.5 bits (2518), Expect = 5.5e-280
Identity = 521/631 (82.57%), Postives = 534/631 (84.63%), Query Frame = 0

Query: 187 SSNQQAESSHNPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEGSLNDGDLGESPFT 246
           SSNQQAESSHNPATP GVITREEFDQLRG+L+AQVEALKAKCEQKEG LNDGDLGESPFT
Sbjct: 2   SSNQQAESSHNPATPDGVITREEFDQLRGKLNAQVEALKAKCEQKEGPLNDGDLGESPFT 61

Query: 247 LDVLEAPIPPKFKAPTVKPYDGLKDPKDYVEVFEGLMDFQAASDAIKCRVFQIALTGSAR 306
            DVLE        APTVK YDG KDPKDYVEVFEGLMDFQAASDAIKCR FQIALTGSAR
Sbjct: 62  SDVLE--------APTVKSYDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSAR 121

Query: 307 LWYRRLPARSISTYSQLRREFLVQFSSRHYDKNTATHLATIRQKEGETLREYVTRFQEEQ 366
           LW                                                     FQE+Q
Sbjct: 122 LW-----------------------------------------------------FQEDQ 181

Query: 367 LKVAHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRP 426
           LKVA  SDDSAMCYFLTGLADEALTVKLG+EAPATFAEVLQKAKKVIDGQELLRTKTGRP
Sbjct: 182 LKVAQSSDDSAMCYFLTGLADEALTVKLGKEAPATFAEVLQKAKKVIDGQELLRTKTGRP 241

Query: 427 ERKIGQGRSGKDIEKADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEIL 486
           ER I +GRSGKD EKAD KSKDKGSFSSGRAE+RRA NGPTRSRPYERFTPTTIPISEIL
Sbjct: 242 ERGIDRGRSGKD-EKADLKSKDKGSFSSGRAEFRRAVNGPTRSRPYERFTPTTIPISEIL 301

Query: 487 TNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDCWELKRQIEDPIQDGYF 546
           TNIEESGMEKLLKRPEKLRGAPERR+KDKYCRFHREH HNTSD WELKRQIED IQD YF
Sbjct: 302 TNIEESGMEKLLKRPEKLRGAPERRNKDKYCRFHREHDHNTSDRWELKRQIEDLIQDDYF 361

Query: 547 KKFVGKPRTSSAEKKEERKRSRTPHRRTDRPAVINTIFGGPSGGQFGHKRKELARAARRE 606
           KKFVGKPRTSSAEKKEERK SRTP RR DRPAVINTIFGGPSGGQ GHKRKELARAARRE
Sbjct: 362 KKFVGKPRTSSAEKKEERKLSRTPLRRIDRPAVINTIFGGPSGGQSGHKRKELARAARRE 421

Query: 607 VCIIREQRPTCPITFDSTDLEEVHLPHNDALVIAPLIDHVVVRRVLIDGGASANILSLPT 666
           VCIIREQRPTCPITFDS DLEEVHLPHNDALVIAPLIDHVVVRRVL+D G SANI+SL T
Sbjct: 422 VCIIREQRPTCPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDEGVSANIVSLLT 481

Query: 667 YLALGWTRSQLTKSPTPLVGFSGESVIPEGCIDLLVTLGQDQTQVTQMAEFVVIDGRSAY 726
           YLALGWTRSQL KS TPLVGFS ESVIPEGCIDL VTLG DQTQVTQMAEFVVIDGRSAY
Sbjct: 482 YLALGWTRSQLKKSTTPLVGFSRESVIPEGCIDLPVTLGHDQTQVTQMAEFVVIDGRSAY 541

Query: 727 NAIFGRSIIHSFRAIPSTLHQILKYPTPNGVGTIRGEQTASRECYASALKGSSVCALETL 786
           NAIFGR IIHSFRAIPSTLHQ+LKY TPNGVG +RGEQ ASRECYASALKGSSVCALETL
Sbjct: 542 NAIFGRPIIHSFRAIPSTLHQVLKYSTPNGVGMVRGEQIASRECYASALKGSSVCALETL 570

Query: 787 AGRDGTLEFEADLPRREFAAPTEELELVPLL 818
             RDGTLEF+A+LPRREFAAPTEELELVPLL
Sbjct: 602 VSRDGTLEFKANLPRREFAAPTEELELVPLL 570

BLAST of Moc07g05850.1 vs. NCBI nr
Match: XP_022152854.1 (uncharacterized protein LOC111020479 [Momordica charantia])

