Moc01g33220.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g33220.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	gibberellin 2-beta-dioxygenase-like
Location	chr1: 23341913 .. 23342975 (+)
Sequence length	978
RNA-Seq Expression	Moc01g33220.1
Synteny	Moc01g33220.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGTTCTGTCACAGCCAGCATTGGAGTTGGACCACAATTCCCTTCTACGAACATGCAGAGCCACCGCCTCCTCCGCCGATATTCCCGAAATCGACCTCTCCCACGCCGACGCCAAGTCCCGCATCGTCAAGGCCTTCGAAGAATTCGGATTCTGTAAGCTCATCAACCACACAATTCCCATGGACTCCATTACTAAACTGGAACAGGAAGCTCTCTGTTTCTTCAAACTTCCCCAATCGGAGAAAGAAAAATCTCTCCCCCCCGACCCTTTAGGCTACGGCAGCAAAAACATCGGCCCCAATGGCGACGAGGGCTGGATTGAATACTTCCTCCTCAACGCCAATCCTCTCCCCATTCCCTCCGGCAACCATCAATCTCTGCTGTAAGTTCCCGAACAGAGTCCTCTGTTTTTCTAGAATTTTATTTATTGGGAAATTTTAAGTTTGACATTTTGAGGGCGTGTGCAGCGCCGCCGTAAACGAGTACGTCCCGGCGGTGAAGAAATTGGGGAGTGAAATCGTGGAGCTGATAACTGAAGGATTGAATGTGAAGGCAAGAAACGCGGTGAGCAGGCTTCTGAACGACGAGAAGAGCGACTCGTGTTTCAGAGTGAACCACTATCCGCCGTGGCCGGAGGAGGGAGCAGCCATGGGCGCGCGGAACTTGCTCGGTTTCGGGGAGCACACGGACCCACACATAATATCGCTGCTGAGATCGAACAACTCGGCGGGGCTGCAAATCCGGCTGCGAGACGGCGCGTGGGTGGCAGTGCCGGCGGACGAGGCGGCGTTCTTCGTGAACGTGGGCGACGCGCTGCAGGTGATGAGCAACGGGAGGTTCAGGAGCGTGGTGCACAAGGTGGTGGCGGGGGCGGCCAAGGAGAGGGTTTCGATGATTTATTTCGCCGGGCCGCCGCCGAGTGAGAGGATCGCGCCGCTGCCGGAGGTTTTGGGGGACGGGGAAGAAAGCTTGTACAAGGAATTCACGTGGTGGGAATACAAAACGGCGGCGTATAAGACCAGATTGGCTGATTACAGGCTGGGTTTGTTTGAGAAGTGA

mRNA sequence

ATGGTTGTTCTGTCACAGCCAGCATTGGAGTTGGACCACAATTCCCTTCTACGAACATGCAGAGCCACCGCCTCCTCCGCCGATATTCCCGAAATCGACCTCTCCCACGCCGACGCCAAGTCCCGCATCGTCAAGGCCTTCGAAGAATTCGGATTCTGTAAGCTCATCAACCACACAATTCCCATGGACTCCATTACTAAACTGGAACAGGAAGCTCTCTGTTTCTTCAAACTTCCCCAATCGGAGAAAGAAAAATCTCTCCCCCCCGACCCTTTAGGCTACGGCAGCAAAAACATCGGCCCCAATGGCGACGAGGGCTGGATTGAATACTTCCTCCTCAACGCCAATCCTCTCCCCATTCCCTCCGGCAACCATCAATCTCTGCTCGCCGCCGTAAACGAGTACGTCCCGGCGGTGAAGAAATTGGGGAGTGAAATCGTGGAGCTGATAACTGAAGGATTGAATGTGAAGGCAAGAAACGCGGTGAGCAGGCTTCTGAACGACGAGAAGAGCGACTCGTGTTTCAGAGTGAACCACTATCCGCCGTGGCCGGAGGAGGGAGCAGCCATGGGCGCGCGGAACTTGCTCGGTTTCGGGGAGCACACGGACCCACACATAATATCGCTGCTGAGATCGAACAACTCGGCGGGGCTGCAAATCCGGCTGCGAGACGGCGCGTGGGTGGCAGTGCCGGCGGACGAGGCGGCGTTCTTCGTGAACGTGGGCGACGCGCTGCAGGTGATGAGCAACGGGAGGTTCAGGAGCGTGGTGCACAAGGTGGTGGCGGGGGCGGCCAAGGAGAGGGTTTCGATGATTTATTTCGCCGGGCCGCCGCCGAGTGAGAGGATCGCGCCGCTGCCGGAGGTTTTGGGGGACGGGGAAGAAAGCTTGTACAAGGAATTCACGTGGTGGGAATACAAAACGGCGGCGTATAAGACCAGATTGGCTGATTACAGGCTGGGTTTGTTTGAGAAGTGA

Coding sequence (CDS)

ATGGTTGTTCTGTCACAGCCAGCATTGGAGTTGGACCACAATTCCCTTCTACGAACATGCAGAGCCACCGCCTCCTCCGCCGATATTCCCGAAATCGACCTCTCCCACGCCGACGCCAAGTCCCGCATCGTCAAGGCCTTCGAAGAATTCGGATTCTGTAAGCTCATCAACCACACAATTCCCATGGACTCCATTACTAAACTGGAACAGGAAGCTCTCTGTTTCTTCAAACTTCCCCAATCGGAGAAAGAAAAATCTCTCCCCCCCGACCCTTTAGGCTACGGCAGCAAAAACATCGGCCCCAATGGCGACGAGGGCTGGATTGAATACTTCCTCCTCAACGCCAATCCTCTCCCCATTCCCTCCGGCAACCATCAATCTCTGCTCGCCGCCGTAAACGAGTACGTCCCGGCGGTGAAGAAATTGGGGAGTGAAATCGTGGAGCTGATAACTGAAGGATTGAATGTGAAGGCAAGAAACGCGGTGAGCAGGCTTCTGAACGACGAGAAGAGCGACTCGTGTTTCAGAGTGAACCACTATCCGCCGTGGCCGGAGGAGGGAGCAGCCATGGGCGCGCGGAACTTGCTCGGTTTCGGGGAGCACACGGACCCACACATAATATCGCTGCTGAGATCGAACAACTCGGCGGGGCTGCAAATCCGGCTGCGAGACGGCGCGTGGGTGGCAGTGCCGGCGGACGAGGCGGCGTTCTTCGTGAACGTGGGCGACGCGCTGCAGGTGATGAGCAACGGGAGGTTCAGGAGCGTGGTGCACAAGGTGGTGGCGGGGGCGGCCAAGGAGAGGGTTTCGATGATTTATTTCGCCGGGCCGCCGCCGAGTGAGAGGATCGCGCCGCTGCCGGAGGTTTTGGGGGACGGGGAAGAAAGCTTGTACAAGGAATTCACGTGGTGGGAATACAAAACGGCGGCGTATAAGACCAGATTGGCTGATTACAGGCTGGGTTTGTTTGAGAAGTGA

Protein sequence

MVVLSQPALELDHNSLLRTCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHTIPMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLPIPSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNHYPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFVNVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYKEFTWWEYKTAAYKTRLADYRLGLFEK

Homology

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BLAST of Moc01g33220.1 vs. NCBI nr
Match: XP_022146636.1 (gibberellin 2-beta-dioxygenase-like [Momordica charantia])

HSP 1 Score: 661.8 bits (1706), Expect = 3.1e-186
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLRTCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHTI 60
           MVVLSQPALELDHNSLLRTCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHTI
Sbjct: 1   MVVLSQPALELDHNSLLRTCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHTI 60

Query: 61  PMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLPI 120
           PMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLPI
Sbjct: 61  PMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLPI 120

Query: 121 PSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNHY 180
           PSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNHY
Sbjct: 121 PSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNHY 180

Query: 181 PPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFVN 240
           PPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFVN
Sbjct: 181 PPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFVN 240

Query: 241 VGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYKE 300
           VGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYKE
Sbjct: 241 VGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYKE 300

Query: 301 FTWWEYKTAAYKTRLADYRLGLFEK 326
           FTWWEYKTAAYKTRLADYRLGLFEK
Sbjct: 301 FTWWEYKTAAYKTRLADYRLGLFEK 325

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BLAST of Moc01g33220.1 vs. NCBI nr
Match: AUB30295.1 (gibberellin 2-beta-dioxygenase 8-like protein [Luffa aegyptiaca])

HSP 1 Score: 491.1 bits (1263), Expect = 7.3e-135
Identity = 245/327 (74.92%), Postives = 275/327 (84.10%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLR-TCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHT 60
           MVVLSQPAL LD  SLLR TC+ TAS  +IPEIDLS  +A+S IV+A EEFGF KL+NH 
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60

Query: 61  IPMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLP 120
           +P++ +TKLE+EALCFFKL QSEK+K+ PPDPLGYG KNIG NGD+GWIEY LL ANPLP
Sbjct: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120

Query: 121 IPSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNH 180
           I S N +S LAA  EYV AVKKL  E+ ELI EGL ++ RNA+S+LLNDEKSD CFRVNH
Sbjct: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180

Query: 181 YPPWPEEGAAMGA-RNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFF 240
           YPP P+  A  G+ RN++GFGEHTDP IISLLRSNNS GLQI LRDGAWV+VP D AA F
Sbjct: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240

Query: 241 VNVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLY 300
           VNVGD+LQVMSNGRF+SV HKVVA + +ERVSMIYF GPP SE+IAPLPEVLGDGEESLY
Sbjct: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTRLADYRLGLFEK 326
           KEFTWWEYKTAAYKTRLADYRLG FEK
Sbjct: 301 KEFTWWEYKTAAYKTRLADYRLGPFEK 327

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BLAST of Moc01g33220.1 vs. NCBI nr
Match: XP_022977872.1 (gibberellin 2-beta-dioxygenase [Cucurbita maxima])

HSP 1 Score: 475.7 bits (1223), Expect = 3.2e-130
Identity = 236/326 (72.39%), Postives = 271/326 (83.13%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLR-TCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHT 60
           MVVLSQPAL+L+  SLLR T +  AS+ADIPEIDLS  +AKS +VKA EEFGF KL+NH 
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  IPMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLP 120
           +P++ +TKLE E+LCFFKL QSEK+K+ PPDPLGYGSKNIG NGD+GWIEY LLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IPSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNH 180
           + S    S L+A  EYV AVKKL  E+VELITEGL ++ +NA+SRL+NDEK+D  FRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFV 240
           YPP PE  A  G RN++GFGEHTDP IISLLRSNNS GLQI  RDG WV+VP D  AFFV
Sbjct: 181 YPPCPEMQAVSG-RNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFV 240

Query: 241 NVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYK 300
           NVGDALQVM+NGRF+SV HKVVA ++KERVSMIYF GPP SE+I PL +VL +GEESLYK
Sbjct: 241 NVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYK 300

Query: 301 EFTWWEYKTAAYKTRLADYRLGLFEK 326
           EFTWWEYKTAA+KTRLADYRLG FEK
Sbjct: 301 EFTWWEYKTAAFKTRLADYRLGAFEK 325

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BLAST of Moc01g33220.1 vs. NCBI nr
Match: CBL59329.1 (Gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 475.7 bits (1223), Expect = 3.2e-130
Identity = 236/326 (72.39%), Postives = 271/326 (83.13%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLR-TCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHT 60
           MVVLSQPAL+L+  SLLR T +  AS+ADIPEIDLS  +AKS +VKA EEFGF KL+NH 
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  IPMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLP 120
           +P++ +TKLE E+LCFFKL QSEK+K+ PPDPLGYGSKNIG NGD+GWIEY LLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IPSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNH 180
           + S    S L+A  EYV AVKKL  E+VELITEGL ++ +NA+SRL+NDEK+D  FRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFV 240
           YPP PE  A  G RN++GFGEHTDP IISLLRSNNS GLQI  RDG WV+VP D  AFFV
Sbjct: 181 YPPCPEMQAVSG-RNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFV 240

Query: 241 NVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYK 300
           NVGDALQVM+NGRF+SV HKVVA ++KERVSMIYF GPP SE+I PL +VL +GEESLYK
Sbjct: 241 NVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYK 300

Query: 301 EFTWWEYKTAAYKTRLADYRLGLFEK 326
           EFTWWEYKTAA+KTRLADYRLG FEK
Sbjct: 301 EFTWWEYKTAAFKTRLADYRLGAFEK 325

[Close]

BLAST of Moc01g33220.1 vs. NCBI nr
Match: XP_038883200.1 (gibberellin 2-beta-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 474.9 bits (1221), Expect = 5.4e-130
Identity = 235/326 (72.09%), Postives = 271/326 (83.13%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLR-TCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHT 60
           MVVLSQP L+L+  SLLR TC+ TAS ADIPEIDLS  +AKS I+KA EEFGF KL+NH 
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60

Query: 61  IPMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLP 120
           +P++ +TKLE E+LCFFKL +SEK+K+ PP PLGYG KNIG NGD+GWIEY LLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IPSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNH 180
           I S N  S L A  EYV AVKKL SE+VELI EGL ++ RN +S+LLNDEK+D CFRVNH
Sbjct: 121 ILSQN--SFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNH 180

Query: 181 YPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFV 240
           YPP PE   ++G RN++GFGEHTDP IIS+LRSNNS+GLQI LRDGAWV+VP D  A FV
Sbjct: 181 YPPCPEM-QSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFV 240

Query: 241 NVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYK 300
           NVGDALQVM+NGRF+SV HKVVA   +ERVSMIYF GPP SE+I PLP++L +GEESLYK
Sbjct: 241 NVGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYK 300

Query: 301 EFTWWEYKTAAYKTRLADYRLGLFEK 326
           EFTWWEYKTAAYKT+LADYRLG FEK
Sbjct: 301 EFTWWEYKTAAYKTKLADYRLGAFEK 323

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BLAST of Moc01g33220.1 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 8.2e-105
Identity = 195/332 (58.73%), Postives = 238/332 (71.69%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLRTCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHTI 60
           MVVLSQPA  L+   LL+  ++T     IP +DL+H DAK+ IV A  +FGF KL+NH +
Sbjct: 1   MVVLSQPA--LNQFFLLKPFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHGV 60

Query: 61  PMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANP--- 120
           P++ +  LE EAL FFK  QSEK+++ PPDP GYGSK IGPNGD GW+EY LLN NP   
Sbjct: 61  PLELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVI 120

Query: 121 ----LPIPSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDS 180
               L I   N     A V  Y+ AVK +   ++EL+ EGL ++ RN +SRLL DEKSDS
Sbjct: 121 SPKSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDS 180

Query: 181 CFRVNHYPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPAD 240
           CFR+NHYPP PE  A    RNL+GFGEHTDP IIS+LRSN+++GLQI L DG WV+VP D
Sbjct: 181 CFRLNHYPPCPEVQAL--NRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPD 240

Query: 241 EAAFFVNVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDG 300
           + +FF+NVGDALQVM+NGRF+SV H+V+A   K R+SMIYF GP  SE IAPLP V+  G
Sbjct: 241 QTSFFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKG 300

Query: 301 EESLYKEFTWWEYKTAAYKTRLADYRLGLFEK 326
           EE LYKEFTW EYK AAY +RLAD RL  F+K
Sbjct: 301 EECLYKEFTWCEYKKAAYTSRLADNRLAPFQK 328

[Close]

BLAST of Moc01g33220.1 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 3.3e-98
Identity = 180/334 (53.89%), Postives = 245/334 (73.35%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLRTCR--ATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINH 60
           MVVL QP    +H SL+ T +     +S  IP ++L+  +AK+RIVKA EEFGF K++NH
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNH 60

Query: 61  TIPMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANP- 120
            +  + +T+LEQEA+ FF LPQS K ++ PP+P GYG+K IGPNGD GWIEY LLNANP 
Sbjct: 61  GVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQ 120

Query: 121 LPIPSGN------HQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKS 180
           L  P  +       Q    +V EY+  +K++  +++E++ E L ++ R+ +S++L DEKS
Sbjct: 121 LSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKS 180

Query: 181 DSCFRVNHYPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVP 240
           DSC R+NHYP   EE   M     +GFGEHTDP IIS+LRSNN+AGLQI ++DG+WVAVP
Sbjct: 181 DSCLRLNHYPAAEEEAEKMVK---VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 ADEAAFFVNVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLG 300
            D ++FF+NVGDALQVM+NGRF+SV H+V+A   + R+SMIYF GPP S++IAPLP ++ 
Sbjct: 241 PDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVP 300

Query: 301 DGEESLYKEFTWWEYKTAAYKTRLADYRLGLFEK 326
           + ++ LYKEFTW +YK++AYK++L DYRLGLFEK
Sbjct: 301 EQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of Moc01g33220.1 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.3e-94
Identity = 174/328 (53.05%), Postives = 235/328 (71.65%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLRTCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHTI 60
           MV+LS+P  E    + +R       S+ IP +DLS  DAK+ IVKA E+FGF K+INH I
Sbjct: 1   MVLLSKPTSE--QYTYVRNNMPITFSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHGI 60

Query: 61  PMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANP--- 120
           P+D+I++LE EA  FF LPQ+EKEK+ P +P GYG+K IG NGD GWIEY LL  N    
Sbjct: 61  PLDAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTNQDHN 120

Query: 121 LPIPSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRV 180
             +   +       + +Y  A++ +  EI++L+ EGL ++ +N  S+L+ D++SD  FRV
Sbjct: 121 FSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFRV 180

Query: 181 NHYPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAF 240
           NHYP  PE   A+   NL+GFGEHTDP IIS+LRSNN++G QI LRDG+W++VP D ++F
Sbjct: 181 NHYPACPE--LAINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSF 240

Query: 241 FVNVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESL 300
           F+NVGD+LQVM+NGRF+SV H+V+A     R+SMIYF GPP SE+IAPLP ++  G+ESL
Sbjct: 241 FINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPSLM-KGKESL 300

Query: 301 YKEFTWWEYKTAAYKTRLADYRLGLFEK 326
           YKEFTW+EYK++ Y +RLAD RLG +E+
Sbjct: 301 YKEFTWFEYKSSTYGSRLADNRLGNYER 323

[Close]

BLAST of Moc01g33220.1 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 3.1e-88
Identity = 171/333 (51.35%), Postives = 228/333 (68.47%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLRTCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHTI 60
           MV++ QPA   D N L    +       IP IDL+ +DAK++IVKA EEFGF K+INH +
Sbjct: 1   MVIVLQPA-SFDSN-LYVNPKCKPRPVLIPVIDLTDSDAKTQIVKACEEFGFFKVINHGV 60

Query: 61  PMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLPI 120
             D +T+LEQEA+ FF L  S K+K+ PPDP GYG+K IGPNGD GW+EY LLNAN L +
Sbjct: 61  RPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNAN-LCL 120

Query: 121 PSGNHQSLL--------AAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSD 180
            S    ++          AV EY+  +K++ S+ +E++ E L ++ +  +SRL+  ++SD
Sbjct: 121 ESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESD 180

Query: 181 SCFRVNHYPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPA 240
           SC R+NHYP    E      +  +GFGEHTDP +ISLLRSN++ GLQI ++DG WV V  
Sbjct: 181 SCLRMNHYP----EKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTP 240

Query: 241 DEAAFFVNVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGD 300
           D ++FFV VGD LQVM+NGRF+SV H+VV    + R+SMIYFAGPP SE+IAPL  ++  
Sbjct: 241 DHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPK 300

Query: 301 GEESLYKEFTWWEYKTAAYKTRLADYRLGLFEK 326
            ++ LY EFTW +YK +AYKT+L DYRLGLFEK
Sbjct: 301 QDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEK 326

[Close]

BLAST of Moc01g33220.1 vs. ExPASy Swiss-Prot
Match: Q8S0S6 (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.3e-86
Identity = 169/332 (50.90%), Postives = 224/332 (67.47%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLRTCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHTI 60
           MVVL+ P   +DH  LLR+       + +P +DL    A   +V A E +GF K++NH +
Sbjct: 1   MVVLAGPP-AVDHIPLLRSPDPGDVFSGVPVVDLGSPGAARAVVDACERYGFFKVVNHGV 60

Query: 61  PMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLN------ 120
             D++ K E EA+ FF   Q +K++S P  P GYGSK IG NGD GW+EY LL       
Sbjct: 61  ATDTMDKAESEAVRFFSQTQPDKDRSGPAYPFGYGSKRIGFNGDMGWLEYLLLALDDASL 120

Query: 121 ANPLPIPSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSC 180
           A+   +PS       AA+NEY+  V+K+   ++E ++EGL +   +A+S L+  E SD  
Sbjct: 121 ADACTVPS--CAVFRAALNEYISGVRKVAVRVMEAMSEGLGIAQADALSALVTAEGSDQV 180

Query: 181 FRVNHYPPWPEEGAAMG-ARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPAD 240
           FRVNHYPP     A  G   ++ GFGEHTDP ++S+LRSN ++GLQI LRDG WV+VP+D
Sbjct: 181 FRVNHYPPC---RALQGLGCSVTGFGEHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSD 240

Query: 241 EAAFFVNVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDG 300
             +FFVNVGD+LQV++NGRF+SV H+VVA + K RVS IYF GPP ++RIAPLP++LG+G
Sbjct: 241 RDSFFVNVGDSLQVLTNGRFKSVKHRVVANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEG 300

Query: 301 EESLYKEFTWWEYKTAAYKTRLADYRLGLFEK 326
           E+SLYKEFTW EYK AAYK+RL D RL  FEK
Sbjct: 301 EQSLYKEFTWDEYKKAAYKSRLGDNRLAQFEK 326

[Close]

BLAST of Moc01g33220.1 vs. ExPASy TrEMBL
Match: A0A6J1CZY6 (gibberellin 2-beta-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111015790 PE=3 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 1.5e-186
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLRTCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHTI 60
           MVVLSQPALELDHNSLLRTCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHTI
Sbjct: 1   MVVLSQPALELDHNSLLRTCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHTI 60

Query: 61  PMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLPI 120
           PMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLPI
Sbjct: 61  PMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLPI 120

Query: 121 PSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNHY 180
           PSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNHY
Sbjct: 121 PSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNHY 180

Query: 181 PPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFVN 240
           PPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFVN
Sbjct: 181 PPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFVN 240

Query: 241 VGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYKE 300
           VGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYKE
Sbjct: 241 VGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYKE 300

Query: 301 FTWWEYKTAAYKTRLADYRLGLFEK 326
           FTWWEYKTAAYKTRLADYRLGLFEK
Sbjct: 301 FTWWEYKTAAYKTRLADYRLGLFEK 325

[Close]

BLAST of Moc01g33220.1 vs. ExPASy TrEMBL
Match: A0A2H4V3T7 (Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.5e-135
Identity = 245/327 (74.92%), Postives = 275/327 (84.10%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLR-TCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHT 60
           MVVLSQPAL LD  SLLR TC+ TAS  +IPEIDLS  +A+S IV+A EEFGF KL+NH 
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60

Query: 61  IPMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLP 120
           +P++ +TKLE+EALCFFKL QSEK+K+ PPDPLGYG KNIG NGD+GWIEY LL ANPLP
Sbjct: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120

Query: 121 IPSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNH 180
           I S N +S LAA  EYV AVKKL  E+ ELI EGL ++ RNA+S+LLNDEKSD CFRVNH
Sbjct: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180

Query: 181 YPPWPEEGAAMGA-RNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFF 240
           YPP P+  A  G+ RN++GFGEHTDP IISLLRSNNS GLQI LRDGAWV+VP D AA F
Sbjct: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240

Query: 241 VNVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLY 300
           VNVGD+LQVMSNGRF+SV HKVVA + +ERVSMIYF GPP SE+IAPLPEVLGDGEESLY
Sbjct: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTRLADYRLGLFEK 326
           KEFTWWEYKTAAYKTRLADYRLG FEK
Sbjct: 301 KEFTWWEYKTAAYKTRLADYRLGPFEK 327

[Close]

BLAST of Moc01g33220.1 vs. ExPASy TrEMBL
Match: A0A6J1IJN1 (gibberellin 2-beta-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111478033 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.5e-130
Identity = 236/326 (72.39%), Postives = 271/326 (83.13%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLR-TCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHT 60
           MVVLSQPAL+L+  SLLR T +  AS+ADIPEIDLS  +AKS +VKA EEFGF KL+NH 
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  IPMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLP 120
           +P++ +TKLE E+LCFFKL QSEK+K+ PPDPLGYGSKNIG NGD+GWIEY LLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IPSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNH 180
           + S    S L+A  EYV AVKKL  E+VELITEGL ++ +NA+SRL+NDEK+D  FRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFV 240
           YPP PE  A  G RN++GFGEHTDP IISLLRSNNS GLQI  RDG WV+VP D  AFFV
Sbjct: 181 YPPCPEMQAVSG-RNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFV 240

Query: 241 NVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYK 300
           NVGDALQVM+NGRF+SV HKVVA ++KERVSMIYF GPP SE+I PL +VL +GEESLYK
Sbjct: 241 NVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYK 300

Query: 301 EFTWWEYKTAAYKTRLADYRLGLFEK 326
           EFTWWEYKTAA+KTRLADYRLG FEK
Sbjct: 301 EFTWWEYKTAAFKTRLADYRLGAFEK 325

[Close]

BLAST of Moc01g33220.1 vs. ExPASy TrEMBL
Match: I0J1J0 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.5e-130
Identity = 236/326 (72.39%), Postives = 271/326 (83.13%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLR-TCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHT 60
           MVVLSQPAL+L+  SLLR T +  AS+ADIPEIDLS  +AKS +VKA EEFGF KL+NH 
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  IPMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLP 120
           +P++ +TKLE E+LCFFKL QSEK+K+ PPDPLGYGSKNIG NGD+GWIEY LLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IPSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNH 180
           + S    S L+A  EYV AVKKL  E+VELITEGL ++ +NA+SRL+NDEK+D  FRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFV 240
           YPP PE  A  G RN++GFGEHTDP IISLLRSNNS GLQI  RDG WV+VP D  AFFV
Sbjct: 181 YPPCPEMQAVSG-RNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFV 240

Query: 241 NVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYK 300
           NVGDALQVM+NGRF+SV HKVVA ++KERVSMIYF GPP SE+I PL +VL +GEESLYK
Sbjct: 241 NVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYK 300

Query: 301 EFTWWEYKTAAYKTRLADYRLGLFEK 326
           EFTWWEYKTAA+KTRLADYRLG FEK
Sbjct: 301 EFTWWEYKTAAFKTRLADYRLGAFEK 325

[Close]

BLAST of Moc01g33220.1 vs. ExPASy TrEMBL
Match: A0A1S3AZ81 (gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103484366 PE=3 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 3.2e-128
Identity = 233/326 (71.47%), Postives = 265/326 (81.29%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLR-TCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHT 60
           MVVLSQP L+L+  SLLR TC+ TAS ADIPEIDLS  +AK  IVKA EEFGF KL+NH 
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  IPMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLP 120
           +P++ +TKLE E+LCFFKL +SEK+K+ PP PLGYGSKNIG NGD+GWIEY L+NANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 IPSGNHQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNH 180
           I S      L A  EYV AVKKL  E+VELI EGL ++ RNA+S+LLNDEK+D CFRVNH
Sbjct: 121 IFS---HDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFV 240
           YPP PE     G RN++GFGEHTDP IIS+LRSNNS GLQI LRDGAWV+VP D  A FV
Sbjct: 181 YPPCPEMQGLSG-RNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALFV 240

Query: 241 NVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYK 300
           NVGDALQVM+NGRF+SV HKVV    +ERVSMIYF GPP SE+I PLPE+L DGEESLYK
Sbjct: 241 NVGDALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLYK 300

Query: 301 EFTWWEYKTAAYKTRLADYRLGLFEK 326
           EFTWWEYKTAAYK++LADYRLG FEK
Sbjct: 301 EFTWWEYKTAAYKSKLADYRLGAFEK 322

[Close]

BLAST of Moc01g33220.1 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 359.8 bits (922), Expect = 2.4e-99
Identity = 180/334 (53.89%), Postives = 245/334 (73.35%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLRTCR--ATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINH 60
           MVVL QP    +H SL+ T +     +S  IP ++L+  +AK+RIVKA EEFGF K++NH
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNH 60

Query: 61  TIPMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANP- 120
            +  + +T+LEQEA+ FF LPQS K ++ PP+P GYG+K IGPNGD GWIEY LLNANP 
Sbjct: 61  GVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQ 120

Query: 121 LPIPSGN------HQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKS 180
           L  P  +       Q    +V EY+  +K++  +++E++ E L ++ R+ +S++L DEKS
Sbjct: 121 LSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKS 180

Query: 181 DSCFRVNHYPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVP 240
           DSC R+NHYP   EE   M     +GFGEHTDP IIS+LRSNN+AGLQI ++DG+WVAVP
Sbjct: 181 DSCLRLNHYPAAEEEAEKMVK---VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 ADEAAFFVNVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLG 300
            D ++FF+NVGDALQVM+NGRF+SV H+V+A   + R+SMIYF GPP S++IAPLP ++ 
Sbjct: 241 PDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVP 300

Query: 301 DGEESLYKEFTWWEYKTAAYKTRLADYRLGLFEK 326
           + ++ LYKEFTW +YK++AYK++L DYRLGLFEK
Sbjct: 301 EQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of Moc01g33220.1 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 326.6 bits (836), Expect = 2.2e-89
Identity = 171/333 (51.35%), Postives = 228/333 (68.47%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLRTCRATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINHTI 60
           MV++ QPA   D N L    +       IP IDL+ +DAK++IVKA EEFGF K+INH +
Sbjct: 1   MVIVLQPA-SFDSN-LYVNPKCKPRPVLIPVIDLTDSDAKTQIVKACEEFGFFKVINHGV 60

Query: 61  PMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLPI 120
             D +T+LEQEA+ FF L  S K+K+ PPDP GYG+K IGPNGD GW+EY LLNAN L +
Sbjct: 61  RPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNAN-LCL 120

Query: 121 PSGNHQSLL--------AAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSD 180
            S    ++          AV EY+  +K++ S+ +E++ E L ++ +  +SRL+  ++SD
Sbjct: 121 ESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESD 180

Query: 181 SCFRVNHYPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPA 240
           SC R+NHYP    E      +  +GFGEHTDP +ISLLRSN++ GLQI ++DG WV V  
Sbjct: 181 SCLRMNHYP----EKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTP 240

Query: 241 DEAAFFVNVGDALQVMSNGRFRSVVHKVVAGAAKERVSMIYFAGPPPSERIAPLPEVLGD 300
           D ++FFV VGD LQVM+NGRF+SV H+VV    + R+SMIYFAGPP SE+IAPL  ++  
Sbjct: 241 DHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPK 300

Query: 301 GEESLYKEFTWWEYKTAAYKTRLADYRLGLFEK 326
            ++ LY EFTW +YK +AYKT+L DYRLGLFEK
Sbjct: 301 QDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEK 326

[Close]

BLAST of Moc01g33220.1 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 312.0 bits (798), Expect = 5.7e-85
Identity = 154/305 (50.49%), Postives = 215/305 (70.49%), Query Frame = 0


Query: 29  IPEIDLSHADAKSRIVKAFEEFGFCKLINHTIPMDSITKLEQEALCFFKLPQSEKEKSLP 88
           IP ID+S  ++K  +VKA E+FGF K+INH +  + ++ LE E + FF LP+SEK + + 
Sbjct: 18  IPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ-VA 77

Query: 89  PDPLGYGSKNIGPNGDEGWIEYFLLNAN------PL-PIPSGNHQSLLAAVNEYVPAVKK 148
             P GYG+  IG NGD GW+EY L+NAN      PL P    +  +   A+ EY  +V+K
Sbjct: 78  GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTSVRK 137

Query: 149 LGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNHYPPWP-EEGAAMGARNLLGFGE 208
           +  +++E IT+GL +K RN +S+L++D+ +DS  R+NHYPP P       G +N++GFGE
Sbjct: 138 MTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIGFGE 197

Query: 209 HTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFVNVGDALQVMSNGRFRSVVHKV 268
           HTDP IIS+LRSNN++GLQI L DG+W++VP D  +FF NVGD+LQVM+NGRF+SV H+V
Sbjct: 198 HTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVRHRV 257

Query: 269 VAGAAKERVSMIYFAGPPPSERIAPLPEVLGDGEESLYKEFTWWEYKTAAYKTRLADYRL 326
           +A   K RVSMIYFAGP  ++RIAPL  ++ + +E LY+EFTW EYK + Y +RL+D RL
Sbjct: 258 LANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSDNRL 317

[Close]

BLAST of Moc01g33220.1 vs. TAIR 10
Match: AT1G30040.2 (gibberellin 2-oxidase )

HSP 1 Score: 257.7 bits (657), Expect = 1.3e-68
Identity = 133/256 (51.95%), Postives = 180/256 (70.31%), Query Frame = 0


Query: 1   MVVLSQPALELDHNSLLRTCR--ATASSADIPEIDLSHADAKSRIVKAFEEFGFCKLINH 60
           MVVL QP    +H SL+ T +     +S  IP ++L+  +AK+RIVKA EEFGF K++NH
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNH 60

Query: 61  TIPMDSITKLEQEALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANP- 120
            +  + +T+LEQEA+ FF LPQS K ++ PP+P GYG+K IGPNGD GWIEY LLNANP 
Sbjct: 61  GVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQ 120

Query: 121 LPIPSGN------HQSLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKS 180
           L  P  +       Q    +V EY+  +K++  +++E++ E L ++ R+ +S++L DEKS
Sbjct: 121 LSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKS 180

Query: 181 DSCFRVNHYPPWPEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVP 240
           DSC R+NHYP   EE   M     +GFGEHTDP IIS+LRSNN+AGLQI ++DG+WVAVP
Sbjct: 181 DSCLRLNHYPAAEEEAEKMVK---VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 ADEAAFFVNVGDALQV 248
            D ++FF+NVGDALQV
Sbjct: 241 PDHSSFFINVGDALQV 253

[Close]

BLAST of Moc01g33220.1 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 247.3 bits (630), Expect = 1.7e-65
Identity = 138/322 (42.86%), Postives = 190/322 (59.01%), Query Frame = 0


Query: 16  LLRTCRATASSAD--IPEIDLSHAD---AKSRIVKAFEEFGFCKLINHTIPMDSITKLEQ 75
           L  T + T SS +   P ID S  D      +IVKA E  GF K+INH +  + I + E 
Sbjct: 9   LQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEH 68

Query: 76  EALCFFKLPQSEKEKSLPPDPLGYGSKNIGPNGDEGWIEYFLLNANPLPIPSGNHQ---- 135
           E   FF  P+S+K ++ P  P GYG KNIG NGD G +EY LL+ANP  +   +      
Sbjct: 69  EGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHD 128

Query: 136 ---SLLAAVNEYVPAVKKLGSEIVELITEGLNVKARNAVSRLLNDEKSDSCFRVNHYPPW 195
                 +A N+Y+  V+ L  EI++L  E L  +  + VS L+ D +SDS  R+NHYPP 
Sbjct: 129 DPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPA 188

Query: 196 PEEGAAMGARNLLGFGEHTDPHIISLLRSNNSAGLQIRLRDGAWVAVPADEAAFFVNVGD 255
           P   + +G    +GFGEH+DP I+++LRSN+  GL+I  RDG W+ +P+D   FFV VGD
Sbjct: 189 PYALSGVGQ---IGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGD 248

Query: 256 ALQVMSNGRFRSVVHKVVAGAAKE-RVSMIYFAGPPPSERIAPLPEVLGDGEESLYKEFT 315
            LQ ++NGRF SV H+V+A  AK+ R+S +YFA PP   +I+PLP+++       Y  FT
Sbjct: 249 CLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFT 308

Query: 316 WWEYKTAAYKTRLADYRLGLFE 325
           W +YK A Y  RL   RL  F+
Sbjct: 309 WGDYKKATYSLRLDVPRLEFFK 327

The following BLAST results are available for this feature:

BLAST of Moc01g33220.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146636.1	3.1e-186	100.00	gibberellin 2-beta-dioxygenase-like [Momordica charantia]	[more]
AUB30295.1	7.3e-135	74.92	gibberellin 2-beta-dioxygenase 8-like protein [Luffa aegyptiaca]	[more]
XP_022977872.1	3.2e-130	72.39	gibberellin 2-beta-dioxygenase [Cucurbita maxima]	[more]
CBL59329.1	3.2e-130	72.39	Gibberellin 2-oxidase [Cucurbita maxima]	[more]
XP_038883200.1	5.4e-130	72.09	gibberellin 2-beta-dioxygenase-like [Benincasa hispida]	[more]

BLAST of Moc01g33220.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XG83	8.2e-105	58.73	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
Q9XFR9	3.3e-98	53.89	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q9SQ80	1.3e-94	53.05	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
O64692	3.1e-88	51.35	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ...	[more]
Q8S0S6	1.3e-86	50.90	Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]

BLAST of Moc01g33220.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CZY6	1.5e-186	100.00	gibberellin 2-beta-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC1110157...	[more]
A0A2H4V3T7	3.5e-135	74.92	Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 S...	[more]
A0A6J1IJN1	1.5e-130	72.39	gibberellin 2-beta-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111478033 PE=3 ...	[more]
I0J1J0	1.5e-130	72.39	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1	[more]
A0A1S3AZ81	3.2e-128	71.47	gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103484366 PE=3...	[more]

BLAST of Moc01g33220.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30040.1	2.4e-99	53.89	gibberellin 2-oxidase	[more]
AT2G34555.1	2.2e-89	51.35	gibberellin 2-oxidase 3	[more]
AT1G78440.1	5.7e-85	50.49	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT1G30040.2	1.3e-68	51.95	gibberellin 2-oxidase	[more]
AT1G02400.1	1.7e-65	42.86	gibberellin 2-oxidase 6	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 5..317 e-value: 8.3E-73 score: 247.5
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 172..276 e-value: 1.1E-22 score: 80.4
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 29..116 e-value: 5.0E-11 score: 43.4
None	No IPR available	PANTHER	PTHR47990:SF121	GIBBERELLIN 2-BETA-DIOXYGENASE 2	coord: 10..324
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 10..324
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 9..317
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 170..277 score: 12.040836

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc01g33220	Moc01g33220	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc01g33220.1.exon1	Moc01g33220.1.exon1	exon
Moc01g33220.1.exon2	Moc01g33220.1.exon2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc01g33220.1	cds.Moc01g33220.1	CDS
cds.Moc01g33220.1	cds.Moc01g33220.1_2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc01g33220.1	Moc01g33220.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding