Moc04g08210.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g08210.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	E3 ubiquitin-protein ligase RING1-like
Location	chr4: 5944070 .. 5944798 (-)
Sequence length	729
RNA-Seq Expression	Moc04g08210.1
Synteny	Moc04g08210.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAATCAAAGTCAAAAGTGGCATCGATCCTGATCTTGAATTAATATCAATATCAATGGTGGAAAAAATCAGGCTGAAACTCCATGTACAGGCCCAATACGATCATTTCCAAGTCTTAGGGGAACATGCTCCAACATTGGTGAGCCATCGCCAAACCCCTACGTTTTGCTTCGCCGATTACCAACTTCCAGAGTGCATCTTCAACCGTGGCATTCGCAACAAGACTCTACAACGCTTCCTATCATTGCAATTCAAAGAATATGAAGACTCCATTAACATACAAGCCATGACACACCAAATAATAAATCATTGGATCCATGTCAAACGAGAAGCAGCTGCAACAGCAACTCATGATCATAATGATAATTCCAATTCCAGTGCATTTGGGAGAGTTTTTCCCCTTGAAGTTACCCTGCATTTGTGGAACTCAGTCATTCACTATGATGATGATGATTATTATGCTATGATTCCAGCAAGCGAAGTTGGTATTGAGAAGATGTTAAAGACCGTAACGTTTGAGGAGATTGCACCGGAATCAACAACTACTTGTGTCATTTGCTTGGAGGAGATCACCAACCAAGATATGGATAGTGGAATGTCATTAGTGGAGATGCCTTGCTTGCATGTGTTTCATGGAAGATGCATTAAGAAGTGGCTTAACATCAGTCATTATTGTCCCACTTGTAGATTTCAAATGCCTACCCATTCAACAAATTCTCATCAATAG

mRNA sequence

ATGTCAATCAAAGTCAAAAGTGGCATCGATCCTGATCTTGAATTAATATCAATATCAATGGTGGAAAAAATCAGGCTGAAACTCCATGTACAGGCCCAATACGATCATTTCCAAGTCTTAGGGGAACATGCTCCAACATTGGTGAGCCATCGCCAAACCCCTACGTTTTGCTTCGCCGATTACCAACTTCCAGAGTGCATCTTCAACCGTGGCATTCGCAACAAGACTCTACAACGCTTCCTATCATTGCAATTCAAAGAATATGAAGACTCCATTAACATACAAGCCATGACACACCAAATAATAAATCATTGGATCCATGTCAAACGAGAAGCAGCTGCAACAGCAACTCATGATCATAATGATAATTCCAATTCCAGTGCATTTGGGAGAGTTTTTCCCCTTGAAGTTACCCTGCATTTGTGGAACTCAGTCATTCACTATGATGATGATGATTATTATGCTATGATTCCAGCAAGCGAAGTTGGTATTGAGAAGATGTTAAAGACCGTAACGTTTGAGGAGATTGCACCGGAATCAACAACTACTTGTGTCATTTGCTTGGAGGAGATCACCAACCAAGATATGGATAGTGGAATGTCATTAGTGGAGATGCCTTGCTTGCATGTGTTTCATGGAAGATGCATTAAGAAGTGGCTTAACATCAGTCATTATTGTCCCACTTGTAGATTTCAAATGCCTACCCATTCAACAAATTCTCATCAATAG

Coding sequence (CDS)

ATGTCAATCAAAGTCAAAAGTGGCATCGATCCTGATCTTGAATTAATATCAATATCAATGGTGGAAAAAATCAGGCTGAAACTCCATGTACAGGCCCAATACGATCATTTCCAAGTCTTAGGGGAACATGCTCCAACATTGGTGAGCCATCGCCAAACCCCTACGTTTTGCTTCGCCGATTACCAACTTCCAGAGTGCATCTTCAACCGTGGCATTCGCAACAAGACTCTACAACGCTTCCTATCATTGCAATTCAAAGAATATGAAGACTCCATTAACATACAAGCCATGACACACCAAATAATAAATCATTGGATCCATGTCAAACGAGAAGCAGCTGCAACAGCAACTCATGATCATAATGATAATTCCAATTCCAGTGCATTTGGGAGAGTTTTTCCCCTTGAAGTTACCCTGCATTTGTGGAACTCAGTCATTCACTATGATGATGATGATTATTATGCTATGATTCCAGCAAGCGAAGTTGGTATTGAGAAGATGTTAAAGACCGTAACGTTTGAGGAGATTGCACCGGAATCAACAACTACTTGTGTCATTTGCTTGGAGGAGATCACCAACCAAGATATGGATAGTGGAATGTCATTAGTGGAGATGCCTTGCTTGCATGTGTTTCATGGAAGATGCATTAAGAAGTGGCTTAACATCAGTCATTATTGTCCCACTTGTAGATTTCAAATGCCTACCCATTCAACAAATTCTCATCAATAG

Protein sequence

MSIKVKSGIDPDLELISISMVEKIRLKLHVQAQYDHFQVLGEHAPTLVSHRQTPTFCFADYQLPECIFNRGIRNKTLQRFLSLQFKEYEDSINIQAMTHQIINHWIHVKREAAATATHDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYAMIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLVEMPCLHVFHGRCIKKWLNISHYCPTCRFQMPTHSTNSHQ

Homology

[Close]

BLAST of Moc04g08210.1 vs. NCBI nr
Match: XP_022158635.1 (RING-H2 finger protein ATL47-like [Momordica charantia])

HSP 1 Score: 332.4 bits (851), Expect = 3.2e-87
Identity = 159/190 (83.68%), Postives = 168/190 (88.42%), Query Frame = 0


Query: 56  FCFADYQLPECIFNRGIRNKTLQRFLSLQFKEYEDSINIQAMTHQIINHWIHVKREAAAT 115
           FCF DYQLPECI NRGI +KTLQRFLS QFKEYEDSINIQ MTHQIINHW+HVKRE  A 
Sbjct: 2   FCFVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVAA 61

Query: 116 ATHDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYAMIPASEVGIEKMLKTVTFEE 175
            T DHNDNSNS+AFGRVFPLEVTLHLWNSVIHYD D  YAM+PASEVGIEKMLKTV FEE
Sbjct: 62  TTQDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYD--YAMVPASEVGIEKMLKTVKFEE 121

Query: 176 IAPESTTTCVICLEEIT---NQDMDSGMSLVEMPCLHVFHGRCIKKWLNISHYCPTCRFQ 235
           I  ES TTCVICLEEIT   NQD+DSGM L+++PCLHVFHGRCIKKWLN SHYCPTCRFQ
Sbjct: 122 IVLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHYCPTCRFQ 181

Query: 236 MPTHSTNSHQ 243
           MPT+STNSHQ
Sbjct: 182 MPTNSTNSHQ 189

[Close]

BLAST of Moc04g08210.1 vs. NCBI nr
Match: XP_022158633.1 (E3 ubiquitin-protein ligase RING1-like [Momordica charantia])

HSP 1 Score: 237.3 bits (604), Expect = 1.4e-58
Identity = 119/148 (80.41%), Postives = 127/148 (85.81%), Query Frame = 0


Query: 97  MTHQIINHWIHVKREAAATA-THDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYA 156
           MT QIINHW+HVKREAAA A T D N+NSNS AFGRVFPLEVTLHLWNSVI  DDDD YA
Sbjct: 1   MTXQIINHWLHVKREAAAAATTRDRNNNSNSIAFGRVFPLEVTLHLWNSVIE-DDDDDYA 60

Query: 157 MIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEIT---NQDMDSGMSLVEMPCLHVFH 216
           M+PASEVGIEKMLKTV FEEI  ES TTCVICLEEIT   NQD+DSGM LV+MPCLHVFH
Sbjct: 61  MVPASEVGIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLVQMPCLHVFH 120

Query: 217 GRCIKKWLNISHYCPTCRFQMPTHSTNS 241
            +CI KWLN SHYCPTCRFQMPT+ TN+
Sbjct: 121 KKCILKWLNNSHYCPTCRFQMPTNLTNA 147

[Close]

BLAST of Moc04g08210.1 vs. NCBI nr
Match: XP_022158634.1 (E3 ubiquitin-protein ligase RING1-like [Momordica charantia])

HSP 1 Score: 223.8 bits (569), Expect = 1.6e-54
Identity = 112/144 (77.78%), Postives = 121/144 (84.03%), Query Frame = 0


Query: 97  MTHQIINHWIHVKRE-AAATATHDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYA 156
           MTHQIINHW+HVKRE AAA+AT DHN+NSNS AFGRVF LEVT HLWNSVI  DDDD YA
Sbjct: 1   MTHQIINHWLHVKREAAAASATRDHNNNSNSIAFGRVFSLEVTFHLWNSVIE-DDDDDYA 60

Query: 157 MIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEIT---NQDMDSGMSLVEMPCLHVFH 216
           M+PASEV IEKMLKTV FEEI  ES TTCVICLEEIT   NQD+D+GM LV +PCLHVFH
Sbjct: 61  MVPASEVAIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDNGMLLVHIPCLHVFH 120

Query: 217 GRCIKKWLNISHYCPTCRFQMPTH 237
             CI KWLN SHYCPTCR +MPT+
Sbjct: 121 HICILKWLNNSHYCPTCRCEMPTN 143

[Close]

BLAST of Moc04g08210.1 vs. NCBI nr
Match: ADN34252.1 (hypothetical zinc finger protein [Cucumis melo subsp. melo] >KAA0031412.1 putative zinc finger protein [Cucumis melo var. makuwa] >TYK06863.1 putative zinc finger protein [Cucumis melo var. makuwa])

HSP 1 Score: 163.3 bits (412), Expect = 2.6e-36
Identity = 91/230 (39.57%), Postives = 131/230 (56.96%), Query Frame = 0


Query: 17  SISMVEKIRLKLHVQAQYDHFQVL---GEHAPTLVSHRQTPTFCFADYQLPECIFNRGIR 76
           S ++VE+I LK  VQ +  HFQV      H+ TLV         FA +QLP  IF  G  
Sbjct: 41  SPTLVEQICLKFKVQLKCQHFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHG-- 100

Query: 77  NKTLQRFLSLQFKEYEDSINIQAMTHQIINHWIHVKREAAATATHDHNDNSNSSAFGRVF 136
           +KTL+R L  +FK Y D INI+ + ++II HW+    E       +  +N+++  F +++
Sbjct: 101 DKTLKRILFREFKMYGDIINIELLANEIIKHWVRKVEE------EEDQENNSNVLFKKIY 160

Query: 137 PLEVTLHLWNSVI------HYDDDDYYAMIPASEVGIEKMLKTVTFEEI---APESTTTC 196
           PLE+ + L  S +           D   M+P S+  +E MLK V  EEI     + +  C
Sbjct: 161 PLEIMIRLLVSKMIRAVQPRLAMIDQPLMVPTSDSAVESMLKRVENEEIMKSGDDESINC 220

Query: 197 VICLEEITNQDMDSGMSLVEMPCLHVFHGRCIKKWLNISHYCPTCRFQMP 235
           V+CLEEI+ ++  S  ++++MPCLH+FH  CI+KWL  SHYCPTCRF MP
Sbjct: 221 VVCLEEISKEEKGSETTVLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMP 262

[Close]

BLAST of Moc04g08210.1 vs. NCBI nr
Match: KGN43799.1 (hypothetical protein Csa_017222 [Cucumis sativus])

HSP 1 Score: 149.4 bits (376), Expect = 3.9e-32
Identity = 101/266 (37.97%), Postives = 139/266 (52.26%), Query Frame = 0


Query: 1   MSIKVKSGIDPDLEL----------ISISMVEKIRLKLHVQAQYDHFQVLGE---HAPTL 60
           MS+++    DPDL L           S + V++I LKL V+ +  HFQ   E    A TL
Sbjct: 18  MSLRI---TDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQHFQADEEGDTFASTL 77

Query: 61  -----VSHRQTPTFCFADYQLPECIFNRGIRNKTLQRFLSLQFKEYEDSINIQAMTHQII 120
                VSH   PTF     QLP  +F  G  N+TL+R L  QF+ +   INIQ +  +II
Sbjct: 78  MIEHIVSHHPFPTF-----QLPVSVFRHG--NRTLKRLLFQQFQIFRGIINIQLVADEII 137

Query: 121 NHWIHVKREAAATATHDHNDNSNSSAFGRVFPLEVT--------LHLWNSVIHYDDDDYY 180
            HW  VK+E       +  +N++      ++PLE+T        +H  +      D    
Sbjct: 138 EHW--VKKE-------EDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQV 197

Query: 181 AMIPASEVGIEKMLKTVTFEEI---APESTTTCVICLEEITNQDMDSGMSLVEMPCLHVF 238
            M+PASE  +E MLK V  EEI     + +  CVICLEEI+ +   S   +++MPCLH+F
Sbjct: 198 TMVPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKMKGSEGVVLQMPCLHMF 257

[Close]

BLAST of Moc04g08210.1 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 9.7e-10
Identity = 27/87 (31.03%), Postives = 46/87 (52.87%), Query Frame = 0


Query: 149 DDDDYYAMIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLVEMPCL 208
           +D + Y   PAS+  I+ +      +++       C +C++E      + G  + +MPC 
Sbjct: 181 NDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDE-----FEDGSDVKQMPCK 240

Query: 209 HVFHGRCIKKWLNISHYCPTCRFQMPT 236
           HVFH  C+  WL + + CP CRF++PT
Sbjct: 241 HVFHQDCLLPWLELHNSCPVCRFELPT 262

[Close]

BLAST of Moc04g08210.1 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.7e-09
Identity = 30/78 (38.46%), Postives = 44/78 (56.41%), Query Frame = 0


Query: 158 PASEVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLVEMPCLHVFHGRCIK 217
           PAS+  IE  L  V   +   ++   C +C E       ++G+   EMPC H+FHG CI 
Sbjct: 190 PASKSAIES-LPRVEISDCHTKAEANCAVCTEV-----FEAGIEGREMPCKHIFHGDCIV 249

Query: 218 KWLNISHYCPTCRFQMPT 236
            WL+I + CP CRF++P+
Sbjct: 250 PWLSIRNSCPVCRFELPS 261

[Close]

BLAST of Moc04g08210.1 vs. ExPASy Swiss-Prot
Match: Q8GXF8 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SGR9 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.7e-09
Identity = 34/95 (35.79%), Postives = 47/95 (49.47%), Query Frame = 0


Query: 142 WNSVIHYDDDDYYAMIPASEVGIEKMLKTVTFEEIAPES-TTTCVICLEEITNQDMDSGM 201
           +N +   D  + Y  +PA+   +  +     F   A  +    CVIC EE     M  G 
Sbjct: 172 YNHLRRRDQKEGY-QVPAARAAVVALRAVEVFNVAASNAGEVECVICKEE-----MSEGR 231

Query: 202 SLVEMPCLHVFHGRCIKKWLNISHYCPTCRFQMPT 236
            + EMPC H FH +CI  WL+  + CP CRFQ+PT
Sbjct: 232 DVCEMPCQHFFHWKCILPWLSKKNTCPFCRFQLPT 260

[Close]

BLAST of Moc04g08210.1 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.1e-08
Identity = 33/77 (42.86%), Postives = 44/77 (57.14%), Query Frame = 0


Query: 158 PASEVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLVEMPCLHVFHGRCIK 217
           PAS+  I+ M   V  +    E    CVICLEE  +++     ++ EMPC H FHG CI+
Sbjct: 92  PASKASIDAM-PIVEIDGCEGE----CVICLEEWKSEE-----TVKEMPCKHRFHGGCIE 151

Query: 218 KWLNISHYCPTCRFQMP 235
           KWL     CP CR++MP
Sbjct: 152 KWLGFHGSCPVCRYEMP 158

[Close]

BLAST of Moc04g08210.1 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.1e-08
Identity = 36/120 (30.00%), Postives = 53/120 (44.17%), Query Frame = 0


Query: 119 DHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYAMIPASEVGIEKMLKTVTFEEIAP 178
           D   ++N       + L   L L    +   D       PA +  +E  L TV  EE+  
Sbjct: 140 DQGGSTNDDGLLEEYVLGAGLSLLLQHLAESDPSRNGTPPAKKEAVE-ALPTVKIEEV-- 199

Query: 179 ESTTTCVICLEEITNQDMDSGMSLVEMPCLHVFHGRCIKKWLNISHYCPTCRFQMPTHST 238
               +C +CL+     D++ G    +MPC H FH  CI  WL +   CP CRF++P+  T
Sbjct: 200 ---VSCSVCLD-----DLEVGSQAKQMPCEHKFHSSCILPWLELHSSCPVCRFELPSEET 248

[Close]

BLAST of Moc04g08210.1 vs. ExPASy TrEMBL
Match: A0A6J1DWD8 (RING-H2 finger protein ATL47-like OS=Momordica charantia OX=3673 GN=LOC111025085 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.6e-87
Identity = 159/190 (83.68%), Postives = 168/190 (88.42%), Query Frame = 0


Query: 56  FCFADYQLPECIFNRGIRNKTLQRFLSLQFKEYEDSINIQAMTHQIINHWIHVKREAAAT 115
           FCF DYQLPECI NRGI +KTLQRFLS QFKEYEDSINIQ MTHQIINHW+HVKRE  A 
Sbjct: 2   FCFVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVAA 61

Query: 116 ATHDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYAMIPASEVGIEKMLKTVTFEE 175
            T DHNDNSNS+AFGRVFPLEVTLHLWNSVIHYD D  YAM+PASEVGIEKMLKTV FEE
Sbjct: 62  TTQDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYD--YAMVPASEVGIEKMLKTVKFEE 121

Query: 176 IAPESTTTCVICLEEIT---NQDMDSGMSLVEMPCLHVFHGRCIKKWLNISHYCPTCRFQ 235
           I  ES TTCVICLEEIT   NQD+DSGM L+++PCLHVFHGRCIKKWLN SHYCPTCRFQ
Sbjct: 122 IVLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHYCPTCRFQ 181

Query: 236 MPTHSTNSHQ 243
           MPT+STNSHQ
Sbjct: 182 MPTNSTNSHQ 189

[Close]

BLAST of Moc04g08210.1 vs. ExPASy TrEMBL
Match: A0A6J1DWN3 (E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC111025082 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 6.8e-59
Identity = 119/148 (80.41%), Postives = 127/148 (85.81%), Query Frame = 0


Query: 97  MTHQIINHWIHVKREAAATA-THDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYA 156
           MT QIINHW+HVKREAAA A T D N+NSNS AFGRVFPLEVTLHLWNSVI  DDDD YA
Sbjct: 1   MTXQIINHWLHVKREAAAAATTRDRNNNSNSIAFGRVFPLEVTLHLWNSVIE-DDDDDYA 60

Query: 157 MIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEIT---NQDMDSGMSLVEMPCLHVFH 216
           M+PASEVGIEKMLKTV FEEI  ES TTCVICLEEIT   NQD+DSGM LV+MPCLHVFH
Sbjct: 61  MVPASEVGIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLVQMPCLHVFH 120

Query: 217 GRCIKKWLNISHYCPTCRFQMPTHSTNS 241
            +CI KWLN SHYCPTCRFQMPT+ TN+
Sbjct: 121 KKCILKWLNNSHYCPTCRFQMPTNLTNA 147

[Close]

BLAST of Moc04g08210.1 vs. ExPASy TrEMBL
Match: A0A6J1DZZ7 (E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC111025083 PE=4 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 7.8e-55
Identity = 112/144 (77.78%), Postives = 121/144 (84.03%), Query Frame = 0


Query: 97  MTHQIINHWIHVKRE-AAATATHDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYA 156
           MTHQIINHW+HVKRE AAA+AT DHN+NSNS AFGRVF LEVT HLWNSVI  DDDD YA
Sbjct: 1   MTHQIINHWLHVKREAAAASATRDHNNNSNSIAFGRVFSLEVTFHLWNSVIE-DDDDDYA 60

Query: 157 MIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEIT---NQDMDSGMSLVEMPCLHVFH 216
           M+PASEV IEKMLKTV FEEI  ES TTCVICLEEIT   NQD+D+GM LV +PCLHVFH
Sbjct: 61  MVPASEVAIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDNGMLLVHIPCLHVFH 120

Query: 217 GRCIKKWLNISHYCPTCRFQMPTH 237
             CI KWLN SHYCPTCR +MPT+
Sbjct: 121 HICILKWLNNSHYCPTCRCEMPTN 143

[Close]

BLAST of Moc04g08210.1 vs. ExPASy TrEMBL
Match: A0A5D3C4K6 (Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001670 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 1.3e-36
Identity = 91/230 (39.57%), Postives = 131/230 (56.96%), Query Frame = 0


Query: 17  SISMVEKIRLKLHVQAQYDHFQVL---GEHAPTLVSHRQTPTFCFADYQLPECIFNRGIR 76
           S ++VE+I LK  VQ +  HFQV      H+ TLV         FA +QLP  IF  G  
Sbjct: 41  SPTLVEQICLKFKVQLKCQHFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHG-- 100

Query: 77  NKTLQRFLSLQFKEYEDSINIQAMTHQIINHWIHVKREAAATATHDHNDNSNSSAFGRVF 136
           +KTL+R L  +FK Y D INI+ + ++II HW+    E       +  +N+++  F +++
Sbjct: 101 DKTLKRILFREFKMYGDIINIELLANEIIKHWVRKVEE------EEDQENNSNVLFKKIY 160

Query: 137 PLEVTLHLWNSVI------HYDDDDYYAMIPASEVGIEKMLKTVTFEEI---APESTTTC 196
           PLE+ + L  S +           D   M+P S+  +E MLK V  EEI     + +  C
Sbjct: 161 PLEIMIRLLVSKMIRAVQPRLAMIDQPLMVPTSDSAVESMLKRVENEEIMKSGDDESINC 220

Query: 197 VICLEEITNQDMDSGMSLVEMPCLHVFHGRCIKKWLNISHYCPTCRFQMP 235
           V+CLEEI+ ++  S  ++++MPCLH+FH  CI+KWL  SHYCPTCRF MP
Sbjct: 221 VVCLEEISKEEKGSETTVLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMP 262

[Close]

BLAST of Moc04g08210.1 vs. ExPASy TrEMBL
Match: E5GCQ3 (Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 1.3e-36
Identity = 91/230 (39.57%), Postives = 131/230 (56.96%), Query Frame = 0


Query: 17  SISMVEKIRLKLHVQAQYDHFQVL---GEHAPTLVSHRQTPTFCFADYQLPECIFNRGIR 76
           S ++VE+I LK  VQ +  HFQV      H+ TLV         FA +QLP  IF  G  
Sbjct: 41  SPTLVEQICLKFKVQLKCQHFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHG-- 100

Query: 77  NKTLQRFLSLQFKEYEDSINIQAMTHQIINHWIHVKREAAATATHDHNDNSNSSAFGRVF 136
           +KTL+R L  +FK Y D INI+ + ++II HW+    E       +  +N+++  F +++
Sbjct: 101 DKTLKRILFREFKMYGDIINIELLANEIIKHWVRKVEE------EEDQENNSNVLFKKIY 160

Query: 137 PLEVTLHLWNSVI------HYDDDDYYAMIPASEVGIEKMLKTVTFEEI---APESTTTC 196
           PLE+ + L  S +           D   M+P S+  +E MLK V  EEI     + +  C
Sbjct: 161 PLEIMIRLLVSKMIRAVQPRLAMIDQPLMVPTSDSAVESMLKRVENEEIMKSGDDESINC 220

Query: 197 VICLEEITNQDMDSGMSLVEMPCLHVFHGRCIKKWLNISHYCPTCRFQMP 235
           V+CLEEI+ ++  S  ++++MPCLH+FH  CI+KWL  SHYCPTCRF MP
Sbjct: 221 VVCLEEISKEEKGSETTVLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMP 262

[Close]

BLAST of Moc04g08210.1 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 6.9e-11
Identity = 27/87 (31.03%), Postives = 46/87 (52.87%), Query Frame = 0


Query: 149 DDDDYYAMIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLVEMPCL 208
           +D + Y   PAS+  I+ +      +++       C +C++E      + G  + +MPC 
Sbjct: 181 NDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDE-----FEDGSDVKQMPCK 240

Query: 209 HVFHGRCIKKWLNISHYCPTCRFQMPT 236
           HVFH  C+  WL + + CP CRF++PT
Sbjct: 241 HVFHQDCLLPWLELHNSCPVCRFELPT 262

[Close]

BLAST of Moc04g08210.1 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.6e-10
Identity = 30/78 (38.46%), Postives = 44/78 (56.41%), Query Frame = 0


Query: 158 PASEVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLVEMPCLHVFHGRCIK 217
           PAS+  IE  L  V   +   ++   C +C E       ++G+   EMPC H+FHG CI 
Sbjct: 190 PASKSAIES-LPRVEISDCHTKAEANCAVCTEV-----FEAGIEGREMPCKHIFHGDCIV 249

Query: 218 KWLNISHYCPTCRFQMPT 236
            WL+I + CP CRF++P+
Sbjct: 250 PWLSIRNSCPVCRFELPS 261

[Close]

BLAST of Moc04g08210.1 vs. TAIR 10
Match: AT5G02750.1 (RING/U-box superfamily protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.6e-10
Identity = 34/95 (35.79%), Postives = 47/95 (49.47%), Query Frame = 0


Query: 142 WNSVIHYDDDDYYAMIPASEVGIEKMLKTVTFEEIAPES-TTTCVICLEEITNQDMDSGM 201
           +N +   D  + Y  +PA+   +  +     F   A  +    CVIC EE     M  G 
Sbjct: 172 YNHLRRRDQKEGY-QVPAARAAVVALRAVEVFNVAASNAGEVECVICKEE-----MSEGR 231

Query: 202 SLVEMPCLHVFHGRCIKKWLNISHYCPTCRFQMPT 236
            + EMPC H FH +CI  WL+  + CP CRFQ+PT
Sbjct: 232 DVCEMPCQHFFHWKCILPWLSKKNTCPFCRFQLPT 260

[Close]

BLAST of Moc04g08210.1 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 7.6e-10
Identity = 33/77 (42.86%), Postives = 44/77 (57.14%), Query Frame = 0


Query: 158 PASEVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLVEMPCLHVFHGRCIK 217
           PAS+  I+ M   V  +    E    CVICLEE  +++     ++ EMPC H FHG CI+
Sbjct: 92  PASKASIDAM-PIVEIDGCEGE----CVICLEEWKSEE-----TVKEMPCKHRFHGGCIE 151

Query: 218 KWLNISHYCPTCRFQMP 235
           KWL     CP CR++MP
Sbjct: 152 KWLGFHGSCPVCRYEMP 158

[Close]

BLAST of Moc04g08210.1 vs. TAIR 10
Match: AT3G13228.1 (RING/U-box superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 4.9e-09
Identity = 35/134 (26.12%), Postives = 70/134 (52.24%), Query Frame = 0


Query: 101 IINHWIHVKREAAATATHDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYAMIPAS 160
           +I H ++V +   ++A++ H+          +FP + T       + +D+   + + PAS
Sbjct: 195 VIYHILYVIK-ITSSASNGHSPGCALQVSLYLFPHDETDIEEAVQVSFDETTNFCLGPAS 254

Query: 161 EVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLVEMPCLHVFHGRCIKKWL 220
           ++ ++ + + + +++I   +   C ICLEE      ++G  LV +PC H F   C  KW 
Sbjct: 255 KLVVKSLTRKI-YDKI-NYTGERCTICLEE-----FNAGGILVALPCGHDFDDECAVKWF 314

Query: 221 NISHYCPTCRFQMP 235
             +H+CP CR+++P
Sbjct: 315 ETNHFCPLCRYELP 320

The following BLAST results are available for this feature:

BLAST of Moc04g08210.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158635.1	3.2e-87	83.68	RING-H2 finger protein ATL47-like [Momordica charantia]	[more]
XP_022158633.1	1.4e-58	80.41	E3 ubiquitin-protein ligase RING1-like [Momordica charantia]	[more]
XP_022158634.1	1.6e-54	77.78	E3 ubiquitin-protein ligase RING1-like [Momordica charantia]	[more]
ADN34252.1	2.6e-36	39.57	hypothetical zinc finger protein [Cucumis melo subsp. melo] >KAA0031412.1 putati...	[more]
KGN43799.1	3.9e-32	37.97	hypothetical protein Csa_017222 [Cucumis sativus]	[more]

BLAST of Moc04g08210.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	9.7e-10	31.03	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q9SNB6	3.7e-09	38.46	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
Q8GXF8	3.7e-09	35.79	E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 G...	[more]
Q9LQX2	1.1e-08	42.86	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
Q6AVN2	1.1e-08	30.00	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]

BLAST of Moc04g08210.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWD8	1.6e-87	83.68	RING-H2 finger protein ATL47-like OS=Momordica charantia OX=3673 GN=LOC111025085...	[more]
A0A6J1DWN3	6.8e-59	80.41	E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1DZZ7	7.8e-55	77.78	E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5D3C4K6	1.3e-36	39.57	Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
E5GCQ3	1.3e-36	39.57	Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]

BLAST of Moc04g08210.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	6.9e-11	31.03	RING/U-box superfamily protein	[more]
AT3G46620.1	2.6e-10	38.46	zinc finger (C3HC4-type RING finger) family protein	[more]
AT5G02750.1	2.6e-10	35.79	RING/U-box superfamily protein	[more]
AT1G26800.1	7.6e-10	42.86	RING/U-box superfamily protein	[more]
AT3G13228.1	4.9e-09	26.12	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 184..229 e-value: 1.4E-7 score: 41.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 183..230 e-value: 1.8E-11 score: 44.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 184..230 score: 12.769585
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 183..230 e-value: 0.0057 score: 9.2
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 147..236 e-value: 3.3E-18 score: 67.2
None	No IPR available	PANTHER	PTHR15710:SF77	OS07G0631200 PROTEIN	coord: 114..234
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 114..234
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 166..235

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc04g08210	Moc04g08210	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc04g08210.1.exon1	Moc04g08210.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc04g08210.1	cds.Moc04g08210.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc04g08210.1	Moc04g08210.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008270	zinc ion binding