HSP 1 Score: 951.8 bits (2459), Expect = 3.8e-273
Identity = 525/791 (66.37%), Postives = 567/791 (71.68%), Query Frame = 0

Query: 1   MVQPANSTNTADRRTLAASDAHQREVGAAVVEGQGHDGLATEPLRRSARITAHVLPPAHP 60
           MVQPANSTNTADRR LAA+  HQREVGA VVEGQGH+ L TEPL RSARIT  VLPPAHP
Sbjct: 1   MVQPANSTNTADRRALAANHGHQREVGAEVVEGQGHEDLGTEPLCRSARITTPVLPPAHP 60

Query: 61  RTSKATRGRGGTSKKSARGPAPAPTSENFDALQREMEAMRTQMRSMEEMYNEMILAAGAG 120
           + SK                                                        
Sbjct: 61  KPSK-------------------------------------------------------- 120

Query: 121 SRSENRMTRIDIREQRGSHLGPVEEEHPGDNESEGHTHRRGDLREHLNRKRGSSLRKGQS 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSRSHRSSNQQAESSHNPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEGSLNDGDL 240
                      AESS+NP TP GVITREEFDQL+ + DAQVEALKA+CE+KE S +DGDL
Sbjct: 181 -----------AESSYNPITP-GVITREEFDQLKSKFDAQVEALKARCEKKESSFDDGDL 240

Query: 241 GESPFTLDVLEAPIPPKFKAPTVKPYDGLKDPKDYVEVFEGLMDFQAASDAIKCRVFQIA 300
           GE  F+ D+LEA IPPKFK PT+KPYDG KDPKDYVEVFE LMDFQAA+DAIKC  FQIA
Sbjct: 241 GELSFSSDILEALIPPKFKTPTMKPYDGSKDPKDYVEVFESLMDFQAATDAIKCCAFQIA 300

Query: 301 LTGSARLWYRRLPARSISTYSQLRREFLVQFSSRHYDKNTATHLATIRQKEGETLREYVT 360
           LTGSARLWYRRLPAR ISTYSQLR+EF+ QFSSRHYD+ T THLATIRQKEGETLREYVT
Sbjct: 301 LTGSARLWYRRLPARLISTYSQLRKEFISQFSSRHYDRKTPTHLATIRQKEGETLREYVT 360

Query: 361 RFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLR 420
           RF EEQLKVAHCSDDSAMCYFLTGLADE LTVKL EEAPATFAEVLQK KKVIDGQELLR
Sbjct: 361 RFPEEQLKVAHCSDDSAMCYFLTGLADETLTVKLREEAPATFAEVLQKTKKVIDGQELLR 420

Query: 421 TKTGRPERKIGQGRSGKDIEKADPKSKDKG-SFSSGRAEYRRAENGPTRSRPYERFTPTT 480
           TKTGRPE+ I QGR+GKD  KAD KS+DKG S SS R +YRR+ +   +SRPYE +TPTT
Sbjct: 421 TKTGRPEKNIDQGRAGKDKGKADSKSRDKGPSSSSSRVDYRRSNSSHNQSRPYEHYTPTT 480

Query: 481 IPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDCWELKRQIED 540
           IPI EILTNIEE+GMEKLLKRPEKLRG PE+R+ DKYCRFHR+HGHNTS+ WELKRQIED
Sbjct: 481 IPIFEILTNIEETGMEKLLKRPEKLRGDPEKRNTDKYCRFHRDHGHNTSNYWELKRQIED 540

Query: 541 PIQDGYFKKFVGKPRTSSAEKKEERKRSRTPHRRTDRPAVINTIFGGPSGGQFGHKRKEL 600
            IQDGYFKKFVGKPR++S EKKEERKR RTP RR DRPAVIN             K+KEL
Sbjct: 541 LIQDGYFKKFVGKPRSNSVEKKEERKRLRTPPRRDDRPAVIN-------------KKKEL 600

Query: 601 ARAARREVCIIREQRPTCPITFDSTDLEEVHLPHNDALVIAPLIDHVVVRRVLIDGGASA 660
           AR ARREVCIIREQRPT  I F+  DLE VHLPHNDALVIAPLID V+VRR+L+DGGASA
Sbjct: 601 AREARREVCIIREQRPTSSIAFNHADLEGVHLPHNDALVIAPLIDLVLVRRILVDGGASA 650

Query: 661 NILSLPTYLALGWTRSQLTKSPTPLVGFSGESVIPEGCIDLLVTLGQDQTQVTQMAEFVV 720
           NILSL TYLALGWTRSQL KSPTPLVGFSGES+  EGCIDL V++ QD TQVTQMAEFVV
Sbjct: 661 NILSLSTYLALGWTRSQLKKSPTPLVGFSGESISLEGCIDLPVSIRQDDTQVTQMAEFVV 650

Query: 721 IDGRSAYNAIFGRSIIHSFRAIPSTLHQILKYPTPNGVGTIRGEQTASRECYASALKGSS 780
           IDGRSAYNAIFGR IIHSFRA+PSTLHQ+LKY T NGVGT+RGE   SRECYAS  K SS
Sbjct: 721 IDGRSAYNAIFGRPIIHSFRAVPSTLHQVLKYSTLNGVGTVRGELKTSRECYASVPKRSS 650

Query: 781 VCALETLAGRD 791
           VCALE    RD
Sbjct: 781 VCALEEQTIRD 650

BLAST of Moc07g05850.1 vs. NCBI nr
Match: XP_022152110.1 (uncharacterized protein LOC111019899 [Momordica charantia])

HSP 1 Score: 793.5 bits (2048), Expect = 1.7e-225
Identity = 406/444 (91.44%), Postives = 416/444 (93.69%), Query Frame = 0

Query: 378 MCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKIGQGRSGK 437
           MCYFLTGLADEALTVKL EEAPATFAEVLQKAKKVIDGQELLRT       KIGQGRSGK
Sbjct: 1   MCYFLTGLADEALTVKLVEEAPATFAEVLQKAKKVIDGQELLRT-------KIGQGRSGK 60

Query: 438 DIEKADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIEESGMEKL 497
           D+E  DPKSKDKGSFS+GRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIEESGMEKL
Sbjct: 61  DMENTDPKSKDKGSFSNGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIEESGMEKL 120

Query: 498 LKRPEKLRGAPERRSKDKYCRFHREHGHNTSDCWELKRQIEDPIQDGYFKKFVGKPRTSS 557
           LKRPEKLRGAPERRSKDKYCRFHREHGHNTSD WELK QIED IQDGYFKKFVGKPRTSS
Sbjct: 121 LKRPEKLRGAPERRSKDKYCRFHREHGHNTSDYWELKSQIEDLIQDGYFKKFVGKPRTSS 180

Query: 558 AEKKEERKRSRTPHRRTDRPAVINTIFGGPSGGQFGHKRKELARAARREVCIIREQRPTC 617
           AEKKEERKRSRTP RRTDRPAVINTIFGGPSGGQ GHKRK+LARAARREVCIIREQRPTC
Sbjct: 181 AEKKEERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKKLARAARREVCIIREQRPTC 240

Query: 618 PITFDSTDLEEVHLPHNDALVIAPLIDHVVVRRVLIDGGASANILSLPTYLALGWTRSQL 677
           PITFD  DL EVHLPHNDALVIAPLIDHVVVRRVL+DGGASANILSLPTYLALGWTRSQL
Sbjct: 241 PITFDXADLXEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYLALGWTRSQL 300

Query: 678 TKSPTPLVGFSGESVIPEGCIDLLVTLGQDQTQVTQMAEFVVIDGRSAYNAIFGRSIIHS 737
            KSPTPLVGFSGESV+PEGCIDL VTLGQDQT+VTQMAEFVV+DGRSAYNAIFGR IIHS
Sbjct: 301 KKSPTPLVGFSGESVVPEGCIDLPVTLGQDQTRVTQMAEFVVVDGRSAYNAIFGRPIIHS 360

Query: 738 FRAIPSTLHQILKYPTPNGVGTIRGEQTASRECYASALKGSSVCALETLAGRDGTLEFEA 797
           FRAIPSTLHQ+LKY TPNGVGT+RGEQTASRECYAS LKG+SVCALETL  RDGTLEFEA
Sbjct: 361 FRAIPSTLHQVLKYSTPNGVGTVRGEQTASRECYASXLKGTSVCALETLTSRDGTLEFEA 420

Query: 798 DLPRREFAAPTEELELVPLLSPEK 822
           DLP REFAAP EELELVPLLS EK
Sbjct: 421 DLPXREFAAPXEELELVPLLSXEK 437

BLAST of Moc07g05850.1 vs. NCBI nr
Match: XP_022150613.1 (uncharacterized protein LOC111018708, partial [Momordica charantia])

HSP 1 Score: 776.5 bits (2004), Expect = 2.2e-220
Identity = 393/422 (93.13%), Postives = 401/422 (95.02%), Query Frame = 0

Query: 231 KEGSLNDGDLGESPFTLDVLEAPIPPKFKAPTVKPYDGLKDPKDYVEVFEGLMDFQAASD 290
           K+ SLNDGDLGES FT DVLEAPIPPKFKAPTVKPYDG KDPKDYVEVFEGLMDF AASD
Sbjct: 44  KDDSLNDGDLGESSFTSDVLEAPIPPKFKAPTVKPYDGSKDPKDYVEVFEGLMDFHAASD 103

Query: 291 AIKCRVFQIALTGSARLWYRRLPARSISTYSQLRREFLVQFSSRHYDKNTATHLATIRQK 350
           AIKCR FQIALTGSARLWYRRLPARSISTYSQLRREFL QFSSR Y K T THLATIRQK
Sbjct: 104 AIKCRAFQIALTGSARLWYRRLPARSISTYSQLRREFLAQFSSRQYGKKTETHLATIRQK 163

Query: 351 EGETLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAK 410
           EG TLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGE+AP TFAEVLQKAK
Sbjct: 164 EGGTLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEKAPTTFAEVLQKAK 223

Query: 411 KVIDGQELLRTKTGRPERKIGQGRSGKDIEKADPKSKDKGSFSSGRAEYRRAENGPTRSR 470
           KVIDGQELLRTKTGRP+RKIG+GRSGKD+E+ADPKSKDKGSFSSGRAEYRRAE+GPT+SR
Sbjct: 224 KVIDGQELLRTKTGRPDRKIGRGRSGKDVERADPKSKDKGSFSSGRAEYRRAESGPTKSR 283

Query: 471 PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDC 530
           PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDC
Sbjct: 284 PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDC 343

Query: 531 WELKRQIEDPIQDGYFKKFVGKPRTSSAEKKEERKRSRTPHRRTDRPAVINTIFGGPSGG 590
           WELKRQIED IQDGYFKKFVGKPRTSSAEKKEERKRSRTP RRTDRPAVINTIFGGPSGG
Sbjct: 344 WELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEERKRSRTPPRRTDRPAVINTIFGGPSGG 403

Query: 591 QFGHKRKELARAARREVCIIREQRPTCPITFDSTDLEEVHLPHNDALVIAPLIDHVVVRR 650
           Q GHKRKELARAARREVCIIREQ PTCPITFD  D EEVHLPHNDA VIAPLIDHVVVRR
Sbjct: 404 QSGHKRKELARAARREVCIIREQGPTCPITFDGADSEEVHLPHNDARVIAPLIDHVVVRR 463

Query: 651 VL 653
           VL
Sbjct: 464 VL 465

BLAST of Moc07g05850.1 vs. ExPASy TrEMBL
Match: A0A6J1C7X5 (uncharacterized protein LOC111008813 OS=Momordica charantia OX=3673 GN=LOC111008813 PE=4 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 2.4e-281
Identity = 500/528 (94.70%), Postives = 505/528 (95.64%), Query Frame = 0

Query: 191 QAESSHNPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEGSLNDGDLGESPFTLDVL 250
           +AESS NPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEG LNDGDLGESPFT DVL
Sbjct: 3   KAESSRNPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVL 62

Query: 251 EAPIPPKFKAPTVKPYDGLKDPKDYVEVFEGLMDFQAASDAIKCRVFQIALTGSARLWYR 310
           EAPIPPKFKAPTVKPYDG KDPKDYVEVFE LMDFQAASDAIKCR F+IALTGSARLWYR
Sbjct: 63  EAPIPPKFKAPTVKPYDGSKDPKDYVEVFESLMDFQAASDAIKCRAFRIALTGSARLWYR 122

Query: 311 RLPARSISTYSQLRREFLVQFSSRHYDKNTATHLATIRQKEGETLREYVTRFQEEQLKVA 370
           RLPA SISTYSQLRREFL  FSSRHYDK TATHLATIRQKEGETLREYVTRFQEEQLKVA
Sbjct: 123 RLPAXSISTYSQLRREFLAXFSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKVA 182

Query: 371 HCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKI 430
           HCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKI
Sbjct: 183 HCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKI 242

Query: 431 GQGRSGKDIEKADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIE 490
           G+GRSGKDIE ADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIE
Sbjct: 243 GRGRSGKDIENADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIE 302

Query: 491 ESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDCWELKRQIEDPIQDGYFKKFV 550
           ESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSD WELKRQIE+ IQDGYFKKFV
Sbjct: 303 ESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDYWELKRQIENLIQDGYFKKFV 362

Query: 551 GKPRTSSAEKKEERKRSRTPHRRTDRPAVINTIFGGPSGGQFGHKRKELARAARREVCII 610
           GKPRTSSAEKKEERKRSRTP RRTDRPAVINTIFGGPSGGQ G KRKELARAARREVCII
Sbjct: 363 GKPRTSSAEKKEERKRSRTPPRRTDRPAVINTIFGGPSGGQSGRKRKELARAARREVCII 422

Query: 611 REQRPTCPITFDSTDLEEVHLPHNDALVIAPLIDHVVVRRVLIDGGASANILSLPTYLAL 670
           REQRPTCPITFD  DLEEVHLPHNDALVIAPLIDHVVV RVL+DGG SANILSLPTYLAL
Sbjct: 423 REQRPTCPITFDGADLEEVHLPHNDALVIAPLIDHVVVXRVLVDGGTSANILSLPTYLAL 482

Query: 671 GWTRSQLTKSPTPLVGFSGESVIPEGCIDLLVTLGQDQTQVTQMAEFV 719
           GWTRSQL KSPTPLVGFSGESVIPEG IDL VTLGQDQTQVTQMAEFV
Sbjct: 483 GWTRSQLKKSPTPLVGFSGESVIPEGFIDLPVTLGQDQTQVTQMAEFV 530

BLAST of Moc07g05850.1 vs. ExPASy TrEMBL
Match: A0A6J1D9E1 (uncharacterized protein LOC111018823 OS=Momordica charantia OX=3673 GN=LOC111018823 PE=4 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 2.7e-280
Identity = 521/631 (82.57%), Postives = 534/631 (84.63%), Query Frame = 0

Query: 187 SSNQQAESSHNPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEGSLNDGDLGESPFT 246
           SSNQQAESSHNPATP GVITREEFDQLRG+L+AQVEALKAKCEQKEG LNDGDLGESPFT
Sbjct: 2   SSNQQAESSHNPATPDGVITREEFDQLRGKLNAQVEALKAKCEQKEGPLNDGDLGESPFT 61

Query: 247 LDVLEAPIPPKFKAPTVKPYDGLKDPKDYVEVFEGLMDFQAASDAIKCRVFQIALTGSAR 306
            DVLE        APTVK YDG KDPKDYVEVFEGLMDFQAASDAIKCR FQIALTGSAR
Sbjct: 62  SDVLE--------APTVKSYDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSAR 121

Query: 307 LWYRRLPARSISTYSQLRREFLVQFSSRHYDKNTATHLATIRQKEGETLREYVTRFQEEQ 366
           LW                                                     FQE+Q
Sbjct: 122 LW-----------------------------------------------------FQEDQ 181

Query: 367 LKVAHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRP 426
           LKVA  SDDSAMCYFLTGLADEALTVKLG+EAPATFAEVLQKAKKVIDGQELLRTKTGRP
Sbjct: 182 LKVAQSSDDSAMCYFLTGLADEALTVKLGKEAPATFAEVLQKAKKVIDGQELLRTKTGRP 241

Query: 427 ERKIGQGRSGKDIEKADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEIL 486
           ER I +GRSGKD EKAD KSKDKGSFSSGRAE+RRA NGPTRSRPYERFTPTTIPISEIL
Sbjct: 242 ERGIDRGRSGKD-EKADLKSKDKGSFSSGRAEFRRAVNGPTRSRPYERFTPTTIPISEIL 301

Query: 487 TNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDCWELKRQIEDPIQDGYF 546
           TNIEESGMEKLLKRPEKLRGAPERR+KDKYCRFHREH HNTSD WELKRQIED IQD YF
Sbjct: 302 TNIEESGMEKLLKRPEKLRGAPERRNKDKYCRFHREHDHNTSDRWELKRQIEDLIQDDYF 361

Query: 547 KKFVGKPRTSSAEKKEERKRSRTPHRRTDRPAVINTIFGGPSGGQFGHKRKELARAARRE 606
           KKFVGKPRTSSAEKKEERK SRTP RR DRPAVINTIFGGPSGGQ GHKRKELARAARRE
Sbjct: 362 KKFVGKPRTSSAEKKEERKLSRTPLRRIDRPAVINTIFGGPSGGQSGHKRKELARAARRE 421

Query: 607 VCIIREQRPTCPITFDSTDLEEVHLPHNDALVIAPLIDHVVVRRVLIDGGASANILSLPT 666
           VCIIREQRPTCPITFDS DLEEVHLPHNDALVIAPLIDHVVVRRVL+D G SANI+SL T
Sbjct: 422 VCIIREQRPTCPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDEGVSANIVSLLT 481

Query: 667 YLALGWTRSQLTKSPTPLVGFSGESVIPEGCIDLLVTLGQDQTQVTQMAEFVVIDGRSAY 726
           YLALGWTRSQL KS TPLVGFS ESVIPEGCIDL VTLG DQTQVTQMAEFVVIDGRSAY
Sbjct: 482 YLALGWTRSQLKKSTTPLVGFSRESVIPEGCIDLPVTLGHDQTQVTQMAEFVVIDGRSAY 541

Query: 727 NAIFGRSIIHSFRAIPSTLHQILKYPTPNGVGTIRGEQTASRECYASALKGSSVCALETL 786
           NAIFGR IIHSFRAIPSTLHQ+LKY TPNGVG +RGEQ ASRECYASALKGSSVCALETL
Sbjct: 542 NAIFGRPIIHSFRAIPSTLHQVLKYSTPNGVGMVRGEQIASRECYASALKGSSVCALETL 570

Query: 787 AGRDGTLEFEADLPRREFAAPTEELELVPLL 818
             RDGTLEF+A+LPRREFAAPTEELELVPLL
Sbjct: 602 VSRDGTLEFKANLPRREFAAPTEELELVPLL 570

BLAST of Moc07g05850.1 vs. ExPASy TrEMBL
Match: A0A6J1DHB3 (uncharacterized protein LOC111020479 OS=Momordica charantia OX=3673 GN=LOC111020479 PE=4 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 1.8e-273
Identity = 525/791 (66.37%), Postives = 567/791 (71.68%), Query Frame = 0

Query: 1   MVQPANSTNTADRRTLAASDAHQREVGAAVVEGQGHDGLATEPLRRSARITAHVLPPAHP 60
           MVQPANSTNTADRR LAA+  HQREVGA VVEGQGH+ L TEPL RSARIT  VLPPAHP
Sbjct: 1   MVQPANSTNTADRRALAANHGHQREVGAEVVEGQGHEDLGTEPLCRSARITTPVLPPAHP 60

Query: 61  RTSKATRGRGGTSKKSARGPAPAPTSENFDALQREMEAMRTQMRSMEEMYNEMILAAGAG 120
           + SK                                                        
Sbjct: 61  KPSK-------------------------------------------------------- 120

Query: 121 SRSENRMTRIDIREQRGSHLGPVEEEHPGDNESEGHTHRRGDLREHLNRKRGSSLRKGQS 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSRSHRSSNQQAESSHNPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEGSLNDGDL 240
                      AESS+NP TP GVITREEFDQL+ + DAQVEALKA+CE+KE S +DGDL
Sbjct: 181 -----------AESSYNPITP-GVITREEFDQLKSKFDAQVEALKARCEKKESSFDDGDL 240

Query: 241 GESPFTLDVLEAPIPPKFKAPTVKPYDGLKDPKDYVEVFEGLMDFQAASDAIKCRVFQIA 300
           GE  F+ D+LEA IPPKFK PT+KPYDG KDPKDYVEVFE LMDFQAA+DAIKC  FQIA
Sbjct: 241 GELSFSSDILEALIPPKFKTPTMKPYDGSKDPKDYVEVFESLMDFQAATDAIKCCAFQIA 300

Query: 301 LTGSARLWYRRLPARSISTYSQLRREFLVQFSSRHYDKNTATHLATIRQKEGETLREYVT 360
           LTGSARLWYRRLPAR ISTYSQLR+EF+ QFSSRHYD+ T THLATIRQKEGETLREYVT
Sbjct: 301 LTGSARLWYRRLPARLISTYSQLRKEFISQFSSRHYDRKTPTHLATIRQKEGETLREYVT 360

Query: 361 RFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLR 420
           RF EEQLKVAHCSDDSAMCYFLTGLADE LTVKL EEAPATFAEVLQK KKVIDGQELLR
Sbjct: 361 RFPEEQLKVAHCSDDSAMCYFLTGLADETLTVKLREEAPATFAEVLQKTKKVIDGQELLR 420

Query: 421 TKTGRPERKIGQGRSGKDIEKADPKSKDKG-SFSSGRAEYRRAENGPTRSRPYERFTPTT 480
           TKTGRPE+ I QGR+GKD  KAD KS+DKG S SS R +YRR+ +   +SRPYE +TPTT
Sbjct: 421 TKTGRPEKNIDQGRAGKDKGKADSKSRDKGPSSSSSRVDYRRSNSSHNQSRPYEHYTPTT 480

Query: 481 IPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDCWELKRQIED 540
           IPI EILTNIEE+GMEKLLKRPEKLRG PE+R+ DKYCRFHR+HGHNTS+ WELKRQIED
Sbjct: 481 IPIFEILTNIEETGMEKLLKRPEKLRGDPEKRNTDKYCRFHRDHGHNTSNYWELKRQIED 540

Query: 541 PIQDGYFKKFVGKPRTSSAEKKEERKRSRTPHRRTDRPAVINTIFGGPSGGQFGHKRKEL 600
            IQDGYFKKFVGKPR++S EKKEERKR RTP RR DRPAVIN             K+KEL
Sbjct: 541 LIQDGYFKKFVGKPRSNSVEKKEERKRLRTPPRRDDRPAVIN-------------KKKEL 600

Query: 601 ARAARREVCIIREQRPTCPITFDSTDLEEVHLPHNDALVIAPLIDHVVVRRVLIDGGASA 660
           AR ARREVCIIREQRPT  I F+  DLE VHLPHNDALVIAPLID V+VRR+L+DGGASA
Sbjct: 601 AREARREVCIIREQRPTSSIAFNHADLEGVHLPHNDALVIAPLIDLVLVRRILVDGGASA 650

Query: 661 NILSLPTYLALGWTRSQLTKSPTPLVGFSGESVIPEGCIDLLVTLGQDQTQVTQMAEFVV 720
           NILSL TYLALGWTRSQL KSPTPLVGFSGES+  EGCIDL V++ QD TQVTQMAEFVV
Sbjct: 661 NILSLSTYLALGWTRSQLKKSPTPLVGFSGESISLEGCIDLPVSIRQDDTQVTQMAEFVV 650

Query: 721 IDGRSAYNAIFGRSIIHSFRAIPSTLHQILKYPTPNGVGTIRGEQTASRECYASALKGSS 780
           IDGRSAYNAIFGR IIHSFRA+PSTLHQ+LKY T NGVGT+RGE   SRECYAS  K SS
Sbjct: 721 IDGRSAYNAIFGRPIIHSFRAVPSTLHQVLKYSTLNGVGTVRGELKTSRECYASVPKRSS 650

Query: 781 VCALETLAGRD 791
           VCALE    RD
Sbjct: 781 VCALEEQTIRD 650

BLAST of Moc07g05850.1 vs. ExPASy TrEMBL
Match: A0A6J1DD03 (uncharacterized protein LOC111019899 OS=Momordica charantia OX=3673 GN=LOC111019899 PE=4 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 8.4e-226
Identity = 406/444 (91.44%), Postives = 416/444 (93.69%), Query Frame = 0

Query: 378 MCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKIGQGRSGK 437
           MCYFLTGLADEALTVKL EEAPATFAEVLQKAKKVIDGQELLRT       KIGQGRSGK
Sbjct: 1   MCYFLTGLADEALTVKLVEEAPATFAEVLQKAKKVIDGQELLRT-------KIGQGRSGK 60

Query: 438 DIEKADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIEESGMEKL 497
           D+E  DPKSKDKGSFS+GRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIEESGMEKL
Sbjct: 61  DMENTDPKSKDKGSFSNGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIEESGMEKL 120

Query: 498 LKRPEKLRGAPERRSKDKYCRFHREHGHNTSDCWELKRQIEDPIQDGYFKKFVGKPRTSS 557
           LKRPEKLRGAPERRSKDKYCRFHREHGHNTSD WELK QIED IQDGYFKKFVGKPRTSS
Sbjct: 121 LKRPEKLRGAPERRSKDKYCRFHREHGHNTSDYWELKSQIEDLIQDGYFKKFVGKPRTSS 180

Query: 558 AEKKEERKRSRTPHRRTDRPAVINTIFGGPSGGQFGHKRKELARAARREVCIIREQRPTC 617
           AEKKEERKRSRTP RRTDRPAVINTIFGGPSGGQ GHKRK+LARAARREVCIIREQRPTC
Sbjct: 181 AEKKEERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKKLARAARREVCIIREQRPTC 240

Query: 618 PITFDSTDLEEVHLPHNDALVIAPLIDHVVVRRVLIDGGASANILSLPTYLALGWTRSQL 677
           PITFD  DL EVHLPHNDALVIAPLIDHVVVRRVL+DGGASANILSLPTYLALGWTRSQL
Sbjct: 241 PITFDXADLXEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYLALGWTRSQL 300

Query: 678 TKSPTPLVGFSGESVIPEGCIDLLVTLGQDQTQVTQMAEFVVIDGRSAYNAIFGRSIIHS 737
            KSPTPLVGFSGESV+PEGCIDL VTLGQDQT+VTQMAEFVV+DGRSAYNAIFGR IIHS
Sbjct: 301 KKSPTPLVGFSGESVVPEGCIDLPVTLGQDQTRVTQMAEFVVVDGRSAYNAIFGRPIIHS 360

Query: 738 FRAIPSTLHQILKYPTPNGVGTIRGEQTASRECYASALKGSSVCALETLAGRDGTLEFEA 797
           FRAIPSTLHQ+LKY TPNGVGT+RGEQTASRECYAS LKG+SVCALETL  RDGTLEFEA
Sbjct: 361 FRAIPSTLHQVLKYSTPNGVGTVRGEQTASRECYASXLKGTSVCALETLTSRDGTLEFEA 420

Query: 798 DLPRREFAAPTEELELVPLLSPEK 822
           DLP REFAAP EELELVPLLS EK
Sbjct: 421 DLPXREFAAPXEELELVPLLSXEK 437

BLAST of Moc07g05850.1 vs. ExPASy TrEMBL
Match: A0A6J1D9W7 (uncharacterized protein LOC111018708 OS=Momordica charantia OX=3673 GN=LOC111018708 PE=4 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 1.1e-220
Identity = 393/422 (93.13%), Postives = 401/422 (95.02%), Query Frame = 0

Query: 231 KEGSLNDGDLGESPFTLDVLEAPIPPKFKAPTVKPYDGLKDPKDYVEVFEGLMDFQAASD 290
           K+ SLNDGDLGES FT DVLEAPIPPKFKAPTVKPYDG KDPKDYVEVFEGLMDF AASD
Sbjct: 44  KDDSLNDGDLGESSFTSDVLEAPIPPKFKAPTVKPYDGSKDPKDYVEVFEGLMDFHAASD 103

Query: 291 AIKCRVFQIALTGSARLWYRRLPARSISTYSQLRREFLVQFSSRHYDKNTATHLATIRQK 350
           AIKCR FQIALTGSARLWYRRLPARSISTYSQLRREFL QFSSR Y K T THLATIRQK
Sbjct: 104 AIKCRAFQIALTGSARLWYRRLPARSISTYSQLRREFLAQFSSRQYGKKTETHLATIRQK 163

Query: 351 EGETLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAK 410
           EG TLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGE+AP TFAEVLQKAK
Sbjct: 164 EGGTLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEKAPTTFAEVLQKAK 223

Query: 411 KVIDGQELLRTKTGRPERKIGQGRSGKDIEKADPKSKDKGSFSSGRAEYRRAENGPTRSR 470
           KVIDGQELLRTKTGRP+RKIG+GRSGKD+E+ADPKSKDKGSFSSGRAEYRRAE+GPT+SR
Sbjct: 224 KVIDGQELLRTKTGRPDRKIGRGRSGKDVERADPKSKDKGSFSSGRAEYRRAESGPTKSR 283

Query: 471 PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDC 530
           PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDC
Sbjct: 284 PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDC 343

Query: 531 WELKRQIEDPIQDGYFKKFVGKPRTSSAEKKEERKRSRTPHRRTDRPAVINTIFGGPSGG 590
           WELKRQIED IQDGYFKKFVGKPRTSSAEKKEERKRSRTP RRTDRPAVINTIFGGPSGG
Sbjct: 344 WELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEERKRSRTPPRRTDRPAVINTIFGGPSGG 403

Query: 591 QFGHKRKELARAARREVCIIREQRPTCPITFDSTDLEEVHLPHNDALVIAPLIDHVVVRR 650
           Q GHKRKELARAARREVCIIREQ PTCPITFD  D EEVHLPHNDA VIAPLIDHVVVRR
Sbjct: 404 QSGHKRKELARAARREVCIIREQGPTCPITFDGADSEEVHLPHNDARVIAPLIDHVVVRR 463

Query: 651 VL 653
           VL
Sbjct: 464 VL 465