Moc01g17290.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g17290.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Calcium-binding EF-hand family protein
Location	chr1: 11657148 .. 11657747 (-)
Sequence length	600
RNA-Seq Expression	Moc01g17290.1
Synteny	Moc01g17290.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAATCCCCAAGCGATTTAGCCCTAAACGCCTCTTCCGATCCAAGAAGAATCGCGCCGACGTCTCCCGCCGCTCCGATCCGCCGTCGTTCGGCTCCGCTACCTCCTCTTCTTCCTCCTCTTCCGAGAACTTCTTCAAGCCTCAAACCGCTGCGAGTGCAGGCGGCTACGGAACTCCCACCAGCGTCTTGCCCGATTCCGCCGCCGACTGGTCCGAAAACAACGGCGTTGTGTCCAGGAAGGAGCTGGAGGCTATTCTCCACCGGATCGGAGCGGATCCGCCGTCGGAGGAGGAGGTGGCGATGATGCTCGGTGAGTTCGACGGCGACGGTTTCATACGCCTCGAGGCGCTGATGGACCGGGTCGGGTCGGGGTTCGGCCCGGCCTGCGAGGCGGAGCTCCGGGAGGCGTTTGAGTTCTTCGACGCCGACCACGACGGGAGGATCACGGCGGAGGAGCTCCACGGGGTGTTCAGTTCCATCGGAGACGAGCGGTGCACGTTAGAGGACTGCCGGCGCATGATAGCGGATGTGGATGAGAACGGGGACGGGTTCGTGTGCTTCTCGGAGTTTGTGCGTATGATGGAGCTGCAGGGATGA

mRNA sequence

ATGAAAATCCCCAAGCGATTTAGCCCTAAACGCCTCTTCCGATCCAAGAAGAATCGCGCCGACGTCTCCCGCCGCTCCGATCCGCCGTCGTTCGGCTCCGCTACCTCCTCTTCTTCCTCCTCTTCCGAGAACTTCTTCAAGCCTCAAACCGCTGCGAGTGCAGGCGGCTACGGAACTCCCACCAGCGTCTTGCCCGATTCCGCCGCCGACTGGTCCGAAAACAACGGCGTTGTGTCCAGGAAGGAGCTGGAGGCTATTCTCCACCGGATCGGAGCGGATCCGCCGTCGGAGGAGGAGGTGGCGATGATGCTCGGTGAGTTCGACGGCGACGGTTTCATACGCCTCGAGGCGCTGATGGACCGGGTCGGGTCGGGGTTCGGCCCGGCCTGCGAGGCGGAGCTCCGGGAGGCGTTTGAGTTCTTCGACGCCGACCACGACGGGAGGATCACGGCGGAGGAGCTCCACGGGGTGTTCAGTTCCATCGGAGACGAGCGGTGCACGTTAGAGGACTGCCGGCGCATGATAGCGGATGTGGATGAGAACGGGGACGGGTTCGTGTGCTTCTCGGAGTTTGTGCGTATGATGGAGCTGCAGGGATGA

Coding sequence (CDS)

ATGAAAATCCCCAAGCGATTTAGCCCTAAACGCCTCTTCCGATCCAAGAAGAATCGCGCCGACGTCTCCCGCCGCTCCGATCCGCCGTCGTTCGGCTCCGCTACCTCCTCTTCTTCCTCCTCTTCCGAGAACTTCTTCAAGCCTCAAACCGCTGCGAGTGCAGGCGGCTACGGAACTCCCACCAGCGTCTTGCCCGATTCCGCCGCCGACTGGTCCGAAAACAACGGCGTTGTGTCCAGGAAGGAGCTGGAGGCTATTCTCCACCGGATCGGAGCGGATCCGCCGTCGGAGGAGGAGGTGGCGATGATGCTCGGTGAGTTCGACGGCGACGGTTTCATACGCCTCGAGGCGCTGATGGACCGGGTCGGGTCGGGGTTCGGCCCGGCCTGCGAGGCGGAGCTCCGGGAGGCGTTTGAGTTCTTCGACGCCGACCACGACGGGAGGATCACGGCGGAGGAGCTCCACGGGGTGTTCAGTTCCATCGGAGACGAGCGGTGCACGTTAGAGGACTGCCGGCGCATGATAGCGGATGTGGATGAGAACGGGGACGGGTTCGTGTGCTTCTCGGAGTTTGTGCGTATGATGGAGCTGCAGGGATGA

Protein sequence

MKIPKRFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTPTSVLPDSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFIRLEALMDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADVDENGDGFVCFSEFVRMMELQG

Homology

[Close]

BLAST of Moc01g17290.1 vs. NCBI nr
Match: XP_022153870.1 (probable calcium-binding protein CML36 [Momordica charantia])

HSP 1 Score: 392.9 bits (1008), Expect = 1.7e-105
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTP 60
           MKIPKRFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTP
Sbjct: 1   MKIPKRFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTP 60

Query: 61  TSVLPDSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFIRLEALMD 120
           TSVLPDSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFIRLEALMD
Sbjct: 61  TSVLPDSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFIRLEALMD 120

Query: 121 RVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADVDE 180
           RVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADVDE
Sbjct: 121 RVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADVDE 180

Query: 181 NGDGFVCFSEFVRMMELQG 200
           NGDGFVCFSEFVRMMELQG
Sbjct: 181 NGDGFVCFSEFVRMMELQG 199

[Close]

BLAST of Moc01g17290.1 vs. NCBI nr
Match: XP_023543601.1 (probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 280.4 bits (716), Expect = 1.2e-71
Identity = 159/201 (79.10%), Postives = 167/201 (83.08%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKN-RADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGT 60
           MKIPKRFSPKRLFRSKKN  ADVS RSDPPSFGS TSSSSSSSENFFKP TA    GYGT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVS-RSDPPSFGSGTSSSSSSSENFFKPHTA----GYGT 60

Query: 61  PTSVL--PDSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFIRLEA 120
           PTSVL    S +D  EN+GVVSRKELEAIL RI     SEEEVAMML E DG GFIRL+ 
Sbjct: 61  PTSVLLAGCSDSDQRENDGVVSRKELEAILSRIA----SEEEVAMMLSEVDGGGFIRLDE 120

Query: 121 LMDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIAD 180
           LM RVGSGF    E+ELRE FEFFDADHDGRI+AEELHGV+ SIGDERCTLEDCRRMI D
Sbjct: 121 LMARVGSGFDSVAESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRD 180

Query: 181 VDENGDGFVCFSEFVRMMELQ 199
           VDE+GDGFVCF+EFVRMMELQ
Sbjct: 181 VDEDGDGFVCFTEFVRMMELQ 192

[Close]

BLAST of Moc01g17290.1 vs. NCBI nr
Match: XP_022932888.1 (probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 278.5 bits (711), Expect = 4.6e-71
Identity = 155/200 (77.50%), Postives = 164/200 (82.00%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTP 60
           MKIPKRFSPKRLFRSKKN +    RSDPPSFGS TSSSSSSSENFFKP TA    GYGTP
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTA----GYGTP 60

Query: 61  TSVL--PDSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFIRLEAL 120
           TSVL    S +D  EN+GVVSRKELEAIL RI     SEEEVAMML E DG GFIRL+ L
Sbjct: 61  TSVLLAGCSDSDQRENDGVVSRKELEAILSRIA----SEEEVAMMLSEVDGGGFIRLDEL 120

Query: 121 MDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADV 180
           M RVGSGF    E+ELRE FEFFDADHDGRI+AEELHGV+ SIGDERCTLEDCRRMI DV
Sbjct: 121 MARVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDV 180

Query: 181 DENGDGFVCFSEFVRMMELQ 199
           DE+GDGFVCF+EFVRMMELQ
Sbjct: 181 DEDGDGFVCFTEFVRMMELQ 192

[Close]

BLAST of Moc01g17290.1 vs. NCBI nr
Match: XP_022967907.1 (probable calcium-binding protein CML36 [Cucurbita maxima])

HSP 1 Score: 274.6 bits (701), Expect = 6.6e-70
Identity = 157/200 (78.50%), Postives = 165/200 (82.50%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKN-RADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGT 60
           MKIPKRFSPKRLFRSKKN  ADVS RSDPPSFGS TSSSSSSSENFFKP TA    GYGT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVS-RSDPPSFGSGTSSSSSSSENFFKPHTA----GYGT 60

