Moc04g39620.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g39620.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	subtilisin-like protease SBT3.4
Location	chr4: 29402440 .. 29404188 (-)
Sequence length	381
RNA-Seq Expression	Moc04g39620.1
Synteny	Moc04g39620.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGAAGTACTGCCTTGCGATCTTCATTATTGCTTCTGTTGGTGTCTTTCTTCGCCATTTCGATATCTGCTGCCATGGCTGAGGCTGATGAAACTTCTGCCACCGCTTCCACGGTCTCGTCCTCTGCGTCATCGCGGCCCGCAGTTCACATCGTCTACACCGAAACGCCAACGGATGAGGAACCCGAGCACTTCCACATCCGAACCCTAGCCTCTGTCCTCGGCAGGTTCGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCCATGCGAAGAGAAGATCAGGGAATCGAAATTGGAAAATAAGAGCGCCGCTGGTTTCCATTTGCAGTGAGGAGGCGGCGAAGGATGCGCTTATTTACAGCTACAAGTCGGCTGCTCGTGGATTCTCTGCAAAGCTCACTCCCGACCAGGTTTCTCGAATTTCCCGTAAGTTTAATATCACTTGTCTGTTTCTCGAAATCGATTTTGTTAATTGAATTGATTAAGTTTGATGGATCGAGGATGATATCTAGGGATTCATGTTCTCCACAACTCAAATGCATGAGTTGTGGACTGATCAGACGATGTCTTCAGCTTTGAATAGCTTGCATCTTCTGCATTAACCATTGTATCATGGCGTTTGGGGTTTTGGCTTCCTTAACCTAAACCCATGCCGAGCAGGAAATCTTTTTTTTCTTTTCAAATCTTCCTCTTGTCAATTCTACTGTTTTGTGTTTTTTGAAATCCTCTGAATTGACTAACTTCGTGAGATACGTGGTCTGAGCATCGACGATCAATGAGATTCCTATACTTCATGCTCTTGCTCTTTTGTGTGGCTTATGAACGACGAATCTTTTTTTTCTTTCGTTCATGCTTGTATGACTATTTTTTGTAGGCAGTTTTGGGCATTTCATTTGTGCCTAAATTATTATAACTAACAACTAATTTTCATGTACATGTGACACATTTATATCTACATGAATAATTAAATACAAAAACTAAAAGTCTTTTTTAAGCTTGGATTAGCCACGCTTTTAACCTATATTTTGCCTGTTTTCAATTAGCCACGCTTGAAGCTTGGATTGATTCTTTTACTTCTATCTCTTTACGCTCAAGATTCATCTTTTCTATTACCATTTTAATATTGCTTTGTTTTTTTCCTTAGATCCTCTTTCAAATTCTTCTTCTCAAAGTCTTTTTTAAGTGATAGCTATGAAATAGTTTAAGTTGAATTATACTTCAAAATTTAAACTTCATTAATTCTTTGCAATCATCGATGAAAGAGGATTTGACAATTAGTTTGTTCAAATAAAATGATCTTTTAAATCCTTTAACGCATTTAGCTAGCAAGTTATGGTGGTTACAAATGGCCAATATAATAAGGGTTACCCCCAACAAGTTATCATCGTCCGTTTAGTATTATTTTGTTAATAGACATAGAAATTTCTCCGTAATTTTGGTATTTATGTTCATAAACTTAGTAGTTGATTCAAATCCAGTTTCAAATGAACGAATACAAGATATTTATGAATTATCGGATCTGGTTTGTTAATTCAATGTATACTTCAATAATTGCCATGGAGATGGTGAAAAGTCCTATATCTGTCATTTTGTGTTCTGGCAATAATTGCCTCAATGTTGTTTTAGGTTGTTCACTCATTAGCTGACTGACCAAACAAATTTGTCTGCTTCGCTCGGCAGAACAACCAGGTGTTCTACAGGTTGTACCAGACAGCCAACTCGAGTTGCACCGTTAG

mRNA sequence

ATGCGAAGTACTGCCTTGCGATCTTCATTATTGCTTCTGTTGGTGTCTTTCTTCGCCATTTCGATATCTGCTGCCATGGCTGAGGCTGATGAAACTTCTGCCACCGCTTCCACGGTCTCGTCCTCTGCGTCATCGCGGCCCGCAGTTCACATCGTCTACACCGAAACGCCAACGGATGAGGAACCCGAGCACTTCCACATCCGAACCCTAGCCTCTGTCCTCGGCAGTGAGGAGGCGGCGAAGGATGCGCTTATTTACAGCTACAAGTCGGCTGCTCGTGGATTCTCTGCAAAGCTCACTCCCGACCAGGTTTCTCGAATTTCCCAACAACCAGGTGTTCTACAGGTTGTACCAGACAGCCAACTCGAGTTGCACCGTTAG

Coding sequence (CDS)

ATGCGAAGTACTGCCTTGCGATCTTCATTATTGCTTCTGTTGGTGTCTTTCTTCGCCATTTCGATATCTGCTGCCATGGCTGAGGCTGATGAAACTTCTGCCACCGCTTCCACGGTCTCGTCCTCTGCGTCATCGCGGCCCGCAGTTCACATCGTCTACACCGAAACGCCAACGGATGAGGAACCCGAGCACTTCCACATCCGAACCCTAGCCTCTGTCCTCGGCAGTGAGGAGGCGGCGAAGGATGCGCTTATTTACAGCTACAAGTCGGCTGCTCGTGGATTCTCTGCAAAGCTCACTCCCGACCAGGTTTCTCGAATTTCCCAACAACCAGGTGTTCTACAGGTTGTACCAGACAGCCAACTCGAGTTGCACCGTTAG

Protein sequence

MRSTALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDEEPEHFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDSQLELHR

Homology

[Close]

BLAST of Moc04g39620.1 vs. NCBI nr
Match: XP_022152506.1 (subtilisin-like protease SBT3.3 isoform X1 [Momordica charantia] >XP_022152507.1 subtilisin-like protease SBT3.3 isoform X1 [Momordica charantia])

HSP 1 Score: 224.6 bits (571), Expect = 4.9e-55
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 1   MRSTALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDE 60
           MRSTALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDE
Sbjct: 1   MRSTALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDE 60

Query: 61  EPEHFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDS 120
           EPEHFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDS
Sbjct: 61  EPEHFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDS 120

Query: 121 QLELHR 127
           QLELHR
Sbjct: 121 QLELHR 126

[Close]

BLAST of Moc04g39620.1 vs. NCBI nr
Match: XP_022152508.1 (subtilisin-like protease SBT3.3 isoform X2 [Momordica charantia])

HSP 1 Score: 189.9 bits (481), Expect = 1.3e-44
Identity = 108/109 (99.08%), Postives = 109/109 (100.00%), Query Frame = 0


Query: 1   MRSTALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDE 60
           MRSTALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDE
Sbjct: 1   MRSTALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDE 60

Query: 61  EPEHFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQ 110
           EPEHFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRIS+
Sbjct: 61  EPEHFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISR 109

[Close]

BLAST of Moc04g39620.1 vs. NCBI nr
Match: XP_023550028.1 (subtilisin-like protease SBT3.4 [Cucurbita pepo subsp. pepo] >XP_023550029.1 subtilisin-like protease SBT3.4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 177.2 bits (448), Expect = 9.0e-41
Identity = 95/118 (80.51%), Postives = 106/118 (89.83%), Query Frame = 0


Query: 9   SLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDEEPEHFHIR 68
           +LL L VSFF ISIS+AMAE DETS+T S  SS+ASSRPAVHIVYTE+P DE+P+ FHIR
Sbjct: 14  ALLFLFVSFFTISISSAMAETDETSSTVSIASSAASSRPAVHIVYTESPRDEDPKDFHIR 73

Query: 69  TLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDSQLELHR 127
           TLAS LGSEEAAKDALIYSYK+A  GFSAKLTPDQVSR+SQQPGVLQV+PDSQ+ELHR
Sbjct: 74  TLASALGSEEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQVIPDSQVELHR 131

[Close]

BLAST of Moc04g39620.1 vs. NCBI nr
Match: KAG6578330.1 (Subtilisin-like protease 3.6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 174.1 bits (440), Expect = 7.7e-40
Identity = 94/118 (79.66%), Postives = 106/118 (89.83%), Query Frame = 0


Query: 9   SLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDEEPEHFHIR 68
           +LL L+VSFF ISIS+AMAE DETS+T S  SS+ASSRPAVHIVYTE+P DE+P+ FHIR
Sbjct: 14  ALLFLVVSFFTISISSAMAETDETSSTVSIASSAASSRPAVHIVYTESPRDEDPKDFHIR 73

Query: 69  TLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDSQLELHR 127
           TLAS LGS+EAAKDALIYSYK+A  GFSAKLTPDQVSR+SQQPGVLQVVPDSQ+ LHR
Sbjct: 74  TLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQVVPDSQVGLHR 131

[Close]

BLAST of Moc04g39620.1 vs. NCBI nr
Match: KAG7015906.1 (Subtilisin-like protease SBT3.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 172.2 bits (435), Expect = 2.9e-39
Identity = 93/118 (78.81%), Postives = 105/118 (88.98%), Query Frame = 0


Query: 9   SLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDEEPEHFHIR 68
           +LL L+VSFF ISIS+AMAE DETS+T S  SS+ SSRPAVHIVYTE+P DE+P+ FHIR
Sbjct: 14  ALLFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTESPRDEDPKDFHIR 73

Query: 69  TLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDSQLELHR 127
           TLAS LGS+EAAKDALIYSYK+A  GFSAKLTPDQVSR+SQQPGVLQVVPDSQ+ LHR
Sbjct: 74  TLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQVVPDSQVGLHR 131

[Close]

BLAST of Moc04g39620.1 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 8.6e-10
Identity = 34/85 (40.00%), Postives = 54/85 (63.53%), Query Frame = 0


Query: 43  ASSRPAVHIVYTETPTDEEPE---HFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKL 102
           AS++  VHIVY      ++P+     H + L+S+LGS++ A ++++YSY+    GF+AKL
Sbjct: 23  ASAKSKVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKL 82

Query: 103 TPDQVSRISQQPGVLQVVPDSQLEL 125
           T  Q  +I+  P V+ V+PDS  EL
Sbjct: 83  TKSQAKKIADSPEVIHVIPDSYYEL 107

[Close]

BLAST of Moc04g39620.1 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.1e-09
Identity = 36/87 (41.38%), Postives = 54/87 (62.07%), Query Frame = 0


Query: 41  SSASSRPAVHIVYTETPTDEEPE---HFHIRTLASVLGSEEAAKDALIYSYKSAARGFSA 100
           + +S++  VHIVY      ++PE     H R L S+LGS+E A D+++YSY+    GF+A
Sbjct: 26  AESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAA 85

Query: 101 KLTPDQVSRISQQPGVLQVVPDSQLEL 125
           KLT  Q  +I+  P V+ V+PDS  +L
Sbjct: 86  KLTESQAKKIADLPDVVHVIPDSFYKL 112

[Close]

BLAST of Moc04g39620.1 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.6e-08
Identity = 44/123 (35.77%), Postives = 67/123 (54.47%), Query Frame = 0


Query: 5   ALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDEEPE- 64
           + RSS+LL+L+S   + ++A  A ++  S               VHIVY       +PE 
Sbjct: 3   SFRSSILLVLLSLITV-LNATRARSETES--------------KVHIVYLGEKKHHDPEF 62

Query: 65  --HFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDSQ 124
               H + LAS+LGS++ A D+++YSY+    GF+AKLT  Q  +I+  P V+ V+PD  
Sbjct: 63  VTESHHQMLASLLGSKKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGF 110

[Close]

BLAST of Moc04g39620.1 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.1e-08
Identity = 32/79 (40.51%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 49  VHIVYTETPTDEEPE---HFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVS 108
           V++VY      + PE     H + L S+LGS+EA  D+++YSY+    GF+AKLT  Q  
Sbjct: 29  VYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTESQAQ 88

Query: 109 RISQQPGVLQVVPDSQLEL 125
           +IS+ P V+QV+P++  E+
Sbjct: 89  QISELPEVVQVIPNTLYEM 107

[Close]

BLAST of Moc04g39620.1 vs. ExPASy Swiss-Prot
Match: Q9ZSB1 (Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 2.1e-08
Identity = 32/79 (40.51%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 49  VHIVYTETPTDEEPE---HFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVS 108
           V++VY      + PE     H + L S+LGS+EA  D+++YSY+    GF+AKLT  Q  
Sbjct: 29  VYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTESQAQ 88

Query: 109 RISQQPGVLQVVPDSQLEL 125
           +IS+ P V+QV+P++  E+
Sbjct: 89  QISELPEVVQVIPNTLYEM 107

[Close]

BLAST of Moc04g39620.1 vs. ExPASy TrEMBL
Match: A0A6J1DGF7 (subtilisin-like protease SBT3.3 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020217 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.4e-55
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 1   MRSTALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDE 60
           MRSTALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDE
Sbjct: 1   MRSTALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDE 60

Query: 61  EPEHFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDS 120
           EPEHFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDS
Sbjct: 61  EPEHFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDS 120

Query: 121 QLELHR 127
           QLELHR
Sbjct: 121 QLELHR 126

[Close]

BLAST of Moc04g39620.1 vs. ExPASy TrEMBL
Match: A0A6J1DF17 (subtilisin-like protease SBT3.3 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111020217 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 6.5e-45
Identity = 108/109 (99.08%), Postives = 109/109 (100.00%), Query Frame = 0


Query: 1   MRSTALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDE 60
           MRSTALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDE
Sbjct: 1   MRSTALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDE 60

Query: 61  EPEHFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQ 110
           EPEHFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRIS+
Sbjct: 61  EPEHFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISR 109

[Close]

BLAST of Moc04g39620.1 vs. ExPASy TrEMBL
Match: A0A6J1JST0 (subtilisin-like protease SBT3.4 OS=Cucurbita maxima OX=3661 GN=LOC111489448 PE=4 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.8e-39
Identity = 94/118 (79.66%), Postives = 104/118 (88.14%), Query Frame = 0


Query: 9   SLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDEEPEHFHIR 68
           +LL L VSFF ISIS+AMAE DETS+T S  SS+ASSR AVHIVYTE+P DE+P+ FHIR
Sbjct: 14  ALLFLFVSFFTISISSAMAETDETSSTVSIASSAASSRSAVHIVYTESPRDEDPKDFHIR 73

Query: 69  TLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDSQLELHR 127
           TLAS LGSEEAAKDALIYSYK+A  GFSAKLTPDQVSR+SQQPGVLQVVPDSQ+ LHR
Sbjct: 74  TLASALGSEEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQVVPDSQVGLHR 131

[Close]

BLAST of Moc04g39620.1 vs. ExPASy TrEMBL
Match: A0A1S3B2X4 (subtilisin-like protease SBT3.6 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103485421 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 6.5e-37
Identity = 89/119 (74.79%), Postives = 101/119 (84.87%), Query Frame = 0


Query: 8   SSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDEEPEHFHI 67
           + LL +LVSFFA+SIS AMA++ E S+T S  SS  SS PAVHIVYTETP DE P+ FHI
Sbjct: 7   NKLLFVLVSFFAVSISTAMADSVEASSTHSIASSMPSSPPAVHIVYTETPPDENPKDFHI 66

Query: 68  RTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDSQLELHR 127
           RTLAS LGSEEAAKDAL+YSYK+A  GFSAKLTPDQVSR+SQQPGVLQV+PDSQ+ LHR
Sbjct: 67  RTLASALGSEEAAKDALVYSYKTAVGGFSAKLTPDQVSRVSQQPGVLQVIPDSQVGLHR 125

[Close]

BLAST of Moc04g39620.1 vs. ExPASy TrEMBL
Match: A0A1S3B2Y7 (subtilisin-like protease SBT3.6 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485421 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 3.6e-35
Identity = 88/120 (73.33%), Postives = 101/120 (84.17%), Query Frame = 0


Query: 8   SSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDEEPEHFHI 67
           + LL +LVSFFA+SIS AMA++ E S+T S  SS  SS PAVHIVYTETP DE P+ FHI
Sbjct: 7   NKLLFVLVSFFAVSISTAMADSVEASSTHSIASSMPSSPPAVHIVYTETPPDENPKDFHI 66

Query: 68  RTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRIS-QQPGVLQVVPDSQLELHR 127
           RTLAS LGSEEAAKDAL+YSYK+A  GFSAKLTPDQVSR+S +QPGVLQV+PDSQ+ LHR
Sbjct: 67  RTLASALGSEEAAKDALVYSYKTAVGGFSAKLTPDQVSRVSPEQPGVLQVIPDSQVGLHR 126

[Close]

BLAST of Moc04g39620.1 vs. TAIR 10
Match: AT1G71950.1 (Proteinase inhibitor, propeptide )

HSP 1 Score: 119.8 bits (299), Expect = 1.6e-27
Identity = 67/116 (57.76%), Postives = 85/116 (73.28%), Query Frame = 0


Query: 11  LLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDEEPEHFHIRTL 70
           L+++V FF   +S+  A      A   T  ++ SS   VHI+YTE PTDEEP+ +H+RTL
Sbjct: 13  LIIIVFFFFFCLSSFFASI--VMADEYTGEATGSSEAKVHIIYTEKPTDEEPKTYHLRTL 72

Query: 71  ASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDSQLELHR 127
           +S LGSEEAAKDALIYSYK AA GFSAKLTP+QV+ IS+QPGV+QVVP    +LH+
Sbjct: 73  SSALGSEEAAKDALIYSYKEAASGFSAKLTPEQVAEISKQPGVIQVVPSQTYQLHK 126

[Close]

BLAST of Moc04g39620.1 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 64.7 bits (156), Expect = 6.1e-11
Identity = 34/85 (40.00%), Postives = 54/85 (63.53%), Query Frame = 0


Query: 43  ASSRPAVHIVYTETPTDEEPE---HFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKL 102
           AS++  VHIVY      ++P+     H + L+S+LGS++ A ++++YSY+    GF+AKL
Sbjct: 23  ASAKSKVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKL 82

Query: 103 TPDQVSRISQQPGVLQVVPDSQLEL 125
           T  Q  +I+  P V+ V+PDS  EL
Sbjct: 83  TKSQAKKIADSPEVIHVIPDSYYEL 107

[Close]

BLAST of Moc04g39620.1 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 64.3 bits (155), Expect = 8.0e-11
Identity = 36/87 (41.38%), Postives = 54/87 (62.07%), Query Frame = 0


Query: 41  SSASSRPAVHIVYTETPTDEEPE---HFHIRTLASVLGSEEAAKDALIYSYKSAARGFSA 100
           + +S++  VHIVY      ++PE     H R L S+LGS+E A D+++YSY+    GF+A
Sbjct: 26  AESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAA 85

Query: 101 KLTPDQVSRISQQPGVLQVVPDSQLEL 125
           KLT  Q  +I+  P V+ V+PDS  +L
Sbjct: 86  KLTESQAKKIADLPDVVHVIPDSFYKL 112

[Close]

BLAST of Moc04g39620.1 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-09
Identity = 44/123 (35.77%), Postives = 67/123 (54.47%), Query Frame = 0


Query: 5   ALRSSLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDEEPE- 64
           + RSS+LL+L+S   + ++A  A ++  S               VHIVY       +PE 
Sbjct: 3   SFRSSILLVLLSLITV-LNATRARSETES--------------KVHIVYLGEKKHHDPEF 62

Query: 65  --HFHIRTLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDSQ 124
               H + LAS+LGS++ A D+++YSY+    GF+AKLT  Q  +I+  P V+ V+PD  
Sbjct: 63  VTESHHQMLASLLGSKKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGF 110

[Close]

BLAST of Moc04g39620.1 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-09
Identity = 43/108 (39.81%), Postives = 63/108 (58.33%), Query Frame = 0


Query: 23  SAAMAEADET---SATASTVSSSASSRPAVHIVYTETPTDEEPE---HFHIRTLASVLGS 82
           S A+  + ET   +   S V+ S++ R  VHIVY      ++PE     H R L S+LGS
Sbjct: 22  SKALKSSKETIFLTKERSFVAESSAKR-KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGS 81

Query: 83  EEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDSQLEL 125
           +E A D+++YSY+    GF+AKLT  Q  +I+  P V+ V+PDS  +L
Sbjct: 82  KEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKL 128

The following BLAST results are available for this feature:

BLAST of Moc04g39620.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152506.1	4.9e-55	100.00	subtilisin-like protease SBT3.3 isoform X1 [Momordica charantia] >XP_022152507.1...	[more]
XP_022152508.1	1.3e-44	99.08	subtilisin-like protease SBT3.3 isoform X2 [Momordica charantia]	[more]
XP_023550028.1	9.0e-41	80.51	subtilisin-like protease SBT3.4 [Cucurbita pepo subsp. pepo] >XP_023550029.1 sub...	[more]
KAG6578330.1	7.7e-40	79.66	Subtilisin-like protease 3.6, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7015906.1	2.9e-39	78.81	Subtilisin-like protease SBT3.6, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]

BLAST of Moc04g39620.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4HPF1	8.6e-10	40.00	Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S...	[more]
Q8L7I2	1.1e-09	41.38	Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S...	[more]
Q9MAP5	1.6e-08	35.77	Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 S...	[more]
Q9ZSB0	2.1e-08	40.51	Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 S...	[more]
Q9ZSB1	2.1e-08	40.51	Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3...	[more]

BLAST of Moc04g39620.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGF7	2.4e-55	100.00	subtilisin-like protease SBT3.3 isoform X1 OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1DF17	6.5e-45	99.08	subtilisin-like protease SBT3.3 isoform X2 OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1JST0	1.8e-39	79.66	subtilisin-like protease SBT3.4 OS=Cucurbita maxima OX=3661 GN=LOC111489448 PE=4...	[more]
A0A1S3B2X4	6.5e-37	74.79	subtilisin-like protease SBT3.6 isoform X3 OS=Cucumis melo OX=3656 GN=LOC1034854...	[more]
A0A1S3B2Y7	3.6e-35	73.33	subtilisin-like protease SBT3.6 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034854...	[more]

BLAST of Moc04g39620.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71950.1	1.6e-27	57.76	Proteinase inhibitor, propeptide	[more]
AT1G32950.1	6.1e-11	40.00	Subtilase family protein	[more]
AT4G10550.1	8.0e-11	41.38	Subtilase family protein	[more]
AT1G32960.1	1.1e-09	35.77	Subtilase family protein	[more]
AT4G10550.3	1.1e-09	39.81	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 60..125 e-value: 1.9E-13 score: 50.9
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 31..125 e-value: 5.4E-23 score: 83.3
None	No IPR available	PANTHER	PTHR48222	PROTEINASE INHIBITOR, PROPEPTIDE	coord: 15..125

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc04g39620	Moc04g39620	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc04g39620.1.exon3	Moc04g39620.1.exon3	exon
Moc04g39620.1.exon2	Moc04g39620.1.exon2	exon
Moc04g39620.1.exon1	Moc04g39620.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc04g39620.1	cds.Moc04g39620.1_3	CDS
cds.Moc04g39620.1	cds.Moc04g39620.1_2	CDS
cds.Moc04g39620.1	cds.Moc04g39620.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc04g39620.1	Moc04g39620.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008233	peptidase activity