MS028609.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS028609.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNase H domain-containing protein
Location	scaffold78: 634744 .. 635388 (+)
Sequence length	645
RNA-Seq Expression	MS028609.1
Synteny	MS028609.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGCGTAATCAAACTCCCCAACATTTCTCGTTGGGTGGCCTTTTGTCGGACATTGTTTCTTGGTCGGAAAATTACCTCGAGTTTATCAAGCGGCTCAACGGAGTTCTGCGTCTGCTCCAGTGGTTTCTCGTCCACCTCGGGTGTCTTTCTGGTGTCCTTTTGCAGCGCCTCTCCTCAAGGTGAATGTGGATGCAGCTTTCAGAAAGAATCTTTTGTAGTAGGGGTGAGTGTAATTATTCGGGATTCGGTCGGTCTTGTGTATCTTACGGTCGTTCGTCCGCTTGCTCGTGCTCGTGATGTTGATTGGGTGGAAGGCTTTGCGGTTTATGAGGGTATTCTCCTTGCTGTGGAAGCAGGTTTTATTCGGTTCCAGATCGAAACAGATTCACTACGGATTTTTAATTTACTGACGATAGATTGTGTGGATGATTTTGAAGTTGGGACTCTCTACTCTGTCATCAAGCTTTTTTTGTCTTCTCATGGTAGGTGGGTTTCTTTTAGTTTTACACGTAGGGACGGTAATGCTGCAGCCCATCTGTTAGCTCAACTGGCATTGACTTCACCACACCTTCAAATCTGGGTGGAGGAATGGCCTGATGAGATCTCTTTGATGTTGGCGGTGGATTGTAGTTCCTGTTCTTAA

mRNA sequence

ATGCGCGTAATCAAACTCCCCAACATTTCTCGTTGGGTGGCCTTTTGTCGGACATTGTTTCTTGGTCGGAAAATTACCTCGAGTTTATCAAGCGGCTCAACGGAGTTCTGCGTCTGCTCCAGTGGTTTCTCGTCCACCTCGGGTGTCTTTCTGGTGTCCTTTTGCAGCGCCTCTCCTCAAGGTGAATGTGGATGCAGCTTTCAGAAAGAATCTTTTGTAGTAGGGGTGAGTGTAATTATTCGGGATTCGGTCGGTCTTGTGTATCTTACGGTCGTTCGTCCGCTTGCTCGTGCTCGTGATGTTGATTGGGTGGAAGGCTTTGCGGTTTATGAGGGTATTCTCCTTGCTGTGGAAGCAGGTTTTATTCGGTTCCAGATCGAAACAGATTCACTACGGATTTTTAATTTACTGACGATAGATTGTGTGGATGATTTTGAAGTTGGGACTCTCTACTCTGTCATCAAGCTTTTTTTGTCTTCTCATGGTAGGTGGGTTTCTTTTAGTTTTACACGTAGGGACGGTAATGCTGCAGCCCATCTGTTAGCTCAACTGGCATTGACTTCACCACACCTTCAAATCTGGGTGGAGGAATGGCCTGATGAGATCTCTTTGATGTTGGCGGTGGATTGTAGTTCCTGTTCTTAA

Coding sequence (CDS)

ATGCGCGTAATCAAACTCCCCAACATTTCTCGTTGGGTGGCCTTTTGTCGGACATTGTTTCTTGGTCGGAAAATTACCTCGAGTTTATCAAGCGGCTCAACGGAGTTCTGCGTCTGCTCCAGTGGTTTCTCGTCCACCTCGGGTGTCTTTCTGGTGTCCTTTTGCAGCGCCTCTCCTCAAGGTGAATGTGGATGCAGCTTTCAGAAAGAATCTTTTGTAGTAGGGGTGAGTGTAATTATTCGGGATTCGGTCGGTCTTGTGTATCTTACGGTCGTTCGTCCGCTTGCTCGTGCTCGTGATGTTGATTGGGTGGAAGGCTTTGCGGTTTATGAGGGTATTCTCCTTGCTGTGGAAGCAGGTTTTATTCGGTTCCAGATCGAAACAGATTCACTACGGATTTTTAATTTACTGACGATAGATTGTGTGGATGATTTTGAAGTTGGGACTCTCTACTCTGTCATCAAGCTTTTTTTGTCTTCTCATGGTAGGTGGGTTTCTTTTAGTTTTACACGTAGGGACGGTAATGCTGCAGCCCATCTGTTAGCTCAACTGGCATTGACTTCACCACACCTTCAAATCTGGGTGGAGGAATGGCCTGATGAGATCTCTTTGATGTTGGCGGTGGATTGTAGTTCCTGTTCTTAA

Protein sequence

MRVIKLPNISRWVAFCRTLFLGRKITSSLSSGSTEFCVCSSGFSSTSGVFLVSFCSASPQGECGCSFQKESFVVGVSVIIRDSVGLVYLTVVRPLARARDVDWVEGFAVYEGILLAVEAGFIRFQIETDSLRIFNLLTIDCVDDFEVGTLYSVIKLFLSSHGRWVSFSFTRRDGNAAAHLLAQLALTSPHLQIWVEEWPDEISLMLAVDCSSCS

Homology

[Close]

BLAST of MS028609.1 vs. NCBI nr
Match: XP_022150944.1 (uncharacterized protein LOC111018973 [Momordica charantia])

HSP 1 Score: 246.9 bits (629), Expect = 1.6e-61
Identity = 126/149 (84.56%), Postives = 131/149 (87.92%), Query Frame = 0


Query: 66  SFQKESFVVGVSVIIRDSVGLVYLTVVRPLARARDVDWVEGFAVYEGILLAVEAGFIRFQ 125
           +F+KESFV GV VIIRDS GLVYLT +R LARA DVDWVEGFAVYEGILLAVEAGFIRFQ
Sbjct: 119 AFRKESFVAGVGVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVYEGILLAVEAGFIRFQ 178

Query: 126 IETDSLRIFNLLTIDCVDDFEVGTLYSVIKLFLSSHGRWVSFSFTRRDGNAAAHLLAQLA 185
           IETDSLRIFNLLT DCVDD EVG L SVIKLFLSSH   VSFSFT R+GNA AHLLAQLA
Sbjct: 179 IETDSLRIFNLLTTDCVDDSEVGVLCSVIKLFLSSHAERVSFSFTHRNGNAXAHLLAQLA 238

Query: 186 LTSPHLQIWVEEWPDEISLMLAVDCSSCS 215
           LTSPHLQIWVEEWPDEIS +LAVDC S S
Sbjct: 239 LTSPHLQIWVEEWPDEISSVLAVDCRSRS 267

[Close]

BLAST of MS028609.1 vs. NCBI nr
Match: XP_022135942.1 (uncharacterized protein LOC111007775 [Momordica charantia])

HSP 1 Score: 122.9 bits (307), Expect = 3.4e-24
Identity = 62/144 (43.06%), Postives = 89/144 (61.81%), Query Frame = 0


Query: 66  SFQKESFVVGVSVIIRDSVGLVYLTVVRPLARARDVDWVEGFAVYEGILLAVEAGFIRFQ 125
           +F+K +F  G+ ++IRDS   V L+ +  +    DV   E  A  EG+ LA+EAG I FQ
Sbjct: 88  AFKKGNFSAGLDILIRDSTVNVLLSAMCFITHVSDVTLAECLAAQEGVCLAIEAGLIPFQ 147

Query: 126 IETDSLRIFNLLTIDCVDDFEVGTLYSVIKLFLSSHGRWVSFSFTRRDGNAAAHLLAQLA 185
           IETDS ++FNLL  DC D+ E+G L S I+  +SS      FSF  R+GN+ AH LA++ 
Sbjct: 148 IETDSSQVFNLLRTDCEDESEIGVLASSIRHIVSSLHIGGGFSFVNREGNSGAHTLARMG 207

Query: 186 LTSPHLQIWVEEWPDEISLMLAVD 210
           + S    +WVEEW  ++S ++A D
Sbjct: 208 MVSESFHVWVEEWLSDLSEVIAAD 231

[Close]

BLAST of MS028609.1 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 88.2 bits (217), Expect = 9.4e-14
Identity = 52/145 (35.86%), Postives = 74/145 (51.03%), Query Frame = 0


Query: 66  SFQKESFVVGVSVIIRDSVGLVYLTVVRPLARARDVDWVEGFAVYEGILLAVEAGFIRFQ 125
           SF       G+ +IIR+  G V  +  + L   + VD  E     EG+ LA + G     
Sbjct: 49  SFLASDQHAGLGIIIRNDRGQVMASATKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVI 108

Query: 126 IETDSLRIFNLLTIDCVDDFEVGTLYSVIKLFLSSHGRWVSFSFTRRDGNAAAHLLAQLA 185
           +ETDS RIFNL +    D  E G +    K F  +     SF+F +R+GN AAH+LA+ A
Sbjct: 109 LETDSSRIFNLFSQPSEDLSETGEIVLKAKNFW-TQSLHASFNFVKREGNKAAHMLARRA 168

Query: 186 LTSPHLQIWVEEWPDEISLMLAVDC 211
           L      IW+E+WP E+   L ++C
Sbjct: 169 LLLREFSIWMEDWPLELKSCLEMEC 192

[Close]

BLAST of MS028609.1 vs. NCBI nr
Match: XP_016173756.1 (uncharacterized protein LOC107616295 [Arachis ipaensis] >XP_025679120.1 uncharacterized protein LOC112779076 [Arachis hypogaea])

HSP 1 Score: 83.6 bits (205), Expect = 2.3e-12
Identity = 55/140 (39.29%), Postives = 76/140 (54.29%), Query Frame = 0


Query: 75  GVSVIIRDSVGLVYLTVVRPLARARDVDWVEGFAVYEGILLAVEAGFIRFQIETDSLRIF 134
           GV V+IRDS GLV       +A A  V   E FA Y+G+ +A +  F+  +IE D++ + 
Sbjct: 133 GVGVVIRDSNGLVVAAATLEVAPALSVREAEAFAFYQGVNIAAQTCFLEIEIEGDNIEVV 192

Query: 135 NLLTIDCVDDFEVGTLYSVIKLFLSSHGRWVSFSFTRRDGNAAAHLLAQLALTSPHLQIW 194
           N L  +       GT+ S  K  L    R+   S  +R+ N+ AH LA+LALT P+L +W
Sbjct: 193 NALNSNNPFGGTFGTIISNCKALLCCF-RFYKISHIKRNKNSVAHSLAKLALTRPNL-MW 252

Query: 195 VEEWPDEISLMLAVDCSSCS 215
           +EE P E  L L V C S S
Sbjct: 253 LEETPTE-HLALVVACPSPS 269

[Close]

BLAST of MS028609.1 vs. NCBI nr
Match: RYQ93089.1 (hypothetical protein Ahy_B09g099349 [Arachis hypogaea])

HSP 1 Score: 83.6 bits (205), Expect = 2.3e-12
Identity = 55/140 (39.29%), Postives = 76/140 (54.29%), Query Frame = 0


Query: 75  GVSVIIRDSVGLVYLTVVRPLARARDVDWVEGFAVYEGILLAVEAGFIRFQIETDSLRIF 134
           GV V+IRDS GLV       +A A  V   E FA Y+G+ +A +  F+  +IE D++ + 
Sbjct: 174 GVGVVIRDSNGLVVAAATLEVAPALSVREAEAFAFYQGVNIAAQTCFLEIEIEGDNIEVV 233

Query: 135 NLLTIDCVDDFEVGTLYSVIKLFLSSHGRWVSFSFTRRDGNAAAHLLAQLALTSPHLQIW 194
           N L  +       GT+ S  K  L    R+   S  +R+ N+ AH LA+LALT P+L +W
Sbjct: 234 NALNSNNPFGGTFGTIISNCKALLCCF-RFYKISHIKRNKNSVAHSLAKLALTRPNL-MW 293

Query: 195 VEEWPDEISLMLAVDCSSCS 215
           +EE P E  L L V C S S
Sbjct: 294 LEETPTE-HLALVVACPSPS 310

[Close]

BLAST of MS028609.1 vs. ExPASy TrEMBL
Match: A0A6J1DBJ7 (uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018973 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 7.6e-62
Identity = 126/149 (84.56%), Postives = 131/149 (87.92%), Query Frame = 0


Query: 66  SFQKESFVVGVSVIIRDSVGLVYLTVVRPLARARDVDWVEGFAVYEGILLAVEAGFIRFQ 125
           +F+KESFV GV VIIRDS GLVYLT +R LARA DVDWVEGFAVYEGILLAVEAGFIRFQ
Sbjct: 119 AFRKESFVAGVGVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVYEGILLAVEAGFIRFQ 178

Query: 126 IETDSLRIFNLLTIDCVDDFEVGTLYSVIKLFLSSHGRWVSFSFTRRDGNAAAHLLAQLA 185
           IETDSLRIFNLLT DCVDD EVG L SVIKLFLSSH   VSFSFT R+GNA AHLLAQLA
Sbjct: 179 IETDSLRIFNLLTTDCVDDSEVGVLCSVIKLFLSSHAERVSFSFTHRNGNAXAHLLAQLA 238

Query: 186 LTSPHLQIWVEEWPDEISLMLAVDCSSCS 215
           LTSPHLQIWVEEWPDEIS +LAVDC S S
Sbjct: 239 LTSPHLQIWVEEWPDEISSVLAVDCRSRS 267

[Close]

BLAST of MS028609.1 vs. ExPASy TrEMBL
Match: A0A6J1C467 (uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007775 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 1.7e-24
Identity = 62/144 (43.06%), Postives = 89/144 (61.81%), Query Frame = 0


Query: 66  SFQKESFVVGVSVIIRDSVGLVYLTVVRPLARARDVDWVEGFAVYEGILLAVEAGFIRFQ 125
           +F+K +F  G+ ++IRDS   V L+ +  +    DV   E  A  EG+ LA+EAG I FQ
Sbjct: 88  AFKKGNFSAGLDILIRDSTVNVLLSAMCFITHVSDVTLAECLAAQEGVCLAIEAGLIPFQ 147

Query: 126 IETDSLRIFNLLTIDCVDDFEVGTLYSVIKLFLSSHGRWVSFSFTRRDGNAAAHLLAQLA 185
           IETDS ++FNLL  DC D+ E+G L S I+  +SS      FSF  R+GN+ AH LA++ 
Sbjct: 148 IETDSSQVFNLLRTDCEDESEIGVLASSIRHIVSSLHIGGGFSFVNREGNSGAHTLARMG 207

Query: 186 LTSPHLQIWVEEWPDEISLMLAVD 210
           + S    +WVEEW  ++S ++A D
Sbjct: 208 MVSESFHVWVEEWLSDLSEVIAAD 231

[Close]

BLAST of MS028609.1 vs. ExPASy TrEMBL
Match: A0A6J1CIF1 (uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011237 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 4.5e-14
Identity = 52/145 (35.86%), Postives = 74/145 (51.03%), Query Frame = 0


Query: 66  SFQKESFVVGVSVIIRDSVGLVYLTVVRPLARARDVDWVEGFAVYEGILLAVEAGFIRFQ 125
           SF       G+ +IIR+  G V  +  + L   + VD  E     EG+ LA + G     
Sbjct: 49  SFLASDQHAGLGIIIRNDRGQVMASATKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVI 108

Query: 126 IETDSLRIFNLLTIDCVDDFEVGTLYSVIKLFLSSHGRWVSFSFTRRDGNAAAHLLAQLA 185
           +ETDS RIFNL +    D  E G +    K F  +     SF+F +R+GN AAH+LA+ A
Sbjct: 109 LETDSSRIFNLFSQPSEDLSETGEIVLKAKNFW-TQSLHASFNFVKREGNKAAHMLARRA 168

Query: 186 LTSPHLQIWVEEWPDEISLMLAVDC 211
           L      IW+E+WP E+   L ++C
Sbjct: 169 LLLREFSIWMEDWPLELKSCLEMEC 192

[Close]

BLAST of MS028609.1 vs. ExPASy TrEMBL
Match: A0A444XTI4 (RNase H domain-containing protein OS=Arachis hypogaea OX=3818 GN=Ahy_B09g099349 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.1e-12
Identity = 55/140 (39.29%), Postives = 76/140 (54.29%), Query Frame = 0


Query: 75  GVSVIIRDSVGLVYLTVVRPLARARDVDWVEGFAVYEGILLAVEAGFIRFQIETDSLRIF 134
           GV V+IRDS GLV       +A A  V   E FA Y+G+ +A +  F+  +IE D++ + 
Sbjct: 174 GVGVVIRDSNGLVVAAATLEVAPALSVREAEAFAFYQGVNIAAQTCFLEIEIEGDNIEVV 233

Query: 135 NLLTIDCVDDFEVGTLYSVIKLFLSSHGRWVSFSFTRRDGNAAAHLLAQLALTSPHLQIW 194
           N L  +       GT+ S  K  L    R+   S  +R+ N+ AH LA+LALT P+L +W
Sbjct: 234 NALNSNNPFGGTFGTIISNCKALLCCF-RFYKISHIKRNKNSVAHSLAKLALTRPNL-MW 293

Query: 195 VEEWPDEISLMLAVDCSSCS 215
           +EE P E  L L V C S S
Sbjct: 294 LEETPTE-HLALVVACPSPS 310

[Close]

BLAST of MS028609.1 vs. ExPASy TrEMBL
Match: A0A803LHG6 (Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 1.5e-12
Identity = 50/141 (35.46%), Postives = 77/141 (54.61%), Query Frame = 0


Query: 74  VGVSVIIRDSVGLVYLTVVRPLARARDVDWVEGFAVYEGILLAVEAGFIRFQIETDSLRI 133
           VG   ++RD+VG V       +    DVD VE  A+   + +A+E+GF+R  +ETD+L++
Sbjct: 226 VGFGGVMRDNVGDVVAATCLQIVGRFDVDVVEAMAMRHALKIAMESGFVRLCLETDNLKL 285

Query: 134 FNLLTIDCVDDFEVGTLYSVIKLFLSSHGRWVSFSFTRRDGNAAAHLLAQLALTSPHLQI 193
            + L     D    G +   I L L+S      F F +R+GN  AH LA+L+ T   L++
Sbjct: 286 HSHLKKSKYDQTTFGIVVKDI-LTLASSCHSCVFEFVKREGNKVAHSLAKLSSTFDTLRV 345

Query: 194 WVEEWPDEISLMLAVDCSSCS 215
           W+EE+P  I  ++  D SS S
Sbjct: 346 WLEEYPSGIHELVMADSSSSS 365

The following BLAST results are available for this feature:

BLAST of MS028609.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150944.1	1.6e-61	84.56	uncharacterized protein LOC111018973 [Momordica charantia]	[more]
XP_022135942.1	3.4e-24	43.06	uncharacterized protein LOC111007775 [Momordica charantia]	[more]
XP_022140628.1	9.4e-14	35.86	uncharacterized protein LOC111011237 [Momordica charantia]	[more]
XP_016173756.1	2.3e-12	39.29	uncharacterized protein LOC107616295 [Arachis ipaensis] >XP_025679120.1 uncharac...	[more]
RYQ93089.1	2.3e-12	39.29	hypothetical protein Ahy_B09g099349 [Arachis hypogaea]	[more]

BLAST of MS028609.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS028609.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DBJ7	7.6e-62	84.56	uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018...	[more]
A0A6J1C467	1.7e-24	43.06	uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007...	[more]
A0A6J1CIF1	4.5e-14	35.86	uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A444XTI4	1.1e-12	39.29	RNase H domain-containing protein OS=Arachis hypogaea OX=3818 GN=Ahy_B09g099349 ...	[more]
A0A803LHG6	1.5e-12	35.46	Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1	[more]

BLAST of MS028609.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 69..185 e-value: 2.0E-14 score: 53.4
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 61..187 e-value: 5.7E-5 score: 25.2
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 73..190

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS028609	MS028609	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS028609.1-cds	MS028609.1-cds-scaffold78:634744..635388	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS028609.1	MS028609.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity