Moc04g37210.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g37210.1
TypemRNA
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRING/U-box superfamily protein
Locationchr4: 27887749 .. 27889035 (+)
Sequence length1287
RNA-Seq ExpressionMoc04g37210.1
SyntenyMoc04g37210.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTATTTCTCCATTCCTAAGCTTCCTCACCAACCTAACATGCCTCTGCCTGATGAAACTGACGCTGATTGTGATTCTCCGGTGCCCACTGTACCATCCCTCCTCCCCAACCCCATGGATTTCAACCCCTTCGCCGCCGTCTCATCCGTCGTCGCATCTGCCGACGATTCCCTCCCCTTCGACACCGATTGGTTCTCCGATTCCGACTCCGACTCCGAGCGTGATGTTAGTTGCTTCGTGACTGATCTTTTCGAGAATCGTCGTTTCACGGATCATTTGCACGCTCTTGGCGATCCTGATCGGGGTTCCGACCCATTTACGCCTTCTGCTAACGACTTGGACTTGGGCATTTCGGGATTCGAAGACAGCGGTGAAATTGAATCCAATTATATTGAGGAGCTCTGGTCGACCTTCGCGGTGGAGTCCGATACCCGGGTTTCCGAGACGGATGTGAGCAGCATGCAGGTCGCGGTTGCGGATGCAATTAATGGTGGCCTTCGGCTTGTTGATGTGGATTCGGACTCCGATTCTGATAACGGAATGCTTGGTGCGCTGCAACTCGTGACGGGGGATGATGACGAGAATGATGTTAATTGGGTTGAGAGTTCCAATCCTCTCTCGGATAACTTCAGCTTTTGCATCCAGGGCCAGAGGATTTCGTACGAGGATTTCGAATGGGAGGAGGTGGAGGAGCGGGTGGACGACATGGAAAATCCAAGCGTAGTGGTGGATGGGGCTGAGGAATTGTCTCTTGCATCGGGGTTCTCCAATGAAGACGAGTCGGGAGAAGAGGCAAGCCGGTGGGAAATCCTATTAGTAATGAACGACATTGGGAGGAATACGAACTGGGAACGGGATAATGATTCTGAGGCATACATAGCCGAGCAGGATGATTATATGTACGCAGCAGAATATGATACCCTTTTCGGACAATTCGTGGAAAATGAGAATTCTAAGGGTAGTCCTCCAGCAGCTAAGACTGCTGTGGAGAATCTTCCTCTGGTTGAGTTGAAAATGGAGAATGTGCTGACAGAGGAAGTCGTTGTTTGTGCTGTTTGTAAGGACAAATTTTCAATGGAGGAGAAGGTGAGGAAGCTTCCCTGTGGCCACTATTACCATGATGATTGCATTCTGCCCTGGCTGAATATCCGAAACACATGTCCAGTTTGTCGGTATGAGTTACCTACTGACGATCCAGATTATGAGCGTAGGAAGAGCCAAAGGGCGGGTGGTGGCCTGCAAATGGATTTTCAGGTCAGGTACAATTTTGAACATATAGCTTAA

mRNA sequence

ATGGCGTATTTCTCCATTCCTAAGCTTCCTCACCAACCTAACATGCCTCTGCCTGATGAAACTGACGCTGATTGTGATTCTCCGGTGCCCACTGTACCATCCCTCCTCCCCAACCCCATGGATTTCAACCCCTTCGCCGCCGTCTCATCCGTCGTCGCATCTGCCGACGATTCCCTCCCCTTCGACACCGATTGGTTCTCCGATTCCGACTCCGACTCCGAGCGTGATGTTAGTTGCTTCGTGACTGATCTTTTCGAGAATCGTCGTTTCACGGATCATTTGCACGCTCTTGGCGATCCTGATCGGGGTTCCGACCCATTTACGCCTTCTGCTAACGACTTGGACTTGGGCATTTCGGGATTCGAAGACAGCGGTGAAATTGAATCCAATTATATTGAGGAGCTCTGGTCGACCTTCGCGGTGGAGTCCGATACCCGGGTTTCCGAGACGGATGTGAGCAGCATGCAGGTCGCGGTTGCGGATGCAATTAATGGTGGCCTTCGGCTTGTTGATGTGGATTCGGACTCCGATTCTGATAACGGAATGCTTGGTGCGCTGCAACTCGTGACGGGGGATGATGACGAGAATGATGTTAATTGGGTTGAGAGTTCCAATCCTCTCTCGGATAACTTCAGCTTTTGCATCCAGGGCCAGAGGATTTCGTACGAGGATTTCGAATGGGAGGAGGTGGAGGAGCGGGTGGACGACATGGAAAATCCAAGCGTAGTGGTGGATGGGGCTGAGGAATTGTCTCTTGCATCGGGGTTCTCCAATGAAGACGAGTCGGGAGAAGAGGCAAGCCGGTGGGAAATCCTATTAGTAATGAACGACATTGGGAGGAATACGAACTGGGAACGGGATAATGATTCTGAGGCATACATAGCCGAGCAGGATGATTATATGTACGCAGCAGAATATGATACCCTTTTCGGACAATTCGTGGAAAATGAGAATTCTAAGGGTAGTCCTCCAGCAGCTAAGACTGCTGTGGAGAATCTTCCTCTGGTTGAGTTGAAAATGGAGAATGTGCTGACAGAGGAAGTCGTTGTTTGTGCTGTTTGTAAGGACAAATTTTCAATGGAGGAGAAGGTGAGGAAGCTTCCCTGTGGCCACTATTACCATGATGATTGCATTCTGCCCTGGCTGAATATCCGAAACACATGTCCAGTTTGTCGGTATGAGTTACCTACTGACGATCCAGATTATGAGCGTAGGAAGAGCCAAAGGGCGGGTGGTGGCCTGCAAATGGATTTTCAGGTCAGGTACAATTTTGAACATATAGCTTAA

Coding sequence (CDS)

ATGGCGTATTTCTCCATTCCTAAGCTTCCTCACCAACCTAACATGCCTCTGCCTGATGAAACTGACGCTGATTGTGATTCTCCGGTGCCCACTGTACCATCCCTCCTCCCCAACCCCATGGATTTCAACCCCTTCGCCGCCGTCTCATCCGTCGTCGCATCTGCCGACGATTCCCTCCCCTTCGACACCGATTGGTTCTCCGATTCCGACTCCGACTCCGAGCGTGATGTTAGTTGCTTCGTGACTGATCTTTTCGAGAATCGTCGTTTCACGGATCATTTGCACGCTCTTGGCGATCCTGATCGGGGTTCCGACCCATTTACGCCTTCTGCTAACGACTTGGACTTGGGCATTTCGGGATTCGAAGACAGCGGTGAAATTGAATCCAATTATATTGAGGAGCTCTGGTCGACCTTCGCGGTGGAGTCCGATACCCGGGTTTCCGAGACGGATGTGAGCAGCATGCAGGTCGCGGTTGCGGATGCAATTAATGGTGGCCTTCGGCTTGTTGATGTGGATTCGGACTCCGATTCTGATAACGGAATGCTTGGTGCGCTGCAACTCGTGACGGGGGATGATGACGAGAATGATGTTAATTGGGTTGAGAGTTCCAATCCTCTCTCGGATAACTTCAGCTTTTGCATCCAGGGCCAGAGGATTTCGTACGAGGATTTCGAATGGGAGGAGGTGGAGGAGCGGGTGGACGACATGGAAAATCCAAGCGTAGTGGTGGATGGGGCTGAGGAATTGTCTCTTGCATCGGGGTTCTCCAATGAAGACGAGTCGGGAGAAGAGGCAAGCCGGTGGGAAATCCTATTAGTAATGAACGACATTGGGAGGAATACGAACTGGGAACGGGATAATGATTCTGAGGCATACATAGCCGAGCAGGATGATTATATGTACGCAGCAGAATATGATACCCTTTTCGGACAATTCGTGGAAAATGAGAATTCTAAGGGTAGTCCTCCAGCAGCTAAGACTGCTGTGGAGAATCTTCCTCTGGTTGAGTTGAAAATGGAGAATGTGCTGACAGAGGAAGTCGTTGTTTGTGCTGTTTGTAAGGACAAATTTTCAATGGAGGAGAAGGTGAGGAAGCTTCCCTGTGGCCACTATTACCATGATGATTGCATTCTGCCCTGGCTGAATATCCGAAACACATGTCCAGTTTGTCGGTATGAGTTACCTACTGACGATCCAGATTATGAGCGTAGGAAGAGCCAAAGGGCGGGTGGTGGCCTGCAAATGGATTTTCAGGTCAGGTACAATTTTGAACATATAGCTTAA

Protein sequence

MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSLLPNPMDFNPFAAVSSVVASADDSLPFDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDRGSDPFTPSANDLDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDVDSDSDSDNGMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEERVDDMENPSVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEAYIAEQDDYMYAAEYDTLFGQFVENENSKGSPPAAKTAVENLPLVELKMENVLTEEVVVCAVCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQMDFQVRYNFEHIA
Homology
BLAST of Moc04g37210.1 vs. NCBI nr
Match: XP_022134467.1 (uncharacterized protein LOC111006706 [Momordica charantia])

HSP 1 Score: 866.7 bits (2238), Expect = 8.4e-248
Identity = 428/428 (100.00%), Postives = 428/428 (100.00%), Query Frame = 0

Query: 1   MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSLLPNPMDFNPFAAVSSVVASADDSLP 60
           MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSLLPNPMDFNPFAAVSSVVASADDSLP
Sbjct: 1   MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSLLPNPMDFNPFAAVSSVVASADDSLP 60

Query: 61  FDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDRGSDPFTPSANDLDLGISG 120
           FDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDRGSDPFTPSANDLDLGISG
Sbjct: 61  FDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDRGSDPFTPSANDLDLGISG 120

Query: 121 FEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDVDSDSDSDN 180
           FEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDVDSDSDSDN
Sbjct: 121 FEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDVDSDSDSDN 180

Query: 181 GMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEERVDDMENP 240
           GMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEERVDDMENP
Sbjct: 181 GMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEERVDDMENP 240

Query: 241 SVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEAYIAEQDDY 300
           SVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEAYIAEQDDY
Sbjct: 241 SVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEAYIAEQDDY 300

Query: 301 MYAAEYDTLFGQFVENENSKGSPPAAKTAVENLPLVELKMENVLTEEVVVCAVCKDKFSM 360
           MYAAEYDTLFGQFVENENSKGSPPAAKTAVENLPLVELKMENVLTEEVVVCAVCKDKFSM
Sbjct: 301 MYAAEYDTLFGQFVENENSKGSPPAAKTAVENLPLVELKMENVLTEEVVVCAVCKDKFSM 360

Query: 361 EEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQMDFQV 420
           EEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQMDFQV
Sbjct: 361 EEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQMDFQV 420

Query: 421 RYNFEHIA 429
           RYNFEHIA
Sbjct: 421 RYNFEHIA 428

BLAST of Moc04g37210.1 vs. NCBI nr
Match: XP_022992755.1 (uncharacterized protein LOC111488995 isoform X4 [Cucurbita maxima])

HSP 1 Score: 693.3 bits (1788), Expect = 1.3e-195
Identity = 351/437 (80.32%), Postives = 383/437 (87.64%), Query Frame = 0

Query: 1   MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSL----LPNPMDFNPFAAVSSVVASAD 60
           M + SIPKL  QPN+ LPD+T +D  SPVP+VPSL    LPN MDFNPFA +SS+V SAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDR----GSDPFTPSAN 120
           DS PFDTDWFSD+DS+SERDVSCFVTDLFENRRFTDHLHA  D DR    G DPFT SAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDV 180
             DLGISGFED+GEIES+YIEELWS FA+ESD+RVSE DVS   + VAD+   GLR+VDV
Sbjct: 121 --DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVS---MPVADSTTSGLRIVDV 180

Query: 181 DSDSDSDNGMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEE 240
           DSDS+SDNGM+GALQLVTGDD END NWVE+ NPLSD+FSFC QGQRI YEDFEWEEVEE
Sbjct: 181 DSDSESDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEE 240

Query: 241 RVDDMENPSVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEA 300
           RVD+ +N S VVDGAEELSLASGFSNE+ESGEEA RWEILLVMN+I RNTNWERDND+EA
Sbjct: 241 RVDERDNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNNIVRNTNWERDNDAEA 300

Query: 301 YIAEQDDYMYAAEYDTLFGQFVENENS-KGSPPAAKTAVENLPLVELKMENVLTEEVVVC 360
           YIA+QDDYMYA EYDTLFGQFVENEN+ KGSPPAAK++VENLPLVELK EN+LTEE+V C
Sbjct: 301 YIADQDDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVAC 360

Query: 361 AVCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG 420
           AVCKDKFSMEEKVRKLPC HYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG
Sbjct: 361 AVCKDKFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG 420

Query: 421 GGLQMDFQVRYNFEHIA 429
           GGLQ D +VRYNFE IA
Sbjct: 421 GGLQTDLRVRYNFELIA 432

BLAST of Moc04g37210.1 vs. NCBI nr
Match: XP_023549550.1 (E3 ubiquitin ligase BIG BROTHER-related-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 692.6 bits (1786), Expect = 2.2e-195
Identity = 350/437 (80.09%), Postives = 383/437 (87.64%), Query Frame = 0

Query: 1   MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSL----LPNPMDFNPFAAVSSVVASAD 60
           M + SIPKL  QPN+ LPD+T +D  SPVP+VPSL    LPN MDFNPFAA+SS+V SAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFAAISSIVTSAD 60

Query: 61  DSLPFDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDR----GSDPFTPSAN 120
           DS PFDTDWFSD+DS+SERDVSCFVTDLFENRRFTDHLHA  D DR    G DPFT SAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDV 180
             DLGISGFED+GEIES+YIEELWS FA+ESD+RVSE DV+   + VAD+   GLR+VDV
Sbjct: 121 --DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVN---MPVADSTTSGLRIVDV 180

Query: 181 DSDSDSDNGMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEE 240
           DSDS+SDNGM+GALQLVTGDD END NWVE+ NPLSD+FSFC QGQRI YEDFEWEEVEE
Sbjct: 181 DSDSESDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEE 240

Query: 241 RVDDMENPSVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEA 300
           RVD+ +N S VVDGAEELSLASGFSNE+ESGEEA RWEILLVMNDI RNTNWERDND+EA
Sbjct: 241 RVDERDNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIVRNTNWERDNDAEA 300

Query: 301 YIAEQDDYMYAAEYDTLFGQFVENENS-KGSPPAAKTAVENLPLVELKMENVLTEEVVVC 360
           YIA+QDDYMYA EYDTLFGQFVENEN+ KGSPPAAK++VENLPLVELK EN+L EE+V C
Sbjct: 301 YIADQDDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLAEELVAC 360

Query: 361 AVCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG 420
           AVCKDKFSMEEKVRKLPC HYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKS+RAG
Sbjct: 361 AVCKDKFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSRRAG 420

Query: 421 GGLQMDFQVRYNFEHIA 429
           GGLQ D +VRYNFE IA
Sbjct: 421 GGLQTDLRVRYNFEPIA 432

BLAST of Moc04g37210.1 vs. NCBI nr
Match: XP_022938801.1 (uncharacterized protein LOC111444905 isoform X3 [Cucurbita moschata])

HSP 1 Score: 692.6 bits (1786), Expect = 2.2e-195
Identity = 350/437 (80.09%), Postives = 383/437 (87.64%), Query Frame = 0

Query: 1   MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSL----LPNPMDFNPFAAVSSVVASAD 60
           M + SIPKL  QPN+ LPD+T +D  SPVP+VPSL    LPN MDFNPFA +SS+V SAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDR----GSDPFTPSAN 120
           DS PFDTDWFSD+DS+SERDVSCFVTDLFENRRFTDHLHA  D DR    G DPFT SAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDV 180
             DLGISGFED+GEIES+YIEELWS FA+ESD+RVSE DVS   + VAD+   GLR+VDV
Sbjct: 121 --DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVS---MPVADSTTSGLRIVDV 180

Query: 181 DSDSDSDNGMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEE 240
           DSDS+SDNGM+GALQLVTGDD END NWVE+ NPLSD+FSFC QGQRI YEDFEWEEVEE
Sbjct: 181 DSDSESDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEE 240

Query: 241 RVDDMENPSVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEA 300
           RVD+ +N S VVDGAEELSLASGFSNE+E+GEEA RWEILLVMNDI RNTNWERDND+EA
Sbjct: 241 RVDERDNLSAVVDGAEELSLASGFSNEEEAGEEAGRWEILLVMNDIVRNTNWERDNDAEA 300

Query: 301 YIAEQDDYMYAAEYDTLFGQFVENENS-KGSPPAAKTAVENLPLVELKMENVLTEEVVVC 360
           YIA+QDDYMYA EYDTLFGQFVENEN+ KGSPPAAK++VENLPLVELK EN+LTEE+V C
Sbjct: 301 YIADQDDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVAC 360

Query: 361 AVCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG 420
           AVCKDKFSMEEKVRKLPC HYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKS+RAG
Sbjct: 361 AVCKDKFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSRRAG 420

Query: 421 GGLQMDFQVRYNFEHIA 429
           GGLQ D +VRYNFE IA
Sbjct: 421 GGLQTDLRVRYNFELIA 432

BLAST of Moc04g37210.1 vs. NCBI nr
Match: KAG7016086.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 688.0 bits (1774), Expect = 5.4e-194
Identity = 349/437 (79.86%), Postives = 381/437 (87.19%), Query Frame = 0

Query: 1   MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSL----LPNPMDFNPFAAVSSVVASAD 60
           M + SIPKL  QPN+ LPD+T +D  SPV +VPSL    LPN MDFNPFA +SS+V SAD
Sbjct: 24  MDFLSIPKLNLQPNVALPDQTHSDSPSPVLSVPSLRHDDLPNTMDFNPFATISSIVTSAD 83

Query: 61  DSLPFDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDR----GSDPFTPSAN 120
           DS PFDTDWFSD+DS+SERDVSCFVTDLFENRRFTDHLHA  D DR    G DPFT SAN
Sbjct: 84  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 143

Query: 121 DLDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDV 180
             DLGISGFED+GEIES+YIEELWS FA+ESD+RVSE DVS   + VAD+   GLR+VDV
Sbjct: 144 --DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVS---MPVADSTTSGLRIVDV 203

Query: 181 DSDSDSDNGMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEE 240
           DSDS+SDNGM+GALQLVTGDD END NWVE+ NPLSD+FSFC QGQRI YEDFEWEEVEE
Sbjct: 204 DSDSESDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEE 263

Query: 241 RVDDMENPSVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEA 300
           RVD+ +N S VVDGAEELSLASGFSNE+ESGEEA RWEILLVMNDI RNTNWERDND+EA
Sbjct: 264 RVDERDNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIVRNTNWERDNDAEA 323

Query: 301 YIAEQDDYMYAAEYDTLFGQFVENENS-KGSPPAAKTAVENLPLVELKMENVLTEEVVVC 360
           YIA+QDDYMYA EYDTLFGQFVENEN+ KGSPPAAK++VENLPLVELK EN+L EE+V C
Sbjct: 324 YIADQDDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLAEELVAC 383

Query: 361 AVCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG 420
           AVCKDKFSMEEKVRKLPC HYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKS+RAG
Sbjct: 384 AVCKDKFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSRRAG 443

Query: 421 GGLQMDFQVRYNFEHIA 429
           GGLQ D +VRYNFE IA
Sbjct: 444 GGLQTDLRVRYNFELIA 455

BLAST of Moc04g37210.1 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 9.0e-27
Identity = 59/118 (50.00%), Postives = 75/118 (63.56%), Query Frame = 0

Query: 299 DYMYAAEYDTLFGQFVENE-NSKGSPPAAKTAVENLPLVELKMENVLTEEVVVCAVCKDK 358
           DY      + L  Q  EN+ N  G+PPA+K+A+E LPLV +   N L  E   CAVC D 
Sbjct: 172 DYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSN-LNSEFNQCAVCMDD 231

Query: 359 FSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYER--RKSQRAGGG 414
           F    + +++PC H YH DC+LPWL + N+CPVCR+ELPTDDPDYER  R +Q   GG
Sbjct: 232 FEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTSGG 288

BLAST of Moc04g37210.1 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.2e-26
Identity = 54/116 (46.55%), Postives = 77/116 (66.38%), Query Frame = 0

Query: 299 DYMYAAEYDTLFGQFVENE-NSKGSPPAAKTAVENLPLVELKMENVLTEEVVVCAVCKDK 358
           DY +    + L  Q  EN+ N  G+PPA+K+A++ LP V++  +++L  E+  CAVC D+
Sbjct: 164 DYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKV-TKDMLKSEMNQCAVCMDE 223

Query: 359 FSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGG 414
           F     V+++PC H +H DC+LPWL + N+CPVCR+ELPTDDPDYE R     G G
Sbjct: 224 FEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSG 278

BLAST of Moc04g37210.1 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.9e-25
Identity = 81/218 (37.16%), Postives = 110/218 (50.46%), Query Frame = 0

Query: 220 ISYE-DFEWEEVEER--VDDMENPSVVVDGAEELSLASGFSNEDESGEE-----ASRWEI 279
           +SYE DF   E+  R  +DD E+        EE       +  DE  EE      +R+ +
Sbjct: 100 LSYEDDFFRLELNSRNEIDDDEDEDEDDGDEEEEDEEENLTVNDEEDEEDDLRRRNRFPL 159

Query: 280 LLVMNDIGRN--TNWE------RDN------DSEAYIAEQDDYM-YAAEYDTLFGQFVEN 339
               +  GRN   +W        DN      +S+ Y     DY+  AA Y+ L     E 
Sbjct: 160 TTTQSRTGRNRILDWAEILMGIEDNSIEFRMESDRYAGNPADYIDDAAGYEALLQNLAEG 219

Query: 340 ENS-----KGSPPAAKTAVENLPLVELKMENVLTEEVVVCAVCKDKFSMEEKVRKLPCGH 399
           +       +G+PPAAK+A+E L   E+       E V+VCAVCKD   M E  +KLPCGH
Sbjct: 220 DGGGGGGRRGAPPAAKSAIEALETFEVSSSE--GEMVMVCAVCKDGMVMGETGKKLPCGH 279

Query: 400 YYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQR 410
            YH DCI+PWL  RN+CPVCR++L TDD +YE  + +R
Sbjct: 280 CYHGDCIVPWLGTRNSCPVCRFQLETDDAEYEEERKKR 315

BLAST of Moc04g37210.1 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 5.1e-22
Identity = 47/108 (43.52%), Postives = 70/108 (64.81%), Query Frame = 0

Query: 299 DYMYAAEYDTLFGQFVE-------NENSKGSPPAAKTAVENLPLVELKMENVLTEEVVVC 358
           +++  + +D L  Q  +       N  S   PPA+K+A+E LPL+E+   ++L++    C
Sbjct: 142 EFLLGSGFDRLLDQISQIELNTNRNLRSCEHPPASKSAIEALPLIEIDPTHLLSDSQSHC 201

Query: 359 AVCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDD 400
           AVCK+ F ++   R++PC H YH DCILPWL IRN+CPVCR+ELP +D
Sbjct: 202 AVCKENFVLKSSAREMPCNHIYHPDCILPWLAIRNSCPVCRHELPAED 249

BLAST of Moc04g37210.1 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 2.8e-20
Identity = 59/166 (35.54%), Postives = 90/166 (54.22%), Query Frame = 0

Query: 239 NPSVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEAYIAEQD 298
           NP +V+ G       +G   E E G+ A          D G  +      DS + I    
Sbjct: 95  NPVIVLQG------GAGEREEGEEGDAARDRRAFEFYYDDGSGSGLRPLPDSVSEI---- 154

Query: 299 DYMYAAEYDTLFGQFVENENS------KGSPPAAKTAVENLPLVELKMENVLTEEVVVCA 358
             +  + ++ L  Q  + E S       G+PPA+K+A+E+LP VE+   ++ +E    CA
Sbjct: 155 --LMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIGSE--ANCA 214

Query: 359 VCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTD 399
           VC + F  E + R++PC H +HDDCI+PWL+IRN+CPVCR+ELP++
Sbjct: 215 VCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSE 246

BLAST of Moc04g37210.1 vs. ExPASy TrEMBL
Match: A0A6J1BZP2 (uncharacterized protein LOC111006706 OS=Momordica charantia OX=3673 GN=LOC111006706 PE=4 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 4.1e-248
Identity = 428/428 (100.00%), Postives = 428/428 (100.00%), Query Frame = 0

Query: 1   MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSLLPNPMDFNPFAAVSSVVASADDSLP 60
           MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSLLPNPMDFNPFAAVSSVVASADDSLP
Sbjct: 1   MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSLLPNPMDFNPFAAVSSVVASADDSLP 60

Query: 61  FDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDRGSDPFTPSANDLDLGISG 120
           FDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDRGSDPFTPSANDLDLGISG
Sbjct: 61  FDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDRGSDPFTPSANDLDLGISG 120

Query: 121 FEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDVDSDSDSDN 180
           FEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDVDSDSDSDN
Sbjct: 121 FEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDVDSDSDSDN 180

Query: 181 GMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEERVDDMENP 240
           GMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEERVDDMENP
Sbjct: 181 GMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEERVDDMENP 240

Query: 241 SVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEAYIAEQDDY 300
           SVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEAYIAEQDDY
Sbjct: 241 SVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEAYIAEQDDY 300

Query: 301 MYAAEYDTLFGQFVENENSKGSPPAAKTAVENLPLVELKMENVLTEEVVVCAVCKDKFSM 360
           MYAAEYDTLFGQFVENENSKGSPPAAKTAVENLPLVELKMENVLTEEVVVCAVCKDKFSM
Sbjct: 301 MYAAEYDTLFGQFVENENSKGSPPAAKTAVENLPLVELKMENVLTEEVVVCAVCKDKFSM 360

Query: 361 EEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQMDFQV 420
           EEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQMDFQV
Sbjct: 361 EEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQMDFQV 420

Query: 421 RYNFEHIA 429
           RYNFEHIA
Sbjct: 421 RYNFEHIA 428

BLAST of Moc04g37210.1 vs. ExPASy TrEMBL
Match: A0A6J1JWL4 (uncharacterized protein LOC111488995 isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111488995 PE=4 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 6.2e-196
Identity = 351/437 (80.32%), Postives = 383/437 (87.64%), Query Frame = 0

Query: 1   MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSL----LPNPMDFNPFAAVSSVVASAD 60
           M + SIPKL  QPN+ LPD+T +D  SPVP+VPSL    LPN MDFNPFA +SS+V SAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDR----GSDPFTPSAN 120
           DS PFDTDWFSD+DS+SERDVSCFVTDLFENRRFTDHLHA  D DR    G DPFT SAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDV 180
             DLGISGFED+GEIES+YIEELWS FA+ESD+RVSE DVS   + VAD+   GLR+VDV
Sbjct: 121 --DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVS---MPVADSTTSGLRIVDV 180

Query: 181 DSDSDSDNGMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEE 240
           DSDS+SDNGM+GALQLVTGDD END NWVE+ NPLSD+FSFC QGQRI YEDFEWEEVEE
Sbjct: 181 DSDSESDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEE 240

Query: 241 RVDDMENPSVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEA 300
           RVD+ +N S VVDGAEELSLASGFSNE+ESGEEA RWEILLVMN+I RNTNWERDND+EA
Sbjct: 241 RVDERDNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNNIVRNTNWERDNDAEA 300

Query: 301 YIAEQDDYMYAAEYDTLFGQFVENENS-KGSPPAAKTAVENLPLVELKMENVLTEEVVVC 360
           YIA+QDDYMYA EYDTLFGQFVENEN+ KGSPPAAK++VENLPLVELK EN+LTEE+V C
Sbjct: 301 YIADQDDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVAC 360

Query: 361 AVCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG 420
           AVCKDKFSMEEKVRKLPC HYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG
Sbjct: 361 AVCKDKFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG 420

Query: 421 GGLQMDFQVRYNFEHIA 429
           GGLQ D +VRYNFE IA
Sbjct: 421 GGLQTDLRVRYNFELIA 432

BLAST of Moc04g37210.1 vs. ExPASy TrEMBL
Match: A0A6J1FE59 (uncharacterized protein LOC111444905 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111444905 PE=4 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 1.1e-195
Identity = 350/437 (80.09%), Postives = 383/437 (87.64%), Query Frame = 0

Query: 1   MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSL----LPNPMDFNPFAAVSSVVASAD 60
           M + SIPKL  QPN+ LPD+T +D  SPVP+VPSL    LPN MDFNPFA +SS+V SAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDR----GSDPFTPSAN 120
           DS PFDTDWFSD+DS+SERDVSCFVTDLFENRRFTDHLHA  D DR    G DPFT SAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDV 180
             DLGISGFED+GEIES+YIEELWS FA+ESD+RVSE DVS   + VAD+   GLR+VDV
Sbjct: 121 --DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVS---MPVADSTTSGLRIVDV 180

Query: 181 DSDSDSDNGMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEE 240
           DSDS+SDNGM+GALQLVTGDD END NWVE+ NPLSD+FSFC QGQRI YEDFEWEEVEE
Sbjct: 181 DSDSESDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEE 240

Query: 241 RVDDMENPSVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEA 300
           RVD+ +N S VVDGAEELSLASGFSNE+E+GEEA RWEILLVMNDI RNTNWERDND+EA
Sbjct: 241 RVDERDNLSAVVDGAEELSLASGFSNEEEAGEEAGRWEILLVMNDIVRNTNWERDNDAEA 300

Query: 301 YIAEQDDYMYAAEYDTLFGQFVENENS-KGSPPAAKTAVENLPLVELKMENVLTEEVVVC 360
           YIA+QDDYMYA EYDTLFGQFVENEN+ KGSPPAAK++VENLPLVELK EN+LTEE+V C
Sbjct: 301 YIADQDDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVAC 360

Query: 361 AVCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG 420
           AVCKDKFSMEEKVRKLPC HYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKS+RAG
Sbjct: 361 AVCKDKFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSRRAG 420

Query: 421 GGLQMDFQVRYNFEHIA 429
           GGLQ D +VRYNFE IA
Sbjct: 421 GGLQTDLRVRYNFELIA 432

BLAST of Moc04g37210.1 vs. ExPASy TrEMBL
Match: A0A6J1JYD4 (uncharacterized protein LOC111488995 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111488995 PE=4 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 2.9e-193
Identity = 346/433 (79.91%), Postives = 379/433 (87.53%), Query Frame = 0

Query: 1   MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSL----LPNPMDFNPFAAVSSVVASAD 60
           M + SIPKL  QPN+ LPD+T +D  SPVP+VPSL    LPN MDFNPFA +SS+V SAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDR----GSDPFTPSAN 120
           DS PFDTDWFSD+DS+SERDVSCFVTDLFENRRFTDHLHA  D DR    G DPFT SAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDV 180
             DLGISGFED+GEIES+YIEELWS FA+ESD+RVSE DVS   + VAD+   GLR+VDV
Sbjct: 121 --DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVS---MPVADSTTSGLRIVDV 180

Query: 181 DSDSDSDNGMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEE 240
           DSDS+SDNGM+GALQLVTGDD END NWVE+ NPLSD+FSFC QGQRI YEDFEWEEVEE
Sbjct: 181 DSDSESDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEE 240

Query: 241 RVDDMENPSVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEA 300
           RVD+ +N S VVDGAEELSLASGFSNE+ESGEEA RWEILLVMN+I RNTNWERDND+EA
Sbjct: 241 RVDERDNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNNIVRNTNWERDNDAEA 300

Query: 301 YIAEQDDYMYAAEYDTLFGQFVENENS-KGSPPAAKTAVENLPLVELKMENVLTEEVVVC 360
           YIA+QDDYMYA EYDTLFGQFVENEN+ KGSPPAAK++VENLPLVELK EN+LTEE+V C
Sbjct: 301 YIADQDDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVAC 360

Query: 361 AVCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG 420
           AVCKDKFSMEEKVRKLPC HYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG
Sbjct: 361 AVCKDKFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG 420

Query: 421 GGLQMDFQVRYNF 425
           GGLQ D +VR+ F
Sbjct: 421 GGLQTDLRVRFPF 428

BLAST of Moc04g37210.1 vs. ExPASy TrEMBL
Match: A0A6J1FKT6 (uncharacterized protein LOC111444905 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111444905 PE=4 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 4.9e-193
Identity = 345/433 (79.68%), Postives = 379/433 (87.53%), Query Frame = 0

Query: 1   MAYFSIPKLPHQPNMPLPDETDADCDSPVPTVPSL----LPNPMDFNPFAAVSSVVASAD 60
           M + SIPKL  QPN+ LPD+T +D  SPVP+VPSL    LPN MDFNPFA +SS+V SAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFDTDWFSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDR----GSDPFTPSAN 120
           DS PFDTDWFSD+DS+SERDVSCFVTDLFENRRFTDHLHA  D DR    G DPFT SAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSETDVSSMQVAVADAINGGLRLVDV 180
             DLGISGFED+GEIES+YIEELWS FA+ESD+RVSE DVS   + VAD+   GLR+VDV
Sbjct: 121 --DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVS---MPVADSTTSGLRIVDV 180

Query: 181 DSDSDSDNGMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEE 240
           DSDS+SDNGM+GALQLVTGDD END NWVE+ NPLSD+FSFC QGQRI YEDFEWEEVEE
Sbjct: 181 DSDSESDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEE 240

Query: 241 RVDDMENPSVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEA 300
           RVD+ +N S VVDGAEELSLASGFSNE+E+GEEA RWEILLVMNDI RNTNWERDND+EA
Sbjct: 241 RVDERDNLSAVVDGAEELSLASGFSNEEEAGEEAGRWEILLVMNDIVRNTNWERDNDAEA 300

Query: 301 YIAEQDDYMYAAEYDTLFGQFVENENS-KGSPPAAKTAVENLPLVELKMENVLTEEVVVC 360
           YIA+QDDYMYA EYDTLFGQFVENEN+ KGSPPAAK++VENLPLVELK EN+LTEE+V C
Sbjct: 301 YIADQDDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVAC 360

Query: 361 AVCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG 420
           AVCKDKFSMEEKVRKLPC HYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKS+RAG
Sbjct: 361 AVCKDKFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSRRAG 420

Query: 421 GGLQMDFQVRYNF 425
           GGLQ D +VR+ F
Sbjct: 421 GGLQTDLRVRFPF 428

BLAST of Moc04g37210.1 vs. TAIR 10
Match: AT3G02340.1 (RING/U-box superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 1.1e-40
Identity = 116/334 (34.73%), Postives = 175/334 (52.40%), Query Frame = 0

Query: 99  DPDRGSDP---FTPSANDLDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSE--TDVS 158
           +PD  S P   F   ++D+D+     E  G    +Y   +W  +  + D    E     S
Sbjct: 71  EPDPNSCPIDFFDRDSSDVDVA-EYLESGGLTAGDY--NIWGFYDPKEDEEEEEIVLGTS 130

Query: 159 SMQVAVADAINGGLRLVDVDSDSDSDNGMLGALQLVTGDDDENDVNWVESSNPLSDNFSF 218
              +   D+   GLR+  +DSDSD ++G+   +   +  +  ND   VE    L   +  
Sbjct: 131 GSDLQPGDSGEQGLRVTGIDSDSDCEDGVFDFISEDSSGNRGNDSGRVEVGTGLPPVWDH 190

Query: 219 CIQGQRISYEDFEWEEVEERVD--DMENPSVVVDGAEELSLASGFSNEDESGEEASRWEI 278
            + G+     D EWEEV+  ++      P    D  EE  L+S  S +DE  +    W++
Sbjct: 191 -LFGEGTVLADEEWEEVQNAINWTAFSGPE---DEDEEDELSS-LSRDDEEEDHELDWQV 250

Query: 279 LLVMNDIGRNTNW---------ERDNDSEAYIAEQDDY--MYAAEY--DTLFGQFVENEN 338
           LL +N++               + D D   Y++  D++   ++  Y  D + GQ  ++E 
Sbjct: 251 LLTVNNVVNYIEQAEGIMLNPDDIDPDYYLYLSGLDEFDENHSGHYDADAILGQMFDDET 310

Query: 339 S-KGSPPAAKTAVENLPLVELKMENVLTEEVVVCAVCKDKFSMEEKVRKLPCGHYYHDDC 398
             +G+PPAAK+ +++LP+VEL +E  L +   VCAVCKD+  +EEKVR+LPC H+YH +C
Sbjct: 311 GIRGNPPAAKSVIQDLPVVELAVEE-LDKGNNVCAVCKDEMLVEEKVRRLPCSHFYHGEC 370

Query: 399 ILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAG 412
           I+PWL IRNTCPVCRYELPTDD +YER KS   G
Sbjct: 371 IIPWLGIRNTCPVCRYELPTDDLEYERHKSSERG 395

BLAST of Moc04g37210.1 vs. TAIR 10
Match: AT5G08139.1 (RING/U-box superfamily protein )

HSP 1 Score: 149.1 bits (375), Expect = 8.3e-36
Identity = 108/295 (36.61%), Postives = 159/295 (53.90%), Query Frame = 0

Query: 142 ESDTRVSETDVSSMQVAVADAINGGLRLVDVDSDSDSDNGM----------LGALQLVTG 201
           + D  V  TD       +A + N  +RL+ V+S SD D+G+          L    +   
Sbjct: 82  DDDFFVGRTDFGLEFRDIASSGN-DIRLITVESGSDDDDGVENERELWGIDLNEEDVYVN 141

Query: 202 DDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEV----EERVDDME--------- 261
           DDDE + + V  + PL  + S  ++ + ++ ++F+WEEV       VDD           
Sbjct: 142 DDDEYEDDDVSVTIPLCWD-SLQLEDREVTADEFDWEEVGGGGGGGVDDEREIRSGFAQI 201

Query: 262 --NPSVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNTNWERDNDSEAYI-A 321
             N   ++  +  +SL    + E   G     WE+LL   +     N++ DN  E YI  
Sbjct: 202 DMNDESLISASPIISLEGLVTRERAEGSGNLGWEVLL---NHTLEINFDVDN-RELYIGG 261

Query: 322 EQDDYMYAAEYDTLFGQFVENENSK-GSPPAAKTAVENLPLVELKMENVLTEEVVVCAVC 381
           + DDY+   +YD LF QF + E S  G PP +K+ + NLP+V L+ EN   +  +VCAVC
Sbjct: 262 DHDDYV--QDYDMLFEQFADAEVSVIGLPPTSKSFLNNLPVVLLEGEND-DDGGLVCAVC 321

Query: 382 KDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQR 410
           KD+ ++  K  +LPC H YH +CI+PWL +RNTCPVCRYELPTDD +YE+RK+QR
Sbjct: 322 KDEMNIGNKAVQLPCNHKYHSECIVPWLKVRNTCPVCRYELPTDDAEYEQRKTQR 367

BLAST of Moc04g37210.1 vs. TAIR 10
Match: AT5G15820.1 (RING/U-box superfamily protein )

HSP 1 Score: 142.5 bits (358), Expect = 7.8e-34
Identity = 77/185 (41.62%), Postives = 108/185 (58.38%), Query Frame = 0

Query: 223 EDFEWEEVEERVDDMENPSVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRNT 282
           E  EWE     +  ++ P+      EE         E  +   +  WEILL  N      
Sbjct: 167 EVLEWENFNNAIHLVQEPAYASMEGEEEEEEDEVVMEFAASIYSDAWEILLYDN---MTN 226

Query: 283 NWERDNDSEAYIAEQDDYMYAAEYDTLFGQFVENENS-KGSPPAAKTAVENLPLVELKME 342
           +   D D E ++   D Y    +Y+ + GQ  +NE   KG+PPA+K+ V+ LP VEL +E
Sbjct: 227 SAPMDLDVEVWLDSVDGYA-PMDYNAIIGQMFDNETGIKGTPPASKSVVDGLPDVELTIE 286

Query: 343 NVLTEEVVVCAVCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPD 402
             L+   +VCA+CKD+   +EKV++LPC HYYH +CI+PWL IRNTCPVCR+ELPTDD +
Sbjct: 287 E-LSSVSIVCAICKDEVVFKEKVKRLPCKHYYHGECIIPWLGIRNTCPVCRHELPTDDLE 346

Query: 403 YERRK 407
           YER++
Sbjct: 347 YERKR 346

BLAST of Moc04g37210.1 vs. TAIR 10
Match: AT5G60820.1 (RING/U-box superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 4.9e-28
Identity = 121/373 (32.44%), Postives = 182/373 (48.79%), Query Frame = 0

Query: 66  FSDSDSDSERDVSCFVTDLFENRRFTDHLHALGDPDRGSDPFTPSANDLDLGISGF---- 125
           F+  D     D   FV DLF  R     +             TP  ND   GI  F    
Sbjct: 72  FTTDDGLDLLDRRSFVMDLFHQRVEQSQV-------------TPLDND---GIDDFENYE 131

Query: 126 --EDSGEIESNYIEELWSTFAVESDTRVSETD-VSSMQVAVADAINGGLRLVDVDSDSDS 185
             ED+ E++     E  + F V+SD  V+  + V  M++     I  G  +V V+S  DS
Sbjct: 132 MREDNVELDFGLEMESGNGF-VDSDLVVNLNEHVDGMRLL---GIESGDVIVTVESCFDS 191

Query: 186 DN------------GM-LGALQLVTGDDDENDVNWVESS--NPLSDNFSFCIQGQRISYE 245
           D+            G+ L        DDD+ND    ++S   PL  + S  ++   I+ E
Sbjct: 192 DDEDDQEKEESEIWGIDLNEEDEYVNDDDDNDDEEEDASVTIPLCWD-SLQLEDLGINNE 251

Query: 246 DFEWEEVEERVDDMENPSVVVDGAE----ELSLASGFSNED-----ESGEEASRWEILLV 305
           D EWEEV+   D+ E  SV+ +  +     +S+A+  S ED       G     WE+LL 
Sbjct: 252 DCEWEEVDSD-DEREVLSVLAEADDNNSVSVSVAATISLEDLAISERRGSSNLGWEVLLN 311

Query: 306 MNDIGRNTNWERDNDSEAYIAEQD-------DYMYAAEYDTLFGQFVENENSKGSPPAAK 365
              +  N + + +++ E YI + D       DY++  EY+ LF    E  +  G PPA+K
Sbjct: 312 SRSLEFNLD-DAESNLELYIGDIDHEEEDYEDYLHTTEYEMLFE--AEISSGIGKPPASK 371

Query: 366 TAVENLPLVELKMENVL--TEEVVVCAVCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIR 399
           + ++NL +  L  E+V+   ++ V CAVCK++  + ++V +LPC H YH +CI+PWL IR
Sbjct: 372 SFIKNLKVSPLSNEDVMENDDDAVCCAVCKEEMIVGKEVAELPCRHKYHSECIVPWLGIR 419

BLAST of Moc04g37210.1 vs. TAIR 10
Match: AT5G01980.1 (RING/U-box superfamily protein )

HSP 1 Score: 121.7 bits (304), Expect = 1.4e-27
Identity = 100/294 (34.01%), Postives = 146/294 (49.66%), Query Frame = 0

Query: 121 FEDSGEIESNYIEELWSTFAVESDT-----RVSETDVSSMQVAVADAINGGLRLVDVDSD 180
           F++ GE ESN    L+     ++DT        E+D S+ +   AD         D+D D
Sbjct: 147 FDNFGETESNASFSLYRFPHADNDTISFSAYGGESDASTDR--HADIFVQPDDRSDIDFD 206

Query: 181 SDSDNGMLGALQLVTGDDDENDVNWVESSNPLSDNFSFCIQGQRISYEDFEWEEVEERVD 240
           +D D    G  Q    + DE D  W E + P        + G R  Y ++          
Sbjct: 207 TDIDPMRAGLNQ---WNSDEEDREWEEGAGPSG------VAGTR--YRNY---------- 266

Query: 241 DMENPSVVVDGAEELSLASGFSNEDESGEEASRWEILLVMNDIGRN--TNWERDNDSEAY 300
            + +PS      E  S  + F + +   E + R  I+   + + RN  T  E D D   Y
Sbjct: 267 -LASPS------ESYSSMTRFDSPEL--ERSFRQRIIERRHSLSRNIFTGLE-DLDFSPY 326

Query: 301 IAEQDDYMYAAEYDTLFGQFVENENS-KGSPPAAKTAVENLPLVELKMENVLTEEVVVCA 360
            A   DY+    +D L  Q  E++NS +G+PPA+ + V NLP V +  E+V+  + +VCA
Sbjct: 327 AANVGDYLDERGFDELLEQLAESDNSRRGAPPASVSCVRNLPRVIIAEEHVM--KGLVCA 386

Query: 361 VCKDKFSMEEKVRKLPCGHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRK 407
           +CK+ FS+  +  +LPC H YH  CI+PWL+ RN+CP+CRYELPTDD DYE  K
Sbjct: 387 ICKELFSLRNETTQLPCLHLYHAHCIVPWLSARNSCPLCRYELPTDDKDYEEGK 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134467.18.4e-248100.00uncharacterized protein LOC111006706 [Momordica charantia][more]
XP_022992755.11.3e-19580.32uncharacterized protein LOC111488995 isoform X4 [Cucurbita maxima][more]
XP_023549550.12.2e-19580.09E3 ubiquitin ligase BIG BROTHER-related-like isoform X4 [Cucurbita pepo subsp. p... [more]
XP_022938801.12.2e-19580.09uncharacterized protein LOC111444905 isoform X3 [Cucurbita moschata][more]
KAG7016086.15.4e-19479.86E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
P0CH309.0e-2750.00E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q8LPN71.2e-2646.55E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q9SPL24.9e-2537.16E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV... [more]
O222835.1e-2243.52Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q940T52.8e-2035.54E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1BZP24.1e-248100.00uncharacterized protein LOC111006706 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A6J1JWL46.2e-19680.32uncharacterized protein LOC111488995 isoform X4 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FE591.1e-19580.09uncharacterized protein LOC111444905 isoform X3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JYD42.9e-19379.91uncharacterized protein LOC111488995 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FKT64.9e-19379.68uncharacterized protein LOC111444905 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT3G02340.11.1e-4034.73RING/U-box superfamily protein [more]
AT5G08139.18.3e-3636.61RING/U-box superfamily protein [more]
AT5G15820.17.8e-3441.62RING/U-box superfamily protein [more]
AT5G60820.14.9e-2832.44RING/U-box superfamily protein [more]
AT5G01980.11.4e-2734.01RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 351..391
e-value: 3.0E-8
score: 43.4
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 351..392
e-value: 5.2E-12
score: 45.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 351..392
score: 12.754832
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 311..398
e-value: 3.4E-22
score: 79.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR15710:SF108E3 UBIQUITIN-PROTEIN LIGASE CIP8-LIKE ISOFORM X1coord: 171..410
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 171..410
NoneNo IPR availableCDDcd16667RING-H2_RNF126_likecoord: 351..392
e-value: 5.64962E-24
score: 91.6104
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 340..398

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Moc04g37210Moc04g37210gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Moc04g37210.1.exon1Moc04g37210.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Moc04g37210.1cds.Moc04g37210.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Moc04g37210.1Moc04g37210.1-proteinpolypeptide