Query: 61  PTSVLPD-SAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFIRLEAL 120
           PTSVL   S +D  EN+ VVSRKELEAIL RI     SEEEVAMML E D  GFIRL+ L
Sbjct: 61  PTSVLAGCSDSDQRENDVVVSRKELEAILSRIA----SEEEVAMMLSEVDDGGFIRLDEL 120

Query: 121 MDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADV 180
           M RVGSGF    E+ELRE FEFFDADHDGRI+AEELHGV+ SIGDERCTLEDCRRMI DV
Sbjct: 121 MARVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDV 180

Query: 181 DENGDGFVCFSEFVRMMELQ 199
           DE+GDGFVCF+EFVRMMELQ
Sbjct: 181 DEDGDGFVCFTEFVRMMELQ 191

[Close]

BLAST of Moc01g17290.1 vs. NCBI nr
Match: XP_008457589.1 (PREDICTED: probable calcium-binding protein CML36 [Cucumis melo] >KAA0062715.1 putative calcium-binding protein CML36 [Cucumis melo var. makuwa])

HSP 1 Score: 266.5 bits (680), Expect = 1.8e-67
Identity = 157/201 (78.11%), Postives = 166/201 (82.59%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNR-ADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGT 60
           MKIPKRFSPKRLFRSKK++ ADVS RSDPPSFGS TSSSSSSSENFFKP +  +  GYGT
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVS-RSDPPSFGSGTSSSSSSSENFFKPNSVNT--GYGT 61

Query: 61  PTSVLPDSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFD--GDGFIRLEA 120
           PTSVL    +D  EN GVV+RKELEAIL RI     SEEEVAMML E D  GDGFIRLE 
Sbjct: 62  PTSVLD---SDVRENEGVVTRKELEAILSRIA----SEEEVAMMLSEVDSGGDGFIRLEE 121

Query: 121 LMDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIAD 180
           LM RVGSG   A E+ELRE FEFFDAD DGRITAEELHGVF SIGDE+CTLEDCRRMI D
Sbjct: 122 LMARVGSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRD 181

Query: 181 VDENGDGFVCFSEFVRMMELQ 199
           VDENGDGFVCF+EFVRMMELQ
Sbjct: 182 VDENGDGFVCFAEFVRMMELQ 191

[Close]

BLAST of Moc01g17290.1 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 3.8e-44
Identity = 110/213 (51.64%), Postives = 138/213 (64.79%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTP 60
           MK+ K   PKR F   K+R+ VS+     SFGSA+SSS    +N          GG  TP
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKN----SGGDGGGGSVTP 60

Query: 61  TSVLPDSAADWS-------------ENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEF 120
           TS+LP+  + +S             +N+G VSR +LE++L R+G DP +EEE+ +ML E 
Sbjct: 61  TSILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEV 120

Query: 121 --DGDGFIRLEALMDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDER 180
             DGDG IRLE L  RV S        EL+E FEFFDAD DG I+A+EL  VFS+IGDER
Sbjct: 121 DCDGDGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDER 180

Query: 181 CTLEDCRRMIADVDENGDGFVCFSEFVRMMELQ 199
           CTL+DC+RMIADVDE+GDGFVCF+EF RMM+LQ
Sbjct: 181 CTLDDCKRMIADVDEDGDGFVCFTEFSRMMDLQ 208

[Close]

BLAST of Moc01g17290.1 vs. ExPASy Swiss-Prot
Match: P30188 (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 166.4 bits (420), Expect = 3.3e-40
Identity = 107/206 (51.94%), Postives = 130/206 (63.11%), Query Frame = 0


Query: 6   RFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTPTSVLP 65
           R SPKRLFR+ K++A VS RS+P SF S  SSSSS        Q     G   TP SVLP
Sbjct: 9   RLSPKRLFRT-KSKASVS-RSEPSSFSSNASSSSSDGSYGNLKQ-----GPTATPISVLP 68

Query: 66  DSAADW------------SENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDG--DG 125
            ++ D+             +++GVVSR +L A++ R+  +PPS+EEV++ML E DG   G
Sbjct: 69  QNSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGG 128

Query: 126 FIRLEALMDRVG--SGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLE 185
            I LE L  RV   SG G     ELRE FE FD D +G+I+AEELH VF  IGDERCTLE
Sbjct: 129 CISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLE 188

Query: 186 DCRRMIADVDENGDGFVCFSEFVRMM 196
           +C RMIA VD NGDGFVCF +F RMM
Sbjct: 189 ECMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Moc01g17290.1 vs. ExPASy Swiss-Prot
Match: Q5ZCK5 (Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 GN=CML16 PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.2e-19
Identity = 64/131 (48.85%), Postives = 77/131 (58.78%), Query Frame = 0


Query: 72  SENNGVVSRKELEAILHRIGADPPSE----EEVAMMLGEF--DGDGFIRLEALMDRVGSG 131
           ++ +G +S  EL A+   I A PPSE     EVA M+ E   D DGF+ L       G G
Sbjct: 37  ADGDGRISPSELAAVTRAI-APPPSESAGGREVAAMMNELDTDRDGFVDLGEFAAFHGRG 96

Query: 132 FGPA-CEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADVDENGDG 191
            G A  EAELR AF+ +D D DGRITA EL  V   IG E C+ E+C RMIA VD +GDG
Sbjct: 97  RGDAEHEAELRAAFDVYDVDGDGRITAAELGKVLGRIG-EGCSAEECERMIASVDVDGDG 156

Query: 192 FVCFSEFVRMM 196
            V F EF +MM
Sbjct: 157 CVGFEEFKKMM 165

[Close]

BLAST of Moc01g17290.1 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 5.5e-19
Identity = 63/154 (40.91%), Postives = 87/154 (56.49%), Query Frame = 0


Query: 52  ASAGGYGTPTSVLPDSAADW------SENNGVVSRKELEAILHRIGADPPSEEEVAMMLG 111
           AS GG   PT   P+   +       +  +G +SR EL A+   +G    +++E+A M+ 
Sbjct: 27  ASGGGSPAPT---PEEEMERVFRKFDANGDGRISRSELGALFESLG-HAATDDELARMMA 86

Query: 112 E--FDGDGFIRLE--ALMDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSI 171
           E   DGDGFI L+  A ++   SG   A E +LR AF  FDAD +G I+A EL  V   +
Sbjct: 87  EADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGL 146

Query: 172 GDERCTLEDCRRMIADVDENGDGFVCFSEFVRMM 196
           G E+ T++ CRRMI  VD+NGDG + F EF  MM
Sbjct: 147 G-EKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

[Close]

BLAST of Moc01g17290.1 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 7.2e-19
Identity = 52/130 (40.00%), Postives = 79/130 (60.77%), Query Frame = 0


Query: 74  NNGVVSRKELEAILHRIGADPPSEEEVAMMLG-EFDGDGFIRLE---ALMDRVGSGFGPA 133
           N+G +S  EL+ ++  +  +   EE  AMM   + DG+GFI L+   AL          +
Sbjct: 27  NDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSNNS 86

Query: 134 CEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADVDENGDGFVCFS 193
              +L+EAF+ +D D +GRI+A ELH V  ++G E+C+++DC+RMI  VD +GDG V F 
Sbjct: 87  AIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVDFE 146

Query: 194 EFVRMMELQG 200
           EF +MM + G
Sbjct: 147 EFKKMMMING 155

[Close]

BLAST of Moc01g17290.1 vs. ExPASy TrEMBL
Match: A0A6J1DI26 (probable calcium-binding protein CML36 OS=Momordica charantia OX=3673 GN=LOC111021283 PE=4 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 8.0e-106
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTP 60
           MKIPKRFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTP
Sbjct: 1   MKIPKRFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTP 60

Query: 61  TSVLPDSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFIRLEALMD 120
           TSVLPDSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFIRLEALMD
Sbjct: 61  TSVLPDSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFIRLEALMD 120

Query: 121 RVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADVDE 180
           RVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADVDE
Sbjct: 121 RVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADVDE 180

