Moc07g03610.1 (mRNA) Bitter gourd (OHB3-1) v2

BLAST of Moc07g03610.1 vs. NCBI nr
Match: KAG5574299.1 (hypothetical protein H5410_054433 [Solanum commersonii])

HSP 1 Score: 117.5 bits (293), Expect = 1.3e-22
Identity = 69/194 (35.57%), Postives = 109/194 (56.19%), Query Frame = 0

Query: 3   LTLWGDLAENEGLEVEENVKNNPTIIATNVKIEIYEGEVSLSTTLTSIILIDPNFSDAED 62
           +TLWGD AEN+G  +E+   + P +   +V++ I++G   +ST   S +LI+P F  A D
Sbjct: 155 VTLWGDFAENDGSFLEKLQDDKPILALCDVRVSIFKGRFGISTIPVSSVLINPMFQKAND 214

Query: 63  LDARREKLLDYTKMANINPSRRMQEAKVVSIQNLIEGI----DNVTYCSLIACIINVSNK 122
           L A RE +    K   I P++ M+ A  V +  ++ G     ++  Y    A I ++ NK
Sbjct: 215 LRAWREIIKADNKDLTITPTKVMKRAIEVPLAKILAGFSADSEDCMY-KFKATIKDILNK 274

Query: 123 ENPWYEKCKICCQRVYPNDSDVTAYCSNCNK-NVTFIRKYMLKLLVSDGEMDAHITAFEA 182
           + PWY  CK C ++V     + TA C+NCN  NV +  +Y L+L V +GE  A +  FEA
Sbjct: 275 DEPWYSACKKCHKKV--QFIEDTAACNNCNSDNVEYKMRYCLRLEVCNGERRARVILFEA 334

Query: 183 TKMLIGCDVSDMLQ 192
            K L+GC+V + ++
Sbjct: 335 AKYLLGCNVQEYIE 345

BLAST of Moc07g03610.1 vs. NCBI nr
Match: KAG5560633.1 (hypothetical protein RHGRI_003829 [Rhododendron griersonianum])

HSP 1 Score: 115.9 bits (289), Expect = 3.8e-22
Identity = 66/194 (34.02%), Postives = 104/194 (53.61%), Query Frame = 0

Query: 3   LTLWGDLAENEGLEVEENVKNNPTIIATNVKIEIYEGEVSLSTTLTSIILIDPNFSDAED 62
           LTLWGD+A NEG  +E+     P +  + VKI  Y G +S+ST   + + +DP+  + + 
Sbjct: 353 LTLWGDMATNEGDMLEKMKTKRPIVALSKVKISTYAGVLSMSTISITTLQVDPSIPETDA 412

Query: 63  LDARREKLLDYTKMANINPSRRMQEAKVVSIQNLIE----GIDNVTYCSLIACIINVSNK 122
           +  R    L  T     +  +  + A+ V I +++E       N TYCS  A II + N 
Sbjct: 413 VKKRFNHNLQKTP-GTTSSGKNKETAERVKISDVMERSLLEFQN-TYCSFKATIIRIDNM 472

Query: 123 ENPWYEKCKICCQRVYPNDSDVTAYCSNC-NKNVTFIRKYMLKLLVSDGEMDAHITAFEA 182
           E PWY  CK+C ++V P +  +   C+ C +KN     +Y++K+ V DGE   +IT FEA
Sbjct: 473 EQPWYNACKVCNKKVIPGEQGME--CTKCADKNSEHHPRYLMKITVGDGERQLYITLFEA 532

Query: 183 TKMLIGCDVSDMLQ 192
            + + GC V D ++
Sbjct: 533 AEKITGCSVVDFIK 542

BLAST of Moc07g03610.1 vs. NCBI nr
Match: KAG5563556.1 (hypothetical protein RHGRI_006105 [Rhododendron griersonianum])

HSP 1 Score: 115.2 bits (287), Expect = 6.5e-22
Identity = 69/195 (35.38%), Postives = 106/195 (54.36%), Query Frame = 0

Query: 3   LTLWGDLAENEGLEVEENVKNNPTIIATNVKIEIYEGEVSLSTTLTSIILIDPNFSDAED 62
           LTLWGD+A NEG  +E+     P +  + VKI  Y G +S+ST   + + +DP+  + + 
Sbjct: 226 LTLWGDMATNEGDMLEKMKTKRPIVALSKVKISTYAGVLSMSTISITTLQVDPSIPETDA 285

Query: 63  LDARREKLLDYTKMANINPSRRMQE-AKVVSIQNLIE----GIDNVTYCSLIACIINVSN 122
           +  R    L   KM     S + +E A+ V I +++E       N TYCS  A II + N
Sbjct: 286 VKKRFNHNLQ--KMPGTTSSGKNKETAERVKISDVMERSLLEFQN-TYCSFKATIIRIDN 345

Query: 123 KENPWYEKCKICCQRVYPNDSDVTAYCSNC-NKNVTFIRKYMLKLLVSDGEMDAHITAFE 182
            E PWY  CK+C ++V P +  +   C+ C +KN     +Y++K+ V DGE   +IT FE
Sbjct: 346 MEQPWYNACKVCNKKVIPGEQGME--CTKCADKNSEHHPRYLMKITVGDGERQLYITLFE 405

Query: 183 ATKMLIGCDVSDMLQ 192
           A + + GC V D ++
Sbjct: 406 AAEKITGCSVVDFIK 415

BLAST of Moc07g03610.1 vs. NCBI nr
Match: KAG5563555.1 (hypothetical protein RHGRI_006105 [Rhododendron griersonianum])

HSP 1 Score: 115.2 bits (287), Expect = 6.5e-22
Identity = 69/195 (35.38%), Postives = 106/195 (54.36%), Query Frame = 0

Query: 3    LTLWGDLAENEGLEVEENVKNNPTIIATNVKIEIYEGEVSLSTTLTSIILIDPNFSDAED 62
            LTLWGD+A NEG  +E+     P +  + VKI  Y G +S+ST   + + +DP+  + + 
Sbjct: 1667 LTLWGDMATNEGDMLEKMKTKRPIVALSKVKISTYAGVLSMSTISITTLQVDPSIPETDA 1726

Query: 63   LDARREKLLDYTKMANINPSRRMQE-AKVVSIQNLIE----GIDNVTYCSLIACIINVSN 122
            +  R    L   KM     S + +E A+ V I +++E       N TYCS  A II + N
Sbjct: 1727 VKKRFNHNLQ--KMPGTTSSGKNKETAERVKISDVMERSLLEFQN-TYCSFKATIIRIDN 1786

Query: 123  KENPWYEKCKICCQRVYPNDSDVTAYCSNC-NKNVTFIRKYMLKLLVSDGEMDAHITAFE 182
             E PWY  CK+C ++V P +  +   C+ C +KN     +Y++K+ V DGE   +IT FE
Sbjct: 1787 MEQPWYNACKVCNKKVIPGEQGME--CTKCADKNSEHHPRYLMKITVGDGERQLYITLFE 1846

Query: 183  ATKMLIGCDVSDMLQ 192
            A + + GC V D ++
Sbjct: 1847 AAEKITGCSVVDFIK 1856

BLAST of Moc07g03610.1 vs. NCBI nr
Match: KAG5586050.1 (hypothetical protein H5410_046484 [Solanum commersonii])

HSP 1 Score: 114.8 bits (286), Expect = 8.5e-22
Identity = 67/195 (34.36%), Postives = 111/195 (56.92%), Query Frame = 0

Query: 3   LTLWGDLAENEGLEVEENVKNNPTIIATNVKIEIYEGEVSLSTTLTSIILIDPNFSDAED 62
           +TL GD AEN+G  +E+   + P +   +V++ IY+G+  +ST     +LI+P F    D
Sbjct: 129 VTLLGDFAENDGAFLEKLQDDKPLLALCDVRVSIYKGQFGISTIPVRSVLINPMFQKVND 188

Query: 63  LDARREKLLDYTKMANINPSRRMQEAKVVSIQNLIEGI----DNVTYCSLIACIINVSNK 122
           L A  E +    K   I P++ M+ A  V++ N+++G+    ++  Y    A I ++ NK
Sbjct: 189 LRAWSEIIEANNKDITITPTKVMRRAIEVTLANILDGLFADSEDCMY-KFKATIEDILNK 248

Query: 123 ENPWYEKCKICCQRV-YPNDSDVTAYCSNCN-KNVTFIRKYMLKLLVSDGEMDAHITAFE 182
           + PWY  CK C ++V +  D   TA C+NCN +NV +  +Y L+L V DGE  A +  FE
Sbjct: 249 DEPWYSSCKKCHKKVRFIKD---TAACNNCNSQNVEYEMRYCLRLEVCDGERHARVILFE 308

Query: 183 ATKMLIGCDVSDMLQ 192
           A K ++GC + + ++
Sbjct: 309 AAKYILGCSMQEYIE 319

BLAST of Moc07g03610.1 vs. ExPASy Swiss-Prot
Match: Q10Q08 (Replication protein A 70 kDa DNA-binding subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=RPA1B PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 9.2e-11
Identity = 57/204 (27.94%), Postives = 99/204 (48.53%), Query Frame = 0

Query: 3   LTLWGDLAENEGLEVEENVKNNPTIIATNVKIEIYEGEVSLSTTLTSIILIDPNFSDAED 62
           ++LW DLA   G E+ + V + P I   ++K+  ++G +SLST   S I+++P+  +AE 
Sbjct: 362 ISLWNDLATTTGQELLDMVDSAPIIAIKSLKVSDFQG-LSLSTVGRSTIVVNPDLPEAEQ 421

Query: 63  LDARREKLLDYTKMANINP--------------SRRMQEAKVVSIQNLIEGIDNVTYCSL 122
           L A  +     T MA+I                S R+  + + S  NL  G D   + SL
Sbjct: 422 LRAWYDSEGKGTSMASIGSDMGASRVGGARSMYSDRVFLSHITSDPNL--GQDKPVFFSL 481

Query: 123 IACIINVSNKENPWYEKCKICCQRVYPNDSDVTAY-CSNCNKN-VTFIRKYMLKLLVSDG 182
            A I  +   +  WY  CK C ++V   ++  + Y C  C KN      +Y++ + VSD 
Sbjct: 482 NAYISLIKPDQTMWYRACKTCNKKV--TEAIGSGYWCEGCQKNDAECSLRYIMVIKVSDP 541

Query: 183 EMDAHITAF-EATKMLIGCDVSDM 190
             +A ++ F +  + ++GC   ++
Sbjct: 542 TGEAWLSLFNDQAERIVGCSADEL 560

BLAST of Moc07g03610.1 vs. ExPASy Swiss-Prot
Match: Q9SD82 (Replication protein A 70 kDa DNA-binding subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA1B PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.6e-10
Identity = 56/201 (27.86%), Postives = 94/201 (46.77%), Query Frame = 0

Query: 3   LTLWGDLAENEGLEVEENVKNNPTIIATNVKIEIYEGEVSLSTTLTSIILIDPNFSDAED 62
           ++LW DLA   G E+ +   N+P I   ++K+  ++G VSLST   S ++I+PN  +A  
Sbjct: 334 VSLWNDLATGIGQELLDMADNHPVIAIKSLKVGAFQG-VSLSTISRSNVVINPNSPEATK 393

Query: 63  LDARREKLLDYTKMANI---------NPSRRMQEAKVV---SIQNLIEGIDNVTYCSLIA 122
           L +  +     T M+ I         N SR M   +V       N   G +   + S  A
Sbjct: 394 LKSWYDAEGKETSMSAIGSGMSSSANNGSRSMYSDRVFLSHITSNPSLGEEKPVFFSTRA 453

Query: 123 CIINVSNKENPWYEKCKICCQRVYPNDSDVTAYCSNC-NKNVTFIRKYMLKLLVSDGEMD 182
            I  +   +  WY  CK C ++V     D   +C +C  K+     +Y++ + VSD   +
Sbjct: 454 YISFIKPDQTMWYRACKTCNKKV-TEAMDSGYWCESCQKKDQECSLRYIMAVKVSDSTGE 513

Query: 183 AHITAF-EATKMLIGCDVSDM 190
             ++AF +  + +IGC   D+
Sbjct: 514 TWLSAFNDEAEKIIGCTADDL 532

BLAST of Moc07g03610.1 vs. ExPASy TrEMBL
Match: M1DP79 (Replication factor A OS=Solanum tuberosum OX=4113 PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 3.4e-24
Identity = 72/193 (37.31%), Postives = 110/193 (56.99%), Query Frame = 0

Query: 3   LTLWGDLAENEGLEVEENVKNNPTIIATNVKIEIYEGEVSLSTTLTSIILIDPNFSDAED 62
           +TLWGD AEN+G  +E+   + P +   +V++ IY+G   +ST   S +LI+P F  A D
Sbjct: 155 VTLWGDFAENDGAFLEKLKDDQPILGLCDVRVSIYKGTFGISTIPVSSVLINPIFQKALD 214

Query: 63  LDARREKLLDYTKMANINPSRRMQEAKVVSIQNLIEGIDNVTYCSLI---ACIINVSNKE 122
           L A RE +    K     PS+ M++A  V++ N+++G    T  +L    A I+++ N E
Sbjct: 215 LRAWREVIKAEKKPITTTPSKVMRKATEVTLLNIMDGTLADTQEALYKFKAKIVDILNIE 274

Query: 123 NPWYEKCKICCQRVYPNDSDVTAYCSNCN-KNVTFIRKYMLKLLVSDGEMDAHITAFEAT 182
            PWY  CK C ++V      VT  C NCN +N  +  +Y L+L V D E  A +T F+A 
Sbjct: 275 EPWYSSCKQCHKKVQVKQDTVT--CPNCNIQNGEYEMRYSLRLEVCDEEQRARVTLFDAA 334

Query: 183 KMLIGCDVSDMLQ 192
           +ML+GC V D ++
Sbjct: 335 QMLLGCTVEDYIK 345

BLAST of Moc07g03610.1 vs. ExPASy TrEMBL
Match: A0A1S4BAI4 (replication protein A 70 kDa DNA-binding subunit B-like OS=Nicotiana tabacum OX=4097 GN=LOC107806315 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 4.6e-21
Identity = 69/199 (34.67%), Postives = 113/199 (56.78%), Query Frame = 0

Query: 3   LTLWGDLAENEGLEVEENVKNNPTIIATNVKIEIYEGEVSLSTTLTSIILIDPNFSDAED 62
           +TLW D +E +G  +++ +   P + A +V+   Y G  S+STT  S I+I+P F  A  
Sbjct: 180 VTLWNDFSEIDGQVLQKLIDEKPVVAACDVRKTSYRGNFSISTTYVSSIMINPKFEKAVA 239

Query: 63  LDARREKLLDYTKMANIN----PSRRMQEAKVVSIQNL-IEGIDNV--TYCSLIACIINV 122
           L    +K  DY K  NI+    PS +++ A+ V I+++  +  DN   TY    A I ++
Sbjct: 240 L----QKWHDYKKAKNIDISLLPSIQLKNAREVKIEDIKDDSFDNKEDTYFKFNARIKDI 299

Query: 123 SNKENPWYEKCKICCQRVYPNDSDVTAYCSNCN-KNVTFIRKYMLKLLVSDGEMDAHITA 182
            NK+ PWY  CK C ++V  +  +  A C +C  +NV +  +Y+LKL V D +   ++T 
Sbjct: 300 MNKDEPWYSSCKKCYKKV--DVINNIAKCHHCGVENVDYQERYILKLEVFDKKERCYVTL 359

Query: 183 FEATKMLIGCDVSDMLQKL 194
           FE+T+ L+GCDV+  +Q +
Sbjct: 360 FESTRYLLGCDVTTYIQSI 372

BLAST of Moc07g03610.1 vs. ExPASy TrEMBL
Match: A0A1J6KWZ0 (Rep_fac-A_C domain-containing protein OS=Nicotiana attenuata OX=49451 GN=A4A49_29609 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 4.6e-21
Identity = 65/195 (33.33%), Postives = 104/195 (53.33%), Query Frame = 0

Query: 3   LTLWGDLAENEGLEVEENVKNNPTIIATNVKIEIYEGEVSLSTTLTSIILIDPNFSDAED 62
           +TLWG++AE +G  ++  +   P ++  NVK   Y G   +STT  S ++I+P+F  A  
Sbjct: 141 VTLWGEIAEKDGDMLQNMIFEKPIVVLCNVKGTTYRGNFVISTTHVSSVMINPSFEKAIA 200

Query: 63  LDARREKLLDYTKMANINPSRRMQEAKVVSIQNL--IEGIDNV--TYCSLIACIINVSNK 122
           L     ++    K   + PSR ++ A+ V I ++   +  DN   TY      I  + NK
Sbjct: 201 LQNWHNRMKLQDKDITLMPSRLIKNARKVKIGDITHADSFDNTKDTYRKFNTKIKYIINK 260

Query: 123 ENPWYEKCKICCQRVYPNDSDVTAYCSNCNKNVTFIRKYMLKLLVSDGEMDAHITAFEAT 182
           ++PWY  C  C ++V   D+  T  C  C  NV +  +Y+LKL + D +   +IT FEAT
Sbjct: 261 DDPWYLSCNKCYKKVSLVDNIAT--CPRCGSNVEYEERYLLKLELFDEKEHCYITLFEAT 320

Query: 183 KMLIGCDVSDMLQKL 194
           K L+GCDV   ++ +
Sbjct: 321 KYLLGCDVKTYVRSI 333

BLAST of Moc07g03610.1 vs. ExPASy TrEMBL
Match: M1DXI7 (Replication protein A1 OS=Solanum tuberosum OX=4113 PE=4 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.7e-20
Identity = 65/186 (34.95%), Postives = 103/186 (55.38%), Query Frame = 0

Query: 3   LTLWGDLAENEGLEVEENVKNNPTIIATNVKIEIYEGEVSLSTTLTSIILIDPNFSDAED 62
           +TLWGD AEN+G  +++   + P +   +V++ IY+G   +ST   S +LI+P F  A D
Sbjct: 180 VTLWGDFAENDGAFLDKLKDDKPILGLCDVRVSIYKGRFGISTIPVSSVLINPIFQKALD 239

Query: 63  LDARREKLLDYTKMANINPSRRMQEAKVVSIQNLIEGIDNVTYCSLI---ACIINVSNKE 122
           L A RE +    K   + P++ M+ A  V + +++ G+   +  S+    A I+++ NK+
Sbjct: 240 LRAWREIIEADNKDIIVAPTKAMKRAIEVPLDHILNGLLAESQDSMYKFKATIVDILNKD 299

Query: 123 NPWYEKCKICCQRVYPNDSDVTAYCSNCN-KNVTFIRKYMLKLLVSDGEMDAHITAFEAT 182
            PWY  CK C ++V        A C+NCN  NV +  +Y L+L V  GE  A +  FEA 
Sbjct: 300 EPWYLSCKTCRKKV--KVIKEAAACTNCNIDNVEYAMRYCLRLEVCGGEKRAQVVLFEAA 359

Query: 183 KMLIGC 185
             L+GC
Sbjct: 360 NNLLGC 363

BLAST of Moc07g03610.1 vs. ExPASy TrEMBL
Match: A0A314L4L3 (Replication protein a 70 kDa dna-binding subunit b OS=Nicotiana attenuata OX=49451 GN=RPA1B_0 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.1e-19
Identity = 69/191 (36.13%), Postives = 108/191 (56.54%), Query Frame = 0

Query: 3   LTLWGDLAENEGLEVEENVKNNPTIIA-TNVKIEIYEGEVSLSTTLTSIILIDPNFSDAE 62
           LTLWGDLAE EG E+ E+++N+  ++A  + K   ++G+  LSTT  S +LI P F  A+
Sbjct: 66  LTLWGDLAEIEG-ELLESIENSKPVVAFCDAKSSTFQGDFVLSTTPVSSVLIYPTFEKAK 125

Query: 63  DLDARREKLLDYTKMANINPSRRMQEAKVVSIQNLIEG-IDNV--TYCSLIACIINVSNK 122
           +L+   + +       ++ PSR MQ A+ V I ++I+G I N+   YC   A I ++++ 
Sbjct: 126 NLNKWNDNMKASKIDISLMPSRLMQIAREVKITDIIDGSIVNLKDIYCKFKAKITDINSN 185

Query: 123 ENPWYEKCKICCQRVYPNDSDVTAYCSNCN-KNVTFIRKYMLKLLVSDGEMDAHITAFEA 182
            +PWY  CK C +RV   +S  T  C  C  +++ +  +Y LK  VS  E    IT F+ 
Sbjct: 186 HDPWYHACKKCHKRVTVINSSAT--CGYCKIEDIDYDERYRLKFDVSAEEQYISITLFDG 245

Query: 183 TKMLIGCDVSD 189
            +   GCDV +
Sbjct: 246 ARYFFGCDVKE 253

BLAST of Moc07g03610.1 vs. TAIR 10
Match: AT5G08020.1 (RPA70-kDa subunit B )

HSP 1 Score: 66.2 bits (160), Expect = 3.2e-11
Identity = 56/201 (27.86%), Postives = 94/201 (46.77%), Query Frame = 0

Query: 3   LTLWGDLAENEGLEVEENVKNNPTIIATNVKIEIYEGEVSLSTTLTSIILIDPNFSDAED 62
           ++LW DLA   G E+ +   N+P I   ++K+  ++G VSLST   S ++I+PN  +A  
Sbjct: 334 VSLWNDLATGIGQELLDMADNHPVIAIKSLKVGAFQG-VSLSTISRSNVVINPNSPEATK 393

Query: 63  LDARREKLLDYTKMANI---------NPSRRMQEAKVV---SIQNLIEGIDNVTYCSLIA 122
           L +  +     T M+ I         N SR M   +V       N   G +   + S  A
Sbjct: 394 LKSWYDAEGKETSMSAIGSGMSSSANNGSRSMYSDRVFLSHITSNPSLGEEKPVFFSTRA 453

Query: 123 CIINVSNKENPWYEKCKICCQRVYPNDSDVTAYCSNC-NKNVTFIRKYMLKLLVSDGEMD 182
            I  +   +  WY  CK C ++V     D   +C +C  K+     +Y++ + VSD   +
Sbjct: 454 YISFIKPDQTMWYRACKTCNKKV-TEAMDSGYWCESCQKKDQECSLRYIMAVKVSDSTGE 513

Query: 183 AHITAF-EATKMLIGCDVSDM 190
             ++AF +  + +IGC   D+
Sbjct: 514 TWLSAFNDEAEKIIGCTADDL 532