Query: 181 NGDGFVCFSEFVRMMELQG 200
           NGDGFVCFSEFVRMMELQG
Sbjct: 181 NGDGFVCFSEFVRMMELQG 199

[Close]

BLAST of Moc01g17290.1 vs. ExPASy TrEMBL
Match: A0A6J1F310 (probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC111439413 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 2.2e-71
Identity = 155/200 (77.50%), Postives = 164/200 (82.00%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTP 60
           MKIPKRFSPKRLFRSKKN +    RSDPPSFGS TSSSSSSSENFFKP TA    GYGTP
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTA----GYGTP 60

Query: 61  TSVL--PDSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFIRLEAL 120
           TSVL    S +D  EN+GVVSRKELEAIL RI     SEEEVAMML E DG GFIRL+ L
Sbjct: 61  TSVLLAGCSDSDQRENDGVVSRKELEAILSRIA----SEEEVAMMLSEVDGGGFIRLDEL 120

Query: 121 MDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADV 180
           M RVGSGF    E+ELRE FEFFDADHDGRI+AEELHGV+ SIGDERCTLEDCRRMI DV
Sbjct: 121 MARVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDV 180

Query: 181 DENGDGFVCFSEFVRMMELQ 199
           DE+GDGFVCF+EFVRMMELQ
Sbjct: 181 DEDGDGFVCFTEFVRMMELQ 192

[Close]

BLAST of Moc01g17290.1 vs. ExPASy TrEMBL
Match: A0A6J1HS37 (probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC111467281 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 3.2e-70
Identity = 157/200 (78.50%), Postives = 165/200 (82.50%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKN-RADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGT 60
           MKIPKRFSPKRLFRSKKN  ADVS RSDPPSFGS TSSSSSSSENFFKP TA    GYGT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVS-RSDPPSFGSGTSSSSSSSENFFKPHTA----GYGT 60

Query: 61  PTSVLPD-SAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFIRLEAL 120
           PTSVL   S +D  EN+ VVSRKELEAIL RI     SEEEVAMML E D  GFIRL+ L
Sbjct: 61  PTSVLAGCSDSDQRENDVVVSRKELEAILSRIA----SEEEVAMMLSEVDDGGFIRLDEL 120

Query: 121 MDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADV 180
           M RVGSGF    E+ELRE FEFFDADHDGRI+AEELHGV+ SIGDERCTLEDCRRMI DV
Sbjct: 121 MARVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDV 180

Query: 181 DENGDGFVCFSEFVRMMELQ 199
           DE+GDGFVCF+EFVRMMELQ
Sbjct: 181 DEDGDGFVCFTEFVRMMELQ 191

[Close]

BLAST of Moc01g17290.1 vs. ExPASy TrEMBL
Match: A0A5A7VA05 (Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold382G00410 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 8.7e-68
Identity = 157/201 (78.11%), Postives = 166/201 (82.59%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNR-ADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGT 60
           MKIPKRFSPKRLFRSKK++ ADVS RSDPPSFGS TSSSSSSSENFFKP +  +  GYGT
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVS-RSDPPSFGSGTSSSSSSSENFFKPNSVNT--GYGT 61

Query: 61  PTSVLPDSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFD--GDGFIRLEA 120
           PTSVL    +D  EN GVV+RKELEAIL RI     SEEEVAMML E D  GDGFIRLE 
Sbjct: 62  PTSVLD---SDVRENEGVVTRKELEAILSRIA----SEEEVAMMLSEVDSGGDGFIRLEE 121

Query: 121 LMDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIAD 180
           LM RVGSG   A E+ELRE FEFFDAD DGRITAEELHGVF SIGDE+CTLEDCRRMI D
Sbjct: 122 LMARVGSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRD 181

Query: 181 VDENGDGFVCFSEFVRMMELQ 199
           VDENGDGFVCF+EFVRMMELQ
Sbjct: 182 VDENGDGFVCFAEFVRMMELQ 191

[Close]

BLAST of Moc01g17290.1 vs. ExPASy TrEMBL
Match: A0A1S3C5U3 (probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 8.7e-68
Identity = 157/201 (78.11%), Postives = 166/201 (82.59%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNR-ADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGT 60
           MKIPKRFSPKRLFRSKK++ ADVS RSDPPSFGS TSSSSSSSENFFKP +  +  GYGT
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVS-RSDPPSFGSGTSSSSSSSENFFKPNSVNT--GYGT 61

Query: 61  PTSVLPDSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFD--GDGFIRLEA 120
           PTSVL    +D  EN GVV+RKELEAIL RI     SEEEVAMML E D  GDGFIRLE 
Sbjct: 62  PTSVLD---SDVRENEGVVTRKELEAILSRIA----SEEEVAMMLSEVDSGGDGFIRLEE 121

Query: 121 LMDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIAD 180
           LM RVGSG   A E+ELRE FEFFDAD DGRITAEELHGVF SIGDE+CTLEDCRRMI D
Sbjct: 122 LMARVGSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRD 181

Query: 181 VDENGDGFVCFSEFVRMMELQ 199
           VDENGDGFVCF+EFVRMMELQ
Sbjct: 182 VDENGDGFVCFAEFVRMMELQ 191

[Close]

BLAST of Moc01g17290.1 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 179.5 bits (454), Expect = 2.7e-45
Identity = 110/213 (51.64%), Postives = 138/213 (64.79%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTP 60
           MK+ K   PKR F   K+R+ VS+     SFGSA+SSS    +N          GG  TP
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKN----SGGDGGGGSVTP 60

Query: 61  TSVLPDSAADWS-------------ENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEF 120
           TS+LP+  + +S             +N+G VSR +LE++L R+G DP +EEE+ +ML E 
Sbjct: 61  TSILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEV 120

Query: 121 --DGDGFIRLEALMDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDER 180
             DGDG IRLE L  RV S        EL+E FEFFDAD DG I+A+EL  VFS+IGDER
Sbjct: 121 DCDGDGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDER 180

Query: 181 CTLEDCRRMIADVDENGDGFVCFSEFVRMMELQ 199
           CTL+DC+RMIADVDE+GDGFVCF+EF RMM+LQ
Sbjct: 181 CTLDDCKRMIADVDEDGDGFVCFTEFSRMMDLQ 208

[Close]

BLAST of Moc01g17290.1 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 166.4 bits (420), Expect = 2.3e-41
Identity = 107/206 (51.94%), Postives = 130/206 (63.11%), Query Frame = 0


Query: 6   RFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTPTSVLP 65
           R SPKRLFR+ K++A VS RS+P SF S  SSSSS        Q     G   TP SVLP
Sbjct: 9   RLSPKRLFRT-KSKASVS-RSEPSSFSSNASSSSSDGSYGNLKQ-----GPTATPISVLP 68

Query: 66  DSAADW------------SENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDG--DG 125
            ++ D+             +++GVVSR +L A++ R+  +PPS+EEV++ML E DG   G
Sbjct: 69  QNSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGG 128

Query: 126 FIRLEALMDRVG--SGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLE 185
            I LE L  RV   SG G     ELRE FE FD D +G+I+AEELH VF  IGDERCTLE
Sbjct: 129 CISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLE 188

Query: 186 DCRRMIADVDENGDGFVCFSEFVRMM 196
           +C RMIA VD NGDGFVCF +F RMM
Sbjct: 189 ECMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Moc01g17290.1 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 95.5 bits (236), Expect = 5.1e-20
Identity = 52/130 (40.00%), Postives = 79/130 (60.77%), Query Frame = 0


Query: 74  NNGVVSRKELEAILHRIGADPPSEEEVAMMLG-EFDGDGFIRLE---ALMDRVGSGFGPA 133
           N+G +S  EL+ ++  +  +   EE  AMM   + DG+GFI L+   AL          +
Sbjct: 27  NDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSNNS 86

Query: 134 CEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADVDENGDGFVCFS 193
              +L+EAF+ +D D +GRI+A ELH V  ++G E+C+++DC+RMI  VD +GDG V F 
Sbjct: 87  AIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVDFE 146

Query: 194 EFVRMMELQG 200
           EF +MM + G
Sbjct: 147 EFKKMMMING 155

[Close]

BLAST of Moc01g17290.1 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 92.4 bits (228), Expect = 4.3e-19
Identity = 51/131 (38.93%), Postives = 79/131 (60.31%), Query Frame = 0


Query: 75  NGVVSRKELEAILHRIGADPPSEEEVAMMLG-EFDGDGFIRLEALMDRVGSGFGPACE-- 134
           +G +S  EL+ ++  +      EE V MM   + DG+GFI L+  +     G G      
Sbjct: 30  DGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGGGNNR 89

Query: 135 ---AELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADVDENGDGFVCF 194
              ++L+EAFE +D D +GRI+A+ELH V  ++G E+C+++DC++MI+ VD +GDG V F
Sbjct: 90  NDVSDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDIDGDGCVNF 149

Query: 195 SEFVRMMELQG 200
            EF +MM   G
Sbjct: 150 DEFKKMMSNGG 159

[Close]

BLAST of Moc01g17290.1 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 90.9 bits (224), Expect = 1.3e-18
Identity = 53/126 (42.06%), Postives = 77/126 (61.11%), Query Frame = 0


Query: 75  NGVVSRKELEAILHRIGADPPSEEEVAMMLGEFD--GDGFIRLEALMDRVGSGFGPACEA 134
           +G +S KEL AI+  +G + P EEE+   + E D  GDG+I  E  ++    G       
Sbjct: 50  DGKISSKELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVL 109

Query: 135 E-LREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRMIADVDENGDGFVCFSEF 194
           E L++AF  +D D +G I+AEELH V  S+GDE C++ +CR+MI  VD++GDG + F EF
Sbjct: 110 ENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEF 169

Query: 195 VRMMEL 198
             MM +
Sbjct: 170 KIMMTM 173

The following BLAST results are available for this feature:

BLAST of Moc01g17290.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153870.1	1.7e-105	100.00	probable calcium-binding protein CML36 [Momordica charantia]	[more]
XP_023543601.1	1.2e-71	79.10	probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo]	[more]
XP_022932888.1	4.6e-71	77.50	probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putati...	[more]
XP_022967907.1	6.6e-70	78.50	probable calcium-binding protein CML36 [Cucurbita maxima]	[more]
XP_008457589.1	1.8e-67	78.11	PREDICTED: probable calcium-binding protein CML36 [Cucumis melo] >KAA0062715.1 p...	[more]

BLAST of Moc01g17290.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SS31	3.8e-44	51.64	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
P30188	3.3e-40	51.94	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
Q5ZCK5	3.2e-19	48.85	Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8RZB5	5.5e-19	40.91	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9C8Y1	7.2e-19	40.00	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]

BLAST of Moc01g17290.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DI26	8.0e-106	100.00	probable calcium-binding protein CML36 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1F310	2.2e-71	77.50	probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1HS37	3.2e-70	78.50	probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC1114672...	[more]
A0A5A7VA05	8.7e-68	78.11	Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C5U3	8.7e-68	78.11	probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 P...	[more]

BLAST of Moc01g17290.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10190.1	2.7e-45	51.64	Calcium-binding EF-hand family protein	[more]
AT2G41410.1	2.3e-41	51.94	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	5.1e-20	40.00	calmodulin like 23	[more]
AT5G37770.1	4.3e-19	38.93	EF hand calcium-binding protein family	[more]
AT1G24620.1	1.3e-18	42.06	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 133..161 e-value: 3.8E-6 score: 36.4 coord: 170..198 e-value: 3.0E-5 score: 33.4
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 132..195 e-value: 2.6E-10 score: 40.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 129..164 score: 14.987627
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 166..199 score: 13.732334
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 133..195 e-value: 1.29732E-19 score: 76.8177
None	No IPR available	GENE3D	1.10.238.10		coord: 126..199 e-value: 4.3E-21 score: 77.5
None	No IPR available	GENE3D	1.10.238.10		coord: 70..121 e-value: 9.3E-8 score: 34.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 28..57
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..59
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 2..197
None	No IPR available	PANTHER	PTHR10891:SF790	CALCIUM-BINDING PROTEIN CML35-RELATED	coord: 2..197
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 142..154
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 179..191
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 73..195

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc01g17290	Moc01g17290	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc01g17290.1.exon1	Moc01g17290.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc01g17290.1	cds.Moc01g17290.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc01g17290.1	Moc01g17290.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding